Rh2AG051300

Chloroplast processing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010175
Physical Location & Seq
Reverse (-)
4052246 .. 4057400
5155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG051300.1

Sequence Viewer

Length: 780 bp
ATGCTCTCTCTACAGTTCTTCCCTCCATTACCTTCCCTCCAAAACCCTAATCCCGACCACACCCACTTCTCCATTCCCATCAAAAACCCCAATTTCACATCCCTCAATCTCCGCCCACTTTCCAAAACCCCCAATTCCAAGCTCTCAATCAAAACCCACTTCAGAAAACTCGTCTGCAATGCGTTGAAGGACTCCGGTAAAGAAAAGAAGGTGTCTTTGGACAGTGGCGGCGGCGGAGACGGCGGAGAAGGCGGCGGTGGTGACGGTGGCGATGATGAGCAAGGGGAGAAGAAAAGTGGGCCCCTGCCGGAATGGTTGAATATAACAACTGATGATGCCAAGACGGTGTTTGCGGCAATTGCAGTATCGCTTGCGTTTCGGTCTTTCATAGCTGAGCCGAGGTTTATTCCTTCCTTGTCAATGTATCCTACTTTGGATGTTGGGGATAGAATTGTAGCTGAAAAGGTTTCATATTACTTCAGAAAGCCATGCGCCAATGATATAGTTATCTTTAAAAGTCCACCGGTCCTTCAGGAAGTAGGATATACTGACCTTGATGTCTTCATTAAACGAATAGTGGCAAAGGAAGGTGATACCGTAGAGGTCCGCAATGGGAAGCTCATAGTTAATGGGGTGGAGAGAAATGAAAAATTCATCCTTGAACCACCTTCTTATAATATGACGCCTATTGTAAGAATCACTTTTAACAGTTTACCTTCTTTTTCTTCCTTTATACCCTTCTTTTCTTTACTATTATCCGGAAACTTGGTAAGGTTGTAA

Protein Analysis

259

Amino Acids

28.46

Weight (kDa)

8.43

Isoelectric Point (pI)

30.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S26 PF10502 113 - 224 1.4e-24 Signal peptidase, peptidase S26
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 675
AasI GACNNNNNNGTC 1 cut(s) 557
AccIII TCCGGA 1 cut(s) 758
AciI CCGC 9 cut(s) 112, 228, 231, 234, 243, 252, 255, 353, 607
AcsI RAATTY 1 cut(s) 650
AcuI CTGAAG 3 cut(s) 145, 463, 515
AcyI GRCGYC 1 cut(s) 683
AgeI ACCGGT 1 cut(s) 523
AgsI TTSAA 3 cut(s) 187, 319, 662
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 4 cut(s) 142, 392, 458, 619
AluI AGCT 4 cut(s) 142, 392, 458, 619
Alw26I GTCTC 1 cut(s) 231
Aor13HI TCCGGA 1 cut(s) 758
AoxI GGCC 1 cut(s) 299
ApaI GGGCCC 1 cut(s) 303
ApoI RAATTY 1 cut(s) 650
AsiGI ACCGGT 1 cut(s) 523
AspLEI GCGC 1 cut(s) 494
AspS9I GGNCC 4 cut(s) 299, 300, 526, 604
AsuHPI GGTGA 2 cut(s) 272, 602
AvaII GGWCC 2 cut(s) 526, 604
BaeGI GKGCMC 1 cut(s) 303
BanII GRGCYC 1 cut(s) 303
BbsI GAAGAC 1 cut(s) 553
BccI CCATC 1 cut(s) 86
BceAI ACGGC 1 cut(s) 256
BciVI GTATCC 1 cut(s) 435
BcoDI GTCTC 1 cut(s) 231
BfmI CTRYAG 1 cut(s) 11
BfuI GTATCC 1 cut(s) 435
BisI GCNGC 4 cut(s) 229, 232, 253, 354
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 4 cut(s) 230, 233, 254, 355
Bme18I GGWCC 2 cut(s) 526, 604
BmgT120I GGNCC 4 cut(s) 299, 300, 526, 604
BmiI GGNNCC 2 cut(s) 301, 302
BmsI GCATC 1 cut(s) 325
BpiI GAAGAC 1 cut(s) 553
Bpu1102I GCTNAGC 1 cut(s) 393
BsaHI GRCGYC 1 cut(s) 683
BsaJI CCNNGG 1 cut(s) 398
BsaWI WCCGGW 3 cut(s) 194, 523, 758
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bse118I RCCGGY 1 cut(s) 523
Bse3DI GCAATG 2 cut(s) 184, 616
BseAI TCCGGA 1 cut(s) 758
BseDI CCNNGG 1 cut(s) 398
BseGI GGATG 3 cut(s) 98, 442, 654
BseMI GCAATG 2 cut(s) 184, 616
BseMII CTCAG 1 cut(s) 384
BseSI GKGCMC 1 cut(s) 303
BshFI GGCC 1 cut(s) 301
BshTI ACCGGT 1 cut(s) 523
BsiSI CCGG 4 cut(s) 195, 308, 524, 759
BsmAI GTCTC 1 cut(s) 231
BsmBI CGTCTC 1 cut(s) 231
BsnI GGCC 1 cut(s) 301
Bsp120I GGGCCC 1 cut(s) 299
Bsp1286I GDGCHC 1 cut(s) 303
Bsp13I TCCGGA 1 cut(s) 758
Bsp1720I GCTNAGC 1 cut(s) 393
BspACI CCGC 9 cut(s) 112, 228, 231, 234, 243, 252, 255, 353, 607
BspANI GGCC 1 cut(s) 301
BspCNI CTCAG 1 cut(s) 385
BspEI TCCGGA 1 cut(s) 758
BspLI GGNNCC 2 cut(s) 301, 302
BsrDI GCAATG 2 cut(s) 184, 616
BsrFI RCCGGY 1 cut(s) 523
BssAI RCCGGY 1 cut(s) 523
BssECI CCNNGG 1 cut(s) 398
BssNI GRCGYC 1 cut(s) 683
Bst4CI ACNGT 6 cut(s) 15, 224, 266, 346, 598, 710
BstACI GRCGYC 1 cut(s) 683
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 1 cut(s) 393
BstF5I GGATG 3 cut(s) 98, 442, 654
BstHHI GCGC 1 cut(s) 494
BstMAI GTCTC 1 cut(s) 231
BstMWI GCNNNNNNNGC 3 cut(s) 240, 249, 359
BstSFI CTRYAG 1 cut(s) 11
BstSLI GKGCMC 1 cut(s) 303
BstV2I GAAGAC 1 cut(s) 553
BsuI GTATCC 1 cut(s) 435
BsuRI GGCC 1 cut(s) 301
BtgZI GCGATG 1 cut(s) 285
BtsCI GGATG 3 cut(s) 98, 442, 654
BtsIMutI CAGTG 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 372
CfoI GCGC 1 cut(s) 494
Cfr10I RCCGGY 1 cut(s) 523
Cfr13I GGNCC 4 cut(s) 299, 300, 526, 604
CseI GACGC 1 cut(s) 691
CspAI ACCGGT 1 cut(s) 523
CviAII CATG 1 cut(s) 489
CviJI RGCY 7 cut(s) 142, 301, 392, 397, 458, 487, 619
CviKI_1 RGCY 7 cut(s) 142, 301, 392, 397, 458, 487, 619
DdeI CTNAG 1 cut(s) 393
DraI TTTAAA 1 cut(s) 514
DrdI GACNNNNNNGTC 1 cut(s) 557
DseDI GACNNNNNNGTC 1 cut(s) 557
EciI GGCGGA 3 cut(s) 101, 249, 258
Eco24I GRGCYC 1 cut(s) 303
Eco47I GGWCC 2 cut(s) 526, 604
Eco57I CTGAAG 3 cut(s) 145, 463, 515
EcoO109I RGGNCCY 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 303
Esp3I CGTCTC 1 cut(s) 231
FaeI CATG 1 cut(s) 492
FalI AAGNNNNNCTT 2 cut(s) 685, 717
FatI CATG 1 cut(s) 488
Fnu4HI GCNGC 4 cut(s) 229, 232, 253, 354
FokI GGATG 3 cut(s) 85, 449, 641
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 4 cut(s) 229, 232, 253, 354
GlaI GCGC 1 cut(s) 493
GluI GCNGC 4 cut(s) 229, 232, 253, 354
HaeIII GGCC 1 cut(s) 301
HapII CCGG 4 cut(s) 195, 308, 524, 759
HgaI GACGC 1 cut(s) 691
HhaI GCGC 1 cut(s) 494
Hin1I GRCGYC 1 cut(s) 683
Hin1II CATG 1 cut(s) 492
Hin6I GCGC 1 cut(s) 492
HinP1I GCGC 1 cut(s) 492
HinfI GANTC 2 cut(s) 191, 696
HpaII CCGG 4 cut(s) 195, 308, 524, 759
HphI GGTGA 2 cut(s) 272, 602
Hpy166II GTNNAC 2 cut(s) 521, 713
Hpy188I TCNGA 2 cut(s) 164, 482
Hpy188III TCNNGA 3 cut(s) 53, 533, 759
Hpy8I GTNNAC 2 cut(s) 521, 713
HpyCH4III ACNGT 6 cut(s) 15, 224, 266, 346, 598, 710
HpyCH4V TGCA 2 cut(s) 177, 362
HpyF10VI GCNNNNNNNGC 3 cut(s) 240, 249, 359
HpyF3I CTNAG 1 cut(s) 393
Hsp92I GRCGYC 1 cut(s) 683
Hsp92II CATG 1 cut(s) 492
HspAI GCGC 1 cut(s) 492
Kpn2I TCCGGA 1 cut(s) 758
LpnPI CCDG 6 cut(s) 208, 317, 321, 518, 537, 772
LweI GCATC 1 cut(s) 325
MaeIII GTNAC 1 cut(s) 260
MboII GAAGA 4 cut(s) 10, 301, 553, 717
MfeI CAATTG 1 cut(s) 357
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 5 cut(s) 91, 133, 357, 450, 650
MlyI GAGTC 1 cut(s) 185
MnlI CCTC 5 cut(s) 33, 47, 113, 393, 595
MroI TCCGGA 1 cut(s) 758
MseI TTAA 4 cut(s) 513, 567, 627, 705
MspI CCGG 4 cut(s) 195, 308, 524, 759
MunI CAATTG 1 cut(s) 357
MwoI GCNNNNNNNGC 3 cut(s) 240, 249, 359
NlaIII CATG 1 cut(s) 492
NlaIV GGNNCC 2 cut(s) 301, 302
NmeAIII GCCGAG 1 cut(s) 423
NmuCI GTSAC 1 cut(s) 260
PfeI GAWTC 1 cut(s) 696
PinAI ACCGGT 1 cut(s) 523
PkrI GCNGC 4 cut(s) 230, 233, 254, 355
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
PsiI TTATAA 1 cut(s) 675
PspN4I GGNNCC 2 cut(s) 301, 302
PspOMI GGGCCC 1 cut(s) 299
PspPI GGNCC 4 cut(s) 299, 300, 526, 604
SaqAI TTAA 4 cut(s) 513, 567, 627, 705
SatI GCNGC 4 cut(s) 229, 232, 253, 354
Sau96I GGNCC 4 cut(s) 299, 300, 526, 604
SchI GAGTC 1 cut(s) 185
SduI GDGCHC 1 cut(s) 303
SfaNI GCATC 1 cut(s) 325
SfcI CTRYAG 1 cut(s) 11
SinI GGWCC 2 cut(s) 526, 604
Sse9I AATT 5 cut(s) 91, 133, 357, 450, 650
SsiI CCGC 9 cut(s) 112, 228, 231, 234, 243, 252, 255, 353, 607
TaaI ACNGT 6 cut(s) 15, 224, 266, 346, 598, 710
TaqII GACCGA 1 cut(s) 369
TasI AATT 5 cut(s) 91, 133, 357, 450, 650
TauI GCSGC 4 cut(s) 231, 234, 255, 356
TfiI GAWTC 1 cut(s) 696
Tru1I TTAA 4 cut(s) 513, 567, 627, 705
Tru9I TTAA 4 cut(s) 513, 567, 627, 705
TscAI CASTG 1 cut(s) 229
TseFI GTSAC 1 cut(s) 260
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 5 cut(s) 376, 459, 553, 643, 660
TspRI CASTG 1 cut(s) 229
VpaK11BI GGWCC 2 cut(s) 526, 604
XapI RAATTY 1 cut(s) 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.