RchiOBHm_Chr5g0008311

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008048
Physical Location & Seq
Forward (+)
5304572 .. 5305389
818 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28929

Sequence Viewer

Length: 495 bp
ATGAACACCAGCAGGAGGATCTTCACCTTTCTGCTCAGGCTACTAGCCTTGGCTGCAACTCTGGTTGCCACCATTGTAATGGTTACAAGCCATGACTCTACGAATGTCTTGAACTTAACGTTTAAGGCCAAATTCAGCAACTCACCAGCTTTCAAGTACTTTGTGGTAGTGGAAGCAATTGCCTGCGCGTACAACCTTGTCATTATGTTTCTTGCTTCCAAGGGCTCGCTTTGGCGCTTGGTTATCATCCTCGACGTGGTTGTAGCGGTGTTACTTGCTTCAAGTGTGTCATCGGCCTTGGCAATAGCATATGTGGGGAAGAAAGGAAACACACACGCTGGTTGGCTTCCGATTTGTGGACAAGTCCCAAAGTTCTGCAAGCATGTCACTGCTGCTCTTGTTGCTGGCTTCATTGCAGCAATCCTGTATTTTCTTCTTGTTATCTATACCCTCTACACTGTTCTCCATCCTCTATTTGTTGTAAGTAAGTTTTGA

Protein Analysis

164

Amino Acids

17.86

Weight (kDa)

9.86

Isoelectric Point (pI)

25.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 4 - 142 7.9e-41 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 188
AciI CCGC 1 cut(s) 266
AclI AACGTT 1 cut(s) 119
AclWI GGATC 1 cut(s) 26
AcsI RAATTY 1 cut(s) 131
AfaI GTAC 2 cut(s) 158, 191
AfiI CCNNNNNNNGG 3 cut(s) 15, 256, 356
AgsI TTSAA 3 cut(s) 112, 154, 282
AjiI CACGTC 1 cut(s) 256
AluBI AGCT 1 cut(s) 149
AluI AGCT 1 cut(s) 149
AlwI GGATC 1 cut(s) 26
AoxI GGCC 2 cut(s) 126, 294
ApeKI GCWGC 3 cut(s) 53, 392, 416
ApoI RAATTY 1 cut(s) 131
AspLEI GCGC 2 cut(s) 188, 237
AsuHPI GGTGA 2 cut(s) 16, 135
BanII GRGCYC 1 cut(s) 227
BbvI GCAGC 3 cut(s) 40, 379, 428
BccI CCATC 1 cut(s) 474
BfaI CTAG 1 cut(s) 44
BfoI RGCGCY 1 cut(s) 238
BisI GCNGC 3 cut(s) 54, 393, 417
BlsI GCNGC 3 cut(s) 55, 394, 418
BmcAI AGTACT 1 cut(s) 158
BmgBI CACGTC 1 cut(s) 256
Bpu10I CCTNAGC 1 cut(s) 35
BsaJI CCNNGG 3 cut(s) 48, 219, 297
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 256, 356
Bse3DI GCAATG 1 cut(s) 411
BseDI CCNNGG 3 cut(s) 48, 219, 297
BseGI GGATG 2 cut(s) 246, 466
BseLI CCNNNNNNNGG 3 cut(s) 15, 256, 356
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 1 cut(s) 49
BseXI GCAGC 3 cut(s) 40, 379, 428
Bsh1236I CGCG 1 cut(s) 188
BshFI GGCC 2 cut(s) 128, 296
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 3 cut(s) 15, 256, 356
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 2 cut(s) 128, 296
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 1 cut(s) 18
BspACI CCGC 1 cut(s) 266
BspANI GGCC 2 cut(s) 128, 296
BspCNI CTCAG 1 cut(s) 48
BspFNI CGCG 1 cut(s) 188
BspPI GGATC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 411
BssECI CCNNGG 3 cut(s) 48, 219, 297
BssMI GATC 1 cut(s) 18
BssT1I CCWWGG 3 cut(s) 48, 219, 297
Bst4CI ACNGT 1 cut(s) 460
BstC8I GCNNGC 4 cut(s) 184, 227, 380, 406
BstDEI CTNAG 1 cut(s) 35
BstF5I GGATG 2 cut(s) 246, 466
BstFNI CGCG 1 cut(s) 188
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 2 cut(s) 188, 237
BstKTI GATC 1 cut(s) 21
BstMBI GATC 1 cut(s) 18
BstMWI GCNNNNNNNGC 2 cut(s) 53, 401
BstNSI RCATGY 1 cut(s) 386
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 3 cut(s) 40, 379, 428
BstX2I RGATCY 1 cut(s) 18
BstXI CCANNNNNNTGG 1 cut(s) 79
BstYI RGATCY 1 cut(s) 18
BsuRI GGCC 2 cut(s) 128, 296
BtrI CACGTC 1 cut(s) 256
BtsCI GGATG 2 cut(s) 246, 466
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 2 cut(s) 387, 456
Cac8I GCNNGC 4 cut(s) 184, 227, 380, 406
CfoI GCGC 2 cut(s) 188, 237
Csp6I GTAC 2 cut(s) 157, 190
CviAII CATG 2 cut(s) 92, 383
CviQI GTAC 2 cut(s) 157, 190
DdeI CTNAG 1 cut(s) 35
DpnI GATC 1 cut(s) 20
DpnII GATC 1 cut(s) 18
Eco130I CCWWGG 3 cut(s) 48, 219, 297
Eco24I GRGCYC 1 cut(s) 227
EcoT14I CCWWGG 3 cut(s) 48, 219, 297
EcoT38I GRGCYC 1 cut(s) 227
ErhI CCWWGG 3 cut(s) 48, 219, 297
FaeI CATG 2 cut(s) 95, 386
FaiI YATR 6 cut(s) 93, 206, 310, 312, 384, 447
FaqI GGGAC 1 cut(s) 350
FatI CATG 2 cut(s) 91, 382
FauNDI CATATG 1 cut(s) 310
Fnu4HI GCNGC 3 cut(s) 54, 393, 417
FokI GGATG 2 cut(s) 233, 453
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 3 cut(s) 54, 393, 417
FspBI CTAG 1 cut(s) 44
GlaI GCGC 2 cut(s) 187, 236
GluI GCNGC 3 cut(s) 54, 393, 417
HaeII RGCGCY 1 cut(s) 238
HaeIII GGCC 2 cut(s) 128, 296
HhaI GCGC 2 cut(s) 188, 237
Hin1II CATG 2 cut(s) 95, 386
Hin6I GCGC 2 cut(s) 186, 235
HinP1I GCGC 2 cut(s) 186, 235
HinfI GANTC 1 cut(s) 95
HphI GGTGA 2 cut(s) 16, 135
Hpy166II GTNNAC 1 cut(s) 359
Hpy188I TCNGA 1 cut(s) 351
Hpy188III TCNNGA 1 cut(s) 109
Hpy8I GTNNAC 1 cut(s) 359
Hpy99I CGWCG 1 cut(s) 257
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4IV ACGT 2 cut(s) 119, 255
HpyCH4V TGCA 3 cut(s) 56, 378, 416
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 401
HpyF3I CTNAG 1 cut(s) 35
HpySE526I ACGT 2 cut(s) 119, 255
Hsp92II CATG 2 cut(s) 95, 386
HspAI GCGC 2 cut(s) 186, 235
Kzo9I GATC 1 cut(s) 18
LpnPI CCDG 8 cut(s) 22, 22, 47, 159, 196, 324, 390, 437
Lsp1109I GCAGC 3 cut(s) 40, 379, 428
MaeI CTAG 1 cut(s) 44
MaeII ACGT 2 cut(s) 119, 255
MaeIII GTNAC 3 cut(s) 82, 270, 385
MalI GATC 1 cut(s) 20
MboI GATC 1 cut(s) 18
MboII GAAGA 3 cut(s) 13, 331, 425
MfeI CAATTG 1 cut(s) 177
MflI RGATCY 1 cut(s) 18
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 2 cut(s) 131, 177
MlyI GAGTC 1 cut(s) 89
MnlI CCTC 4 cut(s) 9, 260, 461, 480
MseI TTAA 2 cut(s) 116, 123
MslI CAYNNNNRTG 1 cut(s) 77
MunI CAATTG 1 cut(s) 177
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 2 cut(s) 53, 401
NdeI CATATG 1 cut(s) 310
NdeII GATC 1 cut(s) 18
NlaIII CATG 2 cut(s) 95, 386
NmuCI GTSAC 1 cut(s) 385
NspI RCATGY 1 cut(s) 386
PkrI GCNGC 3 cut(s) 55, 394, 418
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp1406I AACGTT 1 cut(s) 119
PsuI RGATCY 1 cut(s) 18
RsaI GTAC 2 cut(s) 158, 191
RsaNI GTAC 2 cut(s) 157, 190
RseI CAYNNNNRTG 1 cut(s) 77
SaqAI TTAA 2 cut(s) 116, 123
SatI GCNGC 3 cut(s) 54, 393, 417
Sau3AI GATC 1 cut(s) 18
ScaI AGTACT 1 cut(s) 158
SchI GAGTC 1 cut(s) 89
SduI GDGCHC 1 cut(s) 227
SetI ASST 5 cut(s) 29, 122, 151, 198, 258
SmiMI CAYNNNNRTG 1 cut(s) 77
Sse9I AATT 2 cut(s) 131, 177
SsiI CCGC 1 cut(s) 266
SspMI CTAG 1 cut(s) 44
StyI CCWWGG 3 cut(s) 48, 219, 297
TaaI ACNGT 1 cut(s) 460
TaiI ACGT 2 cut(s) 122, 258
TaqI TCGA 1 cut(s) 252
TasI AATT 2 cut(s) 131, 177
TatI WGTACW 1 cut(s) 156
Tru1I TTAA 2 cut(s) 116, 123
Tru9I TTAA 2 cut(s) 116, 123
TscAI CASTG 2 cut(s) 394, 463
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 3 cut(s) 53, 392, 416
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 2 cut(s) 17, 400
TspRI CASTG 2 cut(s) 394, 463
XapI RAATTY 1 cut(s) 131
XceI RCATGY 1 cut(s) 386
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XspI CTAG 1 cut(s) 44
ZrmI AGTACT 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.