Rw4G004170

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008125
Physical Location & Seq
Forward (+)
8350405 .. 8367479
17075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004170.1

Sequence Viewer

Length: 1695 bp
ATGAGCTCTCACCCAGATGTCGCTAACGCGCCAGCCGGAGAAGTAAATGTAACAAAGAAATGGGAAAGAATGGTTTCATTTTGGAATAAGATTTCTGTGCCTGTATGTATGATTGCAGTCTCAATGGATCCTTTGTTTTTTTACATTGCATTCCTTGATCGTGAAAAAGTGTGCCTCGGAACAGACAAAAGGCTGAGAACCGAAGTCCTCATTACGCGATCACTTGCAGATTTCACGTTCCTATGTCATATCATGTATCAACTTTGGGACGCAGCGCCGGTAACGACAAACAACTCAACAACCTTCAACTGGTTTGGACACGCCGAGGCAATAGCTAAGAATTTATCATGGAGCTCATTCCTAATTGACATTTTCGCTATTCTTCCAATTCCACACATGCTAATATTAGCTGTGCTTTATGCTCCCAATTATGGATTTTCAGAGAACAAAATGAATCTGAATTACATCATTCTCGCGCAATATGTGCCAAGGATGTACCGCATCTTCATCTCGGTTAGGCTGTTAAGAAAAAATAGTAGCGTGTGGCTCAAAGCTGCATTCTATTTTTTTATGTACATCATTTCAAGTCATGTAATTGGAGCATTTTGGCACTTCAGTTCTGTTCAAATCGAAGCAGTATGTTGGCACTGGGCCCTCAAAGGAAGTGTCAGTTTCCAATGTGATGACCCTCTTCTATCGGAAAGCAATAAGAACATCATTAGTAGACTGTGCAGTACTGAGAGATATGATTATGGATCATTTATGGAGACAATTCAAACCGGCTTCACAGGACATAGAAAATTCCGCGAAAAGTTATTTTACTTTGTGTGGTGGGGACTTAAAAATCTGAGTAAGTATGGTACAAACCTCAATACAAGTGACTTCATATGGGAAAATTGCTTTGCAATTCTTGTTTCTGGTATTGGCTTGGTACTGTTTGCTTTACTGATTGGAAATATGCAGGAGTGCTTCCAATCGGCGGAGGAAATGAAAGTGATAACCGATAAGGTTCAGTTGAAAGAGAGAGAATTACATGATTGGATGAAAAAACATTGTCTGCTTGAGTCCGGAGATACTCGGAAGTGGAAGACACAAATTATGACAAATATAACAAATAGAATGAAAGAAAACAAAGATGCTGATTTGGAAAATCTATTCTCTCTAATTCCCTGGAATTCCATGGATTGTCTAAAGTCACTAAAGTATACTCTCTGCATGAGTATGCTAAAAAAAGTACAGGTACAGGCACAGGTACAGAAACCAATGCCTAAATATTTGGATGAAGAGGGGTTGAAAACAATCTGCGACCATCTCAAGCCAGTGACCTACAATGAAAAAACAATTGTTTTTAAAATGGGAGAACCACTTGATAGCATGCTCTTAGTTCTGGAAGGCACCATGGTGATCTACACAACTGGGACTACTACTACTACTACTGTTAGTAGTACTCCTGGAGAAATTACAGGTACCTCATCAACGGTGCCGAGGTCCCTTGAGAATGACAGTGTTTATGGACAAGAACTTTTAAGTTGGGCATCACAAGGCAGAGACGACGTAACTTATGCAACCCTTCCTATCTTGACCGAAAATGTGAAATGTCTGTCACAAGTAGAAGGGTTTATCCTCTCGGCCAAGGACTTGAAAACTCTAGTTTTCGAGGACAAGTTACAATGGAAATCCGACAATGTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

564

Amino Acids

64.78

Weight (kDa)

6.83

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 30 - 329 2.5e-07 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1466
AccB1I GGYRCC 3 cut(s) 1394, 1466, 1480
AccI GTMKAC 2 cut(s) 724, 1205
AccII CGCG 4 cut(s) 29, 217, 476, 807
AccIII TCCGGA 1 cut(s) 1067
AciI CCGC 3 cut(s) 499, 805, 980
AclWI GGATC 3 cut(s) 122, 135, 763
AcoI YGGCCR 1 cut(s) 1629
AcsI RAATTY 3 cut(s) 340, 800, 1174
AcuI CTGAAG 1 cut(s) 598
AfiI CCNNNNNNNGG 3 cut(s) 309, 431, 979
AgsI TTSAA 7 cut(s) 307, 585, 626, 776, 1018, 1294, 1642
AjnI CCWGG 2 cut(s) 1169, 1450
AleI CACNNNNGTG 1 cut(s) 1400
AluBI AGCT 5 cut(s) 6, 335, 354, 410, 554
AluI AGCT 5 cut(s) 6, 335, 354, 410, 554
Alw21I GWGCWC 2 cut(s) 8, 356
Alw26I GTCTC 3 cut(s) 124, 761, 1542
AlwI GGATC 3 cut(s) 122, 135, 763
Aor13HI TCCGGA 1 cut(s) 1067
AoxI GGCC 2 cut(s) 651, 1629
ApaI GGGCCC 1 cut(s) 655
ApeKI GCWGC 2 cut(s) 272, 554
ApoI RAATTY 3 cut(s) 340, 800, 1174
ArsI GACNNNNNNTTYG 2 cut(s) 651, 683
Asp700I GAANNNNTTC 1 cut(s) 73
Asp718I GGTACC 1 cut(s) 1466
AspLEI GCGC 3 cut(s) 31, 277, 478
AspS9I GGNCC 3 cut(s) 651, 652, 1488
AsuHPI GGTGA 1 cut(s) 1414
AvaII GGWCC 1 cut(s) 1488
BaeGI GKGCMC 1 cut(s) 655
BamHI GGATCC 1 cut(s) 127
BanI GGYRCC 3 cut(s) 1394, 1466, 1480
BanII GRGCYC 3 cut(s) 8, 356, 655
BarI GAAGNNNNNNTAC 2 cut(s) 488, 520
BbsI GAAGAC 1 cut(s) 1094
Bbv12I GWGCWC 2 cut(s) 8, 356
BbvI GCAGC 2 cut(s) 284, 541
BccI CCATC 1 cut(s) 1317
BciT130I CCWGG 2 cut(s) 1171, 1452
BcoDI GTCTC 3 cut(s) 124, 761, 1542
BfaI CTAG 1 cut(s) 1649
BfoI RGCGCY 1 cut(s) 278
BisI GCNGC 2 cut(s) 273, 555
BlsI GCNGC 2 cut(s) 274, 556
BmcAI AGTACT 2 cut(s) 736, 1447
Bme1390I CCNGG 2 cut(s) 1171, 1452
Bme18I GGWCC 1 cut(s) 1488
BmgT120I GGNCC 3 cut(s) 651, 652, 1488
BmiI GGNNCC 6 cut(s) 129, 653, 1396, 1468, 1482, 1490
BmrFI CCNGG 2 cut(s) 1171, 1452
BmrI ACTGGG 2 cut(s) 658, 1425
BmsI GCATC 3 cut(s) 510, 1126, 1544
BmuI ACTGGG 2 cut(s) 658, 1425
BpiI GAAGAC 1 cut(s) 1094
BpmI CTGGAG 1 cut(s) 1473
BpuEI CTTGAG 3 cut(s) 1082, 1298, 1514
BsaJI CCNNGG 8 cut(s) 175, 324, 488, 1169, 1179, 1398, 1484, 1632
BsaWI WCCGGW 1 cut(s) 1067
Bsc4I CCNNNNNNNGG 3 cut(s) 309, 431, 979
Bse118I RCCGGY 2 cut(s) 277, 779
Bse1I ACTGG 4 cut(s) 314, 653, 1319, 1420
Bse3DI GCAATG 1 cut(s) 144
BseAI TCCGGA 1 cut(s) 1067
BseBI CCWGG 2 cut(s) 1171, 1452
BseDI CCNNGG 8 cut(s) 175, 324, 488, 1169, 1179, 1398, 1484, 1632
BseGI GGATG 3 cut(s) 498, 1047, 1285
BseLI CCNNNNNNNGG 3 cut(s) 309, 431, 979
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 3 cut(s) 185, 729, 839
BseNI ACTGG 4 cut(s) 314, 653, 1319, 1420
BseSI GKGCMC 1 cut(s) 655
BseXI GCAGC 2 cut(s) 284, 541
BsgI GTGCAG 1 cut(s) 751
Bsh1236I CGCG 4 cut(s) 29, 217, 476, 807
BshFI GGCC 2 cut(s) 653, 1631
BshNI GGYRCC 3 cut(s) 1394, 1466, 1480
BsiHKAI GWGCWC 2 cut(s) 8, 356
BsiSI CCGG 4 cut(s) 36, 278, 780, 1068
BslFI GGGAC 4 cut(s) 281, 849, 1432, 1474
BslI CCNNNNNNNGG 3 cut(s) 309, 431, 979
BsmAI GTCTC 3 cut(s) 124, 761, 1542
BsmBI CGTCTC 1 cut(s) 1542
BsmFI GGGAC 4 cut(s) 281, 849, 1432, 1474
BsmI GAATGC 2 cut(s) 149, 557
BsnI GGCC 2 cut(s) 653, 1631
Bsp120I GGGCCC 1 cut(s) 651
Bsp1286I GDGCHC 3 cut(s) 8, 356, 655
Bsp13I TCCGGA 1 cut(s) 1067
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 5 cut(s) 127, 157, 218, 755, 1404
Bsp19I CCATGG 2 cut(s) 1179, 1398
BspACI CCGC 3 cut(s) 499, 805, 980
BspANI GGCC 2 cut(s) 653, 1631
BspCNI CTCAG 3 cut(s) 186, 730, 840
BspEI TCCGGA 1 cut(s) 1067
BspFNI CGCG 4 cut(s) 29, 217, 476, 807
BspLI GGNNCC 6 cut(s) 129, 653, 1396, 1468, 1482, 1490
BspPI GGATC 3 cut(s) 122, 135, 763
BspT107I GGYRCC 3 cut(s) 1394, 1466, 1480
BsrDI GCAATG 1 cut(s) 144
BsrFI RCCGGY 2 cut(s) 277, 779
BsrGI TGTACA 1 cut(s) 573
BsrI ACTGG 4 cut(s) 314, 653, 1319, 1420
BssAI RCCGGY 2 cut(s) 277, 779
BssECI CCNNGG 8 cut(s) 175, 324, 488, 1169, 1179, 1398, 1484, 1632
BssMI GATC 5 cut(s) 127, 157, 218, 755, 1404
BssNAI GTATAC 1 cut(s) 1206
BssT1I CCWWGG 4 cut(s) 488, 1179, 1398, 1632
Bst1107I GTATAC 1 cut(s) 1206
Bst2UI CCWGG 2 cut(s) 1171, 1452
Bst4CI ACNGT 5 cut(s) 729, 936, 1438, 1480, 1505
Bst6I CTCTTC 2 cut(s) 696, 1278
BstAPI GCANNNNNTGC 1 cut(s) 484
BstAUI TGTACA 1 cut(s) 573
BstC8I GCNNGC 2 cut(s) 33, 1376
BstDEI CTNAG 5 cut(s) 194, 336, 738, 848, 1381
BstDSI CCRYGG 2 cut(s) 1179, 1398
BstF5I GGATG 3 cut(s) 498, 1047, 1285
BstFNI CGCG 4 cut(s) 29, 217, 476, 807
BstH2I RGCGCY 1 cut(s) 278
BstHHI GCGC 3 cut(s) 31, 277, 478
BstKTI GATC 5 cut(s) 130, 160, 221, 758, 1407
BstMAI GTCTC 3 cut(s) 124, 761, 1542
BstMBI GATC 5 cut(s) 127, 157, 218, 755, 1404
BstMWI GCNNNNNNNGC 1 cut(s) 484
BstNI CCWGG 2 cut(s) 1171, 1452
BstNSI RCATGY 2 cut(s) 400, 1378
BstSCI CCNGG 2 cut(s) 1169, 1450
BstSLI GKGCMC 1 cut(s) 655
BstUI CGCG 4 cut(s) 29, 217, 476, 807
BstV1I GCAGC 2 cut(s) 284, 541
BstV2I GAAGAC 1 cut(s) 1094
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
BstZ17I GTATAC 1 cut(s) 1206
BsuRI GGCC 2 cut(s) 653, 1631
BtgI CCRYGG 2 cut(s) 1179, 1398
BtsCI GGATG 3 cut(s) 498, 1047, 1285
BtsIMutI CAGTG 3 cut(s) 646, 1326, 1510
Cac8I GCNNGC 2 cut(s) 33, 1376
CfoI GCGC 3 cut(s) 31, 277, 478
Cfr10I RCCGGY 2 cut(s) 277, 779
Cfr13I GGNCC 3 cut(s) 651, 652, 1488
CseI GACGC 1 cut(s) 278
CviAII CATG 9 cut(s) 253, 348, 397, 590, 1034, 1180, 1216, 1375, 1399
DdeI CTNAG 5 cut(s) 194, 336, 738, 848, 1381
DpnI GATC 5 cut(s) 129, 159, 220, 757, 1406
DpnII GATC 5 cut(s) 127, 157, 218, 755, 1404
DraI TTTAAA 1 cut(s) 1351
EaeI YGGCCR 1 cut(s) 1629
Eam1104I CTCTTC 2 cut(s) 696, 1278
EarI CTCTTC 2 cut(s) 696, 1278
EciI GGCGGA 1 cut(s) 995
Ecl136II GAGCTC 2 cut(s) 6, 354
Eco130I CCWWGG 4 cut(s) 488, 1179, 1398, 1632
Eco24I GRGCYC 3 cut(s) 8, 356, 655
Eco47I GGWCC 1 cut(s) 1488
Eco53kI GAGCTC 2 cut(s) 6, 354
Eco57I CTGAAG 1 cut(s) 598
EcoICRI GAGCTC 2 cut(s) 6, 354
EcoO109I RGGNCCY 2 cut(s) 652, 1488
EcoRI GAATTC 1 cut(s) 1174
EcoRII CCWGG 2 cut(s) 1169, 1450
EcoT14I CCWWGG 4 cut(s) 488, 1179, 1398, 1632
EcoT38I GRGCYC 3 cut(s) 8, 356, 655
ErhI CCWWGG 4 cut(s) 488, 1179, 1398, 1632
Esp3I CGTCTC 1 cut(s) 1542
FaeI CATG 9 cut(s) 256, 351, 400, 593, 1037, 1183, 1219, 1378, 1402
FaqI GGGAC 4 cut(s) 281, 849, 1432, 1474
FatI CATG 9 cut(s) 252, 347, 396, 589, 1033, 1179, 1215, 1374, 1398
FauNDI CATATG 1 cut(s) 887
FblI GTMKAC 2 cut(s) 724, 1205
Fnu4HI GCNGC 2 cut(s) 273, 555
FokI GGATG 3 cut(s) 505, 1054, 1292
FriOI GRGCYC 3 cut(s) 8, 356, 655
Fsp4HI GCNGC 2 cut(s) 273, 555
FspBI CTAG 1 cut(s) 1649
GlaI GCGC 3 cut(s) 30, 276, 477
GluI GCNGC 2 cut(s) 273, 555
GsuI CTGGAG 1 cut(s) 1473
HaeII RGCGCY 1 cut(s) 278
HaeIII GGCC 2 cut(s) 653, 1631
HapII CCGG 4 cut(s) 36, 278, 780, 1068
HgaI GACGC 1 cut(s) 278
HhaI GCGC 3 cut(s) 31, 277, 478
Hin1II CATG 9 cut(s) 256, 351, 400, 593, 1037, 1183, 1219, 1378, 1402
Hin6I GCGC 3 cut(s) 29, 275, 476
HinP1I GCGC 3 cut(s) 29, 275, 476
HinfI GANTC 2 cut(s) 454, 1064
HpaII CCGG 4 cut(s) 36, 278, 780, 1068
HphI GGTGA 1 cut(s) 1414
Hpy166II GTNNAC 2 cut(s) 725, 1206
Hpy188I TCNGA 7 cut(s) 179, 442, 459, 700, 849, 1080, 1681
Hpy188III TCNNGA 4 cut(s) 161, 1068, 1388, 1579
Hpy8I GTNNAC 2 cut(s) 725, 1206
Hpy99I CGWCG 1 cut(s) 1556
HpyAV CCTTC 4 cut(s) 313, 1385, 1580, 1607
HpyCH4III ACNGT 5 cut(s) 729, 936, 1438, 1480, 1505
HpyCH4IV ACGT 2 cut(s) 236, 1554
HpyCH4V TGCA 9 cut(s) 116, 149, 227, 557, 732, 905, 961, 1215, 1565
HpyF10VI GCNNNNNNNGC 1 cut(s) 484
HpyF3I CTNAG 5 cut(s) 194, 336, 738, 848, 1381
HpySE526I ACGT 2 cut(s) 236, 1554
Hsp92II CATG 9 cut(s) 256, 351, 400, 593, 1037, 1183, 1219, 1378, 1402
HspAI GCGC 3 cut(s) 29, 275, 476
Kpn2I TCCGGA 1 cut(s) 1067
KpnI GGTACC 1 cut(s) 1470
Kzo9I GATC 5 cut(s) 127, 157, 218, 755, 1404
LmnI GCTCC 3 cut(s) 351, 427, 599
Lsp1109I GCAGC 2 cut(s) 284, 541
LweI GCATC 3 cut(s) 510, 1126, 1544
MaeI CTAG 1 cut(s) 1649
MaeII ACGT 2 cut(s) 236, 1554
MaeIII GTNAC 8 cut(s) 49, 280, 878, 1194, 1321, 1555, 1602, 1665
MalI GATC 5 cut(s) 129, 159, 220, 757, 1406
MboI GATC 5 cut(s) 127, 157, 218, 755, 1404
MboII GAAGA 5 cut(s) 374, 496, 683, 1099, 1295
MfeI CAATTG 1 cut(s) 1341
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 3 cut(s) 8, 356, 655
MlyI GAGTC 1 cut(s) 1073
MroI TCCGGA 1 cut(s) 1067
MroXI GAANNNNTTC 1 cut(s) 73
MseI TTAA 4 cut(s) 524, 840, 1350, 1526
MslI CAYNNNNRTG 3 cut(s) 15, 1220, 1400
MspI CCGG 4 cut(s) 36, 278, 780, 1068
MspR9I CCNGG 2 cut(s) 1171, 1452
MunI CAATTG 1 cut(s) 1341
Mva1269I GAATGC 2 cut(s) 149, 557
MvaI CCWGG 2 cut(s) 1171, 1452
MvnI CGCG 4 cut(s) 29, 217, 476, 807
MwoI GCNNNNNNNGC 1 cut(s) 484
NcoI CCATGG 2 cut(s) 1179, 1398
NdeI CATATG 1 cut(s) 887
NdeII GATC 5 cut(s) 127, 157, 218, 755, 1404
NlaIII CATG 9 cut(s) 256, 351, 400, 593, 1037, 1183, 1219, 1378, 1402
NlaIV GGNNCC 6 cut(s) 129, 653, 1396, 1468, 1482, 1490
NmeAIII GCCGAG 3 cut(s) 349, 1509, 1607
NmuCI GTSAC 4 cut(s) 878, 1194, 1321, 1602
NspI RCATGY 2 cut(s) 400, 1378
OliI CACNNNNGTG 1 cut(s) 1400
PaeI GCATGC 1 cut(s) 1378
PctI GAATGC 2 cut(s) 149, 557
PdmI GAANNNNTTC 1 cut(s) 73
PfeI GAWTC 1 cut(s) 454
PfoI TCCNGGA 1 cut(s) 1450
PkrI GCNGC 2 cut(s) 274, 556
PleI GAGTC 1 cut(s) 1072
PpsI GAGTC 1 cut(s) 1072
PpuMI RGGWCCY 1 cut(s) 1488
Psp124BI GAGCTC 2 cut(s) 8, 356
Psp5II RGGWCCY 1 cut(s) 1488
Psp6I CCWGG 2 cut(s) 1169, 1450
PspGI CCWGG 2 cut(s) 1169, 1450
PspN4I GGNNCC 6 cut(s) 129, 653, 1396, 1468, 1482, 1490
PspOMI GGGCCC 1 cut(s) 651
PspPI GGNCC 3 cut(s) 651, 652, 1488
PspPPI RGGWCCY 1 cut(s) 1488
PsuI RGATCY 1 cut(s) 127
RseI CAYNNNNRTG 3 cut(s) 15, 1220, 1400
SacI GAGCTC 2 cut(s) 8, 356
SaqAI TTAA 4 cut(s) 524, 840, 1350, 1526
SatI GCNGC 2 cut(s) 273, 555
Sau3AI GATC 5 cut(s) 127, 157, 218, 755, 1404
Sau96I GGNCC 3 cut(s) 651, 652, 1488
ScaI AGTACT 2 cut(s) 736, 1447
SchI GAGTC 1 cut(s) 1073
ScrFI CCNGG 2 cut(s) 1171, 1452
SduI GDGCHC 3 cut(s) 8, 356, 655
SfaNI GCATC 3 cut(s) 510, 1126, 1544
SinI GGWCC 1 cut(s) 1488
SmiMI CAYNNNNRTG 3 cut(s) 15, 1220, 1400
SmlI CTYRAG 3 cut(s) 1061, 1313, 1493
SmoI CTYRAG 3 cut(s) 1061, 1313, 1493
SphI GCATGC 1 cut(s) 1378
SsiI CCGC 3 cut(s) 499, 805, 980
SspI AATATT 2 cut(s) 405, 1274
SspMI CTAG 1 cut(s) 1649
SstI GAGCTC 2 cut(s) 8, 356
StyD4I CCNGG 2 cut(s) 1169, 1450
StyI CCWWGG 4 cut(s) 488, 1179, 1398, 1632
TaaI ACNGT 5 cut(s) 729, 936, 1438, 1480, 1505
TaiI ACGT 2 cut(s) 239, 1557
TaqI TCGA 2 cut(s) 630, 1656
TaqII GACCGA 1 cut(s) 1598
TatI WGTACW 4 cut(s) 573, 734, 1234, 1445
TfiI GAWTC 1 cut(s) 454
Tru1I TTAA 4 cut(s) 524, 840, 1350, 1526
Tru9I TTAA 4 cut(s) 524, 840, 1350, 1526
TscAI CASTG 3 cut(s) 653, 1326, 1510
TseFI GTSAC 4 cut(s) 878, 1194, 1321, 1602
TseI GCWGC 2 cut(s) 272, 554
Tsp45I GTSAC 4 cut(s) 878, 1194, 1321, 1602
TspDTI ATGAA 9 cut(s) 66, 467, 496, 874, 1004, 1058, 1136, 1296, 1347
TspRI CASTG 3 cut(s) 653, 1326, 1510
VpaK11BI GGWCC 1 cut(s) 1488
XapI RAATTY 3 cut(s) 340, 800, 1174
XceI RCATGY 2 cut(s) 400, 1378
XmiI GTMKAC 2 cut(s) 724, 1205
XmnI GAANNNNTTC 1 cut(s) 73
XspI CTAG 1 cut(s) 1649
ZrmI AGTACT 2 cut(s) 736, 1447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.