Rw2G052490

Removes the formyl group from the N-terminal Met of newly synthesized proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005371
Physical Location & Seq
Forward (+)
80422951 .. 80425678
2728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G052490.1

Sequence Viewer

Length: 822 bp
ATGGCTTGTGCGGCTTGGCTCCAGCCAATTTCCTCACCCCACGCTATCTTTCCATCTCTCTGCCGCCGCCCAATTTTTCCCGCCATCTTCCACCGCTGCACCACCTTCTCCTCCGCCGGCCAGCCACTATCCGCCGCCAACCGCTTCACTCCGGTCCACGCGCAGGCCAAACGAGGCTCCTCACTCAAAGAAGACGAAGTAGCTTCTGCCGCGGATGTTGAATTTGAGGCGCCGCTGAAAATTGTGGAGTATCCGGACCCTATACTGAGAGCAAAGAACAAGAGAATTGAATCGTTTGATGAAAATTTGAAGAAATTAGTGGAGGAAATGTTTGATATAATGTACAAAACTGATGGCATTGGGCTCTCGGCCCCGCAAGTTGGAATCAATGTGCAGCTGATGGTGTTCAATCCGGTTGGGGAGCGCGGCGAAGGGGAGGAGATTGTGCTTGTGAATCCAAGAGTTAGCAGATATTCGCAGAGAACAAGGCTCTTTGAAGAGGGTTGCTTGTCCTTCCCGGGGATTTATGCTGATGTTCAGAGACCAGAAACTGTGAAAATAGACGCACGGGACATCAGTGGTGCGAGGTTTACAGTCAACTTGTCCGGTCTTCCTGCACGGGTATTCCAGCATGAATTTGACCATTTGCAGGGTATTCTTTTCTTTGATAGAATGACTGAAGGAGTTCTTGAAAGCATCTCTGAACAGCTACAGGCATTAGAGAAGAAGTATGAAGATAAAACAGGACTTCCAAGTCCTGAAAGGATAGAAAGCCGTAAAAGGATGAAGTTGAAGGCTGCTGCTGGTTTTGGGAAATCATAG

Protein Analysis

273

Amino Acids

30.53

Weight (kDa)

6.98

Isoelectric Point (pI)

55.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pep_deformylase PF01327 79 - 227 2.7e-52 Polypeptide deformylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 753
AccB1I GGYRCC 1 cut(s) 229
AccII CGCG 3 cut(s) 161, 212, 426
AccIII TCCGGA 1 cut(s) 253
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 3 cut(s) 221, 304, 635
AcuI CTGAAG 1 cut(s) 699
AcyI GRCGYC 1 cut(s) 230
AfaI GTAC 1 cut(s) 344
AfiI CCNNNNNNNGG 4 cut(s) 163, 380, 519, 649
AgsI TTSAA 7 cut(s) 221, 290, 310, 409, 497, 692, 793
AluBI AGCT 3 cut(s) 203, 397, 709
AluI AGCT 3 cut(s) 203, 397, 709
Alw26I GTCTC 1 cut(s) 535
AlwNI CAGNNNCTG 1 cut(s) 551
Ama87I CYCGRG 1 cut(s) 517
Aor13HI TCCGGA 1 cut(s) 253
AoxI GGCC 3 cut(s) 118, 165, 369
ApeKI GCWGC 4 cut(s) 96, 394, 797, 800
ApoI RAATTY 3 cut(s) 221, 304, 635
Asp700I GAANNNNTTC 1 cut(s) 684
AspLEI GCGC 3 cut(s) 163, 232, 426
AspS9I GGNCC 3 cut(s) 154, 256, 370
AsuC2I CCSGG 2 cut(s) 518, 519
AsuHPI GGTGA 1 cut(s) 27
AvaI CYCGRG 1 cut(s) 517
AvaII GGWCC 2 cut(s) 154, 256
BanI GGYRCC 1 cut(s) 229
BanII GRGCYC 1 cut(s) 366
BbsI GAAGAC 2 cut(s) 198, 602
BbvI GCAGC 4 cut(s) 83, 406, 784, 787
BccI CCATC 4 cut(s) 61, 92, 347, 394
BceAI ACGGC 1 cut(s) 759
BciVI GTATCC 1 cut(s) 261
BcnI CCSGG 2 cut(s) 518, 519
BcoDI GTCTC 1 cut(s) 535
BfmI CTRYAG 1 cut(s) 710
BfoI RGCGCY 1 cut(s) 233
BfuI GTATCC 1 cut(s) 261
Bme1390I CCNGG 2 cut(s) 518, 519
Bme18I GGWCC 2 cut(s) 154, 256
BmeT110I CYCGRG 1 cut(s) 517
BmgT120I GGNCC 3 cut(s) 154, 256, 370
BmiI GGNNCC 5 cut(s) 20, 178, 231, 258, 372
BmrFI CCNGG 2 cut(s) 518, 519
BmsI GCATC 1 cut(s) 705
BpiI GAAGAC 2 cut(s) 198, 602
BpmI CTGGAG 1 cut(s) 5
BpuMI CCSGG 2 cut(s) 518, 519
BsaHI GRCGYC 1 cut(s) 230
BsaI GGTCTC 1 cut(s) 535
BsaJI CCNNGG 3 cut(s) 210, 517, 518
BsaWI WCCGGW 4 cut(s) 151, 253, 412, 605
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 4 cut(s) 163, 380, 519, 649
Bse118I RCCGGY 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 253
BseDI CCNNGG 3 cut(s) 210, 517, 518
BseGI GGATG 2 cut(s) 220, 789
BseLI CCNNNNNNNGG 4 cut(s) 163, 380, 519, 649
BseMII CTCAG 1 cut(s) 257
BseRI GAGGAG 3 cut(s) 100, 169, 452
BseXI GCAGC 4 cut(s) 83, 406, 784, 787
BsgI GTGCAG 3 cut(s) 82, 413, 600
Bsh1236I CGCG 3 cut(s) 161, 212, 426
BshFI GGCC 3 cut(s) 120, 167, 371
BshNI GGYRCC 1 cut(s) 229
BsiHKCI CYCGRG 1 cut(s) 517
BsiSI CCGG 6 cut(s) 117, 152, 254, 413, 518, 606
BslFI GGGAC 1 cut(s) 584
BslI CCNNNNNNNGG 4 cut(s) 163, 380, 519, 649
BsmAI GTCTC 1 cut(s) 535
BsmFI GGGAC 1 cut(s) 584
BsnI GGCC 3 cut(s) 120, 167, 371
Bso31I GGTCTC 1 cut(s) 535
BsoBI CYCGRG 1 cut(s) 517
Bsp1286I GDGCHC 1 cut(s) 366
Bsp13I TCCGGA 1 cut(s) 253
Bsp1407I TGTACA 1 cut(s) 342
BspANI GGCC 3 cut(s) 120, 167, 371
BspCNI CTCAG 1 cut(s) 258
BspEI TCCGGA 1 cut(s) 253
BspFNI CGCG 3 cut(s) 161, 212, 426
BspLI GGNNCC 5 cut(s) 20, 178, 231, 258, 372
BspT107I GGYRCC 1 cut(s) 229
BspTNI GGTCTC 1 cut(s) 535
BsrFI RCCGGY 1 cut(s) 116
BsrGI TGTACA 1 cut(s) 342
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 3 cut(s) 210, 517, 518
BssNI GRCGYC 1 cut(s) 230
Bst4CI ACNGT 2 cut(s) 553, 595
Bst6I CTCTTC 1 cut(s) 492
BstACI GRCGYC 1 cut(s) 230
BstAUI TGTACA 1 cut(s) 342
BstC8I GCNNGC 3 cut(s) 118, 122, 165
BstDEI CTNAG 1 cut(s) 266
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 2 cut(s) 220, 789
BstFNI CGCG 3 cut(s) 161, 212, 426
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 3 cut(s) 163, 232, 426
BstMAI GTCTC 1 cut(s) 535
BstMWI GCNNNNNNNGC 2 cut(s) 11, 209
BstSCI CCNGG 2 cut(s) 516, 517
BstSFI CTRYAG 1 cut(s) 710
BstUI CGCG 3 cut(s) 161, 212, 426
BstV1I GCAGC 4 cut(s) 83, 406, 784, 787
BstV2I GAAGAC 2 cut(s) 198, 602
BsuI GTATCC 1 cut(s) 261
BsuRI GGCC 3 cut(s) 120, 167, 371
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 2 cut(s) 220, 789
BtsIMutI CAGTG 1 cut(s) 583
Cac8I GCNNGC 3 cut(s) 118, 122, 165
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 3 cut(s) 163, 232, 426
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 3 cut(s) 154, 256, 370
Cfr42I CCGCGG 1 cut(s) 213
Cfr9I CCCGGG 1 cut(s) 517
CseI GACGC 1 cut(s) 572
Csp6I GTAC 1 cut(s) 343
CviAII CATG 1 cut(s) 632
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 1 cut(s) 266
DinI GGCGCC 1 cut(s) 231
DrdI GACNNNNNNGTC 1 cut(s) 753
DseDI GACNNNNNNGTC 1 cut(s) 753
EaeI YGGCCR 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 492
EciI GGCGGA 2 cut(s) 103, 121
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 535
Eco47I GGWCC 2 cut(s) 154, 256
Eco57I CTGAAG 1 cut(s) 699
Eco88I CYCGRG 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 366
EgeI GGCGCC 1 cut(s) 231
EheI GGCGCC 1 cut(s) 231
FaeI CATG 1 cut(s) 635
FaiI YATR 6 cut(s) 263, 338, 528, 633, 732, 820
FalI AAGNNNNNCTT 2 cut(s) 672, 704
FaqI GGGAC 1 cut(s) 584
FatI CATG 1 cut(s) 631
FauI CCCGC 2 cut(s) 88, 381
FokI GGATG 2 cut(s) 227, 796
FriOI GRGCYC 1 cut(s) 366
GlaI GCGC 3 cut(s) 162, 231, 425
GsuI CTGGAG 1 cut(s) 5
HaeII RGCGCY 1 cut(s) 233
HaeIII GGCC 3 cut(s) 120, 167, 371
HapII CCGG 6 cut(s) 117, 152, 254, 413, 518, 606
HgaI GACGC 1 cut(s) 572
HhaI GCGC 3 cut(s) 163, 232, 426
Hin1I GRCGYC 1 cut(s) 230
Hin1II CATG 1 cut(s) 635
Hin6I GCGC 3 cut(s) 161, 230, 424
HinP1I GCGC 3 cut(s) 161, 230, 424
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 3 cut(s) 290, 384, 454
HpaII CCGG 6 cut(s) 117, 152, 254, 413, 518, 606
HphI GGTGA 1 cut(s) 27
Hpy166II GTNNAC 3 cut(s) 157, 591, 598
Hpy188I TCNGA 2 cut(s) 540, 703
Hpy188III TCNNGA 3 cut(s) 254, 689, 758
Hpy8I GTNNAC 3 cut(s) 157, 591, 598
HpyAV CCTTC 5 cut(s) 115, 425, 523, 674, 787
HpyCH4III ACNGT 2 cut(s) 553, 595
HpyCH4V TGCA 4 cut(s) 99, 394, 617, 649
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 209
HpyF3I CTNAG 1 cut(s) 266
Hsp92I GRCGYC 1 cut(s) 230
Hsp92II CATG 1 cut(s) 635
HspAI GCGC 3 cut(s) 161, 230, 424
KasI GGCGCC 1 cut(s) 229
Kpn2I TCCGGA 1 cut(s) 253
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
KspI CCGCGG 1 cut(s) 213
LmnI GCTCC 3 cut(s) 24, 182, 421
Lsp1109I GCAGC 4 cut(s) 83, 406, 784, 787
LweI GCATC 1 cut(s) 705
MboII GAAGA 7 cut(s) 79, 203, 322, 509, 602, 736, 746
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 8 cut(s) 27, 72, 221, 240, 285, 304, 314, 635
Mly113I GGCGCC 1 cut(s) 230
MmeI TCCRAC 1 cut(s) 361
MnlI CCTC 9 cut(s) 43, 121, 167, 190, 220, 316, 430, 493, 579
MroI TCCGGA 1 cut(s) 253
MroNI GCCGGC 1 cut(s) 116
MroXI GAANNNNTTC 1 cut(s) 684
MspA1I CMGCKG 4 cut(s) 96, 212, 235, 397
MspI CCGG 6 cut(s) 117, 152, 254, 413, 518, 606
MspR9I CCNGG 2 cut(s) 518, 519
MvnI CGCG 3 cut(s) 161, 212, 426
MwoI GCNNNNNNNGC 2 cut(s) 11, 209
NaeI GCCGGC 1 cut(s) 118
NarI GGCGCC 1 cut(s) 230
NciI CCSGG 2 cut(s) 518, 519
NgoMIV GCCGGC 1 cut(s) 116
NlaIII CATG 1 cut(s) 635
NlaIV GGNNCC 5 cut(s) 20, 178, 231, 258, 372
NmeAIII GCCGAG 1 cut(s) 347
PdiI GCCGGC 1 cut(s) 118
PdmI GAANNNNTTC 1 cut(s) 684
PfeI GAWTC 3 cut(s) 290, 384, 454
PluTI GGCGCC 1 cut(s) 233
PspN4I GGNNCC 5 cut(s) 20, 178, 231, 258, 372
PspPI GGNCC 3 cut(s) 154, 256, 370
PstNI CAGNNNCTG 1 cut(s) 551
PvuII CAGCTG 1 cut(s) 397
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
SacII CCGCGG 1 cut(s) 213
Sau96I GGNCC 3 cut(s) 154, 256, 370
ScrFI CCNGG 2 cut(s) 518, 519
SduI GDGCHC 1 cut(s) 366
SetI ASST 5 cut(s) 107, 205, 399, 590, 711
SfaNI GCATC 1 cut(s) 705
SfcI CTRYAG 1 cut(s) 710
SfoI GGCGCC 1 cut(s) 231
Sfr303I CCGCGG 1 cut(s) 213
SgrBI CCGCGG 1 cut(s) 213
SinI GGWCC 2 cut(s) 154, 256
SmaI CCCGGG 1 cut(s) 519
Sse9I AATT 8 cut(s) 27, 72, 221, 240, 285, 304, 314, 635
SspDI GGCGCC 1 cut(s) 229
StyD4I CCNGG 2 cut(s) 516, 517
TaaI ACNGT 2 cut(s) 553, 595
TasI AATT 8 cut(s) 27, 72, 221, 240, 285, 304, 314, 635
TatI WGTACW 1 cut(s) 342
TauI GCSGC 7 cut(s) 14, 66, 69, 137, 212, 235, 429
TfiI GAWTC 3 cut(s) 290, 384, 454
TscAI CASTG 1 cut(s) 583
TseI GCWGC 4 cut(s) 96, 394, 797, 800
TspDTI ATGAA 4 cut(s) 315, 648, 747, 800
TspMI CCCGGG 1 cut(s) 517
TspRI CASTG 1 cut(s) 583
VpaK11BI GGWCC 2 cut(s) 154, 256
XapI RAATTY 3 cut(s) 221, 304, 635
XmaI CCCGGG 1 cut(s) 517
XmnI GAANNNNTTC 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.