Rh5AG508300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0005495
Physical Location & Seq
Reverse (-)
85895438 .. 85896435
998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG508300.1

Sequence Viewer

Length: 990 bp
ATGGCGGGAATAGCAATTGTCTTAGATCTGCTGAGACAAAACCCAAGTCTTACAACCCAAAGCCTACACGCTTCTGGGTTTTTCTCCGCTAAAGCAGCCGCTGCCGCCACCTCCGTCGCCGCTGGAGCTCCTTACGTTTACAAGTCCTTCTTCAGTAATTTTAGGGTTCCAGTTGCCTATTGTGATGCTGGAGCAACATTGTCTGAAGATTACACCTCTAATGTACGAAGTGCATCCTTAAAAATATTTCAGAATGAATCTGTGAATTTCGGTACCAAGGAATACACAATCGAGTTAAAGCCTCTATTCTCAGCTTTCGAATTGAGAGCTCTAACTCTGACGACTCTGAGGTCATTTTTGATGTTCTACTTACCTCTTCTGGAGCCTGGTAGAAACTTGCAAGAGGACGATGATGACTTCCTGCGGGACACTGAAGAAGAACAACATGTAGATTATGTTGCTCCATTCAAAAAATCACTAAAGAAAATTGCTCGTGATGCTACTGTCTCAACCACTAAAAGGATTCTAGAAAGAATTTCTGTTCATTATGTGTCACAGAGAATGGCATGGAAACTTCTGAAAGATACCTCAAAGTCTGCGATGAGGAAAGCTGGAAGAAGAATGCCAACTTACGTTTTCTTCTTCAGTGTTAGCAAGATGACTATGAGAGGACACTTTCTAGATGTTGCAGCATCTTGGATCATCTCAGTTAGCATTGATATCTACCGAACTTTTTCTGGAATGACAAACTCTAAAGAAGTGGTTGATGAAATTGATACACCACAAAAGCTTAAACGTCTTCGGAATAAGGTTTGGGGCACTACTTTCAGGTGTGGTGCATCACTGATATTTGCTTCGATTGGAGCAGGTATTGGCGCCACTCTTTTTCGCCCTTCAACTGGCCAGTGGATTGGTCGTATTCTGGGAGATGTGGCCGGGCCTATTATTGTCTCATATTGCCTTGAGAAGTCTTTCCATGCAAAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

329

Amino Acids

36.72

Weight (kDa)

9.56

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 349
Acc36I ACCTGC 1 cut(s) 857
Acc65I GGTACC 1 cut(s) 272
AccB1I GGYRCC 2 cut(s) 272, 875
AciI CCGC 6 cut(s) 5, 87, 99, 105, 120, 424
AclWI GGATC 1 cut(s) 707
AcoI YGGCCR 2 cut(s) 901, 933
AcsI RAATTY 2 cut(s) 265, 534
AcuI CTGAAG 4 cut(s) 136, 225, 453, 628
AcyI GRCGYC 1 cut(s) 876
AfaI GTAC 2 cut(s) 225, 274
AfiI CCNNNNNNNGG 2 cut(s) 519, 899
AflIII ACRYGT 1 cut(s) 445
AgsI TTSAA 2 cut(s) 469, 897
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 5 cut(s) 128, 314, 329, 611, 790
AluI AGCT 5 cut(s) 128, 314, 329, 611, 790
Alw21I GWGCWC 2 cut(s) 130, 331
Alw26I GTCTC 3 cut(s) 28, 511, 955
AlwI GGATC 1 cut(s) 707
AlwNI CAGNNNCTG 1 cut(s) 101
AoxI GGCC 3 cut(s) 901, 933, 938
ApeKI GCWGC 3 cut(s) 95, 101, 689
ApoI RAATTY 2 cut(s) 265, 534
ArsI GACNNNNNNTTYG 2 cut(s) 31, 63
Asp700I GAANNNNTTC 2 cut(s) 733, 971
Asp718I GGTACC 1 cut(s) 272
AspLEI GCGC 1 cut(s) 878
AspS9I GGNCC 1 cut(s) 938
AsuC2I CCSGG 1 cut(s) 937
AsuII TTCGAA 1 cut(s) 318
BaeGI GKGCMC 1 cut(s) 821
BalI TGGCCA 1 cut(s) 903
BanI GGYRCC 2 cut(s) 272, 875
BanII GRGCYC 2 cut(s) 130, 331
BauI CACGAG 1 cut(s) 492
BbsI GAAGAC 1 cut(s) 791
Bbv12I GWGCWC 2 cut(s) 130, 331
BbvI GCAGC 3 cut(s) 88, 107, 701
BciT130I CCWGG 1 cut(s) 387
BcnI CCSGG 1 cut(s) 937
BcoDI GTCTC 3 cut(s) 28, 511, 955
BfaI CTAG 2 cut(s) 527, 680
BfoI RGCGCY 1 cut(s) 879
BfuAI ACCTGC 1 cut(s) 857
BglII AGATCT 1 cut(s) 25
BisI GCNGC 6 cut(s) 96, 99, 102, 105, 120, 690
BlsI GCNGC 6 cut(s) 97, 100, 103, 106, 121, 691
Bme1390I CCNGG 2 cut(s) 387, 937
BmgT120I GGNCC 1 cut(s) 938
BmiI GGNNCC 4 cut(s) 168, 274, 384, 877
BmrFI CCNGG 2 cut(s) 387, 937
BmsI GCATC 5 cut(s) 175, 242, 487, 701, 848
BpiI GAAGAC 1 cut(s) 791
BpmI CTGGAG 3 cut(s) 144, 210, 401
Bpu14I TTCGAA 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 983
BpuMI CCSGG 1 cut(s) 937
BsaHI GRCGYC 1 cut(s) 876
BsaJI CCNNGG 1 cut(s) 276
Bsc4I CCNNNNNNNGG 2 cut(s) 519, 899
Bse1I ACTGG 3 cut(s) 170, 904, 904
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 1 cut(s) 233
BseLI CCNNNNNNNGG 2 cut(s) 519, 899
BseMII CTCAG 4 cut(s) 23, 324, 338, 720
BseNI ACTGG 3 cut(s) 170, 904, 904
BseSI GKGCMC 1 cut(s) 821
BseXI GCAGC 3 cut(s) 88, 107, 701
BshFI GGCC 3 cut(s) 903, 935, 940
BshNI GGYRCC 2 cut(s) 272, 875
BsiHKAI GWGCWC 2 cut(s) 130, 331
BsiSI CCGG 1 cut(s) 936
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 2 cut(s) 519, 899
BsmAI GTCTC 3 cut(s) 28, 511, 955
BsmFI GGGAC 1 cut(s) 440
BsmI GAATGC 1 cut(s) 627
BsnI GGCC 3 cut(s) 903, 935, 940
Bsp119I TTCGAA 1 cut(s) 318
Bsp1286I GDGCHC 3 cut(s) 130, 331, 821
Bsp143I GATC 2 cut(s) 25, 699
BspACI CCGC 6 cut(s) 5, 87, 99, 105, 120, 424
BspANI GGCC 3 cut(s) 903, 935, 940
BspCNI CTCAG 4 cut(s) 24, 323, 339, 719
BspLI GGNNCC 4 cut(s) 168, 274, 384, 877
BspMI ACCTGC 1 cut(s) 857
BspPI GGATC 1 cut(s) 707
BspT104I TTCGAA 1 cut(s) 318
BspT107I GGYRCC 2 cut(s) 272, 875
BsrI ACTGG 3 cut(s) 170, 904, 904
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 2 cut(s) 25, 699
BssNI GRCGYC 1 cut(s) 876
BssSI CACGAG 1 cut(s) 492
BssT1I CCWWGG 1 cut(s) 276
Bst2BI CACGAG 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 1 cut(s) 381
BstACI GRCGYC 1 cut(s) 876
BstAPI GCANNNNNTGC 1 cut(s) 101
BstBI TTCGAA 1 cut(s) 318
BstDEI CTNAG 5 cut(s) 22, 32, 310, 347, 706
BstF5I GGATG 1 cut(s) 233
BstH2I RGCGCY 1 cut(s) 879
BstHHI GCGC 1 cut(s) 878
BstKTI GATC 2 cut(s) 28, 702
BstMAI GTCTC 3 cut(s) 28, 511, 955
BstMBI GATC 2 cut(s) 25, 699
BstMWI GCNNNNNNNGC 6 cut(s) 11, 95, 101, 104, 125, 497
BstNI CCWGG 1 cut(s) 387
BstNSI RCATGY 1 cut(s) 449
BstSCI CCNGG 2 cut(s) 385, 935
BstSLI GKGCMC 1 cut(s) 821
BstV1I GCAGC 3 cut(s) 88, 107, 701
BstV2I GAAGAC 1 cut(s) 791
BstX2I RGATCY 1 cut(s) 25
BstXI CCANNNNNNTGG 1 cut(s) 911
BstYI RGATCY 1 cut(s) 25
BsuRI GGCC 3 cut(s) 903, 935, 940
BtgZI GCGATG 1 cut(s) 614
BtsCI GGATG 1 cut(s) 233
BtsIMutI CAGTG 4 cut(s) 429, 652, 842, 911
BveI ACCTGC 1 cut(s) 857
CaiI CAGNNNCTG 1 cut(s) 101
CfoI GCGC 1 cut(s) 878
Cfr13I GGNCC 1 cut(s) 938
Csp6I GTAC 2 cut(s) 224, 273
CviAII CATG 3 cut(s) 446, 567, 977
CviQI GTAC 2 cut(s) 224, 273
DdeI CTNAG 5 cut(s) 22, 32, 310, 347, 706
DinI GGCGCC 1 cut(s) 877
DpnI GATC 2 cut(s) 27, 701
DpnII GATC 2 cut(s) 25, 699
DrdI GACNNNNNNGTC 1 cut(s) 349
DseDI GACNNNNNNGTC 1 cut(s) 349
EaeI YGGCCR 2 cut(s) 901, 933
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Ecl136II GAGCTC 2 cut(s) 128, 329
Eco130I CCWWGG 1 cut(s) 276
Eco24I GRGCYC 2 cut(s) 130, 331
Eco32I GATATC 1 cut(s) 721
Eco53kI GAGCTC 2 cut(s) 128, 329
Eco57I CTGAAG 4 cut(s) 136, 225, 453, 628
EcoICRI GAGCTC 2 cut(s) 128, 329
EcoRII CCWGG 1 cut(s) 385
EcoRV GATATC 1 cut(s) 721
EcoT14I CCWWGG 1 cut(s) 276
EcoT38I GRGCYC 2 cut(s) 130, 331
EgeI GGCGCC 1 cut(s) 877
EheI GGCGCC 1 cut(s) 877
ErhI CCWWGG 1 cut(s) 276
FaeI CATG 3 cut(s) 449, 570, 980
FaiI YATR 7 cut(s) 447, 456, 549, 568, 665, 955, 978
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 1 cut(s) 440
FatI CATG 3 cut(s) 445, 566, 976
FauI CCCGC 1 cut(s) 417
Fnu4HI GCNGC 6 cut(s) 96, 99, 102, 105, 120, 690
FokI GGATG 1 cut(s) 220
FriOI GRGCYC 2 cut(s) 130, 331
Fsp4HI GCNGC 6 cut(s) 96, 99, 102, 105, 120, 690
FspBI CTAG 2 cut(s) 527, 680
GlaI GCGC 1 cut(s) 877
GluI GCNGC 6 cut(s) 96, 99, 102, 105, 120, 690
GsuI CTGGAG 3 cut(s) 144, 210, 401
HaeII RGCGCY 1 cut(s) 879
HaeIII GGCC 3 cut(s) 903, 935, 940
HapII CCGG 1 cut(s) 936
HhaI GCGC 1 cut(s) 878
Hin1I GRCGYC 1 cut(s) 876
Hin1II CATG 3 cut(s) 449, 570, 980
Hin6I GCGC 1 cut(s) 876
HinP1I GCGC 1 cut(s) 876
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 3 cut(s) 257, 343, 523
HpaII CCGG 1 cut(s) 936
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 6 cut(s) 205, 252, 339, 348, 579, 804
Hpy188III TCNNGA 5 cut(s) 380, 494, 527, 680, 738
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 2 cut(s) 157, 903
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4IV ACGT 3 cut(s) 135, 633, 796
HpyCH4V TGCA 5 cut(s) 233, 400, 689, 839, 980
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 95, 101, 104, 125, 497
HpyF3I CTNAG 5 cut(s) 22, 32, 310, 347, 706
HpySE526I ACGT 3 cut(s) 135, 633, 796
Hsp92I GRCGYC 1 cut(s) 876
Hsp92II CATG 3 cut(s) 449, 570, 980
HspAI GCGC 1 cut(s) 876
KasI GGCGCC 1 cut(s) 875
KpnI GGTACC 1 cut(s) 276
Kzo9I GATC 2 cut(s) 25, 699
LmnI GCTCC 6 cut(s) 125, 133, 191, 382, 466, 863
Lsp1109I GCAGC 3 cut(s) 88, 107, 701
LweI GCATC 5 cut(s) 175, 242, 487, 701, 848
MaeI CTAG 2 cut(s) 527, 680
MaeII ACGT 3 cut(s) 135, 633, 796
MaeIII GTNAC 1 cut(s) 552
MalI GATC 2 cut(s) 27, 701
MboI GATC 2 cut(s) 25, 699
MfeI CAATTG 1 cut(s) 15
MflI RGATCY 1 cut(s) 25
MhlI GDGCHC 3 cut(s) 130, 331, 821
MlsI TGGCCA 1 cut(s) 903
MluCI AATT 7 cut(s) 15, 157, 265, 320, 486, 534, 771
MluNI TGGCCA 1 cut(s) 903
Mly113I GGCGCC 1 cut(s) 876
MlyI GAGTC 1 cut(s) 337
MnlI CCTC 9 cut(s) 121, 226, 312, 342, 384, 397, 597, 598, 662
Mox20I TGGCCA 1 cut(s) 903
MroXI GAANNNNTTC 2 cut(s) 733, 971
MscI TGGCCA 1 cut(s) 903
MseI TTAA 3 cut(s) 239, 296, 792
Msp20I TGGCCA 1 cut(s) 903
MspA1I CMGCKG 2 cut(s) 101, 122
MspI CCGG 1 cut(s) 936
MspR9I CCNGG 2 cut(s) 387, 937
MunI CAATTG 1 cut(s) 15
Mva1269I GAATGC 1 cut(s) 627
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 6 cut(s) 11, 95, 101, 104, 125, 497
NarI GGCGCC 1 cut(s) 876
NciI CCSGG 1 cut(s) 937
NdeII GATC 2 cut(s) 25, 699
NlaIII CATG 3 cut(s) 449, 570, 980
NlaIV GGNNCC 4 cut(s) 168, 274, 384, 877
NmuCI GTSAC 1 cut(s) 552
NspI RCATGY 1 cut(s) 449
NspV TTCGAA 1 cut(s) 318
PciI ACATGT 1 cut(s) 445
PctI GAATGC 1 cut(s) 627
PdmI GAANNNNTTC 2 cut(s) 733, 971
PfeI GAWTC 2 cut(s) 257, 523
PkrI GCNGC 6 cut(s) 97, 100, 103, 106, 121, 691
PleI GAGTC 1 cut(s) 337
PluTI GGCGCC 1 cut(s) 879
PpsI GAGTC 1 cut(s) 337
PscI ACATGT 1 cut(s) 445
Psp124BI GAGCTC 2 cut(s) 130, 331
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 4 cut(s) 168, 274, 384, 877
PspPI GGNCC 1 cut(s) 938
PstNI CAGNNNCTG 1 cut(s) 101
PsuI RGATCY 1 cut(s) 25
RsaI GTAC 2 cut(s) 225, 274
RsaNI GTAC 2 cut(s) 224, 273
SacI GAGCTC 2 cut(s) 130, 331
SaqAI TTAA 3 cut(s) 239, 296, 792
SatI GCNGC 6 cut(s) 96, 99, 102, 105, 120, 690
Sau3AI GATC 2 cut(s) 25, 699
Sau96I GGNCC 1 cut(s) 938
SchI GAGTC 1 cut(s) 337
ScrFI CCNGG 2 cut(s) 387, 937
SduI GDGCHC 3 cut(s) 130, 331, 821
SfaNI GCATC 5 cut(s) 175, 242, 487, 701, 848
SfoI GGCGCC 1 cut(s) 877
SfuI TTCGAA 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 962
SmoI CTYRAG 1 cut(s) 962
Sse9I AATT 7 cut(s) 15, 157, 265, 320, 486, 534, 771
SsiI CCGC 6 cut(s) 5, 87, 99, 105, 120, 424
SspDI GGCGCC 1 cut(s) 875
SspI AATATT 1 cut(s) 246
SspMI CTAG 2 cut(s) 527, 680
SstI GAGCTC 2 cut(s) 130, 331
StyD4I CCNGG 2 cut(s) 385, 935
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 1 cut(s) 505
TaiI ACGT 3 cut(s) 138, 636, 799
TaqI TCGA 3 cut(s) 291, 318, 857
TasI AATT 7 cut(s) 15, 157, 265, 320, 486, 534, 771
TauI GCSGC 3 cut(s) 101, 107, 122
TfiI GAWTC 2 cut(s) 257, 523
Tru1I TTAA 3 cut(s) 239, 296, 792
Tru9I TTAA 3 cut(s) 239, 296, 792
TscAI CASTG 4 cut(s) 436, 652, 849, 911
TseFI GTSAC 1 cut(s) 552
TseI GCWGC 3 cut(s) 95, 101, 689
Tsp45I GTSAC 1 cut(s) 552
TspDTI ATGAA 3 cut(s) 270, 533, 783
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 4 cut(s) 436, 652, 849, 911
XapI RAATTY 2 cut(s) 265, 534
XbaI TCTAGA 2 cut(s) 526, 679
XceI RCATGY 1 cut(s) 449
XmnI GAANNNNTTC 2 cut(s) 733, 971
XspI CTAG 2 cut(s) 527, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.