Rh4CG286500

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0015753
Physical Location & Seq
Forward (+)
54408132 .. 54413888
5757 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG286500.1

Sequence Viewer

Length: 426 bp
ATGGAGCCTGGATCTACGACCCTAATGCTTCTCCCAAGTACGACAACACCTGCAAGGAGATCTTCAAGGGCTGGAATTGCATTTCCAACAACAAATCCAACGGCCGCGAACTCATCAAGTGGAGGTGGAAGCCTAAGCACTTCAAGAGACTTGAGCCACTCGCCACAATTGTACCCGCCGGATGTCGACCACGGCGGGATTTTGTACACTGTGACCTCGCGCACCACTAGCAGCTTAAGAATCCTCGTCGTCGCCACCGCCGCGATCCATCTCCTTCGTCACTATAAGCTCGATCTCCCTGACGATCTCCTCTCCTCCAAACCCTCCGATCAGTCCTGGACCTCCAAAGATCTTCTAAAAGGCTGTGCAATTGATGGGGGATATAATGATAAAGTTTTATATCATTTGTTTTCATTTAAAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

141

Amino Acids

15.08

Weight (kDa)

8.79

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 58
Acc36I ACCTGC 1 cut(s) 58
AccI GTMKAC 1 cut(s) 186
AccII CGCG 3 cut(s) 107, 220, 263
AciI CCGC 5 cut(s) 105, 176, 195, 258, 261
AclWI GGATC 2 cut(s) 19, 259
AcoI YGGCCR 1 cut(s) 102
AfaI GTAC 3 cut(s) 40, 173, 206
AflII CTTAAG 1 cut(s) 235
AgsI TTSAA 2 cut(s) 66, 144
AjnI CCWGG 2 cut(s) 7, 335
AluBI AGCT 2 cut(s) 234, 289
AluI AGCT 2 cut(s) 234, 289
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 2 cut(s) 19, 259
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 1 cut(s) 231
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 1 cut(s) 339
AvaII GGWCC 1 cut(s) 339
BaeI ACNNNNGTAYC 2 cut(s) 155, 188
BbvI GCAGC 1 cut(s) 243
BccI CCATC 2 cut(s) 276, 368
BceAI ACGGC 2 cut(s) 117, 208
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BciT130I CCWGG 2 cut(s) 9, 337
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 1 cut(s) 228
BfrI CTTAAG 1 cut(s) 235
BfuAI ACCTGC 1 cut(s) 58
BglII AGATCT 2 cut(s) 59, 349
BisI GCNGC 3 cut(s) 105, 232, 261
BlsI GCNGC 3 cut(s) 106, 233, 262
Bme1390I CCNGG 2 cut(s) 9, 337
Bme18I GGWCC 1 cut(s) 339
BmgT120I GGNCC 1 cut(s) 339
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 9, 337
Bpu10I CCTNAGC 1 cut(s) 134
BpuEI CTTGAG 1 cut(s) 172
BsaJI CCNNGG 1 cut(s) 190
BseBI CCWGG 2 cut(s) 9, 337
BseDI CCNNGG 1 cut(s) 190
BseGI GGATG 1 cut(s) 187
BseRI GAGGAG 2 cut(s) 299, 304
BseX3I CGGCCG 1 cut(s) 102
BseXI GCAGC 1 cut(s) 243
Bsh1236I CGCG 3 cut(s) 107, 220, 263
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 1 cut(s) 104
BsiEI CGRYCG 1 cut(s) 105
BsiSI CCGG 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 141
BsnI GGCC 1 cut(s) 104
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 7 cut(s) 11, 59, 264, 292, 304, 328, 349
BspACI CCGC 5 cut(s) 105, 176, 195, 258, 261
BspANI GGCC 1 cut(s) 104
BspFNI CGCG 3 cut(s) 107, 220, 263
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 58
BspPI GGATC 2 cut(s) 19, 259
BspTI CTTAAG 1 cut(s) 235
BsrGI TGTACA 1 cut(s) 204
BssECI CCNNGG 1 cut(s) 190
BssMI GATC 7 cut(s) 11, 59, 264, 292, 304, 328, 349
Bst2UI CCWGG 2 cut(s) 9, 337
Bst4CI ACNGT 1 cut(s) 211
BstAFI CTTAAG 1 cut(s) 235
BstAUI TGTACA 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 134
BstDSI CCRYGG 1 cut(s) 190
BstF5I GGATG 1 cut(s) 187
BstFNI CGCG 3 cut(s) 107, 220, 263
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 7 cut(s) 14, 62, 267, 295, 307, 331, 352
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 7 cut(s) 11, 59, 264, 292, 304, 328, 349
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 3 cut(s) 77, 228, 260
BstNI CCWGG 2 cut(s) 9, 337
BstSCI CCNGG 2 cut(s) 7, 335
BstUI CGCG 3 cut(s) 107, 220, 263
BstV1I GCAGC 1 cut(s) 243
BstX2I RGATCY 3 cut(s) 11, 59, 349
BstYI RGATCY 3 cut(s) 11, 59, 349
BstZI CGGCCG 1 cut(s) 102
BsuRI GGCC 1 cut(s) 104
BtgI CCRYGG 1 cut(s) 190
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 207
BveI ACCTGC 1 cut(s) 58
CfoI GCGC 1 cut(s) 222
Cfr13I GGNCC 1 cut(s) 339
Csp6I GTAC 3 cut(s) 39, 172, 205
CviJI RGCY 8 cut(s) 7, 71, 104, 132, 156, 234, 289, 363
CviKI_1 RGCY 8 cut(s) 7, 71, 104, 132, 156, 234, 289, 363
CviQI GTAC 3 cut(s) 39, 172, 205
DdeI CTNAG 1 cut(s) 134
DpnI GATC 7 cut(s) 13, 61, 266, 294, 306, 330, 351
DpnII GATC 7 cut(s) 11, 59, 264, 292, 304, 328, 349
DraI TTTAAA 1 cut(s) 418
EaeI YGGCCR 1 cut(s) 102
EagI CGGCCG 1 cut(s) 102
EclXI CGGCCG 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 339
Eco52I CGGCCG 1 cut(s) 102
EcoRII CCWGG 2 cut(s) 7, 335
FaiI YATR 3 cut(s) 285, 384, 400
FalI AAGNNNNNCTT 2 cut(s) 46, 78
FauI CCCGC 2 cut(s) 183, 188
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 3 cut(s) 105, 232, 261
FokI GGATG 1 cut(s) 194
Fsp4HI GCNGC 3 cut(s) 105, 232, 261
FspBI CTAG 1 cut(s) 228
GlaI GCGC 1 cut(s) 221
GluI GCNGC 3 cut(s) 105, 232, 261
HaeIII GGCC 1 cut(s) 104
HapII CCGG 1 cut(s) 179
HhaI GCGC 1 cut(s) 222
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 1 cut(s) 240
HpaII CCGG 1 cut(s) 179
Hpy166II GTNNAC 2 cut(s) 187, 207
Hpy188I TCNGA 1 cut(s) 328
Hpy188III TCNNGA 1 cut(s) 144
Hpy8I GTNNAC 2 cut(s) 187, 207
Hpy99I CGWCG 2 cut(s) 251, 254
HpyAV CCTTC 1 cut(s) 284
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4V TGCA 3 cut(s) 53, 80, 368
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 228, 260
HpyF3I CTNAG 1 cut(s) 134
HspAI GCGC 1 cut(s) 220
Kzo9I GATC 7 cut(s) 11, 59, 264, 292, 304, 328, 349
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 7 cut(s) 21, 57, 63, 192, 312, 322, 349
Lsp1109I GCAGC 1 cut(s) 243
MaeI CTAG 1 cut(s) 228
MaeIII GTNAC 2 cut(s) 211, 278
MalI GATC 7 cut(s) 13, 61, 266, 294, 306, 330, 351
MboI GATC 7 cut(s) 11, 59, 264, 292, 304, 328, 349
MboII GAAGA 2 cut(s) 54, 344
MfeI CAATTG 2 cut(s) 167, 369
MflI RGATCY 3 cut(s) 11, 59, 349
MluCI AATT 3 cut(s) 75, 167, 369
MmeI TCCRAC 2 cut(s) 110, 122
MnlI CCTC 7 cut(s) 116, 226, 254, 320, 325, 334, 352
MseI TTAA 2 cut(s) 236, 417
MspCI CTTAAG 1 cut(s) 235
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 2 cut(s) 9, 337
MunI CAATTG 2 cut(s) 167, 369
MvaI CCWGG 2 cut(s) 9, 337
MvnI CGCG 3 cut(s) 107, 220, 263
MwoI GCNNNNNNNGC 3 cut(s) 77, 228, 260
NdeII GATC 7 cut(s) 11, 59, 264, 292, 304, 328, 349
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 211, 278
PaqCI CACCTGC 1 cut(s) 58
PfeI GAWTC 1 cut(s) 240
PfoI TCCNGGA 1 cut(s) 335
PkrI GCNGC 3 cut(s) 106, 233, 262
Psp6I CCWGG 2 cut(s) 7, 335
PspGI CCWGG 2 cut(s) 7, 335
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 339
PsuI RGATCY 3 cut(s) 11, 59, 349
RsaI GTAC 3 cut(s) 40, 173, 206
RsaNI GTAC 3 cut(s) 39, 172, 205
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 2 cut(s) 236, 417
SatI GCNGC 3 cut(s) 105, 232, 261
Sau3AI GATC 7 cut(s) 11, 59, 264, 292, 304, 328, 349
Sau96I GGNCC 1 cut(s) 339
ScrFI CCNGG 2 cut(s) 9, 337
SetI ASST 6 cut(s) 52, 127, 218, 236, 291, 344
SinI GGWCC 1 cut(s) 339
SmlI CTYRAG 2 cut(s) 151, 235
SmoI CTYRAG 2 cut(s) 151, 235
Sse9I AATT 3 cut(s) 75, 167, 369
SsiI CCGC 5 cut(s) 105, 176, 195, 258, 261
SspMI CTAG 1 cut(s) 228
StyD4I CCNGG 2 cut(s) 7, 335
TaaI ACNGT 1 cut(s) 211
TaqI TCGA 2 cut(s) 186, 291
TasI AATT 3 cut(s) 75, 167, 369
TatI WGTACW 1 cut(s) 204
TauI GCSGC 2 cut(s) 107, 263
TfiI GAWTC 1 cut(s) 240
Tru1I TTAA 2 cut(s) 236, 417
Tru9I TTAA 2 cut(s) 236, 417
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 2 cut(s) 211, 278
TseI GCWGC 1 cut(s) 231
Tsp45I GTSAC 2 cut(s) 211, 278
TspDTI ATGAA 1 cut(s) 402
TspRI CASTG 1 cut(s) 214
Vha464I CTTAAG 1 cut(s) 235
VpaK11BI GGWCC 1 cut(s) 339
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.