Rorug05G0177400

polyadenylate-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010651
Physical Location & Seq
Forward (+)
16582658 .. 16585531
2874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0177400.1

Sequence Viewer

Length: 1206 bp
ATGGCGACCAAGAGGAGTGTGAGTACCTTGAAGGAGGCTGATTTGAAGGGCAAGAGGGTTTTTGTTAGGGTTGATCTCAATGTTCCTTTGGATGACGACTGTAAGATCACCGATGACACTAGAGTCCGAGCTGCTGTCCCCACCATCAAGTACTTGCTGGGAAATGGTGCCAGAGTCATCCTTGCTTCCCACTTGGGACGTCCAAAGGGTGTCACTCCCAAGTACAGTTTGAAGCCTCTTGTGCCAAGACTGTCTGAGCTCCTTGGAGTGGAGGTTAAAATCGCTAATGATTGCGTCGGTGTGGAAGTTGAAAAGTTGGTTGCTGAGCTTCCAGAGGGAGGAGTTTTGCTCCTTGAGAATGTCAGGTTCTACAAGGAGGAAGAGAAGAATGATCCCGAGTTCGCCAAGAAGCTTGCTTCACTTGCAGATGTCTATGTTAATGATGCTTTTGGCACTGCTCACAGGGCTCATGCATCCACAGAAGGAGTGGCGAAGTACTTGAAGCCTTCTGTTGCTGGATACCTTATGCAGAAGGAACTTGACTATCTTGTTGGTGCCGTGGCAAATCCCAAGAGACCATTTGCTGCTATTGTTGGTGGTTCAAAGGTGTCATCCAAGATTGGAGTCATAGAGTCCTTATTGGCGAAGGTCAACATTCTAGTGTTGGGTGGAGGAATGATCTTTACCTTTTATAAGGCTCAAGGGTATTCAGTTGGATCTTCCCTTGTTGAGGAAGACAAGCTAGATCTTGCAAAATCACTTATGGAGAAGGCAAAGGCCAAGGGAGTTTCTATTCTGCTCCCAACTGATGTGGTTATTGCTGATAAGTTTGCTGCTGATGCCAACAGCAAGGTGGTGCCAGCTTCTGCCATTCCAGATGGTTGGATGGGTTTGGATATCGGACCAGATTCCATCAAGACTTTCAATGAAGCTCTGGACACCACTAAGACTGTTATATGGAATGGACCTATGGGTGTGTTCGAGTTTGAAAAGTTTGCTGCTGGCACCGAGGCGATTGCTAAGAAGCTTGCAGAGCTAAGTGGCAAGGGAGTTACAACTATCATTGGAGGTGGTGACTCTGTAGCTGCTGTGGAGAAGGTTGGCCTTGCTGACAAAATGAGCCACATTTCAACCGGAGGCGGTGCAAGCTTGGAGCTTCTTGAAGGGAAAACCCTCCCTGGAGTCCTTGCTCTTGACGACGCTTAA

Protein Analysis

401

Amino Acids

42.37

Weight (kDa)

6.49

Isoelectric Point (pI)

25.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGK PF00162 10 - 390 8.1e-171 Phosphoglycerate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
AasI GACNNNNNNGTC 1 cut(s) 122
AatII GACGTC 1 cut(s) 202
AccB1I GGYRCC 4 cut(s) 167, 554, 856, 1004
AciI CCGC 1 cut(s) 1140
AclWI GGATC 2 cut(s) 386, 724
AcyI GRCGYC 1 cut(s) 199
AfaI GTAC 4 cut(s) 25, 152, 224, 497
AfiI CCNNNNNNNGG 3 cut(s) 268, 338, 730
AjnI CCWGG 1 cut(s) 1177
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 568
AlwI GGATC 2 cut(s) 386, 724
AlwNI CAGNNNCTG 1 cut(s) 866
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 2 cut(s) 777, 1102
ApeKI GCWGC 5 cut(s) 131, 584, 833, 998, 1085
AspS9I GGNCC 2 cut(s) 902, 965
AsuHPI GGTGA 2 cut(s) 100, 1085
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 2 cut(s) 902, 965
BanI GGYRCC 4 cut(s) 167, 554, 856, 1004
BanII GRGCYC 2 cut(s) 261, 469
BbsI GAAGAC 1 cut(s) 741
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 5 cut(s) 118, 571, 820, 985, 1072
BccI CCATC 4 cut(s) 152, 872, 880, 920
BceAI ACGGC 1 cut(s) 542
BcgI CGANNNNNNTGC 2 cut(s) 998, 1032
BciT130I CCWGG 1 cut(s) 1179
BciVI GTATCC 1 cut(s) 512
BcoDI GTCTC 1 cut(s) 568
BfaI CTAG 3 cut(s) 120, 659, 743
BfmI CTRYAG 1 cut(s) 1080
BfuI GTATCC 1 cut(s) 512
BglII AGATCT 1 cut(s) 745
BisI GCNGC 5 cut(s) 132, 585, 834, 999, 1086
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 5 cut(s) 133, 586, 835, 1000, 1087
BmcAI AGTACT 2 cut(s) 152, 497
Bme1390I CCNGG 1 cut(s) 1179
Bme18I GGWCC 2 cut(s) 902, 965
BmeT110I CYCGRG 1 cut(s) 395
BmgT120I GGNCC 2 cut(s) 902, 965
BmiI GGNNCC 4 cut(s) 169, 556, 858, 1006
BmrFI CCNGG 1 cut(s) 1179
BmsI GCATC 3 cut(s) 433, 482, 829
BpiI GAAGAC 1 cut(s) 741
BplI GAGNNNNNCTC 2 cut(s) 333, 365
BpmI CTGGAG 1 cut(s) 1200
Bpu1102I GCTNAGC 1 cut(s) 324
BpuEI CTTGAG 2 cut(s) 374, 684
BsaHI GRCGYC 1 cut(s) 199
BsaI GGTCTC 1 cut(s) 568
BsaJI CCNNGG 5 cut(s) 262, 558, 780, 1008, 1177
BsaWI WCCGGW 1 cut(s) 1133
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 258, 288
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 338, 730
BseBI CCWGG 1 cut(s) 1179
BseDI CCNNGG 5 cut(s) 262, 558, 780, 1008, 1177
BseGI GGATG 5 cut(s) 97, 177, 473, 611, 891
BseLI CCNNNNNNNGG 3 cut(s) 268, 338, 730
BseMII CTCAG 2 cut(s) 246, 315
BseRI GAGGAG 2 cut(s) 28, 354
BseXI GCAGC 5 cut(s) 118, 571, 820, 985, 1072
BseYI CCCAGC 1 cut(s) 157
BshFI GGCC 2 cut(s) 779, 1104
BshNI GGYRCC 4 cut(s) 167, 554, 856, 1004
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 1 cut(s) 1134
BslFI GGGAC 2 cut(s) 122, 210
BslI CCNNNNNNNGG 3 cut(s) 268, 338, 730
BsmAI GTCTC 1 cut(s) 568
BsmFI GGGAC 2 cut(s) 122, 210
BsnI GGCC 2 cut(s) 779, 1104
Bso31I GGTCTC 1 cut(s) 568
BsoBI CYCGRG 1 cut(s) 395
Bsp1286I GDGCHC 2 cut(s) 261, 469
Bsp143I GATC 6 cut(s) 73, 105, 391, 678, 716, 745
Bsp1720I GCTNAGC 1 cut(s) 324
BspACI CCGC 1 cut(s) 1140
BspANI GGCC 2 cut(s) 779, 1104
BspCNI CTCAG 2 cut(s) 247, 316
BspLI GGNNCC 4 cut(s) 169, 556, 858, 1006
BspPI GGATC 2 cut(s) 386, 724
BspT107I GGYRCC 4 cut(s) 167, 554, 856, 1004
BspTNI GGTCTC 1 cut(s) 568
BssECI CCNNGG 5 cut(s) 262, 558, 780, 1008, 1177
BssMI GATC 6 cut(s) 73, 105, 391, 678, 716, 745
BssNI GRCGYC 1 cut(s) 199
BssT1I CCWWGG 2 cut(s) 262, 780
Bst2UI CCWGG 1 cut(s) 1179
Bst4CI ACNGT 4 cut(s) 101, 227, 252, 952
Bst6I CTCTTC 1 cut(s) 375
BstACI GRCGYC 1 cut(s) 199
BstC8I GCNNGC 5 cut(s) 414, 861, 1003, 1029, 1147
BstDEI CTNAG 5 cut(s) 255, 324, 945, 1020, 1037
BstDSI CCRYGG 1 cut(s) 558
BstENI CCTNNNNNAGG 1 cut(s) 728
BstF5I GGATG 5 cut(s) 97, 177, 473, 611, 891
BstKTI GATC 6 cut(s) 76, 108, 394, 681, 719, 748
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 6 cut(s) 73, 105, 391, 678, 716, 745
BstMWI GCNNNNNNNGC 6 cut(s) 241, 422, 464, 839, 1033, 1146
BstNI CCWGG 1 cut(s) 1179
BstSCI CCNGG 1 cut(s) 1177
BstSFI CTRYAG 1 cut(s) 1080
BstV1I GCAGC 5 cut(s) 118, 571, 820, 985, 1072
BstV2I GAAGAC 1 cut(s) 741
BstX2I RGATCY 2 cut(s) 716, 745
BstXI CCANNNNNNTGG 1 cut(s) 882
BstYI RGATCY 2 cut(s) 716, 745
BsuI GTATCC 1 cut(s) 512
BsuRI GGCC 2 cut(s) 779, 1104
BtgI CCRYGG 1 cut(s) 558
BtsCI GGATG 5 cut(s) 97, 177, 473, 611, 891
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 1 cut(s) 453
Cac8I GCNNGC 5 cut(s) 414, 861, 1003, 1029, 1147
CaiI CAGNNNCTG 1 cut(s) 866
Cfr13I GGNCC 2 cut(s) 902, 965
CseI GACGC 1 cut(s) 283
Csp6I GTAC 4 cut(s) 24, 151, 223, 496
CspCI CAANNNNNGTGG 2 cut(s) 792, 827
CviAII CATG 1 cut(s) 470
CviQI GTAC 4 cut(s) 24, 151, 223, 496
DdeI CTNAG 5 cut(s) 255, 324, 945, 1020, 1037
DpnI GATC 6 cut(s) 75, 107, 393, 680, 718, 747
DpnII GATC 6 cut(s) 73, 105, 391, 678, 716, 745
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 2 cut(s) 262, 780
Eco24I GRGCYC 2 cut(s) 261, 469
Eco31I GGTCTC 1 cut(s) 568
Eco32I GATATC 1 cut(s) 898
Eco47I GGWCC 2 cut(s) 902, 965
Eco53kI GAGCTC 1 cut(s) 259
Eco88I CYCGRG 1 cut(s) 395
EcoICRI GAGCTC 1 cut(s) 259
EcoNI CCTNNNNNAGG 1 cut(s) 728
EcoRII CCWGG 1 cut(s) 1177
EcoRV GATATC 1 cut(s) 898
EcoT14I CCWWGG 2 cut(s) 262, 780
EcoT22I ATGCAT 1 cut(s) 475
EcoT38I GRGCYC 2 cut(s) 261, 469
ErhI CCWWGG 2 cut(s) 262, 780
FaeI CATG 1 cut(s) 473
FaiI YATR 9 cut(s) 435, 471, 527, 629, 693, 764, 956, 958, 971
FaqI GGGAC 2 cut(s) 122, 210
FatI CATG 1 cut(s) 469
Fnu4HI GCNGC 5 cut(s) 132, 585, 834, 999, 1086
FokI GGATG 5 cut(s) 104, 164, 460, 598, 898
FriOI GRGCYC 2 cut(s) 261, 469
Fsp4HI GCNGC 5 cut(s) 132, 585, 834, 999, 1086
FspBI CTAG 3 cut(s) 120, 659, 743
GluI GCNGC 5 cut(s) 132, 585, 834, 999, 1086
GsaI CCCAGC 1 cut(s) 161
GsuI CTGGAG 1 cut(s) 1200
HaeIII GGCC 2 cut(s) 779, 1104
HapII CCGG 1 cut(s) 1134
HgaI GACGC 1 cut(s) 283
Hin1I GRCGYC 1 cut(s) 199
Hin1II CATG 1 cut(s) 473
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HindIII AAGCTT 3 cut(s) 410, 1025, 1147
HinfI GANTC 7 cut(s) 123, 174, 624, 632, 908, 1076, 1182
HpaII CCGG 1 cut(s) 1134
HphI GGTGA 2 cut(s) 100, 1085
Hpy166II GTNNAC 1 cut(s) 652
Hpy188I TCNGA 3 cut(s) 128, 256, 902
Hpy188III TCNNGA 7 cut(s) 332, 395, 875, 916, 935, 1160, 1193
Hpy8I GTNNAC 1 cut(s) 652
Hpy99I CGWCG 2 cut(s) 299, 1202
HpyAV CCTTC 9 cut(s) 25, 40, 476, 516, 526, 640, 763, 1090, 1157
HpyCH4III ACNGT 4 cut(s) 101, 227, 252, 952
HpyCH4IV ACGT 1 cut(s) 199
HpyCH4V TGCA 6 cut(s) 425, 473, 529, 752, 1031, 1145
HpyF10VI GCNNNNNNNGC 6 cut(s) 241, 422, 464, 839, 1033, 1146
HpyF3I CTNAG 5 cut(s) 255, 324, 945, 1020, 1037
HpySE526I ACGT 1 cut(s) 199
Hsp92I GRCGYC 1 cut(s) 199
Hsp92II CATG 1 cut(s) 473
Kzo9I GATC 6 cut(s) 73, 105, 391, 678, 716, 745
LmnI GCTCC 4 cut(s) 264, 354, 804, 1153
Lsp1109I GCAGC 5 cut(s) 118, 571, 820, 985, 1072
LweI GCATC 3 cut(s) 433, 482, 829
MaeI CTAG 3 cut(s) 120, 659, 743
MaeII ACGT 1 cut(s) 199
MaeIII GTNAC 3 cut(s) 211, 1051, 1073
MalI GATC 6 cut(s) 75, 107, 393, 680, 718, 747
MboI GATC 6 cut(s) 73, 105, 391, 678, 716, 745
MboII GAAGA 4 cut(s) 392, 397, 711, 746
MflI RGATCY 2 cut(s) 716, 745
MhlI GDGCHC 2 cut(s) 261, 469
MlyI GAGTC 6 cut(s) 132, 183, 633, 641, 1070, 1191
MmeI TCCRAC 2 cut(s) 694, 863
Mph1103I ATGCAT 1 cut(s) 475
MseI TTAA 3 cut(s) 276, 438, 1204
MslI CAYNNNNRTG 1 cut(s) 659
MspI CCGG 1 cut(s) 1134
MspR9I CCNGG 1 cut(s) 1179
MvaI CCWGG 1 cut(s) 1179
MwoI GCNNNNNNNGC 6 cut(s) 241, 422, 464, 839, 1033, 1146
NdeII GATC 6 cut(s) 73, 105, 391, 678, 716, 745
NlaIII CATG 1 cut(s) 473
NlaIV GGNNCC 4 cut(s) 169, 556, 858, 1006
NmuCI GTSAC 2 cut(s) 211, 1073
NsiI ATGCAT 1 cut(s) 475
PfeI GAWTC 1 cut(s) 908
PkrI GCNGC 5 cut(s) 133, 586, 835, 1000, 1087
PleI GAGTC 6 cut(s) 131, 182, 632, 640, 1070, 1190
PpsI GAGTC 6 cut(s) 131, 182, 632, 640, 1070, 1190
PsiI TTATAA 1 cut(s) 693
Psp124BI GAGCTC 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 1177
PspFI CCCAGC 1 cut(s) 157
PspGI CCWGG 1 cut(s) 1177
PspN4I GGNNCC 4 cut(s) 169, 556, 858, 1006
PspPI GGNCC 2 cut(s) 902, 965
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 2 cut(s) 716, 745
RsaI GTAC 4 cut(s) 25, 152, 224, 497
RsaNI GTAC 4 cut(s) 24, 151, 223, 496
RseI CAYNNNNRTG 1 cut(s) 659
SacI GAGCTC 1 cut(s) 261
SaqAI TTAA 3 cut(s) 276, 438, 1204
SatI GCNGC 5 cut(s) 132, 585, 834, 999, 1086
Sau3AI GATC 6 cut(s) 73, 105, 391, 678, 716, 745
Sau96I GGNCC 2 cut(s) 902, 965
ScaI AGTACT 2 cut(s) 152, 497
SchI GAGTC 6 cut(s) 132, 183, 633, 641, 1070, 1191
ScrFI CCNGG 1 cut(s) 1179
SduI GDGCHC 2 cut(s) 261, 469
SfaNI GCATC 3 cut(s) 433, 482, 829
SfcI CTRYAG 1 cut(s) 1080
SinI GGWCC 2 cut(s) 902, 965
SmiMI CAYNNNNRTG 1 cut(s) 659
SmlI CTYRAG 2 cut(s) 353, 699
SmoI CTYRAG 2 cut(s) 353, 699
SsiI CCGC 1 cut(s) 1140
SspMI CTAG 3 cut(s) 120, 659, 743
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 1 cut(s) 1177
StyI CCWWGG 2 cut(s) 262, 780
TaaI ACNGT 4 cut(s) 101, 227, 252, 952
TaiI ACGT 1 cut(s) 202
TaqI TCGA 1 cut(s) 981
TatI WGTACW 3 cut(s) 150, 222, 495
TfiI GAWTC 1 cut(s) 908
Tru1I TTAA 3 cut(s) 276, 438, 1204
Tru9I TTAA 3 cut(s) 276, 438, 1204
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 2 cut(s) 211, 1073
TseI GCWGC 5 cut(s) 131, 584, 833, 998, 1085
Tsp45I GTSAC 2 cut(s) 211, 1073
TspDTI ATGAA 1 cut(s) 942
TspRI CASTG 1 cut(s) 460
VpaK11BI GGWCC 2 cut(s) 902, 965
XagI CCTNNNNNAGG 1 cut(s) 728
XcmI CCANNNNNNNNNTGG 2 cut(s) 484, 850
XspI CTAG 3 cut(s) 120, 659, 743
ZraI GACGTC 1 cut(s) 200
ZrmI AGTACT 2 cut(s) 152, 497
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.