Rorug02G0410300

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010149
Physical Location & Seq
Reverse (-)
52636205 .. 52636630
426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0410300.1

Sequence Viewer

Length: 375 bp
ATGGCAGAAGGTACGCACGGGTTTCCTGCGTCAGTCATCGATTTGGATTCGTCATTGGTTACGTTGGGGGATGCAGGGGCTGCTGCGTTCCATGATGGCTTTTGGTACATAGTTGGTCGACTGTTGGCCCCAAAAGCTAAGTTTCCAGGATTCAAAGGCACCATTGCTGCTAATTGGCGCCTCAGATCGGGGCTCACTATACAAGATGCAGGTGAGCGCTTTGTTTTCCAGTTTGAAAGTGAAGCAGTGCATAATCGTATACTTCATGGGGGTCCTTGGTTTTATCGCAACACCATGCTGGTGGTGGGAGAGTATGATGGATTCTGTTCGGTGAAGGAAGTGCCGCTGAATACATGGAAACATGGGTTGCAGTGA

Protein Analysis

124

Amino Acids

13.69

Weight (kDa)

6.5

Isoelectric Point (pI)

27.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 35 - 106 7.6e-10 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 200
Acc36I ACCTGC 1 cut(s) 200
AccB1I GGYRCC 2 cut(s) 158, 177
AccI GTMKAC 2 cut(s) 118, 259
AciI CCGC 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 178
AfaI GTAC 2 cut(s) 13, 107
AfeI AGCGCT 1 cut(s) 218
AfiI CCNNNNNNNGG 1 cut(s) 187
AgsI TTSAA 2 cut(s) 154, 236
AjnI CCWGG 1 cut(s) 145
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
AlwNI CAGNNNCTG 1 cut(s) 80
Aor51HI AGCGCT 1 cut(s) 218
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 3 cut(s) 80, 83, 167
AspLEI GCGC 2 cut(s) 180, 219
AspS9I GGNCC 2 cut(s) 127, 272
AsuHPI GGTGA 2 cut(s) 224, 343
AvaII GGWCC 1 cut(s) 272
BanI GGYRCC 2 cut(s) 158, 177
BanII GRGCYC 1 cut(s) 195
BbvI GCAGC 3 cut(s) 67, 70, 154
BccI CCATC 2 cut(s) 89, 311
BciT130I CCWGG 1 cut(s) 147
BfoI RGCGCY 2 cut(s) 181, 220
BfuAI ACCTGC 1 cut(s) 200
BisI GCNGC 4 cut(s) 81, 84, 168, 344
BlsI GCNGC 4 cut(s) 82, 85, 169, 345
Bme1390I CCNGG 1 cut(s) 147
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 2 cut(s) 127, 272
BmiI GGNNCC 4 cut(s) 129, 160, 179, 273
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 2 cut(s) 61, 196
Bsa29I ATCGAT 1 cut(s) 39
BsaHI GRCGYC 1 cut(s) 178
BsaJI CCNNGG 1 cut(s) 275
Bsc4I CCNNNNNNNGG 1 cut(s) 187
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 147
BseCI ATCGAT 1 cut(s) 39
BseDI CCNNGG 1 cut(s) 275
BseGI GGATG 1 cut(s) 76
BseLI CCNNNNNNNGG 1 cut(s) 187
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 196
BseNI ACTGG 1 cut(s) 229
BseXI GCAGC 3 cut(s) 67, 70, 154
BshFI GGCC 1 cut(s) 128
BshNI GGYRCC 2 cut(s) 158, 177
BshVI ATCGAT 1 cut(s) 39
BslI CCNNNNNNNGG 1 cut(s) 187
BsnI GGCC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 195
Bsp143I GATC 1 cut(s) 185
BspACI CCGC 1 cut(s) 344
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 1 cut(s) 195
BspDI ATCGAT 1 cut(s) 39
BspLI GGNNCC 4 cut(s) 129, 160, 179, 273
BspMI ACCTGC 1 cut(s) 200
BspT107I GGYRCC 2 cut(s) 158, 177
BsrDI GCAATG 1 cut(s) 162
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 1 cut(s) 275
BssMI GATC 1 cut(s) 185
BssNAI GTATAC 1 cut(s) 260
BssNI GRCGYC 1 cut(s) 178
BssT1I CCWWGG 1 cut(s) 275
Bst1107I GTATAC 1 cut(s) 260
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 123
BstACI GRCGYC 1 cut(s) 178
BstAPI GCANNNNNTGC 1 cut(s) 80
BstDEI CTNAG 2 cut(s) 138, 182
BstF5I GGATG 1 cut(s) 76
BstH2I RGCGCY 2 cut(s) 181, 220
BstHHI GCGC 2 cut(s) 180, 219
BstKTI GATC 1 cut(s) 188
BstMBI GATC 1 cut(s) 185
BstMWI GCNNNNNNNGC 2 cut(s) 80, 134
BstNI CCWGG 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 145
BstV1I GCAGC 3 cut(s) 67, 70, 154
BstXI CCANNNNNNTGG 1 cut(s) 301
BstZ17I GTATAC 1 cut(s) 260
Bsu15I ATCGAT 1 cut(s) 39
BsuRI GGCC 1 cut(s) 128
BsuTUI ATCGAT 1 cut(s) 39
BtsCI GGATG 1 cut(s) 76
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 1 cut(s) 252
BveI ACCTGC 1 cut(s) 200
CaiI CAGNNNCTG 1 cut(s) 80
CfoI GCGC 2 cut(s) 180, 219
Cfr13I GGNCC 2 cut(s) 127, 272
ClaI ATCGAT 1 cut(s) 39
CseI GACGC 1 cut(s) 18
Csp6I GTAC 2 cut(s) 12, 106
CviAII CATG 5 cut(s) 92, 266, 295, 354, 362
CviJI RGCY 5 cut(s) 80, 99, 128, 137, 193
CviKI_1 RGCY 5 cut(s) 80, 99, 128, 137, 193
CviQI GTAC 2 cut(s) 12, 106
DdeI CTNAG 2 cut(s) 138, 182
DinI GGCGCC 1 cut(s) 179
DpnI GATC 1 cut(s) 187
DpnII GATC 1 cut(s) 185
Eco130I CCWWGG 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 272
Eco47III AGCGCT 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 145
EcoT14I CCWWGG 1 cut(s) 275
EcoT38I GRGCYC 1 cut(s) 195
EgeI GGCGCC 1 cut(s) 179
EheI GGCGCC 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 275
FaeI CATG 5 cut(s) 95, 269, 298, 357, 365
FatI CATG 5 cut(s) 91, 265, 294, 353, 361
FblI GTMKAC 2 cut(s) 118, 259
Fnu4HI GCNGC 4 cut(s) 81, 84, 168, 344
FokI GGATG 1 cut(s) 83
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 4 cut(s) 81, 84, 168, 344
GlaI GCGC 2 cut(s) 179, 218
GluI GCNGC 4 cut(s) 81, 84, 168, 344
HaeII RGCGCY 2 cut(s) 181, 220
HaeIII GGCC 1 cut(s) 128
HgaI GACGC 1 cut(s) 18
HhaI GCGC 2 cut(s) 180, 219
Hin1I GRCGYC 1 cut(s) 178
Hin1II CATG 5 cut(s) 95, 269, 298, 357, 365
Hin6I GCGC 2 cut(s) 178, 217
HinP1I GCGC 2 cut(s) 178, 217
HincII GTYRAC 1 cut(s) 119
HindII GTYRAC 1 cut(s) 119
HinfI GANTC 3 cut(s) 47, 150, 321
HphI GGTGA 2 cut(s) 224, 343
Hpy166II GTNNAC 2 cut(s) 119, 260
Hpy188I TCNGA 1 cut(s) 185
Hpy8I GTNNAC 2 cut(s) 119, 260
HpyAV CCTTC 1 cut(s) 328
HpyCH4III ACNGT 1 cut(s) 123
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 4 cut(s) 74, 209, 250, 370
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 134
HpyF3I CTNAG 2 cut(s) 138, 182
HpySE526I ACGT 1 cut(s) 62
Hsp92I GRCGYC 1 cut(s) 178
Hsp92II CATG 5 cut(s) 95, 269, 298, 357, 365
HspAI GCGC 2 cut(s) 178, 217
KasI GGCGCC 1 cut(s) 177
Kzo9I GATC 1 cut(s) 185
LpnPI CCDG 7 cut(s) 39, 60, 132, 159, 195, 242, 284
Lsp1109I GCAGC 3 cut(s) 67, 70, 154
LweI GCATC 2 cut(s) 61, 196
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 1 cut(s) 58
MalI GATC 1 cut(s) 187
MboI GATC 1 cut(s) 185
MhlI GDGCHC 1 cut(s) 195
MluCI AATT 1 cut(s) 172
Mly113I GGCGCC 1 cut(s) 178
MnlI CCTC 1 cut(s) 191
MslI CAYNNNNRTG 1 cut(s) 299
MspA1I CMGCKG 1 cut(s) 346
MspR9I CCNGG 1 cut(s) 147
MvaI CCWGG 1 cut(s) 147
MwoI GCNNNNNNNGC 2 cut(s) 80, 134
NarI GGCGCC 1 cut(s) 178
NdeII GATC 1 cut(s) 185
NlaIII CATG 5 cut(s) 95, 269, 298, 357, 365
NlaIV GGNNCC 4 cut(s) 129, 160, 179, 273
PaqCI CACCTGC 1 cut(s) 200
PfeI GAWTC 3 cut(s) 47, 150, 321
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 4 cut(s) 82, 85, 169, 345
PluTI GGCGCC 1 cut(s) 181
PpuMI RGGWCCY 1 cut(s) 272
Psp5II RGGWCCY 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 145
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 4 cut(s) 129, 160, 179, 273
PspPI GGNCC 2 cut(s) 127, 272
PspPPI RGGWCCY 1 cut(s) 272
PstNI CAGNNNCTG 1 cut(s) 80
RsaI GTAC 2 cut(s) 13, 107
RsaNI GTAC 2 cut(s) 12, 106
RseI CAYNNNNRTG 1 cut(s) 299
SalI GTCGAC 1 cut(s) 117
SatI GCNGC 4 cut(s) 81, 84, 168, 344
Sau3AI GATC 1 cut(s) 185
Sau96I GGNCC 2 cut(s) 127, 272
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 1 cut(s) 195
SetI ASST 4 cut(s) 13, 65, 139, 214
SfaNI GCATC 2 cut(s) 61, 196
SfoI GGCGCC 1 cut(s) 179
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 299
Sse9I AATT 1 cut(s) 172
SsiI CCGC 1 cut(s) 344
SspDI GGCGCC 1 cut(s) 177
StyD4I CCNGG 1 cut(s) 145
StyI CCWWGG 1 cut(s) 275
TaaI ACNGT 1 cut(s) 123
TaiI ACGT 1 cut(s) 65
TaqI TCGA 2 cut(s) 39, 118
TasI AATT 1 cut(s) 172
TauI GCSGC 1 cut(s) 346
TfiI GAWTC 3 cut(s) 47, 150, 321
TscAI CASTG 1 cut(s) 252
TseI GCWGC 3 cut(s) 80, 83, 167
TspDTI ATGAA 1 cut(s) 254
TspRI CASTG 1 cut(s) 252
VpaK11BI GGWCC 1 cut(s) 272
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
XmiI GTMKAC 2 cut(s) 118, 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.