Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008048

Physical Location & Seq

Genomic Coordinates Reverse (-)

44375511 .. 44376586

Gene Structure 1076 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1313000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 495 bp

ATGACTAAGAACAGGAGGATCTTCACCACTGTGCTGAGACTGCTAGCCTTGGCTGCGACTGTTTCTGCCACCATAGTTATGGTCACCAGCCACGACTCTACCAACGTCTTGAACTTGACCTTCAAGGCCAAGTACAACCATTCTCCGGCTTTCACGTACTTTGTGATAGTGGAGGCAATTATAAGTGGATACAACCTTATAATCCTGCTTCTTTCTTCCAAGGGCTCACTTTGGCGCGTGGTTATCATTCTAGATGTGGTTGCAGCTCTTCTTCTTACTTCAAGCATGTCAGCTGCCTTGGCGATAGCTCAAGTGGGCAAGAAAGGGAACACTCATGCTGGTTGGCTGCCAATTTGTGGACAGGTCTCCAAGTTTTGCAACCATGTCACTGGAGCTCTTGTTGCTGGCATCCTTGCAGCAATGTTTTACTTTGTGCTTAATCTGTTTACCCTTTACAATGTTCTTAATCCTCTATTTGTGGTAAAACCTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003824 GO:0005488 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009741 GO:0009826 GO:0009987 GO:0010033 GO:0014070 GO:0016043 GO:0016049 GO:0032501 GO:0032502 GO:0032989 GO:0033993 GO:0035264 GO:0040007 GO:0042221 GO:0048037 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0051536 GO:0051540 GO:0060560 GO:0071840 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF588

Protein Analysis

165

Amino Acids

17.77

Weight (kDa)

9.96

Isoelectric Point (pI)

24.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CASP_dom	PF04535	5 - 141	4.3e-38	Casparian strip membrane protein domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0008048)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03700 AT4G03540
fragaria_vesca	FvH4_3g09260
malus_domestica	MD05G1313000.v1.1 MD09G1247600.v1.1 MD10G1295200.v1.1 MD17G1240500.v1.1
prunus_persica	Prupe.3G137700_v2.0.a1 Prupe.3G137700_v2.0.a1 Prupe.4G046800_v2.0.a1
pyrus_communis	pycom09g16510 pycom10g24700 pycom17g24380
rosa_chinensis	RchiOBHm_Chr5g0008311
rosa_laevigata	RLG00000031561
rosa_multiflora	Rmu_sc0021840.1_g000002 Rmu_sc0031524.1_g000001
rosa_roxburghii	Rroxscaffold_1G00068150
rosa_rugosa	Rorug04G0435000
rosa_samantha	Rh5AG063700 Rh5BG060200 Rh5CG070600 Rh5DG059700
rosa_wichuraiana	Rw5G005720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	182, 200
AccB7I	CCANNNNNTGG	1 cut(s)	356
AccII	CGCG	1 cut(s)	237
AclWI	GGATC	1 cut(s)	26
AfaI	GTAC	2 cut(s)	134, 158
AfiI	CCNNNNNNNGG	2 cut(s)	145, 356
AgsI	TTSAA	3 cut(s)	112, 124, 282
AleI	CACNNNNGTG	1 cut(s)	29
AluBI	AGCT	4 cut(s)	266, 293, 308, 395
AluI	AGCT	4 cut(s)	266, 293, 308, 395
Alw21I	GWGCWC	1 cut(s)	397
Alw26I	GTCTC	2 cut(s)	31, 370
AlwI	GGATC	1 cut(s)	26
AoxI	GGCC	1 cut(s)	126
ApeKI	GCWGC	5 cut(s)	53, 263, 293, 346, 416
AspLEI	GCGC	1 cut(s)	237
AsuHPI	GGTGA	2 cut(s)	16, 76
AsuNHI	GCTAGC	1 cut(s)	43
BanII	GRGCYC	2 cut(s)	227, 397
Bbv12I	GWGCWC	1 cut(s)	397
BbvI	GCAGC	5 cut(s)	40, 275, 280, 333, 428
BciVI	GTATCC	1 cut(s)	182
BcoDI	GTCTC	2 cut(s)	31, 370
BfaI	CTAG	2 cut(s)	44, 251
BfuI	GTATCC	1 cut(s)	182
BisI	GCNGC	5 cut(s)	54, 264, 294, 347, 417
BlsI	GCNGC	5 cut(s)	55, 265, 295, 348, 418
BmsI	GCATC	1 cut(s)	417
BmtI	GCTAGC	1 cut(s)	47
BpmI	CTGGAG	1 cut(s)	411
BpuEI	CTTGAG	1 cut(s)	294
BsaAI	YACGTR	1 cut(s)	156
BsaI	GGTCTC	1 cut(s)	370
BsaJI	CCNNGG	3 cut(s)	48, 219, 297
BsaXI	ACNNNNNCTCC	2 cut(s)	384, 414
Bsc4I	CCNNNNNNNGG	2 cut(s)	145, 356
Bse1I	ACTGG	1 cut(s)	394
Bse3DI	GCAATG	1 cut(s)	426
BseDI	CCNNGG	3 cut(s)	48, 219, 297
BseGI	GGATG	1 cut(s)	408
BseLI	CCNNNNNNNGG	2 cut(s)	145, 356
BseMI	GCAATG	1 cut(s)	426
BseMII	CTCAG	1 cut(s)	26
BseNI	ACTGG	1 cut(s)	394
BseXI	GCAGC	5 cut(s)	40, 275, 280, 333, 428
Bsh1236I	CGCG	1 cut(s)	237
BshFI	GGCC	1 cut(s)	128
BsiHKAI	GWGCWC	1 cut(s)	397
BsiSI	CCGG	1 cut(s)	146
BslI	CCNNNNNNNGG	2 cut(s)	145, 356
BsmAI	GTCTC	2 cut(s)	31, 370
BsnI	GGCC	1 cut(s)	128
Bso31I	GGTCTC	1 cut(s)	370
Bsp1286I	GDGCHC	2 cut(s)	227, 397
Bsp143I	GATC	1 cut(s)	18
BspANI	GGCC	1 cut(s)	128
BspCNI	CTCAG	1 cut(s)	27
BspFNI	CGCG	1 cut(s)	237
BspOI	GCTAGC	1 cut(s)	47
BspPI	GGATC	1 cut(s)	26
BspQI	GCTCTTC	1 cut(s)	273
BspTNI	GGTCTC	1 cut(s)	370
BsrDI	GCAATG	1 cut(s)	426
BsrI	ACTGG	1 cut(s)	394
BssECI	CCNNGG	3 cut(s)	48, 219, 297
BssMI	GATC	1 cut(s)	18
BssT1I	CCWWGG	3 cut(s)	48, 219, 297
Bst4CI	ACNGT	2 cut(s)	31, 61
Bst6I	CTCTTC	1 cut(s)	273
BstBAI	YACGTR	1 cut(s)	156
BstC8I	GCNNGC	2 cut(s)	45, 406
BstDEI	CTNAG	2 cut(s)	6, 35
BstEII	GGTNACC	1 cut(s)	82
BstF5I	GGATG	1 cut(s)	408
BstFNI	CGCG	1 cut(s)	237
BstHHI	GCGC	1 cut(s)	237
BstKTI	GATC	1 cut(s)	21
BstMAI	GTCTC	2 cut(s)	31, 370
BstMBI	GATC	1 cut(s)	18
BstMWI	GCNNNNNNNGC	4 cut(s)	40, 53, 299, 401
BstNSI	RCATGY	1 cut(s)	289
BstPI	GGTNACC	1 cut(s)	82
BstUI	CGCG	1 cut(s)	237
BstV1I	GCAGC	5 cut(s)	40, 275, 280, 333, 428
BstX2I	RGATCY	1 cut(s)	18
BstXI	CCANNNNNNTGG	2 cut(s)	79, 389
BstYI	RGATCY	1 cut(s)	18
BsuI	GTATCC	1 cut(s)	182
BsuRI	GGCC	1 cut(s)	128
BtsCI	GGATG	1 cut(s)	408
BtsIMutI	CAGTG	2 cut(s)	27, 387
Cac8I	GCNNGC	2 cut(s)	45, 406
CfoI	GCGC	1 cut(s)	237
Csp6I	GTAC	2 cut(s)	133, 157
CviAII	CATG	3 cut(s)	286, 335, 383
CviQI	GTAC	2 cut(s)	133, 157
DdeI	CTNAG	2 cut(s)	6, 35
DpnI	GATC	1 cut(s)	20
DpnII	GATC	1 cut(s)	18
Eam1104I	CTCTTC	1 cut(s)	273
EarI	CTCTTC	1 cut(s)	273
Ecl136II	GAGCTC	1 cut(s)	395
Eco130I	CCWWGG	3 cut(s)	48, 219, 297
Eco24I	GRGCYC	2 cut(s)	227, 397
Eco31I	GGTCTC	1 cut(s)	370
Eco53kI	GAGCTC	1 cut(s)	395
Eco91I	GGTNACC	1 cut(s)	82
EcoICRI	GAGCTC	1 cut(s)	395
EcoO65I	GGTNACC	1 cut(s)	82
EcoT14I	CCWWGG	3 cut(s)	48, 219, 297
EcoT38I	GRGCYC	2 cut(s)	227, 397
ErhI	CCWWGG	3 cut(s)	48, 219, 297
FaeI	CATG	3 cut(s)	289, 338, 386
FaiI	YATR	7 cut(s)	74, 80, 182, 200, 287, 336, 384
FatI	CATG	3 cut(s)	285, 334, 382
Fnu4HI	GCNGC	5 cut(s)	54, 264, 294, 347, 417
FokI	GGATG	1 cut(s)	395
FriOI	GRGCYC	2 cut(s)	227, 397
Fsp4HI	GCNGC	5 cut(s)	54, 264, 294, 347, 417
FspBI	CTAG	2 cut(s)	44, 251
GlaI	GCGC	1 cut(s)	236
GluI	GCNGC	5 cut(s)	54, 264, 294, 347, 417
GsuI	CTGGAG	1 cut(s)	411
HaeIII	GGCC	1 cut(s)	128
HapII	CCGG	1 cut(s)	146
HhaI	GCGC	1 cut(s)	237
Hin1II	CATG	3 cut(s)	289, 338, 386
Hin6I	GCGC	1 cut(s)	235
HinP1I	GCGC	1 cut(s)	235
HinfI	GANTC	1 cut(s)	95
HpaII	CCGG	1 cut(s)	146
HphI	GGTGA	2 cut(s)	16, 76
Hpy166II	GTNNAC	2 cut(s)	359, 447
Hpy188III	TCNNGA	2 cut(s)	109, 251
Hpy8I	GTNNAC	2 cut(s)	359, 447
HpyAV	CCTTC	1 cut(s)	130
HpyCH4III	ACNGT	2 cut(s)	31, 61
HpyCH4IV	ACGT	2 cut(s)	105, 155
HpyCH4V	TGCA	3 cut(s)	263, 378, 416
HpyF10VI	GCNNNNNNNGC	4 cut(s)	40, 53, 299, 401
HpyF3I	CTNAG	2 cut(s)	6, 35
HpySE526I	ACGT	2 cut(s)	105, 155
Hsp92II	CATG	3 cut(s)	289, 338, 386
HspAI	GCGC	1 cut(s)	235
Kzo9I	GATC	1 cut(s)	18
LguI	GCTCTTC	1 cut(s)	273
LmnI	GCTCC	1 cut(s)	392
LpnPI	CCDG	7 cut(s)	100, 159, 218, 324, 347, 375, 390
Lsp1109I	GCAGC	5 cut(s)	40, 275, 280, 333, 428
LweI	GCATC	1 cut(s)	417
MaeI	CTAG	2 cut(s)	44, 251
MaeII	ACGT	2 cut(s)	105, 155
MaeIII	GTNAC	2 cut(s)	82, 385
MalI	GATC	1 cut(s)	20
MboI	GATC	1 cut(s)	18
MboII	GAAGA	4 cut(s)	13, 207, 260, 263
MflI	RGATCY	1 cut(s)	18
MhlI	GDGCHC	2 cut(s)	227, 397
MluCI	AATT	3 cut(s)	177, 351, 490
MlyI	GAGTC	1 cut(s)	89
MnlI	CCTC	3 cut(s)	9, 166, 480
MseI	TTAA	3 cut(s)	438, 465, 493
MslI	CAYNNNNRTG	2 cut(s)	29, 77
MspA1I	CMGCKG	1 cut(s)	293
MspI	CCGG	1 cut(s)	146
MvnI	CGCG	1 cut(s)	237
MwoI	GCNNNNNNNGC	4 cut(s)	40, 53, 299, 401
NdeII	GATC	1 cut(s)	18
NheI	GCTAGC	1 cut(s)	43
NlaIII	CATG	3 cut(s)	289, 338, 386
NmuCI	GTSAC	2 cut(s)	82, 385
NspI	RCATGY	1 cut(s)	289
OliI	CACNNNNGTG	1 cut(s)	29
PciSI	GCTCTTC	1 cut(s)	273
PflMI	CCANNNNNTGG	1 cut(s)	356
PkrI	GCNGC	5 cut(s)	55, 265, 295, 348, 418
PleI	GAGTC	1 cut(s)	89
PpsI	GAGTC	1 cut(s)	89
Ppu21I	YACGTR	1 cut(s)	156
PsiI	TTATAA	2 cut(s)	182, 200
Psp124BI	GAGCTC	1 cut(s)	397
PspEI	GGTNACC	1 cut(s)	82
PsuI	RGATCY	1 cut(s)	18
PvuII	CAGCTG	1 cut(s)	293
RsaI	GTAC	2 cut(s)	134, 158
RsaNI	GTAC	2 cut(s)	133, 157
RseI	CAYNNNNRTG	2 cut(s)	29, 77
SacI	GAGCTC	1 cut(s)	397
SapI	GCTCTTC	1 cut(s)	273
SaqAI	TTAA	3 cut(s)	438, 465, 493
SatI	GCNGC	5 cut(s)	54, 264, 294, 347, 417
Sau3AI	GATC	1 cut(s)	18
SchI	GAGTC	1 cut(s)	89
SduI	GDGCHC	2 cut(s)	227, 397
SfaNI	GCATC	1 cut(s)	417
SmiMI	CAYNNNNRTG	2 cut(s)	29, 77
SmlI	CTYRAG	1 cut(s)	309
SmoI	CTYRAG	1 cut(s)	309
Sse9I	AATT	3 cut(s)	177, 351, 490
SspMI	CTAG	2 cut(s)	44, 251
SstI	GAGCTC	1 cut(s)	397
StyI	CCWWGG	3 cut(s)	48, 219, 297
TaaI	ACNGT	2 cut(s)	31, 61
TaiI	ACGT	2 cut(s)	108, 158
TasI	AATT	3 cut(s)	177, 351, 490
TatI	WGTACW	1 cut(s)	132
Tru1I	TTAA	3 cut(s)	438, 465, 493
Tru9I	TTAA	3 cut(s)	438, 465, 493
TscAI	CASTG	2 cut(s)	34, 394
TseFI	GTSAC	2 cut(s)	82, 385
TseI	GCWGC	5 cut(s)	53, 263, 293, 346, 416
Tsp45I	GTSAC	2 cut(s)	82, 385
TspRI	CASTG	2 cut(s)	34, 394
Van91I	CCANNNNNTGG	1 cut(s)	356
XbaI	TCTAGA	1 cut(s)	250
XceI	RCATGY	1 cut(s)	289
XcmI	CCANNNNNNNNNTGG	1 cut(s)	76
XspI	CTAG	2 cut(s)	44, 251

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.