Rw7G009130

FIST_C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0010565
Physical Location & Seq
Forward (+)
8612051 .. 8613036
986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G009130.1

Sequence Viewer

Length: 519 bp
ATGCTTGATTGCGTCACTGTTTTACAACAACTTATAAATCAGTTGGACTTGGGGGTTCCAAATTTATACATTGAGGTCAGGAAAGGTAGACAATACTCCACACAATTCAGAACAACCCCGGCTTGTCATGAACTTTTGGGGGGTAACTACGAGTCCATAATCGTCAAGGGTTGTGGCATACAACCTGGGGATAGATTCATATTCCTCCACTCAGACGCCGGAAGTGCACTGATTCGTGTTGACGATGCTCGTCACAGTTTGGAAGCATTGGATGCTAATCAAGAGGTGTTTGGAGGCTTGATCTTCTCCAGCTACCATCGCGACAGATCCTTTTTCGGAGCCTCTCATGTGGATATGAATCCGTTTACTGACAAGCTCAGTGGAGTCCCCATGGCTGGTATCTCCTGCACAAGAGAAATTGGGCGTGGGGCGTTACTTGGTCCAGGACTAGGGTATTGCGTCCATGCACATACCTGTGTGTACCTAGCTATGTCTTATGTTCCTCCTATGTCCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FIST_C

Protein Analysis

172

Amino Acids

18.86

Weight (kDa)

6.13

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 35
AccI GTMKAC 1 cut(s) 88
AccII CGCG 1 cut(s) 321
AclWI GGATC 1 cut(s) 321
AcsI RAATTY 1 cut(s) 61
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 1 cut(s) 482
AfiI CCNNNNNNNGG 2 cut(s) 395, 449
AjnI CCWGG 2 cut(s) 184, 442
AluBI AGCT 3 cut(s) 312, 376, 488
AluI AGCT 3 cut(s) 312, 376, 488
Alw21I GWGCWC 1 cut(s) 229
Alw44I GTGCAC 1 cut(s) 225
AlwI GGATC 1 cut(s) 321
ApaLI GTGCAC 1 cut(s) 225
ApoI RAATTY 1 cut(s) 61
AspS9I GGNCC 1 cut(s) 440
AsuC2I CCSGG 1 cut(s) 119
AvaII GGWCC 1 cut(s) 440
BaeGI GKGCMC 1 cut(s) 229
Bbv12I GWGCWC 1 cut(s) 229
BccI CCATC 1 cut(s) 324
BciT130I CCWGG 2 cut(s) 186, 444
BcnI CCSGG 1 cut(s) 119
BfaI CTAG 2 cut(s) 449, 485
Bme1390I CCNGG 3 cut(s) 119, 186, 444
Bme18I GGWCC 1 cut(s) 440
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 2 cut(s) 57, 340
BmrFI CCNGG 3 cut(s) 119, 186, 444
BmsI GCATC 2 cut(s) 235, 262
BpmI CTGGAG 1 cut(s) 292
BpuMI CCSGG 1 cut(s) 119
BsaBI GATNNNNATC 2 cut(s) 276, 357
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 3 cut(s) 117, 185, 390
Bsc4I CCNNNNNNNGG 2 cut(s) 395, 449
Bse8I GATNNNNATC 2 cut(s) 276, 357
BseBI CCWGG 2 cut(s) 186, 444
BseDI CCNNGG 3 cut(s) 117, 185, 390
BseGI GGATG 1 cut(s) 277
BseJI GATNNNNATC 2 cut(s) 276, 357
BseLI CCNNNNNNNGG 2 cut(s) 395, 449
BseMII CTCAG 2 cut(s) 225, 391
BseSI GKGCMC 1 cut(s) 229
BsgI GTGCAG 1 cut(s) 391
Bsh1236I CGCG 1 cut(s) 321
BsiHKAI GWGCWC 1 cut(s) 229
BsiSI CCGG 2 cut(s) 119, 219
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 2 cut(s) 395, 449
BsmFI GGGAC 1 cut(s) 371
Bsp1286I GDGCHC 1 cut(s) 229
Bsp143I GATC 2 cut(s) 300, 326
Bsp19I CCATGG 1 cut(s) 390
Bsp68I TCGCGA 1 cut(s) 321
BspCNI CTCAG 2 cut(s) 224, 390
BspFNI CGCG 1 cut(s) 321
BspHI TCATGA 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 57, 340
BspPI GGATC 1 cut(s) 321
BssECI CCNNGG 3 cut(s) 117, 185, 390
BssMI GATC 2 cut(s) 300, 326
BssNI GRCGYC 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 390
Bst2UI CCWGG 2 cut(s) 186, 444
Bst4CI ACNGT 2 cut(s) 19, 257
BstACI GRCGYC 1 cut(s) 216
BstAPI GCANNNNNTGC 1 cut(s) 272
BstDEI CTNAG 2 cut(s) 211, 377
BstDSI CCRYGG 1 cut(s) 390
BstF5I GGATG 1 cut(s) 277
BstFNI CGCG 1 cut(s) 321
BstKTI GATC 2 cut(s) 303, 329
BstMBI GATC 2 cut(s) 300, 326
BstMWI GCNNNNNNNGC 3 cut(s) 224, 272, 318
BstNI CCWGG 2 cut(s) 186, 444
BstSCI CCNGG 3 cut(s) 117, 184, 442
BstSLI GKGCMC 1 cut(s) 229
BstUI CGCG 1 cut(s) 321
BstX2I RGATCY 1 cut(s) 326
BstYI RGATCY 1 cut(s) 326
BtgI CCRYGG 1 cut(s) 390
BtgZI GCGATG 1 cut(s) 302
BtsCI GGATG 1 cut(s) 277
BtsIMutI CAGTG 3 cut(s) 15, 227, 385
BtuMI TCGCGA 1 cut(s) 321
CciI TCATGA 1 cut(s) 127
Cfr13I GGNCC 1 cut(s) 440
CseI GACGC 2 cut(s) 224, 448
Csp6I GTAC 1 cut(s) 481
CspCI CAANNNNNGTGG 4 cut(s) 154, 189, 361, 396
CviAII CATG 4 cut(s) 128, 347, 391, 464
CviJI RGCY 7 cut(s) 122, 297, 312, 341, 376, 395, 488
CviKI_1 RGCY 7 cut(s) 122, 297, 312, 341, 376, 395, 488
CviQI GTAC 1 cut(s) 481
DdeI CTNAG 2 cut(s) 211, 377
DpnI GATC 2 cut(s) 302, 328
DpnII GATC 2 cut(s) 300, 326
Eco130I CCWWGG 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 440
EcoRII CCWGG 2 cut(s) 184, 442
EcoT14I CCWWGG 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 390
FaeI CATG 4 cut(s) 131, 350, 394, 467
FaqI GGGAC 1 cut(s) 371
FatI CATG 4 cut(s) 127, 346, 390, 463
FblI GTMKAC 1 cut(s) 88
FokI GGATG 1 cut(s) 284
FspBI CTAG 2 cut(s) 449, 485
GsuI CTGGAG 1 cut(s) 292
HapII CCGG 2 cut(s) 119, 219
HgaI GACGC 2 cut(s) 224, 448
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 4 cut(s) 131, 350, 394, 467
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 5 cut(s) 152, 195, 232, 358, 384
HpaII CCGG 2 cut(s) 119, 219
Hpy166II GTNNAC 5 cut(s) 89, 227, 241, 366, 481
Hpy188I TCNGA 3 cut(s) 110, 214, 338
Hpy188III TCNNGA 4 cut(s) 79, 128, 281, 320
Hpy8I GTNNAC 5 cut(s) 89, 227, 241, 366, 481
HpyCH4III ACNGT 2 cut(s) 19, 257
HpyCH4V TGCA 3 cut(s) 227, 408, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 272, 318
HpyF3I CTNAG 2 cut(s) 211, 377
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 4 cut(s) 131, 350, 394, 467
Kzo9I GATC 2 cut(s) 300, 326
LmnI GCTCC 1 cut(s) 338
LweI GCATC 2 cut(s) 235, 262
MaeI CTAG 2 cut(s) 449, 485
MaeIII GTNAC 4 cut(s) 13, 143, 251, 432
MalI GATC 2 cut(s) 302, 328
MboI GATC 2 cut(s) 300, 326
MboII GAAGA 1 cut(s) 295
MflI RGATCY 1 cut(s) 326
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 3 cut(s) 61, 104, 417
MlyI GAGTC 2 cut(s) 161, 393
MmeI TCCRAC 1 cut(s) 24
MnlI CCTC 6 cut(s) 67, 215, 277, 287, 352, 513
MslI CAYNNNNRTG 1 cut(s) 474
MspI CCGG 2 cut(s) 119, 219
MspR9I CCNGG 3 cut(s) 119, 186, 444
MvaI CCWGG 2 cut(s) 186, 444
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 3 cut(s) 224, 272, 318
NciI CCSGG 1 cut(s) 119
NcoI CCATGG 1 cut(s) 390
NdeII GATC 2 cut(s) 300, 326
NlaIII CATG 4 cut(s) 131, 350, 394, 467
NlaIV GGNNCC 2 cut(s) 57, 340
NmuCI GTSAC 2 cut(s) 13, 251
NruI TCGCGA 1 cut(s) 321
PagI TCATGA 1 cut(s) 127
PfeI GAWTC 3 cut(s) 195, 232, 358
PfoI TCCNGGA 1 cut(s) 442
PleI GAGTC 2 cut(s) 160, 392
PpsI GAGTC 2 cut(s) 160, 392
PsiI TTATAA 1 cut(s) 35
Psp6I CCWGG 2 cut(s) 184, 442
PspGI CCWGG 2 cut(s) 184, 442
PspN4I GGNNCC 2 cut(s) 57, 340
PspPI GGNCC 1 cut(s) 440
PsuI RGATCY 1 cut(s) 326
RruI TCGCGA 1 cut(s) 321
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
RseI CAYNNNNRTG 1 cut(s) 474
Sau3AI GATC 2 cut(s) 300, 326
Sau96I GGNCC 1 cut(s) 440
SchI GAGTC 2 cut(s) 161, 393
ScrFI CCNGG 3 cut(s) 119, 186, 444
SduI GDGCHC 1 cut(s) 229
SetI ASST 9 cut(s) 78, 88, 187, 288, 314, 378, 476, 486, 490
SfaNI GCATC 2 cut(s) 235, 262
SinI GGWCC 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 474
Sse9I AATT 3 cut(s) 61, 104, 417
SspMI CTAG 2 cut(s) 449, 485
StyD4I CCNGG 3 cut(s) 117, 184, 442
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 2 cut(s) 19, 257
TasI AATT 3 cut(s) 61, 104, 417
TfiI GAWTC 3 cut(s) 195, 232, 358
TscAI CASTG 3 cut(s) 22, 234, 385
TseFI GTSAC 2 cut(s) 13, 251
Tsp45I GTSAC 2 cut(s) 13, 251
TspDTI ATGAA 3 cut(s) 144, 187, 371
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 3 cut(s) 22, 234, 385
VneI GTGCAC 1 cut(s) 225
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 1 cut(s) 61
XmiI GTMKAC 1 cut(s) 88
XspI CTAG 2 cut(s) 449, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.