Rw7G008550

Lysosomal alpha-mannosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0013740
Physical Location & Seq
Reverse (-)
7795920 .. 7798263
2344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G008550.1

Sequence Viewer

Length: 354 bp
ATGGCGGTTGTGGCTTGCTTTAGGCTTGTATTAGTATTCACTGTGTTTCTACATGCGGCGGAGTCCAAGTACGTACAGTACAATACAACATCGGTCCTTGTTCCTGGAAAGCTGAACGTTCATTTGGTCGCTCACACCCACGACGACGTCGGATGGCTGAAGACCATGGACCAGTACTACGTTGGCTCCAATAATTCCTTCCAGGAAGCTTGTGTTCAGAACGTGCTGGATTCGTTGGTACTGGCATTGTTGGCCGATAAGAACCGCAAGTTTGTTTACACTGAGCAGATTCGAGTCTATAGAAAAGACTGGGACCTGGGAGTGAATCAACCTGTTGCTGGAAATTATCATCCG

Protein Analysis

118

Amino Acids

13.42

Weight (kDa)

6.95

Isoelectric Point (pI)

21.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_38N PF01074 39 - 102 1.9e-12 Glycosyl hydrolases family 38 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AciI CCGC 4 cut(s) 5, 56, 59, 265
AclI AACGTT 1 cut(s) 117
AcoI YGGCCR 1 cut(s) 252
AcuI CTGAAG 1 cut(s) 179
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 5 cut(s) 71, 75, 80, 176, 240
AfiI CCNNNNNNNGG 1 cut(s) 338
AjnI CCWGG 3 cut(s) 103, 201, 315
AjuI GAANNNNNNNTTGG 4 cut(s) 107, 139, 182, 214
AluBI AGCT 2 cut(s) 112, 209
AluI AGCT 2 cut(s) 112, 209
AoxI GGCC 1 cut(s) 252
AspS9I GGNCC 3 cut(s) 94, 169, 313
AvaII GGWCC 3 cut(s) 94, 169, 313
BbsI GAAGAC 1 cut(s) 167
BccI CCATC 1 cut(s) 147
BciT130I CCWGG 3 cut(s) 105, 203, 317
BfmI CTRYAG 1 cut(s) 298
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
BmcAI AGTACT 1 cut(s) 176
Bme1390I CCNGG 3 cut(s) 105, 203, 317
Bme18I GGWCC 3 cut(s) 94, 169, 313
BmgT120I GGNCC 3 cut(s) 94, 169, 313
BmiI GGNNCC 2 cut(s) 187, 314
BmrFI CCNGG 3 cut(s) 105, 203, 317
BmrI ACTGGG 1 cut(s) 319
BmuI ACTGGG 1 cut(s) 319
BpiI GAAGAC 1 cut(s) 167
BsaAI YACGTR 1 cut(s) 73
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 2 cut(s) 165, 316
BsaXI ACNNNNNCTCC 4 cut(s) 53, 83, 170, 200
Bsc4I CCNNNNNNNGG 1 cut(s) 338
Bse1I ACTGG 3 cut(s) 172, 246, 314
BseBI CCWGG 3 cut(s) 105, 203, 317
BseDI CCNNGG 2 cut(s) 165, 316
BseGI GGATG 2 cut(s) 158, 349
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 1 cut(s) 273
BseNI ACTGG 3 cut(s) 172, 246, 314
BshFI GGCC 1 cut(s) 254
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 326
BsnI GGCC 1 cut(s) 254
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 4 cut(s) 5, 56, 59, 265
BspANI GGCC 1 cut(s) 254
BspCNI CTCAG 1 cut(s) 274
BspLI GGNNCC 2 cut(s) 187, 314
BsrI ACTGG 3 cut(s) 172, 246, 314
BssECI CCNNGG 2 cut(s) 165, 316
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 3 cut(s) 105, 203, 317
Bst4CI ACNGT 2 cut(s) 43, 78
BstACI GRCGYC 1 cut(s) 147
BstBAI YACGTR 1 cut(s) 73
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 1 cut(s) 282
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 2 cut(s) 158, 349
BstMWI GCNNNNNNNGC 2 cut(s) 11, 251
BstNI CCWGG 3 cut(s) 105, 203, 317
BstNSI RCATGY 1 cut(s) 56
BstSCI CCNGG 3 cut(s) 103, 201, 315
BstSFI CTRYAG 1 cut(s) 298
BstSNI TACGTA 1 cut(s) 73
BstV2I GAAGAC 1 cut(s) 167
BsuRI GGCC 1 cut(s) 254
BtgI CCRYGG 1 cut(s) 165
BtsCI GGATG 2 cut(s) 158, 349
BtsIMutI CAGTG 2 cut(s) 39, 279
Cac8I GCNNGC 1 cut(s) 16
Cfr13I GGNCC 3 cut(s) 94, 169, 313
Csp6I GTAC 5 cut(s) 70, 74, 79, 175, 239
CviAII CATG 2 cut(s) 53, 166
CviJI RGCY 7 cut(s) 14, 25, 112, 157, 186, 209, 254
CviKI_1 RGCY 7 cut(s) 14, 25, 112, 157, 186, 209, 254
CviQI GTAC 5 cut(s) 70, 74, 79, 175, 239
DdeI CTNAG 1 cut(s) 282
EaeI YGGCCR 1 cut(s) 252
EciI GGCGGA 1 cut(s) 74
Eco105I TACGTA 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 165
Eco47I GGWCC 3 cut(s) 94, 169, 313
Eco57I CTGAAG 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 313
EcoRII CCWGG 3 cut(s) 103, 201, 315
EcoT14I CCWWGG 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 2 cut(s) 56, 169
FaiI YATR 3 cut(s) 54, 167, 300
FaqI GGGAC 1 cut(s) 326
FatI CATG 2 cut(s) 52, 165
Fnu4HI GCNGC 1 cut(s) 57
FokI GGATG 2 cut(s) 165, 336
Fsp4HI GCNGC 1 cut(s) 57
GluI GCNGC 1 cut(s) 57
HaeIII GGCC 1 cut(s) 254
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 2 cut(s) 56, 169
HindIII AAGCTT 1 cut(s) 207
HinfI GANTC 5 cut(s) 62, 230, 289, 294, 325
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 2 cut(s) 152, 219
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 3 cut(s) 146, 149, 152
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 2 cut(s) 43, 78
HpyCH4IV ACGT 5 cut(s) 72, 117, 147, 180, 222
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 251
HpyF3I CTNAG 1 cut(s) 282
HpySE526I ACGT 5 cut(s) 72, 117, 147, 180, 222
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 2 cut(s) 56, 169
LmnI GCTCC 1 cut(s) 191
MaeII ACGT 5 cut(s) 72, 117, 147, 180, 222
MboII GAAGA 1 cut(s) 172
MluCI AATT 2 cut(s) 193, 343
MlyI GAGTC 2 cut(s) 71, 303
MmeI TCCRAC 1 cut(s) 130
MspR9I CCNGG 3 cut(s) 105, 203, 317
MvaI CCWGG 3 cut(s) 105, 203, 317
MwoI GCNNNNNNNGC 2 cut(s) 11, 251
NcoI CCATGG 1 cut(s) 165
NlaIII CATG 2 cut(s) 56, 169
NlaIV GGNNCC 2 cut(s) 187, 314
NspI RCATGY 1 cut(s) 56
PfeI GAWTC 3 cut(s) 230, 289, 325
PflFI GACNNNGTC 1 cut(s) 146
PfoI TCCNGGA 2 cut(s) 103, 201
PkrI GCNGC 1 cut(s) 58
PleI GAGTC 2 cut(s) 70, 302
PpsI GAGTC 2 cut(s) 70, 302
Ppu21I YACGTR 1 cut(s) 73
PpuMI RGGWCCY 1 cut(s) 313
Psp1406I AACGTT 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 313
Psp6I CCWGG 3 cut(s) 103, 201, 315
PspGI CCWGG 3 cut(s) 103, 201, 315
PspN4I GGNNCC 2 cut(s) 187, 314
PspPI GGNCC 3 cut(s) 94, 169, 313
PspPPI RGGWCCY 1 cut(s) 313
PsyI GACNNNGTC 1 cut(s) 146
RsaI GTAC 5 cut(s) 71, 75, 80, 176, 240
RsaNI GTAC 5 cut(s) 70, 74, 79, 175, 239
SatI GCNGC 1 cut(s) 57
Sau96I GGNCC 3 cut(s) 94, 169, 313
ScaI AGTACT 1 cut(s) 176
SchI GAGTC 2 cut(s) 71, 303
ScrFI CCNGG 3 cut(s) 105, 203, 317
SetI ASST 9 cut(s) 75, 114, 120, 150, 183, 211, 225, 318, 334
SfcI CTRYAG 1 cut(s) 298
SinI GGWCC 3 cut(s) 94, 169, 313
SnaBI TACGTA 1 cut(s) 73
Sse9I AATT 2 cut(s) 193, 343
SsiI CCGC 4 cut(s) 5, 56, 59, 265
StyD4I CCNGG 3 cut(s) 103, 201, 315
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 43, 78
TaiI ACGT 5 cut(s) 75, 120, 150, 183, 225
TaqI TCGA 1 cut(s) 292
TaqII GACCGA 1 cut(s) 82
TasI AATT 2 cut(s) 193, 343
TatI WGTACW 2 cut(s) 78, 174
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 3 cut(s) 230, 289, 325
TscAI CASTG 2 cut(s) 46, 286
TspDTI ATGAA 1 cut(s) 110
TspRI CASTG 2 cut(s) 46, 286
Tth111I GACNNNGTC 1 cut(s) 146
VpaK11BI GGWCC 3 cut(s) 94, 169, 313
XceI RCATGY 1 cut(s) 56
XcmI CCANNNNNNNNNTGG 1 cut(s) 179
ZraI GACGTC 1 cut(s) 148
ZrmI AGTACT 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.