Rh2BG050100

Chloroplast processing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010175
Physical Location & Seq
Reverse (-)
3578843 .. 3579409
567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG050100.1

Sequence Viewer

Length: 567 bp
ATGCTCTCTCTACAGTTCTTCCCTCCATTACCTTCCCTCCAAAACCCTAATCCCGACCACACCCACTTCTCCATTCCCATCAAAAACCCCAATTTCACATCCCTCAATCTCCGCCCACTTTCCAAAACCCCCAATTCCAAGCTCTCAATCAAAACCCACTTCAGAAAACTCGTCTGCAATGCGTTGAAGGACTCCGGTAAAGAAAAGAAGGTGTCTTTGGACAGTGGCGGCGGCGGAGACGGCGGAGAAGGCGGTGGCGGTGACGGTGGCGATGATGAGCAAGGGGAGAAGAAAAGTGGGCCCCTGCCGGAATGGTTGAATATAACAACTGATGATGCCAAGACGGTGTTTGCGGCAATTGCAGTATCGCTTGCGTTTCGGTCTTTCATAGCTGAGCCGAGGTTTATTCCTTCCTTGTCAATGTATCCTACTTTGGATGTTGGGGATAGAATTGTAGCTGAAAAGGTTCGATTTTTGTTTCGGTTTTTCGGTGTGTGTGGTTGTGTTTTATGGATTCTGTTTGCTAAGGTTTGGTCATTGTTTTTGGTTTTGAAATTGAAATTGTAA

Protein Analysis

188

Amino Acids

20.59

Weight (kDa)

9.17

Isoelectric Point (pI)

27.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S26 PF10502 113 - 156 1.1e-06 Signal peptidase, peptidase S26
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 112, 228, 231, 234, 243, 252, 258, 353
AcuI CTGAAG 1 cut(s) 145
AgsI TTSAA 4 cut(s) 187, 319, 553, 559
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 3 cut(s) 142, 392, 458
AluI AGCT 3 cut(s) 142, 392, 458
Alw26I GTCTC 1 cut(s) 231
AoxI GGCC 1 cut(s) 299
ApaI GGGCCC 1 cut(s) 303
Asp700I GAANNNNTTC 1 cut(s) 465
AspS9I GGNCC 2 cut(s) 299, 300
AsuHPI GGTGA 1 cut(s) 272
BaeGI GKGCMC 1 cut(s) 303
BanII GRGCYC 1 cut(s) 303
BccI CCATC 1 cut(s) 86
BceAI ACGGC 1 cut(s) 256
BciVI GTATCC 1 cut(s) 435
BcoDI GTCTC 1 cut(s) 231
BfmI CTRYAG 1 cut(s) 11
BfuI GTATCC 1 cut(s) 435
BisI GCNGC 3 cut(s) 229, 232, 354
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 3 cut(s) 230, 233, 355
BmgT120I GGNCC 2 cut(s) 299, 300
BmiI GGNNCC 2 cut(s) 301, 302
BmsI GCATC 1 cut(s) 325
Bpu10I CCTNAGC 1 cut(s) 525
Bpu1102I GCTNAGC 1 cut(s) 393
BsaJI CCNNGG 1 cut(s) 398
BsaWI WCCGGW 1 cut(s) 194
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bse3DI GCAATG 1 cut(s) 184
BseDI CCNNGG 1 cut(s) 398
BseGI GGATG 2 cut(s) 98, 442
BseMI GCAATG 1 cut(s) 184
BseMII CTCAG 1 cut(s) 384
BseSI GKGCMC 1 cut(s) 303
BshFI GGCC 1 cut(s) 301
BsiSI CCGG 2 cut(s) 195, 308
BsmAI GTCTC 1 cut(s) 231
BsmBI CGTCTC 1 cut(s) 231
BsnI GGCC 1 cut(s) 301
Bsp120I GGGCCC 1 cut(s) 299
Bsp1286I GDGCHC 1 cut(s) 303
Bsp1720I GCTNAGC 1 cut(s) 393
BspACI CCGC 8 cut(s) 112, 228, 231, 234, 243, 252, 258, 353
BspANI GGCC 1 cut(s) 301
BspCNI CTCAG 1 cut(s) 385
BspLI GGNNCC 2 cut(s) 301, 302
BsrDI GCAATG 1 cut(s) 184
BssECI CCNNGG 1 cut(s) 398
Bst4CI ACNGT 4 cut(s) 15, 224, 266, 346
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 2 cut(s) 393, 525
BstF5I GGATG 2 cut(s) 98, 442
BstMAI GTCTC 1 cut(s) 231
BstMWI GCNNNNNNNGC 3 cut(s) 240, 249, 359
BstSFI CTRYAG 1 cut(s) 11
BstSLI GKGCMC 1 cut(s) 303
BsuI GTATCC 1 cut(s) 435
BsuRI GGCC 1 cut(s) 301
BtgZI GCGATG 1 cut(s) 285
BtsCI GGATG 2 cut(s) 98, 442
BtsIMutI CAGTG 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 372
Cfr13I GGNCC 2 cut(s) 299, 300
CviJI RGCY 5 cut(s) 142, 301, 392, 397, 458
CviKI_1 RGCY 5 cut(s) 142, 301, 392, 397, 458
DdeI CTNAG 2 cut(s) 393, 525
EciI GGCGGA 3 cut(s) 101, 249, 258
Eco24I GRGCYC 1 cut(s) 303
Eco57I CTGAAG 1 cut(s) 145
EcoO109I RGGNCCY 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 303
Esp3I CGTCTC 1 cut(s) 231
FaiI YATR 3 cut(s) 323, 389, 511
Fnu4HI GCNGC 3 cut(s) 229, 232, 354
FokI GGATG 2 cut(s) 85, 449
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 3 cut(s) 229, 232, 354
GluI GCNGC 3 cut(s) 229, 232, 354
HaeIII GGCC 1 cut(s) 301
HapII CCGG 2 cut(s) 195, 308
HinfI GANTC 2 cut(s) 191, 514
HpaII CCGG 2 cut(s) 195, 308
HphI GGTGA 1 cut(s) 272
Hpy188I TCNGA 1 cut(s) 164
Hpy188III TCNNGA 1 cut(s) 53
HpyAV CCTTC 5 cut(s) 42, 181, 202, 242, 420
HpyCH4III ACNGT 4 cut(s) 15, 224, 266, 346
HpyCH4V TGCA 2 cut(s) 177, 362
HpyF10VI GCNNNNNNNGC 3 cut(s) 240, 249, 359
HpyF3I CTNAG 2 cut(s) 393, 525
LpnPI CCDG 3 cut(s) 208, 317, 321
LweI GCATC 1 cut(s) 325
MaeIII GTNAC 1 cut(s) 260
MboII GAAGA 2 cut(s) 10, 301
MfeI CAATTG 1 cut(s) 357
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 6 cut(s) 91, 133, 357, 450, 554, 560
MlyI GAGTC 1 cut(s) 185
MnlI CCTC 4 cut(s) 33, 47, 113, 393
MroXI GAANNNNTTC 1 cut(s) 465
MspI CCGG 2 cut(s) 195, 308
MunI CAATTG 1 cut(s) 357
MwoI GCNNNNNNNGC 3 cut(s) 240, 249, 359
NlaIV GGNNCC 2 cut(s) 301, 302
NmeAIII GCCGAG 1 cut(s) 423
NmuCI GTSAC 1 cut(s) 260
PdmI GAANNNNTTC 1 cut(s) 465
PfeI GAWTC 1 cut(s) 514
PkrI GCNGC 3 cut(s) 230, 233, 355
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
PspN4I GGNNCC 2 cut(s) 301, 302
PspOMI GGGCCC 1 cut(s) 299
PspPI GGNCC 2 cut(s) 299, 300
SatI GCNGC 3 cut(s) 229, 232, 354
Sau96I GGNCC 2 cut(s) 299, 300
SchI GAGTC 1 cut(s) 185
SduI GDGCHC 1 cut(s) 303
SetI ASST 8 cut(s) 34, 144, 213, 394, 404, 460, 468, 531
SfaNI GCATC 1 cut(s) 325
SfcI CTRYAG 1 cut(s) 11
Sse9I AATT 6 cut(s) 91, 133, 357, 450, 554, 560
SsiI CCGC 8 cut(s) 112, 228, 231, 234, 243, 252, 258, 353
TaaI ACNGT 4 cut(s) 15, 224, 266, 346
TaqI TCGA 1 cut(s) 469
TaqII GACCGA 1 cut(s) 369
TasI AATT 6 cut(s) 91, 133, 357, 450, 554, 560
TauI GCSGC 3 cut(s) 231, 234, 356
TfiI GAWTC 1 cut(s) 514
TscAI CASTG 1 cut(s) 229
TseFI GTSAC 1 cut(s) 260
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 1 cut(s) 376
TspRI CASTG 1 cut(s) 229
XmnI GAANNNNTTC 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.