Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0005239

Physical Location & Seq

Genomic Coordinates Forward (+)

80932416 .. 80934468

Gene Structure 2053 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G053080.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1059 bp

ATGGGATCTGGTAATGGGGTTTATGCAGTTGGGAGTTTTGATTTGGATGCCAAGTGGCTCATCGATCCAAAGCATCTTTTTGTTGGGCCCAAAATCGGGGAGGGTGCTCATGCCAAAGTCTATGAAGGAAAATATAAGAATCAAAATGTTGCTGTGAAAATTGTCAATAGAGGAGAGACCCCAGAAGAGATTGCCAGGAGAGAAGCACGGTTTGCAAGGGAGGTGGCTATGTTGTCCAGAGTGCAGCACAAAAATTTAGTGAAGTTCATTGGTGCCTGCAAAGAACCTGTCATGGTCATAGTGACTGAGCTTCTTCTTGGGGGGACTTTGCGCAAATACTTGTTCAGTATGCGGCCAAGGTGCTTGGACATGCCTGTGGCAGTTGGGTTTGCACTTGATATTGCTCGTGCTACGGAATGCTTACACTCTCATGGAATCATTCACCGGGACCTGAAACCTGAGAACTTGATTTTGACTGCAGACCATAAAACAGTTAAACTTGCGGATTTTGGCTTAGCAAGAGAAGAGTCATTAACAGAAATGATGACTGCTGAAACTGGGACATACCGGTGGATGGCTCCTGAGCTTTATAGCACAGTTACATTAAGACATGGAGAGAAGAAGCATTACAATCATAAGGTGGATGCTTACAGCTTTGCAATTGTATTGTGGGAACTCATTCATAATAAGCTGCCCTTTGAAGGCATGTCAAATTTGCAAGCAGCATATGCAGCTGCTTTTAAGAATGTGAGGCCAAGTGCTGAAGACCTGCCAGAGGATTTGGCTTTGATAGTAACCTCATGTTGGAAAGAGGATCCAAATGATCGGCCCAACTTTAGCCAAATTATACAGATGCTTATGCATTATCTCTCTACTATTTCACCATCAGAACCCGCAATCCCCCAGCGGATATTCAGATCTGATAATGCTGTACTGCCTCCTGAGTCTCCCGGTACTAGTTCTTTGATGGCTGCACACAATGAATCGGGCGAAACCCCTAAAGCAGACATGGAAGAGAAGCCCAAAGGTTTTTTCTTCTGCTTTAACATGTGTTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

352

Amino Acids

39.45

Weight (kDa)

6.68

Isoelectric Point (pI)

49.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	30 - 286	1.2e-55	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	30 - 284	1.7e-50	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005239)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27560 AT5G40540 AT5G40540 AT5G40540
fragaria_vesca	FvH4_6g51650 FvH4_6g51650
malus_domestica	MD09G1024000.v1.1 MD09G1045400.v1.1 MD17G1023300.v1.1
prunus_persica	Prupe.3G293100_v2.0.a1 Prupe.3G293100_v2.0.a1 Prupe.3G293100_v2.0.a1 Prupe.3G293100_v2.0.a1
pyrus_communis	pycom111g01980 pycom17g02000
rosa_chinensis	RchiOBHm_Chr2g0173171
rosa_laevigata	RLG00000022168
rosa_multiflora	Rmu_co8261987.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079140
rosa_rugosa	Rorug02G0566000 Rorug02G0566000
rosa_samantha	Rh2AG646300 Rh2BG657000 Rh2CG622100 Rh2DG670400 Rh5BG464800 Rh5CG486800 Rh6BG252800
rosa_wichuraiana	Rw2G053080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	332
Acc36I	ACCTGC	1 cut(s)	777
AccB1I	GGYRCC	1 cut(s)	272
AciI	CCGC	4 cut(s)	352, 503, 894, 907
AclWI	GGATC	4 cut(s)	13, 59, 809, 822
AcoI	YGGCCR	1 cut(s)	353
AcsI	RAATTY	2 cut(s)	253, 712
AcuI	CTGAAG	1 cut(s)	783
AfaI	GTAC	2 cut(s)	933, 955
AfiI	CCNNNNNNNGG	6 cut(s)	95, 96, 574, 701, 775, 804
AflIII	ACRYGT	1 cut(s)	1047
AgeI	ACCGGT	1 cut(s)	567
AgsI	TTSAA	1 cut(s)	701
AhlI	ACTAGT	1 cut(s)	956
AjnI	CCWGG	1 cut(s)	194
AjuI	GAANNNNNNNTTGG	2 cut(s)	1016, 1048
AloI	GAACNNNNNNTCC	2 cut(s)	882, 914
AluBI	AGCT	5 cut(s)	310, 586, 654, 691, 734
AluI	AGCT	5 cut(s)	310, 586, 654, 691, 734
Alw21I	GWGCWC	1 cut(s)	109
Alw26I	GTCTC	2 cut(s)	170, 951
AlwI	GGATC	4 cut(s)	13, 59, 809, 822
AoxI	GGCC	4 cut(s)	86, 353, 752, 827
ApaI	GGGCCC	1 cut(s)	90
ApeKI	GCWGC	6 cut(s)	244, 691, 722, 731, 734, 971
ApoI	RAATTY	2 cut(s)	253, 712
ArsI	GACNNNNNNTTYG	2 cut(s)	273, 305
AsiGI	ACCGGT	1 cut(s)	567
Asp700I	GAANNNNTTC	1 cut(s)	678
AspLEI	GCGC	1 cut(s)	333
AspS9I	GGNCC	4 cut(s)	86, 87, 448, 828
AsuC2I	CCSGG	2 cut(s)	446, 951
AsuHPI	GGTGA	2 cut(s)	434, 873
AvaII	GGWCC	1 cut(s)	448
BaeGI	GKGCMC	1 cut(s)	90
BamHI	GGATCC	1 cut(s)	814
BanI	GGYRCC	1 cut(s)	272
BanII	GRGCYC	1 cut(s)	90
BarI	GAAGNNNNNNTAC	2 cut(s)	611, 643
BauI	CACGAG	1 cut(s)	405
BbsI	GAAGAC	1 cut(s)	771
Bbv12I	GWGCWC	1 cut(s)	109
BbvI	GCAGC	6 cut(s)	256, 678, 721, 734, 743, 958
BccI	CCATC	3 cut(s)	568, 892, 961
BciT130I	CCWGG	1 cut(s)	196
BcnI	CCSGG	2 cut(s)	446, 951
BcoDI	GTCTC	2 cut(s)	170, 951
BcuI	ACTAGT	1 cut(s)	956
BfaI	CTAG	1 cut(s)	957
BfmI	CTRYAG	1 cut(s)	477
BfuAI	ACCTGC	1 cut(s)	777
BglII	AGATCT	1 cut(s)	917
BisI	GCNGC	7 cut(s)	245, 353, 692, 723, 732, 735, 972
BlpI	GCTNAGC	1 cut(s)	514
BlsI	GCNGC	7 cut(s)	246, 354, 693, 724, 733, 736, 973
Bme1390I	CCNGG	3 cut(s)	196, 446, 951
Bme18I	GGWCC	1 cut(s)	448
BmgT120I	GGNCC	4 cut(s)	86, 87, 448, 828
BmiI	GGNNCC	5 cut(s)	88, 274, 449, 579, 816
BmrFI	CCNGG	3 cut(s)	196, 446, 951
BmrI	ACTGGG	1 cut(s)	567
BmsI	GCATC	4 cut(s)	37, 82, 634, 843
BmuI	ACTGGG	1 cut(s)	567
BpiI	GAAGAC	1 cut(s)	771
Bpu10I	CCTNAGC	1 cut(s)	582
Bpu1102I	GCTNAGC	1 cut(s)	514
BpuMI	CCSGG	2 cut(s)	446, 951
Bsa29I	ATCGAT	1 cut(s)	63
BsaI	GGTCTC	1 cut(s)	170
BsaJI	CCNNGG	1 cut(s)	356
BsaWI	WCCGGW	1 cut(s)	567
Bsc4I	CCNNNNNNNGG	6 cut(s)	95, 96, 574, 701, 775, 804
Bse118I	RCCGGY	1 cut(s)	567
Bse1I	ACTGG	1 cut(s)	562
BseBI	CCWGG	1 cut(s)	196
BseCI	ATCGAT	1 cut(s)	63
BseDI	CCNNGG	1 cut(s)	356
BseGI	GGATG	3 cut(s)	52, 579, 649
BseLI	CCNNNNNNNGG	6 cut(s)	95, 96, 574, 701, 775, 804
BseMII	CTCAG	4 cut(s)	297, 450, 573, 933
BseNI	ACTGG	1 cut(s)	562
BseRI	GAGGAG	1 cut(s)	186
BseSI	GKGCMC	1 cut(s)	90
BseXI	GCAGC	6 cut(s)	256, 678, 721, 734, 743, 958
BseYI	CCCAGC	1 cut(s)	903
BsgI	GTGCAG	2 cut(s)	263, 957
BshFI	GGCC	4 cut(s)	88, 355, 754, 829
BshNI	GGYRCC	1 cut(s)	272
BshTI	ACCGGT	1 cut(s)	567
BshVI	ATCGAT	1 cut(s)	63
BsiHKAI	GWGCWC	1 cut(s)	109
BsiSI	CCGG	3 cut(s)	445, 568, 951
BslFI	GGGAC	3 cut(s)	337, 461, 574
BslI	CCNNNNNNNGG	6 cut(s)	95, 96, 574, 701, 775, 804
BsmAI	GTCTC	2 cut(s)	170, 951
BsmFI	GGGAC	3 cut(s)	337, 461, 574
BsmI	GAATGC	1 cut(s)	422
BsnI	GGCC	4 cut(s)	88, 355, 754, 829
Bso31I	GGTCTC	1 cut(s)	170
Bsp120I	GGGCCC	1 cut(s)	86
Bsp1286I	GDGCHC	2 cut(s)	90, 109
Bsp143I	GATC	5 cut(s)	5, 64, 814, 823, 917
Bsp1720I	GCTNAGC	1 cut(s)	514
BspACI	CCGC	4 cut(s)	352, 503, 894, 907
BspANI	GGCC	4 cut(s)	88, 355, 754, 829
BspCNI	CTCAG	4 cut(s)	298, 451, 574, 934
BspDI	ATCGAT	1 cut(s)	63
BspLI	GGNNCC	5 cut(s)	88, 274, 449, 579, 816
BspMAI	CTGCAG	1 cut(s)	481
BspMI	ACCTGC	1 cut(s)	777
BspPI	GGATC	4 cut(s)	13, 59, 809, 822
BspT107I	GGYRCC	1 cut(s)	272
BspTNI	GGTCTC	1 cut(s)	170
BsrFI	RCCGGY	1 cut(s)	567
BsrI	ACTGG	1 cut(s)	562
BssAI	RCCGGY	1 cut(s)	567
BssECI	CCNNGG	1 cut(s)	356
BssMI	GATC	5 cut(s)	5, 64, 814, 823, 917
BssSI	CACGAG	1 cut(s)	405
BssT1I	CCWWGG	1 cut(s)	356
Bst2BI	CACGAG	1 cut(s)	405
Bst2UI	CCWGG	1 cut(s)	196
Bst4CI	ACNGT	3 cut(s)	210, 493, 598
Bst6I	CTCTTC	3 cut(s)	180, 519, 1008
BstAPI	GCANNNNNTGC	2 cut(s)	212, 728
BstC8I	GCNNGC	2 cut(s)	277, 720
BstDEI	CTNAG	5 cut(s)	306, 459, 514, 582, 942
BstENI	CCTNNNNNAGG	1 cut(s)	773
BstF5I	GGATG	3 cut(s)	52, 579, 649
BstHHI	GCGC	1 cut(s)	333
BstKTI	GATC	5 cut(s)	8, 67, 817, 826, 920
BstMAI	GTCTC	2 cut(s)	170, 951
BstMBI	GATC	5 cut(s)	5, 64, 814, 823, 917
BstMWI	GCNNNNNNNGC	3 cut(s)	212, 728, 731
BstNI	CCWGG	1 cut(s)	196
BstNSI	RCATGY	3 cut(s)	373, 709, 1051
BstSCI	CCNGG	3 cut(s)	194, 444, 949
BstSFI	CTRYAG	1 cut(s)	477
BstSLI	GKGCMC	1 cut(s)	90
BstV1I	GCAGC	6 cut(s)	256, 678, 721, 734, 743, 958
BstV2I	GAAGAC	1 cut(s)	771
BstX2I	RGATCY	3 cut(s)	5, 814, 917
BstYI	RGATCY	3 cut(s)	5, 814, 917
Bsu15I	ATCGAT	1 cut(s)	63
BsuRI	GGCC	4 cut(s)	88, 355, 754, 829
BsuTUI	ATCGAT	1 cut(s)	63
BtsCI	GGATG	3 cut(s)	52, 579, 649
BveI	ACCTGC	1 cut(s)	777
Cac8I	GCNNGC	2 cut(s)	277, 720
CfoI	GCGC	1 cut(s)	333
Cfr10I	RCCGGY	1 cut(s)	567
Cfr13I	GGNCC	4 cut(s)	86, 87, 448, 828
ClaI	ATCGAT	1 cut(s)	63
Csp6I	GTAC	2 cut(s)	932, 954
CspAI	ACCGGT	1 cut(s)	567
CspCI	CAANNNNNGTGG	2 cut(s)	204, 239
CviAII	CATG	9 cut(s)	110, 292, 370, 431, 611, 706, 801, 1009, 1048
CviQI	GTAC	2 cut(s)	932, 954
DdeI	CTNAG	5 cut(s)	306, 459, 514, 582, 942
DpnI	GATC	5 cut(s)	7, 66, 816, 825, 919
DpnII	GATC	5 cut(s)	5, 64, 814, 823, 917
EaeI	YGGCCR	1 cut(s)	353
Eam1104I	CTCTTC	3 cut(s)	180, 519, 1008
EarI	CTCTTC	3 cut(s)	180, 519, 1008
Eco130I	CCWWGG	1 cut(s)	356
Eco24I	GRGCYC	1 cut(s)	90
Eco31I	GGTCTC	1 cut(s)	170
Eco47I	GGWCC	1 cut(s)	448
Eco57I	CTGAAG	1 cut(s)	783
EcoNI	CCTNNNNNAGG	1 cut(s)	773
EcoO109I	RGGNCCY	1 cut(s)	448
EcoRII	CCWGG	1 cut(s)	194
EcoT14I	CCWWGG	1 cut(s)	356
EcoT22I	ATGCAT	1 cut(s)	864
EcoT38I	GRGCYC	1 cut(s)	90
ErhI	CCWWGG	1 cut(s)	356
FaeI	CATG	9 cut(s)	113, 295, 373, 434, 614, 709, 804, 1012, 1051
FalI	AAGNNNNNCTT	2 cut(s)	680, 712
FaqI	GGGAC	3 cut(s)	337, 461, 574
FatI	CATG	9 cut(s)	109, 291, 369, 430, 610, 705, 800, 1008, 1047
FauI	CCCGC	1 cut(s)	901
FauNDI	CATATG	1 cut(s)	727
Fnu4HI	GCNGC	7 cut(s)	245, 353, 692, 723, 732, 735, 972
FokI	GGATG	3 cut(s)	59, 586, 656
FriOI	GRGCYC	1 cut(s)	90
Fsp4HI	GCNGC	7 cut(s)	245, 353, 692, 723, 732, 735, 972
FspBI	CTAG	1 cut(s)	957
FspI	TGCGCA	1 cut(s)	332
GlaI	GCGC	1 cut(s)	332
GluI	GCNGC	7 cut(s)	245, 353, 692, 723, 732, 735, 972
GsaI	CCCAGC	1 cut(s)	907
HaeIII	GGCC	4 cut(s)	88, 355, 754, 829
HapII	CCGG	3 cut(s)	445, 568, 951
HhaI	GCGC	1 cut(s)	333
Hin1II	CATG	9 cut(s)	113, 295, 373, 434, 614, 709, 804, 1012, 1051
Hin6I	GCGC	1 cut(s)	331
HinP1I	GCGC	1 cut(s)	331
HinfI	GANTC	5 cut(s)	139, 435, 527, 944, 983
HpaII	CCGG	3 cut(s)	445, 568, 951
HphI	GGTGA	2 cut(s)	434, 873
Hpy188I	TCNGA	3 cut(s)	889, 917, 922
Hpy188III	TCNNGA	3 cut(s)	237, 581, 941
HpyAV	CCTTC	2 cut(s)	119, 695
HpyCH4III	ACNGT	3 cut(s)	210, 493, 598
HpyF10VI	GCNNNNNNNGC	3 cut(s)	212, 728, 731
HpyF3I	CTNAG	5 cut(s)	306, 459, 514, 582, 942
Hsp92II	CATG	9 cut(s)	113, 295, 373, 434, 614, 709, 804, 1012, 1051
HspAI	GCGC	1 cut(s)	331
Kzo9I	GATC	5 cut(s)	5, 64, 814, 823, 917
LmnI	GCTCC	1 cut(s)	583
Lsp1109I	GCAGC	6 cut(s)	256, 678, 721, 734, 743, 958
LweI	GCATC	4 cut(s)	37, 82, 634, 843
MaeI	CTAG	1 cut(s)	957
MaeIII	GTNAC	3 cut(s)	301, 598, 793
MalI	GATC	5 cut(s)	7, 66, 816, 825, 919
MboI	GATC	5 cut(s)	5, 64, 814, 823, 917
MboII	GAAGA	7 cut(s)	197, 305, 536, 631, 776, 1025, 1027
MfeI	CAATTG	1 cut(s)	660
MflI	RGATCY	3 cut(s)	5, 814, 917
MhlI	GDGCHC	2 cut(s)	90, 109
MluCI	AATT	5 cut(s)	159, 253, 660, 712, 843
MlyI	GAGTC	2 cut(s)	536, 953
MmeI	TCCRAC	1 cut(s)	785
MnlI	CCTC	8 cut(s)	94, 164, 214, 744, 769, 805, 808, 948
Mph1103I	ATGCAT	1 cut(s)	864
MroXI	GAANNNNTTC	1 cut(s)	678
MseI	TTAA	5 cut(s)	495, 533, 605, 741, 1044
MslI	CAYNNNNRTG	3 cut(s)	374, 429, 568
MspA1I	CMGCKG	2 cut(s)	734, 907
MspI	CCGG	3 cut(s)	445, 568, 951
MspR9I	CCNGG	3 cut(s)	196, 446, 951
MunI	CAATTG	1 cut(s)	660
Mva1269I	GAATGC	1 cut(s)	422
MvaI	CCWGG	1 cut(s)	196
MwoI	GCNNNNNNNGC	3 cut(s)	212, 728, 731
NciI	CCSGG	2 cut(s)	446, 951
NdeI	CATATG	1 cut(s)	727
NdeII	GATC	5 cut(s)	5, 64, 814, 823, 917
NlaIII	CATG	9 cut(s)	113, 295, 373, 434, 614, 709, 804, 1012, 1051
NlaIV	GGNNCC	5 cut(s)	88, 274, 449, 579, 816
NmuCI	GTSAC	1 cut(s)	301
NsbI	TGCGCA	1 cut(s)	332
NsiI	ATGCAT	1 cut(s)	864
NspI	RCATGY	3 cut(s)	373, 709, 1051
PciI	ACATGT	1 cut(s)	1047
PctI	GAATGC	1 cut(s)	422
PdmI	GAANNNNTTC	1 cut(s)	678
PfeI	GAWTC	3 cut(s)	139, 435, 983
PinAI	ACCGGT	1 cut(s)	567
PkrI	GCNGC	7 cut(s)	246, 354, 693, 724, 733, 736, 973
PleI	GAGTC	2 cut(s)	535, 952
PpsI	GAGTC	2 cut(s)	535, 952
PpuMI	RGGWCCY	1 cut(s)	448
PscI	ACATGT	1 cut(s)	1047
Psp5II	RGGWCCY	1 cut(s)	448
Psp6I	CCWGG	1 cut(s)	194
PspFI	CCCAGC	1 cut(s)	903
PspGI	CCWGG	1 cut(s)	194
PspN4I	GGNNCC	5 cut(s)	88, 274, 449, 579, 816
PspOMI	GGGCCC	1 cut(s)	86
PspPI	GGNCC	4 cut(s)	86, 87, 448, 828
PspPPI	RGGWCCY	1 cut(s)	448
PstI	CTGCAG	1 cut(s)	481
PsuI	RGATCY	3 cut(s)	5, 814, 917
PvuII	CAGCTG	1 cut(s)	734
RsaI	GTAC	2 cut(s)	933, 955
RsaNI	GTAC	2 cut(s)	932, 954
RseI	CAYNNNNRTG	3 cut(s)	374, 429, 568
SaqAI	TTAA	5 cut(s)	495, 533, 605, 741, 1044
SatI	GCNGC	7 cut(s)	245, 353, 692, 723, 732, 735, 972
Sau3AI	GATC	5 cut(s)	5, 64, 814, 823, 917
Sau96I	GGNCC	4 cut(s)	86, 87, 448, 828
SchI	GAGTC	2 cut(s)	536, 953
ScrFI	CCNGG	3 cut(s)	196, 446, 951
SduI	GDGCHC	2 cut(s)	90, 109
SfaNI	GCATC	4 cut(s)	37, 82, 634, 843
SfcI	CTRYAG	1 cut(s)	477
SinI	GGWCC	1 cut(s)	448
SmiMI	CAYNNNNRTG	3 cut(s)	374, 429, 568
SpeI	ACTAGT	1 cut(s)	956
Sse9I	AATT	5 cut(s)	159, 253, 660, 712, 843
SsiI	CCGC	4 cut(s)	352, 503, 894, 907
SspMI	CTAG	1 cut(s)	957
StyD4I	CCNGG	3 cut(s)	194, 444, 949
StyI	CCWWGG	1 cut(s)	356
TaaI	ACNGT	3 cut(s)	210, 493, 598
TaqI	TCGA	1 cut(s)	63
TasI	AATT	5 cut(s)	159, 253, 660, 712, 843
TatI	WGTACW	1 cut(s)	931
TauI	GCSGC	1 cut(s)	355
TfiI	GAWTC	3 cut(s)	139, 435, 983
Tru1I	TTAA	5 cut(s)	495, 533, 605, 741, 1044
Tru9I	TTAA	5 cut(s)	495, 533, 605, 741, 1044
TseFI	GTSAC	1 cut(s)	301
TseI	GCWGC	6 cut(s)	244, 691, 722, 731, 734, 971
Tsp45I	GTSAC	1 cut(s)	301
TspDTI	ATGAA	4 cut(s)	138, 256, 671, 996
TspGWI	ACGGA	1 cut(s)	428
VpaK11BI	GGWCC	1 cut(s)	448
XagI	CCTNNNNNAGG	1 cut(s)	773
XapI	RAATTY	2 cut(s)	253, 712
XceI	RCATGY	3 cut(s)	373, 709, 1051
XmnI	GAANNNNTTC	1 cut(s)	678
XspI	CTAG	1 cut(s)	957
Zsp2I	ATGCAT	1 cut(s)	864

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.