Rorug02G0460500

Glycine-rich protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001806
Physical Location & Seq
Forward (+)
59254239 .. 59254919
681 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0460500.1

Sequence Viewer

Length: 681 bp
ATGGTGTCTTCTGCACTTATATTCCAAAACGCCATCAAATCCATTTCTCAACAACAACAAAAGCATAAACCGAAGACCTTCTTTTATCAAACCCCAAATTCCATTTCATTCAAACCTCGATGTATGACTGTTACTTCCAAATATGTTCCTAGTATATCATCCATGGACACCAAGATTGGAAATGGAGAGCTAGAAGCGTCCAAAGTTATAACAAGCAGTGACGTCCGCGTGGCTTACTCCTCCTCCACTCTCCCGGTCACACTAGGTGGATACCTAGCTCGGCGACTCGTCCAAATCGGTGTGGCGGACGTGTTCTCTGTTCCCGGTGACTCTAACTTGGTCCTTTTAGATCATCTCATTGCCGAGCCCAGGCTAACCAATATTGGGTGCTGCAATGAGCTCAACGCCGGGTATGTTGCTGATGGCTACGCGCGCTCCTGCGGCGTCGGCGCGTGTGTCGTTACTTTCACCGTCGGCGGACTCAGTTTGCTGAATGCCATAGCCGGGGCGTACAGTGAGAACTTGCCGGTGATTTGTATAGTTGGAGGACCGAATTCCAATGACTATGGAGCCAAGTCGACTAACAATATTCTTCACCATACTATCGGGCTACCTGATTTCAGCCAAGAGCTAAGGTGCTTTCAGACCGTCACTTGCTATCAAGTTAGTACTGCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRP

Protein Analysis

226

Amino Acids

24.1

Weight (kDa)

8.05

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 87 - 195 3.1e-28 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 209
AatII GACGTC 1 cut(s) 225
AccI GTMKAC 1 cut(s) 578
AccII CGCG 4 cut(s) 228, 431, 433, 452
AciI CCGC 4 cut(s) 226, 305, 441, 477
AcsI RAATTY 2 cut(s) 97, 553
AcyI GRCGYC 2 cut(s) 222, 444
AdeI CACNNNGTG 1 cut(s) 266
AfaI GTAC 2 cut(s) 512, 670
AfiI CCNNNNNNNGG 3 cut(s) 369, 384, 504
AflIII ACRYGT 1 cut(s) 309
AgsI TTSAA 1 cut(s) 112
AjiI CACGTC 1 cut(s) 310
AjnI CCWGG 1 cut(s) 368
AjuI GAANNNNNNNTTGG 2 cut(s) 88, 120
AluBI AGCT 4 cut(s) 190, 278, 400, 631
AluI AGCT 4 cut(s) 190, 278, 400, 631
Alw21I GWGCWC 1 cut(s) 402
ApeKI GCWGC 1 cut(s) 390
ApoI RAATTY 2 cut(s) 97, 553
Asp700I GAANNNNTTC 1 cut(s) 77
AspLEI GCGC 3 cut(s) 433, 435, 452
AspS9I GGNCC 2 cut(s) 340, 548
AsuC2I CCSGG 4 cut(s) 254, 324, 409, 505
AsuHPI GGTGA 4 cut(s) 338, 460, 541, 587
AvaII GGWCC 2 cut(s) 340, 548
BanII GRGCYC 2 cut(s) 369, 402
BbsI GAAGAC 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 377
BccI CCATC 2 cut(s) 41, 416
BciT130I CCWGG 1 cut(s) 370
BciVI GTATCC 1 cut(s) 263
BcnI CCSGG 4 cut(s) 254, 324, 409, 505
BfaI CTAG 4 cut(s) 150, 191, 263, 275
BfuI GTATCC 1 cut(s) 263
BisI GCNGC 2 cut(s) 391, 442
BlsI GCNGC 2 cut(s) 392, 443
BmcAI AGTACT 1 cut(s) 670
Bme1390I CCNGG 5 cut(s) 254, 324, 370, 409, 505
Bme18I GGWCC 2 cut(s) 340, 548
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 2 cut(s) 340, 548
BmiI GGNNCC 1 cut(s) 571
BmrFI CCNGG 5 cut(s) 254, 324, 370, 409, 505
BpiI GAAGAC 1 cut(s) 80
Bpu10I CCTNAGC 1 cut(s) 632
BpuMI CCSGG 4 cut(s) 254, 324, 409, 505
BsaHI GRCGYC 2 cut(s) 222, 444
BsaJI CCNNGG 3 cut(s) 162, 368, 504
BsaXI ACNNNNNCTCC 4 cut(s) 227, 257, 419, 449
Bsc4I CCNNNNNNNGG 3 cut(s) 369, 384, 504
Bse118I RCCGGY 1 cut(s) 526
Bse3DI GCAATG 2 cut(s) 357, 400
BseBI CCWGG 1 cut(s) 370
BseDI CCNNGG 3 cut(s) 162, 368, 504
BseGI GGATG 1 cut(s) 158
BseLI CCNNNNNNNGG 3 cut(s) 369, 384, 504
BseMI GCAATG 2 cut(s) 357, 400
BseMII CTCAG 1 cut(s) 496
BsePI GCGCGC 1 cut(s) 431
BseRI GAGGAG 2 cut(s) 229, 232
BseXI GCAGC 1 cut(s) 377
Bsh1236I CGCG 4 cut(s) 228, 431, 433, 452
BsiHKAI GWGCWC 1 cut(s) 402
BsiSI CCGG 5 cut(s) 254, 324, 408, 504, 527
BslI CCNNNNNNNGG 3 cut(s) 369, 384, 504
BsmI GAATGC 1 cut(s) 499
Bsp1286I GDGCHC 2 cut(s) 369, 402
Bsp143I GATC 1 cut(s) 349
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 4 cut(s) 226, 305, 441, 477
BspCNI CTCAG 1 cut(s) 495
BspFNI CGCG 4 cut(s) 228, 431, 433, 452
BspLI GGNNCC 1 cut(s) 571
BsrDI GCAATG 2 cut(s) 357, 400
BsrFI RCCGGY 1 cut(s) 526
BssAI RCCGGY 1 cut(s) 526
BssECI CCNNGG 3 cut(s) 162, 368, 504
BssHII GCGCGC 1 cut(s) 431
BssMI GATC 1 cut(s) 349
BssNI GRCGYC 2 cut(s) 222, 444
BssT1I CCWWGG 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 370
Bst4CI ACNGT 4 cut(s) 130, 472, 515, 649
BstACI GRCGYC 2 cut(s) 222, 444
BstC8I GCNNGC 1 cut(s) 433
BstDEI CTNAG 2 cut(s) 482, 632
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 1 cut(s) 158
BstFNI CGCG 4 cut(s) 228, 431, 433, 452
BstHHI GCGC 3 cut(s) 433, 435, 452
BstKTI GATC 1 cut(s) 352
BstMBI GATC 1 cut(s) 349
BstMWI GCNNNNNNNGC 3 cut(s) 432, 441, 447
BstNI CCWGG 1 cut(s) 370
BstNSI RCATGY 1 cut(s) 678
BstSCI CCNGG 5 cut(s) 252, 322, 368, 407, 503
BstUI CGCG 4 cut(s) 228, 431, 433, 452
BstV1I GCAGC 1 cut(s) 377
BstV2I GAAGAC 1 cut(s) 80
BsuI GTATCC 1 cut(s) 263
BtgI CCRYGG 1 cut(s) 162
BtrI CACGTC 1 cut(s) 310
BtsCI GGATG 1 cut(s) 158
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 2 cut(s) 223, 520
Cac8I GCNNGC 1 cut(s) 433
CfoI GCGC 3 cut(s) 433, 435, 452
Cfr10I RCCGGY 1 cut(s) 526
Cfr13I GGNCC 2 cut(s) 340, 548
CseI GACGC 2 cut(s) 186, 433
Csp6I GTAC 2 cut(s) 511, 669
CviAII CATG 2 cut(s) 163, 675
CviQI GTAC 2 cut(s) 511, 669
DdeI CTNAG 2 cut(s) 482, 632
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
DraIII CACNNNGTG 1 cut(s) 266
EciI GGCGGA 2 cut(s) 320, 492
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 162
Eco24I GRGCYC 2 cut(s) 369, 402
Eco47I GGWCC 2 cut(s) 340, 548
Eco53kI GAGCTC 1 cut(s) 400
EcoICRI GAGCTC 1 cut(s) 400
EcoRI GAATTC 1 cut(s) 553
EcoRII CCWGG 1 cut(s) 368
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 2 cut(s) 369, 402
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 2 cut(s) 166, 678
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FatI CATG 2 cut(s) 162, 674
FblI GTMKAC 1 cut(s) 578
Fnu4HI GCNGC 2 cut(s) 391, 442
FokI GGATG 1 cut(s) 145
FriOI GRGCYC 2 cut(s) 369, 402
Fsp4HI GCNGC 2 cut(s) 391, 442
FspBI CTAG 4 cut(s) 150, 191, 263, 275
GlaI GCGC 3 cut(s) 432, 434, 451
GluI GCNGC 2 cut(s) 391, 442
HapII CCGG 5 cut(s) 254, 324, 408, 504, 527
HgaI GACGC 2 cut(s) 186, 433
HhaI GCGC 3 cut(s) 433, 435, 452
Hin1I GRCGYC 2 cut(s) 222, 444
Hin1II CATG 2 cut(s) 166, 678
Hin6I GCGC 3 cut(s) 431, 433, 450
HinP1I GCGC 3 cut(s) 431, 433, 450
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HinfI GANTC 3 cut(s) 285, 329, 480
HpaII CCGG 5 cut(s) 254, 324, 408, 504, 527
HphI GGTGA 4 cut(s) 338, 460, 541, 587
Hpy166II GTNNAC 1 cut(s) 579
Hpy188I TCNGA 1 cut(s) 645
Hpy8I GTNNAC 1 cut(s) 579
Hpy99I CGWCG 2 cut(s) 449, 476
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 4 cut(s) 130, 472, 515, 649
HpyCH4IV ACGT 2 cut(s) 222, 309
HpyCH4V TGCA 3 cut(s) 14, 393, 674
HpyF10VI GCNNNNNNNGC 3 cut(s) 432, 441, 447
HpyF3I CTNAG 2 cut(s) 482, 632
HpySE526I ACGT 2 cut(s) 222, 309
Hsp92I GRCGYC 2 cut(s) 222, 444
Hsp92II CATG 2 cut(s) 166, 678
HspAI GCGC 3 cut(s) 431, 433, 450
Kzo9I GATC 1 cut(s) 349
LmnI GCTCC 2 cut(s) 440, 569
LpnPI CCDG 9 cut(s) 267, 337, 355, 382, 421, 451, 517, 540, 627
Lsp1109I GCAGC 1 cut(s) 377
MaeI CTAG 4 cut(s) 150, 191, 263, 275
MaeII ACGT 2 cut(s) 222, 309
MaeIII GTNAC 6 cut(s) 130, 218, 256, 326, 460, 649
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MboII GAAGA 2 cut(s) 85, 584
MhlI GDGCHC 2 cut(s) 369, 402
MluCI AATT 2 cut(s) 97, 553
MlyI GAGTC 3 cut(s) 279, 323, 474
MmeI TCCRAC 1 cut(s) 523
MnlI CCTC 4 cut(s) 126, 250, 253, 539
MroXI GAANNNNTTC 1 cut(s) 77
MseI TTAA 1 cut(s) 679
MspI CCGG 5 cut(s) 254, 324, 408, 504, 527
MspR9I CCNGG 5 cut(s) 254, 324, 370, 409, 505
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 1 cut(s) 370
MvnI CGCG 4 cut(s) 228, 431, 433, 452
MwoI GCNNNNNNNGC 3 cut(s) 432, 441, 447
NciI CCSGG 4 cut(s) 254, 324, 409, 505
NcoI CCATGG 1 cut(s) 162
NdeII GATC 1 cut(s) 349
NlaIII CATG 2 cut(s) 166, 678
NlaIV GGNNCC 1 cut(s) 571
NmeAIII GCCGAG 2 cut(s) 259, 388
NmuCI GTSAC 4 cut(s) 218, 256, 326, 649
NspI RCATGY 1 cut(s) 678
PauI GCGCGC 1 cut(s) 431
PctI GAATGC 1 cut(s) 499
PdmI GAANNNNTTC 1 cut(s) 77
PkrI GCNGC 2 cut(s) 392, 443
PleI GAGTC 3 cut(s) 279, 323, 474
PpsI GAGTC 3 cut(s) 279, 323, 474
PsiI TTATAA 1 cut(s) 209
Psp124BI GAGCTC 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 368
PspGI CCWGG 1 cut(s) 368
PspN4I GGNNCC 1 cut(s) 571
PspPI GGNCC 2 cut(s) 340, 548
PteI GCGCGC 1 cut(s) 431
RsaI GTAC 2 cut(s) 512, 670
RsaNI GTAC 2 cut(s) 511, 669
SacI GAGCTC 1 cut(s) 402
SalI GTCGAC 1 cut(s) 577
SaqAI TTAA 1 cut(s) 679
SatI GCNGC 2 cut(s) 391, 442
Sau3AI GATC 1 cut(s) 349
Sau96I GGNCC 2 cut(s) 340, 548
ScaI AGTACT 1 cut(s) 670
SchI GAGTC 3 cut(s) 279, 323, 474
ScrFI CCNGG 5 cut(s) 254, 324, 370, 409, 505
SduI GDGCHC 2 cut(s) 369, 402
SinI GGWCC 2 cut(s) 340, 548
Sse9I AATT 2 cut(s) 97, 553
SsiI CCGC 4 cut(s) 226, 305, 441, 477
SspI AATATT 2 cut(s) 382, 589
SspMI CTAG 4 cut(s) 150, 191, 263, 275
SstI GAGCTC 1 cut(s) 402
StyD4I CCNGG 5 cut(s) 252, 322, 368, 407, 503
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 4 cut(s) 130, 472, 515, 649
TaiI ACGT 2 cut(s) 225, 312
TaqI TCGA 2 cut(s) 118, 578
TaqII GACCGA 1 cut(s) 565
TasI AATT 2 cut(s) 97, 553
TatI WGTACW 1 cut(s) 668
TauI GCSGC 1 cut(s) 444
Tru1I TTAA 1 cut(s) 679
Tru9I TTAA 1 cut(s) 679
TscAI CASTG 2 cut(s) 223, 520
TseFI GTSAC 4 cut(s) 218, 256, 326, 649
TseI GCWGC 1 cut(s) 390
Tsp45I GTSAC 4 cut(s) 218, 256, 326, 649
TspDTI ATGAA 1 cut(s) 96
TspRI CASTG 2 cut(s) 223, 520
VpaK11BI GGWCC 2 cut(s) 340, 548
XapI RAATTY 2 cut(s) 97, 553
XceI RCATGY 1 cut(s) 678
XmiI GTMKAC 1 cut(s) 578
XmnI GAANNNNTTC 1 cut(s) 77
XspI CTAG 4 cut(s) 150, 191, 263, 275
ZraI GACGTC 1 cut(s) 223
ZrmI AGTACT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.