Rorug05G0198000

Cytokinin riboside 5'-monophosphate phosphoribohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006355
Physical Location & Seq
Reverse (-)
19638911 .. 19640443
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0198000.1

Sequence Viewer

Length: 1332 bp
ATGAAGTTTCAAGTGGAGGTTCTCTCAACTGAGACGATCAAACCCTCATCTCCAACCCCTGACCACCTCCGCCACCACAAGCTCTCCTATCTCGACCAAATTCAGCCCCCAGTCTTCATGCCCATGGTCCTCTTCTACCCAACAGAACCCAACCACAACGACCTGGACCTCATTTCCATCCACGAACAGCGTTGCAGCAAAATCAAGAAGGCCTTATCCGACACCTTAGTCAAGTTCTACCCACTAGCCGGAAGAGTTCACGGAACCCAATACGTGGATTGCAACGACGAAGGAGCCTACTACGTCGAAGCCAAGACCAACTGCAAAATCTCTGACATCATCGACAACCCAAACCCTAAGGACTTCAACAAGTTCCTCCCTTTCGAGCTTGACGCAGCTCATGAGCTTCCCGTGTGCTTCCAGGTCACCTTCTTCGCCTGCGGCGGCATGAGCATCGCCATGGGGATGTCCCACAAGGTCGGGGACGCCCTCTCCTACTTCACGTTTCTCAATGGCCTGGCCGCTGAGGCTCGTGGGGAGGGCAACGATATTCGCCATCCCGATTTCATCTCAGACAAGTACTTCCCCCAAAAGAATTTATCCGGATTCTTCCAACACCGTAGCGGGATCATAAAGCAAAACATCTCAACAAAACGCTTCGTCTTCGACGCTCCAATGGTTCAGGCCATCCGAGCCAAATGCGTCGACATGTCCAGACGCCCTACTCGCGTTGAAGCCCTATCAGCCTTCATATGGAGCTGCTACATAGCATCATGCACCGGCATTCACCAAGATGGTGTCACCACCGACGCGATGTACACTGTTTCTCATGCTGTGAACCTCCGCACGAGAATGGAGCCACCACTTCCGGAAAACACATTCGGAAACGTGACCCGAACCGCCATTGCTGTGCCGCCCCTACCCGTTGACCCGAAAGATGGGGCGCTTCATGGAGTGGTTAATCACGTCAGGGAGGCCATCAAGCAAGTAGACAAAGAGTACATAAAGAAACTGCAAGAGGGTGACGGACACTTGAGCTTCTTGAGAAGCCGTACGGAGGAGGTGAAACGCGGAGAGGTGGTGTCGTTTAGCTTCACGAGTCTGTGTAGGTTTCCGATCTACGACGCTGATTTTGGATGGGGAAAGCCTGTTTATGTTGGTTCTGCCAGCTTTACGTTCAAGAACTTGGTTAGTTTCTTTGACACTAACGTAGGGGACGGAGTTGAGGCGTGGATCAATCTGTTGGAGGAAGACATGAAAAAATTCGAGGCAGACGAGGAGCTTCTCAAATATGTTTCGCCCGCCCCGAGTTCGAAGAATGCCAACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

443

Amino Acids

49.81

Weight (kDa)

6.32

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 3 - 428 5.9e-80 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 227
AccB7I CCANNNNNTGG 1 cut(s) 274
AccI GTMKAC 2 cut(s) 705, 990
AccII CGCG 3 cut(s) 729, 812, 1071
AccIII TCCGGA 2 cut(s) 602, 868
AclWI GGATC 2 cut(s) 635, 1241
AcoI YGGCCR 1 cut(s) 519
AcsI RAATTY 3 cut(s) 99, 595, 1262
AcyI GRCGYC 2 cut(s) 486, 718
AfaI GTAC 4 cut(s) 581, 818, 1001, 1054
AfiI CCNNNNNNNGG 5 cut(s) 248, 274, 753, 938, 1057
AflIII ACRYGT 1 cut(s) 708
AgsI TTSAA 4 cut(s) 11, 367, 734, 1180
AjiI CACGTC 1 cut(s) 967
AjnI CCWGG 3 cut(s) 162, 420, 516
AluBI AGCT 9 cut(s) 82, 388, 398, 406, 759, 1038, 1092, 1170, 1282
AluI AGCT 9 cut(s) 82, 388, 398, 406, 759, 1038, 1092, 1170, 1282
Alw26I GTCTC 1 cut(s) 26
AlwI GGATC 2 cut(s) 635, 1241
Ama87I CYCGRG 1 cut(s) 1306
Aor13HI TCCGGA 2 cut(s) 602, 868
AoxI GGCC 5 cut(s) 210, 514, 519, 684, 975
ApeKI GCWGC 3 cut(s) 195, 395, 759
ApoI RAATTY 3 cut(s) 99, 595, 1262
ArsI GACNNNNNNTTYG 2 cut(s) 1115, 1147
Asp700I GAANNNNTTC 1 cut(s) 1262
AspLEI GCGC 1 cut(s) 946
AspS9I GGNCC 2 cut(s) 127, 166
AsuHPI GGTGA 5 cut(s) 418, 779, 793, 1034, 1075
AsuII TTCGAA 1 cut(s) 1313
AvaI CYCGRG 1 cut(s) 1306
AvaII GGWCC 2 cut(s) 127, 166
AxyI CCTNAGG 1 cut(s) 357
BauI CACGAG 3 cut(s) 531, 847, 1096
BbsI GAAGAC 3 cut(s) 106, 655, 1257
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 3 cut(s) 207, 407, 746
BccI CCATC 7 cut(s) 185, 564, 695, 788, 932, 986, 1131
BceAI ACGGC 1 cut(s) 1035
BcgI CGANNNNNNTGC 4 cut(s) 436, 470, 681, 715
BciT130I CCWGG 3 cut(s) 164, 422, 518
BcoDI GTCTC 1 cut(s) 26
BfaI CTAG 1 cut(s) 245
BfoI RGCGCY 1 cut(s) 947
BglI GCCNNNNNGGC 1 cut(s) 527
BisI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
BlsI GCNGC 7 cut(s) 197, 397, 443, 446, 523, 761, 915
BmcAI AGTACT 1 cut(s) 581
Bme1390I CCNGG 3 cut(s) 164, 422, 518
Bme18I GGWCC 2 cut(s) 127, 166
BmeT110I CYCGRG 1 cut(s) 1306
BmgBI CACGTC 1 cut(s) 967
BmgT120I GGNCC 2 cut(s) 127, 166
BmiI GGNNCC 3 cut(s) 265, 295, 858
BmrFI CCNGG 3 cut(s) 164, 422, 518
BmrI ACTGGG 1 cut(s) 104
BmsI GCATC 2 cut(s) 462, 779
BmuI ACTGGG 1 cut(s) 104
BpiI GAAGAC 3 cut(s) 106, 655, 1257
BplI GAGNNNNNCTC 2 cut(s) 8, 40
Bpu10I CCTNAGC 1 cut(s) 525
Bpu14I TTCGAA 1 cut(s) 1313
BpuEI CTTGAG 2 cut(s) 1054, 1063
BsaAI YACGTR 1 cut(s) 274
BsaHI GRCGYC 2 cut(s) 486, 718
BsaJI CCNNGG 2 cut(s) 123, 459
BsaWI WCCGGW 2 cut(s) 602, 868
BsaXI ACNNNNNCTCC 6 cut(s) 68, 98, 476, 506, 1065, 1095
Bsc4I CCNNNNNNNGG 5 cut(s) 248, 274, 753, 938, 1057
Bse118I RCCGGY 1 cut(s) 779
Bse1I ACTGG 1 cut(s) 110
Bse21I CCTNAGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 903
BseAI TCCGGA 2 cut(s) 602, 868
BseBI CCWGG 3 cut(s) 164, 422, 518
BseDI CCNNGG 2 cut(s) 123, 459
BseGI GGATG 5 cut(s) 177, 471, 556, 687, 1142
BseLI CCNNNNNNNGG 5 cut(s) 248, 274, 753, 938, 1057
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 3 cut(s) 21, 516, 585
BseNI ACTGG 1 cut(s) 110
BseRI GAGGAG 2 cut(s) 1073, 1292
BseXI GCAGC 3 cut(s) 207, 407, 746
Bsh1236I CGCG 3 cut(s) 729, 812, 1071
BshFI GGCC 5 cut(s) 212, 516, 521, 686, 977
BsiHKCI CYCGRG 1 cut(s) 1306
BsiSI CCGG 4 cut(s) 249, 603, 780, 869
BsiWI CGTACG 1 cut(s) 1052
BslFI GGGAC 3 cut(s) 454, 497, 1229
BslI CCNNNNNNNGG 5 cut(s) 248, 274, 753, 938, 1057
BsmAI GTCTC 1 cut(s) 26
BsmBI CGTCTC 1 cut(s) 26
BsmFI GGGAC 3 cut(s) 454, 497, 1229
BsmI GAATGC 2 cut(s) 783, 1324
BsnI GGCC 5 cut(s) 212, 516, 521, 686, 977
BsoBI CYCGRG 1 cut(s) 1306
Bsp119I TTCGAA 1 cut(s) 1313
Bsp13I TCCGGA 2 cut(s) 602, 868
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 4 cut(s) 36, 627, 1116, 1233
Bsp19I CCATGG 2 cut(s) 123, 459
BspANI GGCC 5 cut(s) 212, 516, 521, 686, 977
BspCNI CTCAG 3 cut(s) 22, 517, 584
BspEI TCCGGA 2 cut(s) 602, 868
BspFNI CGCG 3 cut(s) 729, 812, 1071
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 3 cut(s) 265, 295, 858
BspPI GGATC 2 cut(s) 635, 1241
BspT104I TTCGAA 1 cut(s) 1313
BsrDI GCAATG 1 cut(s) 903
BsrFI RCCGGY 1 cut(s) 779
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 1 cut(s) 110
BssAI RCCGGY 1 cut(s) 779
BssECI CCNNGG 2 cut(s) 123, 459
BssMI GATC 4 cut(s) 36, 627, 1116, 1233
BssNI GRCGYC 2 cut(s) 486, 718
BssSI CACGAG 3 cut(s) 531, 847, 1096
BssT1I CCWWGG 2 cut(s) 123, 459
Bst2BI CACGAG 3 cut(s) 531, 847, 1096
Bst2UI CCWGG 3 cut(s) 164, 422, 518
Bst4CI ACNGT 2 cut(s) 620, 823
Bst6I CTCTTC 2 cut(s) 137, 247
BstACI GRCGYC 2 cut(s) 486, 718
BstAUI TGTACA 1 cut(s) 816
BstBAI YACGTR 1 cut(s) 274
BstBI TTCGAA 1 cut(s) 1313
BstC8I GCNNGC 3 cut(s) 439, 1168, 1302
BstDEI CTNAG 5 cut(s) 30, 226, 357, 525, 571
BstDSI CCRYGG 2 cut(s) 123, 459
BstEII GGTNACC 1 cut(s) 424
BstF5I GGATG 5 cut(s) 177, 471, 556, 687, 1142
BstFNI CGCG 3 cut(s) 729, 812, 1071
BstH2I RGCGCY 1 cut(s) 947
BstHHI GCGC 1 cut(s) 946
BstKTI GATC 4 cut(s) 39, 630, 1119, 1236
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 4 cut(s) 36, 627, 1116, 1233
BstMWI GCNNNNNNNGC 5 cut(s) 450, 527, 692, 726, 743
BstNI CCWGG 3 cut(s) 164, 422, 518
BstNSI RCATGY 1 cut(s) 712
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 3 cut(s) 162, 420, 516
BstUI CGCG 3 cut(s) 729, 812, 1071
BstV1I GCAGC 3 cut(s) 207, 407, 746
BstV2I GAAGAC 3 cut(s) 106, 655, 1257
Bsu36I CCTNAGG 1 cut(s) 357
BsuRI GGCC 5 cut(s) 212, 516, 521, 686, 977
BtgI CCRYGG 2 cut(s) 123, 459
BtgZI GCGATG 2 cut(s) 439, 827
BtrI CACGTC 1 cut(s) 967
BtsCI GGATG 5 cut(s) 177, 471, 556, 687, 1142
BtsIMutI CAGTG 1 cut(s) 819
Cac8I GCNNGC 3 cut(s) 439, 1168, 1302
CciI TCATGA 1 cut(s) 400
CfoI GCGC 1 cut(s) 946
Cfr10I RCCGGY 1 cut(s) 779
Cfr13I GGNCC 2 cut(s) 127, 166
CseI GACGC 7 cut(s) 401, 494, 677, 691, 726, 818, 1133
Csp6I GTAC 4 cut(s) 580, 817, 1000, 1053
CviQI GTAC 4 cut(s) 580, 817, 1000, 1053
DdeI CTNAG 5 cut(s) 30, 226, 357, 525, 571
DpnI GATC 4 cut(s) 38, 629, 1118, 1235
DpnII GATC 4 cut(s) 36, 627, 1116, 1233
DrdI GACNNNNNNGTC 1 cut(s) 227
DseDI GACNNNNNNGTC 1 cut(s) 227
EaeI YGGCCR 1 cut(s) 519
Eam1104I CTCTTC 2 cut(s) 137, 247
EarI CTCTTC 2 cut(s) 137, 247
EciI GGCGGA 1 cut(s) 59
Eco130I CCWWGG 2 cut(s) 123, 459
Eco147I AGGCCT 1 cut(s) 212
Eco47I GGWCC 2 cut(s) 127, 166
Eco81I CCTNAGG 1 cut(s) 357
Eco88I CYCGRG 1 cut(s) 1306
Eco91I GGTNACC 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 3 cut(s) 162, 420, 516
EcoT14I CCWWGG 2 cut(s) 123, 459
ErhI CCWWGG 2 cut(s) 123, 459
Esp3I CGTCTC 1 cut(s) 26
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 3 cut(s) 454, 497, 1229
FauI CCCGC 2 cut(s) 617, 1309
FauNDI CATATG 1 cut(s) 752
FblI GTMKAC 2 cut(s) 705, 990
Fnu4HI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
FokI GGATG 5 cut(s) 164, 478, 543, 674, 1149
Fsp4HI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
FspBI CTAG 1 cut(s) 245
GlaI GCGC 1 cut(s) 945
GluI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
HaeII RGCGCY 1 cut(s) 947
HaeIII GGCC 5 cut(s) 212, 516, 521, 686, 977
HapII CCGG 4 cut(s) 249, 603, 780, 869
HgaI GACGC 7 cut(s) 401, 494, 677, 691, 726, 818, 1133
HhaI GCGC 1 cut(s) 946
Hin1I GRCGYC 2 cut(s) 486, 718
Hin6I GCGC 1 cut(s) 944
HinP1I GCGC 1 cut(s) 944
HincII GTYRAC 2 cut(s) 706, 928
HindII GTYRAC 2 cut(s) 706, 928
HinfI GANTC 2 cut(s) 606, 1099
HpaII CCGG 4 cut(s) 249, 603, 780, 869
HphI GGTGA 5 cut(s) 418, 779, 793, 1034, 1075
Hpy166II GTNNAC 6 cut(s) 259, 706, 819, 838, 928, 991
Hpy188I TCNGA 6 cut(s) 220, 334, 574, 692, 884, 1116
Hpy8I GTNNAC 6 cut(s) 259, 706, 819, 838, 928, 991
Hpy99I CGWCG 6 cut(s) 290, 308, 671, 707, 812, 1127
HpyAV CCTTC 4 cut(s) 202, 284, 439, 757
HpyCH4III ACNGT 2 cut(s) 620, 823
HpyCH4IV ACGT 7 cut(s) 273, 303, 503, 888, 966, 1175, 1209
HpyCH4V TGCA 5 cut(s) 195, 282, 324, 777, 1015
HpyF10VI GCNNNNNNNGC 5 cut(s) 450, 527, 692, 726, 743
HpyF3I CTNAG 5 cut(s) 30, 226, 357, 525, 571
HpySE526I ACGT 7 cut(s) 273, 303, 503, 888, 966, 1175, 1209
Hsp92I GRCGYC 2 cut(s) 486, 718
HspAI GCGC 1 cut(s) 944
Kpn2I TCCGGA 2 cut(s) 602, 868
Kzo9I GATC 4 cut(s) 36, 627, 1116, 1233
LmnI GCTCC 5 cut(s) 293, 676, 756, 856, 1279
Lsp1109I GCAGC 3 cut(s) 207, 407, 746
LweI GCATC 2 cut(s) 462, 779
MaeI CTAG 1 cut(s) 245
MaeII ACGT 7 cut(s) 273, 303, 503, 888, 966, 1175, 1209
MaeIII GTNAC 4 cut(s) 424, 799, 889, 1022
MalI GATC 4 cut(s) 38, 629, 1118, 1235
MboI GATC 4 cut(s) 36, 627, 1116, 1233
MboII GAAGA 8 cut(s) 106, 124, 264, 424, 601, 655, 1262, 1327
MluCI AATT 3 cut(s) 99, 595, 1262
MlyI GAGTC 1 cut(s) 1108
MmeI TCCRAC 4 cut(s) 77, 243, 637, 1224
MroI TCCGGA 2 cut(s) 602, 868
MroXI GAANNNNTTC 1 cut(s) 1262
MseI TTAA 1 cut(s) 960
MslI CAYNNNNRTG 6 cut(s) 122, 458, 464, 792, 851, 908
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 4 cut(s) 249, 603, 780, 869
MspR9I CCNGG 3 cut(s) 164, 422, 518
Mva1269I GAATGC 2 cut(s) 783, 1324
MvaI CCWGG 3 cut(s) 164, 422, 518
MvnI CGCG 3 cut(s) 729, 812, 1071
MwoI GCNNNNNNNGC 5 cut(s) 450, 527, 692, 726, 743
NcoI CCATGG 2 cut(s) 123, 459
NdeI CATATG 1 cut(s) 752
NdeII GATC 4 cut(s) 36, 627, 1116, 1233
NlaIV GGNNCC 3 cut(s) 265, 295, 858
NmuCI GTSAC 4 cut(s) 424, 799, 889, 1022
NspI RCATGY 1 cut(s) 712
NspV TTCGAA 1 cut(s) 1313
PagI TCATGA 1 cut(s) 400
PceI AGGCCT 1 cut(s) 212
PciI ACATGT 1 cut(s) 708
PcsI WCGNNNNNNNCGW 2 cut(s) 1272, 1304
PctI GAATGC 2 cut(s) 783, 1324
PdmI GAANNNNTTC 1 cut(s) 1262
PfeI GAWTC 1 cut(s) 606
Pfl23II CGTACG 1 cut(s) 1052
PflMI CCANNNNNTGG 1 cut(s) 274
PkrI GCNGC 7 cut(s) 197, 397, 443, 446, 523, 761, 915
PleI GAGTC 1 cut(s) 1107
PpsI GAGTC 1 cut(s) 1107
Ppu21I YACGTR 1 cut(s) 274
PscI ACATGT 1 cut(s) 708
Psp6I CCWGG 3 cut(s) 162, 420, 516
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 3 cut(s) 162, 420, 516
PspLI CGTACG 1 cut(s) 1052
PspN4I GGNNCC 3 cut(s) 265, 295, 858
PspPI GGNCC 2 cut(s) 127, 166
RsaI GTAC 4 cut(s) 581, 818, 1001, 1054
RsaNI GTAC 4 cut(s) 580, 817, 1000, 1053
RseI CAYNNNNRTG 6 cut(s) 122, 458, 464, 792, 851, 908
SalI GTCGAC 1 cut(s) 704
SaqAI TTAA 1 cut(s) 960
SatI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
Sau3AI GATC 4 cut(s) 36, 627, 1116, 1233
Sau96I GGNCC 2 cut(s) 127, 166
ScaI AGTACT 1 cut(s) 581
SchI GAGTC 1 cut(s) 1108
ScrFI CCNGG 3 cut(s) 164, 422, 518
SfaNI GCATC 2 cut(s) 462, 779
SfuI TTCGAA 1 cut(s) 1313
SinI GGWCC 2 cut(s) 127, 166
SmiMI CAYNNNNRTG 6 cut(s) 122, 458, 464, 792, 851, 908
SmlI CTYRAG 2 cut(s) 1033, 1042
SmoI CTYRAG 2 cut(s) 1033, 1042
Sse9I AATT 3 cut(s) 99, 595, 1262
SseBI AGGCCT 1 cut(s) 212
SspMI CTAG 1 cut(s) 245
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 3 cut(s) 162, 420, 516
StyI CCWWGG 2 cut(s) 123, 459
TaaI ACNGT 2 cut(s) 620, 823
TaiI ACGT 7 cut(s) 276, 306, 506, 891, 969, 1178, 1212
TaqI TCGA 8 cut(s) 93, 306, 342, 384, 666, 705, 1266, 1313
TasI AATT 3 cut(s) 99, 595, 1262
TatI WGTACW 3 cut(s) 579, 816, 999
TauI GCSGC 4 cut(s) 444, 447, 524, 916
TfiI GAWTC 1 cut(s) 606
Tru1I TTAA 1 cut(s) 960
Tru9I TTAA 1 cut(s) 960
TscAI CASTG 1 cut(s) 826
TseFI GTSAC 4 cut(s) 424, 799, 889, 1022
TseI GCWGC 3 cut(s) 195, 395, 759
Tsp45I GTSAC 4 cut(s) 424, 799, 889, 1022
TspDTI ATGAA 6 cut(s) 17, 106, 556, 739, 938, 1271
TspGWI ACGGA 4 cut(s) 276, 1041, 1070, 1233
TspRI CASTG 1 cut(s) 826
Van91I CCANNNNNTGG 1 cut(s) 274
VpaK11BI GGWCC 2 cut(s) 127, 166
XapI RAATTY 3 cut(s) 99, 595, 1262
XceI RCATGY 1 cut(s) 712
XmiI GTMKAC 2 cut(s) 705, 990
XmnI GAANNNNTTC 1 cut(s) 1262
XspI CTAG 1 cut(s) 245
ZrmI AGTACT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.