Rorug02G0632300

Heparan-alpha-glucosaminide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006954
Physical Location & Seq
Forward (+)
74697401 .. 74698231
831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0632300.1

Sequence Viewer

Length: 432 bp
ATGTCACTCATTTCCCAACTGTTTGGGGATGAAACATTGGATCCCTTCCTTTCTATGATCAACCAGTGTCCAGTGCTCAGCACCCCGACTGATTGGAAGGAGACCCCTAAAGCCCACGTTTTCGTTTCCGACCTGCCAGGGCTGAAGAAAGAGGAGGTGAAAGTGGAGGTTGACGATGGCAGAGTGCTCCAAATAAGTGGCGAGAGGAAAGTAGAAGAAGAAAATGGTGGCGGTGATAAGAATGATCGTCAGTATAAGTGGCACCGTGTCGAACGTTGCCGTGGCAAGTTCCTCAGGAGGTTTAAGCTACCGGAAAATGCCAAGACTGATGACATTCAGGCGTCCATGGAGAACGGAGTGCTTACTGTCACTGTTCCCAAGCAGGAGGTTCCAAAACCTCAAAGGAAGGTCGTTGAGATTGAAGCACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
DUF1624 DUF5009

Protein Analysis

143

Amino Acids

16.34

Weight (kDa)

5.97

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 31 - 140 2.5e-27 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 141
AccB1I GGYRCC 1 cut(s) 261
AciI CCGC 1 cut(s) 231
AclI AACGTT 1 cut(s) 274
AclWI GGATC 2 cut(s) 35, 48
AcuI CTGAAG 1 cut(s) 164
AcyI GRCGYC 1 cut(s) 341
AgsI TTSAA 1 cut(s) 422
AjnI CCWGG 1 cut(s) 136
AluBI AGCT 1 cut(s) 307
AluI AGCT 1 cut(s) 307
Alw21I GWGCWC 2 cut(s) 78, 189
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 2 cut(s) 35, 48
AsuHPI GGTGA 2 cut(s) 169, 245
AxyI CCTNAGG 1 cut(s) 293
BamHI GGATCC 1 cut(s) 40
BanI GGYRCC 1 cut(s) 261
Bbv12I GWGCWC 2 cut(s) 78, 189
BccI CCATC 1 cut(s) 170
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 1 cut(s) 138
BclI TGATCA 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 95
BfuAI ACCTGC 1 cut(s) 141
BlpI GCTNAGC 1 cut(s) 77
Bme1390I CCNGG 1 cut(s) 138
BmiI GGNNCC 3 cut(s) 42, 263, 390
BmrFI CCNGG 1 cut(s) 138
Bpu1102I GCTNAGC 1 cut(s) 77
BsaHI GRCGYC 1 cut(s) 341
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 137, 280, 345
BsaWI WCCGGW 1 cut(s) 310
Bse1I ACTGG 2 cut(s) 64, 71
Bse21I CCTNAGG 1 cut(s) 293
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 3 cut(s) 137, 280, 345
BseGI GGATG 1 cut(s) 34
BseMII CTCAG 2 cut(s) 91, 307
BseNI ACTGG 2 cut(s) 64, 71
BseRI GAGGAG 1 cut(s) 167
BshNI GGYRCC 1 cut(s) 261
BsiHKAI GWGCWC 2 cut(s) 78, 189
BsiSI CCGG 1 cut(s) 311
BsmAI GTCTC 1 cut(s) 95
Bso31I GGTCTC 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 78, 189
Bsp143I GATC 3 cut(s) 40, 57, 244
Bsp1720I GCTNAGC 1 cut(s) 77
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 1 cut(s) 231
BspCNI CTCAG 2 cut(s) 90, 306
BspLI GGNNCC 3 cut(s) 42, 263, 390
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 2 cut(s) 35, 48
BspT107I GGYRCC 1 cut(s) 261
BspTNI GGTCTC 1 cut(s) 95
BsrI ACTGG 2 cut(s) 64, 71
BssECI CCNNGG 3 cut(s) 137, 280, 345
BssMI GATC 3 cut(s) 40, 57, 244
BssNI GRCGYC 1 cut(s) 341
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 4 cut(s) 21, 266, 367, 373
BstACI GRCGYC 1 cut(s) 341
BstDEI CTNAG 2 cut(s) 77, 293
BstDSI CCRYGG 2 cut(s) 280, 345
BstF5I GGATG 1 cut(s) 34
BstKTI GATC 3 cut(s) 43, 60, 247
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 3 cut(s) 40, 57, 244
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstX2I RGATCY 1 cut(s) 40
BstXI CCANNNNNNTGG 2 cut(s) 23, 197
BstYI RGATCY 1 cut(s) 40
Bsu36I CCTNAGG 1 cut(s) 293
BtgI CCRYGG 2 cut(s) 280, 345
BtsCI GGATG 1 cut(s) 34
BtsIMutI CAGTG 3 cut(s) 71, 78, 369
BveI ACCTGC 1 cut(s) 141
CseI GACGC 1 cut(s) 330
CviAII CATG 1 cut(s) 346
CviJI RGCY 3 cut(s) 113, 142, 307
CviKI_1 RGCY 3 cut(s) 113, 142, 307
DdeI CTNAG 2 cut(s) 77, 293
DpnI GATC 3 cut(s) 42, 59, 246
DpnII GATC 3 cut(s) 40, 57, 244
Eco130I CCWWGG 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 164
Eco81I CCTNAGG 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 1 cut(s) 349
FaiI YATR 3 cut(s) 56, 255, 347
FatI CATG 1 cut(s) 345
FbaI TGATCA 1 cut(s) 57
FokI GGATG 1 cut(s) 41
HapII CCGG 1 cut(s) 311
HgaI GACGC 1 cut(s) 330
Hin1I GRCGYC 1 cut(s) 341
Hin1II CATG 1 cut(s) 349
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HpaII CCGG 1 cut(s) 311
HphI GGTGA 2 cut(s) 169, 245
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 1 cut(s) 295
Hpy8I GTNNAC 1 cut(s) 172
HpyAV CCTTC 3 cut(s) 55, 91, 400
HpyCH4III ACNGT 4 cut(s) 21, 266, 367, 373
HpyCH4IV ACGT 2 cut(s) 117, 274
HpyF3I CTNAG 2 cut(s) 77, 293
HpySE526I ACGT 2 cut(s) 117, 274
Hsp92I GRCGYC 1 cut(s) 341
Hsp92II CATG 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 57
Kzo9I GATC 3 cut(s) 40, 57, 244
LmnI GCTCC 1 cut(s) 192
LpnPI CCDG 9 cut(s) 77, 84, 123, 146, 150, 280, 323, 324, 368
MaeII ACGT 2 cut(s) 117, 274
MaeIII GTNAC 2 cut(s) 3, 367
MalI GATC 3 cut(s) 42, 59, 246
MboI GATC 3 cut(s) 40, 57, 244
MboII GAAGA 3 cut(s) 157, 227, 230
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 2 cut(s) 78, 189
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 8 cut(s) 145, 148, 160, 198, 291, 302, 379, 408
MseI TTAA 2 cut(s) 303, 430
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 138
NcoI CCATGG 1 cut(s) 345
NdeII GATC 3 cut(s) 40, 57, 244
NlaIII CATG 1 cut(s) 349
NlaIV GGNNCC 3 cut(s) 42, 263, 390
NmuCI GTSAC 2 cut(s) 3, 367
Psp1406I AACGTT 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 3 cut(s) 42, 263, 390
PsuI RGATCY 1 cut(s) 40
SaqAI TTAA 2 cut(s) 303, 430
Sau3AI GATC 3 cut(s) 40, 57, 244
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 2 cut(s) 78, 189
SsiI CCGC 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 4 cut(s) 21, 266, 367, 373
TaiI ACGT 2 cut(s) 120, 277
TaqI TCGA 1 cut(s) 270
Tru1I TTAA 2 cut(s) 303, 430
Tru9I TTAA 2 cut(s) 303, 430
TscAI CASTG 3 cut(s) 71, 78, 376
TseFI GTSAC 2 cut(s) 3, 367
Tsp45I GTSAC 2 cut(s) 3, 367
TspDTI ATGAA 1 cut(s) 45
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 3 cut(s) 71, 78, 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.