AT1G03700

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008048
Physical Location & Seq
Forward (+)
920950 .. 922098
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G03700.1

Sequence Viewer

Length: 495 bp
ATGGTGAAGTTAACGCAGAGATTGGGTGGGTTGGTACTGCGATTTGCGGCATTTTGTGCCGCGTTAGGAGCGGTTATCGCAATGATCACCAGCCGTGAAAGATCTTCCTTCTTTGTCATCTCCCTAGTGGCTAAGTATAGCGATTTGGCTGCGTTTAAGTACTTTGTGATCGCAAATGCGATTGTGACCGTTTATAGCTTTCTCGTTCTGTTTCTTCCAAAGGAGAGTTTATTATGGAAGTTCGTAGTTGTGTTGGACCTGATGGTGACAATGCTGCTGACGTCAAGCTTATCAGCGGCAGTGGCAGTGGCCCAAGTGGGAAAAAGAGGAAACGCAAACGCAGGATGGTTGCCAATATGCGGTCAAGTTCCGAGATTTTGCGATCAGATAACCGGAGCTCTAATCGCCGGTTTAGTTGCATTGGTTCTCTACGTTTTCTTGCTCATCTTCTCTATCCACCATGTTGTTGATCCTTTTCTTCTTCGAAAATCATGA

Protein Analysis

164

Amino Acids

17.86

Weight (kDa)

9.82

Isoelectric Point (pI)

21.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 7 - 143 6.7e-37 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 284
AccBSI CCGCTC 1 cut(s) 71
AccII CGCG 1 cut(s) 62
AciI CCGC 5 cut(s) 47, 60, 71, 296, 360
AclWI GGATC 1 cut(s) 464
AcyI GRCGYC 1 cut(s) 281
AfaI GTAC 2 cut(s) 36, 161
AfiI CCNNNNNNNGG 1 cut(s) 359
AluBI AGCT 3 cut(s) 198, 288, 398
AluI AGCT 3 cut(s) 198, 288, 398
Alw21I GWGCWC 1 cut(s) 400
AlwI GGATC 1 cut(s) 464
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 2 cut(s) 149, 274
AspS9I GGNCC 2 cut(s) 256, 310
AsuHPI GGTGA 3 cut(s) 16, 79, 277
AsuII TTCGAA 1 cut(s) 484
AvaII GGWCC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 400
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 2 cut(s) 136, 261
BccI CCATC 2 cut(s) 256, 339
BceAI ACGGC 1 cut(s) 78
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BclI TGATCA 1 cut(s) 84
BfaI CTAG 1 cut(s) 125
BglII AGATCT 1 cut(s) 101
BisI GCNGC 5 cut(s) 48, 60, 150, 275, 297
BlsI GCNGC 5 cut(s) 49, 61, 151, 276, 298
BmcAI AGTACT 1 cut(s) 161
Bme18I GGWCC 1 cut(s) 256
BmgT120I GGNCC 2 cut(s) 256, 310
Bpu14I TTCGAA 1 cut(s) 484
BsaHI GRCGYC 1 cut(s) 281
BsaWI WCCGGW 1 cut(s) 392
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse118I RCCGGY 1 cut(s) 407
Bse3DI GCAATG 1 cut(s) 87
BseGI GGATG 1 cut(s) 350
BseLI CCNNNNNNNGG 1 cut(s) 359
BseMI GCAATG 1 cut(s) 87
BseXI GCAGC 2 cut(s) 136, 261
Bsh1236I CGCG 1 cut(s) 62
BshFI GGCC 1 cut(s) 311
BsiHKAI GWGCWC 1 cut(s) 400
BsiSI CCGG 2 cut(s) 393, 408
BslI CCNNNNNNNGG 1 cut(s) 359
BsnI GGCC 1 cut(s) 311
Bsp119I TTCGAA 1 cut(s) 484
Bsp1286I GDGCHC 1 cut(s) 400
Bsp143I GATC 5 cut(s) 84, 101, 168, 382, 469
BspACI CCGC 5 cut(s) 47, 60, 71, 296, 360
BspANI GGCC 1 cut(s) 311
BspFNI CGCG 1 cut(s) 62
BspHI TCATGA 1 cut(s) 491
BspPI GGATC 1 cut(s) 464
BspT104I TTCGAA 1 cut(s) 484
BsrBI CCGCTC 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 407
BssAI RCCGGY 1 cut(s) 407
BssMI GATC 5 cut(s) 84, 101, 168, 382, 469
BssNI GRCGYC 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 190
BstACI GRCGYC 1 cut(s) 281
BstAPI GCANNNNNTGC 1 cut(s) 56
BstBI TTCGAA 1 cut(s) 484
BstDEI CTNAG 1 cut(s) 132
BstF5I GGATG 1 cut(s) 350
BstFNI CGCG 1 cut(s) 62
BstKTI GATC 5 cut(s) 87, 104, 171, 385, 472
BstMBI GATC 5 cut(s) 84, 101, 168, 382, 469
BstMWI GCNNNNNNNGC 5 cut(s) 56, 68, 77, 302, 404
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 2 cut(s) 136, 261
BstX2I RGATCY 1 cut(s) 101
BstYI RGATCY 1 cut(s) 101
BsuRI GGCC 1 cut(s) 311
BtsCI GGATG 1 cut(s) 350
BtsI GCAGTG 2 cut(s) 306, 312
BtsIMutI CAGTG 2 cut(s) 306, 312
CciI TCATGA 1 cut(s) 491
Cfr10I RCCGGY 1 cut(s) 407
Cfr13I GGNCC 2 cut(s) 256, 310
Csp6I GTAC 2 cut(s) 35, 160
CviAII CATG 2 cut(s) 461, 492
CviJI RGCY 7 cut(s) 93, 131, 149, 198, 288, 311, 398
CviKI_1 RGCY 7 cut(s) 93, 131, 149, 198, 288, 311, 398
CviQI GTAC 2 cut(s) 35, 160
DdeI CTNAG 1 cut(s) 132
DpnI GATC 5 cut(s) 86, 103, 170, 384, 471
DpnII GATC 5 cut(s) 84, 101, 168, 382, 469
Ecl136II GAGCTC 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 256
Eco53kI GAGCTC 1 cut(s) 398
EcoICRI GAGCTC 1 cut(s) 398
EcoT38I GRGCYC 1 cut(s) 400
FaeI CATG 2 cut(s) 464, 495
FaiI YATR 6 cut(s) 138, 195, 235, 358, 462, 493
FatI CATG 2 cut(s) 460, 491
FbaI TGATCA 1 cut(s) 84
Fnu4HI GCNGC 5 cut(s) 48, 60, 150, 275, 297
FokI GGATG 1 cut(s) 357
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 5 cut(s) 48, 60, 150, 275, 297
FspBI CTAG 1 cut(s) 125
GluI GCNGC 5 cut(s) 48, 60, 150, 275, 297
HaeIII GGCC 1 cut(s) 311
HapII CCGG 2 cut(s) 393, 408
Hin1I GRCGYC 1 cut(s) 281
Hin1II CATG 2 cut(s) 464, 495
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HindIII AAGCTT 1 cut(s) 286
HpaI GTTAAC 1 cut(s) 12
HpaII CCGG 2 cut(s) 393, 408
HphI GGTGA 3 cut(s) 16, 79, 277
Hpy166II GTNNAC 1 cut(s) 12
Hpy188I TCNGA 2 cut(s) 372, 387
Hpy188III TCNNGA 1 cut(s) 492
Hpy8I GTNNAC 1 cut(s) 12
HpyAV CCTTC 1 cut(s) 118
HpyCH4III ACNGT 1 cut(s) 190
HpyCH4IV ACGT 2 cut(s) 281, 432
HpyCH4V TGCA 1 cut(s) 419
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 68, 77, 302, 404
HpyF3I CTNAG 1 cut(s) 132
HpySE526I ACGT 2 cut(s) 281, 432
Hsp92I GRCGYC 1 cut(s) 281
Hsp92II CATG 2 cut(s) 464, 495
Ksp22I TGATCA 1 cut(s) 84
KspAI GTTAAC 1 cut(s) 12
Kzo9I GATC 5 cut(s) 84, 101, 168, 382, 469
LmnI GCTCC 2 cut(s) 68, 395
LpnPI CCDG 5 cut(s) 103, 272, 327, 406, 421
Lsp1109I GCAGC 2 cut(s) 136, 261
MaeI CTAG 1 cut(s) 125
MaeII ACGT 2 cut(s) 281, 432
MaeIII GTNAC 2 cut(s) 184, 265
MalI GATC 5 cut(s) 86, 103, 170, 384, 471
MbiI CCGCTC 1 cut(s) 71
MboI GATC 5 cut(s) 84, 101, 168, 382, 469
MboII GAAGA 5 cut(s) 96, 206, 439, 470, 473
MflI RGATCY 1 cut(s) 101
MhlI GDGCHC 1 cut(s) 400
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 1 cut(s) 320
MseI TTAA 2 cut(s) 11, 156
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 2 cut(s) 393, 408
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 5 cut(s) 56, 68, 77, 302, 404
NdeII GATC 5 cut(s) 84, 101, 168, 382, 469
NlaIII CATG 2 cut(s) 464, 495
NmuCI GTSAC 2 cut(s) 184, 265
NspV TTCGAA 1 cut(s) 484
PagI TCATGA 1 cut(s) 491
PkrI GCNGC 5 cut(s) 49, 61, 151, 276, 298
Psp124BI GAGCTC 1 cut(s) 400
PspPI GGNCC 2 cut(s) 256, 310
PsuI RGATCY 1 cut(s) 101
RsaI GTAC 2 cut(s) 36, 161
RsaNI GTAC 2 cut(s) 35, 160
SacI GAGCTC 1 cut(s) 400
SaqAI TTAA 2 cut(s) 11, 156
SatI GCNGC 5 cut(s) 48, 60, 150, 275, 297
Sau3AI GATC 5 cut(s) 84, 101, 168, 382, 469
Sau96I GGNCC 2 cut(s) 256, 310
ScaI AGTACT 1 cut(s) 161
SduI GDGCHC 1 cut(s) 400
SetI ASST 6 cut(s) 200, 261, 284, 290, 400, 435
SfuI TTCGAA 1 cut(s) 484
SinI GGWCC 1 cut(s) 256
SsiI CCGC 5 cut(s) 47, 60, 71, 296, 360
SspMI CTAG 1 cut(s) 125
SstI GAGCTC 1 cut(s) 400
TaaI ACNGT 1 cut(s) 190
TaiI ACGT 2 cut(s) 284, 435
TaqI TCGA 1 cut(s) 484
TatI WGTACW 1 cut(s) 159
TauI GCSGC 3 cut(s) 50, 62, 299
Tru1I TTAA 2 cut(s) 11, 156
Tru9I TTAA 2 cut(s) 11, 156
TscAI CASTG 2 cut(s) 306, 312
TseFI GTSAC 2 cut(s) 184, 265
TseI GCWGC 2 cut(s) 149, 274
Tsp45I GTSAC 2 cut(s) 184, 265
TspRI CASTG 2 cut(s) 306, 312
VpaK11BI GGWCC 1 cut(s) 256
XspI CTAG 1 cut(s) 125
ZraI GACGTC 1 cut(s) 282
ZrmI AGTACT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.