Rh1DG234100

Peroxisome biogenesis protein 16-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0004742
Physical Location & Seq
Reverse (-)
44675203 .. 44677880
2678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG234100.1

Sequence Viewer

Length: 1110 bp
ATGGAGGCTTATAAGAAATGGGTCAGGGAGAACAAGGACTATGTTCACTCCCTGGAGTCTCTCGCCAATGGATTCACTTGGCTTCTACCTGAACGGTTTTCTCAATCAGAGATAGGACCAGAAGCAGTAACTACTATCTTGGGAATATTAACTGCTGTCAATGAACATATAATCGACACAGCTTCTCCTCGATTACATGCTGGCCATGCTGAGCGTAATTCTTTTCCTTATCCATTGTGTATATCTGCACTAAAGGACTTGGAAACGTTGGTTGAAGTTGTGGCTCAACAGTACTTTGGTGATGACAAAAAATGGAATTACATTGCTATTATGGAGGCTAGCAAGGTGCTAGTTAGGTTAGCTTTGTTCCGGAACAGTGGATATAAGATGCTTCTCCATGGAGGAGAGACACCAAATGATGAAAAAAATTTGGATGCTTCAACTCCGCAGCGTAGAAGTGGTGGTTTTATGAAGCCTGGCGAGCAGCATGGGAATGGTCACTTGAGAAATAATCATGCACAAGATCCATGGAATTTAGAAGGAAGAGCACTATCTGCATTGAATAGGTTTGGAGAAAAGGCTCGGACTGTTTCAGATCCAGTATGGTTGCGTAGGGTTCAACATCAGCATGCCATTATGGAGCCTCCAACTCCAGTGGTTGAGAGGTCGACTCTATCCACAATATTGTCCGAAAAGGGTCTCTATGGGGCTTTGTATGTGGCTGGGGAAGTGCTATTTATAACAAGACCACTTATCTATGTTTTATTTATTCGGAAATATGGAGCTCGCTCCTGGATTCCTTGGTTTCTTTCGTTGGCTGTGGACTTCACTGGAATGGGCATTCTTTCTCGAATTACTTCGTCCACAGGTAGTACAAAAGAGCAGCAGTTTCATCTTTCTGTCCCTGAAAAGAATGAGGTTAAAAGACGAAAACTGTTATGGGCACTTTACCTCATGAGAGATCCATTTTTCAGCAAGTATACCAGGCGAAGACTTGAAGGCACTGAGAATATGTTGGAACATATACCAGTTGTTGGATTTCTTTCAGCAAAACTTGTTGAGCTTATTTCAGGAGCACAGACAAGGTATACTTACATGTCGGGATCATAA

Protein Analysis

369

Amino Acids

41.94

Weight (kDa)

9.28

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pex16 PF08610 2 - 360 2.3e-91 Peroxisomal membrane protein (Pex16)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 12, 740
AccB7I CCANNNNNTGG 1 cut(s) 1034
AccI GTMKAC 3 cut(s) 668, 980, 1088
AccIII TCCGGA 1 cut(s) 369
AciI CCGC 1 cut(s) 446
AclI AACGTT 1 cut(s) 266
AclWI GGATC 3 cut(s) 518, 590, 956
AcoI YGGCCR 1 cut(s) 202
AcsI RAATTY 2 cut(s) 427, 532
AfaI GTAC 2 cut(s) 293, 874
AfiI CCNNNNNNNGG 1 cut(s) 1034
AflIII ACRYGT 1 cut(s) 1095
AgsI TTSAA 5 cut(s) 275, 441, 562, 620, 998
AjnI CCWGG 4 cut(s) 51, 475, 791, 983
AluBI AGCT 4 cut(s) 182, 362, 785, 1063
AluI AGCT 4 cut(s) 182, 362, 785, 1063
Alw21I GWGCWC 3 cut(s) 550, 787, 1078
Alw26I GTCTC 3 cut(s) 63, 401, 704
AlwI GGATC 3 cut(s) 518, 590, 956
Aor13HI TCCGGA 1 cut(s) 369
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 3 cut(s) 448, 484, 883
ApoI RAATTY 2 cut(s) 427, 532
Asp700I GAANNNNTTC 1 cut(s) 856
AspS9I GGNCC 1 cut(s) 116
AsuHPI GGTGA 1 cut(s) 311
AsuNHI GCTAGC 1 cut(s) 338
AvaII GGWCC 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 946
BalI TGGCCA 1 cut(s) 204
BanII GRGCYC 1 cut(s) 787
BbsI GAAGAC 1 cut(s) 997
Bbv12I GWGCWC 3 cut(s) 550, 787, 1078
BbvI GCAGC 3 cut(s) 460, 496, 895
BciT130I CCWGG 4 cut(s) 53, 477, 793, 985
BcoDI GTCTC 3 cut(s) 63, 401, 704
BfaI CTAG 2 cut(s) 339, 350
BisI GCNGC 3 cut(s) 449, 485, 884
BlpI GCTNAGC 1 cut(s) 210
BlsI GCNGC 3 cut(s) 450, 486, 885
BmcAI AGTACT 1 cut(s) 293
Bme1390I CCNGG 4 cut(s) 53, 477, 793, 985
Bme18I GGWCC 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 642
BmrFI CCNGG 4 cut(s) 53, 477, 793, 985
BmsI GCATC 2 cut(s) 378, 424
BmtI GCTAGC 1 cut(s) 342
BpiI GAAGAC 1 cut(s) 997
BplI GAGNNNNNCTC 2 cut(s) 655, 687
BpmI CTGGAG 2 cut(s) 74, 636
Bpu1102I GCTNAGC 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 523
BsaI GGTCTC 1 cut(s) 704
BsaJI CCNNGG 4 cut(s) 51, 397, 527, 800
BsaWI WCCGGW 1 cut(s) 369
BsaXI ACNNNNNCTCC 2 cut(s) 169, 199
Bsc4I CCNNNNNNNGG 1 cut(s) 1034
Bse1I ACTGG 4 cut(s) 599, 653, 835, 1028
Bse3DI GCAATG 1 cut(s) 321
BseAI TCCGGA 1 cut(s) 369
BseBI CCWGG 4 cut(s) 53, 477, 793, 985
BseDI CCNNGG 4 cut(s) 51, 397, 527, 800
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 1 cut(s) 1034
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 2 cut(s) 201, 996
BseNI ACTGG 4 cut(s) 599, 653, 835, 1028
BseRI GAGGAG 2 cut(s) 177, 417
BseSI GKGCMC 1 cut(s) 946
BseXI GCAGC 3 cut(s) 460, 496, 895
BseYI CCCAGC 1 cut(s) 722
BsgI GTGCAG 1 cut(s) 231
BshFI GGCC 1 cut(s) 204
BsiHKAI GWGCWC 3 cut(s) 550, 787, 1078
BsiSI CCGG 1 cut(s) 370
BslFI GGGAC 1 cut(s) 887
BslI CCNNNNNNNGG 1 cut(s) 1034
BsmAI GTCTC 3 cut(s) 63, 401, 704
BsmFI GGGAC 1 cut(s) 887
BsmI GAATGC 1 cut(s) 840
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 704
Bsp1286I GDGCHC 4 cut(s) 550, 787, 946, 1078
Bsp13I TCCGGA 1 cut(s) 369
Bsp143I GATC 4 cut(s) 523, 595, 961, 1103
Bsp1720I GCTNAGC 1 cut(s) 210
Bsp19I CCATGG 2 cut(s) 397, 527
BspACI CCGC 1 cut(s) 446
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 2 cut(s) 202, 997
BspEI TCCGGA 1 cut(s) 369
BspHI TCATGA 1 cut(s) 954
BspLI GGNNCC 1 cut(s) 642
BspOI GCTAGC 1 cut(s) 342
BspPI GGATC 3 cut(s) 518, 590, 956
BspQI GCTCTTC 1 cut(s) 538
BspTNI GGTCTC 1 cut(s) 704
BsrDI GCAATG 1 cut(s) 321
BsrI ACTGG 4 cut(s) 599, 653, 835, 1028
BssECI CCNNGG 4 cut(s) 51, 397, 527, 800
BssMI GATC 4 cut(s) 523, 595, 961, 1103
BssNAI GTATAC 2 cut(s) 981, 1089
BssT1I CCWWGG 3 cut(s) 397, 527, 800
Bst1107I GTATAC 2 cut(s) 981, 1089
Bst2UI CCWGG 4 cut(s) 53, 477, 793, 985
Bst4CI ACNGT 5 cut(s) 96, 291, 377, 589, 936
Bst6I CTCTTC 1 cut(s) 538
BstAPI GCANNNNNTGC 1 cut(s) 554
BstC8I GCNNGC 5 cut(s) 202, 340, 482, 630, 787
BstDEI CTNAG 2 cut(s) 210, 1005
BstDSI CCRYGG 2 cut(s) 397, 527
BstF5I GGATG 1 cut(s) 439
BstKTI GATC 4 cut(s) 526, 598, 964, 1106
BstMAI GTCTC 3 cut(s) 63, 401, 704
BstMBI GATC 4 cut(s) 523, 595, 961, 1103
BstMWI GCNNNNNNNGC 3 cut(s) 206, 481, 554
BstNI CCWGG 4 cut(s) 53, 477, 793, 985
BstNSI RCATGY 3 cut(s) 200, 632, 1099
BstSCI CCNGG 4 cut(s) 51, 475, 791, 983
BstSLI GKGCMC 1 cut(s) 946
BstV1I GCAGC 3 cut(s) 460, 496, 895
BstV2I GAAGAC 1 cut(s) 997
BstX2I RGATCY 3 cut(s) 523, 595, 961
BstYI RGATCY 3 cut(s) 523, 595, 961
BstZ17I GTATAC 2 cut(s) 981, 1089
BsuRI GGCC 1 cut(s) 204
BtgI CCRYGG 2 cut(s) 397, 527
BtsCI GGATG 1 cut(s) 439
BtsIMutI CAGTG 4 cut(s) 382, 660, 828, 1002
Cac8I GCNNGC 5 cut(s) 202, 340, 482, 630, 787
CciI TCATGA 1 cut(s) 954
Cfr13I GGNCC 1 cut(s) 116
Csp6I GTAC 2 cut(s) 292, 873
CspCI CAANNNNNGTGG 2 cut(s) 636, 671
CviAII CATG 9 cut(s) 197, 206, 398, 488, 515, 528, 629, 955, 1096
CviQI GTAC 2 cut(s) 292, 873
DdeI CTNAG 2 cut(s) 210, 1005
DpnI GATC 4 cut(s) 525, 597, 963, 1105
DpnII GATC 4 cut(s) 523, 595, 961, 1103
EaeI YGGCCR 1 cut(s) 202
Eam1104I CTCTTC 1 cut(s) 538
EarI CTCTTC 1 cut(s) 538
Ecl136II GAGCTC 1 cut(s) 785
Eco130I CCWWGG 3 cut(s) 397, 527, 800
Eco24I GRGCYC 1 cut(s) 787
Eco31I GGTCTC 1 cut(s) 704
Eco47I GGWCC 1 cut(s) 116
Eco53kI GAGCTC 1 cut(s) 785
EcoICRI GAGCTC 1 cut(s) 785
EcoRII CCWGG 4 cut(s) 51, 475, 791, 983
EcoT14I CCWWGG 3 cut(s) 397, 527, 800
EcoT38I GRGCYC 1 cut(s) 787
ErhI CCWWGG 3 cut(s) 397, 527, 800
FaeI CATG 9 cut(s) 200, 209, 401, 491, 518, 531, 632, 958, 1099
FalI AAGNNNNNCTT 2 cut(s) 1075, 1107
FaqI GGGAC 1 cut(s) 887
FatI CATG 9 cut(s) 196, 205, 397, 487, 514, 527, 628, 954, 1095
FblI GTMKAC 3 cut(s) 668, 980, 1088
Fnu4HI GCNGC 3 cut(s) 449, 485, 884
FokI GGATG 1 cut(s) 446
FriOI GRGCYC 1 cut(s) 787
Fsp4HI GCNGC 3 cut(s) 449, 485, 884
FspBI CTAG 2 cut(s) 339, 350
GluI GCNGC 3 cut(s) 449, 485, 884
GsaI CCCAGC 1 cut(s) 726
GsuI CTGGAG 2 cut(s) 74, 636
HaeIII GGCC 1 cut(s) 204
HapII CCGG 1 cut(s) 370
Hin1II CATG 9 cut(s) 200, 209, 401, 491, 518, 531, 632, 958, 1099
HincII GTYRAC 1 cut(s) 669
HindII GTYRAC 1 cut(s) 669
HinfI GANTC 4 cut(s) 56, 72, 670, 796
HpaII CCGG 1 cut(s) 370
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 6 cut(s) 46, 669, 823, 864, 981, 1089
Hpy188I TCNGA 5 cut(s) 109, 585, 595, 691, 774
Hpy188III TCNNGA 5 cut(s) 370, 849, 955, 1071, 1101
Hpy8I GTNNAC 6 cut(s) 46, 669, 823, 864, 981, 1089
HpyAV CCTTC 2 cut(s) 533, 992
HpyCH4III ACNGT 5 cut(s) 96, 291, 377, 589, 936
HpyCH4IV ACGT 1 cut(s) 266
HpyCH4V TGCA 3 cut(s) 248, 518, 557
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 481, 554
HpyF3I CTNAG 2 cut(s) 210, 1005
HpySE526I ACGT 1 cut(s) 266
Hsp92II CATG 9 cut(s) 200, 209, 401, 491, 518, 531, 632, 958, 1099
Kpn2I TCCGGA 1 cut(s) 369
Kzo9I GATC 4 cut(s) 523, 595, 961, 1103
LguI GCTCTTC 1 cut(s) 538
LmnI GCTCC 4 cut(s) 640, 782, 794, 1073
Lsp1109I GCAGC 3 cut(s) 460, 496, 895
LweI GCATC 2 cut(s) 378, 424
MaeI CTAG 2 cut(s) 339, 350
MaeII ACGT 1 cut(s) 266
MaeIII GTNAC 2 cut(s) 127, 497
MalI GATC 4 cut(s) 525, 597, 963, 1105
MboI GATC 4 cut(s) 523, 595, 961, 1103
MboII GAAGA 2 cut(s) 555, 1002
MflI RGATCY 3 cut(s) 523, 595, 961
MhlI GDGCHC 4 cut(s) 550, 787, 946, 1078
MlsI TGGCCA 1 cut(s) 204
MluCI AATT 5 cut(s) 217, 316, 427, 532, 852
MluNI TGGCCA 1 cut(s) 204
MlyI GAGTC 2 cut(s) 65, 664
MmeI TCCRAC 3 cut(s) 671, 996, 1015
MnlI CCTC 7 cut(s) 198, 328, 395, 654, 657, 910, 962
Mox20I TGGCCA 1 cut(s) 204
MroI TCCGGA 1 cut(s) 369
MroXI GAANNNNTTC 1 cut(s) 856
MscI TGGCCA 1 cut(s) 204
MseI TTAA 2 cut(s) 149, 921
MslI CAYNNNNRTG 3 cut(s) 492, 627, 833
Msp20I TGGCCA 1 cut(s) 204
MspI CCGG 1 cut(s) 370
MspR9I CCNGG 4 cut(s) 53, 477, 793, 985
Mva1269I GAATGC 1 cut(s) 840
MvaI CCWGG 4 cut(s) 53, 477, 793, 985
MwoI GCNNNNNNNGC 3 cut(s) 206, 481, 554
NcoI CCATGG 2 cut(s) 397, 527
NdeII GATC 4 cut(s) 523, 595, 961, 1103
NheI GCTAGC 1 cut(s) 338
NlaIII CATG 9 cut(s) 200, 209, 401, 491, 518, 531, 632, 958, 1099
NlaIV GGNNCC 1 cut(s) 642
NmuCI GTSAC 1 cut(s) 497
NspI RCATGY 3 cut(s) 200, 632, 1099
PaeI GCATGC 1 cut(s) 632
PagI TCATGA 1 cut(s) 954
PciI ACATGT 1 cut(s) 1095
PciSI GCTCTTC 1 cut(s) 538
PctI GAATGC 1 cut(s) 840
PdmI GAANNNNTTC 1 cut(s) 856
PfeI GAWTC 2 cut(s) 72, 796
PflMI CCANNNNNTGG 1 cut(s) 1034
PfoI TCCNGGA 1 cut(s) 791
PkrI GCNGC 3 cut(s) 450, 486, 885
PleI GAGTC 2 cut(s) 64, 664
PpsI GAGTC 2 cut(s) 64, 664
PscI ACATGT 1 cut(s) 1095
PsiI TTATAA 2 cut(s) 12, 740
Psp124BI GAGCTC 1 cut(s) 787
Psp1406I AACGTT 1 cut(s) 266
Psp6I CCWGG 4 cut(s) 51, 475, 791, 983
PspFI CCCAGC 1 cut(s) 722
PspGI CCWGG 4 cut(s) 51, 475, 791, 983
PspN4I GGNNCC 1 cut(s) 642
PspPI GGNCC 1 cut(s) 116
PsuI RGATCY 3 cut(s) 523, 595, 961
RsaI GTAC 2 cut(s) 293, 874
RsaNI GTAC 2 cut(s) 292, 873
RseI CAYNNNNRTG 3 cut(s) 492, 627, 833
SacI GAGCTC 1 cut(s) 787
SalI GTCGAC 1 cut(s) 667
SapI GCTCTTC 1 cut(s) 538
SaqAI TTAA 2 cut(s) 149, 921
SatI GCNGC 3 cut(s) 449, 485, 884
Sau3AI GATC 4 cut(s) 523, 595, 961, 1103
Sau96I GGNCC 1 cut(s) 116
ScaI AGTACT 1 cut(s) 293
SchI GAGTC 2 cut(s) 65, 664
ScrFI CCNGG 4 cut(s) 53, 477, 793, 985
SduI GDGCHC 4 cut(s) 550, 787, 946, 1078
SfaNI GCATC 2 cut(s) 378, 424
SinI GGWCC 1 cut(s) 116
SmiMI CAYNNNNRTG 3 cut(s) 492, 627, 833
SmlI CTYRAG 1 cut(s) 502
SmoI CTYRAG 1 cut(s) 502
SphI GCATGC 1 cut(s) 632
Sse9I AATT 5 cut(s) 217, 316, 427, 532, 852
SsiI CCGC 1 cut(s) 446
SspI AATATT 2 cut(s) 147, 684
SspMI CTAG 2 cut(s) 339, 350
SstI GAGCTC 1 cut(s) 787
StyD4I CCNGG 4 cut(s) 51, 475, 791, 983
StyI CCWWGG 3 cut(s) 397, 527, 800
TaaI ACNGT 5 cut(s) 96, 291, 377, 589, 936
TaiI ACGT 1 cut(s) 269
TaqI TCGA 4 cut(s) 174, 190, 668, 850
TasI AATT 5 cut(s) 217, 316, 427, 532, 852
TatI WGTACW 2 cut(s) 291, 872
TfiI GAWTC 2 cut(s) 72, 796
Tru1I TTAA 2 cut(s) 149, 921
Tru9I TTAA 2 cut(s) 149, 921
TscAI CASTG 4 cut(s) 382, 660, 835, 1009
TseFI GTSAC 1 cut(s) 497
TseI GCWGC 3 cut(s) 448, 484, 883
Tsp45I GTSAC 1 cut(s) 497
TspDTI ATGAA 4 cut(s) 177, 435, 485, 881
TspRI CASTG 4 cut(s) 382, 660, 835, 1009
Van91I CCANNNNNTGG 1 cut(s) 1034
VpaK11BI GGWCC 1 cut(s) 116
XapI RAATTY 2 cut(s) 427, 532
XceI RCATGY 3 cut(s) 200, 632, 1099
XmiI GTMKAC 3 cut(s) 668, 980, 1088
XmnI GAANNNNTTC 1 cut(s) 856
XspI CTAG 2 cut(s) 339, 350
ZrmI AGTACT 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.