Rorug05G0089300

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0013779
Physical Location & Seq
Forward (+)
7651839 .. 7682716
30878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0089300.1

Sequence Viewer

Length: 816 bp
ATGGCTGAGAATCATGCAAGTGATGGAAACCATGTCCTCAATGTAGAAGATGACAAACCTTCCTCCAAAACCAACCAAGAATCAAGCAGTAGCTTGCTCTGTGTACTCTTTTTGCAGAAGGTGATTGCTGAGGTGTTGGGGACATACTTCTTGATATTTGCTGGTTGTGGGGCTGTGGTAGTGAATTTCGACACAGATAAGACGGTGTCGTCTCCGGCGGTTGCAATTGTTTGGGGACTGGTTGTCATGGTGATGATTTACTCTGTTGGTCACATCTCCGGTGCCCATTTCAATCCTGCTGTCACAATTGCTTTTGCTACTACTAAGAGGTTTCCATGGAAACAGGTACCCCCTTATATTATTGCTCAAGTCCTTGGATCAACGCTCGCAAGTGGTACCCTCAGGCTCATATTTAACCACCATCAAGACCACTTTGCAGGAACAAGTCCCAGTGGATCTCTATTGCAGACTTTCGTGATTGAGTTCATCATCACATTTTACCTCATGTTTGTTGTTTCTGGTGTGGCCACTGATAACAGAGCGATTGGAGAGCTGGCAGGGATTGCCATTGGCTCCACGGTTCTTCTGAACGTGATGTTTGCAGGTCCAATTACAGGAGCATCAATGAACCCAGCAAGAAGCTTAGGGCCTGTAATTGTCTCAGGCCATTACAAAAACCTGTGGATTTACCTAGTGGCACCAACTCTTGGAGCTGTATGTGGTGCCCTGGTCTACAATGTTATCCGATTTACGGATAAGCCACTGCGAGAGCTCACCAAGACTGGATCTTTCTTGAAAGGCTCAGACCGCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

271

Amino Acids

28.99

Weight (kDa)

7.78

Isoelectric Point (pI)

30.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 35 - 245 1e-64 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 208
Acc36I ACCTGC 1 cut(s) 593
Acc65I GGTACC 2 cut(s) 346, 395
AccB1I GGYRCC 5 cut(s) 281, 346, 395, 697, 722
AccB7I CCANNNNNTGG 1 cut(s) 707
AccI GTMKAC 1 cut(s) 732
AciI CCGC 2 cut(s) 218, 808
AclWI GGATC 3 cut(s) 385, 463, 793
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 1 cut(s) 184
AfaI GTAC 3 cut(s) 105, 348, 397
AfiI CCNNNNNNNGG 3 cut(s) 614, 707, 751
AgsI TTSAA 2 cut(s) 292, 796
AhdI GACNNNNNGTC 1 cut(s) 242
AjnI CCWGG 1 cut(s) 726
AluBI AGCT 5 cut(s) 93, 553, 642, 713, 772
AluI AGCT 5 cut(s) 93, 553, 642, 713, 772
Alw21I GWGCWC 1 cut(s) 774
Alw26I GTCTC 2 cut(s) 216, 664
AlwI GGATC 3 cut(s) 385, 463, 793
AoxI GGCC 3 cut(s) 525, 647, 664
ApoI RAATTY 1 cut(s) 184
Asp718I GGTACC 2 cut(s) 346, 395
AspS9I GGNCC 2 cut(s) 605, 647
AsuHPI GGTGA 3 cut(s) 133, 262, 766
AvaII GGWCC 1 cut(s) 605
AxyI CCTNAGG 1 cut(s) 401
BaeGI GKGCMC 2 cut(s) 286, 727
BalI TGGCCA 1 cut(s) 527
BanI GGYRCC 5 cut(s) 281, 346, 395, 697, 722
BanII GRGCYC 1 cut(s) 774
Bbv12I GWGCWC 1 cut(s) 774
BbvCI CCTCAGC 1 cut(s) 129
BccI CCATC 2 cut(s) 17, 429
BciT130I CCWGG 1 cut(s) 728
BcoDI GTCTC 2 cut(s) 216, 664
BfaI CTAG 1 cut(s) 692
BfuAI ACCTGC 1 cut(s) 593
Bme1390I CCNGG 1 cut(s) 728
Bme18I GGWCC 1 cut(s) 605
BmeRI GACNNNNNGTC 1 cut(s) 242
BmgT120I GGNCC 2 cut(s) 605, 647
BmiI GGNNCC 6 cut(s) 283, 348, 397, 574, 699, 724
BmrFI CCNGG 1 cut(s) 728
BmrI ACTGGG 1 cut(s) 444
BmsI GCATC 1 cut(s) 629
BmuI ACTGGG 1 cut(s) 444
Bpu10I CCTNAGC 2 cut(s) 129, 643
BpuEI CTTGAG 1 cut(s) 351
BsaJI CCNNGG 4 cut(s) 335, 373, 576, 726
BsaWI WCCGGW 1 cut(s) 278
Bsc4I CCNNNNNNNGG 3 cut(s) 614, 707, 751
Bse1I ACTGG 3 cut(s) 243, 450, 787
Bse21I CCTNAGG 1 cut(s) 401
BseBI CCWGG 1 cut(s) 728
BseDI CCNNGG 4 cut(s) 335, 373, 576, 726
BseLI CCNNNNNNNGG 3 cut(s) 614, 707, 751
BseMII CTCAG 4 cut(s) 120, 415, 675, 816
BseNI ACTGG 3 cut(s) 243, 450, 787
BseSI GKGCMC 2 cut(s) 286, 727
BseYI CCCAGC 1 cut(s) 631
BshFI GGCC 3 cut(s) 527, 649, 666
BshNI GGYRCC 5 cut(s) 281, 346, 395, 697, 722
BsiHKAI GWGCWC 1 cut(s) 774
BsiSI CCGG 2 cut(s) 215, 279
BslFI GGGAC 3 cut(s) 154, 249, 432
BslI CCNNNNNNNGG 3 cut(s) 614, 707, 751
BsmAI GTCTC 2 cut(s) 216, 664
BsmBI CGTCTC 1 cut(s) 216
BsmFI GGGAC 3 cut(s) 154, 249, 432
BsnI GGCC 3 cut(s) 527, 649, 666
Bsp1286I GDGCHC 3 cut(s) 286, 727, 774
Bsp143I GATC 3 cut(s) 377, 455, 785
Bsp19I CCATGG 1 cut(s) 335
BspACI CCGC 2 cut(s) 218, 808
BspANI GGCC 3 cut(s) 527, 649, 666
BspCNI CTCAG 4 cut(s) 121, 414, 674, 815
BspLI GGNNCC 6 cut(s) 283, 348, 397, 574, 699, 724
BspMI ACCTGC 1 cut(s) 593
BspPI GGATC 3 cut(s) 385, 463, 793
BspT107I GGYRCC 5 cut(s) 281, 346, 395, 697, 722
BsrI ACTGG 3 cut(s) 243, 450, 787
BssECI CCNNGG 4 cut(s) 335, 373, 576, 726
BssMI GATC 3 cut(s) 377, 455, 785
BssT1I CCWWGG 2 cut(s) 335, 373
Bst2UI CCWGG 1 cut(s) 728
Bst4CI ACNGT 2 cut(s) 205, 580
BstAPI GCANNNNNTGC 1 cut(s) 563
BstC8I GCNNGC 3 cut(s) 95, 387, 555
BstDEI CTNAG 7 cut(s) 6, 129, 324, 401, 643, 661, 802
BstDSI CCRYGG 2 cut(s) 335, 576
BstKTI GATC 3 cut(s) 380, 458, 788
BstMAI GTCTC 2 cut(s) 216, 664
BstMBI GATC 3 cut(s) 377, 455, 785
BstMWI GCNNNNNNNGC 2 cut(s) 563, 807
BstNI CCWGG 1 cut(s) 728
BstSCI CCNGG 1 cut(s) 726
BstSLI GKGCMC 2 cut(s) 286, 727
BstX2I RGATCY 2 cut(s) 455, 785
BstYI RGATCY 2 cut(s) 455, 785
Bsu36I CCTNAGG 1 cut(s) 401
BsuRI GGCC 3 cut(s) 527, 649, 666
BtgI CCRYGG 2 cut(s) 335, 576
BtsI GCAGTG 1 cut(s) 761
BtsIMutI CAGTG 3 cut(s) 457, 528, 761
BveI ACCTGC 1 cut(s) 593
Cac8I GCNNGC 3 cut(s) 95, 387, 555
Cfr13I GGNCC 2 cut(s) 605, 647
Csp6I GTAC 3 cut(s) 104, 347, 396
CviAII CATG 5 cut(s) 14, 32, 247, 336, 505
CviQI GTAC 3 cut(s) 104, 347, 396
DdeI CTNAG 7 cut(s) 6, 129, 324, 401, 643, 661, 802
DpnI GATC 3 cut(s) 379, 457, 787
DpnII GATC 3 cut(s) 377, 455, 785
DrdI GACNNNNNNGTC 1 cut(s) 208
DriI GACNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 525
Eam1105I GACNNNNNGTC 1 cut(s) 242
Ecl136II GAGCTC 1 cut(s) 772
Eco130I CCWWGG 2 cut(s) 335, 373
Eco24I GRGCYC 1 cut(s) 774
Eco47I GGWCC 1 cut(s) 605
Eco53kI GAGCTC 1 cut(s) 772
Eco81I CCTNAGG 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 772
EcoO109I RGGNCCY 1 cut(s) 647
EcoRII CCWGG 1 cut(s) 726
EcoT14I CCWWGG 2 cut(s) 335, 373
EcoT38I GRGCYC 1 cut(s) 774
ErhI CCWWGG 2 cut(s) 335, 373
Esp3I CGTCTC 1 cut(s) 216
FaeI CATG 5 cut(s) 17, 35, 250, 339, 508
FaiI YATR 9 cut(s) 15, 33, 145, 248, 337, 357, 410, 506, 718
FaqI GGGAC 3 cut(s) 154, 249, 432
FatI CATG 5 cut(s) 13, 31, 246, 335, 504
FblI GTMKAC 1 cut(s) 732
FriOI GRGCYC 1 cut(s) 774
FspBI CTAG 1 cut(s) 692
GsaI CCCAGC 1 cut(s) 635
HaeIII GGCC 3 cut(s) 527, 649, 666
HapII CCGG 2 cut(s) 215, 279
Hin1II CATG 5 cut(s) 17, 35, 250, 339, 508
HindIII AAGCTT 1 cut(s) 640
HinfI GANTC 2 cut(s) 10, 80
HpaII CCGG 2 cut(s) 215, 279
HphI GGTGA 3 cut(s) 133, 262, 766
Hpy166II GTNNAC 2 cut(s) 104, 733
Hpy188I TCNGA 3 cut(s) 588, 746, 805
Hpy188III TCNNGA 4 cut(s) 151, 425, 475, 793
Hpy8I GTNNAC 2 cut(s) 104, 733
HpyAV CCTTC 2 cut(s) 69, 112
HpyCH4III ACNGT 2 cut(s) 205, 580
HpyCH4IV ACGT 1 cut(s) 591
HpyCH4V TGCA 6 cut(s) 17, 115, 224, 437, 466, 602
HpyF10VI GCNNNNNNNGC 2 cut(s) 563, 807
HpyF3I CTNAG 7 cut(s) 6, 129, 324, 401, 643, 661, 802
HpySE526I ACGT 1 cut(s) 591
Hsp92II CATG 5 cut(s) 17, 35, 250, 339, 508
KpnI GGTACC 2 cut(s) 350, 399
Kzo9I GATC 3 cut(s) 377, 455, 785
LmnI GCTCC 3 cut(s) 578, 617, 710
LweI GCATC 1 cut(s) 629
MaeI CTAG 1 cut(s) 692
MaeII ACGT 1 cut(s) 591
MaeIII GTNAC 2 cut(s) 269, 301
MalI GATC 3 cut(s) 379, 457, 787
MboI GATC 3 cut(s) 377, 455, 785
MboII GAAGA 2 cut(s) 59, 575
MfeI CAATTG 2 cut(s) 225, 306
MflI RGATCY 2 cut(s) 455, 785
MhlI GDGCHC 3 cut(s) 286, 727, 774
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 5 cut(s) 184, 225, 306, 609, 654
MluNI TGGCCA 1 cut(s) 527
MnlI CCTC 6 cut(s) 47, 73, 124, 321, 410, 512
Mox20I TGGCCA 1 cut(s) 527
MscI TGGCCA 1 cut(s) 527
MseI TTAA 1 cut(s) 414
MslI CAYNNNNRTG 2 cut(s) 18, 251
Msp20I TGGCCA 1 cut(s) 527
MspI CCGG 2 cut(s) 215, 279
MspR9I CCNGG 1 cut(s) 728
MunI CAATTG 2 cut(s) 225, 306
MvaI CCWGG 1 cut(s) 728
MwoI GCNNNNNNNGC 2 cut(s) 563, 807
NcoI CCATGG 1 cut(s) 335
NdeII GATC 3 cut(s) 377, 455, 785
NlaIII CATG 5 cut(s) 17, 35, 250, 339, 508
NlaIV GGNNCC 6 cut(s) 283, 348, 397, 574, 699, 724
NmuCI GTSAC 2 cut(s) 269, 301
PfeI GAWTC 2 cut(s) 10, 80
PflFI GACNNNGTC 1 cut(s) 205
PflMI CCANNNNNTGG 1 cut(s) 707
Psp124BI GAGCTC 1 cut(s) 774
Psp6I CCWGG 1 cut(s) 726
PspFI CCCAGC 1 cut(s) 631
PspGI CCWGG 1 cut(s) 726
PspN4I GGNNCC 6 cut(s) 283, 348, 397, 574, 699, 724
PspPI GGNCC 2 cut(s) 605, 647
PsuI RGATCY 2 cut(s) 455, 785
PsyI GACNNNGTC 1 cut(s) 205
RsaI GTAC 3 cut(s) 105, 348, 397
RsaNI GTAC 3 cut(s) 104, 347, 396
RseI CAYNNNNRTG 2 cut(s) 18, 251
SacI GAGCTC 1 cut(s) 774
SaqAI TTAA 1 cut(s) 414
Sau3AI GATC 3 cut(s) 377, 455, 785
Sau96I GGNCC 2 cut(s) 605, 647
ScrFI CCNGG 1 cut(s) 728
SduI GDGCHC 3 cut(s) 286, 727, 774
SfaNI GCATC 1 cut(s) 629
SinI GGWCC 1 cut(s) 605
SmiMI CAYNNNNRTG 2 cut(s) 18, 251
SmlI CTYRAG 1 cut(s) 366
SmoI CTYRAG 1 cut(s) 366
Sse9I AATT 5 cut(s) 184, 225, 306, 609, 654
SsiI CCGC 2 cut(s) 218, 808
SspMI CTAG 1 cut(s) 692
SstI GAGCTC 1 cut(s) 774
StyD4I CCNGG 1 cut(s) 726
StyI CCWWGG 2 cut(s) 335, 373
TaaI ACNGT 2 cut(s) 205, 580
TaiI ACGT 1 cut(s) 594
TaqI TCGA 1 cut(s) 189
TasI AATT 5 cut(s) 184, 225, 306, 609, 654
TatI WGTACW 1 cut(s) 103
TfiI GAWTC 2 cut(s) 10, 80
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 3 cut(s) 457, 535, 768
TseFI GTSAC 2 cut(s) 269, 301
Tsp45I GTSAC 2 cut(s) 269, 301
TspDTI ATGAA 2 cut(s) 475, 641
TspGWI ACGGA 1 cut(s) 767
TspRI CASTG 3 cut(s) 457, 535, 768
Tth111I GACNNNGTC 1 cut(s) 205
Van91I CCANNNNNTGG 1 cut(s) 707
VpaK11BI GGWCC 1 cut(s) 605
XapI RAATTY 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 732
XspI CTAG 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.