Rw4G029010

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0007709
Physical Location & Seq
Forward (+)
55710837 .. 55713703
2867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029010.1

Sequence Viewer

Length: 2019 bp
ATGGCAGCCAAGGCCTTCTTCTTCTTTCTCTCTCTACTATTTCTCATTATCTCTCTAATCCACGCAACTTCAAACCCAGATTCCGAGCCTCTCTTAGCGTTCAAGGCCACGTCGGACGCTTCCAATGCTCTCGCCACGTGGAACTCATCCTCCGACCCGTGCACCACCTGGGTCGGAGTCTCCTGCACCCGGAGCCGGGTCTCGCGGCTCGTTCTCGAGAACCTCGGCCTTAGCGGCTCGTTCGCGGCGCTGACTGGGTTGACTCAGCTCCGAGTTCTCAGCCTGAAGCGGAACCGCCTCTCCGGCCCCATTCCCGACCTCTCTAACCTCACTTCCCTCAAGCTCCTCTTCCTCTCCTACAACGACTTCTCCGGCGACTTTCCCGCTTCCGTAACGTCCCTTTTCCGGCTTTACCGCCTCGATCTCGCTTACAACAACTTGTCCGGTGAAATTCCCGAGACCGTCAACCATTTGACTCATCTCCTCACTCTCCGCCTCGAAGCGAACCGGTTCTCCGGCTCGATTTCCGGCCTCAACATCCCTAACCTCCAGGAGTTCAACGTCTCGGCGAACCGTTTGTCCGGCGAAATTCCCAAGTCGTTGTCTATTTTCTCCGAGTCTGCATTTGCGCAAAACCCGGGTCTGTGCGGGTCTCCGGTTCAGAACTGTAACACAGAAACCGACCCGACTCGACCCGGAGGAGCTATTGCGTCTCCTGTAATACCTGCAAGAAACCCAACCGTAGTGGCGTCCTCGCCGACTTCATTGCCATCAAGCTCTACGTCCACCAAATCGGAAAAGTCGACGTCCCACCACAACGGAGGTTCCAGAATTAGTCCGGCGGCTCTGATCGCCATAATAGTCGGCGACGTGTTGGTCCTCTTTTTGGTCTCGCTGCTGCTCTACTGCTACTTCTGGCGGAACTTCGCGGCCAAAATGCGGCAGGGCAAGAGCAGCTCGAAGCTTCTGGAGACAGAGAAGATAGTCTACTCGTCGAGCCCATACTCGGCCCAGCCCACTTTCGAGAGGGGACAGATGGTGTTTTTCGAAGGCGTGAAGCGTTTCGAGCTGGAGGACTTGCTGAGAGCCTCGGCGGAGATGTTGGGGAAAGGTGGGTTCGGGACGGCGTACAAGGCGGTTTTGGACGACGGCAATGTTGTGGCGGTGAAGAGGCTCAAGGATGCTCAAATTGGTGGGAAGAATCAGTTCGAGCAGCACATGGCGGTGCTGGGCAAGCTTAGCCATCTCAACATTGTGAGCTTGAGGGCTTATTATTTTGCTAGGGAGGAGAAGTTGCTGGTCTACGATTACATGCCAAATGGAAGCTTGTTTTGGCTTCTTCACGGTAACAGAGGACCTGGTAGAACCCCACTGGACTGGACCACCAGACTGAAAATTGCAGCCGGAGCTGCACGAGGGCTAGGTTTCATTCACAGCTCCTGCGCTCCCCTTAAGCTCACCCATGGCAACATCAAATCCACCAACATTCTCTTGGACAAGACCGGCAATGCTCGTGTCTCCGACTTCGGACTTTCGGCCTTTTCGCCTTCCACTTCCGCCGCGTCGGCTCCGCGGTCGTGCGGGTACCGCGCTCCCGAGTTGCTGGAGGGTCGGAAAGTAACGCAGAAGTCGGACGTGTATGCTTTCGGTGTTCTTTTGCTGGAGATATTGACCGGGAAATGCCCGTCAGCTGTCGTGGATGGGGGCTCCGGTGGCGGATACGGTGGGTTGGTGGACCTGCCGAGGTGGGTCCAGTCTGTGGTGAGAGAGGAGTGGACTGCCGAGGTGTTCGACTTGGAGTTGATGAGGTACAAGGACATAGAGGAGGAGATGGTGGGGCTTTTGCAGGTTGCAATGGCTTGCACAGCGGCAGTGCCTGATCAACGGCCGAGGATGAGCCAGGTGGTGAAGATGATCGACGAGATTCGCGGCGTGGCGGGGTCGCCGCCTCATGAGGCGTTTGATTCCATGACGGAGTCGCCGGCTTTGTCCGAGGACACTTGCGGAGCAAGTCAGTGA

Protein Analysis

672

Amino Acids

72.61

Weight (kDa)

8.4

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 63 1.4e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 83 - 176 1.2e-06 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 366 - 638 1.2e-36 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 366 - 565 8.9e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 875
AatII GACGTC 1 cut(s) 809
Acc16I TGCGCA 1 cut(s) 630
Acc36I ACCTGC 3 cut(s) 733, 1746, 1837
Acc65I GGTACC 1 cut(s) 1584
AccB1I GGYRCC 1 cut(s) 1584
AccB7I CCANNNNNTGG 1 cut(s) 1759
AccI GTMKAC 3 cut(s) 803, 987, 1302
AccII CGCG 7 cut(s) 205, 245, 929, 1562, 1573, 1590, 1929
AcoI YGGCCR 2 cut(s) 930, 1886
AcsI RAATTY 2 cut(s) 450, 588
AcuI CTGAAG 1 cut(s) 305
AcvI CACGTG 1 cut(s) 138
AcyI GRCGYC 2 cut(s) 749, 806
AfaI GTAC 3 cut(s) 1130, 1586, 1811
AfiI CCNNNNNNNGG 8 cut(s) 189, 195, 196, 405, 886, 939, 1006, 1759
AflII CTTAAG 1 cut(s) 1451
AflIII ACRYGT 2 cut(s) 870, 1635
AgeI ACCGGT 1 cut(s) 507
AgsI TTSAA 3 cut(s) 72, 103, 559
AjiI CACGTC 3 cut(s) 111, 871, 1636
AjnI CCWGG 4 cut(s) 167, 549, 1357, 1899
AjuI GAANNNNNNNTTGG 3 cut(s) 34, 1315, 1347
AloI GAACNNNNNNTCC 8 cut(s) 134, 166, 284, 316, 497, 529, 563, 595
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 9 cut(s) 184, 205, 452, 568, 657, 717, 895, 965, 1522
Alw44I GTGCAC 1 cut(s) 160
AlwNI CAGNNNCTG 2 cut(s) 1440, 1877
Ama87I CYCGRG 4 cut(s) 215, 455, 637, 1595
AoxI GGCC 9 cut(s) 12, 105, 226, 304, 529, 930, 1008, 1536, 1886
ApaLI GTGCAC 1 cut(s) 160
ApeKI GCWGC 7 cut(s) 5, 895, 898, 954, 1213, 1400, 1409
ApoI RAATTY 2 cut(s) 450, 588
ArsI GACNNNNNNTTYG 4 cut(s) 563, 595, 625, 657
AsiGI ACCGGT 1 cut(s) 507
Asp700I GAANNNNTTC 3 cut(s) 509, 1061, 1205
Asp718I GGTACC 1 cut(s) 1584
AspLEI GCGC 4 cut(s) 250, 631, 1445, 1592
AspS9I GGNCC 7 cut(s) 305, 877, 1009, 1355, 1380, 1735, 1750
AsuC2I CCSGG 6 cut(s) 190, 197, 638, 639, 696, 1675
AsuHPI GGTGA 5 cut(s) 458, 1177, 1450, 1774, 1918
AsuII TTCGAA 1 cut(s) 1047
AvaI CYCGRG 4 cut(s) 215, 455, 637, 1595
AvaII GGWCC 5 cut(s) 877, 1355, 1380, 1735, 1750
BaeGI GKGCMC 1 cut(s) 164
BanI GGYRCC 1 cut(s) 1584
BanII GRGCYC 2 cut(s) 1001, 1709
BarI GAAGNNNNNNTAC 2 cut(s) 971, 1003
BauI CACGAG 2 cut(s) 1413, 1512
BbrPI CACGTG 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 7 cut(s) 17, 882, 885, 966, 1225, 1396, 1412
BccI CCATC 5 cut(s) 778, 1030, 1251, 1694, 1825
BceAI ACGGC 3 cut(s) 1140, 1165, 1901
BcgI CGANNNNNNTGC 2 cut(s) 748, 782
BciT130I CCWGG 4 cut(s) 169, 551, 1359, 1901
BciVI GTATCC 1 cut(s) 1712
BclI TGATCA 1 cut(s) 1879
BcnI CCSGG 6 cut(s) 190, 197, 638, 639, 696, 1675
BcoDI GTCTC 9 cut(s) 184, 205, 452, 568, 657, 717, 895, 965, 1522
BfaI CTAG 2 cut(s) 1281, 1421
BfoI RGCGCY 1 cut(s) 251
BfrI CTTAAG 1 cut(s) 1451
BfuAI ACCTGC 3 cut(s) 733, 1746, 1837
BfuI GTATCC 1 cut(s) 1712
BglI GCCNNNNNGGC 3 cut(s) 234, 303, 1565
BlpI GCTNAGC 1 cut(s) 1238
Bme18I GGWCC 5 cut(s) 877, 1355, 1380, 1735, 1750
BmeT110I CYCGRG 4 cut(s) 215, 455, 637, 1595
BmgBI CACGTC 3 cut(s) 111, 871, 1636
BmgT120I GGNCC 7 cut(s) 305, 877, 1009, 1355, 1380, 1735, 1750
BmiI GGNNCC 8 cut(s) 194, 293, 307, 826, 1569, 1586, 1708, 1751
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 1 cut(s) 1171
BmuI ACTGGG 1 cut(s) 264
BpmI CTGGAG 5 cut(s) 533, 989, 1091, 1625, 1682
Bpu10I CCTNAGC 1 cut(s) 230
Bpu1102I GCTNAGC 1 cut(s) 1238
Bpu14I TTCGAA 1 cut(s) 1047
BpuEI CTTGAG 3 cut(s) 323, 1160, 1282
BpuMI CCSGG 6 cut(s) 190, 197, 638, 639, 696, 1675
BsaAI YACGTR 1 cut(s) 138
BsaHI GRCGYC 2 cut(s) 749, 806
BsaI GGTCTC 4 cut(s) 205, 452, 657, 895
BsaWI WCCGGW 4 cut(s) 443, 507, 655, 1709
Bsc4I CCNNNNNNNGG 8 cut(s) 189, 195, 196, 405, 886, 939, 1006, 1759
Bse118I RCCGGY 3 cut(s) 507, 1502, 1981
Bse1I ACTGG 4 cut(s) 259, 1377, 1382, 1753
Bse3DI GCAATG 4 cut(s) 764, 1159, 1513, 1860
BseBI CCWGG 4 cut(s) 169, 551, 1359, 1901
BseGI GGATG 5 cut(s) 146, 537, 1186, 1705, 1899
BseLI CCNNNNNNNGG 8 cut(s) 189, 195, 196, 405, 886, 939, 1006, 1759
BseMI GCAATG 4 cut(s) 764, 1159, 1513, 1860
BseMII CTCAG 3 cut(s) 278, 292, 1073
BseNI ACTGG 4 cut(s) 259, 1377, 1382, 1753
BseRI GAGGAG 7 cut(s) 335, 473, 714, 1301, 1784, 1838, 1841
BseSI GKGCMC 1 cut(s) 164
BseX3I CGGCCG 1 cut(s) 1886
BseXI GCAGC 7 cut(s) 17, 882, 885, 966, 1225, 1396, 1412
BseYI CCCAGC 2 cut(s) 1011, 1228
BsgI GTGCAG 2 cut(s) 169, 1395
Bsh1236I CGCG 7 cut(s) 205, 245, 929, 1562, 1573, 1590, 1929
Bsh1285I CGRYCG 2 cut(s) 1577, 1889
BshFI GGCC 9 cut(s) 14, 107, 228, 306, 531, 932, 1010, 1538, 1888
BshNI GGYRCC 1 cut(s) 1584
BshTI ACCGGT 1 cut(s) 507
BsiEI CGRYCG 2 cut(s) 1577, 1889
BsiHKAI GWGCWC 1 cut(s) 164
BsiHKCI CYCGRG 4 cut(s) 215, 455, 637, 1595
BslFI GGGAC 4 cut(s) 382, 793, 1044, 1135
BslI CCNNNNNNNGG 8 cut(s) 189, 195, 196, 405, 886, 939, 1006, 1759
BsmAI GTCTC 9 cut(s) 184, 205, 452, 568, 657, 717, 895, 965, 1522
BsmBI CGTCTC 2 cut(s) 568, 717
BsmFI GGGAC 4 cut(s) 382, 793, 1044, 1135
BsnI GGCC 9 cut(s) 14, 107, 228, 306, 531, 932, 1010, 1538, 1888
Bso31I GGTCTC 4 cut(s) 205, 452, 657, 895
BsoBI CYCGRG 4 cut(s) 215, 455, 637, 1595
Bsp119I TTCGAA 1 cut(s) 1047
Bsp1286I GDGCHC 3 cut(s) 164, 1001, 1709
Bsp143I GATC 4 cut(s) 421, 849, 1879, 1914
Bsp1720I GCTNAGC 1 cut(s) 1238
Bsp19I CCATGG 1 cut(s) 1462
BspANI GGCC 9 cut(s) 14, 107, 228, 306, 531, 932, 1010, 1538, 1888
BspCNI CTCAG 3 cut(s) 277, 291, 1074
BspFNI CGCG 7 cut(s) 205, 245, 929, 1562, 1573, 1590, 1929
BspHI TCATGA 1 cut(s) 1951
BspLI GGNNCC 8 cut(s) 194, 293, 307, 826, 1569, 1586, 1708, 1751
BspMI ACCTGC 3 cut(s) 733, 1746, 1837
BspT104I TTCGAA 1 cut(s) 1047
BspT107I GGYRCC 1 cut(s) 1584
BspTI CTTAAG 1 cut(s) 1451
BspTNI GGTCTC 4 cut(s) 205, 452, 657, 895
BsrDI GCAATG 4 cut(s) 764, 1159, 1513, 1860
BsrFI RCCGGY 3 cut(s) 507, 1502, 1981
BsrI ACTGG 4 cut(s) 259, 1377, 1382, 1753
BssAI RCCGGY 3 cut(s) 507, 1502, 1981
BssMI GATC 4 cut(s) 421, 849, 1879, 1914
BssNI GRCGYC 2 cut(s) 749, 806
BssSI CACGAG 2 cut(s) 1413, 1512
BssT1I CCWWGG 2 cut(s) 9, 1462
Bst2BI CACGAG 2 cut(s) 1413, 1512
Bst2UI CCWGG 4 cut(s) 169, 551, 1359, 1901
Bst4CI ACNGT 6 cut(s) 463, 575, 668, 742, 1346, 1724
Bst6I CTCTTC 2 cut(s) 353, 1163
BstACI GRCGYC 2 cut(s) 749, 806
BstAFI CTTAAG 1 cut(s) 1451
BstBAI YACGTR 1 cut(s) 138
BstBI TTCGAA 1 cut(s) 1047
BstC8I GCNNGC 3 cut(s) 1235, 1861, 1983
BstDEI CTNAG 6 cut(s) 94, 230, 264, 278, 1082, 1238
BstDSI CCRYGG 2 cut(s) 1462, 1571
BstF5I GGATG 5 cut(s) 146, 537, 1186, 1705, 1899
BstFNI CGCG 7 cut(s) 205, 245, 929, 1562, 1573, 1590, 1929
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 4 cut(s) 250, 631, 1445, 1592
BstKTI GATC 4 cut(s) 424, 852, 1882, 1917
BstMAI GTCTC 9 cut(s) 184, 205, 452, 568, 657, 717, 895, 965, 1522
BstMBI GATC 4 cut(s) 421, 849, 1879, 1914
BstMCI CGRYCG 2 cut(s) 1577, 1889
BstNI CCWGG 4 cut(s) 169, 551, 1359, 1901
BstNSI RCATGY 1 cut(s) 1315
BstSLI GKGCMC 1 cut(s) 164
BstUI CGCG 7 cut(s) 205, 245, 929, 1562, 1573, 1590, 1929
BstV1I GCAGC 7 cut(s) 17, 882, 885, 966, 1225, 1396, 1412
BstXI CCANNNNNNTGG 1 cut(s) 1377
BstZI CGGCCG 1 cut(s) 1886
BsuI GTATCC 1 cut(s) 1712
BsuRI GGCC 9 cut(s) 14, 107, 228, 306, 531, 932, 1010, 1538, 1888
BtgI CCRYGG 2 cut(s) 1462, 1571
BtrI CACGTC 3 cut(s) 111, 871, 1636
BtsCI GGATG 5 cut(s) 146, 537, 1186, 1705, 1899
BtsI GCAGTG 1 cut(s) 1878
BtsIMutI CAGTG 2 cut(s) 1370, 1878
BveI ACCTGC 3 cut(s) 733, 1746, 1837
Cac8I GCNNGC 3 cut(s) 1235, 1861, 1983
CaiI CAGNNNCTG 2 cut(s) 1440, 1877
CciI TCATGA 1 cut(s) 1951
CfoI GCGC 4 cut(s) 250, 631, 1445, 1592
Cfr10I RCCGGY 3 cut(s) 507, 1502, 1981
Cfr13I GGNCC 7 cut(s) 305, 877, 1009, 1355, 1380, 1735, 1750
Cfr42I CCGCGG 1 cut(s) 1574
Cfr9I CCCGGG 1 cut(s) 637
CseI GACGC 4 cut(s) 125, 699, 738, 1551
CsiI ACCWGGT 1 cut(s) 1357
Csp6I GTAC 3 cut(s) 1129, 1585, 1810
CspAI ACCGGT 1 cut(s) 507
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviAII CATG 5 cut(s) 1219, 1312, 1463, 1952, 1969
CviQI GTAC 3 cut(s) 1129, 1585, 1810
DdeI CTNAG 6 cut(s) 94, 230, 264, 278, 1082, 1238
DpnI GATC 4 cut(s) 423, 851, 1881, 1916
DpnII GATC 4 cut(s) 421, 849, 1879, 1914
DrdI GACNNNNNNGTC 1 cut(s) 875
DseDI GACNNNNNNGTC 1 cut(s) 875
EaeI YGGCCR 2 cut(s) 930, 1886
EagI CGGCCG 1 cut(s) 1886
Eam1104I CTCTTC 2 cut(s) 353, 1163
EarI CTCTTC 2 cut(s) 353, 1163
EciI GGCGGA 5 cut(s) 482, 934, 1109, 1546, 1731
EclXI CGGCCG 1 cut(s) 1886
Eco130I CCWWGG 2 cut(s) 9, 1462
Eco147I AGGCCT 1 cut(s) 14
Eco24I GRGCYC 2 cut(s) 1001, 1709
Eco31I GGTCTC 4 cut(s) 205, 452, 657, 895
Eco47I GGWCC 5 cut(s) 877, 1355, 1380, 1735, 1750
Eco52I CGGCCG 1 cut(s) 1886
Eco57I CTGAAG 1 cut(s) 305
Eco72I CACGTG 1 cut(s) 138
Eco88I CYCGRG 4 cut(s) 215, 455, 637, 1595
EcoO109I RGGNCCY 1 cut(s) 1355
EcoRII CCWGG 4 cut(s) 167, 549, 1357, 1899
EcoT14I CCWWGG 2 cut(s) 9, 1462
EcoT38I GRGCYC 2 cut(s) 1001, 1709
ErhI CCWWGG 2 cut(s) 9, 1462
Esp3I CGTCTC 2 cut(s) 568, 717
FaeI CATG 5 cut(s) 1222, 1315, 1466, 1955, 1972
FaiI YATR 9 cut(s) 857, 1003, 1220, 1313, 1464, 1641, 1820, 1953, 1970
FalI AAGNNNNNCTT 3 cut(s) 34, 332, 364
FaqI GGGAC 4 cut(s) 382, 793, 1044, 1135
FatI CATG 5 cut(s) 1218, 1311, 1462, 1951, 1968
FauI CCCGC 4 cut(s) 391, 641, 1574, 1930
FbaI TGATCA 1 cut(s) 1879
FblI GTMKAC 3 cut(s) 803, 987, 1302
FokI GGATG 5 cut(s) 133, 524, 1193, 1712, 1906
FriOI GRGCYC 2 cut(s) 1001, 1709
FspBI CTAG 2 cut(s) 1281, 1421
FspI TGCGCA 1 cut(s) 630
GlaI GCGC 4 cut(s) 249, 630, 1444, 1591
GsaI CCCAGC 2 cut(s) 1015, 1232
GsuI CTGGAG 5 cut(s) 533, 989, 1091, 1625, 1682
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 9 cut(s) 14, 107, 228, 306, 531, 932, 1010, 1538, 1888
HgaI GACGC 4 cut(s) 125, 699, 738, 1551
HhaI GCGC 4 cut(s) 250, 631, 1445, 1592
Hin1I GRCGYC 2 cut(s) 749, 806
Hin1II CATG 5 cut(s) 1222, 1315, 1466, 1955, 1972
Hin6I GCGC 4 cut(s) 248, 629, 1443, 1590
HinP1I GCGC 4 cut(s) 248, 629, 1443, 1590
HincII GTYRAC 3 cut(s) 261, 466, 804
HindII GTYRAC 3 cut(s) 261, 466, 804
HindIII AAGCTT 3 cut(s) 962, 1235, 1324
HphI GGTGA 5 cut(s) 458, 1177, 1450, 1774, 1918
Hpy166II GTNNAC 9 cut(s) 162, 261, 466, 786, 804, 988, 1303, 1735, 1776
Hpy8I GTNNAC 9 cut(s) 162, 261, 466, 786, 804, 988, 1303, 1735, 1776
Hpy99I CGWCG 7 cut(s) 115, 808, 872, 997, 1151, 1567, 1922
HpyAV CCTTC 3 cut(s) 25, 1043, 1557
HpyCH4III ACNGT 6 cut(s) 463, 575, 668, 742, 1346, 1724
HpyCH4IV ACGT 8 cut(s) 110, 137, 395, 561, 782, 806, 870, 1635
HpyCH4V TGCA 9 cut(s) 162, 186, 623, 728, 1400, 1412, 1846, 1853, 1863
HpyF3I CTNAG 6 cut(s) 94, 230, 264, 278, 1082, 1238
HpySE526I ACGT 8 cut(s) 110, 137, 395, 561, 782, 806, 870, 1635
Hsp92I GRCGYC 2 cut(s) 749, 806
Hsp92II CATG 5 cut(s) 1222, 1315, 1466, 1955, 1972
HspAI GCGC 4 cut(s) 248, 629, 1443, 1590
KpnI GGTACC 1 cut(s) 1588
KroI GCCGGC 1 cut(s) 1981
KroNI GCCGGC 1 cut(s) 1983
Ksp22I TGATCA 1 cut(s) 1879
KspI CCGCGG 1 cut(s) 1574
Kzo9I GATC 4 cut(s) 421, 849, 1879, 1914
Lsp1109I GCAGC 7 cut(s) 17, 882, 885, 966, 1225, 1396, 1412
LweI GCATC 1 cut(s) 1171
MabI ACCWGGT 1 cut(s) 1357
MaeI CTAG 2 cut(s) 1281, 1421
MaeII ACGT 8 cut(s) 110, 137, 395, 561, 782, 806, 870, 1635
MaeIII GTNAC 4 cut(s) 391, 668, 1346, 1618
MalI GATC 4 cut(s) 423, 851, 1881, 1916
MboI GATC 4 cut(s) 421, 849, 1879, 1914
MboII GAAGA 8 cut(s) 10, 13, 340, 991, 1180, 1210, 1331, 1921
MhlI GDGCHC 3 cut(s) 164, 1001, 1709
MluCI AATT 5 cut(s) 450, 588, 831, 1188, 1395
MlyI GAGTC 6 cut(s) 186, 256, 469, 626, 682, 1985
MmeI TCCRAC 6 cut(s) 93, 154, 177, 1545, 1592, 1611
MroNI GCCGGC 1 cut(s) 1981
MroXI GAANNNNTTC 3 cut(s) 509, 1061, 1205
MseI TTAA 1 cut(s) 1452
MslI CAYNNNNRTG 1 cut(s) 1223
MspA1I CMGCKG 3 cut(s) 1573, 1691, 1868
MspCI CTTAAG 1 cut(s) 1451
MvaI CCWGG 4 cut(s) 169, 551, 1359, 1901
MvnI CGCG 7 cut(s) 205, 245, 929, 1562, 1573, 1590, 1929
NaeI GCCGGC 1 cut(s) 1983
NciI CCSGG 6 cut(s) 190, 197, 638, 639, 696, 1675
NcoI CCATGG 1 cut(s) 1462
NdeII GATC 4 cut(s) 421, 849, 1879, 1914
NgoMIV GCCGGC 1 cut(s) 1981
NlaIII CATG 5 cut(s) 1222, 1315, 1466, 1955, 1972
NlaIV GGNNCC 8 cut(s) 194, 293, 307, 826, 1569, 1586, 1708, 1751
NmeAIII GCCGAG 7 cut(s) 204, 545, 986, 1070, 1767, 1807, 1914
NsbI TGCGCA 1 cut(s) 630
NspI RCATGY 1 cut(s) 1315
NspV TTCGAA 1 cut(s) 1047
PaeR7I CTCGAG 1 cut(s) 215
PagI TCATGA 1 cut(s) 1951
PceI AGGCCT 1 cut(s) 14
PcsI WCGNNNNNNNCGW 1 cut(s) 800
PdiI GCCGGC 1 cut(s) 1983
PdmI GAANNNNTTC 3 cut(s) 509, 1061, 1205
PfeI GAWTC 4 cut(s) 80, 1201, 1924, 1964
PflFI GACNNNGTC 1 cut(s) 1975
PflMI CCANNNNNTGG 1 cut(s) 1759
PfoI TCCNGGA 1 cut(s) 549
PinAI ACCGGT 1 cut(s) 507
PleI GAGTC 6 cut(s) 185, 256, 469, 625, 682, 1984
PmaCI CACGTG 1 cut(s) 138
PmlI CACGTG 1 cut(s) 138
PpsI GAGTC 6 cut(s) 185, 256, 469, 625, 682, 1984
Ppu21I YACGTR 1 cut(s) 138
PpuMI RGGWCCY 1 cut(s) 1355
Psp5II RGGWCCY 1 cut(s) 1355
Psp6I CCWGG 4 cut(s) 167, 549, 1357, 1899
PspCI CACGTG 1 cut(s) 138
PspFI CCCAGC 2 cut(s) 1011, 1228
PspGI CCWGG 4 cut(s) 167, 549, 1357, 1899
PspN4I GGNNCC 8 cut(s) 194, 293, 307, 826, 1569, 1586, 1708, 1751
PspPI GGNCC 7 cut(s) 305, 877, 1009, 1355, 1380, 1735, 1750
PspPPI RGGWCCY 1 cut(s) 1355
PstNI CAGNNNCTG 2 cut(s) 1440, 1877
PsyI GACNNNGTC 1 cut(s) 1975
PvuII CAGCTG 1 cut(s) 1691
RsaI GTAC 3 cut(s) 1130, 1586, 1811
RsaNI GTAC 3 cut(s) 1129, 1585, 1810
RseI CAYNNNNRTG 1 cut(s) 1223
SacII CCGCGG 1 cut(s) 1574
SalI GTCGAC 1 cut(s) 802
SaqAI TTAA 1 cut(s) 1452
Sau3AI GATC 4 cut(s) 421, 849, 1879, 1914
Sau96I GGNCC 7 cut(s) 305, 877, 1009, 1355, 1380, 1735, 1750
SchI GAGTC 6 cut(s) 186, 256, 469, 626, 682, 1985
SduI GDGCHC 3 cut(s) 164, 1001, 1709
SexAI ACCWGGT 1 cut(s) 1357
SfaNI GCATC 1 cut(s) 1171
Sfr274I CTCGAG 1 cut(s) 215
Sfr303I CCGCGG 1 cut(s) 1574
SfuI TTCGAA 1 cut(s) 1047
SgrBI CCGCGG 1 cut(s) 1574
SinI GGWCC 5 cut(s) 877, 1355, 1380, 1735, 1750
SlaI CTCGAG 1 cut(s) 215
SmaI CCCGGG 1 cut(s) 639
SmiMI CAYNNNNRTG 1 cut(s) 1223
SmlI CTYRAG 5 cut(s) 215, 338, 1175, 1261, 1451
SmoI CTYRAG 5 cut(s) 215, 338, 1175, 1261, 1451
Sse9I AATT 5 cut(s) 450, 588, 831, 1188, 1395
SseBI AGGCCT 1 cut(s) 14
SspMI CTAG 2 cut(s) 1281, 1421
StuI AGGCCT 1 cut(s) 14
StyI CCWWGG 2 cut(s) 9, 1462
TaaI ACNGT 6 cut(s) 463, 575, 668, 742, 1346, 1724
TaiI ACGT 8 cut(s) 113, 140, 398, 564, 785, 809, 873, 1638
TasI AATT 5 cut(s) 450, 588, 831, 1188, 1395
TfiI GAWTC 4 cut(s) 80, 1201, 1924, 1964
Tru1I TTAA 1 cut(s) 1452
Tru9I TTAA 1 cut(s) 1452
TscAI CASTG 2 cut(s) 1377, 1878
TseI GCWGC 7 cut(s) 5, 895, 898, 954, 1213, 1400, 1409
TspDTI ATGAA 2 cut(s) 753, 1417
TspGWI ACGGA 3 cut(s) 379, 834, 1988
TspMI CCCGGG 1 cut(s) 637
TspRI CASTG 2 cut(s) 1377, 1878
Tth111I GACNNNGTC 1 cut(s) 1975
Van91I CCANNNNNTGG 1 cut(s) 1759
Vha464I CTTAAG 1 cut(s) 1451
VneI GTGCAC 1 cut(s) 160
VpaK11BI GGWCC 5 cut(s) 877, 1355, 1380, 1735, 1750
XapI RAATTY 2 cut(s) 450, 588
XceI RCATGY 1 cut(s) 1315
XcmI CCANNNNNNNNNTGG 1 cut(s) 1489
XhoI CTCGAG 1 cut(s) 215
XmaI CCCGGG 1 cut(s) 637
XmiI GTMKAC 3 cut(s) 803, 987, 1302
XmnI GAANNNNTTC 3 cut(s) 509, 1061, 1205
XspI CTAG 2 cut(s) 1281, 1421
ZraI GACGTC 1 cut(s) 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.