Rh4DG336300

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007709
Physical Location & Seq
Forward (+)
55973626 .. 55974990
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG336300.1

Sequence Viewer

Length: 1365 bp
ATGGCAGCCAAGGTCTTCTTCCTCTTTCTCTCTCTACTATTTCTCCTTATCTCTCTAAACCACGCAACTTCAAACCCAGATTCCGAGCCTCTCTTAGCGTTCAAGGCCACGTCGGACTCTTCCAATGCTCTCGCCACGTGGAACTCATCCTCCGACCCGTGCACCACCTGGGTCGGAGTCTCCTGCACCCGGAGCCGGGTCTCTCGGCTCGTTCTCGAGAACCTCGGCCTTAGCGGCTCGTTCGTGGCGCTGACTGGGTTGACTCAGCTCCGAGTTCTCAGCCTGAAGCGGAACCGCCTCTCCGGCCCCATTCCCGACCTCTCTAACCTCACTTCCCTCAAGCTCCTCTTCCTCTCCTACAACGACTTCTCCGGCGACTTTCCCGCTTCCGTAACGTCCCTTTTCCGGCTTTACCGCCTCGATCTCGCTTACAACAACTTGTCCGGTGAAATTCCCGAGACCGTCAACCATTTGACTCATCTCCTCACTCTCCGCCTCGAAGCGAACCGGTTCTCCGGCTCGATTTCGGGCCTCAACATCCCTAACCTCCAGGAGTTCAACGTCTCGGCGAACCGTTTGTCCGGCGAAATTCCCAAGTCGTTGTCTATTTTCTCCGAGTCTGCATTTGCGCAAAACCCGGGTCTGTGCGGGTCTCCGGTTCAGAACTGTAAAACAGAAACCGACCCGACTCGACCCGGAGGAGCTATTGCGTCTCCTGTAATACCTGCAAGAAACCCAACCGTAGTGGCGTCCTCGCCGACTTCATTGCCATCAAGCTCTACGTCCACCAAATCGGAAAAGTCAACGTCCCACCACAACGGAGGTTCCAGAATTAGTCCGGCGGCTCTGATCGCCATAATAGTCGGCGACGTGTTGGTCCTCTTTTTGGTCTCGCTGCTGCTCTACTGCTACTTCTGGCGGAACTTCGCGGCCAAAATGCGGCAGGGCAAGAGCAGCTCGAAGCTTCTGGAGACAGAGAAGATAGTCTACTCGTCGAGCCCATACTCGGCCCAGCCCACTTTCGAGAGGGGACAGATGGTGTTTTTCGAAGGCGTGAAGCGTTTCGAGCTGGAGGACTTGCTGAGAGCCTCGGCGGAGATGTTGGGGAAAGGTGGGTTCGGGACTGCGTACAAGGCGGTTTTGGACGACGGCAATGTTGTGGCGGTGAAGAGGCTCAAGGATGCTCAAATTGGTGGGAAGAATCAGTTTGAGCAGCACATGGCGGTGCTGGGCAAGCTTAGCCATCTCAACATTGTGAGCTTGAGGGCTTATTATTTCGCTAGGGAGGAGAAGTTGCTGGTCTACGATTACATGCCAAATGGAAGCTTGTTTTGGCTTCTTCATGGTAAGTTGCTTCATCTTTAA

Protein Analysis

454

Amino Acids

49.54

Weight (kDa)

9.23

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 63 4.7e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 83 - 177 5.6e-07 Leucine-rich repeat region
Pkinase PF00069 366 - 448 8e-11 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 366 - 448 3.7e-10 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 875
Acc16I TGCGCA 1 cut(s) 630
Acc36I ACCTGC 1 cut(s) 733
AccI GTMKAC 2 cut(s) 987, 1302
AccII CGCG 1 cut(s) 929
AcoI YGGCCR 1 cut(s) 930
AcsI RAATTY 2 cut(s) 450, 588
AcuI CTGAAG 1 cut(s) 305
AcvI CACGTG 1 cut(s) 138
AcyI GRCGYC 1 cut(s) 749
AfaI GTAC 1 cut(s) 1130
AfiI CCNNNNNNNGG 7 cut(s) 189, 195, 196, 405, 886, 939, 1006
AflIII ACRYGT 1 cut(s) 870
AgeI ACCGGT 1 cut(s) 507
AgsI TTSAA 3 cut(s) 72, 103, 559
AjiI CACGTC 2 cut(s) 111, 871
AjnI CCWGG 2 cut(s) 167, 549
AjuI GAANNNNNNNTTGG 3 cut(s) 34, 1315, 1347
AloI GAACNNNNNNTCC 8 cut(s) 134, 166, 284, 316, 497, 529, 563, 595
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 8 cut(s) 184, 205, 452, 568, 657, 717, 895, 965
Alw44I GTGCAC 1 cut(s) 160
Ama87I CYCGRG 3 cut(s) 215, 455, 637
AoxI GGCC 6 cut(s) 105, 226, 304, 529, 930, 1008
ApaLI GTGCAC 1 cut(s) 160
ApeKI GCWGC 5 cut(s) 5, 895, 898, 954, 1213
ApoI RAATTY 2 cut(s) 450, 588
ArsI GACNNNNNNTTYG 4 cut(s) 563, 595, 625, 657
AsiGI ACCGGT 1 cut(s) 507
Asp700I GAANNNNTTC 2 cut(s) 509, 1061
AspLEI GCGC 2 cut(s) 250, 631
AspS9I GGNCC 4 cut(s) 305, 529, 877, 1009
AsuC2I CCSGG 5 cut(s) 190, 197, 638, 639, 696
AsuHPI GGTGA 2 cut(s) 458, 1177
AsuII TTCGAA 1 cut(s) 1047
AvaI CYCGRG 3 cut(s) 215, 455, 637
AvaII GGWCC 1 cut(s) 877
BaeGI GKGCMC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 1001
BarI GAAGNNNNNNTAC 2 cut(s) 971, 1003
BbrPI CACGTG 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 7
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 5 cut(s) 17, 882, 885, 966, 1225
BccI CCATC 3 cut(s) 778, 1030, 1251
BceAI ACGGC 1 cut(s) 1165
BcgI CGANNNNNNTGC 2 cut(s) 748, 782
BciT130I CCWGG 2 cut(s) 169, 551
BcnI CCSGG 5 cut(s) 190, 197, 638, 639, 696
BcoDI GTCTC 8 cut(s) 184, 205, 452, 568, 657, 717, 895, 965
BfaI CTAG 1 cut(s) 1281
BfoI RGCGCY 1 cut(s) 251
BfuAI ACCTGC 1 cut(s) 733
BglI GCCNNNNNGGC 2 cut(s) 234, 303
BisI GCNGC 9 cut(s) 6, 235, 843, 896, 899, 930, 941, 955, 1214
BlpI GCTNAGC 1 cut(s) 1238
BlsI GCNGC 9 cut(s) 7, 236, 844, 897, 900, 931, 942, 956, 1215
Bme1390I CCNGG 7 cut(s) 169, 190, 197, 551, 638, 639, 696
Bme18I GGWCC 1 cut(s) 877
BmeT110I CYCGRG 3 cut(s) 215, 455, 637
BmgBI CACGTC 2 cut(s) 111, 871
BmgT120I GGNCC 4 cut(s) 305, 529, 877, 1009
BmiI GGNNCC 4 cut(s) 194, 293, 307, 826
BmrFI CCNGG 7 cut(s) 169, 190, 197, 551, 638, 639, 696
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 1 cut(s) 1171
BmuI ACTGGG 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 7
BpmI CTGGAG 3 cut(s) 533, 989, 1091
Bpu10I CCTNAGC 1 cut(s) 230
Bpu1102I GCTNAGC 1 cut(s) 1238
Bpu14I TTCGAA 1 cut(s) 1047
BpuEI CTTGAG 3 cut(s) 323, 1160, 1282
BpuMI CCSGG 5 cut(s) 190, 197, 638, 639, 696
BsaAI YACGTR 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 749
BsaI GGTCTC 4 cut(s) 205, 452, 657, 895
BsaJI CCNNGG 5 cut(s) 9, 168, 223, 637, 1089
BsaWI WCCGGW 3 cut(s) 443, 507, 655
Bsc4I CCNNNNNNNGG 7 cut(s) 189, 195, 196, 405, 886, 939, 1006
Bse118I RCCGGY 1 cut(s) 507
Bse1I ACTGG 1 cut(s) 259
Bse3DI GCAATG 2 cut(s) 764, 1159
BseBI CCWGG 2 cut(s) 169, 551
BseDI CCNNGG 5 cut(s) 9, 168, 223, 637, 1089
BseGI GGATG 3 cut(s) 146, 537, 1186
BseLI CCNNNNNNNGG 7 cut(s) 189, 195, 196, 405, 886, 939, 1006
BseMI GCAATG 2 cut(s) 764, 1159
BseMII CTCAG 3 cut(s) 278, 292, 1073
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 4 cut(s) 335, 473, 714, 1301
BseSI GKGCMC 1 cut(s) 164
BseXI GCAGC 5 cut(s) 17, 882, 885, 966, 1225
BseYI CCCAGC 2 cut(s) 1011, 1228
BsgI GTGCAG 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 929
BshFI GGCC 6 cut(s) 107, 228, 306, 531, 932, 1010
BshTI ACCGGT 1 cut(s) 507
BsiHKAI GWGCWC 1 cut(s) 164
BsiHKCI CYCGRG 3 cut(s) 215, 455, 637
BslFI GGGAC 4 cut(s) 382, 793, 1044, 1135
BslI CCNNNNNNNGG 7 cut(s) 189, 195, 196, 405, 886, 939, 1006
BsmAI GTCTC 8 cut(s) 184, 205, 452, 568, 657, 717, 895, 965
BsmBI CGTCTC 2 cut(s) 568, 717
BsmFI GGGAC 4 cut(s) 382, 793, 1044, 1135
BsnI GGCC 6 cut(s) 107, 228, 306, 531, 932, 1010
Bso31I GGTCTC 4 cut(s) 205, 452, 657, 895
BsoBI CYCGRG 3 cut(s) 215, 455, 637
Bsp119I TTCGAA 1 cut(s) 1047
Bsp1286I GDGCHC 2 cut(s) 164, 1001
Bsp143I GATC 2 cut(s) 421, 849
Bsp1720I GCTNAGC 1 cut(s) 1238
BspANI GGCC 6 cut(s) 107, 228, 306, 531, 932, 1010
BspCNI CTCAG 3 cut(s) 277, 291, 1074
BspFNI CGCG 1 cut(s) 929
BspLI GGNNCC 4 cut(s) 194, 293, 307, 826
BspMI ACCTGC 1 cut(s) 733
BspT104I TTCGAA 1 cut(s) 1047
BspTNI GGTCTC 4 cut(s) 205, 452, 657, 895
BsrDI GCAATG 2 cut(s) 764, 1159
BsrFI RCCGGY 1 cut(s) 507
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 507
BssECI CCNNGG 5 cut(s) 9, 168, 223, 637, 1089
BssMI GATC 2 cut(s) 421, 849
BssNI GRCGYC 1 cut(s) 749
BssT1I CCWWGG 1 cut(s) 9
Bst2UI CCWGG 2 cut(s) 169, 551
Bst4CI ACNGT 4 cut(s) 463, 575, 668, 742
Bst6I CTCTTC 3 cut(s) 124, 353, 1163
BstACI GRCGYC 1 cut(s) 749
BstBAI YACGTR 1 cut(s) 138
BstBI TTCGAA 1 cut(s) 1047
BstC8I GCNNGC 1 cut(s) 1235
BstDEI CTNAG 6 cut(s) 94, 230, 264, 278, 1082, 1238
BstF5I GGATG 3 cut(s) 146, 537, 1186
BstFNI CGCG 1 cut(s) 929
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 2 cut(s) 250, 631
BstKTI GATC 2 cut(s) 424, 852
BstMAI GTCTC 8 cut(s) 184, 205, 452, 568, 657, 717, 895, 965
BstMBI GATC 2 cut(s) 421, 849
BstNI CCWGG 2 cut(s) 169, 551
BstNSI RCATGY 1 cut(s) 1315
BstSCI CCNGG 7 cut(s) 167, 188, 195, 549, 636, 637, 694
BstSLI GKGCMC 1 cut(s) 164
BstUI CGCG 1 cut(s) 929
BstV1I GCAGC 5 cut(s) 17, 882, 885, 966, 1225
BstV2I GAAGAC 1 cut(s) 7
BsuRI GGCC 6 cut(s) 107, 228, 306, 531, 932, 1010
BtrI CACGTC 2 cut(s) 111, 871
BtsCI GGATG 3 cut(s) 146, 537, 1186
BveI ACCTGC 1 cut(s) 733
Cac8I GCNNGC 1 cut(s) 1235
CfoI GCGC 2 cut(s) 250, 631
Cfr10I RCCGGY 1 cut(s) 507
Cfr13I GGNCC 4 cut(s) 305, 529, 877, 1009
Cfr9I CCCGGG 1 cut(s) 637
CseI GACGC 2 cut(s) 699, 738
Csp6I GTAC 1 cut(s) 1129
CspAI ACCGGT 1 cut(s) 507
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviAII CATG 3 cut(s) 1219, 1312, 1343
CviQI GTAC 1 cut(s) 1129
DdeI CTNAG 6 cut(s) 94, 230, 264, 278, 1082, 1238
DpnI GATC 2 cut(s) 423, 851
DpnII GATC 2 cut(s) 421, 849
DrdI GACNNNNNNGTC 1 cut(s) 875
DseDI GACNNNNNNGTC 1 cut(s) 875
EaeI YGGCCR 1 cut(s) 930
Eam1104I CTCTTC 3 cut(s) 124, 353, 1163
EarI CTCTTC 3 cut(s) 124, 353, 1163
EciI GGCGGA 3 cut(s) 482, 934, 1109
Eco130I CCWWGG 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 1001
Eco31I GGTCTC 4 cut(s) 205, 452, 657, 895
Eco47I GGWCC 1 cut(s) 877
Eco57I CTGAAG 1 cut(s) 305
Eco72I CACGTG 1 cut(s) 138
Eco88I CYCGRG 3 cut(s) 215, 455, 637
EcoRII CCWGG 2 cut(s) 167, 549
EcoT14I CCWWGG 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 1001
ErhI CCWWGG 1 cut(s) 9
Esp3I CGTCTC 2 cut(s) 568, 717
FaeI CATG 3 cut(s) 1222, 1315, 1346
FaiI YATR 5 cut(s) 857, 1003, 1220, 1313, 1344
FalI AAGNNNNNCTT 3 cut(s) 34, 332, 364
FaqI GGGAC 4 cut(s) 382, 793, 1044, 1135
FatI CATG 3 cut(s) 1218, 1311, 1342
FauI CCCGC 2 cut(s) 391, 641
FblI GTMKAC 2 cut(s) 987, 1302
Fnu4HI GCNGC 9 cut(s) 6, 235, 843, 896, 899, 930, 941, 955, 1214
FokI GGATG 3 cut(s) 133, 524, 1193
FriOI GRGCYC 1 cut(s) 1001
Fsp4HI GCNGC 9 cut(s) 6, 235, 843, 896, 899, 930, 941, 955, 1214
FspBI CTAG 1 cut(s) 1281
FspI TGCGCA 1 cut(s) 630
GlaI GCGC 2 cut(s) 249, 630
GluI GCNGC 9 cut(s) 6, 235, 843, 896, 899, 930, 941, 955, 1214
GsaI CCCAGC 2 cut(s) 1015, 1232
GsuI CTGGAG 3 cut(s) 533, 989, 1091
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 6 cut(s) 107, 228, 306, 531, 932, 1010
HgaI GACGC 2 cut(s) 699, 738
HhaI GCGC 2 cut(s) 250, 631
Hin1I GRCGYC 1 cut(s) 749
Hin1II CATG 3 cut(s) 1222, 1315, 1346
Hin6I GCGC 2 cut(s) 248, 629
HinP1I GCGC 2 cut(s) 248, 629
HincII GTYRAC 3 cut(s) 261, 466, 804
HindII GTYRAC 3 cut(s) 261, 466, 804
HindIII AAGCTT 3 cut(s) 962, 1235, 1324
HinfI GANTC 8 cut(s) 80, 116, 177, 262, 475, 617, 688, 1201
HphI GGTGA 2 cut(s) 458, 1177
Hpy166II GTNNAC 7 cut(s) 162, 261, 466, 786, 804, 988, 1303
Hpy188I TCNGA 9 cut(s) 85, 115, 154, 176, 272, 616, 663, 796, 849
Hpy188III TCNNGA 8 cut(s) 215, 217, 314, 455, 828, 968, 1024, 1120
Hpy8I GTNNAC 7 cut(s) 162, 261, 466, 786, 804, 988, 1303
Hpy99I CGWCG 4 cut(s) 115, 872, 997, 1151
HpyAV CCTTC 1 cut(s) 1043
HpyCH4III ACNGT 4 cut(s) 463, 575, 668, 742
HpyCH4IV ACGT 7 cut(s) 110, 137, 395, 561, 782, 806, 870
HpyCH4V TGCA 4 cut(s) 162, 186, 623, 728
HpyF3I CTNAG 6 cut(s) 94, 230, 264, 278, 1082, 1238
HpySE526I ACGT 7 cut(s) 110, 137, 395, 561, 782, 806, 870
Hsp92I GRCGYC 1 cut(s) 749
Hsp92II CATG 3 cut(s) 1222, 1315, 1346
HspAI GCGC 2 cut(s) 248, 629
Kzo9I GATC 2 cut(s) 421, 849
LmnI GCTCC 4 cut(s) 192, 273, 348, 701
Lsp1109I GCAGC 5 cut(s) 17, 882, 885, 966, 1225
LweI GCATC 1 cut(s) 1171
MaeI CTAG 1 cut(s) 1281
MaeII ACGT 7 cut(s) 110, 137, 395, 561, 782, 806, 870
MaeIII GTNAC 1 cut(s) 391
MalI GATC 2 cut(s) 423, 851
MboI GATC 2 cut(s) 421, 849
MboII GAAGA 8 cut(s) 7, 10, 111, 340, 991, 1180, 1210, 1331
MhlI GDGCHC 2 cut(s) 164, 1001
MluCI AATT 4 cut(s) 450, 588, 831, 1188
MlyI GAGTC 6 cut(s) 110, 186, 256, 469, 626, 682
MmeI TCCRAC 3 cut(s) 93, 154, 177
MroXI GAANNNNTTC 2 cut(s) 509, 1061
MseI TTAA 1 cut(s) 1363
MslI CAYNNNNRTG 1 cut(s) 1223
MspR9I CCNGG 7 cut(s) 169, 190, 197, 551, 638, 639, 696
MvaI CCWGG 2 cut(s) 169, 551
MvnI CGCG 1 cut(s) 929
NciI CCSGG 5 cut(s) 190, 197, 638, 639, 696
NdeII GATC 2 cut(s) 421, 849
NlaIII CATG 3 cut(s) 1222, 1315, 1346
NlaIV GGNNCC 4 cut(s) 194, 293, 307, 826
NmeAIII GCCGAG 5 cut(s) 184, 204, 545, 986, 1070
NsbI TGCGCA 1 cut(s) 630
NspI RCATGY 1 cut(s) 1315
NspV TTCGAA 1 cut(s) 1047
PaeR7I CTCGAG 1 cut(s) 215
PdmI GAANNNNTTC 2 cut(s) 509, 1061
PfeI GAWTC 2 cut(s) 80, 1201
PfoI TCCNGGA 1 cut(s) 549
PinAI ACCGGT 1 cut(s) 507
PkrI GCNGC 9 cut(s) 7, 236, 844, 897, 900, 931, 942, 956, 1215
PleI GAGTC 6 cut(s) 110, 185, 256, 469, 625, 682
PmaCI CACGTG 1 cut(s) 138
PmlI CACGTG 1 cut(s) 138
PpsI GAGTC 6 cut(s) 110, 185, 256, 469, 625, 682
Ppu21I YACGTR 1 cut(s) 138
Psp6I CCWGG 2 cut(s) 167, 549
PspCI CACGTG 1 cut(s) 138
PspFI CCCAGC 2 cut(s) 1011, 1228
PspGI CCWGG 2 cut(s) 167, 549
PspN4I GGNNCC 4 cut(s) 194, 293, 307, 826
PspPI GGNCC 4 cut(s) 305, 529, 877, 1009
RsaI GTAC 1 cut(s) 1130
RsaNI GTAC 1 cut(s) 1129
RseI CAYNNNNRTG 1 cut(s) 1223
SaqAI TTAA 1 cut(s) 1363
SatI GCNGC 9 cut(s) 6, 235, 843, 896, 899, 930, 941, 955, 1214
Sau3AI GATC 2 cut(s) 421, 849
Sau96I GGNCC 4 cut(s) 305, 529, 877, 1009
SchI GAGTC 6 cut(s) 110, 186, 256, 469, 626, 682
ScrFI CCNGG 7 cut(s) 169, 190, 197, 551, 638, 639, 696
SduI GDGCHC 2 cut(s) 164, 1001
SfaNI GCATC 1 cut(s) 1171
Sfr274I CTCGAG 1 cut(s) 215
SfuI TTCGAA 1 cut(s) 1047
SinI GGWCC 1 cut(s) 877
SlaI CTCGAG 1 cut(s) 215
SmaI CCCGGG 1 cut(s) 639
SmiMI CAYNNNNRTG 1 cut(s) 1223
SmlI CTYRAG 4 cut(s) 215, 338, 1175, 1261
SmoI CTYRAG 4 cut(s) 215, 338, 1175, 1261
Sse9I AATT 4 cut(s) 450, 588, 831, 1188
SspMI CTAG 1 cut(s) 1281
StyD4I CCNGG 7 cut(s) 167, 188, 195, 549, 636, 637, 694
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 4 cut(s) 463, 575, 668, 742
TaiI ACGT 7 cut(s) 113, 140, 398, 564, 785, 809, 873
TasI AATT 4 cut(s) 450, 588, 831, 1188
TauI GCSGC 4 cut(s) 237, 845, 932, 943
TfiI GAWTC 2 cut(s) 80, 1201
Tru1I TTAA 1 cut(s) 1363
Tru9I TTAA 1 cut(s) 1363
TseI GCWGC 5 cut(s) 5, 895, 898, 954, 1213
TspDTI ATGAA 3 cut(s) 753, 1331, 1346
TspGWI ACGGA 2 cut(s) 379, 834
TspMI CCCGGG 1 cut(s) 637
VneI GTGCAC 1 cut(s) 160
VpaK11BI GGWCC 1 cut(s) 877
XapI RAATTY 2 cut(s) 450, 588
XceI RCATGY 1 cut(s) 1315
XhoI CTCGAG 1 cut(s) 215
XmaI CCCGGG 1 cut(s) 637
XmiI GTMKAC 2 cut(s) 987, 1302
XmnI GAANNNNTTC 2 cut(s) 509, 1061
XspI CTAG 1 cut(s) 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.