Rh5AG270400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002916
Physical Location & Seq
Forward (+)
35856592 .. 35857125
534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG270400.1

Sequence Viewer

Length: 534 bp
ATGTTAGAGCAGTATATAAAGAAAGGGAACCCTAGCCTTTCCTCCTTTTCTCCTTCTTCCTCTGTCGCTCCACACACGCCTCTCTCCCTTTTCCTCTCACCTTCTCTCTGTCACACCGGAAAGCTCGACTTCCAGATGTCTACTTTTGACACTGCCACCATTTCCTCTCACCTTCTCTCTGTCACACTGGAAAGCTCGACTTCCGGATGTCTACTTTTGACACTGCCACCACCAAAAGATAGCCTAGGCGACGGAGAACAAAACCCAGCCACCTCACCCTCGATCGAGATGACCAGACCATCGCACCCAACCCCTAAAACTACGGCGTCGCTGTTCCTTAACCTCTTCGATCTTCTCTCTAGGTTCGCAGCTCTTCCATCCTGTCACCGTGCCCTTCGACCTCAGCCTATGCAGCAATGCCTTCGACTTGCCGGACACCGATCCCAGGGCCGCATTAAGGGATTTGAGCCCGAGCAGATTTCGGCACTCTCTCCGCCAGTTACGATTTCTAAGGATCTTTTGGATTACAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

19.2

Weight (kDa)

8.42

Isoelectric Point (pI)

64.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 140, 211
AccIII TCCGGA 1 cut(s) 203
AciI CCGC 2 cut(s) 451, 494
AclWI GGATC 2 cut(s) 435, 522
AcyI GRCGYC 1 cut(s) 326
AfiI CCNNNNNNNGG 2 cut(s) 445, 457
AjnI CCWGG 1 cut(s) 444
AluBI AGCT 3 cut(s) 124, 195, 371
AluI AGCT 3 cut(s) 124, 195, 371
AlwI GGATC 2 cut(s) 435, 522
Ama87I CYCGRG 1 cut(s) 470
Aor13HI TCCGGA 1 cut(s) 203
AoxI GGCC 1 cut(s) 448
ApeKI GCWGC 2 cut(s) 368, 412
AspA2I CCTAGG 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 448
AsuHPI GGTGA 4 cut(s) 90, 161, 267, 377
AvaI CYCGRG 1 cut(s) 470
AvrII CCTAGG 1 cut(s) 244
BaeGI GKGCMC 1 cut(s) 394
BanII GRGCYC 1 cut(s) 471
BbvCI CCTCAGC 1 cut(s) 402
BbvI GCAGC 2 cut(s) 380, 424
BccI CCATC 2 cut(s) 307, 385
BceAI ACGGC 1 cut(s) 339
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BciT130I CCWGG 1 cut(s) 446
BfaI CTAG 3 cut(s) 33, 245, 360
BisI GCNGC 3 cut(s) 369, 413, 451
BlnI CCTAGG 1 cut(s) 244
BlsI GCNGC 3 cut(s) 370, 414, 452
Bme1390I CCNGG 1 cut(s) 446
BmeT110I CYCGRG 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 448
BmiI GGNNCC 1 cut(s) 29
BmrFI CCNGG 1 cut(s) 446
Bpu10I CCTNAGC 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 326
BsaJI CCNNGG 3 cut(s) 244, 444, 445
BsaWI WCCGGW 2 cut(s) 116, 203
Bsc4I CCNNNNNNNGG 2 cut(s) 445, 457
Bse1I ACTGG 2 cut(s) 192, 497
Bse3DI GCAATG 1 cut(s) 422
BseAI TCCGGA 1 cut(s) 203
BseBI CCWGG 1 cut(s) 446
BseDI CCNNGG 3 cut(s) 244, 444, 445
BseGI GGATG 2 cut(s) 212, 377
BseLI CCNNNNNNNGG 2 cut(s) 445, 457
BseMI GCAATG 1 cut(s) 422
BseMII CTCAG 1 cut(s) 416
BseNI ACTGG 2 cut(s) 192, 497
BseSI GKGCMC 1 cut(s) 394
BseXI GCAGC 2 cut(s) 380, 424
BseYI CCCAGC 1 cut(s) 265
Bsh1285I CGRYCG 1 cut(s) 285
BshFI GGCC 1 cut(s) 450
BsiEI CGRYCG 1 cut(s) 285
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 3 cut(s) 117, 204, 432
BslI CCNNNNNNNGG 2 cut(s) 445, 457
BsnI GGCC 1 cut(s) 450
BsoBI CYCGRG 1 cut(s) 470
Bsp1286I GDGCHC 2 cut(s) 394, 471
Bsp13I TCCGGA 1 cut(s) 203
Bsp143I GATC 4 cut(s) 282, 349, 440, 514
BspACI CCGC 2 cut(s) 451, 494
BspANI GGCC 1 cut(s) 450
BspCNI CTCAG 1 cut(s) 415
BspEI TCCGGA 1 cut(s) 203
BspLI GGNNCC 1 cut(s) 29
BspPI GGATC 2 cut(s) 435, 522
BspQI GCTCTTC 1 cut(s) 378
BsrDI GCAATG 1 cut(s) 422
BsrI ACTGG 2 cut(s) 192, 497
BssECI CCNNGG 3 cut(s) 244, 444, 445
BssMI GATC 4 cut(s) 282, 349, 440, 514
BssNI GRCGYC 1 cut(s) 326
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 1 cut(s) 389
Bst6I CTCTTC 2 cut(s) 350, 378
BstACI GRCGYC 1 cut(s) 326
BstDEI CTNAG 2 cut(s) 402, 510
BstF5I GGATG 2 cut(s) 212, 377
BstKTI GATC 4 cut(s) 285, 352, 443, 517
BstMBI GATC 4 cut(s) 282, 349, 440, 514
BstMCI CGRYCG 1 cut(s) 285
BstMWI GCNNNNNNNGC 1 cut(s) 412
BstNI CCWGG 1 cut(s) 446
BstSCI CCNGG 1 cut(s) 444
BstSLI GKGCMC 1 cut(s) 394
BstV1I GCAGC 2 cut(s) 380, 424
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
BsuRI GGCC 1 cut(s) 450
BtgZI GCGATG 1 cut(s) 285
BtsCI GGATG 2 cut(s) 212, 377
BtsI GCAGTG 2 cut(s) 150, 221
BtsIMutI CAGTG 3 cut(s) 150, 185, 221
Cfr13I GGNCC 1 cut(s) 448
CseI GACGC 1 cut(s) 315
CviJI RGCY 9 cut(s) 36, 124, 195, 243, 269, 371, 406, 450, 469
CviKI_1 RGCY 9 cut(s) 36, 124, 195, 243, 269, 371, 406, 450, 469
DdeI CTNAG 2 cut(s) 402, 510
DpnI GATC 4 cut(s) 284, 351, 442, 516
DpnII GATC 4 cut(s) 282, 349, 440, 514
Eam1104I CTCTTC 2 cut(s) 350, 378
EarI CTCTTC 2 cut(s) 350, 378
EciI GGCGGA 1 cut(s) 483
Eco130I CCWWGG 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 444
EcoT14I CCWWGG 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 471
ErhI CCWWGG 1 cut(s) 244
FaiI YATR 3 cut(s) 15, 17, 410
FalI AAGNNNNNCTT 4 cut(s) 113, 145, 184, 216
FblI GTMKAC 2 cut(s) 140, 211
Fnu4HI GCNGC 3 cut(s) 369, 413, 451
FokI GGATG 2 cut(s) 219, 364
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 3 cut(s) 369, 413, 451
FspBI CTAG 3 cut(s) 33, 245, 360
GluI GCNGC 3 cut(s) 369, 413, 451
GsaI CCCAGC 1 cut(s) 269
HaeIII GGCC 1 cut(s) 450
HapII CCGG 3 cut(s) 117, 204, 432
HgaI GACGC 1 cut(s) 315
Hin1I GRCGYC 1 cut(s) 326
HpaII CCGG 3 cut(s) 117, 204, 432
HphI GGTGA 4 cut(s) 90, 161, 267, 377
Hpy166II GTNNAC 2 cut(s) 141, 212
Hpy188III TCNNGA 3 cut(s) 133, 204, 286
Hpy8I GTNNAC 2 cut(s) 141, 212
Hpy99I CGWCG 2 cut(s) 254, 331
HpyAV CCTTC 5 cut(s) 63, 111, 182, 404, 431
HpyCH4III ACNGT 1 cut(s) 389
HpyCH4V TGCA 1 cut(s) 412
HpyF10VI GCNNNNNNNGC 1 cut(s) 412
HpyF3I CTNAG 2 cut(s) 402, 510
Hsp92I GRCGYC 1 cut(s) 326
Kpn2I TCCGGA 1 cut(s) 203
Kzo9I GATC 4 cut(s) 282, 349, 440, 514
LguI GCTCTTC 1 cut(s) 378
LmnI GCTCC 1 cut(s) 73
Lsp1109I GCAGC 2 cut(s) 380, 424
MaeI CTAG 3 cut(s) 33, 245, 360
MaeIII GTNAC 4 cut(s) 110, 181, 383, 499
MalI GATC 4 cut(s) 284, 351, 442, 516
MboI GATC 4 cut(s) 282, 349, 440, 514
MboII GAAGA 4 cut(s) 48, 337, 344, 365
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 2 cut(s) 394, 471
MnlI CCTC 9 cut(s) 52, 70, 90, 104, 175, 283, 289, 353, 411
MroI TCCGGA 1 cut(s) 203
MseI TTAA 2 cut(s) 339, 456
MspI CCGG 3 cut(s) 117, 204, 432
MspR9I CCNGG 1 cut(s) 446
MvaI CCWGG 1 cut(s) 446
MwoI GCNNNNNNNGC 1 cut(s) 412
NdeII GATC 4 cut(s) 282, 349, 440, 514
NlaIV GGNNCC 1 cut(s) 29
NmuCI GTSAC 3 cut(s) 110, 181, 383
PasI CCCWGGG 1 cut(s) 445
PciSI GCTCTTC 1 cut(s) 378
PkrI GCNGC 3 cut(s) 370, 414, 452
Ple19I CGATCG 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 444
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 1 cut(s) 29
PspPI GGNCC 1 cut(s) 448
PsuI RGATCY 1 cut(s) 514
PvuI CGATCG 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 378
SaqAI TTAA 2 cut(s) 339, 456
SatI GCNGC 3 cut(s) 369, 413, 451
Sau3AI GATC 4 cut(s) 282, 349, 440, 514
Sau96I GGNCC 1 cut(s) 448
ScrFI CCNGG 1 cut(s) 446
SduI GDGCHC 2 cut(s) 394, 471
SsiI CCGC 2 cut(s) 451, 494
SspMI CTAG 3 cut(s) 33, 245, 360
StyD4I CCNGG 1 cut(s) 444
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 1 cut(s) 389
TaqI TCGA 7 cut(s) 126, 197, 281, 285, 348, 397, 424
TauI GCSGC 1 cut(s) 453
Tru1I TTAA 2 cut(s) 339, 456
Tru9I TTAA 2 cut(s) 339, 456
TscAI CASTG 3 cut(s) 157, 192, 228
TseFI GTSAC 3 cut(s) 110, 181, 383
TseI GCWGC 2 cut(s) 368, 412
Tsp45I GTSAC 3 cut(s) 110, 181, 383
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 3 cut(s) 157, 192, 228
XmaJI CCTAGG 1 cut(s) 244
XmiI GTMKAC 2 cut(s) 140, 211
XspI CTAG 3 cut(s) 33, 245, 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.