Rh6BG302100

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0008151
Physical Location & Seq
Reverse (-)
53135544 .. 53180214
44671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG302100.1

Sequence Viewer

Length: 1290 bp
ATGGCGGCGGAGAAACCGTTCAAGAGTATATTGAAATCGCTTGAGAAGCCGGACGGCGGCGAGTTCGGAAAGTACTACAGCTTGCCAGCTCTCAATGATCCTAGGATAAGCTACCGTACTCCATTAAGATCCTTCTTGAGTCTGCAATCTATAACTGCCATGAGTTTCCGGTCTTACAAGTCCCGGTTTAAGTTATTGAATGATGGCACTATTCGATGCTGGAGGGAGGGCAAGCGGCACAATGTACATCTGAAGAGATGGCCCAGGAGCTCGAAAGCGAAATTCGAGTGTGTGTTTAGTGAAGTTGGTGTTGTTAAAGAAGATGGGTTTTCGATGCCTGTGAACCCCAGCAGCCAAGAAACCCTGGGTCATTGTTTCATAGAGTTTAATTTTCCTCAGTCCTCAAGGTATCCAAGGTCTCCTTCCTTGCTTAGTCCCGTAACAGAGGAAGACGAAGAACCTGTTCATAGTGATGAAACTAGTGCAGATGACCATGATGACATAATGCAGTGGAATATGCCTGCAAATGACTGGTTTAGCTGCAATAGTAGTAGTTGGAGCATCTATGGGGTTTGTTCTGTTTCCAAAGGTGAATGTCAATCTGCTCTTTGGGACCAATCTCATGAGACAGATGAATGTGAATCTGCTCTTGAAGCGAGGCAGAGAGTAGTTGCATCTCAGGAATCAAAAACAGGTGCAAACTGGACAATCTCCAAGCTTGTTTTTTATTGTGCCACTGGAATAGCAGCAGGGACAGTTGCTGGACTGCTTGGTCTTGGTGGAAGTTTTATTATGGGCCCACTCTTTTTGGAGATGGATATCCCTCCTCAGGTGTCAAGTGCCACAGCCACCTTCATCATGACATTCTCTTCTTCCATGTCAGTGGATATTGTCCTCCAATTATTTGACTGGAAACATACCAAAGACATTCGCGGGGCTAAGAAATCTAATAGACTTGCGCACATCAAGAAGCTTGAGGAAGAAGTGAAACCTTTAAAGAATGTGTCTCACATCAAGGAGCTTGAGGAAGAAGTGAAGCCTTTAAAGAATCTGTCTCATCCAAACATTGTTGTGAGTAGGACCACCTTGACAAATGGGAAACTTTGGTTCCCACAGCACAAATCTCTTCTCCCCAAGTGCTTTTGGACAGAGTATTGGCTGCCCATTCTTACTGGTGCCAACACCAGGCTAAGCAACATGGCAAGCACCAGGCTTGTGTCCCAAGGATCACAGAGAGTACCTCGACTTGTAGACATCGAGCTGCAAGGTACTCGTCATTGTATGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

429

Amino Acids

48.39

Weight (kDa)

8.24

Isoelectric Point (pI)

58.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TauE PF01925 244 - 304 5.4e-09 Sulfite exporter TauE/SafE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 771
Acc16I TGCGCA 1 cut(s) 960
AccB1I GGYRCC 1 cut(s) 1175
AccI GTMKAC 1 cut(s) 1251
AccII CGCG 1 cut(s) 933
AciI CCGC 5 cut(s) 5, 8, 57, 235, 933
AclWI GGATC 3 cut(s) 92, 123, 1234
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 1 cut(s) 272
AfaI GTAC 5 cut(s) 74, 118, 246, 1239, 1270
AfiI CCNNNNNNNGG 3 cut(s) 56, 829, 1185
AgsI TTSAA 4 cut(s) 22, 34, 199, 653
AhlI ACTAGT 1 cut(s) 479
AjnI CCWGG 4 cut(s) 263, 363, 1184, 1208
AjuI GAANNNNNNNTTGG 2 cut(s) 406, 438
AloI GAACNNNNNNTCC 1 cut(s) 34
AluBI AGCT 9 cut(s) 81, 89, 111, 270, 540, 718, 973, 1021, 1261
AluI AGCT 9 cut(s) 81, 89, 111, 270, 540, 718, 973, 1021, 1261
Alw21I GWGCWC 1 cut(s) 272
Alw26I GTCTC 4 cut(s) 423, 620, 1011, 1059
AlwI GGATC 3 cut(s) 92, 123, 1234
AlwNI CAGNNNCTG 1 cut(s) 761
AoxI GGCC 2 cut(s) 260, 796
ApaI GGGCCC 1 cut(s) 800
ApeKI GCWGC 5 cut(s) 351, 540, 746, 1159, 1261
ApoI RAATTY 1 cut(s) 281
Asp700I GAANNNNTTC 2 cut(s) 17, 462
AspA2I CCTAGG 1 cut(s) 101
AspLEI GCGC 1 cut(s) 961
AspS9I GGNCC 5 cut(s) 261, 613, 796, 797, 1080
AsuC2I CCSGG 1 cut(s) 184
AsuHPI GGTGA 1 cut(s) 602
AvaII GGWCC 2 cut(s) 613, 1080
AvrII CCTAGG 1 cut(s) 101
AxyI CCTNAGG 1 cut(s) 828
BaeGI GKGCMC 1 cut(s) 800
BanI GGYRCC 1 cut(s) 1175
BanII GRGCYC 2 cut(s) 272, 800
BbsI GAAGAC 1 cut(s) 456
Bbv12I GWGCWC 1 cut(s) 272
BbvI GCAGC 5 cut(s) 363, 527, 758, 1146, 1248
BccI CCATC 4 cut(s) 197, 252, 317, 808
BceAI ACGGC 1 cut(s) 70
BcgI CGANNNNNNTGC 2 cut(s) 1253, 1287
BciT130I CCWGG 4 cut(s) 265, 365, 1186, 1210
BciVI GTATCC 1 cut(s) 420
BcnI CCSGG 1 cut(s) 184
BcoDI GTCTC 4 cut(s) 423, 620, 1011, 1059
BcuI ACTAGT 1 cut(s) 479
BfaI CTAG 2 cut(s) 102, 480
BfmI CTRYAG 1 cut(s) 76
BfuI GTATCC 1 cut(s) 420
BisI GCNGC 8 cut(s) 6, 58, 236, 352, 541, 747, 1160, 1262
BlnI CCTAGG 1 cut(s) 101
BlpI GCTNAGC 1 cut(s) 1190
BlsI GCNGC 8 cut(s) 7, 59, 237, 353, 542, 748, 1161, 1263
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 5 cut(s) 184, 265, 365, 1186, 1210
Bme18I GGWCC 2 cut(s) 613, 1080
BmgT120I GGNCC 5 cut(s) 261, 613, 796, 797, 1080
BmiI GGNNCC 4 cut(s) 614, 798, 1109, 1177
BmrFI CCNGG 5 cut(s) 184, 265, 365, 1186, 1210
BmsI GCATC 4 cut(s) 206, 324, 570, 683
BpiI GAAGAC 1 cut(s) 456
BplI GAGNNNNNCTC 2 cut(s) 1225, 1257
BpmI CTGGAG 1 cut(s) 241
Bpu1102I GCTNAGC 1 cut(s) 1190
BpuEI CTTGAG 5 cut(s) 62, 157, 388, 995, 1043
BpuMI CCSGG 1 cut(s) 184
BsaBI GATNNNNATC 1 cut(s) 818
BsaI GGTCTC 1 cut(s) 423
BsaJI CCNNGG 6 cut(s) 101, 263, 363, 364, 413, 1222
BsaWI WCCGGW 1 cut(s) 168
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 829, 1185
Bse1I ACTGG 5 cut(s) 536, 707, 742, 914, 1177
Bse21I CCTNAGG 1 cut(s) 828
Bse8I GATNNNNATC 1 cut(s) 818
BseBI CCWGG 4 cut(s) 265, 365, 1186, 1210
BseDI CCNNGG 6 cut(s) 101, 263, 363, 364, 413, 1222
BseGI GGATG 1 cut(s) 1057
BseJI GATNNNNATC 1 cut(s) 818
BseLI CCNNNNNNNGG 3 cut(s) 56, 829, 1185
BseMII CTCAG 3 cut(s) 410, 692, 842
BseNI ACTGG 5 cut(s) 536, 707, 742, 914, 1177
BseRI GAGGAG 1 cut(s) 816
BseSI GKGCMC 1 cut(s) 800
BseXI GCAGC 5 cut(s) 363, 527, 758, 1146, 1248
BseYI CCCAGC 1 cut(s) 347
BsgI GTGCAG 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 933
BshFI GGCC 2 cut(s) 262, 798
BshNI GGYRCC 1 cut(s) 1175
BsiHKAI GWGCWC 1 cut(s) 272
BsiSI CCGG 3 cut(s) 50, 169, 184
BslFI GGGAC 5 cut(s) 166, 420, 626, 766, 1204
BslI CCNNNNNNNGG 3 cut(s) 56, 829, 1185
BsmAI GTCTC 4 cut(s) 423, 620, 1011, 1059
BsmFI GGGAC 5 cut(s) 166, 420, 626, 766, 1204
BsnI GGCC 2 cut(s) 262, 798
Bso31I GGTCTC 1 cut(s) 423
Bsp120I GGGCCC 1 cut(s) 796
Bsp1286I GDGCHC 2 cut(s) 272, 800
Bsp1407I TGTACA 1 cut(s) 244
Bsp143I GATC 3 cut(s) 97, 128, 1226
Bsp1720I GCTNAGC 1 cut(s) 1190
BspACI CCGC 5 cut(s) 5, 8, 57, 235, 933
BspANI GGCC 2 cut(s) 262, 798
BspCNI CTCAG 3 cut(s) 409, 691, 841
BspFNI CGCG 1 cut(s) 933
BspHI TCATGA 2 cut(s) 622, 858
BspLI GGNNCC 4 cut(s) 614, 798, 1109, 1177
BspPI GGATC 3 cut(s) 92, 123, 1234
BspT107I GGYRCC 1 cut(s) 1175
BspTNI GGTCTC 1 cut(s) 423
BsrGI TGTACA 1 cut(s) 244
BsrI ACTGG 5 cut(s) 536, 707, 742, 914, 1177
BssECI CCNNGG 6 cut(s) 101, 263, 363, 364, 413, 1222
BssMI GATC 3 cut(s) 97, 128, 1226
BssT1I CCWWGG 3 cut(s) 101, 413, 1222
Bst2UI CCWGG 4 cut(s) 265, 365, 1186, 1210
Bst4CI ACNGT 3 cut(s) 18, 116, 757
Bst6I CTCTTC 3 cut(s) 248, 874, 1131
BstAUI TGTACA 1 cut(s) 244
BstC8I GCNNGC 5 cut(s) 83, 87, 233, 522, 1204
BstDEI CTNAG 6 cut(s) 396, 431, 678, 828, 939, 1190
BstF5I GGATG 1 cut(s) 1057
BstFNI CGCG 1 cut(s) 933
BstHHI GCGC 1 cut(s) 961
BstKTI GATC 3 cut(s) 100, 131, 1229
BstMAI GTCTC 4 cut(s) 423, 620, 1011, 1059
BstMBI GATC 3 cut(s) 97, 128, 1226
BstMWI GCNNNNNNNGC 2 cut(s) 46, 653
BstNI CCWGG 4 cut(s) 265, 365, 1186, 1210
BstSCI CCNGG 5 cut(s) 182, 263, 363, 1184, 1208
BstSFI CTRYAG 1 cut(s) 76
BstSLI GKGCMC 1 cut(s) 800
BstUI CGCG 1 cut(s) 933
BstV1I GCAGC 5 cut(s) 363, 527, 758, 1146, 1248
BstV2I GAAGAC 1 cut(s) 456
BstX2I RGATCY 1 cut(s) 128
BstXI CCANNNNNNTGG 1 cut(s) 883
BstYI RGATCY 1 cut(s) 128
Bsu36I CCTNAGG 1 cut(s) 828
BsuI GTATCC 1 cut(s) 420
BsuRI GGCC 2 cut(s) 262, 798
BtsCI GGATG 1 cut(s) 1057
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 3 cut(s) 515, 735, 888
Cac8I GCNNGC 5 cut(s) 83, 87, 233, 522, 1204
CaiI CAGNNNCTG 1 cut(s) 761
CciI TCATGA 2 cut(s) 622, 858
CfoI GCGC 1 cut(s) 961
Cfr13I GGNCC 5 cut(s) 261, 613, 796, 797, 1080
Csp6I GTAC 5 cut(s) 73, 117, 245, 1238, 1269
CspCI CAANNNNNGTGG 2 cut(s) 789, 824
CviAII CATG 6 cut(s) 160, 494, 623, 859, 877, 1198
CviQI GTAC 5 cut(s) 73, 117, 245, 1238, 1269
DdeI CTNAG 6 cut(s) 396, 431, 678, 828, 939, 1190
DpnI GATC 3 cut(s) 99, 130, 1228
DpnII GATC 3 cut(s) 97, 128, 1226
DraI TTTAAA 2 cut(s) 996, 1044
DrdI GACNNNNNNGTC 1 cut(s) 771
DseDI GACNNNNNNGTC 1 cut(s) 771
Eam1104I CTCTTC 3 cut(s) 248, 874, 1131
EarI CTCTTC 3 cut(s) 248, 874, 1131
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 270
Eco130I CCWWGG 3 cut(s) 101, 413, 1222
Eco24I GRGCYC 2 cut(s) 272, 800
Eco31I GGTCTC 1 cut(s) 423
Eco32I GATATC 1 cut(s) 820
Eco47I GGWCC 2 cut(s) 613, 1080
Eco53kI GAGCTC 1 cut(s) 270
Eco57I CTGAAG 1 cut(s) 272
Eco81I CCTNAGG 1 cut(s) 828
EcoICRI GAGCTC 1 cut(s) 270
EcoRII CCWGG 4 cut(s) 263, 363, 1184, 1208
EcoRV GATATC 1 cut(s) 820
EcoT14I CCWWGG 3 cut(s) 101, 413, 1222
EcoT38I GRGCYC 2 cut(s) 272, 800
ErhI CCWWGG 3 cut(s) 101, 413, 1222
FaeI CATG 6 cut(s) 163, 497, 626, 862, 880, 1201
FalI AAGNNNNNCTT 2 cut(s) 406, 438
FaqI GGGAC 5 cut(s) 166, 420, 626, 766, 1204
FatI CATG 6 cut(s) 159, 493, 622, 858, 876, 1197
FauI CCCGC 1 cut(s) 926
FblI GTMKAC 1 cut(s) 1251
Fnu4HI GCNGC 8 cut(s) 6, 58, 236, 352, 541, 747, 1160, 1262
FokI GGATG 1 cut(s) 1044
FriOI GRGCYC 2 cut(s) 272, 800
Fsp4HI GCNGC 8 cut(s) 6, 58, 236, 352, 541, 747, 1160, 1262
FspBI CTAG 2 cut(s) 102, 480
FspI TGCGCA 1 cut(s) 960
GlaI GCGC 1 cut(s) 960
GluI GCNGC 8 cut(s) 6, 58, 236, 352, 541, 747, 1160, 1262
GsaI CCCAGC 1 cut(s) 351
GsuI CTGGAG 1 cut(s) 241
HaeIII GGCC 2 cut(s) 262, 798
HapII CCGG 3 cut(s) 50, 169, 184
HhaI GCGC 1 cut(s) 961
Hin1II CATG 6 cut(s) 163, 497, 626, 862, 880, 1201
Hin6I GCGC 1 cut(s) 959
HinP1I GCGC 1 cut(s) 959
HindIII AAGCTT 2 cut(s) 716, 971
HinfI GANTC 4 cut(s) 139, 641, 683, 1048
HpaII CCGG 3 cut(s) 50, 169, 184
HphI GGTGA 1 cut(s) 602
Hpy166II GTNNAC 2 cut(s) 343, 1252
Hpy188I TCNGA 2 cut(s) 68, 252
Hpy188III TCNNGA 7 cut(s) 22, 136, 623, 650, 680, 859, 967
Hpy8I GTNNAC 2 cut(s) 343, 1252
HpyAV CCTTC 3 cut(s) 142, 432, 862
HpyCH4III ACNGT 3 cut(s) 18, 116, 757
HpyCH4V TGCA 8 cut(s) 145, 485, 508, 524, 543, 674, 698, 1264
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 653
HpyF3I CTNAG 6 cut(s) 396, 431, 678, 828, 939, 1190
Hsp92II CATG 6 cut(s) 163, 497, 626, 862, 880, 1201
HspAI GCGC 1 cut(s) 959
Kzo9I GATC 3 cut(s) 97, 128, 1226
LmnI GCTCC 3 cut(s) 267, 558, 1018
Lsp1109I GCAGC 5 cut(s) 363, 527, 758, 1146, 1248
LweI GCATC 4 cut(s) 206, 324, 570, 683
MaeI CTAG 2 cut(s) 102, 480
MaeIII GTNAC 1 cut(s) 439
MalI GATC 3 cut(s) 99, 130, 1228
MboI GATC 3 cut(s) 97, 128, 1226
MboII GAAGA 9 cut(s) 265, 332, 461, 467, 861, 864, 992, 1040, 1118
MflI RGATCY 1 cut(s) 128
MhlI GDGCHC 2 cut(s) 272, 800
MluCI AATT 3 cut(s) 281, 388, 899
MlyI GAGTC 1 cut(s) 148
MmeI TCCRAC 1 cut(s) 536
MroXI GAANNNNTTC 2 cut(s) 17, 462
MseI TTAA 6 cut(s) 125, 189, 315, 387, 995, 1043
MslI CAYNNNNRTG 3 cut(s) 471, 881, 1070
MspI CCGG 3 cut(s) 50, 169, 184
MspR9I CCNGG 5 cut(s) 184, 265, 365, 1186, 1210
MvaI CCWGG 4 cut(s) 265, 365, 1186, 1210
MvnI CGCG 1 cut(s) 933
MwoI GCNNNNNNNGC 2 cut(s) 46, 653
NciI CCSGG 1 cut(s) 184
NdeII GATC 3 cut(s) 97, 128, 1226
NlaIII CATG 6 cut(s) 163, 497, 626, 862, 880, 1201
NlaIV GGNNCC 4 cut(s) 614, 798, 1109, 1177
NsbI TGCGCA 1 cut(s) 960
PagI TCATGA 2 cut(s) 622, 858
PasI CCCWGGG 1 cut(s) 364
PdmI GAANNNNTTC 2 cut(s) 17, 462
PfeI GAWTC 3 cut(s) 641, 683, 1048
PkrI GCNGC 8 cut(s) 7, 59, 237, 353, 542, 748, 1161, 1263
PleI GAGTC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 147
Psp124BI GAGCTC 1 cut(s) 272
Psp6I CCWGG 4 cut(s) 263, 363, 1184, 1208
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 4 cut(s) 263, 363, 1184, 1208
PspN4I GGNNCC 4 cut(s) 614, 798, 1109, 1177
PspOMI GGGCCC 1 cut(s) 796
PspPI GGNCC 5 cut(s) 261, 613, 796, 797, 1080
PstNI CAGNNNCTG 1 cut(s) 761
PsuI RGATCY 1 cut(s) 128
RsaI GTAC 5 cut(s) 74, 118, 246, 1239, 1270
RsaNI GTAC 5 cut(s) 73, 117, 245, 1238, 1269
RseI CAYNNNNRTG 3 cut(s) 471, 881, 1070
SacI GAGCTC 1 cut(s) 272
SaqAI TTAA 6 cut(s) 125, 189, 315, 387, 995, 1043
SatI GCNGC 8 cut(s) 6, 58, 236, 352, 541, 747, 1160, 1262
Sau3AI GATC 3 cut(s) 97, 128, 1226
Sau96I GGNCC 5 cut(s) 261, 613, 796, 797, 1080
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 1 cut(s) 148
ScrFI CCNGG 5 cut(s) 184, 265, 365, 1186, 1210
SduI GDGCHC 2 cut(s) 272, 800
SfaNI GCATC 4 cut(s) 206, 324, 570, 683
SfcI CTRYAG 1 cut(s) 76
SinI GGWCC 2 cut(s) 613, 1080
SmiMI CAYNNNNRTG 3 cut(s) 471, 881, 1070
SmlI CTYRAG 5 cut(s) 41, 136, 403, 974, 1022
SmoI CTYRAG 5 cut(s) 41, 136, 403, 974, 1022
SpeI ACTAGT 1 cut(s) 479
Sse9I AATT 3 cut(s) 281, 388, 899
SsiI CCGC 5 cut(s) 5, 8, 57, 235, 933
SspMI CTAG 2 cut(s) 102, 480
SstI GAGCTC 1 cut(s) 272
StyD4I CCNGG 5 cut(s) 182, 263, 363, 1184, 1208
StyI CCWWGG 3 cut(s) 101, 413, 1222
TaaI ACNGT 3 cut(s) 18, 116, 757
TaqI TCGA 6 cut(s) 214, 272, 285, 332, 1243, 1257
TasI AATT 3 cut(s) 281, 388, 899
TatI WGTACW 2 cut(s) 72, 244
TauI GCSGC 3 cut(s) 8, 60, 238
TfiI GAWTC 3 cut(s) 641, 683, 1048
Tru1I TTAA 6 cut(s) 125, 189, 315, 387, 995, 1043
Tru9I TTAA 6 cut(s) 125, 189, 315, 387, 995, 1043
TscAI CASTG 3 cut(s) 515, 742, 888
TseI GCWGC 5 cut(s) 351, 540, 746, 1159, 1261
TspDTI ATGAA 5 cut(s) 367, 455, 489, 648, 844
TspRI CASTG 3 cut(s) 515, 742, 888
VpaK11BI GGWCC 2 cut(s) 613, 1080
XapI RAATTY 1 cut(s) 281
XmaJI CCTAGG 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 1251
XmnI GAANNNNTTC 2 cut(s) 17, 462
XspI CTAG 2 cut(s) 102, 480
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.