MD09G1247600.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008048
Physical Location & Seq
Reverse (-)
31769174 .. 31770318
1145 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1247600.v1.1.491

Sequence Viewer

Length: 522 bp
ATGCACCCAAACTCCAGTGCAGAATCCATGGCCAAGATCACTAAGAGAGTCTGCACCCTAGTACTACGGCTGATGGCCGCCGCCGCCACCCTTTTGGCTGCAATTGTCATGGTTACAAGCCATCAGAGAACCAGCATCTTTGGAGTATCTTTTGAAGTCAAGTATAACCACACACCAGCCTTCCAGTACTTTGTGATAGCAAATGCGATTGCAACTGTATACGGGTTTTTGGTTCTTTTTCTTCCTTCTGAGAGTCAGCTATGGAGATTAGTTGTGGCTTTGGATGTGGTAATCACTGTATTACTGAGCTCAAGCATTTCAGCAGCTTTGGCTATTGGTGATGTAGGGAAGCATGGAAATTCATATGCAGGTTGGCAACCGATATGTGGGCAAGTCCCCAAATACTGTGACCAAGTAAAGGCAGCCCTAATCTCAAGCTTTGTTGGCCTCATAATATATTTGCTGCTGCTTCTGTATGCCATCCACACTGTCCTGGATCCTCTTCTCTTGAAAAAAACTTAA

Protein Analysis

174

Amino Acids

18.77

Weight (kDa)

9.22

Isoelectric Point (pI)

32.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 16 - 151 1.1e-39 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 359
AccI GTMKAC 1 cut(s) 219
AciI CCGC 3 cut(s) 78, 81, 84
AclWI GGATC 2 cut(s) 491, 504
AcoI YGGCCR 2 cut(s) 30, 75
AcsI RAATTY 1 cut(s) 358
AfaI GTAC 2 cut(s) 63, 188
AfiI CCNNNNNNNGG 2 cut(s) 386, 418
AgsI TTSAA 2 cut(s) 155, 511
AjnI CCWGG 1 cut(s) 492
AluBI AGCT 4 cut(s) 259, 309, 326, 438
AluI AGCT 4 cut(s) 259, 309, 326, 438
Alw21I GWGCWC 1 cut(s) 311
AlwI GGATC 2 cut(s) 491, 504
AoxI GGCC 3 cut(s) 30, 75, 445
ApeKI GCWGC 5 cut(s) 98, 323, 422, 463, 466
ApoI RAATTY 1 cut(s) 358
AsuHPI GGTGA 1 cut(s) 350
BalI TGGCCA 1 cut(s) 32
BamHI GGATCC 1 cut(s) 496
BanII GRGCYC 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 311
BbvI GCAGC 5 cut(s) 85, 335, 434, 450, 453
BccI CCATC 3 cut(s) 67, 129, 488
BceAI ACGGC 1 cut(s) 83
BciT130I CCWGG 1 cut(s) 494
BfaI CTAG 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 359
BisI GCNGC 8 cut(s) 78, 81, 84, 99, 324, 423, 464, 467
BlsI GCNGC 8 cut(s) 79, 82, 85, 100, 325, 424, 465, 468
BmcAI AGTACT 2 cut(s) 63, 188
Bme1390I CCNGG 1 cut(s) 494
BmiI GGNNCC 1 cut(s) 498
BmrFI CCNGG 1 cut(s) 494
BmsI GCATC 1 cut(s) 144
BpuEI CTTGAG 2 cut(s) 295, 418
BsaJI CCNNGG 1 cut(s) 27
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 386, 418
Bse1I ACTGG 2 cut(s) 15, 184
BseBI CCWGG 1 cut(s) 494
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 2 cut(s) 289, 480
BseLI CCNNNNNNNGG 2 cut(s) 386, 418
BseMII CTCAG 2 cut(s) 240, 296
BseNI ACTGG 2 cut(s) 15, 184
BseXI GCAGC 5 cut(s) 85, 335, 434, 450, 453
BsgI GTGCAG 2 cut(s) 37, 39
BshFI GGCC 3 cut(s) 32, 77, 447
BsiHKAI GWGCWC 1 cut(s) 311
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 2 cut(s) 386, 418
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 3 cut(s) 32, 77, 447
Bsp1286I GDGCHC 1 cut(s) 311
Bsp143I GATC 2 cut(s) 36, 496
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 3 cut(s) 78, 81, 84
BspANI GGCC 3 cut(s) 32, 77, 447
BspCNI CTCAG 2 cut(s) 241, 297
BspLI GGNNCC 1 cut(s) 498
BspMI ACCTGC 1 cut(s) 359
BspPI GGATC 2 cut(s) 491, 504
BsrI ACTGG 2 cut(s) 15, 184
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 2 cut(s) 36, 496
BssNAI GTATAC 1 cut(s) 220
BssT1I CCWWGG 1 cut(s) 27
Bst1107I GTATAC 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 494
Bst4CI ACNGT 4 cut(s) 217, 298, 407, 490
Bst6I CTCTTC 1 cut(s) 507
BstDEI CTNAG 3 cut(s) 42, 249, 305
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 2 cut(s) 289, 480
BstKTI GATC 2 cut(s) 39, 499
BstMBI GATC 2 cut(s) 36, 496
BstMWI GCNNNNNNNGC 3 cut(s) 83, 329, 444
BstNI CCWGG 1 cut(s) 494
BstSCI CCNGG 1 cut(s) 492
BstV1I GCAGC 5 cut(s) 85, 335, 434, 450, 453
BstX2I RGATCY 1 cut(s) 496
BstXI CCANNNNNNTGG 1 cut(s) 94
BstYI RGATCY 1 cut(s) 496
BstZ17I GTATAC 1 cut(s) 220
BsuRI GGCC 3 cut(s) 32, 77, 447
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 2 cut(s) 289, 480
BtsIMutI CAGTG 3 cut(s) 22, 294, 486
BveI ACCTGC 1 cut(s) 359
Csp6I GTAC 2 cut(s) 62, 187
CviAII CATG 3 cut(s) 28, 109, 353
CviQI GTAC 2 cut(s) 62, 187
DdeI CTNAG 3 cut(s) 42, 249, 305
DpnI GATC 2 cut(s) 38, 498
DpnII GATC 2 cut(s) 36, 496
EaeI YGGCCR 2 cut(s) 30, 75
Eam1104I CTCTTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 507
Ecl136II GAGCTC 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 27
Eco24I GRGCYC 1 cut(s) 311
Eco53kI GAGCTC 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 492
EcoT14I CCWWGG 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 311
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 3 cut(s) 31, 112, 356
FaqI GGGAC 1 cut(s) 380
FatI CATG 3 cut(s) 27, 108, 352
FauNDI CATATG 1 cut(s) 364
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 8 cut(s) 78, 81, 84, 99, 324, 423, 464, 467
FokI GGATG 2 cut(s) 296, 467
FriOI GRGCYC 1 cut(s) 311
Fsp4HI GCNGC 8 cut(s) 78, 81, 84, 99, 324, 423, 464, 467
FspBI CTAG 1 cut(s) 59
GluI GCNGC 8 cut(s) 78, 81, 84, 99, 324, 423, 464, 467
HaeIII GGCC 3 cut(s) 32, 77, 447
Hin1II CATG 3 cut(s) 31, 112, 356
HindIII AAGCTT 1 cut(s) 436
HinfI GANTC 3 cut(s) 23, 48, 253
HphI GGTGA 1 cut(s) 350
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 2 cut(s) 126, 250
Hpy188III TCNNGA 1 cut(s) 508
Hpy8I GTNNAC 1 cut(s) 220
HpyAV CCTTC 2 cut(s) 190, 255
HpyCH4III ACNGT 4 cut(s) 217, 298, 407, 490
HpyCH4V TGCA 6 cut(s) 4, 20, 54, 101, 212, 368
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 329, 444
HpyF3I CTNAG 3 cut(s) 42, 249, 305
Hsp92II CATG 3 cut(s) 31, 112, 356
Kzo9I GATC 2 cut(s) 36, 496
LpnPI CCDG 7 cut(s) 28, 145, 189, 197, 354, 479, 506
Lsp1109I GCAGC 5 cut(s) 85, 335, 434, 450, 453
LweI GCATC 1 cut(s) 144
MaeI CTAG 1 cut(s) 59
MaeIII GTNAC 2 cut(s) 112, 407
MalI GATC 2 cut(s) 38, 498
MboI GATC 2 cut(s) 36, 496
MboII GAAGA 2 cut(s) 233, 494
MfeI CAATTG 1 cut(s) 102
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 1 cut(s) 311
MlsI TGGCCA 1 cut(s) 32
MluCI AATT 2 cut(s) 102, 358
MluNI TGGCCA 1 cut(s) 32
MlyI GAGTC 2 cut(s) 57, 262
MnlI CCTC 2 cut(s) 458, 510
Mox20I TGGCCA 1 cut(s) 32
MscI TGGCCA 1 cut(s) 32
MseI TTAA 1 cut(s) 520
Msp20I TGGCCA 1 cut(s) 32
MspR9I CCNGG 1 cut(s) 494
MunI CAATTG 1 cut(s) 102
MvaI CCWGG 1 cut(s) 494
MwoI GCNNNNNNNGC 3 cut(s) 83, 329, 444
NcoI CCATGG 1 cut(s) 27
NdeI CATATG 1 cut(s) 364
NdeII GATC 2 cut(s) 36, 496
NlaIII CATG 3 cut(s) 31, 112, 356
NlaIV GGNNCC 1 cut(s) 498
NmuCI GTSAC 1 cut(s) 407
PfeI GAWTC 1 cut(s) 23
PfoI TCCNGGA 1 cut(s) 492
PkrI GCNGC 8 cut(s) 79, 82, 85, 100, 325, 424, 465, 468
PleI GAGTC 2 cut(s) 56, 261
PpsI GAGTC 2 cut(s) 56, 261
Psp124BI GAGCTC 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 492
PspGI CCWGG 1 cut(s) 492
PspN4I GGNNCC 1 cut(s) 498
PsuI RGATCY 1 cut(s) 496
RsaI GTAC 2 cut(s) 63, 188
RsaNI GTAC 2 cut(s) 62, 187
SacI GAGCTC 1 cut(s) 311
SaqAI TTAA 1 cut(s) 520
SatI GCNGC 8 cut(s) 78, 81, 84, 99, 324, 423, 464, 467
Sau3AI GATC 2 cut(s) 36, 496
ScaI AGTACT 2 cut(s) 63, 188
SchI GAGTC 2 cut(s) 57, 262
ScrFI CCNGG 1 cut(s) 494
SduI GDGCHC 1 cut(s) 311
SetI ASST 5 cut(s) 261, 311, 328, 373, 440
SfaNI GCATC 1 cut(s) 144
SmlI CTYRAG 2 cut(s) 310, 433
SmoI CTYRAG 2 cut(s) 310, 433
Sse9I AATT 2 cut(s) 102, 358
SsiI CCGC 3 cut(s) 78, 81, 84
SspMI CTAG 1 cut(s) 59
SstI GAGCTC 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 492
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 4 cut(s) 217, 298, 407, 490
TasI AATT 2 cut(s) 102, 358
TatI WGTACW 2 cut(s) 61, 186
TauI GCSGC 3 cut(s) 80, 83, 86
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 1 cut(s) 520
Tru9I TTAA 1 cut(s) 520
TscAI CASTG 3 cut(s) 22, 301, 493
TseFI GTSAC 1 cut(s) 407
TseI GCWGC 5 cut(s) 98, 323, 422, 463, 466
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 1 cut(s) 351
TspRI CASTG 3 cut(s) 22, 301, 493
XapI RAATTY 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 219
XspI CTAG 1 cut(s) 59
ZrmI AGTACT 2 cut(s) 63, 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.