Rh7AG177400

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0013662
Physical Location & Seq
Forward (+)
15628787 .. 15630932
2146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG177400.1

Sequence Viewer

Length: 849 bp
ATGGCTGACCCAAATACTCCTCCCACTAACTTGCGCCAAATAACAATAGACCAACCCACACCGACCCAGTTCACCTCTGACTTTCCTCCTCCTCCTCCTTCTCCTACACTACACCAGCCCCCTCTTCCAGCTCCATCACATTCTCTTGCTCTTCTTCCTCTTGACCACCCGTGTGGAACACCAATTATACCCATAATACCCCCCACTTCTGAAGCAACAGTAGTACAGCAGCTACAGCTTCAAACACCCAAGGAGGCAACAGCACCGCAGGGAAAGACTCCCATGGCCTCGAGCTCTTCTGGAAAGCACCCGAGGTACAGGGGAATCAGAAGCCGGGGAGGAAAATGGGTGTCGGAAATCCGCGAGCCGCGCAAGACCAAGCGAATATGGCTCGGGACTTTCCCAACTCCTGAGATGGCTGCAGCCGCCTATGACGTGGCGGCACTGGCTCTGAAACGTGAGGATGCTGTTCTCAACTTTCCTAGTTTTATCAAGTCGTATCCAGTACCGGCATCCATGTCTGCCCTTGACATTCGTGCTGCCGCCGCTGCAGCCGCTAGTGCAGTAGGCCCGGCAACGCTGAAAAAGAGTGGCTCCACGGAGACTTCAAATGTTGGACAACAACAGAAAGAGTATGAGGATATGTTGAGTATTAATAACAGTACTTTGGCGTCGTCTGTGGGAGGAGCAGACTTTATGGACGAGGAAGAGATTTTTGGGATGCCGAATTTGCTGGTGGACATGGCTGAGGGAATGCTGGTTTCTCCGCCGAGGATAAACTCGCCGCCTTCTGATTATGACTCGCCGGAGAATTCAGAAGGAGGAGAGAGTCTCTGGAACTACGATTGA

Protein Analysis

282

Amino Acids

30.16

Weight (kDa)

5.08

Isoelectric Point (pI)

59.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 104 - 152 2.2e-11 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 363, 370
AcsI RAATTY 2 cut(s) 727, 811
AcuI CTGAAG 1 cut(s) 231
AcyI GRCGYC 1 cut(s) 671
AfaI GTAC 4 cut(s) 225, 317, 507, 664
AgsI TTSAA 2 cut(s) 242, 609
AjiI CACGTC 1 cut(s) 436
AjuI GAANNNNNNNTTGG 2 cut(s) 699, 731
AleI CACNNNNGTG 1 cut(s) 171
AluBI AGCT 4 cut(s) 131, 232, 238, 294
AluI AGCT 4 cut(s) 131, 232, 238, 294
Alw21I GWGCWC 1 cut(s) 296
Alw26I GTCTC 2 cut(s) 596, 836
Ama87I CYCGRG 3 cut(s) 289, 310, 392
AoxI GGCC 2 cut(s) 285, 568
ApeKI GCWGC 6 cut(s) 229, 419, 422, 539, 548, 551
ApoI RAATTY 2 cut(s) 727, 811
AseI ATTAAT 1 cut(s) 654
AspLEI GCGC 2 cut(s) 36, 372
AspS9I GGNCC 1 cut(s) 569
AsuC2I CCSGG 2 cut(s) 335, 572
AsuHPI GGTGA 1 cut(s) 64
AvaI CYCGRG 3 cut(s) 289, 310, 392
BanII GRGCYC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 296
BbvCI CCTCAGC 1 cut(s) 747
BbvI GCAGC 6 cut(s) 241, 406, 434, 526, 535, 563
BccI CCATC 2 cut(s) 142, 409
BciVI GTATCC 1 cut(s) 510
BcnI CCSGG 2 cut(s) 335, 572
BcoDI GTCTC 2 cut(s) 596, 836
BfaI CTAG 2 cut(s) 483, 558
BfmI CTRYAG 3 cut(s) 233, 420, 549
BfuI GTATCC 1 cut(s) 510
BmcAI AGTACT 1 cut(s) 664
Bme1390I CCNGG 2 cut(s) 335, 572
BmeT110I CYCGRG 3 cut(s) 289, 310, 392
BmgBI CACGTC 1 cut(s) 436
BmgT120I GGNCC 1 cut(s) 569
BmiI GGNNCC 1 cut(s) 595
BmrFI CCNGG 2 cut(s) 335, 572
BmrI ACTGGG 1 cut(s) 61
BmsI GCATC 3 cut(s) 454, 521, 711
BmuI ACTGGG 1 cut(s) 61
BplI GAGNNNNNCTC 2 cut(s) 816, 848
Bpu10I CCTNAGC 1 cut(s) 747
BpuMI CCSGG 2 cut(s) 335, 572
BsaHI GRCGYC 1 cut(s) 671
BsaJI CCNNGG 6 cut(s) 249, 282, 311, 334, 597, 770
Bse118I RCCGGY 1 cut(s) 508
Bse1I ACTGG 3 cut(s) 67, 450, 503
BseDI CCNNGG 6 cut(s) 249, 282, 311, 334, 597, 770
BseGI GGATG 3 cut(s) 469, 512, 726
BseMII CTCAG 2 cut(s) 402, 738
BseNI ACTGG 3 cut(s) 67, 450, 503
BseRI GAGGAG 6 cut(s) 9, 78, 81, 84, 699, 837
BseXI GCAGC 6 cut(s) 241, 406, 434, 526, 535, 563
BsgI GTGCAG 1 cut(s) 582
Bsh1236I CGCG 2 cut(s) 363, 370
BshFI GGCC 2 cut(s) 287, 570
BsiHKAI GWGCWC 1 cut(s) 296
BsiHKCI CYCGRG 3 cut(s) 289, 310, 392
BsiSI CCGG 4 cut(s) 334, 509, 572, 806
BslFI GGGAC 1 cut(s) 409
BsmAI GTCTC 2 cut(s) 596, 836
BsmFI GGGAC 1 cut(s) 409
BsmI GAATGC 1 cut(s) 759
BsnI GGCC 2 cut(s) 287, 570
BsoBI CYCGRG 3 cut(s) 289, 310, 392
Bsp1286I GDGCHC 1 cut(s) 296
Bsp19I CCATGG 1 cut(s) 282
BspANI GGCC 2 cut(s) 287, 570
BspCNI CTCAG 2 cut(s) 403, 739
BspFNI CGCG 2 cut(s) 363, 370
BspLI GGNNCC 1 cut(s) 595
BspMAI CTGCAG 2 cut(s) 424, 553
BspQI GCTCTTC 2 cut(s) 156, 301
BsrFI RCCGGY 1 cut(s) 508
BsrI ACTGG 3 cut(s) 67, 450, 503
BssAI RCCGGY 1 cut(s) 508
BssECI CCNNGG 6 cut(s) 249, 282, 311, 334, 597, 770
BssNI GRCGYC 1 cut(s) 671
BssT1I CCWWGG 2 cut(s) 249, 282
Bst4CI ACNGT 2 cut(s) 220, 662
Bst6I CTCTTC 4 cut(s) 129, 156, 301, 702
BstACI GRCGYC 1 cut(s) 671
BstC8I GCNNGC 1 cut(s) 365
BstDEI CTNAG 2 cut(s) 411, 747
BstDSI CCRYGG 2 cut(s) 282, 597
BstF5I GGATG 3 cut(s) 469, 512, 726
BstFNI CGCG 2 cut(s) 363, 370
BstHHI GCGC 2 cut(s) 36, 372
BstMAI GTCTC 2 cut(s) 596, 836
BstSCI CCNGG 2 cut(s) 333, 570
BstSFI CTRYAG 3 cut(s) 233, 420, 549
BstUI CGCG 2 cut(s) 363, 370
BstV1I GCAGC 6 cut(s) 241, 406, 434, 526, 535, 563
BstXI CCANNNNNNTGG 1 cut(s) 173
BsuI GTATCC 1 cut(s) 510
BsuRI GGCC 2 cut(s) 287, 570
BtgI CCRYGG 2 cut(s) 282, 597
BtrI CACGTC 1 cut(s) 436
BtsCI GGATG 3 cut(s) 469, 512, 726
BtsIMutI CAGTG 1 cut(s) 443
Cac8I GCNNGC 1 cut(s) 365
CfoI GCGC 2 cut(s) 36, 372
Cfr10I RCCGGY 1 cut(s) 508
Cfr13I GGNCC 1 cut(s) 569
CseI GACGC 1 cut(s) 660
Csp6I GTAC 4 cut(s) 224, 316, 506, 663
CviAII CATG 3 cut(s) 283, 517, 742
CviQI GTAC 4 cut(s) 224, 316, 506, 663
DdeI CTNAG 2 cut(s) 411, 747
Eam1104I CTCTTC 4 cut(s) 129, 156, 301, 702
EarI CTCTTC 4 cut(s) 129, 156, 301, 702
EciI GGCGGA 1 cut(s) 756
Ecl136II GAGCTC 1 cut(s) 294
Eco130I CCWWGG 2 cut(s) 249, 282
Eco24I GRGCYC 1 cut(s) 296
Eco53kI GAGCTC 1 cut(s) 294
Eco57I CTGAAG 1 cut(s) 231
Eco88I CYCGRG 3 cut(s) 289, 310, 392
EcoICRI GAGCTC 1 cut(s) 294
EcoRI GAATTC 1 cut(s) 811
EcoT14I CCWWGG 2 cut(s) 249, 282
EcoT38I GRGCYC 1 cut(s) 296
ErhI CCWWGG 2 cut(s) 249, 282
FaeI CATG 3 cut(s) 286, 520, 745
FaqI GGGAC 1 cut(s) 409
FatI CATG 3 cut(s) 282, 516, 741
FokI GGATG 3 cut(s) 476, 499, 733
FriOI GRGCYC 1 cut(s) 296
FspBI CTAG 2 cut(s) 483, 558
GlaI GCGC 2 cut(s) 35, 371
HaeIII GGCC 2 cut(s) 287, 570
HapII CCGG 4 cut(s) 334, 509, 572, 806
HgaI GACGC 1 cut(s) 660
HhaI GCGC 2 cut(s) 36, 372
Hin1I GRCGYC 1 cut(s) 671
Hin1II CATG 3 cut(s) 286, 520, 745
Hin6I GCGC 2 cut(s) 34, 370
HinP1I GCGC 2 cut(s) 34, 370
HinfI GANTC 4 cut(s) 277, 324, 800, 829
HpaII CCGG 4 cut(s) 334, 509, 572, 806
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 2 cut(s) 72, 739
Hpy188I TCNGA 7 cut(s) 79, 211, 329, 355, 453, 793, 817
Hpy188III TCNNGA 5 cut(s) 161, 300, 394, 410, 835
Hpy8I GTNNAC 2 cut(s) 72, 739
Hpy99I CGWCG 1 cut(s) 676
HpyAV CCTTC 3 cut(s) 108, 798, 812
HpyCH4III ACNGT 2 cut(s) 220, 662
HpyCH4IV ACGT 2 cut(s) 435, 457
HpyCH4V TGCA 3 cut(s) 422, 551, 563
HpyF3I CTNAG 2 cut(s) 411, 747
HpySE526I ACGT 2 cut(s) 435, 457
Hsp92I GRCGYC 1 cut(s) 671
Hsp92II CATG 3 cut(s) 286, 520, 745
HspAI GCGC 2 cut(s) 34, 370
LguI GCTCTTC 2 cut(s) 156, 301
LmnI GCTCC 3 cut(s) 136, 599, 686
Lsp1109I GCAGC 6 cut(s) 241, 406, 434, 526, 535, 563
LweI GCATC 3 cut(s) 454, 521, 711
MaeI CTAG 2 cut(s) 483, 558
MaeII ACGT 2 cut(s) 435, 457
MboII GAAGA 5 cut(s) 116, 143, 146, 288, 719
MhlI GDGCHC 1 cut(s) 296
MluCI AATT 3 cut(s) 183, 727, 811
MlyI GAGTC 3 cut(s) 271, 794, 838
MmeI TCCRAC 2 cut(s) 333, 595
MseI TTAA 1 cut(s) 654
MslI CAYNNNNRTG 1 cut(s) 171
MspA1I CMGCKG 1 cut(s) 548
MspI CCGG 4 cut(s) 334, 509, 572, 806
MspR9I CCNGG 2 cut(s) 335, 572
Mva1269I GAATGC 1 cut(s) 759
MvnI CGCG 2 cut(s) 363, 370
NciI CCSGG 2 cut(s) 335, 572
NcoI CCATGG 1 cut(s) 282
NlaIII CATG 3 cut(s) 286, 520, 745
NlaIV GGNNCC 1 cut(s) 595
NmeAIII GCCGAG 1 cut(s) 795
OliI CACNNNNGTG 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 289
PciSI GCTCTTC 2 cut(s) 156, 301
PctI GAATGC 1 cut(s) 759
PfeI GAWTC 1 cut(s) 324
PleI GAGTC 3 cut(s) 271, 794, 837
PpsI GAGTC 3 cut(s) 271, 794, 837
PshBI ATTAAT 1 cut(s) 654
Psp124BI GAGCTC 1 cut(s) 296
PspN4I GGNNCC 1 cut(s) 595
PspPI GGNCC 1 cut(s) 569
PspXI VCTCGAGB 1 cut(s) 289
PstI CTGCAG 2 cut(s) 424, 553
RsaI GTAC 4 cut(s) 225, 317, 507, 664
RsaNI GTAC 4 cut(s) 224, 316, 506, 663
RseI CAYNNNNRTG 1 cut(s) 171
SacI GAGCTC 1 cut(s) 296
SapI GCTCTTC 2 cut(s) 156, 301
SaqAI TTAA 1 cut(s) 654
Sau96I GGNCC 1 cut(s) 569
ScaI AGTACT 1 cut(s) 664
SchI GAGTC 3 cut(s) 271, 794, 838
ScrFI CCNGG 2 cut(s) 335, 572
SduI GDGCHC 1 cut(s) 296
SetI ASST 8 cut(s) 77, 133, 234, 240, 296, 317, 438, 460
SfaNI GCATC 3 cut(s) 454, 521, 711
SfcI CTRYAG 3 cut(s) 233, 420, 549
Sfr274I CTCGAG 1 cut(s) 289
SlaI CTCGAG 1 cut(s) 289
SmiMI CAYNNNNRTG 1 cut(s) 171
SmlI CTYRAG 1 cut(s) 289
SmoI CTYRAG 1 cut(s) 289
Sse9I AATT 3 cut(s) 183, 727, 811
SspMI CTAG 2 cut(s) 483, 558
SstI GAGCTC 1 cut(s) 296
StyD4I CCNGG 2 cut(s) 333, 570
StyI CCWWGG 2 cut(s) 249, 282
TaaI ACNGT 2 cut(s) 220, 662
TaiI ACGT 2 cut(s) 438, 460
TaqI TCGA 1 cut(s) 290
TasI AATT 3 cut(s) 183, 727, 811
TatI WGTACW 2 cut(s) 223, 662
TauI GCSGC 7 cut(s) 370, 428, 443, 545, 548, 557, 787
TfiI GAWTC 1 cut(s) 324
Tru1I TTAA 1 cut(s) 654
Tru9I TTAA 1 cut(s) 654
TscAI CASTG 1 cut(s) 450
TseI GCWGC 6 cut(s) 229, 419, 422, 539, 548, 551
TspGWI ACGGA 1 cut(s) 614
TspRI CASTG 1 cut(s) 450
VspI ATTAAT 1 cut(s) 654
XapI RAATTY 2 cut(s) 727, 811
XhoI CTCGAG 1 cut(s) 289
XspI CTAG 2 cut(s) 483, 558
ZrmI AGTACT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.