Rw3G020540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0006421
Physical Location & Seq
Forward (+)
24053689 .. 24054219
531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G020540.1

Sequence Viewer

Length: 531 bp
ATGGCTCGAACCAAGGTAACCCCTCGCAGGGGCGTCAATCAACGCCGTGTTCTCTCTTTGGAAGAAGAAGGTCGTCAAGCGGCCACTAGAGCTGGGCTTACTCGTCCATGGGACCATCGTCCTATCCGTGAGCGTACCCGCAGTCCCCCTCCTCTGCCTCGTCGCTCTCCCAGACTGCATCCCAGAGAGTCTTCTTCCTCACCAGCTGCTCGTGCTCCTCGGCCTATGGCCACCCTGGAAAGCTTGTCGGATTTGATTGATGATTTGCGTTCCTCTCTCCGCGATGGTATTATGGTGACAGATTGGAGAGTCAATTGCATGATCGATTACATCTCTGAGATGAACTGCTCTTTGATCCGCTGTCACACTGCAATAAACAAGCTTGCTCAGGAAGTCAATAACTTGCAGAGTTATCCTCCTGGGTTTCCTCGCAAGGACGCTACTGCATCACACCATGAGAACCCTCCTCAGGAGGCTGAAACCAGCAAGAAGGCTTCCACTCGCCCGCACACCGCTCCTCCAAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

19.92

Weight (kDa)

10.36

Isoelectric Point (pI)

71.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 515
AccII CGCG 1 cut(s) 282
AciI CCGC 6 cut(s) 80, 139, 280, 358, 506, 513
AclWI GGATC 1 cut(s) 349
AcoI YGGCCR 2 cut(s) 81, 228
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 4 cut(s) 27, 28, 29, 469
AjnI CCWGG 2 cut(s) 234, 418
AluBI AGCT 4 cut(s) 92, 206, 243, 382
AluI AGCT 4 cut(s) 92, 206, 243, 382
Alw21I GWGCWC 1 cut(s) 217
AlwI GGATC 1 cut(s) 349
AoxI GGCC 3 cut(s) 81, 221, 228
ApeKI GCWGC 1 cut(s) 206
AspS9I GGNCC 1 cut(s) 112
AsuHPI GGTGA 2 cut(s) 192, 307
AvaII GGWCC 1 cut(s) 112
AxyI CCTNAGG 1 cut(s) 468
BalI TGGCCA 1 cut(s) 230
BauI CACGAG 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 1 cut(s) 193
BccI CCATC 2 cut(s) 123, 278
BceAI ACGGC 1 cut(s) 30
BciT130I CCWGG 2 cut(s) 236, 420
BfaI CTAG 1 cut(s) 87
BisI GCNGC 2 cut(s) 81, 207
BlsI GCNGC 2 cut(s) 82, 208
Bme1390I CCNGG 2 cut(s) 236, 420
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 1 cut(s) 113
BmrFI CCNGG 2 cut(s) 236, 420
BmsI GCATC 2 cut(s) 187, 455
BoxI GACNNNNGTC 1 cut(s) 117
BpiI GAAGAC 1 cut(s) 183
BplI GAGNNNNNCTC 2 cut(s) 400, 432
Bpu10I CCTNAGC 1 cut(s) 387
Bsa29I ATCGAT 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 5 cut(s) 12, 107, 218, 234, 419
BsaXI ACNNNNNCTCC 1 cut(s) 502
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 28, 29, 469
Bse21I CCTNAGG 1 cut(s) 468
BseBI CCWGG 2 cut(s) 236, 420
BseCI ATCGAT 1 cut(s) 324
BseDI CCNNGG 5 cut(s) 12, 107, 218, 234, 419
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 4 cut(s) 27, 28, 29, 469
BseMII CTCAG 3 cut(s) 327, 401, 482
BseRI GAGGAG 4 cut(s) 141, 207, 456, 507
BseXI GCAGC 1 cut(s) 193
BseYI CCCAGC 1 cut(s) 92
Bsh1236I CGCG 1 cut(s) 282
BshFI GGCC 3 cut(s) 83, 223, 230
BshVI ATCGAT 1 cut(s) 324
BsiHKAI GWGCWC 1 cut(s) 217
BslFI GGGAC 2 cut(s) 125, 129
BslI CCNNNNNNNGG 4 cut(s) 27, 28, 29, 469
BsmFI GGGAC 2 cut(s) 125, 129
BsnI GGCC 3 cut(s) 83, 223, 230
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 2 cut(s) 321, 354
Bsp19I CCATGG 1 cut(s) 107
BspACI CCGC 6 cut(s) 80, 139, 280, 358, 506, 513
BspANI GGCC 3 cut(s) 83, 223, 230
BspCNI CTCAG 3 cut(s) 328, 400, 481
BspDI ATCGAT 1 cut(s) 324
BspFNI CGCG 1 cut(s) 282
BspLI GGNNCC 1 cut(s) 113
BspPI GGATC 1 cut(s) 349
BsrBI CCGCTC 1 cut(s) 515
BssECI CCNNGG 5 cut(s) 12, 107, 218, 234, 419
BssMI GATC 2 cut(s) 321, 354
BssNI GRCGYC 1 cut(s) 33
BssSI CACGAG 1 cut(s) 210
BssT1I CCWWGG 2 cut(s) 12, 107
Bst2BI CACGAG 1 cut(s) 210
Bst2UI CCWGG 2 cut(s) 236, 420
BstACI GRCGYC 1 cut(s) 33
BstC8I GCNNGC 2 cut(s) 384, 506
BstDEI CTNAG 3 cut(s) 336, 387, 468
BstDSI CCRYGG 1 cut(s) 107
BstEII GGTNACC 1 cut(s) 16
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 1 cut(s) 282
BstKTI GATC 2 cut(s) 324, 357
BstMBI GATC 2 cut(s) 321, 354
BstMWI GCNNNNNNNGC 2 cut(s) 89, 212
BstNI CCWGG 2 cut(s) 236, 420
BstPAI GACNNNNGTC 1 cut(s) 117
BstPI GGTNACC 1 cut(s) 16
BstSCI CCNGG 2 cut(s) 234, 418
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 193
BstV2I GAAGAC 1 cut(s) 183
Bsu15I ATCGAT 1 cut(s) 324
Bsu36I CCTNAGG 1 cut(s) 468
BsuRI GGCC 3 cut(s) 83, 223, 230
BsuTUI ATCGAT 1 cut(s) 324
BtgI CCRYGG 1 cut(s) 107
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 1 cut(s) 178
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 2 cut(s) 384, 506
Cfr13I GGNCC 1 cut(s) 112
ClaI ATCGAT 1 cut(s) 324
CseI GACGC 2 cut(s) 22, 446
Csp6I GTAC 1 cut(s) 135
CviAII CATG 3 cut(s) 108, 319, 455
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 3 cut(s) 336, 387, 468
DpnI GATC 2 cut(s) 323, 356
DpnII GATC 2 cut(s) 321, 354
EaeI YGGCCR 2 cut(s) 81, 228
Eco130I CCWWGG 2 cut(s) 12, 107
Eco47I GGWCC 1 cut(s) 112
Eco81I CCTNAGG 1 cut(s) 468
Eco91I GGTNACC 1 cut(s) 16
EcoO65I GGTNACC 1 cut(s) 16
EcoRII CCWGG 2 cut(s) 234, 418
EcoT14I CCWWGG 2 cut(s) 12, 107
ErhI CCWWGG 2 cut(s) 12, 107
FaeI CATG 3 cut(s) 111, 322, 458
FaiI YATR 5 cut(s) 109, 227, 293, 320, 456
FaqI GGGAC 2 cut(s) 125, 129
FatI CATG 3 cut(s) 107, 318, 454
FauI CCCGC 2 cut(s) 146, 513
Fnu4HI GCNGC 2 cut(s) 81, 207
FokI GGATG 1 cut(s) 165
Fsp4HI GCNGC 2 cut(s) 81, 207
FspBI CTAG 1 cut(s) 87
GluI GCNGC 2 cut(s) 81, 207
GsaI CCCAGC 1 cut(s) 96
HaeIII GGCC 3 cut(s) 83, 223, 230
HgaI GACGC 2 cut(s) 22, 446
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 3 cut(s) 111, 322, 458
HindIII AAGCTT 2 cut(s) 241, 380
HinfI GANTC 2 cut(s) 188, 309
HphI GGTGA 2 cut(s) 192, 307
Hpy188I TCNGA 2 cut(s) 250, 337
Hpy188III TCNNGA 2 cut(s) 389, 470
Hpy99I CGWCG 1 cut(s) 165
HpyAV CCTTC 2 cut(s) 62, 484
HpyCH4V TGCA 5 cut(s) 178, 318, 371, 406, 446
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 212
HpyF3I CTNAG 3 cut(s) 336, 387, 468
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 3 cut(s) 111, 322, 458
Kzo9I GATC 2 cut(s) 321, 354
LmnI GCTCC 2 cut(s) 220, 520
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 2 cut(s) 187, 455
MaeI CTAG 1 cut(s) 87
MaeIII GTNAC 3 cut(s) 16, 295, 362
MalI GATC 2 cut(s) 323, 356
MbiI CCGCTC 1 cut(s) 515
MboI GATC 2 cut(s) 321, 354
MboII GAAGA 4 cut(s) 74, 77, 183, 186
MfeI CAATTG 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 217
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 1 cut(s) 313
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 2 cut(s) 197, 318
MmeI TCCRAC 1 cut(s) 228
Mox20I TGGCCA 1 cut(s) 230
MscI TGGCCA 1 cut(s) 230
Msp20I TGGCCA 1 cut(s) 230
MspA1I CMGCKG 2 cut(s) 206, 360
MspR9I CCNGG 2 cut(s) 236, 420
MunI CAATTG 1 cut(s) 313
MvaI CCWGG 2 cut(s) 236, 420
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 2 cut(s) 89, 212
NcoI CCATGG 1 cut(s) 107
NdeII GATC 2 cut(s) 321, 354
NlaIII CATG 3 cut(s) 111, 322, 458
NlaIV GGNNCC 1 cut(s) 113
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 2 cut(s) 295, 362
PcsI WCGNNNNNNNCGW 1 cut(s) 124
PkrI GCNGC 2 cut(s) 82, 208
PleI GAGTC 2 cut(s) 196, 317
PpsI GAGTC 2 cut(s) 196, 317
PshAI GACNNNNGTC 1 cut(s) 117
Psp6I CCWGG 2 cut(s) 234, 418
PspEI GGTNACC 1 cut(s) 16
PspFI CCCAGC 1 cut(s) 92
PspGI CCWGG 2 cut(s) 234, 418
PspN4I GGNNCC 1 cut(s) 113
PspPI GGNCC 1 cut(s) 112
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
SatI GCNGC 2 cut(s) 81, 207
Sau3AI GATC 2 cut(s) 321, 354
Sau96I GGNCC 1 cut(s) 112
SchI GAGTC 2 cut(s) 197, 318
ScrFI CCNGG 2 cut(s) 236, 420
SduI GDGCHC 1 cut(s) 217
SetI ASST 6 cut(s) 18, 73, 94, 208, 245, 384
SfaNI GCATC 2 cut(s) 187, 455
SinI GGWCC 1 cut(s) 112
Sse9I AATT 1 cut(s) 313
SsiI CCGC 6 cut(s) 80, 139, 280, 358, 506, 513
SspMI CTAG 1 cut(s) 87
StyD4I CCNGG 2 cut(s) 234, 418
StyI CCWWGG 2 cut(s) 12, 107
TaqI TCGA 2 cut(s) 7, 324
TasI AATT 1 cut(s) 313
TauI GCSGC 1 cut(s) 83
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 2 cut(s) 295, 362
TseI GCWGC 1 cut(s) 206
Tsp45I GTSAC 2 cut(s) 295, 362
TspDTI ATGAA 1 cut(s) 356
TspGWI ACGGA 1 cut(s) 116
TspRI CASTG 1 cut(s) 373
VpaK11BI GGWCC 1 cut(s) 112
XspI CTAG 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.