Rh5CG486800
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005239
Physical Location & Seq
Forward (+)
67875325 .. 67875833
509 bp
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UTR
Exon/CDS
Intron
Rh5CG486800.1

Sequence Viewer

Length: 435 bp
ATGTGGGAACTCATTCATAACAAGCTGCCCTTTGAAGGCATGTCAAATCTGCAAGCAGCATATGCAGCTGCTTTTAAGAATGTGAGGCCTAGTGCAGAAGACCTGGCAGAGGATTTGGCTTTGACAGTAACCTCATGTTGGAAAGAGGATCCAAATGATCGGCCCAACTTTAGCCAAATTATACAGATGCTCATGCATTATCTCTCTACTATTTCACCGTCAAAACCTGCAATCCCCCAACGGATATTCAGATCTGATAATGTTGTACTGCCACCAGAGTCTCCCGGTATTAGTTCTTTGATGGCTACACACAATGAATTGGGCGAAACCCCAAAAGCAGACATGGAAGAGAAGTCCAAAGTTTTTTCTTCTGCTTTAACATGTGTTGTTGATACTCAAATAAACCATAATGCCAAGCCTCTATGTGGTCTGTAG

Protein Analysis

144

Amino Acids

15.94

Weight (kDa)

5.28

Isoelectric Point (pI)

61.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 1 - 64 2e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 235
AclWI GGATC 2 cut(s) 143, 156
AfaI GTAC 1 cut(s) 267
AfiI CCNNNNNNNGG 4 cut(s) 35, 109, 138, 425
AflIII ACRYGT 1 cut(s) 380
AgsI TTSAA 1 cut(s) 35
AjnI CCWGG 1 cut(s) 102
AluBI AGCT 2 cut(s) 25, 68
AluI AGCT 2 cut(s) 25, 68
Alw26I GTCTC 1 cut(s) 285
AlwI GGATC 2 cut(s) 143, 156
AoxI GGCC 2 cut(s) 86, 161
ApeKI GCWGC 4 cut(s) 25, 56, 65, 68
Asp700I GAANNNNTTC 1 cut(s) 12
AspS9I GGNCC 1 cut(s) 162
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 1 cut(s) 207
BamHI GGATCC 1 cut(s) 148
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 4 cut(s) 12, 55, 68, 77
BccI CCATC 1 cut(s) 295
BciT130I CCWGG 1 cut(s) 104
BcnI CCSGG 1 cut(s) 285
BcoDI GTCTC 1 cut(s) 285
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 431
BfuAI ACCTGC 1 cut(s) 235
BglII AGATCT 1 cut(s) 251
BisI GCNGC 4 cut(s) 26, 57, 66, 69
BlsI GCNGC 4 cut(s) 27, 58, 67, 70
Bme1390I CCNGG 2 cut(s) 104, 285
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 1 cut(s) 150
BmrFI CCNGG 2 cut(s) 104, 285
BmsI GCATC 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 105
BpuMI CCSGG 1 cut(s) 285
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 109, 138, 425
BseBI CCWGG 1 cut(s) 104
BseLI CCNNNNNNNGG 4 cut(s) 35, 109, 138, 425
BseXI GCAGC 4 cut(s) 12, 55, 68, 77
BsgI GTGCAG 1 cut(s) 114
BshFI GGCC 2 cut(s) 88, 163
BsiSI CCGG 1 cut(s) 285
BslI CCNNNNNNNGG 4 cut(s) 35, 109, 138, 425
BsmAI GTCTC 1 cut(s) 285
BsnI GGCC 2 cut(s) 88, 163
Bsp143I GATC 3 cut(s) 148, 157, 251
BspANI GGCC 2 cut(s) 88, 163
BspLI GGNNCC 1 cut(s) 150
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 2 cut(s) 143, 156
BssMI GATC 3 cut(s) 148, 157, 251
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 2 cut(s) 127, 219
Bst6I CTCTTC 1 cut(s) 342
BstAPI GCANNNNNTGC 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 54
BstENI CCTNNNNNAGG 1 cut(s) 107
BstKTI GATC 3 cut(s) 151, 160, 254
BstMAI GTCTC 1 cut(s) 285
BstMBI GATC 3 cut(s) 148, 157, 251
BstMWI GCNNNNNNNGC 2 cut(s) 62, 65
BstNI CCWGG 1 cut(s) 104
BstNSI RCATGY 2 cut(s) 43, 384
BstSCI CCNGG 2 cut(s) 102, 283
BstSFI CTRYAG 1 cut(s) 431
BstV1I GCAGC 4 cut(s) 12, 55, 68, 77
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 2 cut(s) 148, 251
BstYI RGATCY 2 cut(s) 148, 251
BsuRI GGCC 2 cut(s) 88, 163
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 54
Cfr13I GGNCC 1 cut(s) 162
Csp6I GTAC 1 cut(s) 266
CviAII CATG 5 cut(s) 40, 135, 193, 343, 381
CviJI RGCY 8 cut(s) 25, 68, 88, 119, 163, 174, 305, 418
CviKI_1 RGCY 8 cut(s) 25, 68, 88, 119, 163, 174, 305, 418
CviQI GTAC 1 cut(s) 266
DpnI GATC 3 cut(s) 150, 159, 253
DpnII GATC 3 cut(s) 148, 157, 251
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Eco147I AGGCCT 1 cut(s) 88
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 102
EcoT22I ATGCAT 1 cut(s) 198
FaeI CATG 5 cut(s) 43, 138, 196, 346, 384
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FatI CATG 5 cut(s) 39, 134, 192, 342, 380
FauNDI CATATG 1 cut(s) 61
Fnu4HI GCNGC 4 cut(s) 26, 57, 66, 69
Fsp4HI GCNGC 4 cut(s) 26, 57, 66, 69
FspBI CTAG 1 cut(s) 90
GluI GCNGC 4 cut(s) 26, 57, 66, 69
HaeIII GGCC 2 cut(s) 88, 163
HapII CCGG 1 cut(s) 285
Hin1II CATG 5 cut(s) 43, 138, 196, 346, 384
HinfI GANTC 1 cut(s) 278
HpaII CCGG 1 cut(s) 285
HphI GGTGA 1 cut(s) 207
Hpy188I TCNGA 2 cut(s) 251, 256
HpyAV CCTTC 1 cut(s) 29
HpyCH4III ACNGT 2 cut(s) 127, 219
HpyCH4V TGCA 5 cut(s) 52, 65, 95, 196, 230
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 65
Hsp92II CATG 5 cut(s) 43, 138, 196, 346, 384
Kzo9I GATC 3 cut(s) 148, 157, 251
LpnPI CCDG 5 cut(s) 89, 116, 240, 288, 298
Lsp1109I GCAGC 4 cut(s) 12, 55, 68, 77
LweI GCATC 1 cut(s) 177
MaeI CTAG 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 127
MalI GATC 3 cut(s) 150, 159, 253
MboI GATC 3 cut(s) 148, 157, 251
MboII GAAGA 3 cut(s) 110, 359, 360
MflI RGATCY 2 cut(s) 148, 251
MluCI AATT 2 cut(s) 177, 317
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 119
MnlI CCTC 5 cut(s) 78, 103, 139, 142, 429
Mph1103I ATGCAT 1 cut(s) 198
MroXI GAANNNNTTC 1 cut(s) 12
MseI TTAA 2 cut(s) 75, 377
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 1 cut(s) 285
MspR9I CCNGG 2 cut(s) 104, 285
MvaI CCWGG 1 cut(s) 104
MwoI GCNNNNNNNGC 2 cut(s) 62, 65
NciI CCSGG 1 cut(s) 285
NdeI CATATG 1 cut(s) 61
NdeII GATC 3 cut(s) 148, 157, 251
NlaIII CATG 5 cut(s) 43, 138, 196, 346, 384
NlaIV GGNNCC 1 cut(s) 150
NsiI ATGCAT 1 cut(s) 198
NspI RCATGY 2 cut(s) 43, 384
PceI AGGCCT 1 cut(s) 88
PciI ACATGT 1 cut(s) 380
PdmI GAANNNNTTC 1 cut(s) 12
PkrI GCNGC 4 cut(s) 27, 58, 67, 70
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PscI ACATGT 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 1 cut(s) 150
PspPI GGNCC 1 cut(s) 162
PsuI RGATCY 2 cut(s) 148, 251
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
SaqAI TTAA 2 cut(s) 75, 377
SatI GCNGC 4 cut(s) 26, 57, 66, 69
Sau3AI GATC 3 cut(s) 148, 157, 251
Sau96I GGNCC 1 cut(s) 162
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 104, 285
SetI ASST 5 cut(s) 27, 70, 105, 134, 229
SfaNI GCATC 1 cut(s) 177
SfcI CTRYAG 1 cut(s) 431
Sse9I AATT 2 cut(s) 177, 317
SseBI AGGCCT 1 cut(s) 88
SspMI CTAG 1 cut(s) 90
StuI AGGCCT 1 cut(s) 88
StyD4I CCNGG 2 cut(s) 102, 283
TaaI ACNGT 2 cut(s) 127, 219
TasI AATT 2 cut(s) 177, 317
TatI WGTACW 1 cut(s) 265
Tru1I TTAA 2 cut(s) 75, 377
Tru9I TTAA 2 cut(s) 75, 377
TseI GCWGC 4 cut(s) 25, 56, 65, 68
TspDTI ATGAA 2 cut(s) 5, 330
TspGWI ACGGA 1 cut(s) 256
XagI CCTNNNNNAGG 1 cut(s) 107
XceI RCATGY 2 cut(s) 43, 384
XmnI GAANNNNTTC 1 cut(s) 12
XspI CTAG 1 cut(s) 90
Zsp2I ATGCAT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.