Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0009679

Physical Location & Seq

Genomic Coordinates Forward (+)

24052580 .. 24053540

Gene Structure 961 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0144700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 861 bp

ATGGGTTCACCTGCTTTCTCTTGTGCAATACCGAAACACATGCTGAAGCAGCAGCTTGTCATTGTGGCTGGAATAGCTCTGGTGCTAGTCCTTAGTGTACATGTAGTAGAGGGTCAAAATTGTAGTTGTGCTGCAGACCTGTGCTGCAGCCGATACGGCTACTGCGGCACAGGCGATGACTACTGCGGGACGGGATGCCAAGCGGGGCCTTGCAACACGGCGCCTCTAACTCCCAGTACCAGTGATGTTCCAGTGGCCGACATCGTGACCCCGACATTCTTCAACGGGACAATTGATCAAGCGGAAGCAAGCTGTGCCGGGAAGGACTTTTATTCTCGGGCAACGTTCCTTGATGCTCTCGAGTTGTATGATCAGTTTGGTAAGATTGGTTCCCTTGATGATTCTAAACGGGAGATTGCTGCCTTCTTTGCCCACGTCACCCATGAGACTGGACATTTTTGCTACATAGAAGAGATAGACGGAGCAACAAAAGACTACTGCGACGAGACTAACACGGAGTATCCATGCAACCCGAACAAAGGTTATTACGGCCGGGGACCCATCCAACTATCGTGGAACTTCAATTACGGTCCGGCCGGAGAGAGTATTGGGTTCGACGGACTAAACTCCCCTGAAACCGTGGCCAATGACCCACTTATTGCTTTTAAGACTGCACTATGGTACTGGATGAACAATGTTCGGCCGGTCATTGCTGAAGGATTCGGGGAAACTATCCGAGCCATTAACGGTGCGCTCGAATGCGATGGTGGAAACCCTGCTACTGTACAGAAACGCGTCGACTACTACATTGATTATTGTAACCAACTTGGTGTTGCTCCTGGTGACAACCTTACTTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000079 GO:0000307 GO:0001932 GO:0001934 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0016538 GO:0019207 GO:0019219 GO:0019220 GO:0019222 GO:0019887 GO:0030234 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032991 GO:0033674 GO:0042325 GO:0042327 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043549 GO:0044093 GO:0044424 GO:0044464 GO:0045737 GO:0045787 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051726 GO:0060255 GO:0061695 GO:0065007 GO:0065009 GO:0071900 GO:0071902 GO:0080090 GO:0098772 GO:1901407 GO:1901409 GO:1902494 GO:1902554 GO:1902680 GO:1902911 GO:1903506 GO:1903508 GO:1904029 GO:1904031 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko05202 map05202

Pfam Domains

Protein Families

Cyclin_N

Protein Analysis

286

Amino Acids

30.8

Weight (kDa)

4.43

Isoelectric Point (pI)

30.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Chitin_bind_1	PF00187	39 - 71	2.6e-08	Chitin recognition protein
Glyco_hydro_19	PF00182	93 - 233	3.2e-37	Chitinase class I
Glyco_hydro_19	PF00182	239 - 286	6.4e-09	Chitinase class I

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009679)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g15100 FvH4_4g15100
malus_domestica	MD13G1188600.v1.1 MD16G1189200.v1.1
prunus_persica	Prupe.1G155700_v2.0.a1 Prupe.1G155700_v2.0.a1 Prupe.1G155700_v2.0.a1 Prupe.1G155700_v2.0.a1
pyrus_communis	pycom13g16300 pycom16g15920
rosa_chinensis	RchiOBHm_Chr4g0416761
rosa_laevigata	RLG00000007953
rosa_multiflora	Rmu_sc0036474.1_g000001
rosa_roxburghii	Rroxscaffold_5G00360640
rosa_rugosa	Rorug04G0144600 Rorug04G0144700 Rorug04G0144800
rosa_samantha	Rh4AG207300 Rh4BG204500 Rh4CG218300 Rh4DG204400
rosa_wichuraiana	Rw4G017530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	19
Acc36I	ACCTGC	1 cut(s)	19
AccB1I	GGYRCC	1 cut(s)	220
AccI	GTMKAC	1 cut(s)	798
AccII	CGCG	1 cut(s)	795
AciI	CCGC	4 cut(s)	165, 186, 203, 302
AclI	AACGTT	1 cut(s)	344
AcoI	YGGCCR	5 cut(s)	255, 550, 594, 642, 701
AcuI	CTGAAG	2 cut(s)	65, 735
AcyI	GRCGYC	1 cut(s)	221
AfaI	GTAC	4 cut(s)	99, 238, 683, 786
AfiI	CCNNNNNNNGG	1 cut(s)	539
AflIII	ACRYGT	2 cut(s)	100, 793
AgsI	TTSAA	2 cut(s)	283, 583
AjiI	CACGTC	1 cut(s)	436
AjnI	CCWGG	1 cut(s)	838
AluBI	AGCT	3 cut(s)	55, 77, 312
AluI	AGCT	3 cut(s)	55, 77, 312
Alw26I	GTCTC	2 cut(s)	440, 500
Ama87I	CYCGRG	2 cut(s)	336, 359
AoxI	GGCC	6 cut(s)	206, 255, 550, 594, 642, 701
ApeKI	GCWGC	6 cut(s)	49, 52, 131, 144, 147, 419
AspLEI	GCGC	2 cut(s)	223, 754
AspS9I	GGNCC	3 cut(s)	206, 557, 590
AsuC2I	CCSGG	2 cut(s)	319, 554
AsuHPI	GGTGA	2 cut(s)	430, 854
AvaI	CYCGRG	2 cut(s)	336, 359
AvaII	GGWCC	2 cut(s)	557, 590
BalI	TGGCCA	1 cut(s)	644
BanI	GGYRCC	1 cut(s)	220
BbvI	GCAGC	6 cut(s)	61, 64, 118, 131, 159, 406
BccI	CCATC	2 cut(s)	569, 758
BceAI	ACGGC	3 cut(s)	172, 234, 565
BcgI	CGANNNNNNTGC	2 cut(s)	22, 56
BciT130I	CCWGG	1 cut(s)	840
BciVI	GTATCC	1 cut(s)	531
BclI	TGATCA	2 cut(s)	295, 370
BcnI	CCSGG	2 cut(s)	319, 554
BcoDI	GTCTC	2 cut(s)	440, 500
BfaI	CTAG	2 cut(s)	86, 859
BfmI	CTRYAG	2 cut(s)	132, 145
BfoI	RGCGCY	1 cut(s)	224
BfuAI	ACCTGC	1 cut(s)	19
BfuI	GTATCC	1 cut(s)	531
BglI	GCCNNNNNGGC	1 cut(s)	156
BisI	GCNGC	7 cut(s)	50, 53, 132, 145, 148, 166, 420
BlsI	GCNGC	7 cut(s)	51, 54, 133, 146, 149, 167, 421
Bme1390I	CCNGG	3 cut(s)	319, 554, 840
Bme18I	GGWCC	2 cut(s)	557, 590
BmeT110I	CYCGRG	2 cut(s)	336, 359
BmgBI	CACGTC	1 cut(s)	436
BmgT120I	GGNCC	3 cut(s)	206, 557, 590
BmiI	GGNNCC	5 cut(s)	207, 222, 391, 558, 559
BmrFI	CCNGG	3 cut(s)	319, 554, 840
BmrI	ACTGGG	1 cut(s)	228
BmsI	GCATC	2 cut(s)	185, 343
BmuI	ACTGGG	1 cut(s)	228
BpuMI	CCSGG	2 cut(s)	319, 554
BsaHI	GRCGYC	1 cut(s)	221
BsaJI	CCNNGG	2 cut(s)	553, 639
Bsc4I	CCNNNNNNNGG	1 cut(s)	539
Bse118I	RCCGGY	1 cut(s)	703
Bse1I	ACTGG	5 cut(s)	234, 240, 251, 454, 689
Bse3DI	GCAATG	1 cut(s)	708
BseBI	CCWGG	1 cut(s)	840
BseDI	CCNNGG	2 cut(s)	553, 639
BseGI	GGATG	3 cut(s)	200, 561, 693
BseLI	CCNNNNNNNGG	1 cut(s)	539
BseMI	GCAATG	1 cut(s)	708
BseNI	ACTGG	5 cut(s)	234, 240, 251, 454, 689
BseX3I	CGGCCG	3 cut(s)	550, 594, 701
BseXI	GCAGC	6 cut(s)	61, 64, 118, 131, 159, 406
BsgI	GTGCAG	1 cut(s)	657
Bsh1236I	CGCG	1 cut(s)	795
Bsh1285I	CGRYCG	3 cut(s)	553, 597, 704
BshFI	GGCC	6 cut(s)	208, 257, 552, 596, 644, 703
BshNI	GGYRCC	1 cut(s)	220
BsiEI	CGRYCG	3 cut(s)	553, 597, 704
BsiHKCI	CYCGRG	2 cut(s)	336, 359
BsiSI	CCGG	5 cut(s)	318, 553, 593, 597, 704
BslFI	GGGAC	3 cut(s)	202, 301, 570
BslI	CCNNNNNNNGG	1 cut(s)	539
BsmAI	GTCTC	2 cut(s)	440, 500
BsmFI	GGGAC	3 cut(s)	202, 301, 570
BsmI	GAATGC	1 cut(s)	764
BsnI	GGCC	6 cut(s)	208, 257, 552, 596, 644, 703
BsoBI	CYCGRG	2 cut(s)	336, 359
Bsp1407I	TGTACA	2 cut(s)	97, 784
Bsp143I	GATC	2 cut(s)	295, 370
BspACI	CCGC	4 cut(s)	165, 186, 203, 302
BspANI	GGCC	6 cut(s)	208, 257, 552, 596, 644, 703
BspFNI	CGCG	1 cut(s)	795
BspLI	GGNNCC	5 cut(s)	207, 222, 391, 558, 559
BspMAI	CTGCAG	2 cut(s)	136, 149
BspMI	ACCTGC	1 cut(s)	19
BspT107I	GGYRCC	1 cut(s)	220
BsrDI	GCAATG	1 cut(s)	708
BsrFI	RCCGGY	1 cut(s)	703
BsrGI	TGTACA	2 cut(s)	97, 784
BsrI	ACTGG	5 cut(s)	234, 240, 251, 454, 689
BssAI	RCCGGY	1 cut(s)	703
BssECI	CCNNGG	2 cut(s)	553, 639
BssMI	GATC	2 cut(s)	295, 370
BssNI	GRCGYC	1 cut(s)	221
Bst2UI	CCWGG	1 cut(s)	840
Bst4CI	ACNGT	4 cut(s)	590, 640, 749, 784
Bst6I	CTCTTC	1 cut(s)	465
BstACI	GRCGYC	1 cut(s)	221
BstAPI	GCANNNNNTGC	1 cut(s)	314
BstAUI	TGTACA	2 cut(s)	97, 784
BstC8I	GCNNGC	1 cut(s)	310
BstDEI	CTNAG	1 cut(s)	92
BstDSI	CCRYGG	1 cut(s)	639
BstF5I	GGATG	3 cut(s)	200, 561, 693
BstFNI	CGCG	1 cut(s)	795
BstH2I	RGCGCY	1 cut(s)	224
BstHHI	GCGC	2 cut(s)	223, 754
BstKTI	GATC	2 cut(s)	298, 373
BstMAI	GTCTC	2 cut(s)	440, 500
BstMBI	GATC	2 cut(s)	295, 370
BstMCI	CGRYCG	3 cut(s)	553, 597, 704
BstMWI	GCNNNNNNNGC	7 cut(s)	49, 74, 156, 165, 171, 314, 428
BstNI	CCWGG	1 cut(s)	840
BstNSI	RCATGY	2 cut(s)	43, 104
BstSCI	CCNGG	3 cut(s)	317, 552, 838
BstSFI	CTRYAG	2 cut(s)	132, 145
BstUI	CGCG	1 cut(s)	795
BstV1I	GCAGC	6 cut(s)	61, 64, 118, 131, 159, 406
BstXI	CCANNNNNNTGG	1 cut(s)	449
BstZI	CGGCCG	3 cut(s)	550, 594, 701
BsuI	GTATCC	1 cut(s)	531
BsuRI	GGCC	6 cut(s)	208, 257, 552, 596, 644, 703
BtgI	CCRYGG	1 cut(s)	639
BtgZI	GCGATG	2 cut(s)	189, 777
BtrI	CACGTC	1 cut(s)	436
BtsCI	GGATG	3 cut(s)	200, 561, 693
BtsIMutI	CAGTG	2 cut(s)	247, 258
BveI	ACCTGC	1 cut(s)	19
Cac8I	GCNNGC	1 cut(s)	310
CfoI	GCGC	2 cut(s)	223, 754
Cfr10I	RCCGGY	1 cut(s)	703
Cfr13I	GGNCC	3 cut(s)	206, 557, 590
CpoI	CGGWCCG	1 cut(s)	590
CseI	GACGC	1 cut(s)	784
Csp6I	GTAC	4 cut(s)	98, 237, 682, 785
CspCI	CAANNNNNGTGG	2 cut(s)	554, 589
CspI	CGGWCCG	1 cut(s)	590
CviAII	CATG	4 cut(s)	40, 101, 443, 525
CviQI	GTAC	4 cut(s)	98, 237, 682, 785
DdeI	CTNAG	1 cut(s)	92
DinI	GGCGCC	1 cut(s)	222
DpnI	GATC	2 cut(s)	297, 372
DpnII	GATC	2 cut(s)	295, 370
EaeI	YGGCCR	5 cut(s)	255, 550, 594, 642, 701
EagI	CGGCCG	3 cut(s)	550, 594, 701
Eam1104I	CTCTTC	1 cut(s)	465
EarI	CTCTTC	1 cut(s)	465
EclXI	CGGCCG	3 cut(s)	550, 594, 701
Eco47I	GGWCC	2 cut(s)	557, 590
Eco52I	CGGCCG	3 cut(s)	550, 594, 701
Eco57I	CTGAAG	2 cut(s)	65, 735
Eco88I	CYCGRG	2 cut(s)	336, 359
EcoO109I	RGGNCCY	2 cut(s)	206, 557
EcoRII	CCWGG	1 cut(s)	838
EgeI	GGCGCC	1 cut(s)	222
EheI	GGCGCC	1 cut(s)	222
FaeI	CATG	4 cut(s)	43, 104, 446, 528
FaiI	YATR	7 cut(s)	41, 102, 369, 444, 467, 526, 679
FaqI	GGGAC	3 cut(s)	202, 301, 570
FatI	CATG	4 cut(s)	39, 100, 442, 524
FauI	CCCGC	2 cut(s)	179, 196
FbaI	TGATCA	2 cut(s)	295, 370
FblI	GTMKAC	1 cut(s)	798
Fnu4HI	GCNGC	7 cut(s)	50, 53, 132, 145, 148, 166, 420
FokI	GGATG	3 cut(s)	207, 548, 700
Fsp4HI	GCNGC	7 cut(s)	50, 53, 132, 145, 148, 166, 420
FspBI	CTAG	2 cut(s)	86, 859
GlaI	GCGC	2 cut(s)	222, 753
GluI	GCNGC	7 cut(s)	50, 53, 132, 145, 148, 166, 420
HaeII	RGCGCY	1 cut(s)	224
HaeIII	GGCC	6 cut(s)	208, 257, 552, 596, 644, 703
HapII	CCGG	5 cut(s)	318, 553, 593, 597, 704
HgaI	GACGC	1 cut(s)	784
HhaI	GCGC	2 cut(s)	223, 754
Hin1I	GRCGYC	1 cut(s)	221
Hin1II	CATG	4 cut(s)	43, 104, 446, 528
Hin6I	GCGC	2 cut(s)	221, 752
HinP1I	GCGC	2 cut(s)	221, 752
HincII	GTYRAC	1 cut(s)	799
HindII	GTYRAC	1 cut(s)	799
HinfI	GANTC	2 cut(s)	401, 720
HpaII	CCGG	5 cut(s)	318, 553, 593, 597, 704
HphI	GGTGA	2 cut(s)	430, 854
Hpy166II	GTNNAC	3 cut(s)	8, 98, 799
Hpy188I	TCNGA	1 cut(s)	737
Hpy188III	TCNNGA	2 cut(s)	265, 359
Hpy8I	GTNNAC	3 cut(s)	8, 98, 799
Hpy99I	CGWCG	3 cut(s)	506, 620, 800
HpyAV	CCTTC	3 cut(s)	316, 433, 710
HpyCH4III	ACNGT	4 cut(s)	590, 640, 749, 784
HpyCH4IV	ACGT	2 cut(s)	344, 435
HpyCH4V	TGCA	6 cut(s)	26, 134, 147, 213, 528, 674
HpyF10VI	GCNNNNNNNGC	7 cut(s)	49, 74, 156, 165, 171, 314, 428
HpyF3I	CTNAG	1 cut(s)	92
HpySE526I	ACGT	2 cut(s)	344, 435
Hsp92I	GRCGYC	1 cut(s)	221
Hsp92II	CATG	4 cut(s)	43, 104, 446, 528
HspAI	GCGC	2 cut(s)	221, 752
KasI	GGCGCC	1 cut(s)	220
KflI	GGGWCCC	1 cut(s)	557
Ksp22I	TGATCA	2 cut(s)	295, 370
Kzo9I	GATC	2 cut(s)	295, 370
LmnI	GCTCC	2 cut(s)	482, 841
Lsp1109I	GCAGC	6 cut(s)	61, 64, 118, 131, 159, 406
LweI	GCATC	2 cut(s)	185, 343
MaeI	CTAG	2 cut(s)	86, 859
MaeII	ACGT	2 cut(s)	344, 435
MaeIII	GTNAC	4 cut(s)	265, 436, 818, 842
MalI	GATC	2 cut(s)	297, 372
MboI	GATC	2 cut(s)	295, 370
MboII	GAAGA	2 cut(s)	271, 482
MfeI	CAATTG	1 cut(s)	291
MlsI	TGGCCA	1 cut(s)	644
MluCI	AATT	3 cut(s)	118, 291, 583
MluI	ACGCGT	1 cut(s)	793
MluNI	TGGCCA	1 cut(s)	644
Mly113I	GGCGCC	1 cut(s)	221
MmeI	TCCRAC	1 cut(s)	589
MnlI	CCTC	2 cut(s)	103, 234
Mox20I	TGGCCA	1 cut(s)	644
MscI	TGGCCA	1 cut(s)	644
MseI	TTAA	2 cut(s)	666, 744
Msp20I	TGGCCA	1 cut(s)	644
MspI	CCGG	5 cut(s)	318, 553, 593, 597, 704
MspR9I	CCNGG	3 cut(s)	319, 554, 840
MunI	CAATTG	1 cut(s)	291
Mva1269I	GAATGC	1 cut(s)	764
MvaI	CCWGG	1 cut(s)	840
MvnI	CGCG	1 cut(s)	795
MwoI	GCNNNNNNNGC	7 cut(s)	49, 74, 156, 165, 171, 314, 428
NarI	GGCGCC	1 cut(s)	221
NciI	CCSGG	2 cut(s)	319, 554
NdeII	GATC	2 cut(s)	295, 370
NlaIII	CATG	4 cut(s)	43, 104, 446, 528
NlaIV	GGNNCC	5 cut(s)	207, 222, 391, 558, 559
NmuCI	GTSAC	3 cut(s)	265, 436, 842
NspI	RCATGY	2 cut(s)	43, 104
PaeR7I	CTCGAG	1 cut(s)	359
PaqCI	CACCTGC	1 cut(s)	19
PciI	ACATGT	1 cut(s)	100
PcsI	WCGNNNNNNNCGW	1 cut(s)	753
PctI	GAATGC	1 cut(s)	764
PfeI	GAWTC	2 cut(s)	401, 720
PkrI	GCNGC	7 cut(s)	51, 54, 133, 146, 149, 167, 421
PluTI	GGCGCC	1 cut(s)	224
PpuMI	RGGWCCY	1 cut(s)	557
PscI	ACATGT	1 cut(s)	100
Psp1406I	AACGTT	1 cut(s)	344
Psp5II	RGGWCCY	1 cut(s)	557
Psp6I	CCWGG	1 cut(s)	838
PspGI	CCWGG	1 cut(s)	838
PspN4I	GGNNCC	5 cut(s)	207, 222, 391, 558, 559
PspPI	GGNCC	3 cut(s)	206, 557, 590
PspPPI	RGGWCCY	1 cut(s)	557
PsrI	GAACNNNNNNTAC	4 cut(s)	373, 405, 569, 601
PstI	CTGCAG	2 cut(s)	136, 149
RsaI	GTAC	4 cut(s)	99, 238, 683, 786
RsaNI	GTAC	4 cut(s)	98, 237, 682, 785
Rsr2I	CGGWCCG	1 cut(s)	590
RsrII	CGGWCCG	1 cut(s)	590
SalI	GTCGAC	1 cut(s)	797
SaqAI	TTAA	2 cut(s)	666, 744
SatI	GCNGC	7 cut(s)	50, 53, 132, 145, 148, 166, 420
Sau3AI	GATC	2 cut(s)	295, 370
Sau96I	GGNCC	3 cut(s)	206, 557, 590
ScrFI	CCNGG	3 cut(s)	319, 554, 840
SetI	ASST	9 cut(s)	13, 57, 79, 141, 314, 347, 438, 544, 852
SfaNI	GCATC	2 cut(s)	185, 343
SfcI	CTRYAG	2 cut(s)	132, 145
SfoI	GGCGCC	1 cut(s)	222
Sfr274I	CTCGAG	1 cut(s)	359
SinI	GGWCC	2 cut(s)	557, 590
SlaI	CTCGAG	1 cut(s)	359
SmlI	CTYRAG	1 cut(s)	359
SmoI	CTYRAG	1 cut(s)	359
Sse9I	AATT	3 cut(s)	118, 291, 583
SsiI	CCGC	4 cut(s)	165, 186, 203, 302
SspDI	GGCGCC	1 cut(s)	220
SspMI	CTAG	2 cut(s)	86, 859
StyD4I	CCNGG	3 cut(s)	317, 552, 838
TaaI	ACNGT	4 cut(s)	590, 640, 749, 784
TaiI	ACGT	2 cut(s)	347, 438
TaqI	TCGA	4 cut(s)	360, 615, 756, 798
TasI	AATT	3 cut(s)	118, 291, 583
TatI	WGTACW	2 cut(s)	97, 784
TauI	GCSGC	1 cut(s)	168
TfiI	GAWTC	2 cut(s)	401, 720
Tru1I	TTAA	2 cut(s)	666, 744
Tru9I	TTAA	2 cut(s)	666, 744
TscAI	CASTG	2 cut(s)	247, 258
TseFI	GTSAC	3 cut(s)	265, 436, 842
TseI	GCWGC	6 cut(s)	49, 52, 131, 144, 147, 419
Tsp45I	GTSAC	3 cut(s)	265, 436, 842
TspDTI	ATGAA	1 cut(s)	704
TspGWI	ACGGA	3 cut(s)	495, 530, 633
TspRI	CASTG	2 cut(s)	247, 258
VpaK11BI	GGWCC	2 cut(s)	557, 590
XceI	RCATGY	2 cut(s)	43, 104
XhoI	CTCGAG	1 cut(s)	359
XmiI	GTMKAC	1 cut(s)	798
XspI	CTAG	2 cut(s)	86, 859

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.