Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0009261

Physical Location & Seq

Genomic Coordinates Forward (+)

13833384 .. 14065534

Gene Structure 232151 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0104900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3135 bp

ATGGATCGGAGCAAACCTCAATTCGGAGCCTCTTCCTCTTCATCTTCTTCTCCCACCCATTCGTTCACACACGATGTCTTTCTGAGCTTCAGAGGTGCGGATACGCGCAACAATTTCACAGGCCATTTGTACCGCAATTTGGTCAACAAGGGAATTAACACATTCATAGATGATGGGCTTCCAAAAGGAGAAGATATATCACAAGAGCTTCTCGAAGTAATTGAGAGATCAAAGATTTCCGTCCTTGTATTTTCTGCGAACTATGCATCTTCAAAGTGGTGCTTGGATGAACTTGTCAAGATCATTCAATGTAAAGAATCCAAGCACCAAATGGTTTACCCAATTTTCTACAAGGTAGATCCGTCGGAGATACGATACCAGAAGGGCCGGGTTGGTGAGGCAATTGCTCGCCTTAGCAAACACAAGGAGAACTTGGAGAAGGTGGAGAATTGGAAGGCAGCTCTTAGAAAAGCAGCAACTTTCTCTGGGTGGCACATCTCAGAAGGAGGGCATGAAGCGAATGTCATTGATGAAATTGTTAAAGAGATATCAACCAAAATAATGAAATACACCCCTTTAAATGTGGCAACATATCCTGTTGGAATAGAATCTCGTGTAGAAGACATACTTAAACTCTTAAATGTTGCGGAAGACGATCCTCACATGATAGGGATATGGGGAATTGGTGGAATAGGAAAGACAACACTTGCAAAAGCTGTTTATAATTCAATTAGGCATAAGTTTGAACATAGCTGCTTCCTGGCAAATGTTAGAGAAGAATCATTTTCACATGGAGGTCTTCTGAAGCTACAAAACAATTTTCTTTCTAAGATTATAGAGAGAAATCCACCTAATGTGACCAATGTGGATGAAGGAATCACTCAGCTGAAGCAAAAACTAAGCCAGAAAAGGGTTCTCTTAGTTCTTGATGATGTGGACCGCTTGGATCAGTTAAAAAAACTTGCTGGAGGGTGTGATTGGTTTGGTCCAAGAAGTAGAATTATTATAACAACAAGAGAAATGAATTGCCTAAGGGCTCATAACATCAATCCAATATATGAGGCCAAGGAATTAAATGATCAAGAAGCTTTAGAGCTCTTCAATTTCCATGCCTTCAAAGGAAATATACGTATGGATGATTTCTTTAACCTTTTAAATGGTGTAATACGTTATGCTAAAGGGATTCCGTTAGCTTTGGAAGTTTTGGGATCAGATCTATGTAGTAAAAATAAGGATGAGTGGAAAGATGCATTAGAGTATTACAACAAATATCCTAACCAAGAGATTCAACAAGCTCTCAAAAGAAGTTATGATGCATTGGAGGATCGGATGAAGGAAGTTTTTCTTCATATTGCATGTTTCTTTAAAGGTCACGAACAAAGCTATGTGATAAATGTCCTACAAAGCTGTGACCTCAATCCTATGTATAGTCTCAAAGTCCTCGTAGAAAAGGCCTTAATAAAAATTGAGAAAGGCAATAATATTTGGATGCATGACCTTCTAGAAGACATGGGAAAAGAAATTGTTCGACAAGAGTCTCCTGATGAGAAAGGACAACGCAGCAGACTGTGGCTATATGAGGATATTTGCGAAGTCTTTGAGGAAAACAAAGGAACGGATAAAATCAAAGCCATCGTGTTGAGAAGAGTTAAGCCTTCACAAATGATATGGTTGAACCGTGGAAGCTTCACAAACTTGAAAAATCTTCAAATTTTTAAAGTCGAATATGGGAAGTATAGAGGAGGGCATGTGGATTATCTCCCCAACCAGTTGAGGGTCCTTGACTGGATGGAGTGTTCATTACGATCGTTGCCAAAGGGTTTTAATCCGACGAAACTCAGTATGCTGAGCATGGGTGGGCCCTGCACGACTCCACTAATCAAGGGATTGCAGAATCTGCGATATTTGAAATCAATCAAATTGCGTGGCTGTGATGGTCTAACCAGAATTCCGGACTTGTCTGGATTAACAAGCTTAAAGTATTTGAAGTTGTCTGAATGTAAAGATTTAGTTGAAGTTCATCCTTCGGTTGGCCGTCTCGATAAGCTCGTCTATTTGGGAATCAGATATTGTAAAAAGCTGCAGATGTTTCAAGAGAGGATCAACTTGAAATCTTTGGAAACTCTGGATCTCTCTGATTGCCCGCTTGAGTGCTTCCCTGAAATTGAGGGAGACATGAAGTCTTTGAATCGCATGGATCTAAGTTTCACTGACATCAAAGAGTTACCTTGCTCAGTTGGAAATCTCACTGGCCTTAAATCCTTGTCTCTAAGTTGGTGTAAAAATCTTACAAATGTACCATCGAGCATTTTCTATGAATTGCAACGTCTGGAGAGGCTTGATCTAGGGTACTGCTCTAAGCTTGTTACATTTCCAACAAAGTCGGAATCACTTCCTCCACCGGTCTTTTCAACTGACCTGACGAGATTACAAGTTAAACTGTACAAATGCTACAGGCTTGAAGAAATTTCAGAATTCCCACGAGAAATAGATTCCTTGGATGTGTGGGGTAGTGGGTCATTGGAAAGAATTTCAAAGTTGTCAAAAATTTTGGAGGGTAAAGATTCAAAAATGTTCCGTCAGTTGGACTTGGCTGACTGCCAGAGACTCTGCGACAATCTGGCTCGCAGAGTGGTAACCAAAGAATTGATGGGAGAGGCAGACAAATTGGCGGCTCTGTTGACTCTTTTTTTCTCTTGTCCGAAATCTGAAGAATTCAAAGTAAGATTTCCTGCACGTGCTCCGCTTCCAAACTGGCTCACCTGCCGTCGGGATGTGAATAGTGAAGAGCATGAGTTTTGCATTGAACTTCCTCAAAACTCCAATTGGGACAAGAAAGGGTTGGCTCTCTGTATTCAATCGAATTCTGATGACCTCACTCGTTGTGTTTACATCAATGGAATAGAGTTTGATGAGAAACGCATGTTTGGGCCTGAAGTGTGGGTGCATTATATTCCATTGGTTACAGTAATAAGGAGGTTGAGTGAGTGTGGGATGCCGCCACCAGATCGGTTTCGAGTTATGTTTCGTCTTAAAATGAATTATATTGAAAGACAAATGATCCCAAGAGAAATGCCTTCTGGGGAAAGCTGGGGAGTCCACCTGATCGAAGATTTGGAAGCAGAAGGCCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005802 GO:0006810 GO:0006869 GO:0008150 GO:0008289 GO:0010876 GO:0012505 GO:0017089 GO:0031974 GO:0031981 GO:0031984 GO:0032365 GO:0033036 GO:0033218 GO:0035091 GO:0035621 GO:0035627 GO:0042886 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046625 GO:0046836 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051861 GO:0070013 GO:0070273 GO:0071702 GO:0071705 GO:0097001 GO:0097367 GO:0098791 GO:1901264 GO:1901981

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GLTP

Protein Analysis

1044

Amino Acids

119.94

Weight (kDa)

8.16

Isoelectric Point (pI)

43.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	24 - 193	1e-53	TIR domain
TIR_2	PF13676	26 - 121	3.2e-14	TIR domain
NB-ARC	PF00931	203 - 374	1.7e-19	NB-ARC domain
ATPase_2	PF01637	205 - 320	6.7e-08	ATPase domain predominantly from Archaea
WHD_ROQ1	PF23282	443 - 513	3e-20	Disease resistance protein Roq1-like, winged-helix domain
LRR_14	PF23598	638 - 780	1.1e-10	Leucine-rich repeat region
LRR_13	PF23286	703 - 788	2.6e-10	Disease resistance protein RPS4B-like, leucine-rich repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009261)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21360 AT1G21360 AT1G21360 AT3G21260 AT3G21260 AT3G21260
fragaria_vesca	FvH4_2g14560
malus_domestica	MD05G1017200.v1.1 MD10G1017400.v1.1
prunus_persica	Prupe.8G021000_v2.0.a1
pyrus_communis	pycom10g01110
rosa_chinensis	RchiOBHm_Chr6g0276911
rosa_laevigata	RLG00000013355
rosa_multiflora	Rmu_sc0001425.1_g000018 Rmu_sc0003882.1_g000009
rosa_roxburghii	Rroxscaffold_7G00191800
rosa_rugosa	Rorug06G0104900
rosa_samantha	Rh6AG214800 Rh6BG219700 Rh6CG222300 Rh6DG212500
rosa_wichuraiana	Rw6G018790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	723, 1007
AarI	CACCTGC	1 cut(s)	2771
Acc36I	ACCTGC	1 cut(s)	2771
AccII	CGCG	1 cut(s)	106
AccIII	TCCGGA	1 cut(s)	1951
AciI	CCGC	8 cut(s)	98, 133, 647, 940, 2144, 2672, 2744, 2999
AcoI	YGGCCR	1 cut(s)	2032
AcsI	RAATTY	8 cut(s)	1710, 1947, 2466, 2474, 2529, 2547, 2714, 2863
AcuI	CTGAAG	5 cut(s)	73, 824, 908, 2730, 2955
AcvI	CACGTG	1 cut(s)	2738
AfaI	GTAC	4 cut(s)	131, 2298, 2351, 2444
AfiI	CCNNNNNNNGG	7 cut(s)	23, 139, 910, 1774, 2030, 2584, 2769
AgeI	ACCGGT	1 cut(s)	2401
AhdI	GACNNNNNGTC	1 cut(s)	2179
AjnI	CCWGG	1 cut(s)	759
AloI	GAACNNNNNNTCC	2 cut(s)	1780, 1812
Alw21I	GWGCWC	2 cut(s)	1098, 2743
Alw26I	GTCTC	6 cut(s)	1436, 1542, 2042, 2166, 2271, 2599
AlwNI	CAGNNNCTG	1 cut(s)	1897
Aor13HI	TCCGGA	1 cut(s)	1951
AoxI	GGCC	9 cut(s)	121, 385, 1062, 1452, 1859, 2032, 2251, 2930, 3127
ApaI	GGGCCC	1 cut(s)	1863
ApeKI	GCWGC	5 cut(s)	458, 473, 753, 1560, 2080
ApoI	RAATTY	8 cut(s)	1710, 1947, 2466, 2474, 2529, 2547, 2714, 2863
AsiGI	ACCGGT	1 cut(s)	2401
Asp700I	GAANNNNTTC	3 cut(s)	1341, 1524, 2391
AspLEI	GCGC	1 cut(s)	108
AspS9I	GGNCC	7 cut(s)	385, 937, 986, 1777, 1859, 1860, 2930
AsuC2I	CCSGG	1 cut(s)	389
AsuHPI	GGTGA	2 cut(s)	407, 2752
AvaII	GGWCC	3 cut(s)	937, 986, 1777
AxyI	CCTNAGG	1 cut(s)	1031
BaeGI	GKGCMC	1 cut(s)	1863
BaeI	ACNNNNGTAYC	2 cut(s)	2280, 2313
BanII	GRGCYC	3 cut(s)	1039, 1098, 1863
BauI	CACGAG	2 cut(s)	612, 2481
BbrPI	CACGTG	1 cut(s)	2738
BbsI	GAAGAC	4 cut(s)	627, 657, 791, 1512
Bbv12I	GWGCWC	2 cut(s)	1098, 2743
BbvI	GCAGC	5 cut(s)	470, 485, 740, 1572, 2067
BccI	CCATC	6 cut(s)	167, 1640, 1783, 1928, 2308, 2644
BceAI	ACGGC	2 cut(s)	2019, 2751
BciT130I	CCWGG	1 cut(s)	761
BciVI	GTATCC	1 cut(s)	94
BclI	TGATCA	1 cut(s)	1078
BcnI	CCSGG	1 cut(s)	389
BcoDI	GTCTC	6 cut(s)	1436, 1542, 2042, 2166, 2271, 2599
BfaI	CTAG	2 cut(s)	1502, 2345
BfmI	CTRYAG	2 cut(s)	2081, 2452
BfuAI	ACCTGC	1 cut(s)	2771
BfuI	GTATCC	1 cut(s)	94
BglII	AGATCT	1 cut(s)	1213
BisI	GCNGC	7 cut(s)	459, 474, 754, 1561, 2081, 2673, 2999
BlpI	GCTNAGC	1 cut(s)	1847
BlsI	GCNGC	7 cut(s)	460, 475, 755, 1562, 2082, 2674, 3000
Bme1390I	CCNGG	2 cut(s)	389, 761
Bme18I	GGWCC	3 cut(s)	937, 986, 1777
BmeRI	GACNNNNNGTC	1 cut(s)	2179
BmgT120I	GGNCC	7 cut(s)	385, 937, 986, 1777, 1859, 1860, 2930
BmiI	GGNNCC	3 cut(s)	28, 1778, 1861
BmrFI	CCNGG	2 cut(s)	389, 761
BmsI	GCATC	5 cut(s)	275, 1237, 1303, 1479, 2985
BoxI	GACNNNNGTC	1 cut(s)	1534
BpiI	GAAGAC	4 cut(s)	627, 657, 791, 1512
BplI	GAGNNNNNCTC	1 cut(s)	33
BpmI	CTGGAG	2 cut(s)	987, 2351
Bpu10I	CCTNAGC	1 cut(s)	413
Bpu1102I	GCTNAGC	1 cut(s)	1847
BpuEI	CTTGAG	1 cut(s)	2168
BpuMI	CCSGG	1 cut(s)	389
BsaAI	YACGTR	2 cut(s)	1130, 2738
BsaJI	CCNNGG	3 cut(s)	1065, 1678, 2496
BsaWI	WCCGGW	2 cut(s)	1951, 2401
BsaXI	ACNNNNNCTCC	4 cut(s)	1734, 1764, 2968, 2998
Bsc4I	CCNNNNNNNGG	7 cut(s)	23, 139, 910, 1774, 2030, 2584, 2769
Bse118I	RCCGGY	1 cut(s)	2401
Bse1I	ACTGG	4 cut(s)	1768, 1790, 2254, 2759
Bse21I	CCTNAGG	1 cut(s)	1031
BseAI	TCCGGA	1 cut(s)	1951
BseBI	CCWGG	1 cut(s)	761
BseDI	CCNNGG	3 cut(s)	1065, 1678, 2496
BseLI	CCNNNNNNNGG	7 cut(s)	23, 139, 910, 1774, 2030, 2584, 2769
BseMII	CTCAG	6 cut(s)	74, 513, 896, 1838, 1852, 2247
BseNI	ACTGG	4 cut(s)	1768, 1790, 2254, 2759
BseRI	GAGGAG	1 cut(s)	1755
BseSI	GKGCMC	1 cut(s)	1863
BseXI	GCAGC	5 cut(s)	470, 485, 740, 1572, 2067
BseYI	CCCAGC	1 cut(s)	3090
BsgI	GTGCAG	2 cut(s)	1849, 2718
Bsh1236I	CGCG	1 cut(s)	106
Bsh1285I	CGRYCG	1 cut(s)	1808
BshFI	GGCC	9 cut(s)	123, 387, 1064, 1454, 1861, 2034, 2253, 2932, 3129
BshTI	ACCGGT	1 cut(s)	2401
BsiEI	CGRYCG	1 cut(s)	1808
BsiHKAI	GWGCWC	2 cut(s)	1098, 2743
BsiSI	CCGG	3 cut(s)	388, 1952, 2402
BslFI	GGGAC	1 cut(s)	2843
BslI	CCNNNNNNNGG	7 cut(s)	23, 139, 910, 1774, 2030, 2584, 2769
BsmAI	GTCTC	6 cut(s)	1436, 1542, 2042, 2166, 2271, 2599
BsmBI	CGTCTC	1 cut(s)	2042
BsmFI	GGGAC	1 cut(s)	2843
BsnI	GGCC	9 cut(s)	123, 387, 1064, 1454, 1861, 2034, 2253, 2932, 3129
Bsp120I	GGGCCC	1 cut(s)	1859
Bsp1286I	GDGCHC	4 cut(s)	1039, 1098, 1863, 2743
Bsp13I	TCCGGA	1 cut(s)	1951
Bsp1407I	TGTACA	1 cut(s)	2442
Bsp1720I	GCTNAGC	1 cut(s)	1847
BspACI	CCGC	8 cut(s)	98, 133, 647, 940, 2144, 2672, 2744, 2999
BspANI	GGCC	9 cut(s)	123, 387, 1064, 1454, 1861, 2034, 2253, 2932, 3129
BspCNI	CTCAG	6 cut(s)	75, 512, 895, 1839, 1851, 2246
BspEI	TCCGGA	1 cut(s)	1951
BspFNI	CGCG	1 cut(s)	106
BspLI	GGNNCC	3 cut(s)	28, 1778, 1861
BspMAI	CTGCAG	1 cut(s)	2085
BspMI	ACCTGC	1 cut(s)	2771
BspQI	GCTCTTC	2 cut(s)	1103, 2781
BsrFI	RCCGGY	1 cut(s)	2401
BsrGI	TGTACA	1 cut(s)	2442
BsrI	ACTGG	4 cut(s)	1768, 1790, 2254, 2759
BssAI	RCCGGY	1 cut(s)	2401
BssECI	CCNNGG	3 cut(s)	1065, 1678, 2496
BssSI	CACGAG	2 cut(s)	612, 2481
BssT1I	CCWWGG	2 cut(s)	1065, 2496
Bst2BI	CACGAG	2 cut(s)	612, 2481
Bst2UI	CCWGG	1 cut(s)	761
Bst4CI	ACNGT	4 cut(s)	1569, 1679, 2442, 2968
Bst6I	CTCTTC	5 cut(s)	37, 43, 1103, 1639, 2781
BstAPI	GCANNNNNTGC	1 cut(s)	1897
BstAUI	TGTACA	1 cut(s)	2442
BstBAI	YACGTR	2 cut(s)	1130, 2738
BstC8I	GCNNGC	3 cut(s)	409, 2144, 2626
BstDSI	CCRYGG	1 cut(s)	1678
BstEII	GGTNACC	1 cut(s)	2635
BstFNI	CGCG	1 cut(s)	106
BstHHI	GCGC	1 cut(s)	108
BstMAI	GTCTC	6 cut(s)	1436, 1542, 2042, 2166, 2271, 2599
BstMCI	CGRYCG	1 cut(s)	1808
BstMWI	GCNNNNNNNGC	2 cut(s)	263, 1897
BstNI	CCWGG	1 cut(s)	761
BstNSI	RCATGY	3 cut(s)	1359, 1751, 2926
BstPAI	GACNNNNGTC	1 cut(s)	1534
BstPI	GGTNACC	1 cut(s)	2635
BstSCI	CCNGG	2 cut(s)	387, 759
BstSFI	CTRYAG	2 cut(s)	2081, 2452
BstSLI	GKGCMC	1 cut(s)	1863
BstSNI	TACGTA	1 cut(s)	1130
BstUI	CGCG	1 cut(s)	106
BstV1I	GCAGC	5 cut(s)	470, 485, 740, 1572, 2067
BstV2I	GAAGAC	4 cut(s)	627, 657, 791, 1512
BstX2I	RGATCY	4 cut(s)	358, 1213, 2128, 2197
BstYI	RGATCY	4 cut(s)	358, 1213, 2128, 2197
Bsu36I	CCTNAGG	1 cut(s)	1031
BsuI	GTATCC	1 cut(s)	94
BsuRI	GGCC	9 cut(s)	123, 387, 1064, 1454, 1861, 2034, 2253, 2932, 3129
BtgI	CCRYGG	1 cut(s)	1678
BtsIMutI	CAGTG	2 cut(s)	2208, 2247
BveI	ACCTGC	1 cut(s)	2771
Cac8I	GCNNGC	3 cut(s)	409, 2144, 2626
CaiI	CAGNNNCTG	1 cut(s)	1897
CfoI	GCGC	1 cut(s)	108
Cfr10I	RCCGGY	1 cut(s)	2401
Cfr13I	GGNCC	7 cut(s)	385, 937, 986, 1777, 1859, 1860, 2930
Csp6I	GTAC	4 cut(s)	130, 2297, 2350, 2443
CspAI	ACCGGT	1 cut(s)	2401
CspCI	CAANNNNNGTGG	2 cut(s)	1906, 1941
CviQI	GTAC	4 cut(s)	130, 2297, 2350, 2443
DraI	TTTAAA	4 cut(s)	579, 1155, 1366, 1717
DriI	GACNNNNNGTC	1 cut(s)	2179
EaeI	YGGCCR	1 cut(s)	2032
Eam1104I	CTCTTC	5 cut(s)	37, 43, 1103, 1639, 2781
Eam1105I	GACNNNNNGTC	1 cut(s)	2179
EarI	CTCTTC	5 cut(s)	37, 43, 1103, 1639, 2781
Ecl136II	GAGCTC	1 cut(s)	1096
Eco105I	TACGTA	1 cut(s)	1130
Eco130I	CCWWGG	2 cut(s)	1065, 2496
Eco147I	AGGCCT	1 cut(s)	1454
Eco24I	GRGCYC	3 cut(s)	1039, 1098, 1863
Eco32I	GATATC	1 cut(s)	549
Eco47I	GGWCC	3 cut(s)	937, 986, 1777
Eco53kI	GAGCTC	1 cut(s)	1096
Eco57I	CTGAAG	5 cut(s)	73, 824, 908, 2730, 2955
Eco72I	CACGTG	1 cut(s)	2738
Eco81I	CCTNAGG	1 cut(s)	1031
Eco91I	GGTNACC	1 cut(s)	2635
EcoICRI	GAGCTC	1 cut(s)	1096
EcoO109I	RGGNCCY	2 cut(s)	1777, 1860
EcoO65I	GGTNACC	1 cut(s)	2635
EcoRI	GAATTC	4 cut(s)	1947, 2474, 2714, 2863
EcoRII	CCWGG	1 cut(s)	759
EcoRV	GATATC	1 cut(s)	549
EcoT14I	CCWWGG	2 cut(s)	1065, 2496
EcoT22I	ATGCAT	4 cut(s)	268, 1252, 1318, 1494
EcoT38I	GRGCYC	3 cut(s)	1039, 1098, 1863
ErhI	CCWWGG	2 cut(s)	1065, 2496
Esp3I	CGTCTC	1 cut(s)	2042
FalI	AAGNNNNNCTT	8 cut(s)	266, 298, 416, 448, 612, 644, 1329, 1361
FaqI	GGGAC	1 cut(s)	2843
FauI	CCCGC	1 cut(s)	2151
FbaI	TGATCA	1 cut(s)	1078
Fnu4HI	GCNGC	7 cut(s)	459, 474, 754, 1561, 2081, 2673, 2999
FriOI	GRGCYC	3 cut(s)	1039, 1098, 1863
Fsp4HI	GCNGC	7 cut(s)	459, 474, 754, 1561, 2081, 2673, 2999
FspBI	CTAG	2 cut(s)	1502, 2345
GlaI	GCGC	1 cut(s)	107
GluI	GCNGC	7 cut(s)	459, 474, 754, 1561, 2081, 2673, 2999
GsaI	CCCAGC	1 cut(s)	3094
GsuI	CTGGAG	2 cut(s)	987, 2351
HaeIII	GGCC	9 cut(s)	123, 387, 1064, 1454, 1861, 2034, 2253, 2932, 3129
HapII	CCGG	3 cut(s)	388, 1952, 2402
HhaI	GCGC	1 cut(s)	108
Hin6I	GCGC	1 cut(s)	106
HinP1I	GCGC	1 cut(s)	106
HincII	GTYRAC	2 cut(s)	145, 2682
HindII	GTYRAC	2 cut(s)	145, 2682
HindIII	AAGCTT	4 cut(s)	1086, 1684, 1972, 2360
HpaII	CCGG	3 cut(s)	388, 1952, 2402
HphI	GGTGA	2 cut(s)	407, 2752
Hpy166II	GTNNAC	7 cut(s)	66, 145, 337, 937, 2682, 2890, 3100
Hpy8I	GTNNAC	7 cut(s)	66, 145, 337, 937, 2682, 2890, 3100
Hpy99I	CGWCG	3 cut(s)	367, 1834, 2772
HpyCH4III	ACNGT	4 cut(s)	1569, 1679, 2442, 2968
HpyCH4IV	ACGT	4 cut(s)	1129, 1168, 2326, 2737
HpyF10VI	GCNNNNNNNGC	2 cut(s)	263, 1897
HpySE526I	ACGT	4 cut(s)	1129, 1168, 2326, 2737
HspAI	GCGC	1 cut(s)	106
Kpn2I	TCCGGA	1 cut(s)	1951
Ksp22I	TGATCA	1 cut(s)	1078
LguI	GCTCTTC	2 cut(s)	1103, 2781
LmnI	GCTCC	3 cut(s)	9, 26, 2746
Lsp1109I	GCAGC	5 cut(s)	470, 485, 740, 1572, 2067
LweI	GCATC	5 cut(s)	275, 1237, 1303, 1479, 2985
MaeI	CTAG	2 cut(s)	1502, 2345
MaeII	ACGT	4 cut(s)	1129, 1168, 2326, 2737
MaeIII	GTNAC	7 cut(s)	856, 1370, 1409, 2223, 2365, 2635, 2962
MfeI	CAATTG	2 cut(s)	402, 2824
MflI	RGATCY	4 cut(s)	358, 1213, 2128, 2197
MhlI	GDGCHC	4 cut(s)	1039, 1098, 1863, 2743
MlyI	GAGTC	5 cut(s)	1544, 1864, 2601, 2677, 3105
MmeI	TCCRAC	7 cut(s)	345, 580, 1853, 2218, 2364, 2399, 2565
Mph1103I	ATGCAT	4 cut(s)	268, 1252, 1318, 1494
MroI	TCCGGA	1 cut(s)	1951
MroXI	GAANNNNTTC	3 cut(s)	1341, 1524, 2391
MspA1I	CMGCKG	1 cut(s)	886
MspI	CCGG	3 cut(s)	388, 1952, 2402
MspR9I	CCNGG	2 cut(s)	389, 761
MunI	CAATTG	2 cut(s)	402, 2824
MvaI	CCWGG	1 cut(s)	761
MvnI	CGCG	1 cut(s)	106
MwoI	GCNNNNNNNGC	2 cut(s)	263, 1897
NciI	CCSGG	1 cut(s)	389
NlaIV	GGNNCC	3 cut(s)	28, 1778, 1861
NmuCI	GTSAC	3 cut(s)	856, 1370, 1409
NsiI	ATGCAT	4 cut(s)	268, 1252, 1318, 1494
NspI	RCATGY	3 cut(s)	1359, 1751, 2926
PaqCI	CACCTGC	1 cut(s)	2771
PceI	AGGCCT	1 cut(s)	1454
PciSI	GCTCTTC	2 cut(s)	1103, 2781
PcsI	WCGNNNNNNNCGW	1 cut(s)	2046
PdmI	GAANNNNTTC	3 cut(s)	1341, 1524, 2391
PinAI	ACCGGT	1 cut(s)	2401
PkrI	GCNGC	7 cut(s)	460, 475, 755, 1562, 2082, 2674, 3000
Ple19I	CGATCG	1 cut(s)	1808
PleI	GAGTC	5 cut(s)	1543, 1864, 2601, 2677, 3104
PmaCI	CACGTG	1 cut(s)	2738
PmlI	CACGTG	1 cut(s)	2738
PpsI	GAGTC	5 cut(s)	1543, 1864, 2601, 2677, 3104
Ppu21I	YACGTR	2 cut(s)	1130, 2738
PpuMI	RGGWCCY	1 cut(s)	1777
PshAI	GACNNNNGTC	1 cut(s)	1534
PsiI	TTATAA	2 cut(s)	723, 1007
Psp124BI	GAGCTC	1 cut(s)	1098
Psp5II	RGGWCCY	1 cut(s)	1777
Psp6I	CCWGG	1 cut(s)	759
PspCI	CACGTG	1 cut(s)	2738
PspEI	GGTNACC	1 cut(s)	2635
PspFI	CCCAGC	1 cut(s)	3090
PspGI	CCWGG	1 cut(s)	759
PspN4I	GGNNCC	3 cut(s)	28, 1778, 1861
PspOMI	GGGCCC	1 cut(s)	1859
PspPI	GGNCC	7 cut(s)	385, 937, 986, 1777, 1859, 1860, 2930
PspPPI	RGGWCCY	1 cut(s)	1777
PstI	CTGCAG	1 cut(s)	2085
PstNI	CAGNNNCTG	1 cut(s)	1897
PsuI	RGATCY	4 cut(s)	358, 1213, 2128, 2197
PvuI	CGATCG	1 cut(s)	1808
PvuII	CAGCTG	1 cut(s)	886
RsaI	GTAC	4 cut(s)	131, 2298, 2351, 2444
RsaNI	GTAC	4 cut(s)	130, 2297, 2350, 2443
SacI	GAGCTC	1 cut(s)	1098
SapI	GCTCTTC	2 cut(s)	1103, 2781
SatI	GCNGC	7 cut(s)	459, 474, 754, 1561, 2081, 2673, 2999
Sau96I	GGNCC	7 cut(s)	385, 937, 986, 1777, 1859, 1860, 2930
SchI	GAGTC	5 cut(s)	1544, 1864, 2601, 2677, 3105
ScrFI	CCNGG	2 cut(s)	389, 761
SduI	GDGCHC	4 cut(s)	1039, 1098, 1863, 2743
SfaNI	GCATC	5 cut(s)	275, 1237, 1303, 1479, 2985
SfcI	CTRYAG	2 cut(s)	2081, 2452
SinI	GGWCC	3 cut(s)	937, 986, 1777
SmlI	CTYRAG	1 cut(s)	2147
SmoI	CTYRAG	1 cut(s)	2147
SnaBI	TACGTA	1 cut(s)	1130
SseBI	AGGCCT	1 cut(s)	1454
SsiI	CCGC	8 cut(s)	98, 133, 647, 940, 2144, 2672, 2744, 2999
SspI	AATATT	1 cut(s)	1483
SspMI	CTAG	2 cut(s)	1502, 2345
SstI	GAGCTC	1 cut(s)	1098
StuI	AGGCCT	1 cut(s)	1454
StyD4I	CCNGG	2 cut(s)	387, 759
StyI	CCWWGG	2 cut(s)	1065, 2496
TaaI	ACNGT	4 cut(s)	1569, 1679, 2442, 2968
TaiI	ACGT	4 cut(s)	1132, 1171, 2329, 2740
TaqI	TCGA	8 cut(s)	213, 1528, 1722, 2040, 2303, 2861, 3016, 3108
TatI	WGTACW	1 cut(s)	2442
TauI	GCSGC	2 cut(s)	2675, 3001
TscAI	CASTG	2 cut(s)	2215, 2254
TseFI	GTSAC	3 cut(s)	856, 1370, 1409
TseI	GCWGC	5 cut(s)	458, 473, 753, 1560, 2080
Tsp45I	GTSAC	3 cut(s)	856, 1370, 1409
TspGWI	ACGGA	5 cut(s)	229, 351, 1176, 1631, 2567
TspRI	CASTG	2 cut(s)	2215, 2254
VpaK11BI	GGWCC	3 cut(s)	937, 986, 1777
XapI	RAATTY	8 cut(s)	1710, 1947, 2466, 2474, 2529, 2547, 2714, 2863
XbaI	TCTAGA	1 cut(s)	1501
XceI	RCATGY	3 cut(s)	1359, 1751, 2926
XcmI	CCANNNNNNNNNTGG	2 cut(s)	328, 2647
XmnI	GAANNNNTTC	3 cut(s)	1341, 1524, 2391
XspI	CTAG	2 cut(s)	1502, 2345
Zsp2I	ATGCAT	4 cut(s)	268, 1252, 1318, 1494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.