Rorug01G0480900

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002916
Physical Location & Seq
Reverse (-)
56873698 .. 56888965
15268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0480900.1

Sequence Viewer

Length: 6042 bp
ATGTCGTCGGAGGAAGAGAAGCTGTTGAAGGAGGCGAAGAAATTGCCGTGGGATGATCGGCTTTTCCATAAGAACTGGAAGGTGCGGAATGAAGCCAACATCGATTTGGCTGCCCTATGCGACTCCATCACCGATCCAAAGGACTCTCGTCTCCGTGAATTTGGTTCGTTTTTTAGGAAGACTCTGGCGGATGCCAATTCGCCGGTACAAGAGAAGGCACTTGATGCATTGATTGCTTATTTACGAGCGGCTGATGCTGATGCTGGAAGGTATGGCAAGGAAGTTTGCGATACCATCGTTGCGAAATGCCTCACCGGCAGGCCGAAGACGGTGGAGAAGGCTCAGGCTTCCTTTATGCTTTGGGTCGAATTGGAGGCTGTGGACGCTTTCTTGGATGCCATGGAGAAAGCAATAAAAGCCAAAGTTGCCAAAGCTGTTGTGCCTGCAATTGATGTCATGTTTCAAGCCTTAAGTGAATTTGGCTCAAAAATTATTCCTCCAAAAAGGATTTTAAAGATGCTTCCTGAACTATTTGACCATCAAGATCAAAATGTTCGTGCATCTTCTAAAGGACTTACTCTTGAGCTTTGCCGTTGGATAGGAAAAGACCCTGTAAAATCCATATTGTTTGAAAAAATGAGGGATACGATGAAAAAAGAATTGGAGTCTGAGCTCGTCAATGTTACCGGGACAGCCAGGCCATCTCGCAAAATCAGATCTGAGCAAGACAAGGAGCCAGAAAGGGAAGTTGTATCCGAAGTTGTTGGTCCTGGTCTTTCTGAGGAATCTACAGTGGATGCTCCTCAGGAAATAGATGAATATGAGCTTGTTGATCCTGTTGATATATTGACTCCTCTGGATAAGTCTGGATTTTGGGATGGAGTGAAAGCATCCAAATGGTCAGAGCGAAAGGAGGCTGTTGCTGAGCTCACCAAGCTAGCTTCAACCAAAAGAATAGCTCCTGGTGACTTCACCGAAATATGTAGGACATTAAAGAAGCTTATAACAGATGTGAACATAGCTGTTGCAGTTGAAGCTATCCAAGCTCTCGGCAATCTTGCCAAGGGCCTAAGAACCCACTTCTCTGGGAGTTCGCGTTTTCTATTGCCAGGGTTACTTGAGAAATTGAAAGAGAAAAAGCCAACTATGAGTGAGGCGCTTACCCAGACTCTTCAAGCTATGCATACAGCTGGCTGCTTAAATCTTGTTGACATTGTTGAAGATGTTAAGACAGCAGTTAAAAATAAAGTACCTCTTGTACGATCATCGACTTTGAACTGGGTGACATTCTGTATTGAAACAAGTAACAAGGCTGTTGTTTTAAAGTTACACAAGGATTATGTTCCTATATTTATGGAGTGCCTCAATGATGGAACCCCAGAAGTGAGGGATGCAGCCTTTTCAGCTCTGACAGCAGTAGCTAAGTTGGTTGGTATGAGGCCTTTGGAGAGGTCCCTGGAGAAACTTGATGATGTCAGAAGAAAGAAGCTTTCTGAAATGATTATGGGTTCCGAGGGTGGTACATCCACTGCTGCAAGCGCAGTTCAAAGCTCTGGTGTCACTGCAGCCTCTCTGGAGACTTCAGACAGTTCATTTGTTCGAAAGTCAGCTGCAAGCATGCTTAGTGGGAAGAGGCCTGTTCAGGCAGTTCCTGCTAAACAAAAAGTGGGATCTGGAAAATCTGGTGGCAGTAAGAAAGGGGAAGTAACTGTACAGGCAAAAGCTTCCAAGTCAGTTGAACCACCTGAAGACGTTGAGCCAGCAGAAATGAGTCTTGAAGAAATTGAAAGCAGATTAGGTTCTCTTATAAAAGTGGATACCATCTCTCAATTGAAGAGCACTGTGTGGAAAGAACGGCTTGAAGCCATATCCTCATTTAAACAAGAAGTGGAAGCTTTGCAGGACATCAACCAGTCTGTTGAGTTATTAATTCGTTTACTTTGCGCTGTCCCTGGTTGGAGTGAAAAAAATGTTCAGGTTCAACAACAGGTAATTGAAGTTATTACCTACATAGCCTCCACTGCAATGAAATTTCCGAAGAAATGTGTGGTACTTTGTCTACTGGGTATAAGCGAGCGGGTAGCAGATATCAAGACTCGGACTCATGCCATGAAGTGTCTGACTTCTTTCTCTGAAGCCATAGGTCCAGGATTTATTTTTGAAAGACTTTACAAAATAATGAAAGAGCACAAGAACCCGAAGGTTCTTAGCGAGGGAATATTGTGGATGGTTTCAGCTGTTGAAGATTTTGGTGTCTCACATTTGAAGCTTAAGGATTTGATTGATTTTTGCAAAGATACTGGACTGCAGTCCAGTGCTGCTGCTACTAGAAATTCTACTATAAAACTTTTGGGTGTTACGCATAAGTTTGTTGGTCCAGATATTAAAGGATTTCTTACGGATGTCAAACCTGCACTGCTTAGTGCACTGGAAGCAGAGTATGAAAAAAATCCATTTGAGGGTGCTGCAGTGGTTCTGAAGAGAAATGTTCGAGCAACAGAATCTACTTCATCAATTTCTGCTAGTGGGTTGGACAGTCTTCCACGTGAAGATATTAGTGGAAAAATTACACCTACTCTGCTGAAGAATTTGGAGAGCCCTGATTGGAAGGTTCGCTTGGAGTCAATTGATGCTGTAAATAAAATTTTAGAAGAGGCTAATAAGCGCATCCAGCCAACTGGAACTGTGGAGTTATTTGGTGCCTTAAGGGGACGATTATATGACAGCAATAAGAACCTAGTTGCAGCAACCTTGACAGCTATTGGTAATGTTGCATCTGCAATGGGAGCACCGGTTGAAAAGGCGAGCAAGGGCATTCTGTCAGATATTTTGAAATGTATTGGAGACAATAAGAAGCATATGAGAGAATGCACTTTAGCTACTTTAGACTCATGGCTCTCTGCTGTGAATCTTGATAAAATGGTTCCTTATATCACGGCAGCTATAACAGAAACCAAGCTTGGTGCAGAAGGGCGCAAAGATCTTTTTGACTGGTTGACGAGGCAGCTTTCTGTGCTAAGTGATTATAATGATGCTGTACATCTTCTGAAACCAGCTTCCTCTGCTATGACGGATAAATCTTCAGATGTTCGTAAAGCAGCAGAGGCATGCATTGCTGAAATTTTGAGAGTTAGTGGACATGAAGCGGTTGAGAAAATTTTGAAAGATATTCAAGGGCCAGCTTTAGCTCTTGTTCTTGAACGGTTGAAGCCTTCCGGATCTTTACAAGCAATATCAACGGGACCAACATCCAAAAGCATCCCAAAGGTTGGAAAATCTGCTTCCAATGGAGTGTTAAAGCCTGGAACGAAATCCATATCTTCAAGGACCATTGCAATGAAGGGATCAAGGCCAGGTTCAATACTGTCTGTCCAAGATATAGCTGTCCAGTCACAGGCCTTGCTAAATGTTAAAGATTCAGTTAAGGTGGATAGGGAGAGAATAGTGGTTAAGAGGTTTAAGTTTGAAGAGCCACGAATAGAACAGATTCAAGATCTTGAGAATGATATGATGAAGTACTTCAGAGAGGATCTGCATAGGCGGCTTCTTAGTACAGATTTTAAGAAGCAAGTAGATGGGCTTGAGATGCTGCAGAAGGCACTTCCAACTATTAGAAAGGAAATTATTGAAGTTCTGGATATACTGCTTAGGTGGTTTGTTTTACAATTTTGTAAATCAAACACAACATCCCTGTTAAAGGTGCTGGAATTTCTTCATGACCTTTTTGACATGTTTAGGGATGAGGGATACATGTTGACCGAGTCAGAAGCAGCAATTCTTCTTCCATGCCTAATGGAGAAGCTTGGGCACAATGGTTCTGGAGTACGAAAAGAAATGAAGGAGCTGGCCAGACAAATTGTTCAGGCATATACTGCAGCAAAGAGTTTACCTTATATTTTGGAGGGATTGCGTTCTAAGAACTACCGGACACGAATTGAGTGTGCTGAACTTGTTGGATATTTGATCGATCATCATGGAGCTGAGATAAGTGGACAATTAAAATCTTTGCAAATTGTTGCAAGCTTGACTGCAGAAAGAGAGAATGAAATTAGGAAGGCTGCTCTAAATACGCTAGCTTCCGGTTATAAGCTTCTTGGGGAGGACATATGGAGATATGTCGGGAAGCTAACAGGTGCTCAGAAAAGCTTGATAGAAGAGAGATTTAAGTATACGGTTAAAGATATGGAGAAAAACAAGGAAGGAAAGCCTGGTGAAGCTAGGGCTTCTTTAAGGCGTTCTGTCAGGGAAATTGGGTCTGATGTAGCAGAGCAGAGTGGGGAGGTTGCACGATCTGTTGCTGCTCCAACACTTGCTAGGGGCAATTATGGTCATTCTGAGATTCACAGGGAGAGACAGCTGATGCCTCGTGGATTTGCTGCTGTTAATGGCCCAACAGACTGGAATGAAGCTTTGGAGATCATTTCTTCTGAGGATCCTGATCAGTCGGTTGAAGGAATGAAAGTTGTGTGTCATGAGTTGGCACAGTCGACCAGTGATCCAGAAGGCAGTGCAATGGATGACCTAGTGAAGGATGCAGATAAACTTGTTTCGTGCTTAGCAAAGAAGGCAACAACGACTTTTGACTTCAGTTTGACAGGAGCTTCATCTAGGTCCTGTAAATATGTCCTGAACACTCTCATGCAGACATTTCAAAATAAAAGATTTGCACATGCTGTCAAGGAGAGCACTCTTGATAGTCTAATCACTGAGCTCCTGCTCTGGCTTTTAGATGAGAGGGTTCCCCATATGGATGATGGCAGCCAACTTCTCAAAGCCTTGAATGTCTTGATGCTAAAGATTCTGGATAATGCCGACCGCACTTCGTCCTTTGTGGTTCTTATCAATCTCTTACGTCCTTTAGATCCCTCAAGATGGCCTGCACCTCCAGCGAATGAAACGTTGGCTGCCAGAAACCAGAAGTTCTCTGATCTGGTTGTCAAATGTTTAATCAAACTTACAAAGGTTCTTCAAAGCACCATATATGATGTCGATCTGGACCACATCCTTCAAAGTATCCATTTATACCTTCAAGACTTGGGTATGGAAGAAATCCGGAGAAGAGCTGGTGCCGATGACAAACCATTGCGTATGGTGAAAACTGTTCTACATGAGCTTGTGAAGCTTCGCGGAGCTGCAATTAAAGGTCACCTTTCTATGGTTCCTATAGACATGAAGCCCCAGCCAATTATTCTTGCTTACATTGATCTTAATCTTGAGACTCTAGCGGCAGCAAGGATGTTGACATCAACTGGGTCTGGGGGCCAAACTCATTGGGGTGATTCAGCAGCTAATAATCCTTCCTCTGCTACACATTCTGCTGATGCACAATTAAAGCAAGAACTTGCTGCCATTTTCAAGAAGATTGGTGACAAGCAGACTTGCTCCATTGGTCTATATGAACTATATCGTATAACCCAACTCTACCCTAAAGTAGATATATTCTCCCAGCTCCAAAATGCCAGTGAGGCTTTTCGGACGTATATTAGAGATGGTTTAGCACAGATGGAGAAGAATGCAGCAGCTGGAAGGACACCTTCAAGTGTACCAATGCCTACTCCTCCCCCAGCTTCTCTTACCGTTTCTTCTCCTGAATTTGCACCCCTTTCCCCTGTACACACAAATTCCTTAATGGATTCAAAATCATTTAATGTGAAGAGTGAACCAACTAGCTTTAATCTTCCACCGGCATATGCTGACGACAACAGGCTTCACAATGCCAATACTGCTAGAGGCCTGGTTGAAAACTCGCTAGTTGACCCAAGGAATGAGAGATATATGGGTGGAGTGACTAGTGGGACACTAGATGCAATTAGAGAGAGGATGAAAAGCATGCAACTAGCGGCAGCTTCTGGGAACCTGGAGTCAGAAGCTAGGCCATTAATGTATGTAAATGACAACCAAAATCAGGGACTCGCTGGTCAGATAAATCGGGCATCAGAGAATCCCCTCCAAAGTGGGGTTCTCCCTATGGATGAAAGGGCATTATCTGGGCTTCAAGCTCGGATGGAGAGACTAAAAAGTGGGACACTTGAACCCCTTTAA

Protein Analysis

2013

Amino Acids

221.66

Weight (kDa)

8.2

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XMAP215_CLASP_TOG PF21041 12 - 224 4.1e-59 XMAP215/Dis1/CLASP, TOG domain
XMAP215_CLASP_TOG PF21041 298 - 499 1.4e-08 XMAP215/Dis1/CLASP, TOG domain
CLASP_N PF12348 299 - 478 8.5e-10 CLASP N terminal
XMAP215_CLASP_TOG PF21041 609 - 814 7.7e-11 XMAP215/Dis1/CLASP, TOG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1004, 1808, 3027, 4086
AasI GACNNNNNNGTC 1 cut(s) 5916
Acc36I ACCTGC 1 cut(s) 2419
AccB1I GGYRCC 2 cut(s) 2699, 5066
AccB7I CCANNNNNTGG 1 cut(s) 3269
AccBSI CCGCTC 2 cut(s) 248, 2077
AccI GTMKAC 3 cut(s) 2059, 4169, 4487
AccII CGCG 2 cut(s) 1096, 5127
AccIII TCCGGA 2 cut(s) 3215, 5052
AclI AACGTT 1 cut(s) 4898
AcoI YGGCCR 1 cut(s) 3846
AcuI CTGAAG 8 cut(s) 1566, 1767, 2154, 2498, 2603, 3066, 3505, 4570
AcvI CACGTG 1 cut(s) 2546
AdeI CACNNNGTG 1 cut(s) 1845
AfiI CCNNNNNNNGG 9 cut(s) 2459, 3269, 3394, 3697, 4528, 4872, 5155, 5905, 5956
AflII CTTAAG 3 cut(s) 469, 2270, 2704
AflIII ACRYGT 2 cut(s) 3729, 3750
AgeI ACCGGT 1 cut(s) 2791
AhlI ACTAGT 1 cut(s) 5789
Alw21I GWGCWC 9 cut(s) 675, 930, 1841, 2190, 2428, 2791, 4138, 4688, 4713
Alw26I GTCTC 7 cut(s) 155, 1571, 2260, 2838, 4345, 5210, 6004
Alw44I GTGCAC 1 cut(s) 2424
AlwNI CAGNNNCTG 3 cut(s) 1652, 5522, 5849
Aor13HI TCCGGA 2 cut(s) 3215, 5052
ApaLI GTGCAC 1 cut(s) 2424
ArsI GACNNNNNNTTYG 2 cut(s) 1577, 1609
AseI ATTAAT 2 cut(s) 1928, 5879
AsiGI ACCGGT 1 cut(s) 2791
Asp700I GAANNNNTTC 3 cut(s) 3486, 3518, 3711
AspLEI GCGC 5 cut(s) 1159, 1541, 1946, 2667, 2976
AsuC2I CCSGG 1 cut(s) 688
AsuII TTCGAA 1 cut(s) 1600
AsuNHI GCTAGC 2 cut(s) 937, 4072
AvaII GGWCC 8 cut(s) 767, 1452, 2144, 2375, 3242, 3327, 4611, 4996
AxyI CCTNAGG 1 cut(s) 804
BaeGI GKGCMC 2 cut(s) 2428, 3810
BaeI ACNNNNGTAYC 2 cut(s) 282, 315
BalI TGGCCA 1 cut(s) 3848
BamHI GGATCC 1 cut(s) 4432
BanI GGYRCC 2 cut(s) 2699, 5066
BanII GRGCYC 4 cut(s) 675, 930, 2600, 4713
BarI GAAGNNNNNNTAC 2 cut(s) 1695, 1727
BauI CACGAG 1 cut(s) 4365
BbrPI CACGTG 1 cut(s) 2546
BbsI GAAGAC 4 cut(s) 185, 332, 1755, 2531
Bbv12I GWGCWC 9 cut(s) 675, 930, 1841, 2190, 2428, 2791, 4138, 4688, 4713
BceAI ACGGC 4 cut(s) 31, 576, 1871, 2952
BcgI CGANNNNNNTGC 4 cut(s) 25, 59, 92, 126
BciVI GTATCC 5 cut(s) 637, 763, 1810, 3740, 5024
BclI TGATCA 1 cut(s) 4438
BcnI CCSGG 1 cut(s) 688
BcoDI GTCTC 7 cut(s) 155, 1571, 2260, 2838, 4345, 5210, 6004
BcuI ACTAGT 1 cut(s) 5789
BfmI CTRYAG 8 cut(s) 789, 1563, 2306, 2466, 3590, 3873, 4029, 5163
BfoI RGCGCY 1 cut(s) 1160
BfrI CTTAAG 3 cut(s) 469, 2270, 2704
BfuAI ACCTGC 1 cut(s) 2419
BfuI GTATCC 5 cut(s) 637, 763, 1810, 3740, 5024
BglI GCCNNNNNGGC 2 cut(s) 315, 5465
BglII AGATCT 3 cut(s) 716, 2980, 3493
BlpI GCTNAGC 2 cut(s) 924, 4555
BmcAI AGTACT 1 cut(s) 3518
Bme18I GGWCC 8 cut(s) 767, 1452, 2144, 2375, 3242, 3327, 4611, 4996
BmrI ACTGGG 3 cut(s) 1288, 2072, 5259
BmtI GCTAGC 2 cut(s) 941, 4076
BmuI ACTGGG 3 cut(s) 1288, 2072, 5259
BoxI GACNNNNGTC 2 cut(s) 147, 2308
BpiI GAAGAC 4 cut(s) 185, 332, 1755, 2531
BpmI CTGGAG 5 cut(s) 1478, 1595, 3840, 4869, 5879
Bpu10I CCTNAGC 2 cut(s) 342, 3647
Bpu1102I GCTNAGC 2 cut(s) 924, 4555
Bpu14I TTCGAA 1 cut(s) 1600
BpuEI CTTGAG 6 cut(s) 602, 1139, 3518, 3602, 4852, 5234
BpuMI CCSGG 1 cut(s) 688
Bsa29I ATCGAT 2 cut(s) 102, 3966
BsaAI YACGTR 1 cut(s) 2546
BsaBI GATNNNNATC 3 cut(s) 4437, 4989, 5208
BsaJI CCNNGG 8 cut(s) 47, 399, 1062, 1109, 1455, 1512, 1951, 5759
BsaWI WCCGGW 5 cut(s) 2791, 3215, 3924, 4079, 5052
BsaXI ACNNNNNCTCC 6 cut(s) 1081, 1111, 2000, 2030, 2778, 2808
Bsc4I CCNNNNNNNGG 9 cut(s) 2459, 3269, 3394, 3697, 4528, 4872, 5155, 5905, 5956
Bse118I RCCGGY 4 cut(s) 202, 314, 2791, 5683
Bse21I CCTNAGG 1 cut(s) 804
Bse3DI GCAATG 7 cut(s) 2031, 2787, 3111, 3330, 3342, 4518, 5081
Bse8I GATNNNNATC 3 cut(s) 4437, 4989, 5208
BseAI TCCGGA 2 cut(s) 3215, 5052
BseCI ATCGAT 2 cut(s) 102, 3966
BseDI CCNNGG 8 cut(s) 47, 399, 1062, 1109, 1455, 1512, 1951, 5759
BseJI GATNNNNATC 3 cut(s) 4437, 4989, 5208
BseLI CCNNNNNNNGG 9 cut(s) 2459, 3269, 3394, 3697, 4528, 4872, 5155, 5905, 5956
BseMI GCAATG 7 cut(s) 2031, 2787, 3111, 3330, 3342, 4518, 5081
BseRI GAGGAG 3 cut(s) 792, 843, 5547
BseSI GKGCMC 2 cut(s) 2428, 3810
BseYI CCCAGC 3 cut(s) 5178, 5445, 5563
BsgI GTGCAG 3 cut(s) 2397, 2985, 4863
Bsh1236I CGCG 2 cut(s) 1096, 5127
Bsh1285I CGRYCG 1 cut(s) 4816
BshNI GGYRCC 2 cut(s) 2699, 5066
BshTI ACCGGT 1 cut(s) 2791
BshVI ATCGAT 2 cut(s) 102, 3966
BsiEI CGRYCG 1 cut(s) 4816
BsiHKAI GWGCWC 9 cut(s) 675, 930, 1841, 2190, 2428, 2791, 4138, 4688, 4713
BsiSI CCGG 9 cut(s) 203, 315, 687, 2792, 3216, 3925, 4080, 5053, 5684
BslFI GGGAC 8 cut(s) 703, 1438, 1934, 2724, 3255, 5809, 5922, 6037
BslI CCNNNNNNNGG 9 cut(s) 2459, 3269, 3394, 3697, 4528, 4872, 5155, 5905, 5956
BsmAI GTCTC 7 cut(s) 155, 1571, 2260, 2838, 4345, 5210, 6004
BsmBI CGTCTC 1 cut(s) 155
BsmFI GGGAC 8 cut(s) 703, 1438, 1934, 2724, 3255, 5809, 5922, 6037
BsmI GAATGC 3 cut(s) 2814, 2873, 5518
Bsp119I TTCGAA 1 cut(s) 1600
Bsp13I TCCGGA 2 cut(s) 3215, 5052
Bsp1407I TGTACA 3 cut(s) 1711, 3037, 5611
Bsp1720I GCTNAGC 2 cut(s) 924, 4555
Bsp19I CCATGG 1 cut(s) 399
BspDI ATCGAT 2 cut(s) 102, 3966
BspEI TCCGGA 2 cut(s) 3215, 5052
BspFNI CGCG 2 cut(s) 1096, 5127
BspHI TCATGA 2 cut(s) 3715, 4471
BspMAI CTGCAG 6 cut(s) 1567, 2310, 2470, 3594, 3877, 4033
BspMI ACCTGC 1 cut(s) 2419
BspOI GCTAGC 2 cut(s) 941, 4076
BspQI GCTCTTC 3 cut(s) 1829, 3462, 5053
BspT104I TTCGAA 1 cut(s) 1600
BspT107I GGYRCC 2 cut(s) 2699, 5066
BspTI CTTAAG 3 cut(s) 469, 2270, 2704
BsrBI CCGCTC 2 cut(s) 248, 2077
BsrDI GCAATG 7 cut(s) 2031, 2787, 3111, 3330, 3342, 4518, 5081
BsrFI RCCGGY 4 cut(s) 202, 314, 2791, 5683
BsrGI TGTACA 3 cut(s) 1711, 3037, 5611
BssAI RCCGGY 4 cut(s) 202, 314, 2791, 5683
BssECI CCNNGG 8 cut(s) 47, 399, 1062, 1109, 1455, 1512, 1951, 5759
BssNAI GTATAC 1 cut(s) 4170
BssSI CACGAG 1 cut(s) 4365
BssT1I CCWWGG 3 cut(s) 399, 1062, 5759
Bst1107I GTATAC 1 cut(s) 4170
Bst2BI CACGAG 1 cut(s) 4365
BstAFI CTTAAG 3 cut(s) 469, 2270, 2704
BstAPI GCANNNNNTGC 5 cut(s) 224, 233, 1652, 3113, 3872
BstAUI TGTACA 3 cut(s) 1711, 3037, 5611
BstBAI YACGTR 1 cut(s) 2546
BstBI TTCGAA 1 cut(s) 1600
BstDSI CCRYGG 2 cut(s) 47, 399
BstEII GGTNACC 1 cut(s) 5144
BstENI CCTNNNNNAGG 2 cut(s) 3695, 4526
BstFNI CGCG 2 cut(s) 1096, 5127
BstH2I RGCGCY 1 cut(s) 1160
BstHHI GCGC 5 cut(s) 1159, 1541, 1946, 2667, 2976
BstMAI GTCTC 7 cut(s) 155, 1571, 2260, 2838, 4345, 5210, 6004
BstMCI CGRYCG 1 cut(s) 4816
BstNSI RCATGY 6 cut(s) 1621, 3111, 3733, 3754, 4673, 5833
BstPAI GACNNNNGTC 2 cut(s) 147, 2308
BstPI GGTNACC 1 cut(s) 5144
BstSFI CTRYAG 8 cut(s) 789, 1563, 2306, 2466, 3590, 3873, 4029, 5163
BstSLI GKGCMC 2 cut(s) 2428, 3810
BstUI CGCG 2 cut(s) 1096, 5127
BstV2I GAAGAC 4 cut(s) 185, 332, 1755, 2531
BstX2I RGATCY 8 cut(s) 716, 1670, 2980, 3218, 3493, 3529, 4432, 4861
BstXI CCANNNNNNTGG 3 cut(s) 1085, 2678, 4398
BstYI RGATCY 8 cut(s) 716, 1670, 2980, 3218, 3493, 3529, 4432, 4861
BstZ17I GTATAC 1 cut(s) 4170
Bsu15I ATCGAT 2 cut(s) 102, 3966
Bsu36I CCTNAGG 1 cut(s) 804
BsuI GTATCC 5 cut(s) 637, 763, 1810, 3740, 5024
BsuTUI ATCGAT 2 cut(s) 102, 3966
BtgI CCRYGG 2 cut(s) 47, 399
BtsI GCAGTG 6 cut(s) 1527, 1560, 2019, 2414, 2475, 4513
BveI ACCTGC 1 cut(s) 2419
CaiI CAGNNNCTG 3 cut(s) 1652, 5522, 5849
CciI TCATGA 2 cut(s) 3715, 4471
CfoI GCGC 5 cut(s) 1159, 1541, 1946, 2667, 2976
Cfr10I RCCGGY 4 cut(s) 202, 314, 2791, 5683
ClaI ATCGAT 2 cut(s) 102, 3966
CseI GACGC 1 cut(s) 392
CspAI ACCGGT 1 cut(s) 2791
DraI TTTAAA 3 cut(s) 513, 1323, 1879
DraIII CACNNNGTG 1 cut(s) 1845
DrdI GACNNNNNNGTC 1 cut(s) 5916
DseDI GACNNNNNNGTC 1 cut(s) 5916
EaeI YGGCCR 1 cut(s) 3846
EciI GGCGGA 1 cut(s) 203
Ecl136II GAGCTC 3 cut(s) 673, 928, 4711
Eco130I CCWWGG 3 cut(s) 399, 1062, 5759
Eco147I AGGCCT 4 cut(s) 1441, 1636, 3398, 5733
Eco24I GRGCYC 4 cut(s) 675, 930, 2600, 4713
Eco32I GATATC 1 cut(s) 2089
Eco47I GGWCC 8 cut(s) 767, 1452, 2144, 2375, 3242, 3327, 4611, 4996
Eco53kI GAGCTC 3 cut(s) 673, 928, 4711
Eco57I CTGAAG 8 cut(s) 1566, 1767, 2154, 2498, 2603, 3066, 3505, 4570
Eco72I CACGTG 1 cut(s) 2546
Eco81I CCTNAGG 1 cut(s) 804
Eco91I GGTNACC 1 cut(s) 5144
EcoICRI GAGCTC 3 cut(s) 673, 928, 4711
EcoNI CCTNNNNNAGG 2 cut(s) 3695, 4526
EcoO109I RGGNCCY 3 cut(s) 1066, 1452, 4611
EcoO65I GGTNACC 1 cut(s) 5144
EcoRV GATATC 1 cut(s) 2089
EcoT14I CCWWGG 3 cut(s) 399, 1062, 5759
EcoT22I ATGCAT 3 cut(s) 229, 1185, 3113
EcoT38I GRGCYC 4 cut(s) 675, 930, 2600, 4713
ErhI CCWWGG 3 cut(s) 399, 1062, 5759
Esp3I CGTCTC 1 cut(s) 155
FaqI GGGAC 8 cut(s) 703, 1438, 1934, 2724, 3255, 5809, 5922, 6037
FauI CCCGC 1 cut(s) 2070
FauNDI CATATG 4 cut(s) 2859, 4106, 4746, 5689
FbaI TGATCA 1 cut(s) 4438
FblI GTMKAC 3 cut(s) 2059, 4169, 4487
FriOI GRGCYC 4 cut(s) 675, 930, 2600, 4713
GlaI GCGC 5 cut(s) 1158, 1540, 1945, 2666, 2975
GsaI CCCAGC 3 cut(s) 5182, 5449, 5567
GsuI CTGGAG 5 cut(s) 1478, 1595, 3840, 4869, 5879
HaeII RGCGCY 1 cut(s) 1160
HapII CCGG 9 cut(s) 203, 315, 687, 2792, 3216, 3925, 4080, 5053, 5684
HgaI GACGC 1 cut(s) 392
HhaI GCGC 5 cut(s) 1159, 1541, 1946, 2667, 2976
Hin6I GCGC 5 cut(s) 1157, 1539, 1944, 2665, 2974
HinP1I GCGC 5 cut(s) 1157, 1539, 1944, 2665, 2974
HincII GTYRAC 6 cut(s) 1210, 2997, 3756, 4488, 5241, 5755
HindII GTYRAC 6 cut(s) 1210, 2997, 3756, 4488, 5241, 5755
HpaII CCGG 9 cut(s) 203, 315, 687, 2792, 3216, 3925, 4080, 5053, 5684
Hpy99I CGWCG 1 cut(s) 10
HpyCH4IV ACGT 5 cut(s) 1752, 2545, 4852, 4898, 5477
HpySE526I ACGT 5 cut(s) 1752, 2545, 4852, 4898, 5477
HspAI GCGC 5 cut(s) 1157, 1539, 1944, 2665, 2974
Kpn2I TCCGGA 2 cut(s) 3215, 5052
Ksp22I TGATCA 1 cut(s) 4438
LguI GCTCTTC 3 cut(s) 1829, 3462, 5053
MaeII ACGT 5 cut(s) 1752, 2545, 4852, 4898, 5477
MbiI CCGCTC 2 cut(s) 248, 2077
MfeI CAATTG 3 cut(s) 447, 1829, 2625
MflI RGATCY 8 cut(s) 716, 1670, 2980, 3218, 3493, 3529, 4432, 4861
MlsI TGGCCA 1 cut(s) 3848
MluNI TGGCCA 1 cut(s) 3848
MmeI TCCRAC 7 cut(s) 575, 1937, 2511, 3250, 3629, 3934, 4328
Mox20I TGGCCA 1 cut(s) 3848
Mph1103I ATGCAT 3 cut(s) 229, 1185, 3113
MroI TCCGGA 2 cut(s) 3215, 5052
MroXI GAANNNNTTC 3 cut(s) 3486, 3518, 3711
MscI TGGCCA 1 cut(s) 3848
MslI CAYNNNNRTG 6 cut(s) 895, 2024, 3335, 4637, 4749, 5274
Msp20I TGGCCA 1 cut(s) 3848
MspA1I CMGCKG 5 cut(s) 1190, 1610, 2237, 4357, 5522
MspCI CTTAAG 3 cut(s) 469, 2270, 2704
MspI CCGG 9 cut(s) 203, 315, 687, 2792, 3216, 3925, 4080, 5053, 5684
MunI CAATTG 3 cut(s) 447, 1829, 2625
Mva1269I GAATGC 3 cut(s) 2814, 2873, 5518
MvnI CGCG 2 cut(s) 1096, 5127
NciI CCSGG 1 cut(s) 688
NcoI CCATGG 1 cut(s) 399
NdeI CATATG 4 cut(s) 2859, 4106, 4746, 5689
NheI GCTAGC 2 cut(s) 937, 4072
NmeAIII GCCGAG 1 cut(s) 1029
NmuCI GTSAC 7 cut(s) 965, 1282, 1558, 3390, 5144, 5366, 5785
NsiI ATGCAT 3 cut(s) 229, 1185, 3113
NspI RCATGY 6 cut(s) 1621, 3111, 3733, 3754, 4673, 5833
NspV TTCGAA 1 cut(s) 1600
PaeI GCATGC 3 cut(s) 1621, 3111, 5833
PagI TCATGA 2 cut(s) 3715, 4471
PceI AGGCCT 4 cut(s) 1441, 1636, 3398, 5733
PciI ACATGT 2 cut(s) 3729, 3750
PciSI GCTCTTC 3 cut(s) 1829, 3462, 5053
PctI GAATGC 3 cut(s) 2814, 2873, 5518
PdmI GAANNNNTTC 3 cut(s) 3486, 3518, 3711
PflFI GACNNNGTC 1 cut(s) 3760
PflMI CCANNNNNTGG 1 cut(s) 3269
PfoI TCCNGGA 1 cut(s) 2146
PinAI ACCGGT 1 cut(s) 2791
PmaCI CACGTG 1 cut(s) 2546
PmlI CACGTG 1 cut(s) 2546
Ppu21I YACGTR 1 cut(s) 2546
PpuMI RGGWCCY 2 cut(s) 1452, 4611
PscI ACATGT 2 cut(s) 3729, 3750
PshAI GACNNNNGTC 2 cut(s) 147, 2308
PshBI ATTAAT 2 cut(s) 1928, 5879
PsiI TTATAA 4 cut(s) 1004, 1808, 3027, 4086
Psp124BI GAGCTC 3 cut(s) 675, 930, 4713
Psp1406I AACGTT 1 cut(s) 4898
Psp5II RGGWCCY 2 cut(s) 1452, 4611
PspCI CACGTG 1 cut(s) 2546
PspEI GGTNACC 1 cut(s) 5144
PspFI CCCAGC 3 cut(s) 5178, 5445, 5563
PspPPI RGGWCCY 2 cut(s) 1452, 4611
PstI CTGCAG 6 cut(s) 1567, 2310, 2470, 3594, 3877, 4033
PstNI CAGNNNCTG 3 cut(s) 1652, 5522, 5849
PsuI RGATCY 8 cut(s) 716, 1670, 2980, 3218, 3493, 3529, 4432, 4861
PsyI GACNNNGTC 1 cut(s) 3760
PvuII CAGCTG 5 cut(s) 1190, 1610, 2237, 4357, 5522
RseI CAYNNNNRTG 6 cut(s) 895, 2024, 3335, 4637, 4749, 5274
SacI GAGCTC 3 cut(s) 675, 930, 4713
SalI GTCGAC 1 cut(s) 4486
SapI GCTCTTC 3 cut(s) 1829, 3462, 5053
ScaI AGTACT 1 cut(s) 3518
SfcI CTRYAG 8 cut(s) 789, 1563, 2306, 2466, 3590, 3873, 4029, 5163
SfuI TTCGAA 1 cut(s) 1600
SinI GGWCC 8 cut(s) 767, 1452, 2144, 2375, 3242, 3327, 4611, 4996
SmiMI CAYNNNNRTG 6 cut(s) 895, 2024, 3335, 4637, 4749, 5274
SmlI CTYRAG 9 cut(s) 469, 581, 1118, 2270, 2704, 3497, 3581, 4867, 5213
SmoI CTYRAG 9 cut(s) 469, 581, 1118, 2270, 2704, 3497, 3581, 4867, 5213
SpeI ACTAGT 1 cut(s) 5789
SphI GCATGC 3 cut(s) 1621, 3111, 5833
SseBI AGGCCT 4 cut(s) 1441, 1636, 3398, 5733
SspI AATATT 1 cut(s) 2220
SstI GAGCTC 3 cut(s) 675, 930, 4713
StuI AGGCCT 4 cut(s) 1441, 1636, 3398, 5733
StyI CCWWGG 3 cut(s) 399, 1062, 5759
TaiI ACGT 5 cut(s) 1755, 2548, 4855, 4901, 5480
TaqI TCGA 8 cut(s) 102, 366, 1268, 1600, 2491, 3966, 4487, 4989
TaqII GACCGA 1 cut(s) 3773
TatI WGTACW 5 cut(s) 1711, 3037, 3516, 3551, 5611
TauI GCSGC 4 cut(s) 251, 3544, 5228, 5843
TseFI GTSAC 7 cut(s) 965, 1282, 1558, 3390, 5144, 5366, 5785
Tsp45I GTSAC 7 cut(s) 965, 1282, 1558, 3390, 5144, 5366, 5785
TspGWI ACGGA 3 cut(s) 143, 2414, 3086
Tth111I GACNNNGTC 1 cut(s) 3760
Van91I CCANNNNNTGG 1 cut(s) 3269
Vha464I CTTAAG 3 cut(s) 469, 2270, 2704
VneI GTGCAC 1 cut(s) 2424
VpaK11BI GGWCC 8 cut(s) 767, 1452, 2144, 2375, 3242, 3327, 4611, 4996
VspI ATTAAT 2 cut(s) 1928, 5879
XagI CCTNNNNNAGG 2 cut(s) 3695, 4526
XceI RCATGY 6 cut(s) 1621, 3111, 3733, 3754, 4673, 5833
XcmI CCANNNNNNNNNTGG 2 cut(s) 103, 4751
XmiI GTMKAC 3 cut(s) 2059, 4169, 4487
XmnI GAANNNNTTC 3 cut(s) 3486, 3518, 3711
ZrmI AGTACT 1 cut(s) 3518
Zsp2I ATGCAT 3 cut(s) 229, 1185, 3113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.