Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0005811

Physical Location & Seq

Genomic Coordinates Reverse (-)

21341079 .. 21363966

Gene Structure 22888 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG099900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGTCTATCGAAGAAACGAAAACTAGGGAATGGTCTAATCTTCCTACTGAACTTCTGGAATGCATAGCAGATCGTGCAGGCCTATTCGAGCTTCCAAGTATCTATCTTGTCTGCAAGGCTTGGTATTCTGCTTGTTCTACAACTTTAGCTCACATTGTTAAGCCTGAATTAAACCAAGGAGCTTGGTTTCTACTATATGAGGATAAAAGTTCTCAGTGCCAATTGATAACTGCAAGTGGTAGGAAGTTCACCACGAGTCTCCCTGAACTGGTTGGAACAACTTGCCTTGCAACATACCAAGCATGGCTTCTTCTTTTCAAAGGAGGATGTGCTTCAATGTTCTTCTTTCAACCTTTTTCTCGCCAAAGAATAGACCAACCAGGGCTCCCATTAGCGGAACTCACTGATCATCGTTTTGCAGCAGTTTCATGTCCACCAACTTTCAAAGATTGCATGGTTTGTGTGATCAACCGTAGGAATATTTATGAATGGGACGCCAACTTACTTTACCTTGGAAACCAGGCTTGGGTTGGAACACAGTGTAATCTTTCCCACACACGCCCGAATACAATCAAATGCGCTGTCTATGAAAATCAAACATTCAGATTTTACGATATCGTTCTAGGTCGACACATGCGCACCATGCTCGTCATATATCCTGACGGACGTTTCGCCTGGAATAAACCTTCAAAAGTAGTAGGTCGTTACGTACCACAGCATTTCCGGGACCAGAAAATACATCGTGATTTATTGATTTTTGTTTCTGGCAAGAATGAGTTGAAGAAAAAGTTGAAGGACATGAAGAACAAGCTGAGGTTGACAGAGGACGTTGTTTCTACTTGTGGGACATTTACACAGTTAGATGGCAAGCGCAAGCTTATTTTTAATGAGAGGCTCATGGATCCTGTTGACCAAAGTTTCAATGGAGTATGGACTGTGTGGATCCAGCCAAGTTCCTTTGGTGTACAGTTGGAGCACAAGTTAACAGAAGCAGAGGTTGAAAATCTAGCAAAAAAGAAATGGAGATATAAATGGGATGTGCCTGCTGCTGGAATGTCAAAGGGCAAATGGGCAGGAACAGGAGAGTGTTGCATAAAAATTGATGAATCTAATACCATGCAGCTGAACCATGTCTTCAGAACTAGAGATCTTGTAATGAATAATGATGCAAAAGTGGCCAAGCATCAGGAAAATGCTAAGAATGTAATTCTTACTGATGATAGTCTCCGTGATCATGGCTTTACTCGTCCTAAGGTGTTGATCCTACTGCCAATGGCGAGCATTGCACTTCGTGTTGTTAAGAGGATAATACAACTTACCCCTTCAGCTCACAAGGTTAATGTGGACCACATGGACCGATTTTCTAAAGAGTTCGGAAGTGAAGAGGTTCTTGACAATGAGGACGAAAGAGATATACCACCAGGTGACCAGAACTCTGGAAATGTAATACCTCACAAGTCATCGAAACCTAGTGACTTCCAAGTACTTTTTGGTGGCAATAGCAAGGATGATTTCATGGTTGGCATCAAGTTCACGAAGAGGAGCATAAAGTTGTACAGTGATTTTTATTCCTCAGATATCATAGTTGCTTCTGCTGTTGGTTTGTTGAAAAAAATTGATGAGGCTAAAATAAATAAGGAAAAGGATGTCGACTATCTTTCTTCTATAGAGGTTCTGATCATTGATCATGCAGATGTTATAACAATGCAGAACTGGGACTTTTTGAAGTCAGTTGTTGAGCAGTTAAACCATCAACCATCTAAGCAGCATGGAACTGATATCATGCGTATAAGGCCATGGTACCTGGATGGACATGCATCTTTTTATAGGCAAACTATACTCTTGAGTTATTATTCAAACCCAGATATAAACAATTTGTTTAACAAGCACTGCACAAATTACCGTGGGAAGGTGAAGTTGGTGCATGAATATAAAGGAGTTCTTCCTAAAGTGGTACTTCAAGTACGCCAGATTTTTCAGCGGTTTGAGGGAGATGAAATAGTGAATATTGATGATGCTCGTTTTGAACATTTTGCTACTAAGGTCTTCCCCAAGATAAAAAACTCTGTTGAGGGCGGGATTATGATATTTATCAGCTCTTACTTTGAATATGTTCAAGTTCGTAATTTTTTGAAGTCACAGAATGCATCCTTCTGTCTGCTTCATGAATATGCAGAGCAAAAAGATATTTCCCGTGCACGGGTTTGGTTCTTTGAAGGAAAGCGGAAGATCATGCTCTACACAGAGAGAGCACATTTTTACCACAGATACAAGATCCGCGGAATTCAGAATTTAATCGTCTATTCATTGCCGGAGAGAAAGGAATTTTACCCTGAGGTTGTTAATATGGTTGATGGATCCCACGACATGGCATGCACGGTGCTCTTTTCTCCTTTTGATCTCCTCCGGGCTGCTCCTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000462 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0019843 GO:0022613 GO:0030490 GO:0030515 GO:0030684 GO:0031974 GO:0031981 GO:0032040 GO:0032991 GO:0034470 GO:0034511 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UTP25

Protein Analysis

807

Amino Acids

93.21

Weight (kDa)

8.85

Isoelectric Point (pI)

32.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_KIB1-4	PF03478	83 - 239	1.4e-09	KIB1-4 beta-propeller
UTP25_NTPase-like	PF22916	374 - 638	1.4e-99	UTP25, NTP hydrolase-like domain
Utp25_C	PF06862	649 - 802	1.6e-53	Utp25, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005811)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17690
fragaria_vesca	FvH4_4g07640 FvH4_4g07640 FvH4_4g07640 FvH4_4g07640
malus_domestica	MD03G1036400.v1.1 MD13G1251100.v1.1 MD13G1251200.v1.1
prunus_persica	Prupe.1G079600_v2.0.a1 Prupe.1G079600_v2.0.a1
pyrus_communis	pycom03g02780 pycom13g21610 pycom13g22640 pycom13g22650
rosa_chinensis	RchiOBHm_Chr4g0400251
rosa_laevigata	RLG00000009200
rosa_multiflora	Rmu_co7994400.1_g000001 Rmu_sc0003908.1_g000001
rosa_roxburghii	Rroxscaffold_5G00345230
rosa_rugosa	Rorug04G0024200 Rorug04G0024300 Rorug04G0024400
rosa_samantha	Rh4AG099900 Rh4AG100300 Rh4BG097500 Rh4CG109100 Rh4DG094400
rosa_wichuraiana	Rw4G008260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1700
Acc16I	TGCGCA	1 cut(s)	638
Acc65I	GGTACC	1 cut(s)	1800
AccB1I	GGYRCC	1 cut(s)	1800
AccB7I	CCANNNNNTGG	1 cut(s)	2368
AccI	GTMKAC	2 cut(s)	628, 1650
AccII	CGCG	1 cut(s)	2280
AciI	CCGC	6 cut(s)	395, 1981, 2076, 2224, 2278, 2280
AclWI	GGATC	8 cut(s)	896, 909, 937, 950, 1255, 2269, 2352, 2365
AcoI	YGGCCR	1 cut(s)	1176
AcsI	RAATTY	3 cut(s)	2283, 2290, 2324
AcuI	CTGAAG	2 cut(s)	1120, 1308
AcyI	GRCGYC	1 cut(s)	495
AdeI	CACNNNGTG	2 cut(s)	1292, 1424
AfaI	GTAC	7 cut(s)	711, 966, 1485, 1556, 1802, 1956, 1965
AfiI	CCNNNNNNNGG	8 cut(s)	268, 394, 526, 1049, 1909, 2199, 2200, 2368
AjnI	CCWGG	5 cut(s)	379, 519, 674, 1420, 1803
AjuI	GAANNNNNNNTTGG	6 cut(s)	291, 323, 508, 540, 1901, 1933
AluBI	AGCT	8 cut(s)	91, 149, 182, 811, 877, 1123, 1328, 2097
AluI	AGCT	8 cut(s)	91, 149, 182, 811, 877, 1123, 1328, 2097
Alw21I	GWGCWC	4 cut(s)	978, 2200, 2254, 2385
Alw26I	GTCTC	2 cut(s)	263, 1229
Alw44I	GTGCAC	1 cut(s)	2196
AlwI	GGATC	8 cut(s)	896, 909, 937, 950, 1255, 2269, 2352, 2365
AoxI	GGCC	3 cut(s)	79, 1176, 1793
ApaLI	GTGCAC	1 cut(s)	2196
ApeKI	GCWGC	5 cut(s)	419, 1046, 1120, 1765, 2411
ApoI	RAATTY	3 cut(s)	2283, 2290, 2324
ArsI	GACNNNNNNTTYG	4 cut(s)	567, 599, 653, 685
Asp700I	GAANNNNTTC	2 cut(s)	1386, 2112
Asp718I	GGTACC	1 cut(s)	1800
AspLEI	GCGC	3 cut(s)	581, 639, 873
AspS9I	GGNCC	3 cut(s)	727, 1345, 1354
AsuC2I	CCSGG	2 cut(s)	725, 2408
AsuHPI	GGTGA	3 cut(s)	241, 1436, 1924
AvaII	GGWCC	3 cut(s)	727, 1345, 1354
AxyI	CCTNAGG	2 cut(s)	1251, 2334
BaeGI	GKGCMC	1 cut(s)	2200
BalI	TGGCCA	1 cut(s)	1178
BamHI	GGATCC	3 cut(s)	901, 942, 2357
BanI	GGYRCC	1 cut(s)	1800
BanII	GRGCYC	1 cut(s)	387
BauI	CACGAG	1 cut(s)	253
BbsI	GAAGAC	2 cut(s)	1126, 2038
Bbv12I	GWGCWC	4 cut(s)	978, 2200, 2254, 2385
BbvCI	CCTCAGC	1 cut(s)	812
BbvI	GCAGC	5 cut(s)	431, 1033, 1132, 1777, 2398
BccI	CCATC	5 cut(s)	857, 1758, 1765, 1802, 2348
BcgI	CGANNNNNNTGC	2 cut(s)	628, 662
BciT130I	CCWGG	5 cut(s)	381, 521, 676, 1422, 1805
BclI	TGATCA	5 cut(s)	406, 465, 1231, 1677, 1684
BcnI	CCSGG	2 cut(s)	725, 2408
BcoDI	GTCTC	2 cut(s)	263, 1229
BfaI	CTAG	5 cut(s)	24, 623, 1007, 1143, 1470
BfmI	CTRYAG	1 cut(s)	1665
BglII	AGATCT	1 cut(s)	1147
BisI	GCNGC	5 cut(s)	420, 1047, 1121, 1766, 2412
BlsI	GCNGC	5 cut(s)	421, 1048, 1122, 1767, 2413
BmcAI	AGTACT	1 cut(s)	1485
Bme1390I	CCNGG	7 cut(s)	381, 521, 676, 725, 1422, 1805, 2408
Bme18I	GGWCC	3 cut(s)	727, 1345, 1354
BmgT120I	GGNCC	3 cut(s)	727, 1345, 1354
BmiI	GGNNCC	6 cut(s)	386, 728, 903, 944, 1802, 2359
BmrFI	CCNGG	7 cut(s)	381, 521, 676, 725, 1422, 1805, 2408
BmrI	ACTGGG	1 cut(s)	1723
BmsI	GCATC	6 cut(s)	1156, 1192, 1533, 1826, 2005, 2156
BmuI	ACTGGG	1 cut(s)	1723
BpiI	GAAGAC	2 cut(s)	1126, 2038
Bpu10I	CCTNAGC	1 cut(s)	812
BpuEI	CTTGAG	1 cut(s)	1864
BpuMI	CCSGG	2 cut(s)	725, 2408
BsaAI	YACGTR	1 cut(s)	709
BsaBI	GATNNNNATC	1 cut(s)	2090
BsaHI	GRCGYC	1 cut(s)	495
BsaJI	CCNNGG	6 cut(s)	175, 380, 511, 1796, 1903, 2278
BsaXI	ACNNNNNCTCC	4 cut(s)	369, 399, 965, 995
Bsc4I	CCNNNNNNNGG	8 cut(s)	268, 394, 526, 1049, 1909, 2199, 2200, 2368
Bse1I	ACTGG	2 cut(s)	273, 1718
Bse21I	CCTNAGG	2 cut(s)	1251, 2334
Bse3DI	GCAATG	2 cut(s)	1281, 2306
Bse8I	GATNNNNATC	1 cut(s)	2090
BseBI	CCWGG	5 cut(s)	381, 521, 676, 1422, 1805
BseDI	CCNNGG	6 cut(s)	175, 380, 511, 1796, 1903, 2278
BseGI	GGATG	6 cut(s)	332, 1042, 1513, 1651, 1813, 2147
BseJI	GATNNNNATC	1 cut(s)	2090
BseLI	CCNNNNNNNGG	8 cut(s)	268, 394, 526, 1049, 1909, 2199, 2200, 2368
BseMI	GCAATG	2 cut(s)	1281, 2306
BseMII	CTCAG	4 cut(s)	227, 803, 1587, 2325
BseNI	ACTGG	2 cut(s)	273, 1718
BseRI	GAGGAG	3 cut(s)	1555, 2393, 2406
BseSI	GKGCMC	1 cut(s)	2200
BseXI	GCAGC	5 cut(s)	431, 1033, 1132, 1777, 2398
BsgI	GTGCAG	2 cut(s)	96, 1876
Bsh1236I	CGCG	1 cut(s)	2280
BshFI	GGCC	3 cut(s)	81, 1178, 1795
BshNI	GGYRCC	1 cut(s)	1800
BsiHKAI	GWGCWC	4 cut(s)	978, 2200, 2254, 2385
BsiSI	CCGG	3 cut(s)	724, 2312, 2407
BslFI	GGGAC	4 cut(s)	506, 740, 859, 1730
BslI	CCNNNNNNNGG	8 cut(s)	268, 394, 526, 1049, 1909, 2199, 2200, 2368
BsmAI	GTCTC	2 cut(s)	263, 1229
BsmFI	GGGAC	4 cut(s)	506, 740, 859, 1730
BsmI	GAATGC	2 cut(s)	65, 2149
BsnI	GGCC	3 cut(s)	81, 1178, 1795
Bsp1286I	GDGCHC	5 cut(s)	387, 978, 2200, 2254, 2385
Bsp1407I	TGTACA	2 cut(s)	964, 1554
Bsp19I	CCATGG	1 cut(s)	1796
BspACI	CCGC	6 cut(s)	395, 1981, 2076, 2224, 2278, 2280
BspANI	GGCC	3 cut(s)	81, 1178, 1795
BspCNI	CTCAG	4 cut(s)	226, 804, 1586, 2326
BspFNI	CGCG	1 cut(s)	2280
BspHI	TCATGA	1 cut(s)	2164
BspLI	GGNNCC	6 cut(s)	386, 728, 903, 944, 1802, 2359
BspPI	GGATC	8 cut(s)	896, 909, 937, 950, 1255, 2269, 2352, 2365
BspT107I	GGYRCC	1 cut(s)	1800
BsrDI	GCAATG	2 cut(s)	1281, 2306
BsrGI	TGTACA	2 cut(s)	964, 1554
BsrI	ACTGG	2 cut(s)	273, 1718
BssECI	CCNNGG	6 cut(s)	175, 380, 511, 1796, 1903, 2278
BssNI	GRCGYC	1 cut(s)	495
BssSI	CACGAG	1 cut(s)	253
BssT1I	CCWWGG	3 cut(s)	175, 511, 1796
Bst2BI	CACGAG	1 cut(s)	253
Bst2UI	CCWGG	5 cut(s)	381, 521, 676, 1422, 1805
Bst4CI	ACNGT	8 cut(s)	473, 540, 858, 937, 969, 1559, 1904, 2380
Bst6I	CTCTTC	2 cut(s)	1377, 1532
BstACI	GRCGYC	1 cut(s)	495
BstAPI	GCANNNNNTGC	1 cut(s)	74
BstAUI	TGTACA	2 cut(s)	964, 1554
BstBAI	YACGTR	1 cut(s)	709
BstC8I	GCNNGC	6 cut(s)	79, 869, 875, 1044, 1279, 2374
BstDEI	CTNAG	8 cut(s)	213, 812, 1197, 1251, 1573, 1761, 2040, 2334
BstDSI	CCRYGG	3 cut(s)	1796, 1903, 2278
BstEII	GGTNACC	1 cut(s)	1424
BstF5I	GGATG	6 cut(s)	332, 1042, 1513, 1651, 1813, 2147
BstFNI	CGCG	1 cut(s)	2280
BstHHI	GCGC	3 cut(s)	581, 639, 873
BstMAI	GTCTC	2 cut(s)	263, 1229
BstMWI	GCNNNNNNNGC	5 cut(s)	74, 643, 1175, 1283, 1792
BstNI	CCWGG	5 cut(s)	381, 521, 676, 1422, 1805
BstNSI	RCATGY	3 cut(s)	637, 1817, 2376
BstPI	GGTNACC	1 cut(s)	1424
BstSCI	CCNGG	7 cut(s)	379, 519, 674, 723, 1420, 1803, 2406
BstSFI	CTRYAG	1 cut(s)	1665
BstSLI	GKGCMC	1 cut(s)	2200
BstSNI	TACGTA	1 cut(s)	709
BstUI	CGCG	1 cut(s)	2280
BstV1I	GCAGC	5 cut(s)	431, 1033, 1132, 1777, 2398
BstV2I	GAAGAC	2 cut(s)	1126, 2038
BstX2I	RGATCY	5 cut(s)	901, 942, 1147, 2274, 2357
BstXI	CCANNNNNNTGG	1 cut(s)	1436
BstYI	RGATCY	5 cut(s)	901, 942, 1147, 2274, 2357
Bsu36I	CCTNAGG	2 cut(s)	1251, 2334
BsuRI	GGCC	3 cut(s)	81, 1178, 1795
BtgI	CCRYGG	3 cut(s)	1796, 1903, 2278
BtsCI	GGATG	6 cut(s)	332, 1042, 1513, 1651, 1813, 2147
BtsI	GCAGTG	1 cut(s)	1888
BtsIMutI	CAGTG	5 cut(s)	221, 402, 545, 1564, 1888
Cac8I	GCNNGC	6 cut(s)	79, 869, 875, 1044, 1279, 2374
CciI	TCATGA	1 cut(s)	2164
CfoI	GCGC	3 cut(s)	581, 639, 873
Cfr13I	GGNCC	3 cut(s)	727, 1345, 1354
Cfr42I	CCGCGG	1 cut(s)	2281
CseI	GACGC	1 cut(s)	503
CsiI	ACCWGGT	1 cut(s)	1420
Csp6I	GTAC	7 cut(s)	710, 965, 1484, 1555, 1801, 1955, 1964
CviQI	GTAC	7 cut(s)	710, 965, 1484, 1555, 1801, 1955, 1964
DdeI	CTNAG	8 cut(s)	213, 812, 1197, 1251, 1573, 1761, 2040, 2334
DraIII	CACNNNGTG	2 cut(s)	1292, 1424
EaeI	YGGCCR	1 cut(s)	1176
Eam1104I	CTCTTC	2 cut(s)	1377, 1532
EarI	CTCTTC	2 cut(s)	1377, 1532
Eco105I	TACGTA	1 cut(s)	709
Eco130I	CCWWGG	3 cut(s)	175, 511, 1796
Eco147I	AGGCCT	1 cut(s)	81
Eco24I	GRGCYC	1 cut(s)	387
Eco32I	GATATC	3 cut(s)	616, 1579, 1780
Eco47I	GGWCC	3 cut(s)	727, 1345, 1354
Eco57I	CTGAAG	2 cut(s)	1120, 1308
Eco81I	CCTNAGG	2 cut(s)	1251, 2334
Eco91I	GGTNACC	1 cut(s)	1424
EcoO65I	GGTNACC	1 cut(s)	1424
EcoRI	GAATTC	1 cut(s)	2283
EcoRII	CCWGG	5 cut(s)	379, 519, 674, 1420, 1803
EcoRV	GATATC	3 cut(s)	616, 1579, 1780
EcoT14I	CCWWGG	3 cut(s)	175, 511, 1796
EcoT22I	ATGCAT	3 cut(s)	65, 1819, 2149
EcoT38I	GRGCYC	1 cut(s)	387
ErhI	CCWWGG	3 cut(s)	175, 511, 1796
FalI	AAGNNNNNCTT	8 cut(s)	291, 323, 1374, 1406, 1926, 1958, 1941, 1973
FaqI	GGGAC	4 cut(s)	506, 740, 859, 1730
FauI	CCCGC	1 cut(s)	2069
FbaI	TGATCA	5 cut(s)	406, 465, 1231, 1677, 1684
FblI	GTMKAC	2 cut(s)	628, 1650
Fnu4HI	GCNGC	5 cut(s)	420, 1047, 1121, 1766, 2412
FokI	GGATG	6 cut(s)	339, 1049, 1520, 1658, 1820, 2134
FriOI	GRGCYC	1 cut(s)	387
Fsp4HI	GCNGC	5 cut(s)	420, 1047, 1121, 1766, 2412
FspAI	RTGCGCAY	1 cut(s)	638
FspBI	CTAG	5 cut(s)	24, 623, 1007, 1143, 1470
FspI	TGCGCA	1 cut(s)	638
GlaI	GCGC	3 cut(s)	580, 638, 872
GluI	GCNGC	5 cut(s)	420, 1047, 1121, 1766, 2412
HaeIII	GGCC	3 cut(s)	81, 1178, 1795
HapII	CCGG	3 cut(s)	724, 2312, 2407
HgaI	GACGC	1 cut(s)	503
HhaI	GCGC	3 cut(s)	581, 639, 873
Hin1I	GRCGYC	1 cut(s)	495
Hin6I	GCGC	3 cut(s)	579, 637, 871
HinP1I	GCGC	3 cut(s)	579, 637, 871
HincII	GTYRAC	5 cut(s)	629, 819, 910, 984, 1651
HindII	GTYRAC	5 cut(s)	629, 819, 910, 984, 1651
HindIII	AAGCTT	1 cut(s)	875
HinfI	GANTC	2 cut(s)	256, 1106
HpaI	GTTAAC	1 cut(s)	984
HpaII	CCGG	3 cut(s)	724, 2312, 2407
HphI	GGTGA	3 cut(s)	241, 1436, 1924
Hpy188I	TCNGA	6 cut(s)	605, 1139, 1376, 1576, 1677, 2289
Hpy188III	TCNNGA	9 cut(s)	56, 659, 743, 1187, 1391, 1437, 1534, 1843, 2165
HpyAV	CCTTC	6 cut(s)	696, 787, 1332, 1903, 2161, 2210
HpyCH4III	ACNGT	8 cut(s)	473, 540, 858, 937, 969, 1559, 1904, 2380
HpyCH4IV	ACGT	3 cut(s)	667, 708, 828
HpyF10VI	GCNNNNNNNGC	5 cut(s)	74, 643, 1175, 1283, 1792
HpyF3I	CTNAG	8 cut(s)	213, 812, 1197, 1251, 1573, 1761, 2040, 2334
HpySE526I	ACGT	3 cut(s)	667, 708, 828
Hsp92I	GRCGYC	1 cut(s)	495
HspAI	GCGC	3 cut(s)	579, 637, 871
KpnI	GGTACC	1 cut(s)	1804
Ksp22I	TGATCA	5 cut(s)	406, 465, 1231, 1677, 1684
KspAI	GTTAAC	1 cut(s)	984
KspI	CCGCGG	1 cut(s)	2281
LmnI	GCTCC	5 cut(s)	179, 390, 973, 1542, 2419
Lsp1109I	GCAGC	5 cut(s)	431, 1033, 1132, 1777, 2398
LweI	GCATC	6 cut(s)	1156, 1192, 1533, 1826, 2005, 2156
MabI	ACCWGGT	1 cut(s)	1420
MaeI	CTAG	5 cut(s)	24, 623, 1007, 1143, 1470
MaeII	ACGT	3 cut(s)	667, 708, 828
MaeIII	GTNAC	4 cut(s)	704, 1424, 1472, 2136
MfeI	CAATTG	1 cut(s)	221
MflI	RGATCY	5 cut(s)	901, 942, 1147, 2274, 2357
MhlI	GDGCHC	5 cut(s)	387, 978, 2200, 2254, 2385
MlsI	TGGCCA	1 cut(s)	1178
MluNI	TGGCCA	1 cut(s)	1178
MlyI	GAGTC	1 cut(s)	265
MmeI	TCCRAC	3 cut(s)	253, 511, 951
Mox20I	TGGCCA	1 cut(s)	1178
Mph1103I	ATGCAT	3 cut(s)	65, 1819, 2149
MroXI	GAANNNNTTC	2 cut(s)	1386, 2112
MscI	TGGCCA	1 cut(s)	1178
MslI	CAYNNNNRTG	2 cut(s)	1692, 2169
Msp20I	TGGCCA	1 cut(s)	1178
MspA1I	CMGCKG	3 cut(s)	1123, 1981, 2280
MspI	CCGG	3 cut(s)	724, 2312, 2407
MspR9I	CCNGG	7 cut(s)	381, 521, 676, 725, 1422, 1805, 2408
MunI	CAATTG	1 cut(s)	221
Mva1269I	GAATGC	2 cut(s)	65, 2149
MvaI	CCWGG	5 cut(s)	381, 521, 676, 1422, 1805
MvnI	CGCG	1 cut(s)	2280
MwoI	GCNNNNNNNGC	5 cut(s)	74, 643, 1175, 1283, 1792
NciI	CCSGG	2 cut(s)	725, 2408
NcoI	CCATGG	1 cut(s)	1796
NlaIV	GGNNCC	6 cut(s)	386, 728, 903, 944, 1802, 2359
NmuCI	GTSAC	3 cut(s)	1424, 1472, 2136
NsbI	TGCGCA	1 cut(s)	638
NsiI	ATGCAT	3 cut(s)	65, 1819, 2149
NspI	RCATGY	3 cut(s)	637, 1817, 2376
PaeI	GCATGC	1 cut(s)	2376
PagI	TCATGA	1 cut(s)	2164
PceI	AGGCCT	1 cut(s)	81
PctI	GAATGC	2 cut(s)	65, 2149
PdmI	GAANNNNTTC	2 cut(s)	1386, 2112
PfeI	GAWTC	1 cut(s)	1106
PflMI	CCANNNNNTGG	1 cut(s)	2368
PfoI	TCCNGGA	1 cut(s)	723
PkrI	GCNGC	5 cut(s)	421, 1048, 1122, 1767, 2413
PleI	GAGTC	1 cut(s)	264
PpsI	GAGTC	1 cut(s)	264
Ppu21I	YACGTR	1 cut(s)	709
PsiI	TTATAA	1 cut(s)	1700
Psp6I	CCWGG	5 cut(s)	379, 519, 674, 1420, 1803
PspEI	GGTNACC	1 cut(s)	1424
PspGI	CCWGG	5 cut(s)	379, 519, 674, 1420, 1803
PspN4I	GGNNCC	6 cut(s)	386, 728, 903, 944, 1802, 2359
PspPI	GGNCC	3 cut(s)	727, 1345, 1354
PsuI	RGATCY	5 cut(s)	901, 942, 1147, 2274, 2357
PvuII	CAGCTG	1 cut(s)	1123
RsaI	GTAC	7 cut(s)	711, 966, 1485, 1556, 1802, 1956, 1965
RsaNI	GTAC	7 cut(s)	710, 965, 1484, 1555, 1801, 1955, 1964
RseI	CAYNNNNRTG	2 cut(s)	1692, 2169
SacII	CCGCGG	1 cut(s)	2281
SalI	GTCGAC	2 cut(s)	627, 1649
SatI	GCNGC	5 cut(s)	420, 1047, 1121, 1766, 2412
Sau96I	GGNCC	3 cut(s)	727, 1345, 1354
ScaI	AGTACT	1 cut(s)	1485
SchI	GAGTC	1 cut(s)	265
ScrFI	CCNGG	7 cut(s)	381, 521, 676, 725, 1422, 1805, 2408
SduI	GDGCHC	5 cut(s)	387, 978, 2200, 2254, 2385
SexAI	ACCWGGT	1 cut(s)	1420
SfaNI	GCATC	6 cut(s)	1156, 1192, 1533, 1826, 2005, 2156
SfcI	CTRYAG	1 cut(s)	1665
Sfr303I	CCGCGG	1 cut(s)	2281
SgrBI	CCGCGG	1 cut(s)	2281
SinI	GGWCC	3 cut(s)	727, 1345, 1354
SmiMI	CAYNNNNRTG	2 cut(s)	1692, 2169
SmlI	CTYRAG	1 cut(s)	1843
SmoI	CTYRAG	1 cut(s)	1843
SnaBI	TACGTA	1 cut(s)	709
SphI	GCATGC	1 cut(s)	2376
SseBI	AGGCCT	1 cut(s)	81
SsiI	CCGC	6 cut(s)	395, 1981, 2076, 2224, 2278, 2280
SspI	AATATT	2 cut(s)	481, 2008
SspMI	CTAG	5 cut(s)	24, 623, 1007, 1143, 1470
StuI	AGGCCT	1 cut(s)	81
StyD4I	CCNGG	7 cut(s)	379, 519, 674, 723, 1420, 1803, 2406
StyI	CCWWGG	3 cut(s)	175, 511, 1796
TaaI	ACNGT	8 cut(s)	473, 540, 858, 937, 969, 1559, 1904, 2380
TaiI	ACGT	3 cut(s)	670, 711, 831
TaqI	TCGA	5 cut(s)	9, 87, 628, 1463, 1650
TaqII	GACCGA	1 cut(s)	1371
TatI	WGTACW	3 cut(s)	964, 1483, 1554
TfiI	GAWTC	1 cut(s)	1106
TscAI	CASTG	5 cut(s)	221, 409, 545, 1564, 1895
TseFI	GTSAC	3 cut(s)	1424, 1472, 2136
TseI	GCWGC	5 cut(s)	419, 1046, 1120, 1765, 2411
Tsp45I	GTSAC	3 cut(s)	1424, 1472, 2136
TspGWI	ACGGA	2 cut(s)	678, 1217
TspRI	CASTG	5 cut(s)	221, 409, 545, 1564, 1895
Van91I	CCANNNNNTGG	1 cut(s)	2368
VneI	GTGCAC	1 cut(s)	2196
VpaK11BI	GGWCC	3 cut(s)	727, 1345, 1354
XapI	RAATTY	3 cut(s)	2283, 2290, 2324
XceI	RCATGY	3 cut(s)	637, 1817, 2376
XcmI	CCANNNNNNNNNTGG	3 cut(s)	527, 920, 1487
XmiI	GTMKAC	2 cut(s)	628, 1650
XmnI	GAANNNNTTC	2 cut(s)	1386, 2112
XspI	CTAG	5 cut(s)	24, 623, 1007, 1143, 1470
ZrmI	AGTACT	1 cut(s)	1485
Zsp2I	ATGCAT	3 cut(s)	65, 1819, 2149

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.