Rw4G016840

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013766
Physical Location & Seq
Reverse (-)
38241968 .. 38243849
1882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G016840.1

Sequence Viewer

Length: 696 bp
ATGATCAAGGCCGATTTCATACGTCACAACCGAGGAATAGATGATGGCAAGGATCTACCTGAGGAATATCTTGGTGCACTATATGATCAGATTACCCAGAGAGATGCATTTGTCACATCAATGGCAAAGTTTACTTATCTACATAATGCTGCAGATATGAGGCAAAAGAATGTTGACGCTGTGAAGGCAATAATAGCAATCGCCGTTGAAGATGGTAATCATCTTCAAGAAGCATGGGAGCACATATTAACATGCCTTTTCCGAATTGAGCATCTGCAACTGTTGGGTGAGGGCGCACCTACTGATGCAACTTTTTTCAGCGGATCTAACGTTGAAGTGGATGACAAATCACCAAAGCGTATTGGTTTTGCTTCTCTAAAGAAAAAAGGAACTATCCAGAATCCAGCTGTGATGGCTGTTGTTCGTGGGGGTTCATATGATAGCACTAGTATTGGAGTCAACACTTCAGGACTGGTATCTCCAGAACAGATTAATAATTTTATTTTGAATTTGAATTTGCTGGATCAGATTAGGAATTTTGAGTTGAACAGTGAAGCCATAGTTTCTTTTGTGAAGTCCTTATGTAAAGTTTCCATGTCAGAATTGCAGTCTCCAACAGATCCTCATGTTTTCAGCCTCACAAAAATAGTTGAAATAGCGTTGGATTCTGCTGCAATGATATTTTTTTATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

231

Amino Acids

25.59

Weight (kDa)

5.15

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7 PF01369 1 - 34 9.9e-07 Sec7 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 321
AclI AACGTT 1 cut(s) 330
AclWI GGATC 4 cut(s) 60, 331, 531, 614
AcsI RAATTY 3 cut(s) 508, 514, 535
AcuI CTGAAG 1 cut(s) 450
AgsI TTSAA 7 cut(s) 209, 227, 335, 508, 514, 547, 653
AhlI ACTAGT 1 cut(s) 446
AluBI AGCT 1 cut(s) 407
AluI AGCT 1 cut(s) 407
Alw21I GWGCWC 2 cut(s) 79, 243
Alw26I GTCTC 1 cut(s) 615
Alw44I GTGCAC 1 cut(s) 75
AlwI GGATC 4 cut(s) 60, 331, 531, 614
AoxI GGCC 1 cut(s) 9
ApaLI GTGCAC 1 cut(s) 75
ApeKI GCWGC 2 cut(s) 149, 671
ApoI RAATTY 3 cut(s) 508, 514, 535
AseI ATTAAT 1 cut(s) 492
AspLEI GCGC 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 299, 342
AxyI CCTNAGG 1 cut(s) 60
BaeGI GKGCMC 1 cut(s) 79
Bbv12I GWGCWC 2 cut(s) 79, 243
BbvI GCAGC 2 cut(s) 136, 658
BccI CCATC 3 cut(s) 38, 206, 406
BceAI ACGGC 1 cut(s) 188
BclI TGATCA 2 cut(s) 3, 85
BcoDI GTCTC 1 cut(s) 615
BcuI ACTAGT 1 cut(s) 446
BfaI CTAG 1 cut(s) 447
BfmI CTRYAG 1 cut(s) 150
BisI GCNGC 2 cut(s) 150, 672
BlsI GCNGC 2 cut(s) 151, 673
BmsI GCATC 3 cut(s) 94, 280, 295
BpmI CTGGAG 1 cut(s) 465
BsaBI GATNNNNATC 1 cut(s) 216
BsaJI CCNNGG 1 cut(s) 31
Bse1I ACTGG 1 cut(s) 477
Bse21I CCTNAGG 1 cut(s) 60
Bse3DI GCAATG 1 cut(s) 681
Bse8I GATNNNNATC 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 31
BseGI GGATG 1 cut(s) 346
BseJI GATNNNNATC 1 cut(s) 216
BseMI GCAATG 1 cut(s) 681
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 1 cut(s) 477
BseSI GKGCMC 1 cut(s) 79
BseXI GCAGC 2 cut(s) 136, 658
BshFI GGCC 1 cut(s) 11
BsiHKAI GWGCWC 2 cut(s) 79, 243
BsmAI GTCTC 1 cut(s) 615
BsnI GGCC 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 79, 243
Bsp143I GATC 6 cut(s) 3, 52, 85, 323, 523, 619
BspACI CCGC 1 cut(s) 321
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 52
BspMAI CTGCAG 1 cut(s) 154
BspPI GGATC 4 cut(s) 60, 331, 531, 614
BsrDI GCAATG 1 cut(s) 681
BsrI ACTGG 1 cut(s) 477
BssECI CCNNGG 1 cut(s) 31
BssMI GATC 6 cut(s) 3, 52, 85, 323, 523, 619
Bst4CI ACNGT 2 cut(s) 282, 551
BstDEI CTNAG 1 cut(s) 60
BstF5I GGATG 1 cut(s) 346
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 6 cut(s) 6, 55, 88, 326, 526, 622
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 6 cut(s) 3, 52, 85, 323, 523, 619
BstMWI GCNNNNNNNGC 3 cut(s) 185, 194, 413
BstNSI RCATGY 1 cut(s) 255
BstSFI CTRYAG 1 cut(s) 150
BstSLI GKGCMC 1 cut(s) 79
BstV1I GCAGC 2 cut(s) 136, 658
BstX2I RGATCY 3 cut(s) 52, 323, 619
BstYI RGATCY 3 cut(s) 52, 323, 619
Bsu36I CCTNAGG 1 cut(s) 60
BsuRI GGCC 1 cut(s) 11
BtsCI GGATG 1 cut(s) 346
BtsIMutI CAGTG 1 cut(s) 556
CfoI GCGC 1 cut(s) 296
CseI GACGC 1 cut(s) 185
CviAII CATG 4 cut(s) 234, 252, 595, 626
CviJI RGCY 5 cut(s) 11, 407, 416, 557, 636
CviKI_1 RGCY 5 cut(s) 11, 407, 416, 557, 636
DdeI CTNAG 1 cut(s) 60
DpnI GATC 6 cut(s) 5, 54, 87, 325, 525, 621
DpnII GATC 6 cut(s) 3, 52, 85, 323, 523, 619
Eco57I CTGAAG 1 cut(s) 450
Eco81I CCTNAGG 1 cut(s) 60
EcoT22I ATGCAT 1 cut(s) 109
FaeI CATG 4 cut(s) 237, 255, 598, 629
FatI CATG 4 cut(s) 233, 251, 594, 625
FauNDI CATATG 1 cut(s) 436
FbaI TGATCA 2 cut(s) 3, 85
Fnu4HI GCNGC 2 cut(s) 150, 672
FokI GGATG 1 cut(s) 353
Fsp4HI GCNGC 2 cut(s) 150, 672
FspBI CTAG 1 cut(s) 447
GlaI GCGC 1 cut(s) 295
GluI GCNGC 2 cut(s) 150, 672
GsuI CTGGAG 1 cut(s) 465
HaeIII GGCC 1 cut(s) 11
HgaI GACGC 1 cut(s) 185
HhaI GCGC 1 cut(s) 296
Hin1II CATG 4 cut(s) 237, 255, 598, 629
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HincII GTYRAC 2 cut(s) 175, 460
HindII GTYRAC 2 cut(s) 175, 460
HinfI GANTC 3 cut(s) 400, 456, 665
HphI GGTGA 2 cut(s) 299, 342
Hpy166II GTNNAC 4 cut(s) 77, 132, 175, 460
Hpy188I TCNGA 4 cut(s) 90, 263, 528, 601
Hpy188III TCNNGA 4 cut(s) 227, 397, 468, 482
Hpy8I GTNNAC 4 cut(s) 77, 132, 175, 460
HpyAV CCTTC 1 cut(s) 178
HpyCH4III ACNGT 2 cut(s) 282, 551
HpyCH4IV ACGT 2 cut(s) 22, 330
HpyCH4V TGCA 7 cut(s) 77, 107, 152, 277, 308, 607, 674
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 194, 413
HpyF3I CTNAG 1 cut(s) 60
HpySE526I ACGT 2 cut(s) 22, 330
Hsp92II CATG 4 cut(s) 237, 255, 598, 629
HspAI GCGC 1 cut(s) 294
Ksp22I TGATCA 2 cut(s) 3, 85
Kzo9I GATC 6 cut(s) 3, 52, 85, 323, 523, 619
LmnI GCTCC 1 cut(s) 238
LpnPI CCDG 8 cut(s) 72, 110, 410, 417, 453, 458, 495, 506
Lsp1109I GCAGC 2 cut(s) 136, 658
LweI GCATC 3 cut(s) 94, 280, 295
MaeI CTAG 1 cut(s) 447
MaeII ACGT 2 cut(s) 22, 330
MaeIII GTNAC 2 cut(s) 23, 112
MalI GATC 6 cut(s) 5, 54, 87, 325, 525, 621
MboI GATC 6 cut(s) 3, 52, 85, 323, 523, 619
MboII GAAGA 2 cut(s) 215, 221
MflI RGATCY 3 cut(s) 52, 323, 619
MhlI GDGCHC 2 cut(s) 79, 243
MluCI AATT 6 cut(s) 264, 496, 508, 514, 535, 602
MlyI GAGTC 1 cut(s) 465
MmeI TCCRAC 2 cut(s) 638, 642
MnlI CCTC 6 cut(s) 26, 55, 153, 283, 633, 647
Mph1103I ATGCAT 1 cut(s) 109
MseI TTAA 2 cut(s) 248, 492
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 2 cut(s) 321, 407
MwoI GCNNNNNNNGC 3 cut(s) 185, 194, 413
NdeI CATATG 1 cut(s) 436
NdeII GATC 6 cut(s) 3, 52, 85, 323, 523, 619
NlaIII CATG 4 cut(s) 237, 255, 598, 629
NmuCI GTSAC 2 cut(s) 23, 112
NsiI ATGCAT 1 cut(s) 109
NspI RCATGY 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PfeI GAWTC 2 cut(s) 400, 665
PkrI GCNGC 2 cut(s) 151, 673
PleI GAGTC 1 cut(s) 464
PpsI GAGTC 1 cut(s) 464
PshBI ATTAAT 1 cut(s) 492
Psp1406I AACGTT 1 cut(s) 330
PstI CTGCAG 1 cut(s) 154
PsuI RGATCY 3 cut(s) 52, 323, 619
PvuII CAGCTG 1 cut(s) 407
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 2 cut(s) 248, 492
SatI GCNGC 2 cut(s) 150, 672
Sau3AI GATC 6 cut(s) 3, 52, 85, 323, 523, 619
SchI GAGTC 1 cut(s) 465
SduI GDGCHC 2 cut(s) 79, 243
SetI ASST 5 cut(s) 25, 61, 301, 333, 409
SfaNI GCATC 3 cut(s) 94, 280, 295
SfcI CTRYAG 1 cut(s) 150
SmiMI CAYNNNNRTG 1 cut(s) 119
SpeI ACTAGT 1 cut(s) 446
Sse9I AATT 6 cut(s) 264, 496, 508, 514, 535, 602
SsiI CCGC 1 cut(s) 321
SspMI CTAG 1 cut(s) 447
TaaI ACNGT 2 cut(s) 282, 551
TaiI ACGT 2 cut(s) 25, 333
TasI AATT 6 cut(s) 264, 496, 508, 514, 535, 602
TfiI GAWTC 2 cut(s) 400, 665
Tru1I TTAA 2 cut(s) 248, 492
Tru9I TTAA 2 cut(s) 248, 492
TscAI CASTG 1 cut(s) 556
TseFI GTSAC 2 cut(s) 23, 112
TseI GCWGC 2 cut(s) 149, 671
Tsp45I GTSAC 2 cut(s) 23, 112
TspDTI ATGAA 2 cut(s) 7, 423
TspRI CASTG 1 cut(s) 556
VneI GTGCAC 1 cut(s) 75
VspI ATTAAT 1 cut(s) 492
XapI RAATTY 3 cut(s) 508, 514, 535
XceI RCATGY 1 cut(s) 255
XspI CTAG 1 cut(s) 447
Zsp2I ATGCAT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.