Rw7G020850

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011430
Physical Location & Seq
Forward (+)
23925349 .. 23928693
3345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G020850.1

Sequence Viewer

Length: 1119 bp
ATGGAGCTTCCGCTCATATCGCTAGCCGCTACTACTAGTACTTCCACGGCGTCGTTTGGCAGCAAGCTCGGGCTGTGCGATTTTCCGGTGACGAGAAGGAGGCGGACGGGTTTGTTCGACGGAGGTAGGGTTTCTGTGAGGCAGGTGGTCAGGGCTTCGGCGGAGCAGAGGAGTAGCGACGGTATTAATGGAAGTGAGGCCGAGACTGGAGGACGGCGGTTCACCAGTCCTGCCATGGAGGTCACCACATTGAATCCGAGCTTCAACAACGAAGCTGAGTATCCTGTTTGGGAGAAGATTGGTGCTGTTGTTAGACTTAGTTATGGAATTGGTATATATGGTGCAATGGCTTTAGCGGGTAGATTTATATGCTCAATGATGGGTATCGATATCGTGGGAGGTTTCGAGTTAAATTTAGATGCAATTGTACAAGGCCTTGGATATGCAGCTCCACCAATCATGGCGCTTCTTTTTATACTCGATGATGAAGTTGTAAAGTTATCACCTCATGCACGTGCAATTAGAGACGTAGAGGATGAGGAGCTGCGCAGTTTCTTTTATGGAATGTCACCGTTGCAGTTCATACTAATTGTTACTGCAAGCTCTTTGGGAGAGGAACTTTTCTACAGGGCTGCTGTTCAGGGATCATTAGCCGAGATATTTTTGAAGGGAACAGATCTGGTGACAGACATTCGAGGAATGGCATCTCTGTCTGGGGTTCTACCCCCATTTGTCCCATTTGCTCAGGCATTTGCAGCAGTGATCACAGCTGCTCTTACAGGTTCTTTGTACTATGTGGCTGCCTCTCCAAAAGATCCTGCCTATGTAGTTGCACCAGTTTTACAATCTCGCTCTGGTCGTGAAGATCTGAAAAAGCTGTTTGCAGCTTGGTATCAGAGAAGGCAAATGAAGAAGATCTACTCTCCCCTTCTTGAAGGACTTTTGGCCCTCTATCTCGGTTTTGAGTGGAACCAGACGAATAATCTTCTTGCACCTATGATCACCCACGGCATATATTCTGCTGTGATATTGGGTCATGGCCTTTGGAAGATACATGATCATCGGAGACGACTACATCAGAGAATCGAGCAGCTTAAAGAAGAAAGTAGAGTAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

372

Amino Acids

40.84

Weight (kDa)

7.03

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 191 - 341 7.1e-06 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 133
Acc16I TGCGCA 1 cut(s) 548
Acc36I ACCTGC 1 cut(s) 133
AccBSI CCGCTC 1 cut(s) 13
AciI CCGC 6 cut(s) 11, 27, 103, 161, 217, 356
AclWI GGATC 2 cut(s) 652, 809
AcsI RAATTY 1 cut(s) 412
AcvI CACGTG 1 cut(s) 515
AcyI GRCGYC 1 cut(s) 50
AfaI GTAC 3 cut(s) 40, 429, 791
AgsI TTSAA 4 cut(s) 253, 265, 667, 935
AhlI ACTAGT 1 cut(s) 35
Alw26I GTCTC 3 cut(s) 197, 519, 1060
AlwI GGATC 2 cut(s) 652, 809
Ama87I CYCGRG 1 cut(s) 68
AoxI GGCC 4 cut(s) 198, 433, 945, 1039
ApeKI GCWGC 9 cut(s) 60, 446, 544, 632, 755, 770, 800, 884, 1090
ApoI RAATTY 1 cut(s) 412
AseI ATTAAT 1 cut(s) 186
AspLEI GCGC 2 cut(s) 466, 549
AspS9I GGNCC 1 cut(s) 946
AsuHPI GGTGA 7 cut(s) 100, 214, 235, 495, 561, 694, 994
AsuNHI GCTAGC 1 cut(s) 22
AvaI CYCGRG 1 cut(s) 68
BarI GAAGNNNNNNTAC 2 cut(s) 902, 934
BbrPI CACGTG 1 cut(s) 515
BbvI GCAGC 9 cut(s) 72, 458, 531, 619, 757, 767, 787, 896, 1102
BccI CCATC 1 cut(s) 373
BceAI ACGGC 3 cut(s) 63, 230, 1024
BcgI CGANNNNNNTGC 2 cut(s) 49, 83
BciVI GTATCC 1 cut(s) 291
BclI TGATCA 3 cut(s) 762, 999, 1057
BcoDI GTCTC 3 cut(s) 197, 519, 1060
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 2 cut(s) 23, 36
BfmI CTRYAG 1 cut(s) 625
BfoI RGCGCY 1 cut(s) 467
BfuAI ACCTGC 1 cut(s) 133
BfuI GTATCC 1 cut(s) 291
BglII AGATCT 3 cut(s) 676, 865, 915
BmcAI AGTACT 1 cut(s) 40
BmeT110I CYCGRG 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 946
BmiI GGNNCC 1 cut(s) 971
BmsI GCATC 2 cut(s) 409, 713
BmtI GCTAGC 1 cut(s) 26
BpmI CTGGAG 1 cut(s) 228
Bpu10I CCTNAGC 1 cut(s) 744
Bsa29I ATCGAT 1 cut(s) 387
BsaAI YACGTR 1 cut(s) 515
BsaBI GATNNNNATC 1 cut(s) 383
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 4 cut(s) 45, 234, 436, 1006
BsaWI WCCGGW 1 cut(s) 85
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bse1I ACTGG 3 cut(s) 211, 225, 836
Bse3DI GCAATG 1 cut(s) 351
Bse8I GATNNNNATC 1 cut(s) 383
BseCI ATCGAT 1 cut(s) 387
BseDI CCNNGG 4 cut(s) 45, 234, 436, 1006
BseGI GGATG 1 cut(s) 541
BseJI GATNNNNATC 1 cut(s) 383
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 2 cut(s) 267, 758
BseNI ACTGG 3 cut(s) 211, 225, 836
BseRI GAGGAG 2 cut(s) 184, 554
BseXI GCAGC 9 cut(s) 72, 458, 531, 619, 757, 767, 787, 896, 1102
BshFI GGCC 4 cut(s) 200, 435, 947, 1041
BshVI ATCGAT 1 cut(s) 387
BsiHKCI CYCGRG 1 cut(s) 68
BsiSI CCGG 1 cut(s) 86
BslFI GGGAC 1 cut(s) 719
BsmAI GTCTC 3 cut(s) 197, 519, 1060
BsmBI CGTCTC 2 cut(s) 519, 1060
BsmFI GGGAC 1 cut(s) 719
BsnI GGCC 4 cut(s) 200, 435, 947, 1041
BsoBI CYCGRG 1 cut(s) 68
Bsp1407I TGTACA 1 cut(s) 427
Bsp143I GATC 8 cut(s) 644, 676, 762, 814, 865, 915, 999, 1057
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 6 cut(s) 11, 27, 103, 161, 217, 356
BspANI GGCC 4 cut(s) 200, 435, 947, 1041
BspCNI CTCAG 2 cut(s) 268, 757
BspDI ATCGAT 1 cut(s) 387
BspLI GGNNCC 1 cut(s) 971
BspMI ACCTGC 1 cut(s) 133
BspOI GCTAGC 1 cut(s) 26
BspPI GGATC 2 cut(s) 652, 809
BsrBI CCGCTC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 351
BsrGI TGTACA 1 cut(s) 427
BsrI ACTGG 3 cut(s) 211, 225, 836
BssECI CCNNGG 4 cut(s) 45, 234, 436, 1006
BssMI GATC 8 cut(s) 644, 676, 762, 814, 865, 915, 999, 1057
BssNI GRCGYC 1 cut(s) 50
BssT1I CCWWGG 2 cut(s) 234, 436
Bst4CI ACNGT 2 cut(s) 182, 573
BstACI GRCGYC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 427
BstBAI YACGTR 1 cut(s) 515
BstC8I GCNNGC 3 cut(s) 24, 65, 601
BstDEI CTNAG 3 cut(s) 276, 317, 744
BstDSI CCRYGG 3 cut(s) 45, 234, 1006
BstEII GGTNACC 1 cut(s) 241
BstF5I GGATG 1 cut(s) 541
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 2 cut(s) 466, 549
BstKTI GATC 8 cut(s) 647, 679, 765, 817, 868, 918, 1002, 1060
BstMAI GTCTC 3 cut(s) 197, 519, 1060
BstMBI GATC 8 cut(s) 644, 676, 762, 814, 865, 915, 999, 1057
BstMWI GCNNNNNNNGC 2 cut(s) 19, 755
BstPI GGTNACC 1 cut(s) 241
BstSFI CTRYAG 1 cut(s) 625
BstV1I GCAGC 9 cut(s) 72, 458, 531, 619, 757, 767, 787, 896, 1102
BstX2I RGATCY 4 cut(s) 676, 814, 865, 915
BstYI RGATCY 4 cut(s) 676, 814, 865, 915
Bsu15I ATCGAT 1 cut(s) 387
BsuI GTATCC 1 cut(s) 291
BsuRI GGCC 4 cut(s) 200, 435, 947, 1041
BsuTUI ATCGAT 1 cut(s) 387
BtgI CCRYGG 3 cut(s) 45, 234, 1006
BtsCI GGATG 1 cut(s) 541
BtsI GCAGTG 1 cut(s) 765
BtsIMutI CAGTG 1 cut(s) 765
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 3 cut(s) 24, 65, 601
CfoI GCGC 2 cut(s) 466, 549
Cfr13I GGNCC 1 cut(s) 946
ClaI ATCGAT 1 cut(s) 387
CseI GACGC 1 cut(s) 39
Csp6I GTAC 3 cut(s) 39, 428, 790
CviAII CATG 5 cut(s) 235, 460, 509, 1037, 1055
CviQI GTAC 3 cut(s) 39, 428, 790
DdeI CTNAG 3 cut(s) 276, 317, 744
DpnI GATC 8 cut(s) 646, 678, 764, 816, 867, 917, 1001, 1059
DpnII GATC 8 cut(s) 644, 676, 762, 814, 865, 915, 999, 1057
EciI GGCGGA 2 cut(s) 118, 176
Eco130I CCWWGG 2 cut(s) 234, 436
Eco147I AGGCCT 1 cut(s) 435
Eco32I GATATC 1 cut(s) 391
Eco72I CACGTG 1 cut(s) 515
Eco88I CYCGRG 1 cut(s) 68
Eco91I GGTNACC 1 cut(s) 241
EcoO65I GGTNACC 1 cut(s) 241
EcoRV GATATC 1 cut(s) 391
EcoT14I CCWWGG 2 cut(s) 234, 436
ErhI CCWWGG 2 cut(s) 234, 436
Esp3I CGTCTC 2 cut(s) 519, 1060
FaeI CATG 5 cut(s) 238, 463, 512, 1040, 1058
FaqI GGGAC 1 cut(s) 719
FatI CATG 5 cut(s) 234, 459, 508, 1036, 1054
FauI CCCGC 1 cut(s) 349
FbaI TGATCA 3 cut(s) 762, 999, 1057
FokI GGATG 1 cut(s) 548
FspBI CTAG 2 cut(s) 23, 36
FspI TGCGCA 1 cut(s) 548
GlaI GCGC 2 cut(s) 465, 548
GsuI CTGGAG 1 cut(s) 228
HaeII RGCGCY 1 cut(s) 467
HaeIII GGCC 4 cut(s) 200, 435, 947, 1041
HapII CCGG 1 cut(s) 86
HgaI GACGC 1 cut(s) 39
HhaI GCGC 2 cut(s) 466, 549
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 5 cut(s) 238, 463, 512, 1040, 1058
Hin6I GCGC 2 cut(s) 464, 547
HinP1I GCGC 2 cut(s) 464, 547
HinfI GANTC 2 cut(s) 253, 1083
HpaII CCGG 1 cut(s) 86
HphI GGTGA 7 cut(s) 100, 214, 235, 495, 561, 694, 994
Hpy166II GTNNAC 1 cut(s) 222
Hpy188I TCNGA 5 cut(s) 258, 870, 897, 1065, 1080
Hpy188III TCNNGA 2 cut(s) 860, 932
Hpy8I GTNNAC 1 cut(s) 222
Hpy99I CGWCG 3 cut(s) 55, 122, 182
HpyAV CCTTC 5 cut(s) 90, 661, 894, 929, 938
HpyCH4III ACNGT 2 cut(s) 182, 573
HpyCH4IV ACGT 2 cut(s) 514, 528
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 755
HpyF3I CTNAG 3 cut(s) 276, 317, 744
HpySE526I ACGT 2 cut(s) 514, 528
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 5 cut(s) 238, 463, 512, 1040, 1058
HspAI GCGC 2 cut(s) 464, 547
Ksp22I TGATCA 3 cut(s) 762, 999, 1057
Kzo9I GATC 8 cut(s) 644, 676, 762, 814, 865, 915, 999, 1057
LmnI GCTCC 4 cut(s) 4, 163, 454, 541
Lsp1109I GCAGC 9 cut(s) 72, 458, 531, 619, 757, 767, 787, 896, 1102
LweI GCATC 2 cut(s) 409, 713
MaeI CTAG 2 cut(s) 23, 36
MaeII ACGT 2 cut(s) 514, 528
MaeIII GTNAC 5 cut(s) 88, 241, 567, 592, 682
MalI GATC 8 cut(s) 646, 678, 764, 816, 867, 917, 1001, 1059
MbiI CCGCTC 1 cut(s) 13
MboI GATC 8 cut(s) 644, 676, 762, 814, 865, 915, 999, 1057
MboII GAAGA 7 cut(s) 307, 875, 922, 925, 977, 1060, 1112
MfeI CAATTG 1 cut(s) 423
MflI RGATCY 4 cut(s) 676, 814, 865, 915
MluCI AATT 5 cut(s) 327, 412, 423, 519, 588
MseI TTAA 3 cut(s) 186, 410, 1095
MslI CAYNNNNRTG 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 770
MspI CCGG 1 cut(s) 86
MunI CAATTG 1 cut(s) 423
MwoI GCNNNNNNNGC 2 cut(s) 19, 755
NcoI CCATGG 1 cut(s) 234
NdeII GATC 8 cut(s) 644, 676, 762, 814, 865, 915, 999, 1057
NheI GCTAGC 1 cut(s) 22
NlaIII CATG 5 cut(s) 238, 463, 512, 1040, 1058
NlaIV GGNNCC 1 cut(s) 971
NmeAIII GCCGAG 2 cut(s) 226, 679
NmuCI GTSAC 4 cut(s) 88, 241, 567, 682
NsbI TGCGCA 1 cut(s) 548
PaqCI CACCTGC 1 cut(s) 133
PceI AGGCCT 1 cut(s) 435
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 856
PfeI GAWTC 2 cut(s) 253, 1083
PmaCI CACGTG 1 cut(s) 515
PmlI CACGTG 1 cut(s) 515
Ppu21I YACGTR 1 cut(s) 515
PshBI ATTAAT 1 cut(s) 186
PspCI CACGTG 1 cut(s) 515
PspEI GGTNACC 1 cut(s) 241
PspN4I GGNNCC 1 cut(s) 971
PspPI GGNCC 1 cut(s) 946
PsuI RGATCY 4 cut(s) 676, 814, 865, 915
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 3 cut(s) 40, 429, 791
RsaNI GTAC 3 cut(s) 39, 428, 790
RseI CAYNNNNRTG 1 cut(s) 513
SaqAI TTAA 3 cut(s) 186, 410, 1095
Sau3AI GATC 8 cut(s) 644, 676, 762, 814, 865, 915, 999, 1057
Sau96I GGNCC 1 cut(s) 946
ScaI AGTACT 1 cut(s) 40
SfaNI GCATC 2 cut(s) 409, 713
SfcI CTRYAG 1 cut(s) 625
SmiMI CAYNNNNRTG 1 cut(s) 513
SpeI ACTAGT 1 cut(s) 35
Sse9I AATT 5 cut(s) 327, 412, 423, 519, 588
SseBI AGGCCT 1 cut(s) 435
SsiI CCGC 6 cut(s) 11, 27, 103, 161, 217, 356
SspMI CTAG 2 cut(s) 23, 36
StuI AGGCCT 1 cut(s) 435
StyI CCWWGG 2 cut(s) 234, 436
TaaI ACNGT 2 cut(s) 182, 573
TaiI ACGT 2 cut(s) 517, 531
TaqI TCGA 6 cut(s) 117, 387, 405, 480, 694, 1086
TasI AATT 5 cut(s) 327, 412, 423, 519, 588
TatI WGTACW 3 cut(s) 38, 427, 789
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 2 cut(s) 253, 1083
Tru1I TTAA 3 cut(s) 186, 410, 1095
Tru9I TTAA 3 cut(s) 186, 410, 1095
TscAI CASTG 1 cut(s) 765
TseFI GTSAC 4 cut(s) 88, 241, 567, 682
TseI GCWGC 9 cut(s) 60, 446, 544, 632, 755, 770, 800, 884, 1090
Tsp45I GTSAC 4 cut(s) 88, 241, 567, 682
TspDTI ATGAA 3 cut(s) 501, 571, 923
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 1 cut(s) 765
VspI ATTAAT 1 cut(s) 186
XapI RAATTY 1 cut(s) 412
XcmI CCANNNNNNNNNTGG 1 cut(s) 232
XspI CTAG 2 cut(s) 23, 36
ZrmI AGTACT 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.