Rorug04G0277800

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007709
Physical Location & Seq
Forward (+)
45043718 .. 45044353
636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0277800.1

Sequence Viewer

Length: 636 bp
ATGGATGTGGATGTTCTTAGCTGGAATTGCAGAGGAATCTGCAATGATGCTACAACTAGGGCTCTGAAGGATCTGTGCTCTCAGATTCAAGTTGGCACCAAATCTGCACATCATATCGACGCCATGGTTGTGGGTGAATCTGGGGCTCCGTCATGGAGAGTGACGGGTTTTTACGGATATGCTCGAACTGGTGAAAGGGATAGGTCATGGCAGTTGTTGCGTGATCTTTCCGATATTGATTCACTTCCTTGGGTGGTAATAGGAGATTTCAACGAGATTCTTAACAATGGGGAAAAGGTTGCAGGCCCGATACGACCGGAGAGACAAATGCGAGGCTTCCGGAAGGCATTGGGCTATTGTGATCTTCTGGATTTAGGGTATCAGGGAGCCATGGCTACTTGGTGGAATTCAGAAACTTTGCTGCGGTTAGATCGTGTTGTGTGCACCCCGTCGTGGATTGATATCTTCAACCACGCTAGAGTCGCTCACCTGGCACCTTCAGACTCAGATCATGCTCCTATACTTCTGCGCACTAGTACAACCCCTCTGTTGGTAAGACCTAAATGTCATCACTTCAAGTTTGAAGCGTTTTGGTTACAACATCCGGATTGTGATGGGGTAGTGAAGGAGGCGTGA

Protein Analysis

211

Amino Acids

23.81

Weight (kDa)

6.22

Isoelectric Point (pI)

30.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 40 - 168 4.2e-09 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 564
Acc16I TGCGCA 1 cut(s) 530
AccB1I GGYRCC 2 cut(s) 95, 493
AccIII TCCGGA 2 cut(s) 339, 604
AciI CCGC 1 cut(s) 424
AclWI GGATC 1 cut(s) 78
AcsI RAATTY 1 cut(s) 406
AcuI CTGAAG 2 cut(s) 86, 483
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 1 cut(s) 538
AfiI CCNNNNNNNGG 2 cut(s) 453, 550
AgsI TTSAA 5 cut(s) 89, 271, 469, 577, 584
AhlI ACTAGT 1 cut(s) 533
AjnI CCWGG 1 cut(s) 489
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 2 cut(s) 80, 446
Alw26I GTCTC 1 cut(s) 316
Alw44I GTGCAC 1 cut(s) 442
AlwI GGATC 1 cut(s) 78
Aor13HI TCCGGA 2 cut(s) 339, 604
AoxI GGCC 1 cut(s) 304
ApaLI GTGCAC 1 cut(s) 442
ApeKI GCWGC 1 cut(s) 421
ApoI RAATTY 1 cut(s) 406
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 1 cut(s) 305
AsuHPI GGTGA 3 cut(s) 146, 203, 479
BaeGI GKGCMC 1 cut(s) 446
BanI GGYRCC 2 cut(s) 95, 493
BanII GRGCYC 2 cut(s) 64, 148
Bbv12I GWGCWC 2 cut(s) 80, 446
BbvI GCAGC 1 cut(s) 408
BccI CCATC 1 cut(s) 608
BciT130I CCWGG 1 cut(s) 491
BcoDI GTCTC 1 cut(s) 316
BcuI ACTAGT 1 cut(s) 533
BfaI CTAG 3 cut(s) 57, 477, 534
BisI GCNGC 1 cut(s) 422
BlsI GCNGC 1 cut(s) 423
Bme1390I CCNGG 1 cut(s) 491
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 4 cut(s) 97, 147, 388, 495
BmrFI CCNGG 1 cut(s) 491
BmsI GCATC 1 cut(s) 37
BsaBI GATNNNNATC 1 cut(s) 461
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 123, 248, 390
BsaWI WCCGGW 3 cut(s) 316, 339, 604
Bsc4I CCNNNNNNNGG 2 cut(s) 453, 550
Bse1I ACTGG 1 cut(s) 193
Bse3DI GCAATG 1 cut(s) 49
Bse8I GATNNNNATC 1 cut(s) 461
BseAI TCCGGA 2 cut(s) 339, 604
BseBI CCWGG 1 cut(s) 491
BseDI CCNNGG 3 cut(s) 123, 248, 390
BseGI GGATG 3 cut(s) 10, 16, 601
BseJI GATNNNNATC 1 cut(s) 461
BseLI CCNNNNNNNGG 2 cut(s) 453, 550
BseMI GCAATG 1 cut(s) 49
BseMII CTCAG 2 cut(s) 95, 519
BseNI ACTGG 1 cut(s) 193
BseSI GKGCMC 1 cut(s) 446
BseXI GCAGC 1 cut(s) 408
BsgI GTGCAG 1 cut(s) 90
Bsh1285I CGRYCG 1 cut(s) 317
BshFI GGCC 1 cut(s) 306
BshNI GGYRCC 2 cut(s) 95, 493
BsiEI CGRYCG 1 cut(s) 317
BsiHKAI GWGCWC 2 cut(s) 80, 446
BsiSI CCGG 3 cut(s) 317, 340, 605
BslI CCNNNNNNNGG 2 cut(s) 453, 550
BsmAI GTCTC 1 cut(s) 316
BsnI GGCC 1 cut(s) 306
Bsp1286I GDGCHC 4 cut(s) 64, 80, 148, 446
Bsp13I TCCGGA 2 cut(s) 339, 604
Bsp143I GATC 5 cut(s) 70, 223, 361, 430, 508
Bsp19I CCATGG 2 cut(s) 123, 390
BspACI CCGC 1 cut(s) 424
BspANI GGCC 1 cut(s) 306
BspCNI CTCAG 2 cut(s) 94, 518
BspEI TCCGGA 2 cut(s) 339, 604
BspLI GGNNCC 4 cut(s) 97, 147, 388, 495
BspPI GGATC 1 cut(s) 78
BspT107I GGYRCC 2 cut(s) 95, 493
BsrDI GCAATG 1 cut(s) 49
BsrI ACTGG 1 cut(s) 193
BssECI CCNNGG 3 cut(s) 123, 248, 390
BssMI GATC 5 cut(s) 70, 223, 361, 430, 508
BssNI GRCGYC 1 cut(s) 120
BssT1I CCWWGG 3 cut(s) 123, 248, 390
Bst2UI CCWGG 1 cut(s) 491
BstACI GRCGYC 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 217
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 3 cut(s) 17, 81, 505
BstDSI CCRYGG 2 cut(s) 123, 390
BstF5I GGATG 3 cut(s) 10, 16, 601
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 5 cut(s) 73, 226, 364, 433, 511
BstMAI GTCTC 1 cut(s) 316
BstMBI GATC 5 cut(s) 70, 223, 361, 430, 508
BstMCI CGRYCG 1 cut(s) 317
BstMWI GCNNNNNNNGC 4 cut(s) 27, 217, 482, 491
BstNI CCWGG 1 cut(s) 491
BstSCI CCNGG 1 cut(s) 489
BstSLI GKGCMC 1 cut(s) 446
BstV1I GCAGC 1 cut(s) 408
BstX2I RGATCY 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 130
BstYI RGATCY 1 cut(s) 70
BsuRI GGCC 1 cut(s) 306
BtgI CCRYGG 2 cut(s) 123, 390
BtsCI GGATG 3 cut(s) 10, 16, 601
Cac8I GCNNGC 1 cut(s) 304
CfoI GCGC 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 305
CseI GACGC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 537
CviAII CATG 5 cut(s) 124, 153, 207, 391, 512
CviJI RGCY 8 cut(s) 21, 62, 146, 306, 336, 354, 389, 395
CviKI_1 RGCY 8 cut(s) 21, 62, 146, 306, 336, 354, 389, 395
CviQI GTAC 1 cut(s) 537
DdeI CTNAG 3 cut(s) 17, 81, 505
DpnI GATC 5 cut(s) 72, 225, 363, 432, 510
DpnII GATC 5 cut(s) 70, 223, 361, 430, 508
DrdI GACNNNNNNGTC 1 cut(s) 564
DseDI GACNNNNNNGTC 1 cut(s) 564
Eco130I CCWWGG 3 cut(s) 123, 248, 390
Eco24I GRGCYC 2 cut(s) 64, 148
Eco32I GATATC 1 cut(s) 463
Eco57I CTGAAG 2 cut(s) 86, 483
EcoRI GAATTC 1 cut(s) 406
EcoRII CCWGG 1 cut(s) 489
EcoRV GATATC 1 cut(s) 463
EcoT14I CCWWGG 3 cut(s) 123, 248, 390
EcoT38I GRGCYC 2 cut(s) 64, 148
ErhI CCWWGG 3 cut(s) 123, 248, 390
FaeI CATG 5 cut(s) 127, 156, 210, 394, 515
FaiI YATR 8 cut(s) 114, 125, 154, 180, 208, 392, 513, 521
FatI CATG 5 cut(s) 123, 152, 206, 390, 511
Fnu4HI GCNGC 1 cut(s) 422
FokI GGATG 3 cut(s) 17, 23, 588
FriOI GRGCYC 2 cut(s) 64, 148
Fsp4HI GCNGC 1 cut(s) 422
FspBI CTAG 3 cut(s) 57, 477, 534
FspI TGCGCA 1 cut(s) 530
GlaI GCGC 1 cut(s) 530
GluI GCNGC 1 cut(s) 422
HaeIII GGCC 1 cut(s) 306
HapII CCGG 3 cut(s) 317, 340, 605
HgaI GACGC 1 cut(s) 128
HhaI GCGC 1 cut(s) 531
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 5 cut(s) 127, 156, 210, 394, 515
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HinfI GANTC 7 cut(s) 36, 85, 137, 239, 277, 480, 503
HpaII CCGG 3 cut(s) 317, 340, 605
HphI GGTGA 3 cut(s) 146, 203, 479
Hpy166II GTNNAC 1 cut(s) 444
Hpy188I TCNGA 6 cut(s) 66, 84, 232, 412, 502, 508
Hpy188III TCNNGA 3 cut(s) 340, 368, 605
Hpy8I GTNNAC 1 cut(s) 444
Hpy99I CGWCG 2 cut(s) 122, 454
HpyAV CCTTC 4 cut(s) 61, 337, 507, 619
HpyCH4V TGCA 5 cut(s) 30, 42, 107, 302, 444
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 217, 482, 491
HpyF3I CTNAG 3 cut(s) 17, 81, 505
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 5 cut(s) 127, 156, 210, 394, 515
HspAI GCGC 1 cut(s) 529
Kpn2I TCCGGA 2 cut(s) 339, 604
Kzo9I GATC 5 cut(s) 70, 223, 361, 430, 508
LmnI GCTCC 3 cut(s) 151, 386, 520
Lsp1109I GCAGC 1 cut(s) 408
LweI GCATC 1 cut(s) 37
MaeI CTAG 3 cut(s) 57, 477, 534
MaeIII GTNAC 2 cut(s) 160, 594
MalI GATC 5 cut(s) 72, 225, 363, 432, 510
MboI GATC 5 cut(s) 70, 223, 361, 430, 508
MboII GAAGA 2 cut(s) 356, 457
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 4 cut(s) 64, 80, 148, 446
MluCI AATT 2 cut(s) 25, 406
MlyI GAGTC 2 cut(s) 489, 497
MnlI CCTC 4 cut(s) 26, 326, 555, 622
MroI TCCGGA 2 cut(s) 339, 604
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 128
MspI CCGG 3 cut(s) 317, 340, 605
MspR9I CCNGG 1 cut(s) 491
MvaI CCWGG 1 cut(s) 491
MwoI GCNNNNNNNGC 4 cut(s) 27, 217, 482, 491
NcoI CCATGG 2 cut(s) 123, 390
NdeII GATC 5 cut(s) 70, 223, 361, 430, 508
NlaIII CATG 5 cut(s) 127, 156, 210, 394, 515
NlaIV GGNNCC 4 cut(s) 97, 147, 388, 495
NmuCI GTSAC 1 cut(s) 160
NsbI TGCGCA 1 cut(s) 530
PfeI GAWTC 5 cut(s) 36, 85, 137, 239, 277
PkrI GCNGC 1 cut(s) 423
PleI GAGTC 2 cut(s) 488, 497
PpsI GAGTC 2 cut(s) 488, 497
Psp6I CCWGG 1 cut(s) 489
PspGI CCWGG 1 cut(s) 489
PspN4I GGNNCC 4 cut(s) 97, 147, 388, 495
PspPI GGNCC 1 cut(s) 305
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 1 cut(s) 538
RsaNI GTAC 1 cut(s) 537
RseI CAYNNNNRTG 1 cut(s) 128
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 1 cut(s) 422
Sau3AI GATC 5 cut(s) 70, 223, 361, 430, 508
Sau96I GGNCC 1 cut(s) 305
SchI GAGTC 2 cut(s) 489, 497
ScrFI CCNGG 1 cut(s) 491
SduI GDGCHC 4 cut(s) 64, 80, 148, 446
SetI ASST 6 cut(s) 23, 206, 300, 492, 499, 562
SfaNI GCATC 1 cut(s) 37
SmiMI CAYNNNNRTG 1 cut(s) 128
SpeI ACTAGT 1 cut(s) 533
Sse9I AATT 2 cut(s) 25, 406
SsiI CCGC 1 cut(s) 424
SspMI CTAG 3 cut(s) 57, 477, 534
StyD4I CCNGG 1 cut(s) 489
StyI CCWWGG 3 cut(s) 123, 248, 390
TaqI TCGA 2 cut(s) 117, 184
TasI AATT 2 cut(s) 25, 406
TatI WGTACW 1 cut(s) 536
TfiI GAWTC 5 cut(s) 36, 85, 137, 239, 277
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 1 cut(s) 421
Tsp45I GTSAC 1 cut(s) 160
TspGWI ACGGA 2 cut(s) 138, 189
VneI GTGCAC 1 cut(s) 442
XapI RAATTY 1 cut(s) 406
XspI CTAG 3 cut(s) 57, 477, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.