Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00751

Orthogroup ID

OG0001090

Physical Location & Seq

Genomic Coordinates Reverse (-)

336552 .. 338880

Gene Structure 2329 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G016480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1251 bp

ATGATTGGAATTTTTCGAGTGAGGTTAAAGATGTATGTGGTGGATAGCAAAGGAGGTGCTATAGCATGCATGCTGCTGGCACTGTTCTTATTGGGGACATGGCCTGCTGTCCTGACTATGCTCGAAAGAAGAGGCCGGCTTCCTCAGCATACTTATTTGGATTACTCTATCACCAATTTGTTGGTTGCTGTTCTGATTGCTTTGACATTTGGTCAGATAGGCAGTAGCACACCTGACAAGCCCAATTTTATAACTCAACTTTCACAGGATAATTTGCCTAGCGTTTTATTCGCAATGGCGGGTGGGATAGTCCTGAGCCTTGGAAATCTTTCTTCACAGTATGCATTTGCTTTAGTTGGTTTGTCACTGACAGAGGTGATCACTGCAAGCATCACAACTGTCATAGGCACAACCTTGAATTACTTCTTAGACAACAGAATTAATAGAGCCGAGATTCTTTTCCCTGGTGTTGCTTGCTTCTTGATTGCGGTTTGTCTTGGCTCTGCTGTTCACTCGTCCAACGCAGCTGATAACAAAGTAAAACTTGACAGTTTTCCCAGCGATTCAAAAGATGGAGAAAATTGTAACATTCTAGTATATGCTTGTCTGGTTGCTCCTTATGACATATATAATGGGTTAAAGGATTTGGAGAGTGGAAATGAGAAAGCGAAGGCTGGCACTGCAGACTATCTTGTACAGATTGAGAACCAAAGAGCAATTAAGGTTTTTGGGAAGAAGACTTACATTGGACTGGCCATTACTTTCTTTGCGGGACTTTGCTTCTCCCTCTTCACACCAGCATTCAACGTGGCCACAAATGATCAGTGGCATACTTTGAAGAAAGGAGTTCCTCATTTGGTTGTCTATACTGCATTTTTCTACTTCTCAGCATCTTGTTTCGTCATTGCTATCATTTTAAACACCATCTTCCTTTACCGCCCTATACTAGACTTACCCAAAAGTTCATTCAAAGCTTACTTGAACGACTGGGAGGGTAGGGGTTGGGCCCTTTTGGCTGGCCTCATATGTGGATTCGGCAACGGTCTCCAATTCATGGGAGGTCAAGCTGCAGGATATGCTGCAGCTGATTCTGTTCAGGCACTTCCACTTGTGAGCACTTTCTGGGGCATACTTCTGTTTGGAGAATACCGAAAATCGTCACGAAGAACATATATATTGCTTACTAGCATGTTGACTATGTTTATTGTTGCTGTTGCTGTCCTCATGGCATCATCGGGGCATCGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005274 GO:0005345 GO:0005350 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006206 GO:0006725 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006855 GO:0006863 GO:0008150 GO:0008152 GO:0008324 GO:0008655 GO:0009058 GO:0009112 GO:0009507 GO:0009536 GO:0009987 GO:0015075 GO:0015077 GO:0015078 GO:0015205 GO:0015210 GO:0015238 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015672 GO:0015720 GO:0015851 GO:0015855 GO:0015857 GO:0015893 GO:0016020 GO:0018130 GO:0019438 GO:0019856 GO:0022804 GO:0022857 GO:0022890 GO:0034220 GO:0034641 GO:0034654 GO:0042221 GO:0042493 GO:0042886 GO:0042887 GO:0042906 GO:0042907 GO:0043094 GO:0043100 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046112 GO:0046483 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0055086 GO:0071702 GO:0071704 GO:0071705 GO:0071944 GO:0072527 GO:0072528 GO:0072530 GO:0072531 GO:0098655 GO:0098660 GO:0098662 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600 GO:1903791 GO:1904082 GO:1904823

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ureide_permease

Protein Analysis

416

Amino Acids

45.28

Weight (kDa)

8.89

Isoelectric Point (pI)

33.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ureide_permease	PF07168	51 - 411	7.5e-191	Ureide permease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001090)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26440 AT1G26440 AT1G26440 AT1G26440 AT1G26440 AT1G26440 AT1G26440 AT1G26440 AT2G03520 AT2G03530 AT2G03530 AT2G03530 AT2G03530 AT2G03530 AT2G03590 AT2G03590 AT2G03600 AT2G03600 AT2G03600 AT2G03600
fragaria_vesca	FvH4_4g30100 FvH4_4g30100 FvH4_4g30101 FvH4_4g30101 FvH4_4g30101 FvH4_4g30102
malus_domestica	MD16G1061500.v1.1
prunus_persica	Prupe.1G286600_v2.0.a1 Prupe.1G286600_v2.0.a1 Prupe.1G286600_v2.0.a1
pyrus_communis	pycom16g05370
rosa_chinensis	RchiOBHm_Chr4g0438401 RchiOBHm_Chr4g0438411 RchiOBHm_Chr4g0438431
rosa_laevigata	RLG00000006366 RLG00000006367
rosa_multiflora	Rmu_co8316253.1_g000001 Rmu_sc0002327.1_g000024 Rmu_sc0002327.1_g000025 Rmu_sc0019260.1_g000004 Rmu_sc0021561.1_g000001
rosa_roxburghii	Rroxscaffold_5G00379430 Rroxscaffold_5G00379440
rosa_rugosa	Rorug04G0309700 Rorug04G0309800
rosa_samantha	Rh4AG360500 Rh4BG371400 Rh4BG371500 Rh4CG385500 Rh4CG385600 Rh4DG366400 Rh4DG366500 Rh4DG366600
rosa_wichuraiana	Rw0G006510 Rw0G006520 Rw0G006530 Rw0G016460 Rw0G016470 Rw0G016480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	251
AccB7I	CCANNNNNTGG	1 cut(s)	1054
AciI	CCGC	4 cut(s)	299, 488, 770, 937
AcoI	YGGCCR	2 cut(s)	753, 810
AcsI	RAATTY	1 cut(s)	9
AfaI	GTAC	1 cut(s)	696
AfiI	CCNNNNNNNGG	1 cut(s)	1054
AgsI	TTSAA	6 cut(s)	418, 567, 805, 838, 970, 982
AhdI	GACNNNNNGTC	1 cut(s)	210
AjnI	CCWGG	1 cut(s)	463
AjuI	GAANNNNNNNTTGG	2 cut(s)	950, 982
AluBI	AGCT	4 cut(s)	527, 974, 1067, 1085
AluI	AGCT	4 cut(s)	527, 974, 1067, 1085
Alw21I	GWGCWC	1 cut(s)	1118
Alw26I	GTCTC	1 cut(s)	1049
AoxI	GGCC	6 cut(s)	101, 133, 753, 810, 1005, 1018
ApaI	GGGCCC	1 cut(s)	1009
ApeKI	GCWGC	5 cut(s)	73, 524, 1067, 1079, 1082
ApoI	RAATTY	1 cut(s)	9
AseI	ATTAAT	1 cut(s)	441
Asp700I	GAANNNNTTC	2 cut(s)	328, 422
AspS9I	GGNCC	2 cut(s)	1005, 1006
AsuHPI	GGTGA	2 cut(s)	163, 388
BaeGI	GKGCMC	1 cut(s)	1009
BalI	TGGCCA	2 cut(s)	755, 812
BanII	GRGCYC	1 cut(s)	1009
BarI	GAAGNNNNNNTAC	2 cut(s)	725, 757
BbsI	GAAGAC	1 cut(s)	743
Bbv12I	GWGCWC	1 cut(s)	1118
BbvCI	CCTCAGC	1 cut(s)	144
BbvI	GCAGC	5 cut(s)	60, 536, 1054, 1066, 1094
BccI	CCATC	2 cut(s)	566, 932
BciT130I	CCWGG	1 cut(s)	465
BclI	TGATCA	2 cut(s)	378, 820
BcoDI	GTCTC	1 cut(s)	1049
BfaI	CTAG	4 cut(s)	279, 593, 947, 1185
BfmI	CTRYAG	4 cut(s)	60, 681, 1068, 1080
BglI	GCCNNNNNGGC	1 cut(s)	1013
BisI	GCNGC	5 cut(s)	74, 525, 1068, 1080, 1083
BlsI	GCNGC	5 cut(s)	75, 526, 1069, 1081, 1084
Bme1390I	CCNGG	1 cut(s)	465
BmeRI	GACNNNNNGTC	1 cut(s)	210
BmgT120I	GGNCC	2 cut(s)	1005, 1006
BmiI	GGNNCC	1 cut(s)	1007
BmrFI	CCNGG	1 cut(s)	465
BmrI	ACTGGG	1 cut(s)	997
BmsI	GCATC	3 cut(s)	399, 899, 1238
BmuI	ACTGGG	1 cut(s)	997
BpiI	GAAGAC	1 cut(s)	743
Bpu10I	CCTNAGC	2 cut(s)	144, 314
BsaI	GGTCTC	1 cut(s)	1049
BsaJI	CCNNGG	2 cut(s)	319, 463
Bsc4I	CCNNNNNNNGG	1 cut(s)	1054
Bse118I	RCCGGY	1 cut(s)	135
Bse1I	ACTGG	2 cut(s)	756, 992
Bse3DI	GCAATG	2 cut(s)	300, 903
BseBI	CCWGG	1 cut(s)	465
BseDI	CCNNGG	2 cut(s)	319, 463
BseLI	CCNNNNNNNGG	1 cut(s)	1054
BseMI	GCAATG	2 cut(s)	300, 903
BseMII	CTCAG	3 cut(s)	158, 305, 900
BseNI	ACTGG	2 cut(s)	756, 992
BseSI	GKGCMC	1 cut(s)	1009
BseXI	GCAGC	5 cut(s)	60, 536, 1054, 1066, 1094
BseYI	CCCAGC	1 cut(s)	557
BshFI	GGCC	6 cut(s)	103, 135, 755, 812, 1007, 1020
BsiHKAI	GWGCWC	1 cut(s)	1118
BsiSI	CCGG	1 cut(s)	136
BslFI	GGGAC	2 cut(s)	109, 786
BslI	CCNNNNNNNGG	1 cut(s)	1054
BsmAI	GTCTC	1 cut(s)	1049
BsmFI	GGGAC	2 cut(s)	109, 786
BsmI	GAATGC	1 cut(s)	800
BsnI	GGCC	6 cut(s)	103, 135, 755, 812, 1007, 1020
Bso31I	GGTCTC	1 cut(s)	1049
Bsp120I	GGGCCC	1 cut(s)	1005
Bsp1286I	GDGCHC	2 cut(s)	1009, 1118
Bsp1407I	TGTACA	1 cut(s)	694
Bsp143I	GATC	2 cut(s)	378, 820
BspACI	CCGC	4 cut(s)	299, 488, 770, 937
BspANI	GGCC	6 cut(s)	103, 135, 755, 812, 1007, 1020
BspCNI	CTCAG	3 cut(s)	157, 306, 899
BspLI	GGNNCC	1 cut(s)	1007
BspMAI	CTGCAG	3 cut(s)	685, 1072, 1084
BspTNI	GGTCTC	1 cut(s)	1049
BsrDI	GCAATG	2 cut(s)	300, 903
BsrFI	RCCGGY	1 cut(s)	135
BsrGI	TGTACA	1 cut(s)	694
BsrI	ACTGG	2 cut(s)	756, 992
BssAI	RCCGGY	1 cut(s)	135
BssECI	CCNNGG	2 cut(s)	319, 463
BssMI	GATC	2 cut(s)	378, 820
BssT1I	CCWWGG	1 cut(s)	319
Bst2UI	CCWGG	1 cut(s)	465
Bst4CI	ACNGT	5 cut(s)	84, 339, 400, 551, 1043
Bst6I	CTCTTC	2 cut(s)	124, 794
BstAPI	GCANNNNNTGC	1 cut(s)	1076
BstAUI	TGTACA	1 cut(s)	694
BstC8I	GCNNGC	9 cut(s)	67, 71, 78, 105, 137, 388, 475, 676, 1018
BstDEI	CTNAG	4 cut(s)	144, 314, 427, 886
BstKTI	GATC	2 cut(s)	381, 823
BstMAI	GTCTC	1 cut(s)	1049
BstMBI	GATC	2 cut(s)	378, 820
BstMWI	GCNNNNNNNGC	4 cut(s)	145, 680, 1013, 1076
BstNI	CCWGG	1 cut(s)	465
BstNSI	RCATGY	3 cut(s)	69, 73, 1192
BstSCI	CCNGG	1 cut(s)	463
BstSFI	CTRYAG	4 cut(s)	60, 681, 1068, 1080
BstSLI	GKGCMC	1 cut(s)	1009
BstV1I	GCAGC	5 cut(s)	60, 536, 1054, 1066, 1094
BstV2I	GAAGAC	1 cut(s)	743
BstXI	CCANNNNNNTGG	1 cut(s)	181
BsuRI	GGCC	6 cut(s)	103, 135, 755, 812, 1007, 1020
BtsI	GCAGTG	2 cut(s)	381, 678
BtsIMutI	CAGTG	5 cut(s)	80, 365, 381, 678, 830
Cac8I	GCNNGC	9 cut(s)	67, 71, 78, 105, 137, 388, 475, 676, 1018
Cfr10I	RCCGGY	1 cut(s)	135
Cfr13I	GGNCC	2 cut(s)	1005, 1006
Csp6I	GTAC	1 cut(s)	695
CviAII	CATG	6 cut(s)	66, 70, 99, 1054, 1189, 1225
CviQI	GTAC	1 cut(s)	695
DdeI	CTNAG	4 cut(s)	144, 314, 427, 886
DpnI	GATC	2 cut(s)	380, 822
DpnII	GATC	2 cut(s)	378, 820
DraI	TTTAAA	1 cut(s)	918
DriI	GACNNNNNGTC	1 cut(s)	210
EaeI	YGGCCR	2 cut(s)	753, 810
Eam1104I	CTCTTC	2 cut(s)	124, 794
Eam1105I	GACNNNNNGTC	1 cut(s)	210
EarI	CTCTTC	2 cut(s)	124, 794
Eco130I	CCWWGG	1 cut(s)	319
Eco24I	GRGCYC	1 cut(s)	1009
Eco31I	GGTCTC	1 cut(s)	1049
EcoO109I	RGGNCCY	1 cut(s)	1006
EcoRII	CCWGG	1 cut(s)	463
EcoT14I	CCWWGG	1 cut(s)	319
EcoT22I	ATGCAT	2 cut(s)	71, 346
EcoT38I	GRGCYC	1 cut(s)	1009
ErhI	CCWWGG	1 cut(s)	319
FaeI	CATG	6 cut(s)	69, 73, 102, 1057, 1192, 1228
FalI	AAGNNNNNCTT	2 cut(s)	528, 560
FaqI	GGGAC	2 cut(s)	109, 786
FatI	CATG	6 cut(s)	65, 69, 98, 1053, 1188, 1224
FauI	CCCGC	2 cut(s)	292, 763
FauNDI	CATATG	1 cut(s)	1025
FbaI	TGATCA	2 cut(s)	378, 820
Fnu4HI	GCNGC	5 cut(s)	74, 525, 1068, 1080, 1083
FriOI	GRGCYC	1 cut(s)	1009
Fsp4HI	GCNGC	5 cut(s)	74, 525, 1068, 1080, 1083
FspBI	CTAG	4 cut(s)	279, 593, 947, 1185
GluI	GCNGC	5 cut(s)	74, 525, 1068, 1080, 1083
GsaI	CCCAGC	1 cut(s)	561
HaeIII	GGCC	6 cut(s)	103, 135, 755, 812, 1007, 1020
HapII	CCGG	1 cut(s)	136
Hin1II	CATG	6 cut(s)	69, 73, 102, 1057, 1192, 1228
HincII	GTYRAC	1 cut(s)	1194
HindII	GTYRAC	1 cut(s)	1194
HindIII	AAGCTT	1 cut(s)	972
HinfI	GANTC	4 cut(s)	454, 563, 1032, 1088
HpaII	CCGG	1 cut(s)	136
HphI	GGTGA	2 cut(s)	163, 388
Hpy166II	GTNNAC	2 cut(s)	511, 1194
Hpy188I	TCNGA	2 cut(s)	195, 216
Hpy188III	TCNNGA	4 cut(s)	112, 313, 481, 1161
Hpy8I	GTNNAC	2 cut(s)	511, 1194
HpyAV	CCTTC	1 cut(s)	664
HpyCH4III	ACNGT	5 cut(s)	84, 339, 400, 551, 1043
HpyCH4IV	ACGT	1 cut(s)	807
HpyCH4V	TGCA	7 cut(s)	69, 344, 386, 683, 872, 1070, 1082
HpyF10VI	GCNNNNNNNGC	4 cut(s)	145, 680, 1013, 1076
HpyF3I	CTNAG	4 cut(s)	144, 314, 427, 886
HpySE526I	ACGT	1 cut(s)	807
Hsp92II	CATG	6 cut(s)	69, 73, 102, 1057, 1192, 1228
KroI	GCCGGC	1 cut(s)	135
KroNI	GCCGGC	1 cut(s)	137
Ksp22I	TGATCA	2 cut(s)	378, 820
Kzo9I	GATC	2 cut(s)	378, 820
LmnI	GCTCC	1 cut(s)	619
Lsp1109I	GCAGC	5 cut(s)	60, 536, 1054, 1066, 1094
LweI	GCATC	3 cut(s)	399, 899, 1238
MaeI	CTAG	4 cut(s)	279, 593, 947, 1185
MaeII	ACGT	1 cut(s)	807
MaeIII	GTNAC	3 cut(s)	363, 584, 1158
MalI	GATC	2 cut(s)	380, 822
MboI	GATC	2 cut(s)	378, 820
MboII	GAAGA	8 cut(s)	141, 324, 745, 748, 781, 850, 919, 1176
MhlI	GDGCHC	2 cut(s)	1009, 1118
MlsI	TGGCCA	2 cut(s)	755, 812
MluCI	AATT	9 cut(s)	9, 175, 244, 271, 418, 438, 580, 717, 1049
MluNI	TGGCCA	2 cut(s)	755, 812
MmeI	TCCRAC	1 cut(s)	543
Mox20I	TGGCCA	2 cut(s)	755, 812
Mph1103I	ATGCAT	2 cut(s)	71, 346
MroNI	GCCGGC	1 cut(s)	135
MroXI	GAANNNNTTC	2 cut(s)	328, 422
MscI	TGGCCA	2 cut(s)	755, 812
MseI	TTAA	5 cut(s)	26, 441, 638, 720, 917
Msp20I	TGGCCA	2 cut(s)	755, 812
MspA1I	CMGCKG	2 cut(s)	527, 1085
MspI	CCGG	1 cut(s)	136
MspR9I	CCNGG	1 cut(s)	465
Mva1269I	GAATGC	1 cut(s)	800
MvaI	CCWGG	1 cut(s)	465
MwoI	GCNNNNNNNGC	4 cut(s)	145, 680, 1013, 1076
NaeI	GCCGGC	1 cut(s)	137
NdeI	CATATG	1 cut(s)	1025
NdeII	GATC	2 cut(s)	378, 820
NgoMIV	GCCGGC	1 cut(s)	135
NlaIII	CATG	6 cut(s)	69, 73, 102, 1057, 1192, 1228
NlaIV	GGNNCC	1 cut(s)	1007
NmeAIII	GCCGAG	1 cut(s)	475
NmuCI	GTSAC	2 cut(s)	363, 1158
NsiI	ATGCAT	2 cut(s)	71, 346
NspI	RCATGY	3 cut(s)	69, 73, 1192
PaeI	GCATGC	2 cut(s)	69, 73
PctI	GAATGC	1 cut(s)	800
PdiI	GCCGGC	1 cut(s)	137
PdmI	GAANNNNTTC	2 cut(s)	328, 422
PfeI	GAWTC	4 cut(s)	454, 563, 1032, 1088
PflMI	CCANNNNNTGG	1 cut(s)	1054
PkrI	GCNGC	5 cut(s)	75, 526, 1069, 1081, 1084
PshBI	ATTAAT	1 cut(s)	441
PsiI	TTATAA	1 cut(s)	251
Psp6I	CCWGG	1 cut(s)	463
PspFI	CCCAGC	1 cut(s)	557
PspGI	CCWGG	1 cut(s)	463
PspN4I	GGNNCC	1 cut(s)	1007
PspOMI	GGGCCC	1 cut(s)	1005
PspPI	GGNCC	2 cut(s)	1005, 1006
PstI	CTGCAG	3 cut(s)	685, 1072, 1084
PvuII	CAGCTG	2 cut(s)	527, 1085
RsaI	GTAC	1 cut(s)	696
RsaNI	GTAC	1 cut(s)	695
SaqAI	TTAA	5 cut(s)	26, 441, 638, 720, 917
SatI	GCNGC	5 cut(s)	74, 525, 1068, 1080, 1083
Sau3AI	GATC	2 cut(s)	378, 820
Sau96I	GGNCC	2 cut(s)	1005, 1006
ScrFI	CCNGG	1 cut(s)	465
SduI	GDGCHC	2 cut(s)	1009, 1118
SfaNI	GCATC	3 cut(s)	399, 899, 1238
SfcI	CTRYAG	4 cut(s)	60, 681, 1068, 1080
SphI	GCATGC	2 cut(s)	69, 73
Sse9I	AATT	9 cut(s)	9, 175, 244, 271, 418, 438, 580, 717, 1049
SsiI	CCGC	4 cut(s)	299, 488, 770, 937
SspMI	CTAG	4 cut(s)	279, 593, 947, 1185
StyD4I	CCNGG	1 cut(s)	463
StyI	CCWWGG	1 cut(s)	319
TaaI	ACNGT	5 cut(s)	84, 339, 400, 551, 1043
TaiI	ACGT	1 cut(s)	810
TaqI	TCGA	3 cut(s)	16, 123, 1243
TasI	AATT	9 cut(s)	9, 175, 244, 271, 418, 438, 580, 717, 1049
TatI	WGTACW	1 cut(s)	694
TfiI	GAWTC	4 cut(s)	454, 563, 1032, 1088
Tru1I	TTAA	5 cut(s)	26, 441, 638, 720, 917
Tru9I	TTAA	5 cut(s)	26, 441, 638, 720, 917
TscAI	CASTG	5 cut(s)	87, 372, 388, 685, 830
TseFI	GTSAC	2 cut(s)	363, 1158
TseI	GCWGC	5 cut(s)	73, 524, 1067, 1079, 1082
Tsp45I	GTSAC	2 cut(s)	363, 1158
TspDTI	ATGAA	2 cut(s)	954, 1042
TspRI	CASTG	5 cut(s)	87, 372, 388, 685, 830
Van91I	CCANNNNNTGG	1 cut(s)	1054
VspI	ATTAAT	1 cut(s)	441
XapI	RAATTY	1 cut(s)	9
XceI	RCATGY	3 cut(s)	69, 73, 1192
XmnI	GAANNNNTTC	2 cut(s)	328, 422
XspI	CTAG	4 cut(s)	279, 593, 947, 1185
Zsp2I	ATGCAT	2 cut(s)	71, 346

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.