Rh7AG105400

FIST_C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0010565
Physical Location & Seq
Forward (+)
8365217 .. 8367703
2487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG105400.1

Sequence Viewer

Length: 1113 bp
ATGCAGGTGAAGTGGGAATTGGAAAATGGGCCCAACTATTGGGATGAAGAAATTCTAATGGGAGGTAATCCAATAAATGGAGGGTTAATTCTCTCTGTTGGATTTGTGCCCGATTTAAAAGTGGATGTCATCCCACTTCCAAGATCAAAGACTCAACCCAATATGCCATTGATGGAGTACTTTATGCTGGAGCTTAGGAGTTACACTGCTTCCGTTTCAGGTTCTCTCGACCCAGCTTGTATCACAATGTTCGGAGATCATCGTGTTGATATAAGACCCATTGTTGAGAAACTAGATCTTGTCATGCCTGAGGAGACTGCCGTTGTAGGAGACGCAAAAGGCTCCTTTCTGTTCCGCCGCAGGGACCAGACCCAAAATTACGGTGCTGATCTGTTCTTCTTTGATGCTGCTGCTCTAGTATTTGCCAGGGACCAAAACAATTCTGCTGAGTTGGGGGAAACTCGGTTCCATGTGGCGGTGGCCGACGGCATAATTCCATTTGGTCCTGAGCTCAGGAGCGTTTCAGTGTTTGAGGATTTCTTTGCTCAATCTTGGATTTCGGGCGTAGTGGAAGGAGCAGAAGAAGGGTATCCCATGCTTGATTGCGTCACTGTTTTACAACAGCTTATAAATCAGTTGGACTTGGGAAACCCAAATTTATACATTGAGGTCAGGAAAGGTAGACAATACTCTACACAATTCAGAACAACCCTGGCTTGTCATGAACTTTTGGGGGGTAACTACGAGTCCATAATCGTCAAGGGTTGTGGCATACAACCTGGGGATAGATTCATATTCCTCCACTCAGACGCCGGAAGTGCACTGACTCGTGTTGACGATGCTCGTCACAGTTTGGAAGCATTGGATGCTAATCAAGAGGTGTTTAAAGGCTTGTTCTTCTCCAGCTACCATCGCGACAGGTCCTTTTTCGGAGCCTCTCATGTGGATATAACTCCCTTTACTGACAAGCTCAGTGGAGTCCCCATGGCTGGTATCTCCTGCACAAGAGAAATTGGGCGTGGGGCGTTACTTGGTCCAGGACTAGGGTATTGCGTCCATGCACATACCTGTGTGTACCTAGCTATGTCTTATGTTCCTCCAATGTCCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FIST_C

Protein Analysis

370

Amino Acids

40.96

Weight (kDa)

4.9

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 629
AccB7I CCANNNNNTGG 2 cut(s) 39, 77
AccI GTMKAC 1 cut(s) 682
AccII CGCG 1 cut(s) 915
AciI CCGC 3 cut(s) 355, 358, 476
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 2 cut(s) 51, 655
AcyI GRCGYC 1 cut(s) 810
AfaI GTAC 2 cut(s) 179, 1076
AfiI CCNNNNNNNGG 6 cut(s) 39, 77, 361, 475, 989, 1043
AjnI CCWGG 4 cut(s) 425, 711, 778, 1036
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AloI GAACNNNNNNTCC 2 cut(s) 449, 481
AluBI AGCT 7 cut(s) 193, 236, 511, 625, 906, 970, 1082
AluI AGCT 7 cut(s) 193, 236, 511, 625, 906, 970, 1082
Alw21I GWGCWC 2 cut(s) 513, 823
Alw26I GTCTC 2 cut(s) 308, 324
Alw44I GTGCAC 1 cut(s) 819
AoxI GGCC 2 cut(s) 29, 480
ApaI GGGCCC 1 cut(s) 33
ApaLI GTGCAC 1 cut(s) 819
ApeKI GCWGC 2 cut(s) 407, 410
ApoI RAATTY 2 cut(s) 51, 655
Asp700I GAANNNNTTC 1 cut(s) 51
AspS9I GGNCC 7 cut(s) 29, 30, 364, 430, 503, 921, 1034
AsuHPI GGTGA 1 cut(s) 19
AvaII GGWCC 5 cut(s) 364, 430, 503, 921, 1034
AxyI CCTNAGG 1 cut(s) 309
BaeGI GKGCMC 3 cut(s) 33, 111, 823
BanII GRGCYC 2 cut(s) 33, 513
BauI CACGAG 1 cut(s) 828
Bbv12I GWGCWC 2 cut(s) 513, 823
BbvI GCAGC 2 cut(s) 394, 397
BccI CCATC 2 cut(s) 166, 918
BceAI ACGGC 2 cut(s) 305, 502
BciT130I CCWGG 4 cut(s) 427, 713, 780, 1038
BciVI GTATCC 1 cut(s) 600
BcoDI GTCTC 2 cut(s) 308, 324
BfaI CTAG 4 cut(s) 293, 416, 1043, 1079
BfuI GTATCC 1 cut(s) 600
BglII AGATCT 1 cut(s) 295
BisI GCNGC 3 cut(s) 358, 408, 411
BlsI GCNGC 3 cut(s) 359, 409, 412
BmcAI AGTACT 1 cut(s) 179
Bme1390I CCNGG 4 cut(s) 427, 713, 780, 1038
Bme18I GGWCC 5 cut(s) 364, 430, 503, 921, 1034
BmgT120I GGNCC 7 cut(s) 29, 30, 364, 430, 503, 921, 1034
BmiI GGNNCC 6 cut(s) 31, 343, 365, 431, 467, 934
BmrFI CCNGG 4 cut(s) 427, 713, 780, 1038
BmsI GCATC 3 cut(s) 394, 829, 856
BpmI CTGGAG 2 cut(s) 209, 886
Bpu10I CCTNAGC 3 cut(s) 194, 507, 512
BsaBI GATNNNNATC 1 cut(s) 870
BsaHI GRCGYC 1 cut(s) 810
BsaJI CCNNGG 4 cut(s) 426, 711, 779, 984
Bsc4I CCNNNNNNNGG 6 cut(s) 39, 77, 361, 475, 989, 1043
Bse21I CCTNAGG 1 cut(s) 309
Bse8I GATNNNNATC 1 cut(s) 870
BseBI CCWGG 4 cut(s) 427, 713, 780, 1038
BseDI CCNNGG 4 cut(s) 426, 711, 779, 984
BseGI GGATG 4 cut(s) 49, 129, 130, 871
BseJI GATNNNNATC 1 cut(s) 870
BseLI CCNNNNNNNGG 6 cut(s) 39, 77, 361, 475, 989, 1043
BseMII CTCAG 6 cut(s) 300, 438, 498, 526, 819, 985
BseRI GAGGAG 1 cut(s) 326
BseSI GKGCMC 3 cut(s) 33, 111, 823
BseXI GCAGC 2 cut(s) 394, 397
BseYI CCCAGC 1 cut(s) 232
BsgI GTGCAG 1 cut(s) 985
Bsh1236I CGCG 1 cut(s) 915
BshFI GGCC 2 cut(s) 31, 482
BsiHKAI GWGCWC 2 cut(s) 513, 823
BsiSI CCGG 1 cut(s) 813
BslFI GGGAC 3 cut(s) 377, 443, 965
BslI CCNNNNNNNGG 6 cut(s) 39, 77, 361, 475, 989, 1043
BsmAI GTCTC 2 cut(s) 308, 324
BsmBI CGTCTC 1 cut(s) 324
BsmFI GGGAC 3 cut(s) 377, 443, 965
BsnI GGCC 2 cut(s) 31, 482
Bsp120I GGGCCC 1 cut(s) 29
Bsp1286I GDGCHC 4 cut(s) 33, 111, 513, 823
Bsp143I GATC 4 cut(s) 143, 256, 295, 388
Bsp19I CCATGG 1 cut(s) 984
Bsp68I TCGCGA 1 cut(s) 915
BspACI CCGC 3 cut(s) 355, 358, 476
BspANI GGCC 2 cut(s) 31, 482
BspCNI CTCAG 6 cut(s) 301, 439, 499, 525, 818, 984
BspFNI CGCG 1 cut(s) 915
BspHI TCATGA 1 cut(s) 721
BspLI GGNNCC 6 cut(s) 31, 343, 365, 431, 467, 934
BssECI CCNNGG 4 cut(s) 426, 711, 779, 984
BssMI GATC 4 cut(s) 143, 256, 295, 388
BssNI GRCGYC 1 cut(s) 810
BssSI CACGAG 1 cut(s) 828
BssT1I CCWWGG 1 cut(s) 984
Bst2BI CACGAG 1 cut(s) 828
Bst2UI CCWGG 4 cut(s) 427, 713, 780, 1038
Bst4CI ACNGT 3 cut(s) 383, 613, 851
BstACI GRCGYC 1 cut(s) 810
BstAPI GCANNNNNTGC 1 cut(s) 866
BstDEI CTNAG 7 cut(s) 194, 309, 447, 507, 512, 805, 971
BstDSI CCRYGG 1 cut(s) 984
BstF5I GGATG 4 cut(s) 49, 129, 130, 871
BstFNI CGCG 1 cut(s) 915
BstKTI GATC 4 cut(s) 146, 259, 298, 391
BstMAI GTCTC 2 cut(s) 308, 324
BstMBI GATC 4 cut(s) 143, 256, 295, 388
BstMWI GCNNNNNNNGC 3 cut(s) 818, 866, 912
BstNI CCWGG 4 cut(s) 427, 713, 780, 1038
BstSCI CCNGG 4 cut(s) 425, 711, 778, 1036
BstSLI GKGCMC 3 cut(s) 33, 111, 823
BstUI CGCG 1 cut(s) 915
BstV1I GCAGC 2 cut(s) 394, 397
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
Bsu36I CCTNAGG 1 cut(s) 309
BsuI GTATCC 1 cut(s) 600
BsuRI GGCC 2 cut(s) 31, 482
BtgI CCRYGG 1 cut(s) 984
BtgZI GCGATG 1 cut(s) 896
BtsCI GGATG 4 cut(s) 49, 129, 130, 871
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 5 cut(s) 204, 531, 609, 821, 979
BtuMI TCGCGA 1 cut(s) 915
CciI TCATGA 1 cut(s) 721
Cfr13I GGNCC 7 cut(s) 29, 30, 364, 430, 503, 921, 1034
CseI GACGC 4 cut(s) 341, 595, 818, 1042
Csp6I GTAC 2 cut(s) 178, 1075
CspCI CAANNNNNGTGG 4 cut(s) 748, 783, 955, 990
CviAII CATG 7 cut(s) 304, 470, 595, 722, 941, 985, 1058
CviQI GTAC 2 cut(s) 178, 1075
DdeI CTNAG 7 cut(s) 194, 309, 447, 507, 512, 805, 971
DpnI GATC 4 cut(s) 145, 258, 297, 390
DpnII GATC 4 cut(s) 143, 256, 295, 388
DraI TTTAAA 2 cut(s) 117, 886
EaeI YGGCCR 1 cut(s) 480
EciI GGCGGA 1 cut(s) 344
Ecl136II GAGCTC 1 cut(s) 511
Eco130I CCWWGG 1 cut(s) 984
Eco24I GRGCYC 2 cut(s) 33, 513
Eco47I GGWCC 5 cut(s) 364, 430, 503, 921, 1034
Eco53kI GAGCTC 1 cut(s) 511
Eco81I CCTNAGG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 511
EcoO109I RGGNCCY 1 cut(s) 921
EcoRII CCWGG 4 cut(s) 425, 711, 778, 1036
EcoT14I CCWWGG 1 cut(s) 984
EcoT38I GRGCYC 2 cut(s) 33, 513
ErhI CCWWGG 1 cut(s) 984
Esp3I CGTCTC 1 cut(s) 324
FaeI CATG 7 cut(s) 307, 473, 598, 725, 944, 988, 1061
FaqI GGGAC 3 cut(s) 377, 443, 965
FatI CATG 7 cut(s) 303, 469, 594, 721, 940, 984, 1057
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 3 cut(s) 358, 408, 411
FokI GGATG 4 cut(s) 56, 116, 137, 878
FriOI GRGCYC 2 cut(s) 33, 513
Fsp4HI GCNGC 3 cut(s) 358, 408, 411
FspBI CTAG 4 cut(s) 293, 416, 1043, 1079
GluI GCNGC 3 cut(s) 358, 408, 411
GsaI CCCAGC 1 cut(s) 236
GsuI CTGGAG 2 cut(s) 209, 886
HaeIII GGCC 2 cut(s) 31, 482
HapII CCGG 1 cut(s) 813
HgaI GACGC 4 cut(s) 341, 595, 818, 1042
Hin1I GRCGYC 1 cut(s) 810
Hin1II CATG 7 cut(s) 307, 473, 598, 725, 944, 988, 1061
HincII GTYRAC 1 cut(s) 835
HindII GTYRAC 1 cut(s) 835
HinfI GANTC 5 cut(s) 151, 746, 789, 826, 978
HpaII CCGG 1 cut(s) 813
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 4 cut(s) 683, 821, 835, 1075
Hpy188I TCNGA 4 cut(s) 254, 704, 808, 932
Hpy188III TCNNGA 7 cut(s) 227, 506, 514, 673, 722, 875, 914
Hpy8I GTNNAC 4 cut(s) 683, 821, 835, 1075
Hpy99I CGWCG 1 cut(s) 488
HpyAV CCTTC 2 cut(s) 566, 578
HpyCH4III ACNGT 3 cut(s) 383, 613, 851
HpyCH4V TGCA 4 cut(s) 4, 821, 1002, 1061
HpyF10VI GCNNNNNNNGC 3 cut(s) 818, 866, 912
HpyF3I CTNAG 7 cut(s) 194, 309, 447, 507, 512, 805, 971
Hsp92I GRCGYC 1 cut(s) 810
Hsp92II CATG 7 cut(s) 307, 473, 598, 725, 944, 988, 1061
Kzo9I GATC 4 cut(s) 143, 256, 295, 388
LmnI GCTCC 5 cut(s) 190, 347, 516, 575, 932
Lsp1109I GCAGC 2 cut(s) 394, 397
LweI GCATC 3 cut(s) 394, 829, 856
MaeI CTAG 4 cut(s) 293, 416, 1043, 1079
MaeIII GTNAC 5 cut(s) 200, 607, 737, 845, 1026
MalI GATC 4 cut(s) 145, 258, 297, 390
MboI GATC 4 cut(s) 143, 256, 295, 388
MboII GAAGA 4 cut(s) 59, 388, 593, 889
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 4 cut(s) 33, 111, 513, 823
MluCI AATT 9 cut(s) 17, 51, 87, 376, 439, 492, 655, 698, 1011
MlyI GAGTC 4 cut(s) 145, 755, 820, 987
MmeI TCCRAC 2 cut(s) 79, 618
MnlI CCTC 9 cut(s) 56, 74, 304, 526, 661, 809, 871, 946, 1107
MroXI GAANNNNTTC 1 cut(s) 51
MseI TTAA 3 cut(s) 86, 116, 885
MslI CAYNNNNRTG 1 cut(s) 1068
MspI CCGG 1 cut(s) 813
MspR9I CCNGG 4 cut(s) 427, 713, 780, 1038
MvaI CCWGG 4 cut(s) 427, 713, 780, 1038
MvnI CGCG 1 cut(s) 915
MwoI GCNNNNNNNGC 3 cut(s) 818, 866, 912
NcoI CCATGG 1 cut(s) 984
NdeII GATC 4 cut(s) 143, 256, 295, 388
NlaIII CATG 7 cut(s) 307, 473, 598, 725, 944, 988, 1061
NlaIV GGNNCC 6 cut(s) 31, 343, 365, 431, 467, 934
NmuCI GTSAC 2 cut(s) 607, 845
NruI TCGCGA 1 cut(s) 915
PagI TCATGA 1 cut(s) 721
PdmI GAANNNNTTC 1 cut(s) 51
PfeI GAWTC 1 cut(s) 789
PflMI CCANNNNNTGG 2 cut(s) 39, 77
PfoI TCCNGGA 1 cut(s) 1036
PkrI GCNGC 3 cut(s) 359, 409, 412
PleI GAGTC 4 cut(s) 145, 754, 820, 986
PpsI GAGTC 4 cut(s) 145, 754, 820, 986
PpuMI RGGWCCY 1 cut(s) 921
PsiI TTATAA 1 cut(s) 629
Psp124BI GAGCTC 1 cut(s) 513
Psp5II RGGWCCY 1 cut(s) 921
Psp6I CCWGG 4 cut(s) 425, 711, 778, 1036
PspFI CCCAGC 1 cut(s) 232
PspGI CCWGG 4 cut(s) 425, 711, 778, 1036
PspN4I GGNNCC 6 cut(s) 31, 343, 365, 431, 467, 934
PspOMI GGGCCC 1 cut(s) 29
PspPI GGNCC 7 cut(s) 29, 30, 364, 430, 503, 921, 1034
PspPPI RGGWCCY 1 cut(s) 921
PsuI RGATCY 1 cut(s) 295
RruI TCGCGA 1 cut(s) 915
RsaI GTAC 2 cut(s) 179, 1076
RsaNI GTAC 2 cut(s) 178, 1075
RseI CAYNNNNRTG 1 cut(s) 1068
SacI GAGCTC 1 cut(s) 513
SaqAI TTAA 3 cut(s) 86, 116, 885
SatI GCNGC 3 cut(s) 358, 408, 411
Sau3AI GATC 4 cut(s) 143, 256, 295, 388
Sau96I GGNCC 7 cut(s) 29, 30, 364, 430, 503, 921, 1034
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 4 cut(s) 145, 755, 820, 987
ScrFI CCNGG 4 cut(s) 427, 713, 780, 1038
SduI GDGCHC 4 cut(s) 33, 111, 513, 823
SfaNI GCATC 3 cut(s) 394, 829, 856
SinI GGWCC 5 cut(s) 364, 430, 503, 921, 1034
SmiMI CAYNNNNRTG 1 cut(s) 1068
Sse9I AATT 9 cut(s) 17, 51, 87, 376, 439, 492, 655, 698, 1011
SsiI CCGC 3 cut(s) 355, 358, 476
SspMI CTAG 4 cut(s) 293, 416, 1043, 1079
SstI GAGCTC 1 cut(s) 513
StyD4I CCNGG 4 cut(s) 425, 711, 778, 1036
StyI CCWWGG 1 cut(s) 984
TaaI ACNGT 3 cut(s) 383, 613, 851
TaqI TCGA 1 cut(s) 228
TasI AATT 9 cut(s) 17, 51, 87, 376, 439, 492, 655, 698, 1011
TatI WGTACW 1 cut(s) 177
TauI GCSGC 1 cut(s) 360
TfiI GAWTC 1 cut(s) 789
Tru1I TTAA 3 cut(s) 86, 116, 885
Tru9I TTAA 3 cut(s) 86, 116, 885
TscAI CASTG 5 cut(s) 211, 531, 616, 828, 979
TseFI GTSAC 2 cut(s) 607, 845
TseI GCWGC 2 cut(s) 407, 410
Tsp45I GTSAC 2 cut(s) 607, 845
TspDTI ATGAA 3 cut(s) 60, 738, 781
TspGWI ACGGA 1 cut(s) 202
TspRI CASTG 5 cut(s) 211, 531, 616, 828, 979
Van91I CCANNNNNTGG 2 cut(s) 39, 77
VneI GTGCAC 1 cut(s) 819
VpaK11BI GGWCC 5 cut(s) 364, 430, 503, 921, 1034
XapI RAATTY 2 cut(s) 51, 655
XmiI GTMKAC 1 cut(s) 682
XmnI GAANNNNTTC 1 cut(s) 51
XspI CTAG 4 cut(s) 293, 416, 1043, 1079
ZrmI AGTACT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.