Rroxscaffold_7G00200820

Cytochrome c oxidase assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001655
Physical Location & Seq
Forward (+)
48220003 .. 48223191
3189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00200820.1

Sequence Viewer

Length: 1257 bp
ATGTTTCGGAGCCGAGTGATCGCGTTGTTTGTGAAGAACAATAGGAAGGCTCTCTACAATGTGAGGGCATCTAGGGTTTCCAATGAGGAGGCTACCTACCGCCTATTCTCTACTGGGCTCACAAGGAAATGCTTCCCATCTTTCTCCAAGGGTAGCTATGTATCATCTATAAGGAATATGTCCACTGTGGCTTCTGTAGGCATCAAAGATAAGGAGGGATTGAAGTTTCTTGTAAACGGAGGACCTCGTGCTCAGAAATTGATTGGCATTTGGCTTTTCGGCTCCGCTGCATGGGTGTTTAGCATGGTGATACTTGGAGACTGGTTGATTGAGTTCCAAAAGTACAAGCAATCCCCTGAATATAAGCGTGTAAATAAAGGGATGAGTATTGAAGACTTCAAATTTATCTACTGGATGGAATATGCACATCGTATGTGGGGAAGGGCATTGGGTATTATGTTCGCTTTGCCATTCTCATATTTTCTTCGTAAGGGTTATATTACCTTACAACTTGGACTGAGACTTTCTACTCTTCTTGCCCTTGGTGCTGGGCAGGGTCTAATTGGTTGGTGGATGGTTAAAAGTGGTTTAGAGGAGCCAGCATCTGAGTATGCTCAGCCAAGAGTAAGCCCTTACCGATTAGCAGCTCACCTTACTTCAGCCTTTGCTATATACTGTGGCCTTTTGTGGACTGGTCTTTCTGTTGTCATGCCTGAACCACCTGCTGAATCTGTAGCCTGGGTTCATGGTGCTGCAAAAGTGAAGAGACTTGCTCTTCCTGTAAGCTTACTGGTTGGGATTACTGCTATCTCAGGAGCATTTGTTGCGGGAATGATGCTGTATGGACATGCTTATAATACTTTTCCCAAGATGGGCGACAGATGGATCCCAGAGGATGTTTTTGACATGAAGCCACTAATCCGGAACTTCTTTGAGAATACATCTACTGTGCAGCTTGACCACCGTATGCTTGCAACTGCAACTTTATTTTCAATTGTCGCTTTGTGGTGGTCAACAAGGAAGTTTGACATACACCCTGCAGTTCGATCGTTGATTGGAACTACTGTTGGCATGGCTGGCCTTCAGGTCACACTGGGGATATCTACACTTCTGTCGTATGTGCCTGTTGAGCTTGGCACTGCACATCAAGCTGGGGCATTGACTCTCTTCACACTTATGATCCTTCTCAATCACATTGTGAGGAAGCCATCACCATCCCTTCTTAAATCTCTACCTCAAGTAGCAAAGACAATATAA

Protein Analysis

418

Amino Acids

46.63

Weight (kDa)

10.04

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COX15-CtaA PF02628 107 - 394 1.3e-87 Cytochrome oxidase assembly protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 855
AarI CACCTGC 1 cut(s) 730
Acc36I ACCTGC 1 cut(s) 730
AccII CGCG 1 cut(s) 23
AccIII TCCGGA 1 cut(s) 921
AciI CCGC 3 cut(s) 100, 285, 827
AclWI GGATC 3 cut(s) 880, 893, 1174
AcsI RAATTY 1 cut(s) 401
AcuI CTGAAG 2 cut(s) 642, 1067
AdeI CACNNNGTG 1 cut(s) 1198
AfaI GTAC 1 cut(s) 344
AfiI CCNNNNNNNGG 2 cut(s) 291, 872
AgsI TTSAA 4 cut(s) 223, 392, 400, 993
AjnI CCWGG 1 cut(s) 737
AluBI AGCT 6 cut(s) 156, 647, 786, 955, 1132, 1151
AluI AGCT 6 cut(s) 156, 647, 786, 955, 1132, 1151
Alw21I GWGCWC 1 cut(s) 253
Alw26I GTCTC 3 cut(s) 312, 514, 760
AlwI GGATC 3 cut(s) 880, 893, 1174
AlwNI CAGNNNCTG 1 cut(s) 605
Aor13HI TCCGGA 1 cut(s) 921
AoxI GGCC 2 cut(s) 679, 1078
ApeKI GCWGC 4 cut(s) 287, 644, 752, 952
ApoI RAATTY 1 cut(s) 401
Asp700I GAANNNNTTC 1 cut(s) 131
AspS9I GGNCC 1 cut(s) 242
AsuHPI GGTGA 3 cut(s) 319, 641, 1203
AvaII GGWCC 1 cut(s) 242
BamHI GGATCC 1 cut(s) 885
BanII GRGCYC 1 cut(s) 120
BarI GAAGNNNNNNTAC 2 cut(s) 38, 70
BauI CACGAG 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 399
Bbv12I GWGCWC 1 cut(s) 253
BbvI GCAGC 4 cut(s) 274, 656, 739, 964
BccI CCATC 7 cut(s) 145, 409, 568, 865, 876, 1216, 1222
BcgI CGANNNNNNTGC 2 cut(s) 1029, 1063
BciT130I CCWGG 1 cut(s) 739
BcoDI GTCTC 3 cut(s) 312, 514, 760
BfaI CTAG 1 cut(s) 72
BfmI CTRYAG 3 cut(s) 195, 732, 1038
BfuAI ACCTGC 1 cut(s) 730
BisI GCNGC 4 cut(s) 288, 645, 753, 953
BlpI GCTNAGC 1 cut(s) 615
BlsI GCNGC 4 cut(s) 289, 646, 754, 954
Bme1390I CCNGG 1 cut(s) 739
Bme18I GGWCC 1 cut(s) 242
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 4 cut(s) 11, 283, 597, 887
BmrFI CCNGG 1 cut(s) 739
BmrI ACTGGG 2 cut(s) 123, 1103
BmsI GCATC 4 cut(s) 77, 210, 611, 825
BmuI ACTGGG 2 cut(s) 123, 1103
BpiI GAAGAC 1 cut(s) 399
BplI GAGNNNNNCTC 2 cut(s) 757, 789
Bpu1102I GCTNAGC 1 cut(s) 615
BpuEI CTTGAG 1 cut(s) 1221
BsaJI CCNNGG 3 cut(s) 147, 541, 738
BsaWI WCCGGW 1 cut(s) 921
BsaXI ACNNNNNCTCC 2 cut(s) 807, 837
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 872
Bse1I ACTGG 6 cut(s) 118, 326, 416, 697, 795, 1098
BseAI TCCGGA 1 cut(s) 921
BseBI CCWGG 1 cut(s) 739
BseDI CCNNGG 3 cut(s) 147, 541, 738
BseGI GGATG 5 cut(s) 387, 420, 579, 901, 1214
BseLI CCNNNNNNNGG 2 cut(s) 291, 872
BseMII CTCAG 5 cut(s) 266, 509, 597, 629, 825
BseNI ACTGG 6 cut(s) 118, 326, 416, 697, 795, 1098
BseRI GAGGAG 2 cut(s) 101, 608
BseXI GCAGC 4 cut(s) 274, 656, 739, 964
BseYI CCCAGC 2 cut(s) 548, 1151
BsgI GTGCAG 2 cut(s) 971, 1125
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 1 cut(s) 1049
BshFI GGCC 2 cut(s) 681, 1080
BsiEI CGRYCG 1 cut(s) 1049
BsiHKAI GWGCWC 1 cut(s) 253
BsiSI CCGG 1 cut(s) 922
BslI CCNNNNNNNGG 2 cut(s) 291, 872
BsmAI GTCTC 3 cut(s) 312, 514, 760
BsnI GGCC 2 cut(s) 681, 1080
Bsp1286I GDGCHC 2 cut(s) 120, 253
Bsp13I TCCGGA 1 cut(s) 921
Bsp143I GATC 4 cut(s) 18, 885, 1046, 1179
Bsp1720I GCTNAGC 1 cut(s) 615
BspACI CCGC 3 cut(s) 100, 285, 827
BspANI GGCC 2 cut(s) 681, 1080
BspCNI CTCAG 5 cut(s) 265, 510, 598, 628, 824
BspEI TCCGGA 1 cut(s) 921
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 4 cut(s) 11, 283, 597, 887
BspMAI CTGCAG 1 cut(s) 1042
BspMI ACCTGC 1 cut(s) 730
BspPI GGATC 3 cut(s) 880, 893, 1174
BspQI GCTCTTC 1 cut(s) 780
BsrI ACTGG 6 cut(s) 118, 326, 416, 697, 795, 1098
BssECI CCNNGG 3 cut(s) 147, 541, 738
BssMI GATC 4 cut(s) 18, 885, 1046, 1179
BssSI CACGAG 1 cut(s) 246
BssT1I CCWWGG 2 cut(s) 147, 541
Bst2BI CACGAG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 739
Bst4CI ACNGT 5 cut(s) 187, 677, 949, 965, 1066
Bst6I CTCTTC 4 cut(s) 537, 758, 780, 1172
BstAPI GCANNNNNTGC 1 cut(s) 824
BstC8I GCNNGC 3 cut(s) 600, 972, 1078
BstDEI CTNAG 5 cut(s) 252, 518, 606, 615, 811
BstF5I GGATG 5 cut(s) 387, 420, 579, 901, 1214
BstFNI CGCG 1 cut(s) 23
BstKTI GATC 4 cut(s) 21, 888, 1049, 1182
BstMAI GTCTC 3 cut(s) 312, 514, 760
BstMBI GATC 4 cut(s) 18, 885, 1046, 1179
BstMCI CGRYCG 1 cut(s) 1049
BstMWI GCNNNNNNNGC 5 cut(s) 545, 824, 1077, 1129, 1148
BstNI CCWGG 1 cut(s) 739
BstNSI RCATGY 1 cut(s) 851
BstSCI CCNGG 1 cut(s) 737
BstSFI CTRYAG 3 cut(s) 195, 732, 1038
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 4 cut(s) 274, 656, 739, 964
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 1 cut(s) 885
BstYI RGATCY 1 cut(s) 885
BsuRI GGCC 2 cut(s) 681, 1080
BtsCI GGATG 5 cut(s) 387, 420, 579, 901, 1214
BtsI GCAGTG 1 cut(s) 1137
BtsIMutI CAGTG 3 cut(s) 183, 1091, 1137
BveI ACCTGC 1 cut(s) 730
Cac8I GCNNGC 3 cut(s) 600, 972, 1078
CaiI CAGNNNCTG 1 cut(s) 605
Cfr13I GGNCC 1 cut(s) 242
Csp6I GTAC 1 cut(s) 343
CviAII CATG 7 cut(s) 291, 304, 709, 746, 848, 907, 1072
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 5 cut(s) 252, 518, 606, 615, 811
DpnI GATC 4 cut(s) 20, 887, 1048, 1181
DpnII GATC 4 cut(s) 18, 885, 1046, 1179
DraIII CACNNNGTG 1 cut(s) 1198
Eam1104I CTCTTC 4 cut(s) 537, 758, 780, 1172
EarI CTCTTC 4 cut(s) 537, 758, 780, 1172
Eco130I CCWWGG 2 cut(s) 147, 541
Eco24I GRGCYC 1 cut(s) 120
Eco32I GATATC 1 cut(s) 1101
Eco47I GGWCC 1 cut(s) 242
Eco57I CTGAAG 2 cut(s) 642, 1067
EcoO109I RGGNCCY 1 cut(s) 242
EcoRII CCWGG 1 cut(s) 737
EcoRV GATATC 1 cut(s) 1101
EcoT14I CCWWGG 2 cut(s) 147, 541
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 2 cut(s) 147, 541
FaeI CATG 7 cut(s) 294, 307, 712, 749, 851, 910, 1075
FatI CATG 7 cut(s) 290, 303, 708, 745, 847, 906, 1071
FauI CCCGC 1 cut(s) 820
Fnu4HI GCNGC 4 cut(s) 288, 645, 753, 953
FokI GGATG 5 cut(s) 394, 427, 586, 908, 1201
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 4 cut(s) 288, 645, 753, 953
FspBI CTAG 1 cut(s) 72
GluI GCNGC 4 cut(s) 288, 645, 753, 953
GsaI CCCAGC 2 cut(s) 552, 1155
HaeIII GGCC 2 cut(s) 681, 1080
HapII CCGG 1 cut(s) 922
Hin1II CATG 7 cut(s) 294, 307, 712, 749, 851, 910, 1075
HincII GTYRAC 1 cut(s) 1014
HindII GTYRAC 1 cut(s) 1014
HindIII AAGCTT 1 cut(s) 784
HinfI GANTC 2 cut(s) 728, 1162
HpaII CCGG 1 cut(s) 922
HphI GGTGA 3 cut(s) 319, 641, 1203
Hpy166II GTNNAC 4 cut(s) 183, 235, 690, 1014
Hpy188I TCNGA 3 cut(s) 9, 255, 607
Hpy188III TCNNGA 2 cut(s) 813, 922
Hpy8I GTNNAC 4 cut(s) 183, 235, 690, 1014
HpyAV CCTTC 5 cut(s) 40, 435, 1091, 1193, 1229
HpyCH4III ACNGT 5 cut(s) 187, 677, 949, 965, 1066
HpyCH4V TGCA 8 cut(s) 290, 425, 755, 952, 974, 980, 1040, 1142
HpyF10VI GCNNNNNNNGC 5 cut(s) 545, 824, 1077, 1129, 1148
HpyF3I CTNAG 5 cut(s) 252, 518, 606, 615, 811
Hsp92II CATG 7 cut(s) 294, 307, 712, 749, 851, 910, 1075
Kpn2I TCCGGA 1 cut(s) 921
Kzo9I GATC 4 cut(s) 18, 885, 1046, 1179
LguI GCTCTTC 1 cut(s) 780
LmnI GCTCC 4 cut(s) 9, 287, 595, 815
Lsp1109I GCAGC 4 cut(s) 274, 656, 739, 964
LweI GCATC 4 cut(s) 77, 210, 611, 825
MaeI CTAG 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 1087
MalI GATC 4 cut(s) 20, 887, 1048, 1181
MboI GATC 4 cut(s) 18, 885, 1046, 1179
MboII GAAGA 7 cut(s) 46, 404, 476, 524, 767, 775, 1159
MfeI CAATTG 1 cut(s) 993
MflI RGATCY 1 cut(s) 885
MhlI GDGCHC 2 cut(s) 120, 253
MluCI AATT 4 cut(s) 257, 401, 561, 993
MlyI GAGTC 1 cut(s) 1156
MroI TCCGGA 1 cut(s) 921
MroXI GAANNNNTTC 1 cut(s) 131
MseI TTAA 2 cut(s) 579, 1224
MslI CAYNNNNRTG 1 cut(s) 1175
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 1 cut(s) 922
MspR9I CCNGG 1 cut(s) 739
MunI CAATTG 1 cut(s) 993
MvaI CCWGG 1 cut(s) 739
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 5 cut(s) 545, 824, 1077, 1129, 1148
NdeII GATC 4 cut(s) 18, 885, 1046, 1179
NlaIII CATG 7 cut(s) 294, 307, 712, 749, 851, 910, 1075
NlaIV GGNNCC 4 cut(s) 11, 283, 597, 887
NmeAIII GCCGAG 1 cut(s) 38
NmuCI GTSAC 1 cut(s) 1087
NspI RCATGY 1 cut(s) 851
PaqCI CACCTGC 1 cut(s) 730
PciSI GCTCTTC 1 cut(s) 780
PdmI GAANNNNTTC 1 cut(s) 131
PfeI GAWTC 1 cut(s) 728
PkrI GCNGC 4 cut(s) 289, 646, 754, 954
Ple19I CGATCG 1 cut(s) 1049
PleI GAGTC 1 cut(s) 1156
PpsI GAGTC 1 cut(s) 1156
PpuMI RGGWCCY 1 cut(s) 242
PsiI TTATAA 1 cut(s) 855
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 737
PspFI CCCAGC 2 cut(s) 548, 1151
PspGI CCWGG 1 cut(s) 737
PspN4I GGNNCC 4 cut(s) 11, 283, 597, 887
PspPI GGNCC 1 cut(s) 242
PspPPI RGGWCCY 1 cut(s) 242
PsrI GAACNNNNNNTAC 2 cut(s) 726, 758
PstI CTGCAG 1 cut(s) 1042
PstNI CAGNNNCTG 1 cut(s) 605
PsuI RGATCY 1 cut(s) 885
PvuI CGATCG 1 cut(s) 1049
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
RseI CAYNNNNRTG 1 cut(s) 1175
SapI GCTCTTC 1 cut(s) 780
SaqAI TTAA 2 cut(s) 579, 1224
SatI GCNGC 4 cut(s) 288, 645, 753, 953
Sau3AI GATC 4 cut(s) 18, 885, 1046, 1179
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 1 cut(s) 1156
ScrFI CCNGG 1 cut(s) 739
SduI GDGCHC 2 cut(s) 120, 253
SfaNI GCATC 4 cut(s) 77, 210, 611, 825
SfcI CTRYAG 3 cut(s) 195, 732, 1038
SinI GGWCC 1 cut(s) 242
SmiMI CAYNNNNRTG 1 cut(s) 1175
SmlI CTYRAG 1 cut(s) 1236
SmoI CTYRAG 1 cut(s) 1236
Sse9I AATT 4 cut(s) 257, 401, 561, 993
SsiI CCGC 3 cut(s) 100, 285, 827
SspMI CTAG 1 cut(s) 72
StyD4I CCNGG 1 cut(s) 737
StyI CCWWGG 2 cut(s) 147, 541
TaaI ACNGT 5 cut(s) 187, 677, 949, 965, 1066
TaqI TCGA 1 cut(s) 1045
TasI AATT 4 cut(s) 257, 401, 561, 993
TatI WGTACW 1 cut(s) 342
TfiI GAWTC 1 cut(s) 728
Tru1I TTAA 2 cut(s) 579, 1224
Tru9I TTAA 2 cut(s) 579, 1224
TscAI CASTG 3 cut(s) 190, 1098, 1144
TseFI GTSAC 1 cut(s) 1087
TseI GCWGC 4 cut(s) 287, 644, 752, 952
Tsp45I GTSAC 1 cut(s) 1087
TspDTI ATGAA 2 cut(s) 734, 923
TspGWI ACGGA 1 cut(s) 252
TspRI CASTG 3 cut(s) 190, 1098, 1144
VpaK11BI GGWCC 1 cut(s) 242
XapI RAATTY 1 cut(s) 401
XceI RCATGY 1 cut(s) 851
XmnI GAANNNNTTC 1 cut(s) 131
XspI CTAG 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.