Rh7BG108200

FIST_C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0010565
Physical Location & Seq
Forward (+)
8174489 .. 8177824
3336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG108200.1

Sequence Viewer

Length: 1113 bp
ATGCAGGTGAAGTGGGAATTGGAAAATGGGCCCAACTATTGGGATGAAGAAATTCTAATGGGAGGTAATCCAATAAATGGAGGGTTAATTCTCTCTGTTGGATTTGTGCCCGATTTAAAAGTGGATGTCATCCCACTTCCAAGATCAAAGACTCAACCCAATATGCCATTGATGGAGTACTTTATGCTGGAGCTTAGGAGTTACACTGCTTCCGTTTCAGGTTCTCTCGACCCAGCTTGTATCACAATGTTCGGAGATCATCGTGTTGATATAAGACCCATTGTTGAGAAACTAGATCTTGTCATGCCTGAGGAGACTGCCGTTGTAGGAGACGCAAAAGGCTCCTTTCTGTTCCGCCGCAGGGACCAAACCCAAAATTACAGTGCTGATCTGTTCTTCTTTGATGCTGCTGCTCTAGTATTTGCCAGGGACCAAAACAAGTCTGCTGAGTTAGGGGAAACTCGGTTCCATGTGGCGGTGGCCGACGGCATAATTCCATTTGGTCCTGAGCTCAGGAGCGTTTCAGTGTTTGAGGATTTCTTTGCTCAATCTTGGATTTCGGGCGTAGTGGAAGGAGCAGAAGAAGGGTATCCCATGCTTGATTGCGTCACTGTTTTACAACAGCTTATAAATCAGTTGGACTTGGGAAACCCAAATTTATACATTGAGGTCAGGAAAGGTAGACAATACTCCACACAATTCAGAACAACCCTGGCTTGTCATGAACTTTTGGGGGGTAACTACGAGTCCATAATCGTCAAGGGTTGTGGCATACAACCTGGGGATAGATTCATATTCCTCCACTCAGACACCGGAAGTGCACTGACTCGTGTTGACGATGCTCGTCACAGTTTGGAAGCATTGGATGCTAATCAAGAGGTGTTTGGAGGCTTGATCTTCTCTAGCTACCACCGCGACAGGTCCTTTTTCGCAGCCTCTCATGTGGATATAACTCCCTTTACTGACAAGCTCAGTGGAGTTCCCATGGCTGGTATCTCCTGCACAAGAGAAATTGGGCGTGGGGCGTTACTTGGTCCAGGACTAGGGTATTGCCTCCATGCACATAGCTGTGTGTACCTAGCTATGTCTTATGTTCCTCCCATGTCCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FIST_C

Protein Analysis

370

Amino Acids

40.95

Weight (kDa)

4.9

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 629
AccB7I CCANNNNNTGG 2 cut(s) 39, 77
AccI GTMKAC 1 cut(s) 682
AccII CGCG 1 cut(s) 915
AciI CCGC 4 cut(s) 355, 358, 476, 913
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 2 cut(s) 51, 655
AfaI GTAC 2 cut(s) 179, 1076
AfiI CCNNNNNNNGG 6 cut(s) 39, 77, 361, 475, 989, 1043
AjnI CCWGG 4 cut(s) 425, 711, 778, 1036
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AloI GAACNNNNNNTCC 2 cut(s) 449, 481
AluBI AGCT 8 cut(s) 193, 236, 511, 625, 906, 970, 1068, 1082
AluI AGCT 8 cut(s) 193, 236, 511, 625, 906, 970, 1068, 1082
Alw21I GWGCWC 2 cut(s) 513, 823
Alw26I GTCTC 2 cut(s) 308, 324
Alw44I GTGCAC 1 cut(s) 819
AoxI GGCC 2 cut(s) 29, 480
ApaI GGGCCC 1 cut(s) 33
ApaLI GTGCAC 1 cut(s) 819
ApeKI GCWGC 3 cut(s) 407, 410, 932
ApoI RAATTY 2 cut(s) 51, 655
Asp700I GAANNNNTTC 1 cut(s) 51
AspS9I GGNCC 7 cut(s) 29, 30, 364, 430, 503, 921, 1034
AsuHPI GGTGA 1 cut(s) 19
AvaII GGWCC 5 cut(s) 364, 430, 503, 921, 1034
AxyI CCTNAGG 1 cut(s) 309
BaeGI GKGCMC 3 cut(s) 33, 111, 823
BanII GRGCYC 2 cut(s) 33, 513
BauI CACGAG 1 cut(s) 828
Bbv12I GWGCWC 2 cut(s) 513, 823
BbvI GCAGC 3 cut(s) 394, 397, 944
BccI CCATC 1 cut(s) 166
BceAI ACGGC 2 cut(s) 305, 502
BciT130I CCWGG 4 cut(s) 427, 713, 780, 1038
BciVI GTATCC 1 cut(s) 600
BcoDI GTCTC 2 cut(s) 308, 324
BfaI CTAG 5 cut(s) 293, 416, 903, 1043, 1079
BfuI GTATCC 1 cut(s) 600
BglII AGATCT 1 cut(s) 295
BisI GCNGC 4 cut(s) 358, 408, 411, 933
BlsI GCNGC 4 cut(s) 359, 409, 412, 934
BmcAI AGTACT 1 cut(s) 179
Bme1390I CCNGG 4 cut(s) 427, 713, 780, 1038
Bme18I GGWCC 5 cut(s) 364, 430, 503, 921, 1034
BmgT120I GGNCC 7 cut(s) 29, 30, 364, 430, 503, 921, 1034
BmiI GGNNCC 5 cut(s) 31, 343, 365, 431, 467
BmrFI CCNGG 4 cut(s) 427, 713, 780, 1038
BmsI GCATC 3 cut(s) 394, 829, 856
BpmI CTGGAG 1 cut(s) 209
Bpu10I CCTNAGC 3 cut(s) 194, 507, 512
BsaBI GATNNNNATC 1 cut(s) 870
BsaJI CCNNGG 4 cut(s) 426, 711, 779, 984
BsaWI WCCGGW 1 cut(s) 812
Bsc4I CCNNNNNNNGG 6 cut(s) 39, 77, 361, 475, 989, 1043
Bse21I CCTNAGG 1 cut(s) 309
Bse8I GATNNNNATC 1 cut(s) 870
BseBI CCWGG 4 cut(s) 427, 713, 780, 1038
BseDI CCNNGG 4 cut(s) 426, 711, 779, 984
BseGI GGATG 4 cut(s) 49, 129, 130, 871
BseJI GATNNNNATC 1 cut(s) 870
BseLI CCNNNNNNNGG 6 cut(s) 39, 77, 361, 475, 989, 1043
BseMII CTCAG 6 cut(s) 300, 438, 498, 526, 819, 985
BseRI GAGGAG 1 cut(s) 326
BseSI GKGCMC 3 cut(s) 33, 111, 823
BseXI GCAGC 3 cut(s) 394, 397, 944
BseYI CCCAGC 1 cut(s) 232
BsgI GTGCAG 1 cut(s) 985
Bsh1236I CGCG 1 cut(s) 915
BshFI GGCC 2 cut(s) 31, 482
BsiHKAI GWGCWC 2 cut(s) 513, 823
BsiSI CCGG 1 cut(s) 813
BslFI GGGAC 2 cut(s) 377, 443
BslI CCNNNNNNNGG 6 cut(s) 39, 77, 361, 475, 989, 1043
BsmAI GTCTC 2 cut(s) 308, 324
BsmBI CGTCTC 1 cut(s) 324
BsmFI GGGAC 2 cut(s) 377, 443
BsnI GGCC 2 cut(s) 31, 482
Bsp120I GGGCCC 1 cut(s) 29
Bsp1286I GDGCHC 4 cut(s) 33, 111, 513, 823
Bsp143I GATC 5 cut(s) 143, 256, 295, 388, 894
Bsp19I CCATGG 1 cut(s) 984
BspACI CCGC 4 cut(s) 355, 358, 476, 913
BspANI GGCC 2 cut(s) 31, 482
BspCNI CTCAG 6 cut(s) 301, 439, 499, 525, 818, 984
BspFNI CGCG 1 cut(s) 915
BspHI TCATGA 1 cut(s) 721
BspLI GGNNCC 5 cut(s) 31, 343, 365, 431, 467
BssECI CCNNGG 4 cut(s) 426, 711, 779, 984
BssMI GATC 5 cut(s) 143, 256, 295, 388, 894
BssSI CACGAG 1 cut(s) 828
BssT1I CCWWGG 1 cut(s) 984
Bst2BI CACGAG 1 cut(s) 828
Bst2UI CCWGG 4 cut(s) 427, 713, 780, 1038
Bst4CI ACNGT 3 cut(s) 383, 613, 851
BstAPI GCANNNNNTGC 1 cut(s) 866
BstDEI CTNAG 7 cut(s) 194, 309, 447, 507, 512, 805, 971
BstDSI CCRYGG 1 cut(s) 984
BstF5I GGATG 4 cut(s) 49, 129, 130, 871
BstFNI CGCG 1 cut(s) 915
BstKTI GATC 5 cut(s) 146, 259, 298, 391, 897
BstMAI GTCTC 2 cut(s) 308, 324
BstMBI GATC 5 cut(s) 143, 256, 295, 388, 894
BstMWI GCNNNNNNNGC 2 cut(s) 866, 912
BstNI CCWGG 4 cut(s) 427, 713, 780, 1038
BstSCI CCNGG 4 cut(s) 425, 711, 778, 1036
BstSLI GKGCMC 3 cut(s) 33, 111, 823
BstUI CGCG 1 cut(s) 915
BstV1I GCAGC 3 cut(s) 394, 397, 944
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
Bsu36I CCTNAGG 1 cut(s) 309
BsuI GTATCC 1 cut(s) 600
BsuRI GGCC 2 cut(s) 31, 482
BtgI CCRYGG 1 cut(s) 984
BtsCI GGATG 4 cut(s) 49, 129, 130, 871
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 6 cut(s) 204, 388, 531, 609, 821, 979
CciI TCATGA 1 cut(s) 721
Cfr13I GGNCC 7 cut(s) 29, 30, 364, 430, 503, 921, 1034
CseI GACGC 2 cut(s) 341, 595
Csp6I GTAC 2 cut(s) 178, 1075
CspCI CAANNNNNGTGG 4 cut(s) 748, 783, 955, 990
CviAII CATG 8 cut(s) 304, 470, 595, 722, 941, 985, 1058, 1102
CviQI GTAC 2 cut(s) 178, 1075
DdeI CTNAG 7 cut(s) 194, 309, 447, 507, 512, 805, 971
DpnI GATC 5 cut(s) 145, 258, 297, 390, 896
DpnII GATC 5 cut(s) 143, 256, 295, 388, 894
DraI TTTAAA 1 cut(s) 117
EaeI YGGCCR 1 cut(s) 480
EciI GGCGGA 1 cut(s) 344
Ecl136II GAGCTC 1 cut(s) 511
Eco130I CCWWGG 1 cut(s) 984
Eco24I GRGCYC 2 cut(s) 33, 513
Eco47I GGWCC 5 cut(s) 364, 430, 503, 921, 1034
Eco53kI GAGCTC 1 cut(s) 511
Eco81I CCTNAGG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 511
EcoO109I RGGNCCY 1 cut(s) 921
EcoRII CCWGG 4 cut(s) 425, 711, 778, 1036
EcoT14I CCWWGG 1 cut(s) 984
EcoT38I GRGCYC 2 cut(s) 33, 513
ErhI CCWWGG 1 cut(s) 984
Esp3I CGTCTC 1 cut(s) 324
FaeI CATG 8 cut(s) 307, 473, 598, 725, 944, 988, 1061, 1105
FaqI GGGAC 2 cut(s) 377, 443
FatI CATG 8 cut(s) 303, 469, 594, 721, 940, 984, 1057, 1101
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 4 cut(s) 358, 408, 411, 933
FokI GGATG 4 cut(s) 56, 116, 137, 878
FriOI GRGCYC 2 cut(s) 33, 513
Fsp4HI GCNGC 4 cut(s) 358, 408, 411, 933
FspBI CTAG 5 cut(s) 293, 416, 903, 1043, 1079
GluI GCNGC 4 cut(s) 358, 408, 411, 933
GsaI CCCAGC 1 cut(s) 236
GsuI CTGGAG 1 cut(s) 209
HaeIII GGCC 2 cut(s) 31, 482
HapII CCGG 1 cut(s) 813
HgaI GACGC 2 cut(s) 341, 595
Hin1II CATG 8 cut(s) 307, 473, 598, 725, 944, 988, 1061, 1105
HincII GTYRAC 1 cut(s) 835
HindII GTYRAC 1 cut(s) 835
HinfI GANTC 4 cut(s) 151, 746, 789, 826
HpaII CCGG 1 cut(s) 813
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 4 cut(s) 683, 821, 835, 1075
Hpy188I TCNGA 3 cut(s) 254, 704, 808
Hpy188III TCNNGA 6 cut(s) 227, 506, 514, 673, 722, 875
Hpy8I GTNNAC 4 cut(s) 683, 821, 835, 1075
Hpy99I CGWCG 1 cut(s) 488
HpyAV CCTTC 2 cut(s) 566, 578
HpyCH4III ACNGT 3 cut(s) 383, 613, 851
HpyCH4V TGCA 4 cut(s) 4, 821, 1002, 1061
HpyF10VI GCNNNNNNNGC 2 cut(s) 866, 912
HpyF3I CTNAG 7 cut(s) 194, 309, 447, 507, 512, 805, 971
Hsp92II CATG 8 cut(s) 307, 473, 598, 725, 944, 988, 1061, 1105
Kzo9I GATC 5 cut(s) 143, 256, 295, 388, 894
LmnI GCTCC 4 cut(s) 190, 347, 516, 575
Lsp1109I GCAGC 3 cut(s) 394, 397, 944
LweI GCATC 3 cut(s) 394, 829, 856
MaeI CTAG 5 cut(s) 293, 416, 903, 1043, 1079
MaeIII GTNAC 5 cut(s) 200, 607, 737, 845, 1026
MalI GATC 5 cut(s) 145, 258, 297, 390, 896
MboI GATC 5 cut(s) 143, 256, 295, 388, 894
MboII GAAGA 4 cut(s) 59, 388, 593, 889
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 4 cut(s) 33, 111, 513, 823
MluCI AATT 8 cut(s) 17, 51, 87, 376, 492, 655, 698, 1011
MlyI GAGTC 3 cut(s) 145, 755, 820
MmeI TCCRAC 2 cut(s) 79, 618
MroXI GAANNNNTTC 1 cut(s) 51
MseI TTAA 2 cut(s) 86, 116
MslI CAYNNNNRTG 1 cut(s) 1068
MspI CCGG 1 cut(s) 813
MspR9I CCNGG 4 cut(s) 427, 713, 780, 1038
MvaI CCWGG 4 cut(s) 427, 713, 780, 1038
MvnI CGCG 1 cut(s) 915
MwoI GCNNNNNNNGC 2 cut(s) 866, 912
NcoI CCATGG 1 cut(s) 984
NdeII GATC 5 cut(s) 143, 256, 295, 388, 894
NlaIII CATG 8 cut(s) 307, 473, 598, 725, 944, 988, 1061, 1105
NlaIV GGNNCC 5 cut(s) 31, 343, 365, 431, 467
NmuCI GTSAC 2 cut(s) 607, 845
PagI TCATGA 1 cut(s) 721
PdmI GAANNNNTTC 1 cut(s) 51
PfeI GAWTC 1 cut(s) 789
PflMI CCANNNNNTGG 2 cut(s) 39, 77
PfoI TCCNGGA 1 cut(s) 1036
PkrI GCNGC 4 cut(s) 359, 409, 412, 934
PleI GAGTC 3 cut(s) 145, 754, 820
PpsI GAGTC 3 cut(s) 145, 754, 820
PpuMI RGGWCCY 1 cut(s) 921
PsiI TTATAA 1 cut(s) 629
Psp124BI GAGCTC 1 cut(s) 513
Psp5II RGGWCCY 1 cut(s) 921
Psp6I CCWGG 4 cut(s) 425, 711, 778, 1036
PspFI CCCAGC 1 cut(s) 232
PspGI CCWGG 4 cut(s) 425, 711, 778, 1036
PspN4I GGNNCC 5 cut(s) 31, 343, 365, 431, 467
PspOMI GGGCCC 1 cut(s) 29
PspPI GGNCC 7 cut(s) 29, 30, 364, 430, 503, 921, 1034
PspPPI RGGWCCY 1 cut(s) 921
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 2 cut(s) 179, 1076
RsaNI GTAC 2 cut(s) 178, 1075
RseI CAYNNNNRTG 1 cut(s) 1068
SacI GAGCTC 1 cut(s) 513
SaqAI TTAA 2 cut(s) 86, 116
SatI GCNGC 4 cut(s) 358, 408, 411, 933
Sau3AI GATC 5 cut(s) 143, 256, 295, 388, 894
Sau96I GGNCC 7 cut(s) 29, 30, 364, 430, 503, 921, 1034
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 3 cut(s) 145, 755, 820
ScrFI CCNGG 4 cut(s) 427, 713, 780, 1038
SduI GDGCHC 4 cut(s) 33, 111, 513, 823
SfaNI GCATC 3 cut(s) 394, 829, 856
SinI GGWCC 5 cut(s) 364, 430, 503, 921, 1034
SmiMI CAYNNNNRTG 1 cut(s) 1068
Sse9I AATT 8 cut(s) 17, 51, 87, 376, 492, 655, 698, 1011
SsiI CCGC 4 cut(s) 355, 358, 476, 913
SspMI CTAG 5 cut(s) 293, 416, 903, 1043, 1079
SstI GAGCTC 1 cut(s) 513
StyD4I CCNGG 4 cut(s) 425, 711, 778, 1036
StyI CCWWGG 1 cut(s) 984
TaaI ACNGT 3 cut(s) 383, 613, 851
TaqI TCGA 1 cut(s) 228
TasI AATT 8 cut(s) 17, 51, 87, 376, 492, 655, 698, 1011
TatI WGTACW 1 cut(s) 177
TauI GCSGC 1 cut(s) 360
TfiI GAWTC 1 cut(s) 789
Tru1I TTAA 2 cut(s) 86, 116
Tru9I TTAA 2 cut(s) 86, 116
TscAI CASTG 6 cut(s) 211, 388, 531, 616, 828, 979
TseFI GTSAC 2 cut(s) 607, 845
TseI GCWGC 3 cut(s) 407, 410, 932
Tsp45I GTSAC 2 cut(s) 607, 845
TspDTI ATGAA 3 cut(s) 60, 738, 781
TspGWI ACGGA 1 cut(s) 202
TspRI CASTG 6 cut(s) 211, 388, 531, 616, 828, 979
Van91I CCANNNNNTGG 2 cut(s) 39, 77
VneI GTGCAC 1 cut(s) 819
VpaK11BI GGWCC 5 cut(s) 364, 430, 503, 921, 1034
XapI RAATTY 2 cut(s) 51, 655
XmiI GTMKAC 1 cut(s) 682
XmnI GAANNNNTTC 1 cut(s) 51
XspI CTAG 5 cut(s) 293, 416, 903, 1043, 1079
ZrmI AGTACT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.