Rorug01G0415100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005716
Physical Location & Seq
Forward (+)
51858117 .. 51861813
3697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0415100.1

Sequence Viewer

Length: 3174 bp
ATGAAGAGGACCATGAGAGCCTTCTTAATTCTCAGGTTGCTAACTATTGCAACCATTACTACTAGTATTGGTTCATGCAATGGAAACCTGGGTGTGCCTTGTAAACAAAGCGAAAAACAAGCGCTTCTCATTTTCAAGCAAGATCTTATAGATCCTTCAAATCGGCTTTCATCATGGGTTGCTGAAGAACATTCCAACTGCTGCAATTGGGCTGGAGTTGTCTGTCATTACTCCACCGGCCACATCCATGAGCTGCATCTCGATAACCCCGACTATTTGGTGGATTCCAACTCTTCCTTGGGCGGTAAGATAAATCCTTCCCTGCTCAATTTAACGCATCTCACCTACTTTAACCTAAGTAACAATAACTTTCAAGGGACAAAGATTCCTGGCTTCTTTGGTTCTCTAAATAGTTTAACTCATCTTGACCTCTCACAAGCAAGCTTCGGAGGAATGGTTCCTCATCAATTGGGAAATCTTTCCAGTCTAAGCCATCTCTTTCTTGGTGGCGACGGCCAGAAAGTTGAGAACATGCAATGGATTTCTGGTCTTTCTCAATTGGAACACCTGGACATGAGTGAAGTTGATCTTAGCAAAGCATCTGATCATTGGCTGCTAGTGACAAACATGCTCCCCTCTTTGGTAGAATTAAGCATGAGCGGTTGTCAACTTCATAGCATTCCACTTCTACCTATCATCAATTTTACTTCTCTTGCCATCCTTGATCTTTCTTGGAACAGATTTAATTCTTTGATGCCCGGGTGGGTTTTTAGTCTTAGAAATCTAGTTTCTCTTCATTTGGAGGGTTGCAAGTTCCAAGGTCCAATTCCCAGCAGTCCACACAATATTACGTCTCTCAGGGAAATTGATCTGTTCATGAATGATTTTAGTCGTTCGATACCTGAGTGGTTGTTTAACCACAAAGATCTCACTCTTTTGGAACTGGGATTCAATGCACTTGAAGGGTCACTGCCAGATGGTATGGCGAATATGACTGGTCTTAAAATTCTTAATCTCGATGGGAACCGATTCAATTCAACCATACCTGAGTGGTTGTATAGTTTTAACTATCTTGAGTCCTTGAGTCTATCTGTCAATAACTTTCAGGGTGAAATTTCAAGTTCCATTGGGAACTTGACATCCATCATCAGCCTAGAGTTGGGTTTAAATCTGTTGGAAGGGAAGATCCCAAAGTCATTGGGAAATCTTTGTAAATTGATGGTTCTTGATCTTTCATGGAACCATATCACGGTTGGAAGGTTTTCAGAAATATTTGAAACTTTGTCTGCGTGTGGTTCAAATAGAATCAAGTCATTGTCTTTCAGTTCCTGTAATATTTCTGGTCAGTTAACAGAGCAGGTAGGAACCTTTACAAACTTGAGCTCCCTTGATCTTTCTAATAATTCAATATCAGGTCCCATTCCAGTGTCCCTGGCTAATCTGTCACGCTTAGAAACTTTAGACATCTCTCGGAATCAGTTCAATGGAACTGTTCCAGAAAGCATTGGCCAACTCAAAATGCTAACGATTTTGGATATGTCCTATAATTCATTGGAAGGTGCAGTCTCTGAAGTTCATTTTTCTTGTCTGACAAGATTGTTAGACATCAATGGAAACAAAAATTCATTGATGCTCAATACTAGTCGAGATTGGATTCCTCCTTTTCAACTTCAGTACCTAAGATTAGATTCTTGGCATATAGGGCCTGAATTTCCTACGTGGATTCAGCGACAAGTCCAGTTAAAGGGTGTAAGCTTATCTAGTACAGGACTTTCGGGTACCATTCCAAATTGGGTTTGGCACTCATCTTTAGGTTCTCTTGATCTCTCTCACAATCAATTGCATGGAGAGATCCAAAGTTTAGTTGCTTCCCAGTTGTCAATAATTGACTTAAGTTCTAACCAGTTCAACGGTTCATTGCCTCTTGTTTCTTCCAATGTAAGCATACTGGATCTTTCCAATTCATCATTTTCTGGATCTGTCACCCATTTCTTTTGTGAAGGACCAAATAAGAAACTTGAACTTCTTTACCTTGGGAACAATCATCTCGTTGGGAGAATTCCTAATTGTTGGATGAATTGGAAAAGCCTGAAATTTGTGAATTTGGAAAGCAACAAGTTGACTGGGAATATTCCAAGCTCCATGGGATACTTGCAAGGCCTCATATCACTTCACTTGCGCAATAATCAGTTATCTGGGGAGTTACCGCTATCATTGCAGAAGTGTACTAAATTGCGGGTTGTTGACCTTGGTCTAAATAAGTTTGAGGGAAGCTTACCAACATGGATGGGAAAAAGCCTTCCAAACTTGGGGGTTCTTAACCTTCGTTCAAATAAGCTTCAGGGTGACATTCCACATGAACTCTGCAATCTCACAAGTCTCCAAATCTTGGACATTGCCCATAACAATCTCTCCGGAACAATACCAAGATGTTTCAACAGTCTTGCCATGAATACAGTGTCAAAATCATTTTTCTTAGTTTTAAGTACACTTCGCCAAGATACTTTTGATTGGTGGGTTAAAGAATTTACTTTTGGAGGAAAATATAGCGATAATGCGGTATTGGTGACGAAAGGGAGAGAAGTGGAATACAGCAGCATCCTTGGATTAGTAACAAGCATTGACCTCTCCAATAACATTATATCTGGGGGGATTCCTGAGGAACTGACCAGCCTCTTTGGCTTGCACACATTGAACTTATCAAACAATCTTTTGACCGGAAGAATCCCTTCAAAGATTGGTAATTTGGGTCAGTTAGAATCTCTTGATTTGTCGAAGAACCATCTTTTTGGTGAAATTCCTAAAAGCATGACCAAAATGACATTTCTGAGTCGCTTGAACTTGTCATACAACAATTTGACGGGACGGATTCCAGAAAGCACTCAGCTTCAGACCCTTGATCAATCCAGCTTTGTTGGCAATGAACTTTGTGGTCCTCCACTCATCAAGAATTGCAGTGCAAGCAAGGTGACACCACCAACAGTTGAGCAACAGAGAGGATATGATTTACTTGACGACAAGTGGTTCTACCTGAGCTTGGGATTGGGATTCGCGGTTGGTTTTTGGAGTATACTTGGCTCCTTGCTCATTAACATGCCATGGAGCTTTGCTTTTTCGCAATTCCTCAATAGCATTGTGCTTAAAATTTATGCTTTAATTGTTGAGTATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1057

Amino Acids

116.86

Weight (kDa)

6.06

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 75 6.5e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 107 - 198 5.9e-07 Leucine-rich repeat region
LRR_14 PF23598 217 - 431 3.4e-10 Leucine-rich repeat region
LRR_8 PF13855 437 - 494 6.9e-06 Leucine rich repeat
LRR_14 PF23598 439 - 578 5.5e-09 Leucine-rich repeat region
LRR_8 PF13855 482 - 540 2.7e-06 Leucine rich repeat
LRR_14 PF23598 696 - 803 1.2e-06 Leucine-rich repeat region
LRR_8 PF13855 744 - 804 2e-07 Leucine rich repeat
LRR_14 PF23598 887 - 970 4.4e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2180
Acc36I ACCTGC 1 cut(s) 1348
Acc65I GGTACC 1 cut(s) 1778
AccB1I GGYRCC 1 cut(s) 1778
AccB7I CCANNNNNTGG 1 cut(s) 2389
AccBSI CCGCTC 1 cut(s) 660
AccI GTMKAC 1 cut(s) 3070
AccII CGCG 1 cut(s) 3053
AccIII TCCGGA 1 cut(s) 2414
AciI CCGC 6 cut(s) 303, 660, 2207, 2236, 2558, 3053
AclWI GGATC 5 cut(s) 146, 1180, 1846, 1959, 1984
AcoI YGGCCR 3 cut(s) 238, 514, 1507
AcuI CTGAAG 5 cut(s) 204, 1590, 1655, 2324, 2873
AfaI GTAC 5 cut(s) 1676, 1765, 1780, 2227, 2488
AfeI AGCGCT 1 cut(s) 123
AfiI CCNNNNNNNGG 7 cut(s) 640, 1159, 1249, 1744, 2309, 2389, 3037
AflII CTTAAG 1 cut(s) 1891
AhlI ACTAGT 2 cut(s) 62, 1640
AjnI CCWGG 4 cut(s) 87, 388, 567, 1431
AjuI GAANNNNNNNTTGG 4 cut(s) 810, 842, 2517, 2549
AloI GAACNNNNNNTCC 4 cut(s) 441, 473, 1124, 1156
Alw21I GWGCWC 1 cut(s) 1385
Alw26I GTCTC 3 cut(s) 858, 1570, 2384
AlwI GGATC 5 cut(s) 146, 1180, 1846, 1959, 1984
AlwNI CAGNNNCTG 2 cut(s) 1329, 1568
Ama87I CYCGRG 1 cut(s) 758
Aor13HI TCCGGA 1 cut(s) 2414
Aor51HI AGCGCT 1 cut(s) 123
AoxI GGCC 5 cut(s) 238, 514, 1507, 1703, 2158
ApeKI GCWGC 4 cut(s) 201, 253, 613, 2595
Asp700I GAANNNNTTC 5 cut(s) 478, 1028, 1261, 1478, 2728
Asp718I GGTACC 1 cut(s) 1778
AspLEI GCGC 2 cut(s) 124, 2181
AspS9I GGNCC 6 cut(s) 9, 821, 1415, 1703, 2003, 2933
AsuC2I CCSGG 2 cut(s) 759, 760
AsuHPI GGTGA 7 cut(s) 334, 1121, 1975, 2357, 2578, 2804, 2980
AvaI CYCGRG 1 cut(s) 758
AvaII GGWCC 5 cut(s) 9, 821, 1415, 2003, 2933
AxyI CCTNAGG 1 cut(s) 2658
BaeI ACNNNNGTAYC 4 cut(s) 890, 923, 1658, 1691
BalI TGGCCA 1 cut(s) 1509
BanI GGYRCC 1 cut(s) 1778
BanII GRGCYC 1 cut(s) 1385
Bbv12I GWGCWC 1 cut(s) 1385
BbvI GCAGC 4 cut(s) 188, 240, 600, 2607
BccI CCATC 8 cut(s) 501, 725, 971, 1013, 1151, 1213, 2281, 2790
BceAI ACGGC 1 cut(s) 529
BciT130I CCWGG 4 cut(s) 89, 390, 569, 1433
BciVI GTATCC 1 cut(s) 2141
BclI TGATCA 2 cut(s) 604, 2899
BcnI CCSGG 2 cut(s) 759, 760
BcoDI GTCTC 3 cut(s) 858, 1570, 2384
BcuI ACTAGT 2 cut(s) 62, 1640
BfaI CTAG 6 cut(s) 63, 617, 785, 1154, 1641, 1761
BfoI RGCGCY 1 cut(s) 125
BfrI CTTAAG 1 cut(s) 1891
BfuAI ACCTGC 1 cut(s) 1348
BfuI GTATCC 1 cut(s) 2141
BglI GCCNNNNNGGC 1 cut(s) 2679
BglII AGATCT 2 cut(s) 142, 925
BisI GCNGC 4 cut(s) 202, 254, 614, 2596
BlsI GCNGC 4 cut(s) 203, 255, 615, 2597
Bme1390I CCNGG 6 cut(s) 89, 390, 569, 759, 760, 1433
Bme18I GGWCC 5 cut(s) 9, 821, 1415, 2003, 2933
BmeT110I CYCGRG 1 cut(s) 758
BmgT120I GGNCC 6 cut(s) 9, 821, 1415, 1703, 2003, 2933
BmiI GGNNCC 7 cut(s) 459, 1025, 1241, 1366, 1417, 1780, 3079
BmrFI CCNGG 6 cut(s) 89, 390, 569, 759, 760, 1433
BmrI ACTGGG 3 cut(s) 953, 1867, 2133
BmsI GCATC 6 cut(s) 265, 346, 608, 744, 1620, 2607
BmuI ACTGGG 3 cut(s) 953, 1867, 2133
BoxI GACNNNNGTC 1 cut(s) 2250
BpmI CTGGAG 1 cut(s) 234
Bpu10I CCTNAGC 1 cut(s) 3032
BpuEI CTTGAG 3 cut(s) 1094, 1102, 1399
BpuMI CCSGG 2 cut(s) 759, 760
BsaAI YACGTR 1 cut(s) 1719
BsaWI WCCGGW 2 cut(s) 2414, 2717
BsaXI ACNNNNNCTCC 6 cut(s) 441, 471, 1367, 1397, 2396, 2426
Bsc4I CCNNNNNNNGG 7 cut(s) 640, 1159, 1249, 1744, 2309, 2389, 3037
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 9 cut(s) 483, 948, 1000, 1424, 1738, 1873, 1903, 1953, 2128
Bse21I CCTNAGG 1 cut(s) 2658
Bse3DI GCAATG 6 cut(s) 85, 542, 1916, 2213, 2394, 2926
BseAI TCCGGA 1 cut(s) 2414
BseBI CCWGG 4 cut(s) 89, 390, 569, 1433
BseGI GGATG 6 cut(s) 243, 717, 1139, 2079, 2292, 2598
BseLI CCNNNNNNNGG 7 cut(s) 640, 1159, 1249, 1744, 2309, 2389, 3037
BseMI GCAATG 6 cut(s) 85, 542, 1916, 2213, 2394, 2926
BseMII CTCAG 8 cut(s) 46, 871, 894, 1038, 2649, 2819, 2897, 3023
BseNI ACTGG 9 cut(s) 483, 948, 1000, 1424, 1738, 1873, 1903, 1953, 2128
BseXI GCAGC 4 cut(s) 188, 240, 600, 2607
BseYI CCCAGC 1 cut(s) 830
BsgI GTGCAG 1 cut(s) 1581
Bsh1236I CGCG 1 cut(s) 3053
BshFI GGCC 5 cut(s) 240, 516, 1509, 1705, 2160
BshNI GGYRCC 1 cut(s) 1778
BsiHKAI GWGCWC 1 cut(s) 1385
BsiHKCI CYCGRG 1 cut(s) 758
BsiSI CCGG 4 cut(s) 237, 759, 2415, 2718
BslFI GGGAC 4 cut(s) 391, 1401, 1414, 2877
BslI CCNNNNNNNGG 7 cut(s) 640, 1159, 1249, 1744, 2309, 2389, 3037
BsmAI GTCTC 3 cut(s) 858, 1570, 2384
BsmBI CGTCTC 1 cut(s) 858
BsmFI GGGAC 4 cut(s) 391, 1401, 1414, 2877
BsmI GAATGC 1 cut(s) 678
BsnI GGCC 5 cut(s) 240, 516, 1509, 1705, 2160
BsoBI CYCGRG 1 cut(s) 758
Bsp1286I GDGCHC 1 cut(s) 1385
Bsp13I TCCGGA 1 cut(s) 2414
Bsp19I CCATGG 2 cut(s) 2142, 3098
BspACI CCGC 6 cut(s) 303, 660, 2207, 2236, 2558, 3053
BspANI GGCC 5 cut(s) 240, 516, 1509, 1705, 2160
BspCNI CTCAG 8 cut(s) 45, 870, 895, 1039, 2650, 2820, 2896, 3024
BspEI TCCGGA 1 cut(s) 2414
BspFNI CGCG 1 cut(s) 3053
BspHI TCATGA 1 cut(s) 876
BspLI GGNNCC 7 cut(s) 459, 1025, 1241, 1366, 1417, 1780, 3079
BspMI ACCTGC 1 cut(s) 1348
BspPI GGATC 5 cut(s) 146, 1180, 1846, 1959, 1984
BspT107I GGYRCC 1 cut(s) 1778
BspTI CTTAAG 1 cut(s) 1891
BsrBI CCGCTC 1 cut(s) 660
BsrDI GCAATG 6 cut(s) 85, 542, 1916, 2213, 2394, 2926
BsrFI RCCGGY 1 cut(s) 236
BsrI ACTGG 9 cut(s) 483, 948, 1000, 1424, 1738, 1873, 1903, 1953, 2128
BssAI RCCGGY 1 cut(s) 236
BssNAI GTATAC 1 cut(s) 3071
BssT1I CCWWGG 7 cut(s) 297, 817, 2032, 2142, 2248, 2602, 3098
Bst1107I GTATAC 1 cut(s) 3071
Bst2UI CCWGG 4 cut(s) 89, 390, 569, 1433
Bst4CI ACNGT 6 cut(s) 1252, 1492, 1913, 2441, 2458, 2983
Bst6I CTCTTC 2 cut(s) 298, 798
BstAFI CTTAAG 1 cut(s) 1891
BstBAI YACGTR 1 cut(s) 1719
BstC8I GCNNGC 3 cut(s) 442, 2684, 2962
BstDSI CCRYGG 2 cut(s) 2142, 3098
BstF5I GGATG 6 cut(s) 243, 717, 1139, 2079, 2292, 2598
BstFNI CGCG 1 cut(s) 3053
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 2 cut(s) 124, 2181
BstMAI GTCTC 3 cut(s) 858, 1570, 2384
BstMWI GCNNNNNNNGC 5 cut(s) 1702, 2215, 2679, 2916, 2961
BstNI CCWGG 4 cut(s) 89, 390, 569, 1433
BstNSI RCATGY 3 cut(s) 535, 631, 3097
BstPAI GACNNNNGTC 1 cut(s) 2250
BstSCI CCNGG 6 cut(s) 87, 388, 567, 757, 758, 1431
BstUI CGCG 1 cut(s) 3053
BstV1I GCAGC 4 cut(s) 188, 240, 600, 2607
BstX2I RGATCY 7 cut(s) 142, 151, 925, 1185, 1851, 1951, 1976
BstXI CCANNNNNNTGG 1 cut(s) 2789
BstYI RGATCY 7 cut(s) 142, 151, 925, 1185, 1851, 1951, 1976
BstZ17I GTATAC 1 cut(s) 3071
Bsu36I CCTNAGG 1 cut(s) 2658
BsuI GTATCC 1 cut(s) 2141
BsuRI GGCC 5 cut(s) 240, 516, 1509, 1705, 2160
BtgI CCRYGG 2 cut(s) 2142, 3098
BtsCI GGATG 6 cut(s) 243, 717, 1139, 2079, 2292, 2598
BtsI GCAGTG 2 cut(s) 968, 2962
BtsIMutI CAGTG 4 cut(s) 968, 1431, 2463, 2962
BveI ACCTGC 1 cut(s) 1348
Cac8I GCNNGC 3 cut(s) 442, 2684, 2962
CaiI CAGNNNCTG 2 cut(s) 1329, 1568
CciI TCATGA 1 cut(s) 876
CfoI GCGC 2 cut(s) 124, 2181
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 6 cut(s) 9, 821, 1415, 1703, 2003, 2933
Cfr9I CCCGGG 1 cut(s) 758
Csp6I GTAC 5 cut(s) 1675, 1764, 1779, 2226, 2487
CviQI GTAC 5 cut(s) 1675, 1764, 1779, 2226, 2487
DraI TTTAAA 1 cut(s) 1167
EaeI YGGCCR 3 cut(s) 238, 514, 1507
Eam1104I CTCTTC 2 cut(s) 298, 798
EarI CTCTTC 2 cut(s) 298, 798
Ecl136II GAGCTC 1 cut(s) 1383
Eco130I CCWWGG 7 cut(s) 297, 817, 2032, 2142, 2248, 2602, 3098
Eco147I AGGCCT 1 cut(s) 2160
Eco24I GRGCYC 1 cut(s) 1385
Eco47I GGWCC 5 cut(s) 9, 821, 1415, 2003, 2933
Eco47III AGCGCT 1 cut(s) 123
Eco53kI GAGCTC 1 cut(s) 1383
Eco57I CTGAAG 5 cut(s) 204, 1590, 1655, 2324, 2873
Eco81I CCTNAGG 1 cut(s) 2658
Eco88I CYCGRG 1 cut(s) 758
EcoICRI GAGCTC 1 cut(s) 1383
EcoO109I RGGNCCY 2 cut(s) 1415, 1703
EcoRI GAATTC 1 cut(s) 2058
EcoRII CCWGG 4 cut(s) 87, 388, 567, 1431
EcoT14I CCWWGG 7 cut(s) 297, 817, 2032, 2142, 2248, 2602, 3098
EcoT38I GRGCYC 1 cut(s) 1385
ErhI CCWWGG 7 cut(s) 297, 817, 2032, 2142, 2248, 2602, 3098
Esp3I CGTCTC 1 cut(s) 858
FalI AAGNNNNNCTT 4 cut(s) 573, 605, 2713, 2745
FaqI GGGAC 4 cut(s) 391, 1401, 1414, 2877
FauI CCCGC 1 cut(s) 2229
FbaI TGATCA 2 cut(s) 604, 2899
FblI GTMKAC 1 cut(s) 3070
Fnu4HI GCNGC 4 cut(s) 202, 254, 614, 2596
FokI GGATG 6 cut(s) 230, 704, 1126, 2086, 2299, 2585
FriOI GRGCYC 1 cut(s) 1385
Fsp4HI GCNGC 4 cut(s) 202, 254, 614, 2596
FspBI CTAG 6 cut(s) 63, 617, 785, 1154, 1641, 1761
FspI TGCGCA 1 cut(s) 2180
GlaI GCGC 2 cut(s) 123, 2180
GluI GCNGC 4 cut(s) 202, 254, 614, 2596
GsaI CCCAGC 1 cut(s) 834
GsuI CTGGAG 1 cut(s) 234
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 5 cut(s) 240, 516, 1509, 1705, 2160
HapII CCGG 4 cut(s) 237, 759, 2415, 2718
HhaI GCGC 2 cut(s) 124, 2181
Hin6I GCGC 2 cut(s) 122, 2179
HinP1I GCGC 2 cut(s) 122, 2179
HincII GTYRAC 4 cut(s) 668, 1350, 2121, 2245
HindII GTYRAC 4 cut(s) 668, 1350, 2121, 2245
HindIII AAGCTT 4 cut(s) 442, 1753, 2272, 2336
HpaI GTTAAC 1 cut(s) 1350
HpaII CCGG 4 cut(s) 237, 759, 2415, 2718
HphI GGTGA 7 cut(s) 334, 1121, 1975, 2357, 2578, 2804, 2980
Hpy166II GTNNAC 9 cut(s) 104, 668, 839, 1350, 2121, 2226, 2245, 2489, 3071
Hpy188I TCNGA 8 cut(s) 449, 604, 1267, 1473, 1570, 1590, 2829, 2892
Hpy8I GTNNAC 9 cut(s) 104, 668, 839, 1350, 2121, 2226, 2245, 2489, 3071
Hpy99I CGWCG 1 cut(s) 515
HpyCH4III ACNGT 6 cut(s) 1252, 1492, 1913, 2441, 2458, 2983
HpyCH4IV ACGT 2 cut(s) 851, 1718
HpyF10VI GCNNNNNNNGC 5 cut(s) 1702, 2215, 2679, 2916, 2961
HpySE526I ACGT 2 cut(s) 851, 1718
HspAI GCGC 2 cut(s) 122, 2179
Kpn2I TCCGGA 1 cut(s) 2414
KpnI GGTACC 1 cut(s) 1782
Ksp22I TGATCA 2 cut(s) 604, 2899
KspAI GTTAAC 1 cut(s) 1350
LmnI GCTCC 5 cut(s) 636, 1388, 2144, 3083, 3102
Lsp1109I GCAGC 4 cut(s) 188, 240, 600, 2607
LweI GCATC 6 cut(s) 265, 346, 608, 744, 1620, 2607
MaeI CTAG 6 cut(s) 63, 617, 785, 1154, 1641, 1761
MaeII ACGT 2 cut(s) 851, 1718
MbiI CCGCTC 1 cut(s) 660
MboII GAAGA 8 cut(s) 16, 197, 285, 785, 1195, 1923, 2733, 2788
MfeI CAATTG 4 cut(s) 205, 467, 557, 1838
MflI RGATCY 7 cut(s) 142, 151, 925, 1185, 1851, 1951, 1976
MhlI GDGCHC 1 cut(s) 1385
MlsI TGGCCA 1 cut(s) 1509
MluNI TGGCCA 1 cut(s) 1509
MlyI GAGTC 3 cut(s) 1085, 1093, 2839
MmeI TCCRAC 5 cut(s) 219, 312, 1155, 1234, 2051
Mox20I TGGCCA 1 cut(s) 1509
MroI TCCGGA 1 cut(s) 2414
MroXI GAANNNNTTC 5 cut(s) 478, 1028, 1261, 1478, 2728
MscI TGGCCA 1 cut(s) 1509
MslI CAYNNNNRTG 3 cut(s) 246, 1424, 3092
Msp20I TGGCCA 1 cut(s) 1509
MspCI CTTAAG 1 cut(s) 1891
MspI CCGG 4 cut(s) 237, 759, 2415, 2718
MspR9I CCNGG 6 cut(s) 89, 390, 569, 759, 760, 1433
MunI CAATTG 4 cut(s) 205, 467, 557, 1838
Mva1269I GAATGC 1 cut(s) 678
MvaI CCWGG 4 cut(s) 89, 390, 569, 1433
MvnI CGCG 1 cut(s) 3053
MwoI GCNNNNNNNGC 5 cut(s) 1702, 2215, 2679, 2916, 2961
NciI CCSGG 2 cut(s) 759, 760
NcoI CCATGG 2 cut(s) 2142, 3098
NlaIV GGNNCC 7 cut(s) 459, 1025, 1241, 1366, 1417, 1780, 3079
NmuCI GTSAC 7 cut(s) 619, 966, 1443, 1981, 2345, 2566, 2968
NsbI TGCGCA 1 cut(s) 2180
NspI RCATGY 3 cut(s) 535, 631, 3097
PagI TCATGA 1 cut(s) 876
PceI AGGCCT 1 cut(s) 2160
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 1 cut(s) 678
PdmI GAANNNNTTC 5 cut(s) 478, 1028, 1261, 1478, 2728
PflMI CCANNNNNTGG 1 cut(s) 2389
PkrI GCNGC 4 cut(s) 203, 255, 615, 2597
PleI GAGTC 3 cut(s) 1084, 1092, 2838
PpsI GAGTC 3 cut(s) 1084, 1092, 2838
Ppu21I YACGTR 1 cut(s) 1719
PpuMI RGGWCCY 1 cut(s) 1415
PshAI GACNNNNGTC 1 cut(s) 2250
Psp124BI GAGCTC 1 cut(s) 1385
Psp5II RGGWCCY 1 cut(s) 1415
Psp6I CCWGG 4 cut(s) 87, 388, 567, 1431
PspFI CCCAGC 1 cut(s) 830
PspGI CCWGG 4 cut(s) 87, 388, 567, 1431
PspN4I GGNNCC 7 cut(s) 459, 1025, 1241, 1366, 1417, 1780, 3079
PspPI GGNCC 6 cut(s) 9, 821, 1415, 1703, 2003, 2933
PspPPI RGGWCCY 1 cut(s) 1415
PsrI GAACNNNNNNTAC 2 cut(s) 2831, 2863
PstNI CAGNNNCTG 2 cut(s) 1329, 1568
PsuI RGATCY 7 cut(s) 142, 151, 925, 1185, 1851, 1951, 1976
RsaI GTAC 5 cut(s) 1676, 1765, 1780, 2227, 2488
RsaNI GTAC 5 cut(s) 1675, 1764, 1779, 2226, 2487
RseI CAYNNNNRTG 3 cut(s) 246, 1424, 3092
SacI GAGCTC 1 cut(s) 1385
SatI GCNGC 4 cut(s) 202, 254, 614, 2596
Sau96I GGNCC 6 cut(s) 9, 821, 1415, 1703, 2003, 2933
SchI GAGTC 3 cut(s) 1085, 1093, 2839
ScrFI CCNGG 6 cut(s) 89, 390, 569, 759, 760, 1433
SduI GDGCHC 1 cut(s) 1385
SfaNI GCATC 6 cut(s) 265, 346, 608, 744, 1620, 2607
SinI GGWCC 5 cut(s) 9, 821, 1415, 2003, 2933
SmaI CCCGGG 1 cut(s) 760
SmiMI CAYNNNNRTG 3 cut(s) 246, 1424, 3092
SmlI CTYRAG 4 cut(s) 1073, 1081, 1378, 1891
SmoI CTYRAG 4 cut(s) 1073, 1081, 1378, 1891
SpeI ACTAGT 2 cut(s) 62, 1640
SseBI AGGCCT 1 cut(s) 2160
SsiI CCGC 6 cut(s) 303, 660, 2207, 2236, 2558, 3053
SspI AATATT 4 cut(s) 847, 1272, 1336, 2131
SspMI CTAG 6 cut(s) 63, 617, 785, 1154, 1641, 1761
SstI GAGCTC 1 cut(s) 1385
StuI AGGCCT 1 cut(s) 2160
StyD4I CCNGG 6 cut(s) 87, 388, 567, 757, 758, 1431
StyI CCWWGG 7 cut(s) 297, 817, 2032, 2142, 2248, 2602, 3098
TaaI ACNGT 6 cut(s) 1252, 1492, 1913, 2441, 2458, 2983
TaiI ACGT 2 cut(s) 854, 1721
TaqI TCGA 5 cut(s) 261, 896, 1017, 1645, 2774
TatI WGTACW 3 cut(s) 1763, 2225, 2486
TscAI CASTG 4 cut(s) 975, 1431, 2463, 2962
TseFI GTSAC 7 cut(s) 619, 966, 1443, 1981, 2345, 2566, 2968
TseI GCWGC 4 cut(s) 201, 253, 613, 2595
Tsp45I GTSAC 7 cut(s) 619, 966, 1443, 1981, 2345, 2566, 2968
TspGWI ACGGA 1 cut(s) 2881
TspMI CCCGGG 1 cut(s) 758
TspRI CASTG 4 cut(s) 975, 1431, 2463, 2962
Van91I CCANNNNNTGG 1 cut(s) 2389
Vha464I CTTAAG 1 cut(s) 1891
VpaK11BI GGWCC 5 cut(s) 9, 821, 1415, 2003, 2933
XceI RCATGY 3 cut(s) 535, 631, 3097
XcmI CCANNNNNNNNNTGG 4 cut(s) 295, 500, 1250, 1794
XmaI CCCGGG 1 cut(s) 758
XmiI GTMKAC 1 cut(s) 3070
XmnI GAANNNNTTC 5 cut(s) 478, 1028, 1261, 1478, 2728
XspI CTAG 6 cut(s) 63, 617, 785, 1154, 1641, 1761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.