Rh7CG186600

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0013662
Physical Location & Seq
Forward (+)
16349645 .. 16350723
1079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG186600.1

Sequence Viewer

Length: 570 bp
ATGGCCTCGAGCTCTTCTGGAAAGCACCCGAGGTACCGGGGAATCCGAAGCCGGGGAGGAAAATGGGTGTCGGAAATCCGCGAGCCGCGCAAGACCAAGCGAATATGGCTCGGGACTTTCCCAACTCCTGAGATGGCTGCAGCCGCCTATGACGTGGCGGCACTGGCTCTGAAACGTGAGGATGCTGTTCTCAACTTTCCTAATTTTATCAAGTCGTATCCAGTACCGGCATCCATGTCTGCCCTTGACATTCGTGCTGCCGCCGCTGCAGCCGCTAGTGCAGTAGGCCCGGCAACGCTGAAAAAGAGTGGCTCCACGGAGACTTCAAATGTTGGACAACAACAGAAAGAGTATGAGGATATGTTGAGTATTAATAACAGTACTTTGGAGTCGTCTGTGGGAGCAGACTTTATGGACGAGGAAGAGATTTTTGGGATGCCGAATTTGCTGGTGGACATGGCTGAGGGAATGCTGGTTTCTCCGCCGAGGATAAACTCGCCGCCTTCTGATTATGACTCGCCGGAGAATTCAGAAGGAGGAGGAGGAGAGAGTCTCTGGAACTACGATTGA

Protein Analysis

189

Amino Acids

20.34

Weight (kDa)

4.94

Isoelectric Point (pI)

59.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 10 - 58 1.1e-11 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 33
AccB1I GGYRCC 1 cut(s) 33
AccII CGCG 2 cut(s) 81, 88
AciI CCGC 9 cut(s) 79, 86, 144, 158, 261, 264, 273, 482, 500
AcsI RAATTY 2 cut(s) 442, 526
AfaI GTAC 3 cut(s) 35, 225, 382
AfiI CCNNNNNNNGG 1 cut(s) 52
AgsI TTSAA 1 cut(s) 327
AjiI CACGTC 1 cut(s) 154
AjuI GAANNNNNNNTTGG 2 cut(s) 414, 446
AluBI AGCT 1 cut(s) 12
AluI AGCT 1 cut(s) 12
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 2 cut(s) 314, 557
Ama87I CYCGRG 3 cut(s) 7, 28, 110
AoxI GGCC 2 cut(s) 3, 286
ApeKI GCWGC 5 cut(s) 137, 140, 257, 266, 269
ApoI RAATTY 2 cut(s) 442, 526
AseI ATTAAT 1 cut(s) 372
Asp718I GGTACC 1 cut(s) 33
AspLEI GCGC 1 cut(s) 90
AspS9I GGNCC 1 cut(s) 287
AsuC2I CCSGG 3 cut(s) 38, 53, 290
AvaI CYCGRG 3 cut(s) 7, 28, 110
BanI GGYRCC 1 cut(s) 33
BanII GRGCYC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 14
BbvCI CCTCAGC 1 cut(s) 462
BbvI GCAGC 5 cut(s) 124, 152, 244, 253, 281
BccI CCATC 1 cut(s) 127
BciVI GTATCC 1 cut(s) 228
BcnI CCSGG 3 cut(s) 38, 53, 290
BcoDI GTCTC 2 cut(s) 314, 557
BfaI CTAG 1 cut(s) 276
BfmI CTRYAG 2 cut(s) 138, 267
BfuI GTATCC 1 cut(s) 228
BmcAI AGTACT 1 cut(s) 382
Bme1390I CCNGG 3 cut(s) 38, 53, 290
BmeT110I CYCGRG 3 cut(s) 7, 28, 110
BmgBI CACGTC 1 cut(s) 154
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 2 cut(s) 35, 313
BmrFI CCNGG 3 cut(s) 38, 53, 290
BmsI GCATC 3 cut(s) 172, 239, 426
BplI GAGNNNNNCTC 2 cut(s) 537, 569
Bpu10I CCTNAGC 1 cut(s) 462
BpuMI CCSGG 3 cut(s) 38, 53, 290
BsaJI CCNNGG 5 cut(s) 29, 37, 52, 315, 485
Bsc4I CCNNNNNNNGG 1 cut(s) 52
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 2 cut(s) 168, 221
BseDI CCNNGG 5 cut(s) 29, 37, 52, 315, 485
BseGI GGATG 3 cut(s) 187, 230, 441
BseLI CCNNNNNNNGG 1 cut(s) 52
BseMII CTCAG 2 cut(s) 120, 453
BseNI ACTGG 2 cut(s) 168, 221
BseRI GAGGAG 3 cut(s) 552, 555, 558
BseXI GCAGC 5 cut(s) 124, 152, 244, 253, 281
BsgI GTGCAG 1 cut(s) 300
Bsh1236I CGCG 2 cut(s) 81, 88
BshFI GGCC 2 cut(s) 5, 288
BshNI GGYRCC 1 cut(s) 33
BsiHKAI GWGCWC 1 cut(s) 14
BsiHKCI CYCGRG 3 cut(s) 7, 28, 110
BsiSI CCGG 5 cut(s) 37, 52, 227, 290, 521
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 1 cut(s) 52
BsmAI GTCTC 2 cut(s) 314, 557
BsmFI GGGAC 1 cut(s) 127
BsmI GAATGC 1 cut(s) 474
BsnI GGCC 2 cut(s) 5, 288
BsoBI CYCGRG 3 cut(s) 7, 28, 110
Bsp1286I GDGCHC 1 cut(s) 14
BspACI CCGC 9 cut(s) 79, 86, 144, 158, 261, 264, 273, 482, 500
BspANI GGCC 2 cut(s) 5, 288
BspCNI CTCAG 2 cut(s) 121, 454
BspFNI CGCG 2 cut(s) 81, 88
BspLI GGNNCC 2 cut(s) 35, 313
BspMAI CTGCAG 2 cut(s) 142, 271
BspQI GCTCTTC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 33
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 2 cut(s) 168, 221
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 5 cut(s) 29, 37, 52, 315, 485
Bst4CI ACNGT 1 cut(s) 380
Bst6I CTCTTC 2 cut(s) 19, 417
BstC8I GCNNGC 1 cut(s) 83
BstDEI CTNAG 2 cut(s) 129, 462
BstDSI CCRYGG 1 cut(s) 315
BstF5I GGATG 3 cut(s) 187, 230, 441
BstFNI CGCG 2 cut(s) 81, 88
BstHHI GCGC 1 cut(s) 90
BstMAI GTCTC 2 cut(s) 314, 557
BstSCI CCNGG 3 cut(s) 36, 51, 288
BstSFI CTRYAG 2 cut(s) 138, 267
BstUI CGCG 2 cut(s) 81, 88
BstV1I GCAGC 5 cut(s) 124, 152, 244, 253, 281
BsuI GTATCC 1 cut(s) 228
BsuRI GGCC 2 cut(s) 5, 288
BtgI CCRYGG 1 cut(s) 315
BtrI CACGTC 1 cut(s) 154
BtsCI GGATG 3 cut(s) 187, 230, 441
BtsIMutI CAGTG 1 cut(s) 161
Cac8I GCNNGC 1 cut(s) 83
CfoI GCGC 1 cut(s) 90
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 1 cut(s) 287
Csp6I GTAC 3 cut(s) 34, 224, 381
CviAII CATG 2 cut(s) 235, 457
CviQI GTAC 3 cut(s) 34, 224, 381
DdeI CTNAG 2 cut(s) 129, 462
Eam1104I CTCTTC 2 cut(s) 19, 417
EarI CTCTTC 2 cut(s) 19, 417
EciI GGCGGA 1 cut(s) 471
Ecl136II GAGCTC 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 14
Eco53kI GAGCTC 1 cut(s) 12
Eco88I CYCGRG 3 cut(s) 7, 28, 110
EcoICRI GAGCTC 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 526
EcoT38I GRGCYC 1 cut(s) 14
FaeI CATG 2 cut(s) 238, 460
FaiI YATR 8 cut(s) 106, 150, 236, 354, 362, 413, 458, 513
FaqI GGGAC 1 cut(s) 127
FatI CATG 2 cut(s) 234, 456
FokI GGATG 3 cut(s) 194, 217, 448
FriOI GRGCYC 1 cut(s) 14
FspBI CTAG 1 cut(s) 276
GlaI GCGC 1 cut(s) 89
HaeIII GGCC 2 cut(s) 5, 288
HapII CCGG 5 cut(s) 37, 52, 227, 290, 521
HhaI GCGC 1 cut(s) 90
Hin1II CATG 2 cut(s) 238, 460
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HinfI GANTC 4 cut(s) 42, 389, 515, 550
HpaII CCGG 5 cut(s) 37, 52, 227, 290, 521
Hpy166II GTNNAC 1 cut(s) 454
Hpy188I TCNGA 5 cut(s) 47, 73, 171, 508, 532
Hpy188III TCNNGA 4 cut(s) 18, 112, 128, 556
Hpy8I GTNNAC 1 cut(s) 454
HpyAV CCTTC 2 cut(s) 513, 527
HpyCH4III ACNGT 1 cut(s) 380
HpyCH4IV ACGT 2 cut(s) 153, 175
HpyCH4V TGCA 3 cut(s) 140, 269, 281
HpyF3I CTNAG 2 cut(s) 129, 462
HpySE526I ACGT 2 cut(s) 153, 175
Hsp92II CATG 2 cut(s) 238, 460
HspAI GCGC 1 cut(s) 88
KpnI GGTACC 1 cut(s) 37
LguI GCTCTTC 1 cut(s) 19
LmnI GCTCC 2 cut(s) 317, 401
Lsp1109I GCAGC 5 cut(s) 124, 152, 244, 253, 281
LweI GCATC 3 cut(s) 172, 239, 426
MaeI CTAG 1 cut(s) 276
MaeII ACGT 2 cut(s) 153, 175
MboII GAAGA 2 cut(s) 6, 434
MhlI GDGCHC 1 cut(s) 14
MluCI AATT 3 cut(s) 202, 442, 526
MlyI GAGTC 3 cut(s) 398, 509, 559
MmeI TCCRAC 2 cut(s) 51, 313
MseI TTAA 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 5 cut(s) 37, 52, 227, 290, 521
MspR9I CCNGG 3 cut(s) 38, 53, 290
Mva1269I GAATGC 1 cut(s) 474
MvnI CGCG 2 cut(s) 81, 88
NciI CCSGG 3 cut(s) 38, 53, 290
NlaIII CATG 2 cut(s) 238, 460
NlaIV GGNNCC 2 cut(s) 35, 313
NmeAIII GCCGAG 1 cut(s) 510
PaeR7I CTCGAG 1 cut(s) 7
PciSI GCTCTTC 1 cut(s) 19
PctI GAATGC 1 cut(s) 474
PfeI GAWTC 1 cut(s) 42
PleI GAGTC 3 cut(s) 397, 509, 558
PpsI GAGTC 3 cut(s) 397, 509, 558
PshBI ATTAAT 1 cut(s) 372
Psp124BI GAGCTC 1 cut(s) 14
PspN4I GGNNCC 2 cut(s) 35, 313
PspPI GGNCC 1 cut(s) 287
PspXI VCTCGAGB 1 cut(s) 7
PstI CTGCAG 2 cut(s) 142, 271
RsaI GTAC 3 cut(s) 35, 225, 382
RsaNI GTAC 3 cut(s) 34, 224, 381
SacI GAGCTC 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 19
SaqAI TTAA 1 cut(s) 372
Sau96I GGNCC 1 cut(s) 287
ScaI AGTACT 1 cut(s) 382
SchI GAGTC 3 cut(s) 398, 509, 559
ScrFI CCNGG 3 cut(s) 38, 53, 290
SduI GDGCHC 1 cut(s) 14
SetI ASST 4 cut(s) 14, 35, 156, 178
SfaNI GCATC 3 cut(s) 172, 239, 426
SfcI CTRYAG 2 cut(s) 138, 267
Sfr274I CTCGAG 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 7
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
Sse9I AATT 3 cut(s) 202, 442, 526
SsiI CCGC 9 cut(s) 79, 86, 144, 158, 261, 264, 273, 482, 500
SspMI CTAG 1 cut(s) 276
SstI GAGCTC 1 cut(s) 14
StyD4I CCNGG 3 cut(s) 36, 51, 288
TaaI ACNGT 1 cut(s) 380
TaiI ACGT 2 cut(s) 156, 178
TaqI TCGA 1 cut(s) 8
TasI AATT 3 cut(s) 202, 442, 526
TatI WGTACW 1 cut(s) 380
TauI GCSGC 7 cut(s) 88, 146, 161, 263, 266, 275, 502
TfiI GAWTC 1 cut(s) 42
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 1 cut(s) 168
TseI GCWGC 5 cut(s) 137, 140, 257, 266, 269
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 1 cut(s) 168
VspI ATTAAT 1 cut(s) 372
XapI RAATTY 2 cut(s) 442, 526
XhoI CTCGAG 1 cut(s) 7
XspI CTAG 1 cut(s) 276
ZrmI AGTACT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.