Rh7DG108400

FIST_C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0010565
Physical Location & Seq
Forward (+)
9314564 .. 9331788
17225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG108400.1

Sequence Viewer

Length: 375 bp
ATGTCTGTGAACCCAGACCCCTGTGAGGTTGTGGATGAGGTTTTCTCTCAGCCGATTCGTCCCGACTTTGCAATTGTTTACATTGGCATGGAGTTTAGCTTGCCATTCACAAGCCTCTTTGTCTTCAAAAAGGTACCGCGTGGAGTTCCAATCATCATCAATCAAGCTGTTGGGCTAATCGGCGTCGATGCTGGAGCAGATGAAATCTTAGAGGTGAAGTGGGAATTGGAAAATGGGCCCAACTATTGGGATGAAGAAATTCTAATGGGAGGTAATCCAATAAATGGAGGGATTCTCTCTGTTGGATTTGTGCCCGATTTAAAAGTGGATGTCATCCCACTTCCAAGATCAAAGACTCAACCCAATATGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FIST_C

Protein Analysis

124

Amino Acids

13.61

Weight (kDa)

4.23

Isoelectric Point (pI)

25.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 133
AccB1I GGYRCC 1 cut(s) 133
AccB7I CCANNNNNTGG 2 cut(s) 246, 284
AccII CGCG 1 cut(s) 139
AciI CCGC 1 cut(s) 137
AcsI RAATTY 1 cut(s) 258
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 1 cut(s) 135
AfiI CCNNNNNNNGG 3 cut(s) 25, 246, 284
AgsI TTSAA 1 cut(s) 127
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AluBI AGCT 2 cut(s) 99, 167
AluI AGCT 2 cut(s) 99, 167
AoxI GGCC 1 cut(s) 236
ApaI GGGCCC 1 cut(s) 240
ApoI RAATTY 1 cut(s) 258
Asp700I GAANNNNTTC 1 cut(s) 258
Asp718I GGTACC 1 cut(s) 133
AspS9I GGNCC 2 cut(s) 236, 237
AsuHPI GGTGA 1 cut(s) 226
BaeGI GKGCMC 2 cut(s) 240, 315
BanI GGYRCC 1 cut(s) 133
BanII GRGCYC 1 cut(s) 240
BbsI GAAGAC 1 cut(s) 115
BmgT120I GGNCC 2 cut(s) 236, 237
BmiI GGNNCC 2 cut(s) 135, 238
BmsI GCATC 1 cut(s) 178
BpiI GAAGAC 1 cut(s) 115
BplI GAGNNNNNCTC 4 cut(s) 29, 61, 279, 311
BpmI CTGGAG 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 183
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 246, 284
BseGI GGATG 4 cut(s) 40, 256, 333, 334
BseLI CCNNNNNNNGG 3 cut(s) 25, 246, 284
BseMII CTCAG 1 cut(s) 62
BseSI GKGCMC 2 cut(s) 240, 315
Bsh1236I CGCG 1 cut(s) 139
BshFI GGCC 1 cut(s) 238
BshNI GGYRCC 1 cut(s) 133
BslFI GGGAC 1 cut(s) 45
BslI CCNNNNNNNGG 3 cut(s) 25, 246, 284
BsmFI GGGAC 1 cut(s) 45
BsnI GGCC 1 cut(s) 238
Bsp120I GGGCCC 1 cut(s) 236
Bsp1286I GDGCHC 2 cut(s) 240, 315
Bsp143I GATC 1 cut(s) 347
BspACI CCGC 1 cut(s) 137
BspANI GGCC 1 cut(s) 238
BspCNI CTCAG 1 cut(s) 61
BspFNI CGCG 1 cut(s) 139
BspLI GGNNCC 2 cut(s) 135, 238
BspT107I GGYRCC 1 cut(s) 133
BssMI GATC 1 cut(s) 347
BssNI GRCGYC 1 cut(s) 183
BstACI GRCGYC 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 101
BstDEI CTNAG 2 cut(s) 48, 208
BstF5I GGATG 4 cut(s) 40, 256, 333, 334
BstFNI CGCG 1 cut(s) 139
BstKTI GATC 1 cut(s) 350
BstMBI GATC 1 cut(s) 347
BstSLI GKGCMC 2 cut(s) 240, 315
BstUI CGCG 1 cut(s) 139
BstV2I GAAGAC 1 cut(s) 115
BsuRI GGCC 1 cut(s) 238
BtsCI GGATG 4 cut(s) 40, 256, 333, 334
Cac8I GCNNGC 1 cut(s) 101
Cfr13I GGNCC 2 cut(s) 236, 237
CseI GACGC 1 cut(s) 172
Csp6I GTAC 1 cut(s) 134
CviAII CATG 1 cut(s) 88
CviJI RGCY 6 cut(s) 52, 99, 114, 167, 175, 238
CviKI_1 RGCY 6 cut(s) 52, 99, 114, 167, 175, 238
CviQI GTAC 1 cut(s) 134
DdeI CTNAG 2 cut(s) 48, 208
DpnI GATC 1 cut(s) 349
DpnII GATC 1 cut(s) 347
DraI TTTAAA 1 cut(s) 321
Eco24I GRGCYC 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 240
FaeI CATG 1 cut(s) 91
FaiI YATR 3 cut(s) 89, 368, 373
FaqI GGGAC 1 cut(s) 45
FatI CATG 1 cut(s) 87
FokI GGATG 4 cut(s) 47, 263, 320, 341
FriOI GRGCYC 1 cut(s) 240
GsuI CTGGAG 1 cut(s) 213
HaeIII GGCC 1 cut(s) 238
HgaI GACGC 1 cut(s) 172
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 1 cut(s) 91
HinfI GANTC 3 cut(s) 55, 292, 355
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 2 cut(s) 10, 79
Hpy188III TCNNGA 1 cut(s) 62
Hpy8I GTNNAC 2 cut(s) 10, 79
Hpy99I CGWCG 1 cut(s) 188
HpyCH4V TGCA 1 cut(s) 71
HpyF3I CTNAG 2 cut(s) 48, 208
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 1 cut(s) 91
KpnI GGTACC 1 cut(s) 137
Kzo9I GATC 1 cut(s) 347
LmnI GCTCC 1 cut(s) 194
LpnPI CCDG 3 cut(s) 27, 34, 177
LweI GCATC 1 cut(s) 178
MalI GATC 1 cut(s) 349
MboI GATC 1 cut(s) 347
MboII GAAGA 2 cut(s) 115, 266
MfeI CAATTG 1 cut(s) 72
MhlI GDGCHC 2 cut(s) 240, 315
MluCI AATT 3 cut(s) 72, 224, 258
MlyI GAGTC 1 cut(s) 349
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 6 cut(s) 19, 31, 125, 205, 263, 281
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 1 cut(s) 320
MslI CAYNNNNRTG 1 cut(s) 86
MunI CAATTG 1 cut(s) 72
MvnI CGCG 1 cut(s) 139
NdeII GATC 1 cut(s) 347
NlaIII CATG 1 cut(s) 91
NlaIV GGNNCC 2 cut(s) 135, 238
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 2 cut(s) 55, 292
PflMI CCANNNNNTGG 2 cut(s) 246, 284
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
PspN4I GGNNCC 2 cut(s) 135, 238
PspOMI GGGCCC 1 cut(s) 236
PspPI GGNCC 2 cut(s) 236, 237
RsaI GTAC 1 cut(s) 135
RsaNI GTAC 1 cut(s) 134
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 1 cut(s) 320
Sau3AI GATC 1 cut(s) 347
Sau96I GGNCC 2 cut(s) 236, 237
SchI GAGTC 1 cut(s) 349
SduI GDGCHC 2 cut(s) 240, 315
SetI ASST 7 cut(s) 30, 42, 101, 135, 169, 216, 274
SfaNI GCATC 1 cut(s) 178
SmiMI CAYNNNNRTG 1 cut(s) 86
Sse9I AATT 3 cut(s) 72, 224, 258
SsiI CCGC 1 cut(s) 137
TaqI TCGA 1 cut(s) 186
TasI AATT 3 cut(s) 72, 224, 258
TfiI GAWTC 2 cut(s) 55, 292
Tru1I TTAA 1 cut(s) 320
Tru9I TTAA 1 cut(s) 320
TspDTI ATGAA 2 cut(s) 216, 267
Van91I CCANNNNNTGG 2 cut(s) 246, 284
XapI RAATTY 1 cut(s) 258
XmnI GAANNNNTTC 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.