Rorug02G0281900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002771
Physical Location & Seq
Forward (+)
30399863 .. 30400845
983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0281900.1

Sequence Viewer

Length: 486 bp
ATGGCAGCCATGAACTCTAGCGTTTTGGCAAGTAACTATGCCATCTCTGGCAGCAGTGCAGTACTGGCTGAAGCCAATGGCAAGATGACCTCCATGCCTCAGAGAGTAGCTATGCCTGTAATTAGGGCCCAACATCAAGAGTCGCAAAGCGTTGGAGGAAGAAGAGCTGCACTGCTCTTCCTCACCGCCACTGCGGCTGCTGTTGCCTCCAACTCCATTGCCAATGCCAGCGTCTTTGACGACTACCTGGAGAAGAGCAAGGCCAACAAGGAACTCAATGACAAGAAGAGGCTTGCAACAAGTGGAGCAAACTTTGCAAGAGCATTCACTGTTCAATTTGGGACCTGCAAATTCCCTGAGAACTTCACTGGCTGCCAAGATCTTGCCAAGCAAAATAAGGTGCCATTTCTCTCTGACGATCTGAAGTTGGAATGCGAAGGCAAGGACAAGTACAAGTGTGGTTCCAATGTATTCTGGAAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

17.4

Weight (kDa)

9.23

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaN PF05479 41 - 161 1e-61 Photosystem I reaction centre subunit N (PSAN or PSI-N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 353
AccB1I GGYRCC 1 cut(s) 400
AciI CCGC 2 cut(s) 186, 194
AcsI RAATTY 1 cut(s) 350
AcuI CTGAAG 2 cut(s) 90, 443
AfaI GTAC 2 cut(s) 63, 452
AgsI TTSAA 1 cut(s) 335
AjnI CCWGG 1 cut(s) 246
AluBI AGCT 2 cut(s) 110, 167
AluI AGCT 2 cut(s) 110, 167
AoxI GGCC 2 cut(s) 126, 261
ApaI GGGCCC 1 cut(s) 130
ApeKI GCWGC 5 cut(s) 5, 51, 167, 197, 372
ApoI RAATTY 1 cut(s) 350
AspS9I GGNCC 3 cut(s) 126, 127, 342
AsuHPI GGTGA 1 cut(s) 175
AvaII GGWCC 1 cut(s) 342
BaeGI GKGCMC 1 cut(s) 130
BanI GGYRCC 1 cut(s) 400
BanII GRGCYC 1 cut(s) 130
BbvI GCAGC 5 cut(s) 17, 63, 154, 184, 359
BccI CCATC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 248
BfaI CTAG 1 cut(s) 18
BfuAI ACCTGC 1 cut(s) 353
BglI GCCNNNNNGGC 1 cut(s) 194
BglII AGATCT 1 cut(s) 379
BisI GCNGC 6 cut(s) 6, 52, 168, 195, 198, 373
BlsI GCNGC 6 cut(s) 7, 53, 169, 196, 199, 374
BmcAI AGTACT 1 cut(s) 63
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 1 cut(s) 342
BmgT120I GGNCC 3 cut(s) 126, 127, 342
BmiI GGNNCC 4 cut(s) 128, 343, 402, 463
BmrFI CCNGG 1 cut(s) 248
BpmI CTGGAG 1 cut(s) 269
Bse1I ACTGG 2 cut(s) 69, 373
Bse3DI GCAATG 1 cut(s) 216
BseBI CCWGG 1 cut(s) 248
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 2 cut(s) 113, 348
BseNI ACTGG 2 cut(s) 69, 373
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 5 cut(s) 17, 63, 154, 184, 359
BsgI GTGCAG 2 cut(s) 78, 153
BshFI GGCC 2 cut(s) 128, 263
BshNI GGYRCC 1 cut(s) 400
BslFI GGGAC 1 cut(s) 355
BsmFI GGGAC 1 cut(s) 355
BsmI GAATGC 2 cut(s) 323, 437
BsnI GGCC 2 cut(s) 128, 263
Bsp120I GGGCCC 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 130
Bsp143I GATC 2 cut(s) 379, 418
BspACI CCGC 2 cut(s) 186, 194
BspANI GGCC 2 cut(s) 128, 263
BspCNI CTCAG 2 cut(s) 112, 349
BspLI GGNNCC 4 cut(s) 128, 343, 402, 463
BspMI ACCTGC 1 cut(s) 353
BspQI GCTCTTC 3 cut(s) 157, 182, 248
BspT107I GGYRCC 1 cut(s) 400
BsrDI GCAATG 1 cut(s) 216
BsrI ACTGG 2 cut(s) 69, 373
BssMI GATC 2 cut(s) 379, 418
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 1 cut(s) 331
Bst6I CTCTTC 4 cut(s) 157, 182, 248, 281
BstAPI GCANNNNNTGC 1 cut(s) 314
BstC8I GCNNGC 2 cut(s) 229, 294
BstDEI CTNAG 2 cut(s) 99, 357
BstKTI GATC 2 cut(s) 382, 421
BstMBI GATC 2 cut(s) 379, 418
BstMWI GCNNNNNNNGC 4 cut(s) 65, 194, 203, 314
BstNI CCWGG 1 cut(s) 248
BstSCI CCNGG 1 cut(s) 246
BstSLI GKGCMC 1 cut(s) 130
BstV1I GCAGC 5 cut(s) 17, 63, 154, 184, 359
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BsuRI GGCC 2 cut(s) 128, 263
BtsI GCAGTG 3 cut(s) 61, 170, 189
BtsIMutI CAGTG 5 cut(s) 61, 170, 189, 327, 366
BveI ACCTGC 1 cut(s) 353
Cac8I GCNNGC 2 cut(s) 229, 294
Cfr13I GGNCC 3 cut(s) 126, 127, 342
CseI GACGC 1 cut(s) 220
Csp6I GTAC 2 cut(s) 62, 451
CviAII CATG 2 cut(s) 10, 94
CviQI GTAC 2 cut(s) 62, 451
DdeI CTNAG 2 cut(s) 99, 357
DpnI GATC 2 cut(s) 381, 420
DpnII GATC 2 cut(s) 379, 418
Eam1104I CTCTTC 4 cut(s) 157, 182, 248, 281
EarI CTCTTC 4 cut(s) 157, 182, 248, 281
Eco24I GRGCYC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 342
Eco57I CTGAAG 2 cut(s) 90, 443
EcoO109I RGGNCCY 2 cut(s) 126, 342
EcoRII CCWGG 1 cut(s) 246
EcoT38I GRGCYC 1 cut(s) 130
FaeI CATG 2 cut(s) 13, 97
FaiI YATR 4 cut(s) 11, 39, 95, 113
FaqI GGGAC 1 cut(s) 355
FatI CATG 2 cut(s) 9, 93
Fnu4HI GCNGC 6 cut(s) 6, 52, 168, 195, 198, 373
FriOI GRGCYC 1 cut(s) 130
Fsp4HI GCNGC 6 cut(s) 6, 52, 168, 195, 198, 373
FspBI CTAG 1 cut(s) 18
GluI GCNGC 6 cut(s) 6, 52, 168, 195, 198, 373
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 2 cut(s) 128, 263
HgaI GACGC 1 cut(s) 220
Hin1II CATG 2 cut(s) 13, 97
HinfI GANTC 1 cut(s) 140
HphI GGTGA 1 cut(s) 175
Hpy188I TCNGA 3 cut(s) 102, 415, 423
Hpy188III TCNNGA 2 cut(s) 137, 475
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4V TGCA 5 cut(s) 59, 170, 296, 317, 348
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 194, 203, 314
HpyF3I CTNAG 2 cut(s) 99, 357
Hsp92II CATG 2 cut(s) 13, 97
Kzo9I GATC 2 cut(s) 379, 418
LguI GCTCTTC 3 cut(s) 157, 182, 248
LmnI GCTCC 1 cut(s) 305
Lsp1109I GCAGC 5 cut(s) 17, 63, 154, 184, 359
MaeI CTAG 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 32
MalI GATC 2 cut(s) 381, 420
MboI GATC 2 cut(s) 379, 418
MboII GAAGA 5 cut(s) 169, 171, 174, 265, 298
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 3 cut(s) 120, 335, 350
MlyI GAGTC 1 cut(s) 149
MmeI TCCRAC 3 cut(s) 133, 234, 408
MnlI CCTC 6 cut(s) 100, 108, 149, 191, 217, 282
MspR9I CCNGG 1 cut(s) 248
Mva1269I GAATGC 2 cut(s) 323, 437
MvaI CCWGG 1 cut(s) 248
MwoI GCNNNNNNNGC 4 cut(s) 65, 194, 203, 314
NdeII GATC 2 cut(s) 379, 418
NlaIII CATG 2 cut(s) 13, 97
NlaIV GGNNCC 4 cut(s) 128, 343, 402, 463
PciSI GCTCTTC 3 cut(s) 157, 182, 248
PctI GAATGC 2 cut(s) 323, 437
PkrI GCNGC 6 cut(s) 7, 53, 169, 196, 199, 374
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PpuMI RGGWCCY 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 246
PspGI CCWGG 1 cut(s) 246
PspN4I GGNNCC 4 cut(s) 128, 343, 402, 463
PspOMI GGGCCC 1 cut(s) 126
PspPI GGNCC 3 cut(s) 126, 127, 342
PspPPI RGGWCCY 1 cut(s) 342
PsuI RGATCY 1 cut(s) 379
RsaI GTAC 2 cut(s) 63, 452
RsaNI GTAC 2 cut(s) 62, 451
SapI GCTCTTC 3 cut(s) 157, 182, 248
SatI GCNGC 6 cut(s) 6, 52, 168, 195, 198, 373
Sau3AI GATC 2 cut(s) 379, 418
Sau96I GGNCC 3 cut(s) 126, 127, 342
ScaI AGTACT 1 cut(s) 63
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 1 cut(s) 130
SetI ASST 6 cut(s) 92, 112, 169, 249, 347, 402
SinI GGWCC 1 cut(s) 342
Sse9I AATT 3 cut(s) 120, 335, 350
SsiI CCGC 2 cut(s) 186, 194
SspMI CTAG 1 cut(s) 18
StyD4I CCNGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 331
TasI AATT 3 cut(s) 120, 335, 350
TatI WGTACW 2 cut(s) 61, 450
TauI GCSGC 1 cut(s) 197
TscAI CASTG 5 cut(s) 61, 177, 196, 334, 373
TseI GCWGC 5 cut(s) 5, 51, 167, 197, 372
TspDTI ATGAA 1 cut(s) 26
TspRI CASTG 5 cut(s) 61, 177, 196, 334, 373
VpaK11BI GGWCC 1 cut(s) 342
XapI RAATTY 1 cut(s) 350
XspI CTAG 1 cut(s) 18
ZrmI AGTACT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.