Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0007111

Physical Location & Seq

Genomic Coordinates Reverse (-)

16265874 .. 16268880

Gene Structure 3007 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G014440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1005 bp

ATGGACGAGATGGCCGCCTATTACCCTCCACCGCCTCCGCCACCAGGCTCCGTCCACTATCCATACTACCACCACCAGCCACCTCAACCGCCTGCCCCCGTGGCCCCTCCGCCGCCGCCGCAGCACCACCACCACCACTTGGTTCATCAGCCCTACTACCCTCCCCAGCCTCAGCCTTACGCTCCCTACGCGCCGCCACTCGTCGCCCAGGCCGCCTACGACGAGGTCCGGACCCTCTTCGTGGCCGGCCTACCCGAAGATGTCAAGCCCCGTGAAATCTACAACCTCTTCCGCGAGTTTCCCGGTTACGAGTCCTCTCACCTCCGAACCCCCACCGAGAAAACTCAGCCTTTTGCGTTTGCGGTGTTCTTGGACCAGCAATCTGCGATCGGAGCAATGCAAGCACTGAATGGGATGGTATTTGATCTTGAGAAGGGGTCGACATTATATATAGATCTAGCAAAATCTAATTCTAGGTCCAAGCGCTCAAGAACAGACGATGAAAGATCTGGCTCAGATAAGAAAGCTAGAGGTTCATCATATTCAAGGGCCACTTCTGATCCTGCAGGTGTTGGCAGCATTCACATGCCTGGAATGGGTAATTCTGCTTACAACATGATTGGTTATCCATCTGCACCAAGTCATGGCAGCTTCAGTGGCAGTGGTGTAAATGAGCCAATAGCCTCGAACATGAATAGTTCTTCAGCACCACATTTTCCACAAAACAATACTCCATGCCCAACACTTTTTGTGGCTAATTTGGGGCCAACTTGTACGGAGCAAGAGCTAGTTCAAGTATTTTCGAGGTGCCCTGGGTTTATAAAATTGAAGATGCAGAGCACATATGGGGCTCCAGTTGCATTTGTTGATTTTCAGGATACTGCTAGCTCAACTGGCGCACTGGGTCATCTGCAGGGCACAATTCTCTACTCGTCAACATCTGGAGAGGGCATGAGATTAGAGTTTGCCAAATCACGAATGGGAATGCGGAAGAAGCCGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0007275 GO:0008150 GO:0008284 GO:0009653 GO:0009790 GO:0009887 GO:0009889 GO:0009966 GO:0009968 GO:0010468 GO:0010556 GO:0010646 GO:0010648 GO:0019219 GO:0019222 GO:0023051 GO:0023057 GO:0030510 GO:0030514 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0035239 GO:0035295 GO:0042127 GO:0042802 GO:0042803 GO:0044424 GO:0044464 GO:0045595 GO:0045596 GO:0046983 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048546 GO:0048557 GO:0048562 GO:0048565 GO:0048566 GO:0048568 GO:0048583 GO:0048585 GO:0048598 GO:0048660 GO:0048661 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051147 GO:0051148 GO:0051150 GO:0051151 GO:0051171 GO:0051252 GO:0055123 GO:0060255 GO:0065007 GO:0080090 GO:0090092 GO:0090101 GO:0090287 GO:0090288 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RRM_1

Protein Analysis

334

Amino Acids

36.42

Weight (kDa)

7.79

Isoelectric Point (pI)

59.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_1	PF00076	79 - 142	1.3e-11	RNA recognition motif
RRM_1	PF00076	249 - 312	2e-09	RNA recognition motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007111)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42240 AT2G42240 AT2G42240
fragaria_vesca	FvH4_1g16380 FvH4_1g16380
malus_domestica	MD02G1176300.v1.1 MD15G1286700.v1.1
prunus_persica	Prupe.6G231400_v2.0.a1 Prupe.6G231400_v2.0.a1 Prupe.6G231400_v2.0.a1 Prupe.6G231400_v2.0.a1 Prupe.6G231400_v2.0.a1
pyrus_communis	pycom02g14250 pycom15g25000
rosa_chinensis	RchiOBHm_Chr2g0106251
rosa_laevigata	RLG00000017483
rosa_multiflora	Rmu_sc0008603.1_g000013
rosa_roxburghii	Rroxscaffold_2G00137330
rosa_rugosa	Rorug02G0133700 Rorug02G0133800
rosa_samantha	Rh2AG184900 Rh2BG195200 Rh2CG190000 Rh2DG191100
rosa_wichuraiana	Rw2G014440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	821
AarI	CACCTGC	1 cut(s)	557
Acc36I	ACCTGC	1 cut(s)	557
AccB1I	GGYRCC	1 cut(s)	807
AccB7I	CCANNNNNTGG	2 cut(s)	139, 644
AccI	GTMKAC	1 cut(s)	440
AccII	CGCG	2 cut(s)	191, 294
AccIII	TCCGGA	1 cut(s)	228
AclWI	GGATC	1 cut(s)	554
AcoI	YGGCCR	2 cut(s)	12, 243
AcuI	CTGAAG	2 cut(s)	637, 687
AfaI	GTAC	1 cut(s)	775
AfeI	AGCGCT	1 cut(s)	485
AfiI	CCNNNNNNNGG	5 cut(s)	44, 139, 241, 596, 644
AgsI	TTSAA	3 cut(s)	546, 794, 829
AjnI	CCWGG	4 cut(s)	43, 207, 589, 811
AluBI	AGCT	4 cut(s)	527, 651, 787, 888
AluI	AGCT	4 cut(s)	527, 651, 787, 888
Alw21I	GWGCWC	1 cut(s)	842
AlwI	GGATC	1 cut(s)	554
Aor13HI	TCCGGA	1 cut(s)	228
Aor51HI	AGCGCT	1 cut(s)	485
AoxI	GGCC	7 cut(s)	12, 102, 210, 243, 247, 549, 764
ApeKI	GCWGC	3 cut(s)	121, 576, 648
AspLEI	GCGC	3 cut(s)	193, 486, 899
AspS9I	GGNCC	7 cut(s)	103, 226, 231, 373, 477, 549, 764
AsuC2I	CCSGG	1 cut(s)	303
AsuHPI	GGTGA	1 cut(s)	311
AsuNHI	GCTAGC	1 cut(s)	884
AvaII	GGWCC	4 cut(s)	226, 231, 373, 477
BaeGI	GKGCMC	2 cut(s)	812, 920
BanI	GGYRCC	1 cut(s)	807
BanII	GRGCYC	1 cut(s)	853
Bbv12I	GWGCWC	1 cut(s)	842
BbvCI	CCTCAGC	1 cut(s)	171
BbvI	GCAGC	3 cut(s)	133, 588, 660
BccI	CCATC	3 cut(s)	4, 409, 637
BciT130I	CCWGG	4 cut(s)	45, 209, 591, 813
BciVI	GTATCC	1 cut(s)	871
BcnI	CCSGG	1 cut(s)	303
BfaI	CTAG	5 cut(s)	458, 474, 528, 788, 885
BfmI	CTRYAG	2 cut(s)	564, 911
BfoI	RGCGCY	1 cut(s)	487
BfuAI	ACCTGC	1 cut(s)	557
BfuI	GTATCC	1 cut(s)	871
BglI	GCCNNNNNGGC	1 cut(s)	101
BglII	AGATCT	2 cut(s)	454, 506
BisI	GCNGC	9 cut(s)	15, 113, 116, 119, 122, 194, 213, 577, 649
BlsI	GCNGC	9 cut(s)	16, 114, 117, 120, 123, 195, 214, 578, 650
Bme1390I	CCNGG	5 cut(s)	45, 209, 303, 591, 813
Bme18I	GGWCC	4 cut(s)	226, 231, 373, 477
BmgT120I	GGNCC	7 cut(s)	103, 226, 231, 373, 477, 549, 764
BmiI	GGNNCC	6 cut(s)	49, 105, 233, 765, 809, 852
BmrFI	CCNGG	5 cut(s)	45, 209, 303, 591, 813
BmrI	ACTGGG	1 cut(s)	911
BmsI	GCATC	1 cut(s)	822
BmtI	GCTAGC	1 cut(s)	888
BmuI	ACTGGG	1 cut(s)	911
BpmI	CTGGAG	2 cut(s)	837, 963
Bpu10I	CCTNAGC	1 cut(s)	171
BpuEI	CTTGAG	2 cut(s)	449, 472
BpuMI	CCSGG	1 cut(s)	303
BsaJI	CCNNGG	4 cut(s)	99, 207, 811, 812
BsaWI	WCCGGW	1 cut(s)	228
Bsc4I	CCNNNNNNNGG	5 cut(s)	44, 139, 241, 596, 644
Bse118I	RCCGGY	1 cut(s)	245
Bse1I	ACTGG	3 cut(s)	854, 898, 906
Bse3DI	GCAATG	1 cut(s)	402
BseAI	TCCGGA	1 cut(s)	228
BseBI	CCWGG	4 cut(s)	45, 209, 591, 813
BseDI	CCNNGG	4 cut(s)	99, 207, 811, 812
BseGI	GGATG	1 cut(s)	420
BseLI	CCNNNNNNNGG	5 cut(s)	44, 139, 241, 596, 644
BseMI	GCAATG	1 cut(s)	402
BseMII	CTCAG	3 cut(s)	185, 359, 528
BseNI	ACTGG	3 cut(s)	854, 898, 906
BseSI	GKGCMC	2 cut(s)	812, 920
BseXI	GCAGC	3 cut(s)	133, 588, 660
BseYI	CCCAGC	1 cut(s)	165
BsgI	GTGCAG	1 cut(s)	618
Bsh1236I	CGCG	2 cut(s)	191, 294
Bsh1285I	CGRYCG	1 cut(s)	390
BshFI	GGCC	7 cut(s)	14, 104, 212, 245, 249, 551, 766
BshNI	GGYRCC	1 cut(s)	807
BsiEI	CGRYCG	1 cut(s)	390
BsiHKAI	GWGCWC	1 cut(s)	842
BsiSI	CCGG	3 cut(s)	229, 246, 303
BslI	CCNNNNNNNGG	5 cut(s)	44, 139, 241, 596, 644
BsmI	GAATGC	2 cut(s)	579, 990
BsnI	GGCC	7 cut(s)	14, 104, 212, 245, 249, 551, 766
Bsp1286I	GDGCHC	4 cut(s)	812, 842, 853, 920
Bsp13I	TCCGGA	1 cut(s)	228
Bsp143I	GATC	5 cut(s)	387, 424, 454, 506, 559
BspANI	GGCC	7 cut(s)	14, 104, 212, 245, 249, 551, 766
BspCNI	CTCAG	3 cut(s)	184, 358, 527
BspEI	TCCGGA	1 cut(s)	228
BspFNI	CGCG	2 cut(s)	191, 294
BspLI	GGNNCC	6 cut(s)	49, 105, 233, 765, 809, 852
BspMAI	CTGCAG	2 cut(s)	568, 915
BspMI	ACCTGC	1 cut(s)	557
BspOI	GCTAGC	1 cut(s)	888
BspPI	GGATC	1 cut(s)	554
BspT107I	GGYRCC	1 cut(s)	807
BsrDI	GCAATG	1 cut(s)	402
BsrFI	RCCGGY	1 cut(s)	245
BsrI	ACTGG	3 cut(s)	854, 898, 906
BssAI	RCCGGY	1 cut(s)	245
BssECI	CCNNGG	4 cut(s)	99, 207, 811, 812
BssMI	GATC	5 cut(s)	387, 424, 454, 506, 559
Bst2UI	CCWGG	4 cut(s)	45, 209, 591, 813
Bst6I	CTCTTC	2 cut(s)	242, 293
BstC8I	GCNNGC	4 cut(s)	93, 247, 402, 886
BstDEI	CTNAG	3 cut(s)	171, 345, 514
BstDSI	CCRYGG	1 cut(s)	99
BstF5I	GGATG	1 cut(s)	420
BstFNI	CGCG	2 cut(s)	191, 294
BstH2I	RGCGCY	1 cut(s)	487
BstHHI	GCGC	3 cut(s)	193, 486, 899
BstKTI	GATC	5 cut(s)	390, 427, 457, 509, 562
BstMBI	GATC	5 cut(s)	387, 424, 454, 506, 559
BstMCI	CGRYCG	1 cut(s)	390
BstNI	CCWGG	4 cut(s)	45, 209, 591, 813
BstNSI	RCATGY	1 cut(s)	589
BstSCI	CCNGG	5 cut(s)	43, 207, 301, 589, 811
BstSFI	CTRYAG	2 cut(s)	564, 911
BstSLI	GKGCMC	2 cut(s)	812, 920
BstUI	CGCG	2 cut(s)	191, 294
BstV1I	GCAGC	3 cut(s)	133, 588, 660
BstX2I	RGATCY	2 cut(s)	454, 506
BstYI	RGATCY	2 cut(s)	454, 506
BsuI	GTATCC	1 cut(s)	871
BsuRI	GGCC	7 cut(s)	14, 104, 212, 245, 249, 551, 766
BtgI	CCRYGG	1 cut(s)	99
BtsCI	GGATG	1 cut(s)	420
BtsI	GCAGTG	1 cut(s)	667
BtsIMutI	CAGTG	4 cut(s)	404, 661, 667, 899
BveI	ACCTGC	1 cut(s)	557
Cac8I	GCNNGC	4 cut(s)	93, 247, 402, 886
CfoI	GCGC	3 cut(s)	193, 486, 899
Cfr10I	RCCGGY	1 cut(s)	245
Cfr13I	GGNCC	7 cut(s)	103, 226, 231, 373, 477, 549, 764
Csp6I	GTAC	1 cut(s)	774
CviAII	CATG	6 cut(s)	586, 616, 644, 691, 735, 952
CviQI	GTAC	1 cut(s)	774
DdeI	CTNAG	3 cut(s)	171, 345, 514
DpnI	GATC	5 cut(s)	389, 426, 456, 508, 561
DpnII	GATC	5 cut(s)	387, 424, 454, 506, 559
EaeI	YGGCCR	2 cut(s)	12, 243
Eam1104I	CTCTTC	2 cut(s)	242, 293
EarI	CTCTTC	2 cut(s)	242, 293
EciI	GGCGGA	2 cut(s)	27, 99
Eco24I	GRGCYC	1 cut(s)	853
Eco47I	GGWCC	4 cut(s)	226, 231, 373, 477
Eco47III	AGCGCT	1 cut(s)	485
Eco57I	CTGAAG	2 cut(s)	637, 687
EcoRII	CCWGG	4 cut(s)	43, 207, 589, 811
EcoT38I	GRGCYC	1 cut(s)	853
FaeI	CATG	6 cut(s)	589, 619, 647, 694, 738, 955
FalI	AAGNNNNNCTT	2 cut(s)	538, 570
FatI	CATG	6 cut(s)	585, 615, 643, 690, 734, 951
FauNDI	CATATG	1 cut(s)	844
FblI	GTMKAC	1 cut(s)	440
Fnu4HI	GCNGC	9 cut(s)	15, 113, 116, 119, 122, 194, 213, 577, 649
FokI	GGATG	1 cut(s)	427
FriOI	GRGCYC	1 cut(s)	853
FseI	GGCCGGCC	1 cut(s)	249
Fsp4HI	GCNGC	9 cut(s)	15, 113, 116, 119, 122, 194, 213, 577, 649
FspBI	CTAG	5 cut(s)	458, 474, 528, 788, 885
GlaI	GCGC	3 cut(s)	192, 485, 898
GluI	GCNGC	9 cut(s)	15, 113, 116, 119, 122, 194, 213, 577, 649
GsaI	CCCAGC	1 cut(s)	169
GsuI	CTGGAG	2 cut(s)	837, 963
HaeII	RGCGCY	1 cut(s)	487
HaeIII	GGCC	7 cut(s)	14, 104, 212, 245, 249, 551, 766
HapII	CCGG	3 cut(s)	229, 246, 303
HhaI	GCGC	3 cut(s)	193, 486, 899
Hin1II	CATG	6 cut(s)	589, 619, 647, 694, 738, 955
Hin6I	GCGC	3 cut(s)	191, 484, 897
HinP1I	GCGC	3 cut(s)	191, 484, 897
HincII	GTYRAC	2 cut(s)	441, 936
HindII	GTYRAC	2 cut(s)	441, 936
HinfI	GANTC	1 cut(s)	311
HpaII	CCGG	3 cut(s)	229, 246, 303
HphI	GGTGA	1 cut(s)	311
Hpy166II	GTNNAC	3 cut(s)	55, 441, 936
Hpy188I	TCNGA	4 cut(s)	326, 392, 517, 559
Hpy188III	TCNNGA	6 cut(s)	229, 428, 489, 875, 942, 975
Hpy8I	GTNNAC	3 cut(s)	55, 441, 936
Hpy99I	CGWCG	2 cut(s)	206, 224
HpyAV	CCTTC	1 cut(s)	427
HpyCH4V	TGCA	6 cut(s)	400, 566, 635, 835, 860, 913
HpyF3I	CTNAG	3 cut(s)	171, 345, 514
Hsp92II	CATG	6 cut(s)	589, 619, 647, 694, 738, 955
HspAI	GCGC	3 cut(s)	191, 484, 897
Kpn2I	TCCGGA	1 cut(s)	228
KroI	GCCGGC	1 cut(s)	245
KroNI	GCCGGC	1 cut(s)	247
Kzo9I	GATC	5 cut(s)	387, 424, 454, 506, 559
LmnI	GCTCC	5 cut(s)	53, 187, 392, 778, 856
Lsp1109I	GCAGC	3 cut(s)	133, 588, 660
LweI	GCATC	1 cut(s)	822
MaeI	CTAG	5 cut(s)	458, 474, 528, 788, 885
MaeIII	GTNAC	1 cut(s)	305
MalI	GATC	5 cut(s)	389, 426, 456, 508, 561
MboI	GATC	5 cut(s)	387, 424, 454, 506, 559
MboII	GAAGA	6 cut(s)	229, 269, 280, 693, 841, 1003
MflI	RGATCY	2 cut(s)	454, 506
MhlI	GDGCHC	4 cut(s)	812, 842, 853, 920
MluCI	AATT	5 cut(s)	469, 601, 757, 824, 921
MlyI	GAGTC	1 cut(s)	320
MroI	TCCGGA	1 cut(s)	228
MroNI	GCCGGC	1 cut(s)	245
MslI	CAYNNNNRTG	1 cut(s)	584
MspI	CCGG	3 cut(s)	229, 246, 303
MspR9I	CCNGG	5 cut(s)	45, 209, 303, 591, 813
Mva1269I	GAATGC	2 cut(s)	579, 990
MvaI	CCWGG	4 cut(s)	45, 209, 591, 813
MvnI	CGCG	2 cut(s)	191, 294
NaeI	GCCGGC	1 cut(s)	247
NciI	CCSGG	1 cut(s)	303
NdeI	CATATG	1 cut(s)	844
NdeII	GATC	5 cut(s)	387, 424, 454, 506, 559
NgoMIV	GCCGGC	1 cut(s)	245
NheI	GCTAGC	1 cut(s)	884
NlaIII	CATG	6 cut(s)	589, 619, 647, 694, 738, 955
NlaIV	GGNNCC	6 cut(s)	49, 105, 233, 765, 809, 852
NspI	RCATGY	1 cut(s)	589
PaqCI	CACCTGC	1 cut(s)	557
PasI	CCCWGGG	1 cut(s)	812
PctI	GAATGC	2 cut(s)	579, 990
PdiI	GCCGGC	1 cut(s)	247
PflFI	GACNNNGTC	1 cut(s)	224
PflMI	CCANNNNNTGG	2 cut(s)	139, 644
PkrI	GCNGC	9 cut(s)	16, 114, 117, 120, 123, 195, 214, 578, 650
Ple19I	CGATCG	1 cut(s)	390
PleI	GAGTC	1 cut(s)	319
PpsI	GAGTC	1 cut(s)	319
PsiI	TTATAA	1 cut(s)	821
Psp6I	CCWGG	4 cut(s)	43, 207, 589, 811
PspFI	CCCAGC	1 cut(s)	165
PspGI	CCWGG	4 cut(s)	43, 207, 589, 811
PspN4I	GGNNCC	6 cut(s)	49, 105, 233, 765, 809, 852
PspPI	GGNCC	7 cut(s)	103, 226, 231, 373, 477, 549, 764
PstI	CTGCAG	2 cut(s)	568, 915
PsuI	RGATCY	2 cut(s)	454, 506
PsyI	GACNNNGTC	1 cut(s)	224
PvuI	CGATCG	1 cut(s)	390
RigI	GGCCGGCC	1 cut(s)	249
RsaI	GTAC	1 cut(s)	775
RsaNI	GTAC	1 cut(s)	774
RseI	CAYNNNNRTG	1 cut(s)	584
SalI	GTCGAC	1 cut(s)	439
SatI	GCNGC	9 cut(s)	15, 113, 116, 119, 122, 194, 213, 577, 649
Sau3AI	GATC	5 cut(s)	387, 424, 454, 506, 559
Sau96I	GGNCC	7 cut(s)	103, 226, 231, 373, 477, 549, 764
SbfI	CCTGCAGG	1 cut(s)	568
SchI	GAGTC	1 cut(s)	320
ScrFI	CCNGG	5 cut(s)	45, 209, 303, 591, 813
SdaI	CCTGCAGG	1 cut(s)	568
SduI	GDGCHC	4 cut(s)	812, 842, 853, 920
SfaNI	GCATC	1 cut(s)	822
SfcI	CTRYAG	2 cut(s)	564, 911
SinI	GGWCC	4 cut(s)	226, 231, 373, 477
SmiMI	CAYNNNNRTG	1 cut(s)	584
SmlI	CTYRAG	2 cut(s)	428, 487
SmoI	CTYRAG	2 cut(s)	428, 487
Sse8387I	CCTGCAGG	1 cut(s)	568
Sse9I	AATT	5 cut(s)	469, 601, 757, 824, 921
SspMI	CTAG	5 cut(s)	458, 474, 528, 788, 885
StyD4I	CCNGG	5 cut(s)	43, 207, 301, 589, 811
TaqI	TCGA	3 cut(s)	440, 686, 803
TasI	AATT	5 cut(s)	469, 601, 757, 824, 921
TauI	GCSGC	6 cut(s)	17, 115, 118, 121, 196, 215
TscAI	CASTG	4 cut(s)	411, 661, 667, 906
TseI	GCWGC	3 cut(s)	121, 576, 648
TspDTI	ATGAA	4 cut(s)	134, 516, 525, 707
TspGWI	ACGGA	2 cut(s)	40, 791
TspRI	CASTG	4 cut(s)	411, 661, 667, 906
Tth111I	GACNNNGTC	1 cut(s)	224
Van91I	CCANNNNNTGG	2 cut(s)	139, 644
VpaK11BI	GGWCC	4 cut(s)	226, 231, 373, 477
XceI	RCATGY	1 cut(s)	589
XcmI	CCANNNNNNNNNTGG	1 cut(s)	976
XmiI	GTMKAC	1 cut(s)	440
XspI	CTAG	5 cut(s)	458, 474, 528, 788, 885

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.