Rh4BG342600

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0007709
Physical Location & Seq
Forward (+)
50578946 .. 50581914
2969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG342600.1

Sequence Viewer

Length: 2118 bp
ATGCCCCTCAACTTCCCATTAATTACGTTCATACCCTATCCCCTCTATTTTTCACTACCTCACTCCTCACTCCCTCTACTCTCATTTGCAGAGACAGCAATGGCAACCAAGGCCTTCTTCTTCTTTCTCTCTCTACTATTTCTCATTATCTCTCTAATCCACGCAACTTCAAACCCAGATTCCCAGCCTCTCTTAGCGTTCAAGGCCACGTCGGACGCTTCCAATGCTCTCGCCACGTGGAACTCATCCTCCGACCCGTGCACCACCTGGGTCGGAGTCTCCTGCACCCGGAGCCGGGTCTCGCGGCTCGTTCTCGAGAACCTCGGCCTTAGCGGCTCGTTGGCGTCGCTGACTGGGTTGACGCAGCTCCGAGTTCTCAGCCTGAAGCGGAACCGCCTCTCCGGCCCCATTCCCGACCTCTCTAACCTCAATTCCCTCAAGCTCCTCTTCCTCTCCTACAACGACTTCTCCGGCGACTTTCCCGCTTCCGTAACGTCCCTTTTCCGGCTTTACCGCCTCGATCTCGCTTACAACAACTTGTCCGGTGAAATTCCCGAGACGGTCAACCATTTGACTCATCTCCTCACTCTCCGCCTCGAAGCGAACCGATTTTCCGGCTCGATTTCCGGCCTCAACATCCCTAACCTCCAGGAGTTCAACGTCTCGGCGAACCGTTTGTCCGGCGAAATTCCCAAGTCGTTGTCTATTTTCTCCGAGTCTGCATTTGCGCAAAACCCGGGTCTGTGCGGGTCTCCGGTTCAGAACTGTAAAACAGAAACCGACCCTACTCGACCCGGAGGAGCTATTGCGTCTCCTGTAATACCCGCCGGAAACCCAACCGTAGTGGCGTCCTCGCCGACTTCATTGCCATCAAGCTCTACGCCCACCAAATCGGAAAAGTCGACGTCCCACCACAACGGAGGTTCCAAAATTAGTCCGGCGGCTCTGATCGCCATAATAGTCGGCGACGTGTTGGTCCTCTTTTTGGTCTCGCTGCTGCTCTACTGCTACTTCTGGCGGAACTTCGCGGCCAAAATGCGGCAGGGCAAGAGCAGCTCGAAGCTTCTGGAGACAGAGAAGATAGTCTACTCGTCGAGCCCATACTCGGCCCAGCCCTCTTTCGAGAGGGGACAGATGGTGTTTTTCGAAGGCGTGAAGCGTTTCGAGCTGGAGGACTTGCTGAGAGCCTCGGCGGAGATGTTGGGGAAAGGTGGGTTCGGGACGGCGTACAAGGCGGTTTTGGACGACGGCAATGTTGTGGCGGTGAAGAGGCTCAAGGATGCTCAAATTGGTGGGAAGAATCAGTTTGAGCAGCACATGGCGGTGCTGGGCAAGCTTAACCATCTCAACATTGTGAGCTTGCGGGCTTATTATTTCGCTAGGGAGGAGAAGTTGCTGGTCTACGATTACATGCCAAATGGAAGCTTGTTTTGGCTTCTTCATGGTAACAGAGGACCTGGAAGAACCCCACTGGACTGGACCACCAGACTGAAAATTGCAGCCGGAGCTGCACGAGGGCTAGGTTTCATTCACAGCTCCTGCGCTCCCCTTAAGCTCACCCACGGCAACATCAAATCCACCAACATTCTCTTGGACAAGACCGGCAATGCTCGTGTCTCCGACTTCGGACTTTCGGCTTTTTCGCCTTCCACTTCCGCCGCGTCGGCGCCGCGGTCGTGCGGGTACCGCGCTCCGGAGTTGCTGGAGGGTCGGAAAGTAACGCAGAAGTCGGACGTGTATGCTTTCGGTGTTCTTTTGCTGGAGATATTGACCGGGAAATGCCCGTCCGCGGTGGTGGATGGCGGGTCCGGCGGCGGATACGGCGGGTTGGTGGACCTGCCGAGGTGGGTCCAGTCTGTGGTGAGAGAGGAGTGGACTGCCGAGGTGTTTGACTTGGAGTTGATGAGGTACAAGGACATTGAGGAGGAGATGGTGGGGCTTTTGCAGGTTGCAATGGCTTGCACAACGGCAGTGCCTGATCAACGGCCGAGGATGAGCCAGGTGGTGAAGATGATCGACGAGATTCGCGGCGTGGCGGGGTCACCGCCTCATGAGGCGTGTGATTCCATGACCGAGTCGCCGGCTTTGTCCGAGGACACTTGCGGAGCAAGTCAGTGA

Protein Analysis

705

Amino Acids

76.25

Weight (kDa)

8.35

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 58 - 96 8.4e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 114 - 209 1.4e-06 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 399 - 671 1.7e-36 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 399 - 598 6.4e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 974
AatII GACGTC 1 cut(s) 908
Acc16I TGCGCA 1 cut(s) 729
Acc36I ACCTGC 2 cut(s) 1845, 1936
Acc65I GGTACC 1 cut(s) 1683
AccB1I GGYRCC 2 cut(s) 1666, 1683
AccB7I CCANNNNNTGG 1 cut(s) 1858
AccI GTMKAC 3 cut(s) 902, 1086, 1401
AccII CGCG 7 cut(s) 304, 1028, 1661, 1672, 1689, 1790, 2028
AccIII TCCGGA 1 cut(s) 1693
AcoI YGGCCR 2 cut(s) 1029, 1985
AcsI RAATTY 2 cut(s) 549, 687
AcuI CTGAAG 1 cut(s) 404
AcvI CACGTG 1 cut(s) 237
AcyI GRCGYC 4 cut(s) 344, 848, 905, 1667
AfaI GTAC 3 cut(s) 1229, 1685, 1910
AflII CTTAAG 1 cut(s) 1550
AflIII ACRYGT 2 cut(s) 969, 1734
AgsI TTSAA 3 cut(s) 171, 202, 658
AjiI CACGTC 3 cut(s) 210, 970, 1735
AjnI CCWGG 4 cut(s) 266, 648, 1456, 1998
AjuI GAANNNNNNNTTGG 4 cut(s) 101, 133, 1414, 1446
AloI GAACNNNNNNTCC 8 cut(s) 233, 265, 383, 415, 596, 628, 662, 694
Alw21I GWGCWC 1 cut(s) 263
Alw44I GTGCAC 1 cut(s) 259
AlwNI CAGNNNCTG 2 cut(s) 1539, 1976
Ama87I CYCGRG 3 cut(s) 314, 554, 736
Aor13HI TCCGGA 1 cut(s) 1693
AoxI GGCC 8 cut(s) 111, 204, 325, 403, 628, 1029, 1107, 1985
ApaLI GTGCAC 1 cut(s) 259
ApeKI GCWGC 7 cut(s) 364, 994, 997, 1053, 1312, 1499, 1508
ApoI RAATTY 2 cut(s) 549, 687
ArsI GACNNNNNNTTYG 4 cut(s) 662, 694, 724, 756
AseI ATTAAT 1 cut(s) 20
Asp700I GAANNNNTTC 1 cut(s) 1160
Asp718I GGTACC 1 cut(s) 1683
AspLEI GCGC 4 cut(s) 730, 1544, 1669, 1691
AspS9I GGNCC 8 cut(s) 404, 976, 1108, 1454, 1479, 1806, 1834, 1849
AsuC2I CCSGG 6 cut(s) 289, 296, 737, 738, 795, 1774
AsuHPI GGTGA 6 cut(s) 557, 1276, 1549, 1873, 2017, 2034
AsuII TTCGAA 1 cut(s) 1146
AvaI CYCGRG 3 cut(s) 314, 554, 736
AvaII GGWCC 6 cut(s) 976, 1454, 1479, 1806, 1834, 1849
BaeGI GKGCMC 1 cut(s) 263
BanI GGYRCC 2 cut(s) 1666, 1683
BanII GRGCYC 1 cut(s) 1100
BarI GAAGNNNNNNTAC 2 cut(s) 1070, 1102
BauI CACGAG 2 cut(s) 1512, 1611
BbrPI CACGTG 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 263
BbvI GCAGC 7 cut(s) 376, 981, 984, 1065, 1324, 1495, 1511
BccI CCATC 5 cut(s) 877, 1129, 1350, 1793, 1924
BceAI ACGGC 6 cut(s) 1239, 1264, 1579, 1837, 1983, 2000
BcgI CGANNNNNNTGC 2 cut(s) 847, 881
BciT130I CCWGG 4 cut(s) 268, 650, 1458, 2000
BciVI GTATCC 1 cut(s) 1811
BclI TGATCA 1 cut(s) 1978
BcnI CCSGG 6 cut(s) 289, 296, 737, 738, 795, 1774
BfaI CTAG 2 cut(s) 1380, 1520
BfoI RGCGCY 1 cut(s) 1670
BfrI CTTAAG 1 cut(s) 1550
BfuAI ACCTGC 2 cut(s) 1845, 1936
BfuI GTATCC 1 cut(s) 1811
BglI GCCNNNNNGGC 3 cut(s) 333, 402, 1664
Bme18I GGWCC 6 cut(s) 976, 1454, 1479, 1806, 1834, 1849
BmeT110I CYCGRG 3 cut(s) 314, 554, 736
BmgBI CACGTC 3 cut(s) 210, 970, 1735
BmgT120I GGNCC 8 cut(s) 404, 976, 1108, 1454, 1479, 1806, 1834, 1849
BmiI GGNNCC 8 cut(s) 293, 392, 406, 925, 1668, 1685, 1807, 1850
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 1 cut(s) 1270
BmuI ACTGGG 1 cut(s) 363
BpmI CTGGAG 5 cut(s) 632, 1088, 1190, 1724, 1781
Bpu10I CCTNAGC 1 cut(s) 329
Bpu14I TTCGAA 1 cut(s) 1146
BpuEI CTTGAG 2 cut(s) 422, 1259
BpuMI CCSGG 6 cut(s) 289, 296, 737, 738, 795, 1774
BsaAI YACGTR 1 cut(s) 237
BsaHI GRCGYC 4 cut(s) 344, 848, 905, 1667
BsaI GGTCTC 3 cut(s) 304, 756, 994
BsaWI WCCGGW 3 cut(s) 542, 754, 1693
BsaXI ACNNNNNCTCC 8 cut(s) 233, 263, 383, 413, 452, 482, 1376, 1406
Bse118I RCCGGY 2 cut(s) 1601, 2080
Bse1I ACTGG 4 cut(s) 358, 1476, 1481, 1852
Bse3DI GCAATG 5 cut(s) 105, 863, 1258, 1612, 1959
BseAI TCCGGA 1 cut(s) 1693
BseBI CCWGG 4 cut(s) 268, 650, 1458, 2000
BseGI GGATG 5 cut(s) 245, 636, 1285, 1804, 1998
BseMI GCAATG 5 cut(s) 105, 863, 1258, 1612, 1959
BseMII CTCAG 2 cut(s) 391, 1172
BseNI ACTGG 4 cut(s) 358, 1476, 1481, 1852
BseRI GAGGAG 8 cut(s) 55, 434, 572, 813, 1400, 1883, 1937, 1940
BseSI GKGCMC 1 cut(s) 263
BseX3I CGGCCG 1 cut(s) 1985
BseXI GCAGC 7 cut(s) 376, 981, 984, 1065, 1324, 1495, 1511
BseYI CCCAGC 3 cut(s) 183, 1110, 1327
BsgI GTGCAG 2 cut(s) 268, 1494
Bsh1236I CGCG 7 cut(s) 304, 1028, 1661, 1672, 1689, 1790, 2028
Bsh1285I CGRYCG 2 cut(s) 1676, 1988
BshFI GGCC 8 cut(s) 113, 206, 327, 405, 630, 1031, 1109, 1987
BshNI GGYRCC 2 cut(s) 1666, 1683
BsiEI CGRYCG 2 cut(s) 1676, 1988
BsiHKAI GWGCWC 1 cut(s) 263
BsiHKCI CYCGRG 3 cut(s) 314, 554, 736
BslFI GGGAC 4 cut(s) 481, 892, 1143, 1234
BsmBI CGTCTC 3 cut(s) 551, 667, 816
BsmFI GGGAC 4 cut(s) 481, 892, 1143, 1234
BsnI GGCC 8 cut(s) 113, 206, 327, 405, 630, 1031, 1109, 1987
Bso31I GGTCTC 3 cut(s) 304, 756, 994
BsoBI CYCGRG 3 cut(s) 314, 554, 736
Bsp119I TTCGAA 1 cut(s) 1146
Bsp1286I GDGCHC 2 cut(s) 263, 1100
Bsp13I TCCGGA 1 cut(s) 1693
Bsp143I GATC 4 cut(s) 520, 948, 1978, 2013
BspANI GGCC 8 cut(s) 113, 206, 327, 405, 630, 1031, 1109, 1987
BspCNI CTCAG 2 cut(s) 390, 1173
BspEI TCCGGA 1 cut(s) 1693
BspFNI CGCG 7 cut(s) 304, 1028, 1661, 1672, 1689, 1790, 2028
BspHI TCATGA 1 cut(s) 2050
BspLI GGNNCC 8 cut(s) 293, 392, 406, 925, 1668, 1685, 1807, 1850
BspMI ACCTGC 2 cut(s) 1845, 1936
BspT104I TTCGAA 1 cut(s) 1146
BspT107I GGYRCC 2 cut(s) 1666, 1683
BspTI CTTAAG 1 cut(s) 1550
BspTNI GGTCTC 3 cut(s) 304, 756, 994
BsrDI GCAATG 5 cut(s) 105, 863, 1258, 1612, 1959
BsrFI RCCGGY 2 cut(s) 1601, 2080
BsrI ACTGG 4 cut(s) 358, 1476, 1481, 1852
BssAI RCCGGY 2 cut(s) 1601, 2080
BssMI GATC 4 cut(s) 520, 948, 1978, 2013
BssNI GRCGYC 4 cut(s) 344, 848, 905, 1667
BssSI CACGAG 2 cut(s) 1512, 1611
BssT1I CCWWGG 1 cut(s) 108
Bst2BI CACGAG 2 cut(s) 1512, 1611
Bst2UI CCWGG 4 cut(s) 268, 650, 1458, 2000
Bst4CI ACNGT 4 cut(s) 562, 674, 767, 841
Bst6I CTCTTC 2 cut(s) 452, 1262
BstACI GRCGYC 4 cut(s) 344, 848, 905, 1667
BstAFI CTTAAG 1 cut(s) 1550
BstBAI YACGTR 1 cut(s) 237
BstBI TTCGAA 1 cut(s) 1146
BstC8I GCNNGC 5 cut(s) 1334, 1361, 1365, 1960, 2082
BstDEI CTNAG 4 cut(s) 193, 329, 377, 1181
BstDSI CCRYGG 3 cut(s) 1561, 1670, 1788
BstEII GGTNACC 1 cut(s) 2040
BstF5I GGATG 5 cut(s) 245, 636, 1285, 1804, 1998
BstFNI CGCG 7 cut(s) 304, 1028, 1661, 1672, 1689, 1790, 2028
BstH2I RGCGCY 1 cut(s) 1670
BstHHI GCGC 4 cut(s) 730, 1544, 1669, 1691
BstKTI GATC 4 cut(s) 523, 951, 1981, 2016
BstMBI GATC 4 cut(s) 520, 948, 1978, 2013
BstMCI CGRYCG 2 cut(s) 1676, 1988
BstNI CCWGG 4 cut(s) 268, 650, 1458, 2000
BstNSI RCATGY 1 cut(s) 1414
BstPI GGTNACC 1 cut(s) 2040
BstSLI GKGCMC 1 cut(s) 263
BstUI CGCG 7 cut(s) 304, 1028, 1661, 1672, 1689, 1790, 2028
BstV1I GCAGC 7 cut(s) 376, 981, 984, 1065, 1324, 1495, 1511
BstXI CCANNNNNNTGG 1 cut(s) 1476
BstZI CGGCCG 1 cut(s) 1985
BsuI GTATCC 1 cut(s) 1811
BsuRI GGCC 8 cut(s) 113, 206, 327, 405, 630, 1031, 1109, 1987
BtgI CCRYGG 3 cut(s) 1561, 1670, 1788
BtrI CACGTC 3 cut(s) 210, 970, 1735
BtsCI GGATG 5 cut(s) 245, 636, 1285, 1804, 1998
BtsI GCAGTG 1 cut(s) 1977
BtsIMutI CAGTG 2 cut(s) 1469, 1977
BveI ACCTGC 2 cut(s) 1845, 1936
Cac8I GCNNGC 5 cut(s) 1334, 1361, 1365, 1960, 2082
CaiI CAGNNNCTG 2 cut(s) 1539, 1976
CciI TCATGA 1 cut(s) 2050
CfoI GCGC 4 cut(s) 730, 1544, 1669, 1691
Cfr10I RCCGGY 2 cut(s) 1601, 2080
Cfr13I GGNCC 8 cut(s) 404, 976, 1108, 1454, 1479, 1806, 1834, 1849
Cfr42I CCGCGG 2 cut(s) 1673, 1791
Cfr9I CCCGGG 1 cut(s) 736
CseI GACGC 6 cut(s) 224, 333, 370, 798, 837, 1650
Csp6I GTAC 3 cut(s) 1228, 1684, 1909
CspCI CAANNNNNGTGG 2 cut(s) 825, 860
CviAII CATG 5 cut(s) 1318, 1411, 1442, 2051, 2068
CviQI GTAC 3 cut(s) 1228, 1684, 1909
DdeI CTNAG 4 cut(s) 193, 329, 377, 1181
DinI GGCGCC 1 cut(s) 1668
DpnI GATC 4 cut(s) 522, 950, 1980, 2015
DpnII GATC 4 cut(s) 520, 948, 1978, 2013
DrdI GACNNNNNNGTC 1 cut(s) 974
DseDI GACNNNNNNGTC 1 cut(s) 974
EaeI YGGCCR 2 cut(s) 1029, 1985
EagI CGGCCG 1 cut(s) 1985
Eam1104I CTCTTC 2 cut(s) 452, 1262
EarI CTCTTC 2 cut(s) 452, 1262
EciI GGCGGA 5 cut(s) 581, 1033, 1208, 1645, 1830
EclXI CGGCCG 1 cut(s) 1985
Eco130I CCWWGG 1 cut(s) 108
Eco147I AGGCCT 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 1100
Eco31I GGTCTC 3 cut(s) 304, 756, 994
Eco47I GGWCC 6 cut(s) 976, 1454, 1479, 1806, 1834, 1849
Eco52I CGGCCG 1 cut(s) 1985
Eco57I CTGAAG 1 cut(s) 404
Eco72I CACGTG 1 cut(s) 237
Eco88I CYCGRG 3 cut(s) 314, 554, 736
Eco91I GGTNACC 1 cut(s) 2040
EcoO109I RGGNCCY 1 cut(s) 1454
EcoO65I GGTNACC 1 cut(s) 2040
EcoRII CCWGG 4 cut(s) 266, 648, 1456, 1998
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 1 cut(s) 1100
EgeI GGCGCC 1 cut(s) 1668
EheI GGCGCC 1 cut(s) 1668
ErhI CCWWGG 1 cut(s) 108
Esp3I CGTCTC 3 cut(s) 551, 667, 816
FaeI CATG 5 cut(s) 1321, 1414, 1445, 2054, 2071
FaiI YATR 9 cut(s) 32, 956, 1102, 1319, 1412, 1443, 1740, 2052, 2069
FalI AAGNNNNNCTT 4 cut(s) 101, 133, 431, 463
FaqI GGGAC 4 cut(s) 481, 892, 1143, 1234
FatI CATG 5 cut(s) 1317, 1410, 1441, 2050, 2067
FauI CCCGC 8 cut(s) 490, 740, 832, 1356, 1673, 1796, 1817, 2029
FbaI TGATCA 1 cut(s) 1978
FblI GTMKAC 3 cut(s) 902, 1086, 1401
FokI GGATG 5 cut(s) 232, 623, 1292, 1811, 2005
FriOI GRGCYC 1 cut(s) 1100
FspBI CTAG 2 cut(s) 1380, 1520
FspI TGCGCA 1 cut(s) 729
GlaI GCGC 4 cut(s) 729, 1543, 1668, 1690
GsaI CCCAGC 3 cut(s) 187, 1114, 1331
GsuI CTGGAG 5 cut(s) 632, 1088, 1190, 1724, 1781
HaeII RGCGCY 1 cut(s) 1670
HaeIII GGCC 8 cut(s) 113, 206, 327, 405, 630, 1031, 1109, 1987
HgaI GACGC 6 cut(s) 224, 333, 370, 798, 837, 1650
HhaI GCGC 4 cut(s) 730, 1544, 1669, 1691
Hin1I GRCGYC 4 cut(s) 344, 848, 905, 1667
Hin1II CATG 5 cut(s) 1321, 1414, 1445, 2054, 2071
Hin6I GCGC 4 cut(s) 728, 1542, 1667, 1689
HinP1I GCGC 4 cut(s) 728, 1542, 1667, 1689
HincII GTYRAC 3 cut(s) 360, 565, 903
HindII GTYRAC 3 cut(s) 360, 565, 903
HindIII AAGCTT 3 cut(s) 1061, 1334, 1423
HinfI GANTC 8 cut(s) 179, 276, 574, 716, 1300, 2023, 2063, 2075
HphI GGTGA 6 cut(s) 557, 1276, 1549, 1873, 2017, 2034
Hpy166II GTNNAC 8 cut(s) 261, 360, 565, 903, 1087, 1402, 1834, 1875
Hpy188III TCNNGA 9 cut(s) 314, 316, 413, 554, 1067, 1123, 1219, 1694, 2051
Hpy8I GTNNAC 8 cut(s) 261, 360, 565, 903, 1087, 1402, 1834, 1875
Hpy99I CGWCG 8 cut(s) 214, 349, 907, 971, 1096, 1250, 1666, 2021
HpyAV CCTTC 3 cut(s) 124, 1142, 1656
HpyCH4III ACNGT 4 cut(s) 562, 674, 767, 841
HpyCH4IV ACGT 8 cut(s) 26, 209, 236, 494, 660, 905, 969, 1734
HpyCH4V TGCA 9 cut(s) 89, 261, 285, 722, 1499, 1511, 1945, 1952, 1962
HpyF3I CTNAG 4 cut(s) 193, 329, 377, 1181
HpySE526I ACGT 8 cut(s) 26, 209, 236, 494, 660, 905, 969, 1734
Hsp92I GRCGYC 4 cut(s) 344, 848, 905, 1667
Hsp92II CATG 5 cut(s) 1321, 1414, 1445, 2054, 2071
HspAI GCGC 4 cut(s) 728, 1542, 1667, 1689
KasI GGCGCC 1 cut(s) 1666
Kpn2I TCCGGA 1 cut(s) 1693
KpnI GGTACC 1 cut(s) 1687
KroI GCCGGC 1 cut(s) 2080
KroNI GCCGGC 1 cut(s) 2082
Ksp22I TGATCA 1 cut(s) 1978
KspI CCGCGG 2 cut(s) 1673, 1791
Kzo9I GATC 4 cut(s) 520, 948, 1978, 2013
LmnI GCTCC 9 cut(s) 291, 372, 447, 800, 1505, 1541, 1549, 1696, 2105
Lsp1109I GCAGC 7 cut(s) 376, 981, 984, 1065, 1324, 1495, 1511
LweI GCATC 1 cut(s) 1270
MaeI CTAG 2 cut(s) 1380, 1520
MaeII ACGT 8 cut(s) 26, 209, 236, 494, 660, 905, 969, 1734
MaeIII GTNAC 4 cut(s) 490, 1445, 1717, 2040
MalI GATC 4 cut(s) 522, 950, 1980, 2015
MboI GATC 4 cut(s) 520, 948, 1978, 2013
MboII GAAGA 9 cut(s) 109, 112, 439, 1090, 1279, 1309, 1430, 1473, 2020
MhlI GDGCHC 2 cut(s) 263, 1100
MluCI AATT 7 cut(s) 21, 430, 549, 687, 930, 1287, 1494
Mly113I GGCGCC 1 cut(s) 1667
MlyI GAGTC 4 cut(s) 285, 568, 725, 2084
MmeI TCCRAC 6 cut(s) 192, 253, 276, 1644, 1691, 1710
MroI TCCGGA 1 cut(s) 1693
MroNI GCCGGC 1 cut(s) 2080
MroXI GAANNNNTTC 1 cut(s) 1160
MseI TTAA 3 cut(s) 20, 1338, 1551
MslI CAYNNNNRTG 1 cut(s) 1322
MspA1I CMGCKG 2 cut(s) 1672, 1790
MspCI CTTAAG 1 cut(s) 1550
MvaI CCWGG 4 cut(s) 268, 650, 1458, 2000
MvnI CGCG 7 cut(s) 304, 1028, 1661, 1672, 1689, 1790, 2028
NaeI GCCGGC 1 cut(s) 2082
NarI GGCGCC 1 cut(s) 1667
NciI CCSGG 6 cut(s) 289, 296, 737, 738, 795, 1774
NdeII GATC 4 cut(s) 520, 948, 1978, 2013
NgoMIV GCCGGC 1 cut(s) 2080
NlaIII CATG 5 cut(s) 1321, 1414, 1445, 2054, 2071
NlaIV GGNNCC 8 cut(s) 293, 392, 406, 925, 1668, 1685, 1807, 1850
NmeAIII GCCGAG 7 cut(s) 303, 644, 1085, 1169, 1866, 1906, 2013
NmuCI GTSAC 1 cut(s) 2040
NsbI TGCGCA 1 cut(s) 729
NspI RCATGY 1 cut(s) 1414
NspV TTCGAA 1 cut(s) 1146
PaeR7I CTCGAG 1 cut(s) 314
PagI TCATGA 1 cut(s) 2050
PceI AGGCCT 1 cut(s) 113
PcsI WCGNNNNNNNCGW 1 cut(s) 899
PdiI GCCGGC 1 cut(s) 2082
PdmI GAANNNNTTC 1 cut(s) 1160
PfeI GAWTC 4 cut(s) 179, 1300, 2023, 2063
PflFI GACNNNGTC 1 cut(s) 2074
PflMI CCANNNNNTGG 1 cut(s) 1858
PfoI TCCNGGA 1 cut(s) 648
PleI GAGTC 4 cut(s) 284, 568, 724, 2083
PluTI GGCGCC 1 cut(s) 1670
PmaCI CACGTG 1 cut(s) 237
PmlI CACGTG 1 cut(s) 237
PpsI GAGTC 4 cut(s) 284, 568, 724, 2083
Ppu21I YACGTR 1 cut(s) 237
PpuMI RGGWCCY 1 cut(s) 1454
PshBI ATTAAT 1 cut(s) 20
Psp5II RGGWCCY 1 cut(s) 1454
Psp6I CCWGG 4 cut(s) 266, 648, 1456, 1998
PspCI CACGTG 1 cut(s) 237
PspEI GGTNACC 1 cut(s) 2040
PspFI CCCAGC 3 cut(s) 183, 1110, 1327
PspGI CCWGG 4 cut(s) 266, 648, 1456, 1998
PspN4I GGNNCC 8 cut(s) 293, 392, 406, 925, 1668, 1685, 1807, 1850
PspPI GGNCC 8 cut(s) 404, 976, 1108, 1454, 1479, 1806, 1834, 1849
PspPPI RGGWCCY 1 cut(s) 1454
PstNI CAGNNNCTG 2 cut(s) 1539, 1976
PsyI GACNNNGTC 1 cut(s) 2074
RsaI GTAC 3 cut(s) 1229, 1685, 1910
RsaNI GTAC 3 cut(s) 1228, 1684, 1909
RseI CAYNNNNRTG 1 cut(s) 1322
SacII CCGCGG 2 cut(s) 1673, 1791
SalI GTCGAC 1 cut(s) 901
SaqAI TTAA 3 cut(s) 20, 1338, 1551
Sau3AI GATC 4 cut(s) 520, 948, 1978, 2013
Sau96I GGNCC 8 cut(s) 404, 976, 1108, 1454, 1479, 1806, 1834, 1849
SchI GAGTC 4 cut(s) 285, 568, 725, 2084
SduI GDGCHC 2 cut(s) 263, 1100
SfaNI GCATC 1 cut(s) 1270
SfoI GGCGCC 1 cut(s) 1668
Sfr274I CTCGAG 1 cut(s) 314
Sfr303I CCGCGG 2 cut(s) 1673, 1791
SfuI TTCGAA 1 cut(s) 1146
SgrBI CCGCGG 2 cut(s) 1673, 1791
SinI GGWCC 6 cut(s) 976, 1454, 1479, 1806, 1834, 1849
SlaI CTCGAG 1 cut(s) 314
SmaI CCCGGG 1 cut(s) 738
SmiMI CAYNNNNRTG 1 cut(s) 1322
SmlI CTYRAG 4 cut(s) 314, 437, 1274, 1550
SmoI CTYRAG 4 cut(s) 314, 437, 1274, 1550
Sse9I AATT 7 cut(s) 21, 430, 549, 687, 930, 1287, 1494
SseBI AGGCCT 1 cut(s) 113
SspDI GGCGCC 1 cut(s) 1666
SspMI CTAG 2 cut(s) 1380, 1520
StuI AGGCCT 1 cut(s) 113
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 4 cut(s) 562, 674, 767, 841
TaiI ACGT 8 cut(s) 29, 212, 239, 497, 663, 908, 972, 1737
TaqII GACCGA 1 cut(s) 2087
TasI AATT 7 cut(s) 21, 430, 549, 687, 930, 1287, 1494
TauI GCSGC 9 cut(s) 307, 336, 944, 1031, 1042, 1661, 1672, 1815, 2031
TfiI GAWTC 4 cut(s) 179, 1300, 2023, 2063
Tru1I TTAA 3 cut(s) 20, 1338, 1551
Tru9I TTAA 3 cut(s) 20, 1338, 1551
TscAI CASTG 2 cut(s) 1476, 1977
TseFI GTSAC 1 cut(s) 2040
TseI GCWGC 7 cut(s) 364, 994, 997, 1053, 1312, 1499, 1508
Tsp45I GTSAC 1 cut(s) 2040
TspDTI ATGAA 4 cut(s) 19, 852, 1430, 1516
TspGWI ACGGA 2 cut(s) 478, 933
TspMI CCCGGG 1 cut(s) 736
TspRI CASTG 2 cut(s) 1476, 1977
Tth111I GACNNNGTC 1 cut(s) 2074
Van91I CCANNNNNTGG 1 cut(s) 1858
Vha464I CTTAAG 1 cut(s) 1550
VneI GTGCAC 1 cut(s) 259
VpaK11BI GGWCC 6 cut(s) 976, 1454, 1479, 1806, 1834, 1849
VspI ATTAAT 1 cut(s) 20
XapI RAATTY 2 cut(s) 549, 687
XceI RCATGY 1 cut(s) 1414
XcmI CCANNNNNNNNNTGG 1 cut(s) 1588
XhoI CTCGAG 1 cut(s) 314
XmaI CCCGGG 1 cut(s) 736
XmiI GTMKAC 3 cut(s) 902, 1086, 1401
XmnI GAANNNNTTC 1 cut(s) 1160
XspI CTAG 2 cut(s) 1380, 1520
ZraI GACGTC 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.