Rorug04G0211500

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012618
Physical Location & Seq
Forward (+)
36782508 .. 36782849
342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0211500.1

Sequence Viewer

Length: 342 bp
ATGTCTGAGCTCGACGTCCAGATTCCTACCACCTTTGATCCGTTTGCCGAGGCAAATGCTGAGGACTCAGGTGTTGGAACAAAAGAGTATGTGCACATTCGTATACAGCAACGAAATGGTAGGAAAATCCTGACAACAGTGCAGGGACTGAAGAAGGAATTTAGCTGCTACAAGATTCTCAAAGACCTTAAGAAGGAATTTTGCTGCAATGGTACAGTTGTCCAGGACCCAGAACAAGGCCAGGTGATTCAACTTCAAGGTGATCAGAGGAAGAACACGTCTGCCTTCCTAGTTCAGGCTGGCATTGTGAAGAAGGATAACATCAAAATTCACGGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

113

Amino Acids

12.71

Weight (kDa)

8.45

Isoelectric Point (pI)

34.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUI1 PF01253 28 - 102 3.9e-25 Translation initiation factor SUI1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 18
AccI GTMKAC 1 cut(s) 103
AclWI GGATC 1 cut(s) 32
AcsI RAATTY 3 cut(s) 158, 197, 327
AcuI CTGAAG 1 cut(s) 170
AcyI GRCGYC 1 cut(s) 15
AfaI GTAC 1 cut(s) 214
AfiI CCNNNNNNNGG 3 cut(s) 193, 236, 295
AflII CTTAAG 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 2 cut(s) 251, 257
AjiI CACGTC 1 cut(s) 279
AjnI CCWGG 2 cut(s) 222, 240
AluBI AGCT 2 cut(s) 10, 165
AluI AGCT 2 cut(s) 10, 165
Alw21I GWGCWC 2 cut(s) 12, 96
Alw44I GTGCAC 1 cut(s) 92
AlwI GGATC 1 cut(s) 32
AlwNI CAGNNNCTG 1 cut(s) 148
AoxI GGCC 1 cut(s) 238
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 2 cut(s) 165, 204
ApoI RAATTY 3 cut(s) 158, 197, 327
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 2 cut(s) 256, 272
AvaII GGWCC 1 cut(s) 226
BaeGI GKGCMC 1 cut(s) 96
BaeI ACNNNNGTAYC 2 cut(s) 204, 237
BanII GRGCYC 1 cut(s) 12
Bbv12I GWGCWC 2 cut(s) 12, 96
BbvCI CCTCAGC 1 cut(s) 60
BbvI GCAGC 2 cut(s) 152, 191
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 2 cut(s) 224, 242
BclI TGATCA 1 cut(s) 262
BfaI CTAG 1 cut(s) 290
BfrI CTTAAG 1 cut(s) 188
BisI GCNGC 2 cut(s) 166, 205
BlsI GCNGC 2 cut(s) 167, 206
Bme1390I CCNGG 2 cut(s) 224, 242
Bme18I GGWCC 1 cut(s) 226
BmgBI CACGTC 1 cut(s) 279
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 2 cut(s) 224, 242
Bpu10I CCTNAGC 1 cut(s) 60
BsaHI GRCGYC 1 cut(s) 15
BsaJI CCNNGG 1 cut(s) 48
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 236, 295
Bse3DI GCAATG 1 cut(s) 214
BseBI CCWGG 2 cut(s) 224, 242
BseDI CCNNGG 1 cut(s) 48
BseLI CCNNNNNNNGG 3 cut(s) 193, 236, 295
BseMI GCAATG 1 cut(s) 214
BseMII CTCAG 2 cut(s) 51, 81
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 2 cut(s) 152, 191
BsgI GTGCAG 1 cut(s) 161
BshFI GGCC 1 cut(s) 240
BsiHKAI GWGCWC 2 cut(s) 12, 96
BslFI GGGAC 1 cut(s) 159
BslI CCNNNNNNNGG 3 cut(s) 193, 236, 295
BsmFI GGGAC 1 cut(s) 159
BsnI GGCC 1 cut(s) 240
Bsp1286I GDGCHC 2 cut(s) 12, 96
Bsp143I GATC 2 cut(s) 37, 262
BspANI GGCC 1 cut(s) 240
BspCNI CTCAG 2 cut(s) 52, 80
BspLI GGNNCC 1 cut(s) 228
BspPI GGATC 1 cut(s) 32
BspTI CTTAAG 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 214
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 2 cut(s) 37, 262
BssNAI GTATAC 1 cut(s) 104
BssNI GRCGYC 1 cut(s) 15
Bst1107I GTATAC 1 cut(s) 104
Bst2UI CCWGG 2 cut(s) 224, 242
Bst4CI ACNGT 3 cut(s) 139, 217, 335
BstACI GRCGYC 1 cut(s) 15
BstAFI CTTAAG 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 3 cut(s) 6, 60, 67
BstENI CCTNNNNNAGG 2 cut(s) 191, 293
BstKTI GATC 2 cut(s) 40, 265
BstMBI GATC 2 cut(s) 37, 262
BstNI CCWGG 2 cut(s) 224, 242
BstSCI CCNGG 2 cut(s) 222, 240
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 152, 191
BstZ17I GTATAC 1 cut(s) 104
BsuRI GGCC 1 cut(s) 240
BtrI CACGTC 1 cut(s) 279
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 301
CaiI CAGNNNCTG 1 cut(s) 148
Cfr13I GGNCC 1 cut(s) 226
Csp6I GTAC 1 cut(s) 213
CviJI RGCY 4 cut(s) 10, 165, 240, 299
CviKI_1 RGCY 4 cut(s) 10, 165, 240, 299
CviQI GTAC 1 cut(s) 213
DdeI CTNAG 3 cut(s) 6, 60, 67
DpnI GATC 2 cut(s) 39, 264
DpnII GATC 2 cut(s) 37, 262
Ecl136II GAGCTC 1 cut(s) 10
Eco24I GRGCYC 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 226
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 10
EcoNI CCTNNNNNAGG 2 cut(s) 191, 293
EcoO109I RGGNCCY 1 cut(s) 226
EcoRII CCWGG 2 cut(s) 222, 240
EcoT38I GRGCYC 1 cut(s) 12
FaiI YATR 2 cut(s) 90, 104
FaqI GGGAC 1 cut(s) 159
FbaI TGATCA 1 cut(s) 262
FblI GTMKAC 1 cut(s) 103
Fnu4HI GCNGC 2 cut(s) 166, 205
FriOI GRGCYC 1 cut(s) 12
Fsp4HI GCNGC 2 cut(s) 166, 205
FspBI CTAG 1 cut(s) 290
GluI GCNGC 2 cut(s) 166, 205
HaeIII GGCC 1 cut(s) 240
Hin1I GRCGYC 1 cut(s) 15
HinfI GANTC 4 cut(s) 22, 65, 175, 247
HphI GGTGA 2 cut(s) 256, 272
Hpy166II GTNNAC 2 cut(s) 94, 104
Hpy188I TCNGA 3 cut(s) 7, 267, 341
Hpy188III TCNNGA 2 cut(s) 19, 130
Hpy8I GTNNAC 2 cut(s) 94, 104
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 4 cut(s) 148, 187, 295, 307
HpyCH4III ACNGT 3 cut(s) 139, 217, 335
HpyCH4IV ACGT 2 cut(s) 15, 278
HpyCH4V TGCA 3 cut(s) 94, 142, 207
HpyF3I CTNAG 3 cut(s) 6, 60, 67
HpySE526I ACGT 2 cut(s) 15, 278
Hsp92I GRCGYC 1 cut(s) 15
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 2 cut(s) 37, 262
Lsp1109I GCAGC 2 cut(s) 152, 191
MaeI CTAG 1 cut(s) 290
MaeII ACGT 2 cut(s) 15, 278
MalI GATC 2 cut(s) 39, 264
MboI GATC 2 cut(s) 37, 262
MboII GAAGA 3 cut(s) 163, 283, 322
MhlI GDGCHC 2 cut(s) 12, 96
MluCI AATT 3 cut(s) 158, 197, 327
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 1 cut(s) 55
MnlI CCTC 3 cut(s) 43, 55, 261
MseI TTAA 1 cut(s) 189
MspCI CTTAAG 1 cut(s) 188
MspR9I CCNGG 2 cut(s) 224, 242
MvaI CCWGG 2 cut(s) 224, 242
NdeII GATC 2 cut(s) 37, 262
NlaIV GGNNCC 1 cut(s) 228
NmeAIII GCCGAG 1 cut(s) 73
PfeI GAWTC 3 cut(s) 22, 175, 247
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 2 cut(s) 167, 206
PleI GAGTC 1 cut(s) 59
PpsI GAGTC 1 cut(s) 59
PpuMI RGGWCCY 1 cut(s) 226
Psp124BI GAGCTC 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 2 cut(s) 222, 240
PspGI CCWGG 2 cut(s) 222, 240
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 1 cut(s) 226
PspPPI RGGWCCY 1 cut(s) 226
PstNI CAGNNNCTG 1 cut(s) 148
RsaI GTAC 1 cut(s) 214
RsaNI GTAC 1 cut(s) 213
SacI GAGCTC 1 cut(s) 12
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 2 cut(s) 166, 205
Sau3AI GATC 2 cut(s) 37, 262
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 2 cut(s) 224, 242
SduI GDGCHC 2 cut(s) 12, 96
SetI ASST 9 cut(s) 12, 18, 35, 73, 167, 189, 246, 262, 281
SinI GGWCC 1 cut(s) 226
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 3 cut(s) 158, 197, 327
SspMI CTAG 1 cut(s) 290
SstI GAGCTC 1 cut(s) 12
StyD4I CCNGG 2 cut(s) 222, 240
TaaI ACNGT 3 cut(s) 139, 217, 335
TaiI ACGT 2 cut(s) 18, 281
TaqI TCGA 1 cut(s) 12
TasI AATT 3 cut(s) 158, 197, 327
TfiI GAWTC 3 cut(s) 22, 175, 247
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 1 cut(s) 144
TseI GCWGC 2 cut(s) 165, 204
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 1 cut(s) 144
Vha464I CTTAAG 1 cut(s) 188
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 226
XagI CCTNNNNNAGG 2 cut(s) 191, 293
XapI RAATTY 3 cut(s) 158, 197, 327
XmiI GTMKAC 1 cut(s) 103
XspI CTAG 1 cut(s) 290
ZraI GACGTC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.