Rorug04G0265400

Glycosyltransferase family 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004922
Physical Location & Seq
Forward (+)
43770297 .. 43771032
736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0265400.1

Sequence Viewer

Length: 450 bp
ATGGAGAGATCTGGTAGAGGCGGCGCCGATTTTCGGGACTTGCTTCAGAGATCCTCAGGAGGTCCGATTTCGTTCTTGAGCTTTACGAGAAACAACTCCTTGGTCAAGCAGTTCGGCCGGAGCCAAGCCAGCGTCCGTCGGTCCATTTCAACTAGGAGCCTAACAACCTCTACAACCTCCACTCCTAAAAGCATTCCGGTTTGGCAAACAAGAAGACTGTTGAGTATTCAGGCTGGAGGCAAAGACCAGTCAGTGGTGCTCGCCACCACTAAGACCAAGAAGCAGAACAAGCCAACCAGTTTGGTTAACAAGTCTGTGATGAGGAAGGATTTTTGTCGCATGGCCAAGGCTGTTACCAACCAGGTACTGAATACTCCATTCACTGTTCTGTGGAATGAAATGAATGATAATAGCAATGATGTATGTGATGCGTTTAGTTTTCTAATGTAA

Protein Analysis

149

Amino Acids

16.57

Weight (kDa)

11.16

Isoelectric Point (pI)

48.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L28e PF01778 29 - 123 4.9e-06 Ribosomal L28e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 23
AccB7I CCANNNNNTGG 1 cut(s) 253
AciI CCGC 1 cut(s) 21
AclWI GGATC 1 cut(s) 45
AcoI YGGCCR 2 cut(s) 115, 342
AcuI CTGAAG 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 1 cut(s) 366
AfiI CCNNNNNNNGG 2 cut(s) 33, 253
AgsI TTSAA 1 cut(s) 150
AjnI CCWGG 1 cut(s) 360
AluBI AGCT 1 cut(s) 81
AluI AGCT 1 cut(s) 81
Alw21I GWGCWC 1 cut(s) 261
AlwI GGATC 1 cut(s) 45
AlwNI CAGNNNCTG 1 cut(s) 367
AoxI GGCC 2 cut(s) 115, 342
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 2 cut(s) 62, 141
AvaII GGWCC 2 cut(s) 62, 141
AxyI CCTNAGG 1 cut(s) 55
BalI TGGCCA 1 cut(s) 344
BanI GGYRCC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 220
Bbv12I GWGCWC 1 cut(s) 261
BciT130I CCWGG 1 cut(s) 362
BfaI CTAG 1 cut(s) 153
BfoI RGCGCY 1 cut(s) 27
BglII AGATCT 1 cut(s) 8
BisI GCNGC 1 cut(s) 22
BlsI GCNGC 1 cut(s) 23
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 2 cut(s) 62, 141
BmgT120I GGNCC 2 cut(s) 62, 141
BmiI GGNNCC 3 cut(s) 25, 122, 158
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 1 cut(s) 418
BpiI GAAGAC 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 97
BsaHI GRCGYC 1 cut(s) 24
BsaJI CCNNGG 2 cut(s) 99, 345
BsaWI WCCGGW 1 cut(s) 196
BsaXI ACNNNNNCTCC 2 cut(s) 166, 196
Bsc4I CCNNNNNNNGG 2 cut(s) 33, 253
Bse1I ACTGG 2 cut(s) 247, 297
Bse21I CCTNAGG 1 cut(s) 55
Bse3DI GCAATG 1 cut(s) 421
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 2 cut(s) 99, 345
BseLI CCNNNNNNNGG 2 cut(s) 33, 253
BseMI GCAATG 1 cut(s) 421
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 2 cut(s) 247, 297
BseX3I CGGCCG 1 cut(s) 115
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 2 cut(s) 117, 344
BshNI GGYRCC 1 cut(s) 23
BsiEI CGRYCG 1 cut(s) 118
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 2 cut(s) 118, 197
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 2 cut(s) 33, 253
BsmFI GGGAC 1 cut(s) 50
BsmI GAATGC 1 cut(s) 192
BsnI GGCC 2 cut(s) 117, 344
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 2 cut(s) 8, 50
BspACI CCGC 1 cut(s) 21
BspANI GGCC 2 cut(s) 117, 344
BspCNI CTCAG 1 cut(s) 68
BspLI GGNNCC 3 cut(s) 25, 122, 158
BspPI GGATC 1 cut(s) 45
BspT107I GGYRCC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 421
BsrI ACTGG 2 cut(s) 247, 297
BssECI CCNNGG 2 cut(s) 99, 345
BssMI GATC 2 cut(s) 8, 50
BssNI GRCGYC 1 cut(s) 24
BssT1I CCWWGG 2 cut(s) 99, 345
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 219, 385
BstACI GRCGYC 1 cut(s) 24
BstC8I GCNNGC 2 cut(s) 130, 261
BstDEI CTNAG 2 cut(s) 55, 270
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 2 cut(s) 11, 53
BstMBI GATC 2 cut(s) 8, 50
BstMCI CGRYCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 2 cut(s) 129, 289
BstNI CCWGG 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 360
BstV2I GAAGAC 1 cut(s) 220
BstX2I RGATCY 2 cut(s) 8, 50
BstYI RGATCY 2 cut(s) 8, 50
BstZI CGGCCG 1 cut(s) 115
Bsu36I CCTNAGG 1 cut(s) 55
BsuRI GGCC 2 cut(s) 117, 344
BtsIMutI CAGTG 2 cut(s) 258, 381
Cac8I GCNNGC 2 cut(s) 130, 261
CaiI CAGNNNCTG 1 cut(s) 367
CfoI GCGC 1 cut(s) 26
Cfr13I GGNCC 2 cut(s) 62, 141
CseI GACGC 1 cut(s) 121
CsiI ACCWGGT 1 cut(s) 360
Csp6I GTAC 1 cut(s) 365
CviAII CATG 1 cut(s) 340
CviJI RGCY 9 cut(s) 81, 117, 123, 128, 159, 233, 292, 344, 350
CviKI_1 RGCY 9 cut(s) 81, 117, 123, 128, 159, 233, 292, 344, 350
CviQI GTAC 1 cut(s) 365
DdeI CTNAG 2 cut(s) 55, 270
DinI GGCGCC 1 cut(s) 25
DpnI GATC 2 cut(s) 10, 52
DpnII GATC 2 cut(s) 8, 50
EaeI YGGCCR 2 cut(s) 115, 342
EagI CGGCCG 1 cut(s) 115
EclXI CGGCCG 1 cut(s) 115
Eco130I CCWWGG 2 cut(s) 99, 345
Eco47I GGWCC 2 cut(s) 62, 141
Eco52I CGGCCG 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 29
Eco81I CCTNAGG 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 2 cut(s) 99, 345
EgeI GGCGCC 1 cut(s) 25
EheI GGCGCC 1 cut(s) 25
ErhI CCWWGG 2 cut(s) 99, 345
FaeI CATG 1 cut(s) 343
FaiI YATR 2 cut(s) 341, 424
FaqI GGGAC 1 cut(s) 50
FatI CATG 1 cut(s) 339
Fnu4HI GCNGC 1 cut(s) 22
Fsp4HI GCNGC 1 cut(s) 22
FspBI CTAG 1 cut(s) 153
GlaI GCGC 1 cut(s) 25
GluI GCNGC 1 cut(s) 22
GsuI CTGGAG 1 cut(s) 255
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 2 cut(s) 117, 344
HapII CCGG 2 cut(s) 118, 197
HgaI GACGC 1 cut(s) 121
HhaI GCGC 1 cut(s) 26
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 1 cut(s) 343
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HpaI GTTAAC 1 cut(s) 307
HpaII CCGG 2 cut(s) 118, 197
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 2 cut(s) 48, 66
Hpy188III TCNNGA 3 cut(s) 35, 57, 76
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 1 cut(s) 141
HpyAV CCTTC 1 cut(s) 319
HpyCH4III ACNGT 2 cut(s) 219, 385
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 289
HpyF3I CTNAG 2 cut(s) 55, 270
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 1 cut(s) 343
HspAI GCGC 1 cut(s) 24
KasI GGCGCC 1 cut(s) 23
KspAI GTTAAC 1 cut(s) 307
Kzo9I GATC 2 cut(s) 8, 50
LmnI GCTCC 2 cut(s) 120, 156
LweI GCATC 1 cut(s) 418
MabI ACCWGGT 1 cut(s) 360
MaeI CTAG 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 10, 52
MboI GATC 2 cut(s) 8, 50
MboII GAAGA 1 cut(s) 225
MflI RGATCY 2 cut(s) 8, 50
MhlI GDGCHC 1 cut(s) 261
MlsI TGGCCA 1 cut(s) 344
MluNI TGGCCA 1 cut(s) 344
Mly113I GGCGCC 1 cut(s) 24
MnlI CCTC 7 cut(s) 11, 53, 64, 178, 187, 230, 315
Mox20I TGGCCA 1 cut(s) 344
MscI TGGCCA 1 cut(s) 344
MseI TTAA 1 cut(s) 306
Msp20I TGGCCA 1 cut(s) 344
MspI CCGG 2 cut(s) 118, 197
MspR9I CCNGG 1 cut(s) 362
Mva1269I GAATGC 1 cut(s) 192
MvaI CCWGG 1 cut(s) 362
MwoI GCNNNNNNNGC 2 cut(s) 129, 289
NarI GGCGCC 1 cut(s) 24
NdeII GATC 2 cut(s) 8, 50
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 3 cut(s) 25, 122, 158
PctI GAATGC 1 cut(s) 192
PflMI CCANNNNNTGG 1 cut(s) 253
PkrI GCNGC 1 cut(s) 23
PluTI GGCGCC 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 3 cut(s) 25, 122, 158
PspPI GGNCC 2 cut(s) 62, 141
PstNI CAGNNNCTG 1 cut(s) 367
PsuI RGATCY 2 cut(s) 8, 50
RsaI GTAC 1 cut(s) 366
RsaNI GTAC 1 cut(s) 365
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 1 cut(s) 22
Sau3AI GATC 2 cut(s) 8, 50
Sau96I GGNCC 2 cut(s) 62, 141
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 1 cut(s) 261
SetI ASST 5 cut(s) 64, 83, 170, 179, 366
SexAI ACCWGGT 1 cut(s) 360
SfaNI GCATC 1 cut(s) 418
SfoI GGCGCC 1 cut(s) 25
SinI GGWCC 2 cut(s) 62, 141
SmlI CTYRAG 1 cut(s) 76
SmoI CTYRAG 1 cut(s) 76
SsiI CCGC 1 cut(s) 21
SspDI GGCGCC 1 cut(s) 23
SspMI CTAG 1 cut(s) 153
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 2 cut(s) 99, 345
TaaI ACNGT 2 cut(s) 219, 385
TaqII GACCGA 1 cut(s) 129
TauI GCSGC 1 cut(s) 24
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TscAI CASTG 2 cut(s) 258, 388
TspDTI ATGAA 2 cut(s) 411, 416
TspGWI ACGGA 1 cut(s) 125
TspRI CASTG 2 cut(s) 258, 388
Van91I CCANNNNNTGG 1 cut(s) 253
VpaK11BI GGWCC 2 cut(s) 62, 141
XspI CTAG 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.