Rw1G025310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006421
Physical Location & Seq
Forward (+)
51918169 .. 51918699
531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025310.1

Sequence Viewer

Length: 531 bp
ATGGTTCGAACCAAGGTTACCCCTCGCAGAGGTGTCACTCAACTTTGTGTTCTCTCACTGGAGGAAGAAGGTCGTCAAGCGGCCGCTAGAGCTGGCATCACTCGTCCTGGGGACCATCGTCCTATACGTGAGCGTACCCGCAGTCCCCCTCCTCTGCCTCGTCGCTCTCCCAGACTGCATCCCGGCGAGTCCTCTTCCTCACCAGCTGCTCGTGCTCCTCGGCCTGTGGCCACCCTGGAAAGCTTGTCGGATTCGATTGATGATTTGCGTTCCTCTCTCCGCGATGGTATTATGGTGACAGATTGGAGAGTCACATGCATGATCGATTACATCTCTGAGATGAACTTCTCTTTGATCCGATGTCACACTGCAATAAACAAGCTTGCTCAGGAAGTCAATAACTTGCAGAGTTATCCTCCTGGGTTTCCTCGCAAGGACGCTACTGCATCACACCATGAGGACCCTCCTCCGGAGGCTGAAACCAGCAAGAGGGCTGCCACTCACCCGCATACCGCTTCTCCAAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

19.51

Weight (kDa)

9.49

Isoelectric Point (pI)

69.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 282
AccIII TCCGGA 1 cut(s) 469
AciI CCGC 6 cut(s) 80, 84, 139, 280, 506, 513
AclWI GGATC 1 cut(s) 349
AcoI YGGCCR 2 cut(s) 81, 228
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 3 cut(s) 29, 469, 489
AjnI CCWGG 3 cut(s) 106, 234, 418
AluBI AGCT 4 cut(s) 92, 206, 243, 382
AluI AGCT 4 cut(s) 92, 206, 243, 382
Alw21I GWGCWC 1 cut(s) 217
AlwI GGATC 1 cut(s) 349
Aor13HI TCCGGA 1 cut(s) 469
AoxI GGCC 3 cut(s) 81, 221, 228
ApeKI GCWGC 2 cut(s) 206, 494
AspS9I GGNCC 2 cut(s) 112, 460
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 3 cut(s) 192, 307, 494
AsuII TTCGAA 1 cut(s) 7
AvaII GGWCC 2 cut(s) 112, 460
BalI TGGCCA 1 cut(s) 230
BauI CACGAG 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 2 cut(s) 193, 481
BccI CCATC 2 cut(s) 123, 278
BciT130I CCWGG 3 cut(s) 108, 236, 420
BcnI CCSGG 1 cut(s) 183
BfaI CTAG 1 cut(s) 87
BisI GCNGC 4 cut(s) 81, 84, 207, 495
BlsI GCNGC 4 cut(s) 82, 85, 208, 496
Bme1390I CCNGG 4 cut(s) 108, 183, 236, 420
Bme18I GGWCC 2 cut(s) 112, 460
BmgT120I GGNCC 2 cut(s) 112, 460
BmiI GGNNCC 2 cut(s) 113, 462
BmrFI CCNGG 4 cut(s) 108, 183, 236, 420
BmsI GCATC 3 cut(s) 105, 187, 455
BoxI GACNNNNGTC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 400, 432
BpmI CTGGAG 1 cut(s) 80
Bpu10I CCTNAGC 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 7
BpuMI CCSGG 1 cut(s) 183
Bsa29I ATCGAT 1 cut(s) 324
BsaAI YACGTR 1 cut(s) 128
BsaJI CCNNGG 5 cut(s) 12, 107, 218, 234, 419
BsaWI WCCGGW 1 cut(s) 469
BsaXI ACNNNNNCTCC 1 cut(s) 502
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 469, 489
Bse1I ACTGG 1 cut(s) 63
BseAI TCCGGA 1 cut(s) 469
BseBI CCWGG 3 cut(s) 108, 236, 420
BseCI ATCGAT 1 cut(s) 324
BseDI CCNNGG 5 cut(s) 12, 107, 218, 234, 419
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 3 cut(s) 29, 469, 489
BseMII CTCAG 2 cut(s) 327, 401
BseNI ACTGG 1 cut(s) 63
BseRI GAGGAG 3 cut(s) 141, 207, 456
BseX3I CGGCCG 1 cut(s) 81
BseXI GCAGC 2 cut(s) 193, 481
Bsh1236I CGCG 1 cut(s) 282
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 3 cut(s) 83, 223, 230
BshVI ATCGAT 1 cut(s) 324
BsiEI CGRYCG 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 2 cut(s) 183, 470
BslFI GGGAC 2 cut(s) 125, 129
BslI CCNNNNNNNGG 3 cut(s) 29, 469, 489
BsmFI GGGAC 2 cut(s) 125, 129
BsnI GGCC 3 cut(s) 83, 223, 230
Bsp119I TTCGAA 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 217
Bsp13I TCCGGA 1 cut(s) 469
Bsp143I GATC 2 cut(s) 321, 354
BspACI CCGC 6 cut(s) 80, 84, 139, 280, 506, 513
BspANI GGCC 3 cut(s) 83, 223, 230
BspCNI CTCAG 2 cut(s) 328, 400
BspDI ATCGAT 1 cut(s) 324
BspEI TCCGGA 1 cut(s) 469
BspFNI CGCG 1 cut(s) 282
BspLI GGNNCC 2 cut(s) 113, 462
BspPI GGATC 1 cut(s) 349
BspT104I TTCGAA 1 cut(s) 7
BsrI ACTGG 1 cut(s) 63
BssECI CCNNGG 5 cut(s) 12, 107, 218, 234, 419
BssMI GATC 2 cut(s) 321, 354
BssSI CACGAG 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 12
Bst2BI CACGAG 1 cut(s) 210
Bst2UI CCWGG 3 cut(s) 108, 236, 420
Bst6I CTCTTC 1 cut(s) 199
BstBAI YACGTR 1 cut(s) 128
BstBI TTCGAA 1 cut(s) 7
BstC8I GCNNGC 2 cut(s) 94, 384
BstDEI CTNAG 2 cut(s) 336, 387
BstEII GGTNACC 1 cut(s) 16
BstENI CCTNNNNNAGG 1 cut(s) 27
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 1 cut(s) 282
BstKTI GATC 2 cut(s) 324, 357
BstMBI GATC 2 cut(s) 321, 354
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 2 cut(s) 89, 212
BstNI CCWGG 3 cut(s) 108, 236, 420
BstNSI RCATGY 1 cut(s) 318
BstPAI GACNNNNGTC 1 cut(s) 117
BstPI GGTNACC 1 cut(s) 16
BstSCI CCNGG 4 cut(s) 106, 181, 234, 418
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 2 cut(s) 193, 481
BstZI CGGCCG 1 cut(s) 81
Bsu15I ATCGAT 1 cut(s) 324
BsuRI GGCC 3 cut(s) 83, 223, 230
BsuTUI ATCGAT 1 cut(s) 324
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 1 cut(s) 178
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 2 cut(s) 56, 366
Cac8I GCNNGC 2 cut(s) 94, 384
CciNI GCGGCCGC 1 cut(s) 81
Cfr13I GGNCC 2 cut(s) 112, 460
ClaI ATCGAT 1 cut(s) 324
CseI GACGC 1 cut(s) 446
Csp6I GTAC 1 cut(s) 135
CviAII CATG 3 cut(s) 315, 319, 455
CviJI RGCY 9 cut(s) 83, 92, 206, 223, 230, 243, 382, 476, 494
CviKI_1 RGCY 9 cut(s) 83, 92, 206, 223, 230, 243, 382, 476, 494
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 2 cut(s) 336, 387
DpnI GATC 2 cut(s) 323, 356
DpnII GATC 2 cut(s) 321, 354
EaeI YGGCCR 2 cut(s) 81, 228
EagI CGGCCG 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 199
EarI CTCTTC 1 cut(s) 199
EclXI CGGCCG 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 12
Eco47I GGWCC 2 cut(s) 112, 460
Eco52I CGGCCG 1 cut(s) 81
Eco91I GGTNACC 1 cut(s) 16
EcoNI CCTNNNNNAGG 1 cut(s) 27
EcoO109I RGGNCCY 1 cut(s) 460
EcoO65I GGTNACC 1 cut(s) 16
EcoRII CCWGG 3 cut(s) 106, 234, 418
EcoT14I CCWWGG 1 cut(s) 12
EcoT22I ATGCAT 1 cut(s) 320
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 3 cut(s) 318, 322, 458
FaiI YATR 6 cut(s) 125, 293, 316, 320, 456, 510
FaqI GGGAC 2 cut(s) 125, 129
FatI CATG 3 cut(s) 314, 318, 454
FauI CCCGC 2 cut(s) 146, 513
Fnu4HI GCNGC 4 cut(s) 81, 84, 207, 495
FokI GGATG 1 cut(s) 165
Fsp4HI GCNGC 4 cut(s) 81, 84, 207, 495
FspBI CTAG 1 cut(s) 87
GluI GCNGC 4 cut(s) 81, 84, 207, 495
GsuI CTGGAG 1 cut(s) 80
HaeIII GGCC 3 cut(s) 83, 223, 230
HapII CCGG 2 cut(s) 183, 470
HgaI GACGC 1 cut(s) 446
Hin1II CATG 3 cut(s) 318, 322, 458
HindIII AAGCTT 2 cut(s) 241, 380
HinfI GANTC 3 cut(s) 188, 251, 309
HpaII CCGG 2 cut(s) 183, 470
HphI GGTGA 3 cut(s) 192, 307, 494
Hpy188I TCNGA 3 cut(s) 250, 337, 359
Hpy188III TCNNGA 2 cut(s) 389, 470
Hpy99I CGWCG 1 cut(s) 165
HpyAV CCTTC 1 cut(s) 62
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 5 cut(s) 178, 318, 371, 406, 446
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 212
HpyF3I CTNAG 2 cut(s) 336, 387
HpySE526I ACGT 1 cut(s) 127
Hsp92II CATG 3 cut(s) 318, 322, 458
Kpn2I TCCGGA 1 cut(s) 469
Kzo9I GATC 2 cut(s) 321, 354
LmnI GCTCC 1 cut(s) 220
Lsp1109I GCAGC 2 cut(s) 193, 481
LweI GCATC 3 cut(s) 105, 187, 455
MaeI CTAG 1 cut(s) 87
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 5 cut(s) 16, 34, 295, 310, 362
MalI GATC 2 cut(s) 323, 356
MboI GATC 2 cut(s) 321, 354
MboII GAAGA 2 cut(s) 77, 186
MhlI GDGCHC 1 cut(s) 217
MlsI TGGCCA 1 cut(s) 230
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 2 cut(s) 197, 318
MmeI TCCRAC 1 cut(s) 228
Mox20I TGGCCA 1 cut(s) 230
Mph1103I ATGCAT 1 cut(s) 320
MroI TCCGGA 1 cut(s) 469
MscI TGGCCA 1 cut(s) 230
MslI CAYNNNNRTG 1 cut(s) 317
Msp20I TGGCCA 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 2 cut(s) 183, 470
MspR9I CCNGG 4 cut(s) 108, 183, 236, 420
MvaI CCWGG 3 cut(s) 108, 236, 420
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 2 cut(s) 89, 212
NciI CCSGG 1 cut(s) 183
NdeII GATC 2 cut(s) 321, 354
NlaIII CATG 3 cut(s) 318, 322, 458
NlaIV GGNNCC 2 cut(s) 113, 462
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 4 cut(s) 34, 295, 310, 362
NotI GCGGCCGC 1 cut(s) 81
NsiI ATGCAT 1 cut(s) 320
NspI RCATGY 1 cut(s) 318
NspV TTCGAA 1 cut(s) 7
PcsI WCGNNNNNNNCGW 1 cut(s) 124
PfeI GAWTC 1 cut(s) 251
PkrI GCNGC 4 cut(s) 82, 85, 208, 496
PleI GAGTC 2 cut(s) 196, 317
PpsI GAGTC 2 cut(s) 196, 317
Ppu21I YACGTR 1 cut(s) 128
PpuMI RGGWCCY 1 cut(s) 460
PshAI GACNNNNGTC 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 460
Psp6I CCWGG 3 cut(s) 106, 234, 418
PspEI GGTNACC 1 cut(s) 16
PspGI CCWGG 3 cut(s) 106, 234, 418
PspN4I GGNNCC 2 cut(s) 113, 462
PspPI GGNCC 2 cut(s) 112, 460
PspPPI RGGWCCY 1 cut(s) 460
PsrI GAACNNNNNNTAC 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
RseI CAYNNNNRTG 1 cut(s) 317
SatI GCNGC 4 cut(s) 81, 84, 207, 495
Sau3AI GATC 2 cut(s) 321, 354
Sau96I GGNCC 2 cut(s) 112, 460
SchI GAGTC 2 cut(s) 197, 318
ScrFI CCNGG 4 cut(s) 108, 183, 236, 420
SduI GDGCHC 1 cut(s) 217
SetI ASST 8 cut(s) 18, 34, 73, 94, 130, 208, 245, 384
SfaNI GCATC 3 cut(s) 105, 187, 455
SfuI TTCGAA 1 cut(s) 7
SinI GGWCC 2 cut(s) 112, 460
SmiMI CAYNNNNRTG 1 cut(s) 317
SsiI CCGC 6 cut(s) 80, 84, 139, 280, 506, 513
SspMI CTAG 1 cut(s) 87
StyD4I CCNGG 4 cut(s) 106, 181, 234, 418
StyI CCWWGG 1 cut(s) 12
TaiI ACGT 1 cut(s) 130
TaqI TCGA 3 cut(s) 7, 254, 324
TauI GCSGC 2 cut(s) 83, 86
TfiI GAWTC 1 cut(s) 251
TscAI CASTG 2 cut(s) 63, 373
TseFI GTSAC 4 cut(s) 34, 295, 310, 362
TseI GCWGC 2 cut(s) 206, 494
Tsp45I GTSAC 4 cut(s) 34, 295, 310, 362
TspDTI ATGAA 1 cut(s) 356
TspRI CASTG 2 cut(s) 63, 373
VpaK11BI GGWCC 2 cut(s) 112, 460
XagI CCTNNNNNAGG 1 cut(s) 27
XceI RCATGY 1 cut(s) 318
XspI CTAG 1 cut(s) 87
Zsp2I ATGCAT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.