Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0010934

Physical Location & Seq

Genomic Coordinates Forward (+)

80987466 .. 80990226

Gene Structure 2761 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G053140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1104 bp

ATGGATCATCATCATCATGATCAGAATAGCGAAACGGCGTCGTCGGAGCCGGCGGCCATAGAGCACAGGGCCTACGCCAGGGTGGGTCTGCTCGGCAACCCCAGCGATGTCTACTACGGCCGCACGATCTCTTTCAGCCTCGGCAACTTCTGGGCCTCCGTCCGATTACACCCCTCCGATGACCTCGTCATCCAGCCCCACCCCACCCACGACCTCGTCGTCTTCAGATCTCTCCCCCACCTGGTGAATCGGCTAGACAATGAGGGTTATTATGGCGGTGTTCGATTACTGATGGCGATTTGCAAAGTGTTTTTTGATTATTGCAAGGAAAAAGAGATCCATCTGGACTCGAGAAACTTCACTCTCTCATATGATACTAATATCCCTCGCCAGATGGGACTATCGGGGTCTAGTGCTATTGTGTGTGCAGCCCTTAGCTGCCTTCTTGACTTCTACAAGGTTAGGCATTTGATCAAAGTGGAGGTCAGGCCTGGCATTGTCCTCGGCGCGGAGAAACAGCTCGGCATTGTTGCTGGTCTTCAAGATCGGGTTGCACAGGTTTATGGAGGCCTTGTTTACATGGAATTTGATAAGGAACACATGGATACTTTGGGGCATGGCATTTACACACCCATGGATGTTTGTCTTCTGCCGCCTCTATATCTCATATATGCCGAGAATCCAAGCGATTCTGGGAAGGTACACAGTACAGTTCGCCAAAGGTGGCTCAATGGCGATGAGTTTATAATATCATCAATGAAAGAAGTAGCAGATATTGCACAAGAAGGAAGGAAAGCCTTACTCGAAAAGGACTACACTAAACTAGCAGCTCTCATGAACTCTAATTTTGATCTTAGAAGACGCATGTTTGGAGATGACGCTCTTGGTGCTTTAAACATAGAAATGGTAGAGGTCGCCCGAAGGGTGGGTGCAGCCTCGAAATTCACAGGTAGTGGAGGAGCTGTGGTTGTATTTTGCCCTGATGGTCCTTCACAAGTGAAGCTATTAGAGGATGCATGTCTAAGCGCCGGTTTTAAATTAGAACCAATTAAAGTTATTCCCTCCCTTCTAAATGAGGTTGATCTTCAAACCCTGTCAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006020 GO:0006066 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009856 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019751 GO:0022414 GO:0032501 GO:0032502 GO:0042546 GO:0044085 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0047940 GO:0048856 GO:0048868 GO:0051704 GO:0071554 GO:0071704 GO:0071840 GO:0097159 GO:1901363 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00040 ko00053 ko00520 ko01100 map00040 map00053 map00520 map01100

Pfam Domains

Protein Families

GHMP_kinases_C GHMP_kinases_N

Protein Analysis

367

Amino Acids

40.73

Weight (kDa)

5.96

Isoelectric Point (pI)

37.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GHMP_kinases_N	PF00288	112 - 190	3.9e-09	GHMP kinases N terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010934)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01640 AT3G01640 AT5G14470
fragaria_vesca	FvH4_6g51800 FvH4_6g51800
malus_domestica	MD09G1023000.v1.1 MD17G1022300.v1.1
prunus_persica	Prupe.3G293800_v2.0.a1
pyrus_communis	pycom111g01930 pycom17g01930
rosa_chinensis	RchiOBHm_Chr2g0173281
rosa_laevigata	RLG00000022176
rosa_multiflora	Rmu_sc0010961.1_g000007
rosa_roxburghii	Rroxscaffold_2G00079080
rosa_rugosa	Rorug02G0567000
rosa_samantha	Rh2AG647400 Rh2BG657900 Rh2CG623300 Rh2DG671700
rosa_wichuraiana	Rw2G053140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	746
AasI	GACNNNNNNGTC	1 cut(s)	218
AccI	GTMKAC	1 cut(s)	111
AccII	CGCG	1 cut(s)	509
AciI	CCGC	5 cut(s)	53, 121, 276, 509, 653
AclWI	GGATC	2 cut(s)	12, 331
AcoI	YGGCCR	2 cut(s)	54, 118
AcsI	RAATTY	2 cut(s)	584, 941
AcuI	CTGAAG	1 cut(s)	208
AcyI	GRCGYC	1 cut(s)	38
AdeI	CACNNNGTG	1 cut(s)	244
AfaI	GTAC	2 cut(s)	702, 709
AfiI	CCNNNNNNNGG	4 cut(s)	78, 241, 508, 925
AgsI	TTSAA	2 cut(s)	542, 1088
AjnI	CCWGG	3 cut(s)	77, 240, 490
AluBI	AGCT	5 cut(s)	438, 520, 830, 962, 1003
AluI	AGCT	5 cut(s)	438, 520, 830, 962, 1003
Alw21I	GWGCWC	1 cut(s)	66
AlwI	GGATC	2 cut(s)	12, 331
Ama87I	CYCGRG	1 cut(s)	349
AoxI	GGCC	6 cut(s)	54, 69, 118, 153, 488, 568
ApeKI	GCWGC	4 cut(s)	428, 438, 827, 932
ApoI	RAATTY	2 cut(s)	584, 941
ArsI	GACNNNNNNTTYG	2 cut(s)	468, 500
AspLEI	GCGC	2 cut(s)	509, 1028
AspS9I	GGNCC	3 cut(s)	69, 153, 986
AsuHPI	GGTGA	1 cut(s)	256
AvaI	CYCGRG	1 cut(s)	349
AvaII	GGWCC	1 cut(s)	986
BbsI	GAAGAC	4 cut(s)	214, 530, 638, 865
Bbv12I	GWGCWC	1 cut(s)	66
BbvI	GCAGC	4 cut(s)	425, 440, 839, 944
BccI	CCATC	4 cut(s)	286, 348, 388, 977
BceAI	ACGGC	2 cut(s)	51, 133
BcgI	CGANNNNNNTGC	2 cut(s)	484, 518
BciT130I	CCWGG	3 cut(s)	79, 242, 492
BciVI	GTATCC	1 cut(s)	598
BclI	TGATCA	2 cut(s)	19, 471
BfaI	CTAG	3 cut(s)	254, 411, 824
BfoI	RGCGCY	1 cut(s)	1029
BfuI	GTATCC	1 cut(s)	598
BglII	AGATCT	1 cut(s)	227
BisI	GCNGC	7 cut(s)	54, 121, 429, 439, 653, 828, 933
BlsI	GCNGC	7 cut(s)	55, 122, 430, 440, 654, 829, 934
Bme1390I	CCNGG	3 cut(s)	79, 242, 492
Bme18I	GGWCC	1 cut(s)	986
BmeT110I	CYCGRG	1 cut(s)	349
BmgT120I	GGNCC	3 cut(s)	69, 153, 986
BmiI	GGNNCC	1 cut(s)	48
BmrFI	CCNGG	3 cut(s)	79, 242, 492
BmsI	GCATC	1 cut(s)	1003
BpiI	GAAGAC	4 cut(s)	214, 530, 638, 865
Bpu10I	CCTNAGC	1 cut(s)	434
BsaBI	GATNNNNATC	1 cut(s)	9
BsaHI	GRCGYC	1 cut(s)	38
BsaJI	CCNNGG	4 cut(s)	78, 139, 502, 633
BsaXI	ACNNNNNCTCC	6 cut(s)	558, 588, 948, 951, 978, 981
Bsc4I	CCNNNNNNNGG	4 cut(s)	78, 241, 508, 925
Bse118I	RCCGGY	2 cut(s)	49, 1028
Bse8I	GATNNNNATC	1 cut(s)	9
BseBI	CCWGG	3 cut(s)	79, 242, 492
BseDI	CCNNGG	4 cut(s)	78, 139, 502, 633
BseGI	GGATG	3 cut(s)	189, 643, 1018
BseJI	GATNNNNATC	1 cut(s)	9
BseLI	CCNNNNNNNGG	4 cut(s)	78, 241, 508, 925
BseRI	GAGGAG	1 cut(s)	972
BseX3I	CGGCCG	1 cut(s)	118
BseXI	GCAGC	4 cut(s)	425, 440, 839, 944
BseYI	CCCAGC	1 cut(s)	101
BsgI	GTGCAG	2 cut(s)	447, 951
Bsh1236I	CGCG	1 cut(s)	509
Bsh1285I	CGRYCG	1 cut(s)	121
BshFI	GGCC	6 cut(s)	56, 71, 120, 155, 490, 570
BsiEI	CGRYCG	1 cut(s)	121
BsiHKAI	GWGCWC	1 cut(s)	66
BsiHKCI	CYCGRG	1 cut(s)	349
BsiSI	CCGG	2 cut(s)	50, 1029
BslFI	GGGAC	1 cut(s)	411
BslI	CCNNNNNNNGG	4 cut(s)	78, 241, 508, 925
BsmFI	GGGAC	1 cut(s)	411
BsnI	GGCC	6 cut(s)	56, 71, 120, 155, 490, 570
BsoBI	CYCGRG	1 cut(s)	349
Bsp1286I	GDGCHC	1 cut(s)	66
Bsp143I	GATC	9 cut(s)	4, 19, 126, 227, 336, 471, 544, 850, 1081
Bsp19I	CCATGG	1 cut(s)	633
BspACI	CCGC	5 cut(s)	53, 121, 276, 509, 653
BspANI	GGCC	6 cut(s)	56, 71, 120, 155, 490, 570
BspFNI	CGCG	1 cut(s)	509
BspHI	TCATGA	2 cut(s)	16, 834
BspLI	GGNNCC	1 cut(s)	48
BspPI	GGATC	2 cut(s)	12, 331
BsrFI	RCCGGY	2 cut(s)	49, 1028
BssAI	RCCGGY	2 cut(s)	49, 1028
BssECI	CCNNGG	4 cut(s)	78, 139, 502, 633
BssMI	GATC	9 cut(s)	4, 19, 126, 227, 336, 471, 544, 850, 1081
BssNI	GRCGYC	1 cut(s)	38
BssT1I	CCWWGG	1 cut(s)	633
Bst2UI	CCWGG	3 cut(s)	79, 242, 492
Bst4CI	ACNGT	2 cut(s)	707, 712
BstACI	GRCGYC	1 cut(s)	38
BstAPI	GCANNNNNTGC	1 cut(s)	776
BstC8I	GCNNGC	1 cut(s)	51
BstDEI	CTNAG	3 cut(s)	434, 854, 1022
BstDSI	CCRYGG	1 cut(s)	633
BstENI	CCTNNNNNAGG	1 cut(s)	76
BstF5I	GGATG	3 cut(s)	189, 643, 1018
BstFNI	CGCG	1 cut(s)	509
BstH2I	RGCGCY	1 cut(s)	1029
BstHHI	GCGC	2 cut(s)	509, 1028
BstKTI	GATC	9 cut(s)	7, 22, 129, 230, 339, 474, 547, 853, 1084
BstMBI	GATC	9 cut(s)	4, 19, 126, 227, 336, 471, 544, 850, 1081
BstMCI	CGRYCG	1 cut(s)	121
BstMWI	GCNNNNNNNGC	3 cut(s)	102, 776, 887
BstNI	CCWGG	3 cut(s)	79, 242, 492
BstNSI	RCATGY	2 cut(s)	868, 1020
BstSCI	CCNGG	3 cut(s)	77, 240, 490
BstUI	CGCG	1 cut(s)	509
BstV1I	GCAGC	4 cut(s)	425, 440, 839, 944
BstV2I	GAAGAC	4 cut(s)	214, 530, 638, 865
BstX2I	RGATCY	2 cut(s)	227, 336
BstYI	RGATCY	2 cut(s)	227, 336
BstZI	CGGCCG	1 cut(s)	118
BsuI	GTATCC	1 cut(s)	598
BsuRI	GGCC	6 cut(s)	56, 71, 120, 155, 490, 570
BtgI	CCRYGG	1 cut(s)	633
BtgZI	GCGATG	2 cut(s)	120, 750
BtsCI	GGATG	3 cut(s)	189, 643, 1018
Cac8I	GCNNGC	1 cut(s)	51
CciI	TCATGA	2 cut(s)	16, 834
CfoI	GCGC	2 cut(s)	509, 1028
Cfr10I	RCCGGY	2 cut(s)	49, 1028
Cfr13I	GGNCC	3 cut(s)	69, 153, 986
CseI	GACGC	3 cut(s)	27, 870, 887
CsiI	ACCWGGT	1 cut(s)	240
Csp6I	GTAC	2 cut(s)	701, 708
CviAII	CATG	8 cut(s)	17, 580, 601, 617, 634, 835, 865, 1017
CviQI	GTAC	2 cut(s)	701, 708
DdeI	CTNAG	3 cut(s)	434, 854, 1022
DpnI	GATC	9 cut(s)	6, 21, 128, 229, 338, 473, 546, 852, 1083
DpnII	GATC	9 cut(s)	4, 19, 126, 227, 336, 471, 544, 850, 1081
DraI	TTTAAA	2 cut(s)	894, 1036
DraIII	CACNNNGTG	1 cut(s)	244
DrdI	GACNNNNNNGTC	1 cut(s)	218
DseDI	GACNNNNNNGTC	1 cut(s)	218
EaeI	YGGCCR	2 cut(s)	54, 118
EagI	CGGCCG	1 cut(s)	118
EclXI	CGGCCG	1 cut(s)	118
Eco130I	CCWWGG	1 cut(s)	633
Eco147I	AGGCCT	2 cut(s)	490, 570
Eco47I	GGWCC	1 cut(s)	986
Eco52I	CGGCCG	1 cut(s)	118
Eco57I	CTGAAG	1 cut(s)	208
Eco88I	CYCGRG	1 cut(s)	349
EcoNI	CCTNNNNNAGG	1 cut(s)	76
EcoO109I	RGGNCCY	1 cut(s)	69
EcoRII	CCWGG	3 cut(s)	77, 240, 490
EcoT14I	CCWWGG	1 cut(s)	633
EcoT22I	ATGCAT	1 cut(s)	1018
ErhI	CCWWGG	1 cut(s)	633
FaeI	CATG	8 cut(s)	20, 583, 604, 620, 637, 838, 868, 1020
FaqI	GGGAC	1 cut(s)	411
FatI	CATG	8 cut(s)	16, 579, 600, 616, 633, 834, 864, 1016
FauNDI	CATATG	1 cut(s)	370
FbaI	TGATCA	2 cut(s)	19, 471
FblI	GTMKAC	1 cut(s)	111
Fnu4HI	GCNGC	7 cut(s)	54, 121, 429, 439, 653, 828, 933
FokI	GGATG	3 cut(s)	176, 650, 1025
Fsp4HI	GCNGC	7 cut(s)	54, 121, 429, 439, 653, 828, 933
FspBI	CTAG	3 cut(s)	254, 411, 824
GlaI	GCGC	2 cut(s)	508, 1027
GluI	GCNGC	7 cut(s)	54, 121, 429, 439, 653, 828, 933
GsaI	CCCAGC	1 cut(s)	105
HaeII	RGCGCY	1 cut(s)	1029
HaeIII	GGCC	6 cut(s)	56, 71, 120, 155, 490, 570
HapII	CCGG	2 cut(s)	50, 1029
HgaI	GACGC	3 cut(s)	27, 870, 887
HhaI	GCGC	2 cut(s)	509, 1028
Hin1I	GRCGYC	1 cut(s)	38
Hin1II	CATG	8 cut(s)	20, 583, 604, 620, 637, 838, 868, 1020
Hin6I	GCGC	2 cut(s)	507, 1026
HinP1I	GCGC	2 cut(s)	507, 1026
HinfI	GANTC	4 cut(s)	247, 347, 679, 689
HpaII	CCGG	2 cut(s)	50, 1029
HphI	GGTGA	1 cut(s)	256
Hpy166II	GTNNAC	3 cut(s)	112, 577, 703
Hpy188I	TCNGA	5 cut(s)	24, 46, 164, 178, 227
Hpy188III	TCNNGA	6 cut(s)	17, 344, 351, 446, 542, 835
Hpy8I	GTNNAC	3 cut(s)	112, 577, 703
Hpy99I	CGWCG	3 cut(s)	43, 46, 221
HpyAV	CCTTC	7 cut(s)	452, 691, 779, 783, 915, 999, 1076
HpyCH4III	ACNGT	2 cut(s)	707, 712
HpyCH4V	TGCA	7 cut(s)	303, 324, 428, 554, 779, 932, 1016
HpyF10VI	GCNNNNNNNGC	3 cut(s)	102, 776, 887
HpyF3I	CTNAG	3 cut(s)	434, 854, 1022
Hsp92I	GRCGYC	1 cut(s)	38
Hsp92II	CATG	8 cut(s)	20, 583, 604, 620, 637, 838, 868, 1020
HspAI	GCGC	2 cut(s)	507, 1026
KroI	GCCGGC	1 cut(s)	49
KroNI	GCCGGC	1 cut(s)	51
Ksp22I	TGATCA	2 cut(s)	19, 471
Kzo9I	GATC	9 cut(s)	4, 19, 126, 227, 336, 471, 544, 850, 1081
LmnI	GCTCC	2 cut(s)	46, 959
Lsp1109I	GCAGC	4 cut(s)	425, 440, 839, 944
LweI	GCATC	1 cut(s)	1003
MabI	ACCWGGT	1 cut(s)	240
MaeI	CTAG	3 cut(s)	254, 411, 824
MalI	GATC	9 cut(s)	6, 21, 128, 229, 338, 473, 546, 852, 1083
MboI	GATC	9 cut(s)	4, 19, 126, 227, 336, 471, 544, 850, 1081
MboII	GAAGA	5 cut(s)	214, 530, 638, 870, 1076
MflI	RGATCY	2 cut(s)	227, 336
MhlI	GDGCHC	1 cut(s)	66
MluCI	AATT	5 cut(s)	584, 844, 941, 1037, 1047
MlyI	GAGTC	1 cut(s)	341
MmeI	TCCRAC	1 cut(s)	24
Mph1103I	ATGCAT	1 cut(s)	1018
MroNI	GCCGGC	1 cut(s)	49
MseI	TTAA	3 cut(s)	893, 1035, 1050
MslI	CAYNNNNRTG	3 cut(s)	15, 632, 902
MspI	CCGG	2 cut(s)	50, 1029
MspR9I	CCNGG	3 cut(s)	79, 242, 492
MvaI	CCWGG	3 cut(s)	79, 242, 492
MvnI	CGCG	1 cut(s)	509
MwoI	GCNNNNNNNGC	3 cut(s)	102, 776, 887
NaeI	GCCGGC	1 cut(s)	51
NcoI	CCATGG	1 cut(s)	633
NdeI	CATATG	1 cut(s)	370
NdeII	GATC	9 cut(s)	4, 19, 126, 227, 336, 471, 544, 850, 1081
NgoMIV	GCCGGC	1 cut(s)	49
NlaIII	CATG	8 cut(s)	20, 583, 604, 620, 637, 838, 868, 1020
NlaIV	GGNNCC	1 cut(s)	48
NmeAIII	GCCGAG	5 cut(s)	72, 120, 483, 501, 700
NsiI	ATGCAT	1 cut(s)	1018
NspI	RCATGY	2 cut(s)	868, 1020
PaeR7I	CTCGAG	1 cut(s)	349
PagI	TCATGA	2 cut(s)	16, 834
PceI	AGGCCT	2 cut(s)	490, 570
PcsI	WCGNNNNNNNCGW	1 cut(s)	216
PdiI	GCCGGC	1 cut(s)	51
PfeI	GAWTC	3 cut(s)	247, 679, 689
PflFI	GACNNNGTC	2 cut(s)	185, 215
PkrI	GCNGC	7 cut(s)	55, 122, 430, 440, 654, 829, 934
PleI	GAGTC	1 cut(s)	341
PpsI	GAGTC	1 cut(s)	341
PsiI	TTATAA	1 cut(s)	746
Psp6I	CCWGG	3 cut(s)	77, 240, 490
PspFI	CCCAGC	1 cut(s)	101
PspGI	CCWGG	3 cut(s)	77, 240, 490
PspN4I	GGNNCC	1 cut(s)	48
PspPI	GGNCC	3 cut(s)	69, 153, 986
PsuI	RGATCY	2 cut(s)	227, 336
PsyI	GACNNNGTC	2 cut(s)	185, 215
RsaI	GTAC	2 cut(s)	702, 709
RsaNI	GTAC	2 cut(s)	701, 708
RseI	CAYNNNNRTG	3 cut(s)	15, 632, 902
SaqAI	TTAA	3 cut(s)	893, 1035, 1050
SatI	GCNGC	7 cut(s)	54, 121, 429, 439, 653, 828, 933
Sau3AI	GATC	9 cut(s)	4, 19, 126, 227, 336, 471, 544, 850, 1081
Sau96I	GGNCC	3 cut(s)	69, 153, 986
SchI	GAGTC	1 cut(s)	341
ScrFI	CCNGG	3 cut(s)	79, 242, 492
SduI	GDGCHC	1 cut(s)	66
SexAI	ACCWGGT	1 cut(s)	240
SfaNI	GCATC	1 cut(s)	1003
Sfr274I	CTCGAG	1 cut(s)	349
SinI	GGWCC	1 cut(s)	986
SlaI	CTCGAG	1 cut(s)	349
SmiMI	CAYNNNNRTG	3 cut(s)	15, 632, 902
SmlI	CTYRAG	1 cut(s)	349
SmoI	CTYRAG	1 cut(s)	349
Sse9I	AATT	5 cut(s)	584, 844, 941, 1037, 1047
SseBI	AGGCCT	2 cut(s)	490, 570
SsiI	CCGC	5 cut(s)	53, 121, 276, 509, 653
SspMI	CTAG	3 cut(s)	254, 411, 824
StuI	AGGCCT	2 cut(s)	490, 570
StyD4I	CCNGG	3 cut(s)	77, 240, 490
StyI	CCWWGG	1 cut(s)	633
TaaI	ACNGT	2 cut(s)	707, 712
TaqI	TCGA	4 cut(s)	283, 350, 804, 938
TasI	AATT	5 cut(s)	584, 844, 941, 1037, 1047
TatI	WGTACW	1 cut(s)	707
TauI	GCSGC	3 cut(s)	56, 123, 655
TfiI	GAWTC	3 cut(s)	247, 679, 689
Tru1I	TTAA	3 cut(s)	893, 1035, 1050
Tru9I	TTAA	3 cut(s)	893, 1035, 1050
TseI	GCWGC	4 cut(s)	428, 438, 827, 932
TspDTI	ATGAA	2 cut(s)	773, 851
TspGWI	ACGGA	1 cut(s)	148
Tth111I	GACNNNGTC	2 cut(s)	185, 215
VpaK11BI	GGWCC	1 cut(s)	986
XagI	CCTNNNNNAGG	1 cut(s)	76
XapI	RAATTY	2 cut(s)	584, 941
XceI	RCATGY	2 cut(s)	868, 1020
XhoI	CTCGAG	1 cut(s)	349
XmiI	GTMKAC	1 cut(s)	111
XspI	CTAG	3 cut(s)	254, 411, 824
Zsp2I	ATGCAT	1 cut(s)	1018

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.