Rw6G005940

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0006688
Physical Location & Seq
Reverse (-)
9529762 .. 9531121
1360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G005940.1

Sequence Viewer

Length: 582 bp
ATGCTGGGGTTGGATTATAATCCCCTTATAAATTCTTTTATTCAGGCTCAGTCTGAATCTGAAATAGCAGTTGCTGCTCGTCCAGTTGAGCCATTGAGGAGGAACCCACCAAGAACTGCACGTCGTGACGCGAAGCTTCAACAATTGAAGCTTCCATTAGCTGACGCGCTTTCAGCTGGGGAACGGTTTAAGGATCTTATTGCTGCTGGTGATCCATCATTGGGTGCTTTGACAATGATGGCTTTGAAAAAGGTGGTCTTTTTCTGGCAGCATCATTACTCAACCAAGTTTCAAATTTTCGATTTCGATCACAGCCTGCTTGCCGCTTACATCTGCAAGTGCATGAATGCTAGGCAGATGACGGTTGCTGCTTCTATTGTGGCTGCCAGGGATCAATGTCAGTTTGCCCATCCAAAGGATCCTAATCCGGCGAATTTTGCTCCATTTTTGGGTTTGTTGTTGAAAGCTGTGGGTCATCTTTGGGCTATTCTAGTCGTCGAGAATTCATTAAGTTTGGATGATGTCCTGAAACTCTCAAAGATCGGGATCTCGGAGTGGGCAGCCTCAAGAAGAGGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

21.36

Weight (kDa)

9.05

Isoelectric Point (pI)

35.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 18, 29
AccII CGCG 2 cut(s) 131, 167
AciI CCGC 1 cut(s) 324
AclWI GGATC 6 cut(s) 201, 206, 399, 413, 426, 554
AcsI RAATTY 4 cut(s) 31, 294, 433, 502
AdeI CACNNNGTG 1 cut(s) 125
AfiI CCNNNNNNNGG 3 cut(s) 221, 415, 449
AgsI TTSAA 5 cut(s) 140, 148, 247, 293, 463
AjiI CACGTC 1 cut(s) 122
AjnI CCWGG 1 cut(s) 386
AluBI AGCT 5 cut(s) 136, 151, 161, 176, 467
AluI AGCT 5 cut(s) 136, 151, 161, 176, 467
AlwI GGATC 6 cut(s) 201, 206, 399, 413, 426, 554
AlwNI CAGNNNCTG 1 cut(s) 74
ApeKI GCWGC 6 cut(s) 74, 203, 268, 368, 383, 560
ApoI RAATTY 4 cut(s) 31, 294, 433, 502
AspLEI GCGC 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 221
BamHI GGATCC 1 cut(s) 418
BbvI GCAGC 6 cut(s) 61, 190, 280, 355, 370, 572
BccI CCATC 3 cut(s) 223, 232, 417
BciT130I CCWGG 1 cut(s) 388
BfaI CTAG 2 cut(s) 351, 491
BisI GCNGC 7 cut(s) 75, 204, 269, 324, 369, 384, 561
BlsI GCNGC 7 cut(s) 76, 205, 270, 325, 370, 385, 562
Bme1390I CCNGG 1 cut(s) 388
BmgBI CACGTC 1 cut(s) 122
BmiI GGNNCC 2 cut(s) 104, 420
BmrFI CCNGG 1 cut(s) 388
BmsI GCATC 1 cut(s) 280
BpuEI CTTGAG 1 cut(s) 550
BsaBI GATNNNNATC 4 cut(s) 18, 306, 423, 545
BsaJI CCNNGG 1 cut(s) 387
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 415, 449
Bse1I ACTGG 1 cut(s) 83
Bse8I GATNNNNATC 4 cut(s) 18, 306, 423, 545
BseBI CCWGG 1 cut(s) 388
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 2 cut(s) 409, 523
BseJI GATNNNNATC 4 cut(s) 18, 306, 423, 545
BseLI CCNNNNNNNGG 3 cut(s) 221, 415, 449
BseMII CTCAG 1 cut(s) 62
BseNI ACTGG 1 cut(s) 83
BseRI GAGGAG 1 cut(s) 112
BseXI GCAGC 6 cut(s) 61, 190, 280, 355, 370, 572
BseYI CCCAGC 2 cut(s) 4, 176
BsgI GTGCAG 1 cut(s) 102
Bsh1236I CGCG 2 cut(s) 131, 167
BsiSI CCGG 1 cut(s) 428
BslI CCNNNNNNNGG 3 cut(s) 221, 415, 449
BsmI GAATGC 1 cut(s) 352
Bsp143I GATC 7 cut(s) 193, 211, 307, 391, 418, 540, 546
BspACI CCGC 1 cut(s) 324
BspCNI CTCAG 1 cut(s) 61
BspFNI CGCG 2 cut(s) 131, 167
BspLI GGNNCC 2 cut(s) 104, 420
BspPI GGATC 6 cut(s) 201, 206, 399, 413, 426, 554
BsrI ACTGG 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 7 cut(s) 193, 211, 307, 391, 418, 540, 546
Bst2UI CCWGG 1 cut(s) 388
Bst4CI ACNGT 2 cut(s) 186, 364
Bst6I CTCTTC 1 cut(s) 565
BstAPI GCANNNNNTGC 1 cut(s) 74
BstC8I GCNNGC 2 cut(s) 317, 321
BstDEI CTNAG 1 cut(s) 48
BstF5I GGATG 2 cut(s) 409, 523
BstFNI CGCG 2 cut(s) 131, 167
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 7 cut(s) 196, 214, 310, 394, 421, 543, 549
BstMBI GATC 7 cut(s) 193, 211, 307, 391, 418, 540, 546
BstMWI GCNNNNNNNGC 3 cut(s) 74, 173, 437
BstNI CCWGG 1 cut(s) 388
BstSCI CCNGG 1 cut(s) 386
BstUI CGCG 2 cut(s) 131, 167
BstV1I GCAGC 6 cut(s) 61, 190, 280, 355, 370, 572
BstX2I RGATCY 3 cut(s) 193, 418, 546
BstYI RGATCY 3 cut(s) 193, 418, 546
BtrI CACGTC 1 cut(s) 122
BtsCI GGATG 2 cut(s) 409, 523
Cac8I GCNNGC 2 cut(s) 317, 321
CaiI CAGNNNCTG 1 cut(s) 74
CfoI GCGC 1 cut(s) 169
CseI GACGC 2 cut(s) 137, 173
CviAII CATG 1 cut(s) 343
DdeI CTNAG 1 cut(s) 48
DpnI GATC 7 cut(s) 195, 213, 309, 393, 420, 542, 548
DpnII GATC 7 cut(s) 193, 211, 307, 391, 418, 540, 546
DraIII CACNNNGTG 1 cut(s) 125
Eam1104I CTCTTC 1 cut(s) 565
EarI CTCTTC 1 cut(s) 565
EcoRI GAATTC 1 cut(s) 502
EcoRII CCWGG 1 cut(s) 386
FaeI CATG 1 cut(s) 346
FaiI YATR 3 cut(s) 18, 29, 344
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FatI CATG 1 cut(s) 342
Fnu4HI GCNGC 7 cut(s) 75, 204, 269, 324, 369, 384, 561
FokI GGATG 2 cut(s) 396, 530
Fsp4HI GCNGC 7 cut(s) 75, 204, 269, 324, 369, 384, 561
FspBI CTAG 2 cut(s) 351, 491
GlaI GCGC 1 cut(s) 168
GluI GCNGC 7 cut(s) 75, 204, 269, 324, 369, 384, 561
GsaI CCCAGC 2 cut(s) 8, 180
HapII CCGG 1 cut(s) 428
HgaI GACGC 2 cut(s) 137, 173
HhaI GCGC 1 cut(s) 169
Hin1II CATG 1 cut(s) 346
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HindIII AAGCTT 2 cut(s) 134, 149
HinfI GANTC 1 cut(s) 56
HpaII CCGG 1 cut(s) 428
HphI GGTGA 1 cut(s) 221
Hpy188I TCNGA 3 cut(s) 55, 61, 553
Hpy188III TCNNGA 5 cut(s) 125, 499, 526, 544, 567
Hpy99I CGWCG 2 cut(s) 126, 500
HpyCH4III ACNGT 2 cut(s) 186, 364
HpyCH4IV ACGT 1 cut(s) 121
HpyCH4V TGCA 3 cut(s) 119, 336, 342
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 173, 437
HpyF3I CTNAG 1 cut(s) 48
HpySE526I ACGT 1 cut(s) 121
Hsp92II CATG 1 cut(s) 346
HspAI GCGC 1 cut(s) 167
Kzo9I GATC 7 cut(s) 193, 211, 307, 391, 418, 540, 546
LmnI GCTCC 1 cut(s) 445
Lsp1109I GCAGC 6 cut(s) 61, 190, 280, 355, 370, 572
LweI GCATC 1 cut(s) 280
MaeI CTAG 2 cut(s) 351, 491
MaeII ACGT 1 cut(s) 121
MaeIII GTNAC 1 cut(s) 125
MalI GATC 7 cut(s) 195, 213, 309, 393, 420, 542, 548
MboI GATC 7 cut(s) 193, 211, 307, 391, 418, 540, 546
MboII GAAGA 1 cut(s) 582
MfeI CAATTG 1 cut(s) 143
MflI RGATCY 3 cut(s) 193, 418, 546
MluCI AATT 5 cut(s) 31, 143, 294, 433, 502
MnlI CCTC 4 cut(s) 90, 93, 566, 574
MseI TTAA 2 cut(s) 189, 509
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 1 cut(s) 428
MspR9I CCNGG 1 cut(s) 388
MunI CAATTG 1 cut(s) 143
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 1 cut(s) 388
MvnI CGCG 2 cut(s) 131, 167
MwoI GCNNNNNNNGC 3 cut(s) 74, 173, 437
NdeII GATC 7 cut(s) 193, 211, 307, 391, 418, 540, 546
NlaIII CATG 1 cut(s) 346
NlaIV GGNNCC 2 cut(s) 104, 420
NmuCI GTSAC 1 cut(s) 125
PctI GAATGC 1 cut(s) 352
PfeI GAWTC 1 cut(s) 56
PkrI GCNGC 7 cut(s) 76, 205, 270, 325, 370, 385, 562
PsiI TTATAA 2 cut(s) 18, 29
Psp6I CCWGG 1 cut(s) 386
PspFI CCCAGC 2 cut(s) 4, 176
PspGI CCWGG 1 cut(s) 386
PspN4I GGNNCC 2 cut(s) 104, 420
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 3 cut(s) 193, 418, 546
PvuII CAGCTG 1 cut(s) 176
SaqAI TTAA 2 cut(s) 189, 509
SatI GCNGC 7 cut(s) 75, 204, 269, 324, 369, 384, 561
Sau3AI GATC 7 cut(s) 193, 211, 307, 391, 418, 540, 546
ScrFI CCNGG 1 cut(s) 388
SetI ASST 8 cut(s) 124, 138, 153, 163, 178, 255, 469, 577
SfaNI GCATC 1 cut(s) 280
SmlI CTYRAG 1 cut(s) 565
SmoI CTYRAG 1 cut(s) 565
Sse9I AATT 5 cut(s) 31, 143, 294, 433, 502
SsiI CCGC 1 cut(s) 324
SspMI CTAG 2 cut(s) 351, 491
StyD4I CCNGG 1 cut(s) 386
TaaI ACNGT 2 cut(s) 186, 364
TaiI ACGT 1 cut(s) 124
TaqI TCGA 3 cut(s) 300, 306, 498
TasI AATT 5 cut(s) 31, 143, 294, 433, 502
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 1 cut(s) 56
Tru1I TTAA 2 cut(s) 189, 509
Tru9I TTAA 2 cut(s) 189, 509
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 6 cut(s) 74, 203, 268, 368, 383, 560
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 2 cut(s) 359, 495
XapI RAATTY 4 cut(s) 31, 294, 433, 502
XspI CTAG 2 cut(s) 351, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.