Rh3AG337800

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0010622
Physical Location & Seq
Reverse (-)
49303590 .. 49325502
21913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG337800.1

Sequence Viewer

Length: 432 bp
ATGCATCTCAGTTTGTATGACTATTGGAACTATTGGGGGTTGGAATTATTGGAAGCTTCAGTACCTGGATGTCCTACTCATTATCATGTTGTATATGATGAACTTGGCTTCTCAGCGGATGACTTGCATGATCTTGTTCATTCTTTATCATATGTTTACAAAAGAAGCACAATGGCCATATCTATAGATTCTTTGGATAGCTTATTTGGCGACAAAGATGCAAGTAGTGCTCTGGAAGTTTCTGGTATGATTCAACCAAAAGAGGAACCTGATTTACCTGATGGACCTGACGATGGAAAAAAGGCGTCCTTACTTATGATGAATTTTTCTAGAGATGAAGTTGAGTTTGCAATGAATATGCTTGAGGTGAATCTATTCCTCCCTGTAACACTACATTACTCATCATTCAAGTCGGATCATTTCAAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

143

Amino Acids

16.27

Weight (kDa)

4.58

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Piwi PF02171 25 - 62 7.3e-10 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 116
AclWI GGATC 1 cut(s) 423
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 1 cut(s) 322
AcuI CTGAAG 1 cut(s) 42
AcyI GRCGYC 1 cut(s) 305
AfaI GTAC 1 cut(s) 63
AfiI CCNNNNNNNGG 1 cut(s) 293
AgsI TTSAA 3 cut(s) 254, 409, 424
AjnI CCWGG 1 cut(s) 64
AluBI AGCT 2 cut(s) 56, 201
AluI AGCT 2 cut(s) 56, 201
Alw21I GWGCWC 1 cut(s) 232
AlwI GGATC 1 cut(s) 423
AlwNI CAGNNNCTG 1 cut(s) 65
AoxI GGCC 1 cut(s) 174
ApoI RAATTY 1 cut(s) 322
Asp700I GAANNNNTTC 1 cut(s) 374
AspS9I GGNCC 1 cut(s) 284
AsuHPI GGTGA 1 cut(s) 379
AvaII GGWCC 1 cut(s) 284
BalI TGGCCA 1 cut(s) 176
BarI GAAGNNNNNNTAC 2 cut(s) 45, 77
Bbv12I GWGCWC 1 cut(s) 232
BccI CCATC 2 cut(s) 275, 287
BcgI CGANNNNNNTGC 2 cut(s) 200, 234
BciT130I CCWGG 1 cut(s) 66
BfaI CTAG 1 cut(s) 330
BfmI CTRYAG 1 cut(s) 183
Bme1390I CCNGG 1 cut(s) 66
Bme18I GGWCC 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 284
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 66
BmsI GCATC 2 cut(s) 13, 208
BpuEI CTTGAG 1 cut(s) 383
BsaHI GRCGYC 1 cut(s) 305
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 1 cut(s) 66
BseGI GGATG 2 cut(s) 74, 124
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 2 cut(s) 22, 126
BshFI GGCC 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 232
BslI CCNNNNNNNGG 1 cut(s) 293
BsnI GGCC 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 232
Bsp143I GATC 2 cut(s) 130, 415
BspACI CCGC 1 cut(s) 116
BspANI GGCC 1 cut(s) 176
BspCNI CTCAG 2 cut(s) 21, 125
BspLI GGNNCC 1 cut(s) 267
BspPI GGATC 1 cut(s) 423
BsrDI GCAATG 1 cut(s) 357
BssMI GATC 2 cut(s) 130, 415
BssNI GRCGYC 1 cut(s) 305
Bst2UI CCWGG 1 cut(s) 66
BstACI GRCGYC 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 227
BstDEI CTNAG 2 cut(s) 8, 112
BstF5I GGATG 2 cut(s) 74, 124
BstKTI GATC 2 cut(s) 133, 418
BstMBI GATC 2 cut(s) 130, 415
BstMWI GCNNNNNNNGC 2 cut(s) 207, 227
BstNI CCWGG 1 cut(s) 66
BstSCI CCNGG 1 cut(s) 64
BstSFI CTRYAG 1 cut(s) 183
BsuRI GGCC 1 cut(s) 176
BtsCI GGATG 2 cut(s) 74, 124
CaiI CAGNNNCTG 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 284
CseI GACGC 1 cut(s) 294
Csp6I GTAC 1 cut(s) 62
CviAII CATG 2 cut(s) 86, 128
CviJI RGCY 4 cut(s) 56, 108, 176, 201
CviKI_1 RGCY 4 cut(s) 56, 108, 176, 201
CviQI GTAC 1 cut(s) 62
DdeI CTNAG 2 cut(s) 8, 112
DpnI GATC 2 cut(s) 132, 417
DpnII GATC 2 cut(s) 130, 415
EaeI YGGCCR 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 284
Eco57I CTGAAG 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 64
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 89, 131
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FatI CATG 2 cut(s) 85, 127
FauNDI CATATG 1 cut(s) 151
FokI GGATG 2 cut(s) 81, 131
FspBI CTAG 1 cut(s) 330
HaeIII GGCC 1 cut(s) 176
HgaI GACGC 1 cut(s) 294
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 2 cut(s) 89, 131
HindIII AAGCTT 1 cut(s) 54
HinfI GANTC 3 cut(s) 188, 250, 370
HphI GGTGA 1 cut(s) 379
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 1 cut(s) 415
Hpy188III TCNNGA 2 cut(s) 233, 330
Hpy8I GTNNAC 1 cut(s) 157
HpyCH4V TGCA 4 cut(s) 4, 127, 221, 350
HpyF10VI GCNNNNNNNGC 2 cut(s) 207, 227
HpyF3I CTNAG 2 cut(s) 8, 112
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 2 cut(s) 89, 131
Kzo9I GATC 2 cut(s) 130, 415
LpnPI CCDG 8 cut(s) 51, 78, 218, 228, 282, 291, 300, 396
LweI GCATC 2 cut(s) 13, 208
MaeI CTAG 1 cut(s) 330
MaeIII GTNAC 1 cut(s) 385
MalI GATC 2 cut(s) 132, 417
MboI GATC 2 cut(s) 130, 415
MhlI GDGCHC 1 cut(s) 232
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 2 cut(s) 44, 322
MluNI TGGCCA 1 cut(s) 176
MmeI TCCRAC 2 cut(s) 21, 393
MnlI CCTC 3 cut(s) 256, 358, 389
Mox20I TGGCCA 1 cut(s) 176
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 374
MscI TGGCCA 1 cut(s) 176
MslI CAYNNNNRTG 1 cut(s) 84
Msp20I TGGCCA 1 cut(s) 176
MspA1I CMGCKG 1 cut(s) 116
MspR9I CCNGG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 2 cut(s) 207, 227
NdeI CATATG 1 cut(s) 151
NdeII GATC 2 cut(s) 130, 415
NlaIII CATG 2 cut(s) 89, 131
NlaIV GGNNCC 1 cut(s) 267
NsiI ATGCAT 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 374
PfeI GAWTC 3 cut(s) 188, 250, 370
Psp6I CCWGG 1 cut(s) 64
PspGI CCWGG 1 cut(s) 64
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 1 cut(s) 284
PsrI GAACNNNNNNTAC 2 cut(s) 258, 290
PstNI CAGNNNCTG 1 cut(s) 65
RsaI GTAC 1 cut(s) 63
RsaNI GTAC 1 cut(s) 62
RseI CAYNNNNRTG 1 cut(s) 84
Sau3AI GATC 2 cut(s) 130, 415
Sau96I GGNCC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 1 cut(s) 232
SetI ASST 7 cut(s) 58, 67, 203, 271, 280, 289, 369
SfaNI GCATC 2 cut(s) 13, 208
SfcI CTRYAG 1 cut(s) 183
SinI GGWCC 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 2 cut(s) 44, 322
SsiI CCGC 1 cut(s) 116
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 1 cut(s) 64
TasI AATT 2 cut(s) 44, 322
TfiI GAWTC 3 cut(s) 188, 250, 370
TspDTI ATGAA 5 cut(s) 114, 128, 335, 351, 368
VpaK11BI GGWCC 1 cut(s) 284
XapI RAATTY 1 cut(s) 322
XbaI TCTAGA 1 cut(s) 329
XmnI GAANNNNTTC 1 cut(s) 374
XspI CTAG 1 cut(s) 330
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.